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At least 1,153 records · Page 64
All sourcesNASA NTRSDOE OSTI
DOE OSTI2020

Metamaterials for Mechanically Directing Cell Phenotype

Recently, abundant research has begun to reveal how microscale surface topography can influence a wide range of cell phenotypes in vitro, such as cell mobility, cell size and cell viability (Flemming et al. 1999, Nikkhah et al. 2012, Wang et al. 2016). However, while these studies shed some light on cell interactions with 2D surfaces of different length scales, cells in vivo experience their surroundings in 3D. Therefore, investigations into cell interactions with varying 3D microstructures are needed to gain biologically relevant insight into how microarchitecture and feature size influences cell behavior. Understanding this relationship between 3D microstructure and cell behavior will have a huge impact on tissue engineering and mechanobiology. Until now, the ability to fabricate complex 3D microstructures for cell behavior studies has been limited. This study harnessed LLNL’s Projection Micro-Stereolithography (PμSL) capability to probe the effects of micron-scale changes in 3D architecture on cell phenotype. Furthermore, our studies explored the application of external forces—such as cyclic loading and flow-induced shear stress—to cell-seeded 3D microstructures to further examine cell behavior.

36 MATERIALS SCIENCE↗
NASA NTRS1984

On the stability of the ionopause of Venus

The stability of the Venus ionopause is examined in light of the importance of gravitation and curvature. Using a one-fluid approximation for the equation of motion of the plasma, and ignoring the effects of neutrals, a dispersion relation is obtained that includes the effects of the magnetic field, sheared plasma flow, buoyancy, centrifugal force and magnetic tension due to boundary curvature. It is found that buoyancy acts to neutralize the flute instability. As expected, the Kelvin-Helmholtz mode is the dominant instability over most of the dayside ionopause. The expected growth times of this mode are short in comparison with the wave-convection time over the boundary; the waves can grow and saturate quickly, producing a turbulent boundary that may affect electrodynamic coupling between the solar wind and ionospheric plasmas.

Elphic, R. C.↗
NASA NTRS1985

Features of a reattaching turbulent shear layer in divergent channel flow

Experimental data have been obtained in an incompressible turbulent flow over a rearward-facing step in a diverging channel flow. Mean velocities, Reynolds stresses, and triple products that were measured by a laser Doppler velocimeter are presented for two cases of tunnel wall divergence. Eddy viscosities, production, convection, turbulent diffusion, and dissipation (balance of kinetic energy equation) terms are extracted from the data. These data are compared with various eddy-viscosity turbulence models. Numerical calculations incorporating the k-epsilon and algebraic-stress turbulence models are compared with the data. When determining quantities of engineering interest, the modified algebraic-stress model (ASM) is a significant improvement over the unmodified ASM and the unmodified k-epsilon model; however, like the others, it dramatically overpredicts the experimentally determined dissipation rate.

Driver, D. M.↗
NASA NTRS1985

Numerical procedure for three-dimensional hypersonic viscous flow over aerobrake configuration

A numerical method, which is simpler and more efficient than others currently in use, is proposed for the computation of the full viscous flow over an aerobrake body in hypersonic stream at high altitude. It treats the shock layer surrounding the blunt forebody and the near wake behind the base simultaneously by formulating the Navier-Stokes equations in conformal and azimuthal-angle coordinates. The computational domain is confined by the body wall, outflow surface and the shock, which is adjusted along the coordinate normal to the wall in the course of iterations. Because of the optimal grid and a well developed alternating direction implicit factorization technique for the governing equations, reasonably accurate results can be obtained with a 28 x 36 x 7 grid and 400 time-marching iterations. Excellent agreement of shock location is found between the present result and the schlieren photograph. Details of the base flow and shear layer impingement on the cylindrical aft body are presented for an adiabatic wall case.

Li, C. P.↗
NASA NTRS1987

Conditions for double layers in the Earth's magnetosphere and perhaps in other astrophysical objects

Double layers form along auroral field lines in the Earth's magnetosphere. They form in order to maintain current continuity in the ionosphere in the presence of a magnetospheric electric field E with nabla x E is not equal to 0. Features which govern the formation of the double layers are: (1) the divergence of E, (2) the conductivity of the ionosphere, and (3) the current-voltage characteristics of auroral magnetic field lines. Astrophysical situations where nabla x E is not equal to 0 is applied to a conducting plasma similar to the Earth's ionosphere are potential candidates for the formation of double layers. The region with nabla x E is not equal to 0 can be generated within, or along field lines connected to, the conducting plasma. In addition to nabla x E, shear neutral flow in the conducting plasma can also form double layers.

Lyons, L. R.↗
NASA NTRS1986

Turbulent solutions of the Navier-Stokes equations

Analytical and numerical approaches to the mechanics of turbulent flow are examined in a general introduction and illustrated with graphs. Topics discussed include the averaged and unaveraged basic equations, numerical solutions and methods, homogeneous fluctuations and turbulence with no mean flow, uniformly sheared fluctuations and turbulence, inhomogeneous fluctuations and turbulence in a developing shear layer, and steady-state homogeneous turbulence with a spatially periodic body force.

Deissler, R. G.↗
NASA NTRS1987

Analysis and simulations of a troposphere-stratosphere gravity wave model. I

An analytical model is presented that accommodates nonhydrostatic shearing stratified flow over an obstacle, and that can be modified to include a superposed stratosphere with constant wind and higher stability. A simulation code is used in parallel with the analytic calculations to demonstrate a methodology for determining the wavelength and magnitude of gravity wave energy reflected, and that transmitted, by the tropopause.

Wurtele, M. G.↗
NASA NTRS1988

Development and calibration of buried wire gages for wall shear stress measurements in fluid flow

Special methods were developed to arrange 'Buried Wire Gage' inserts flush to the contoured flow surfaces of instrument plugs of a boundary-layer flow apparatus. The fabrication process was aimed at producing proper bonding of the sensor wire to the substrate surface, without causing excessive surface waviness. A large number of gages were built and first calibrated for the resistance-temperature characteristics. The gages were then installed in a flow calibration apparatus and operated from a constant temperature anemometer system for a series of flow settings to derive the calibration constants of each of the gages. The flow settings included a range of subsonic freestream Mach numbers in order to help establish the gage calibration characteristics for compressible flow fields. This paper provides a description of the buried wire gage technique, an explanation of the method evolved for making proper gages, the procedure for calibrating the gages and the results of measurements performed for determining the calibration constants.

Murthy, Sreedhara V.↗
NASA NTRS1989

A laboratory model of severe downslope winds

Laboratory experiments are described in which flow visualization techniques were used to investigate the conditions under which a severe downslope wind may exist for linearly stratified flow over a ridge when the upstream flow is shear free. Three different ridges with similar cross sections but with maximum slopes of 40, 27 and 13 degrees were used. The results of the laboratory experiments are compared with previous numerical simulations and with a nonlinear hydrostatic theory for severe downslope winds.

Rottman, James W.↗
NASA NTRS1991

The stability to two-dimensional wakes and shear layers at high Mach numbers

This study is concerned with the stability properties of laminar free-shear-layer flows, and in particular symmetric two-dimensional wakes, for the supersonic through the hypersonic regimes. Emphasis is given to the use of proper wake profiles that satisfy the equations of motion at high Reynolds numbers. In particular the inviscid stability of a developing two-dimensional wake is studied as it accelerates at the trailing edge of a splitter plate. The nonparallelism of the flow is a leading-order effect in the calculation of the basic state, which is obtained numerically. Neutral stability characteristics are computed and the hypersonic stability is obtained by increasing the Mach number. It is found that the stability characteristics are altered significantly as the wake develops. Multiple modes (secondary modes) are found in the near wake that are closely related to the corresponding Blasius ones, but as the wake develops mode multiplicity is delayed to higher and higher Mach numbers. At a distance of about one plate length from the trailing edge, there is only one mode in a Mach number range of 0-20. The dominant mode emerging at all wake stations, and for high enough Mach numbers, is the so-called vorticity mode that is centered around the generalized inflection point layer. The structure of the dominant mode is also obtained analytically for all streamwise wake locations and it is shown how the far-wake limit is approached. Asymptotic results for the hypersonic mixing layer given by a tanh and a Lock distribution are also given.

Papageorgiou, Demetrios T.↗
NASA NTRS1990

Linear and non-linear studies of Alfven waves in space. Stationary and dynamic processes in magnetospheric plasmas

The Final Technical Report on linear and non-linear studies of Alfven waves in space is presented. Areas of research included relaxation of magnetotail plasmas with field-aligned currents; the equilibrium dayside magnetosphere; macroscale particle simulation of kinetic Alfven wave physics; ballooning stability of plasmas with sheared equilibrium flows; theory of the drift-mirror instability; collisionless tearing instability in magnetotail plasmas; and nonadiabatic behavior of the magnetic moment of a charged particle in a dipole magnetic field and the development of stochastic webs.

Bhattacharjee, A.↗