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At least 1,153 records · Page 64
All sourcesNASA NTRSDOE OSTI
DOE OSTI2024

CryoTRANS: predicting high-resolution maps of rare conformations from self-supervised trajectories in cryo-EM

Cryogenic electron microscopy (cryo-EM) has revolutionized structural biology, enabling efficient determination of structures at near-atomic resolutions. However, a common challenge arises from the severe imbalance among various conformations of vitrified particles, leading to low-resolution reconstructions in rare conformations due to a lack of particle images in these quasi-stable states. We introduce CryoTRANS, a method that predicts high-resolution maps of rare conformations by constructing a self-supervised pseudo-trajectory between density maps of varying resolutions. This trajectory is represented by an ordinary differential equation parameterized by a deep neural network, ensuring retention of detailed structures from high-resolution density maps. By leveraging a single high-resolution density map, CryoTRANS significantly improves the reconstruction of rare conformations and has been validated on four real-world datasets: alpha-2-macroglobulin, actin-binding protein complexes, SARS-CoV-2 spike glycoprotein, and the 70S ribosome. CryoTRANS can also predict high-resolution structures in cryogenic electron tomography maps using a high-resolution cryo-EM map.Cryogenic electron microscopy (cryo-EM) has revolutionized structural biology, enabling efficient determination of structures at near-atomic resolutions. However, a common challenge arises from the severe imbalance among various conformations of vitrified particles, leading to low-resolution reconstructions in rare conformations due to a lack of particle images in these quasi-stable states. We introduce CryoTRANS, a method that predicts high-resolution maps of rare conformations by constructing a self-supervised pseudo-trajectory between density maps of varying resolutions. This trajectory is represented by an ordinary differential equation parameterized by a deep neural network, ensuring retention of detailed structures from high-resolution density maps. By leveraging a single high-resolution density map, CryoTRANS significantly improves the reconstruction of rare conformations and has been validated on four real-world datasets: alpha-2-macroglobulin, actin-binding protein complexes, SARS-CoV-2 spike glycoprotein, and the 70S ribosome. CryoTRANS can also predict high-resolution structures in cryogenic electron tomography maps using a high-resolution cryo-EM map.

47 OTHER INSTRUMENTATION↗
DOE OSTI2021

Generalizing Similarity Laws for Radio-Frequency Discharge Plasmas across Nonlinear Transition Regimes

We generalize similarity theory based on the scaling and solution invariance of the Boltzmann equation, coupled with the Poisson equation, and demonstrate similarity laws for radio-frequency (rf) discharge plasmas across three nonlinear transitional regimes, namely, the alpha-gamma mode transition, the stochastic-Ohmic-heating mode transition, and the bounce-resonance-heating mode transition. Fundamental plasma parameters, e.g., the electron power absorption, under similar discharge conditions are examined via fully kinetic particle-in-cell simulations, and electron-kinetic invariance is exemplified in similar rf discharge plasmas. The results unambiguously confirm the applicability of similarity laws for rf plasmas in extended operating regimes, and strengthen the foundations and framework of similarity physics with universality.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗
NASA NTRS1975

Physical dosimetric evaluations in the Apollo 16 microbial response experiment

Texts of high-energy multicharged particle, ultraviolet light, and galactic irradiation fluxes on exposed biological specimens aboard Apollo 16 are described. Passive nuclear track detectors, passive dosimeters, and thermoluminescent dosimeters, respectively, were used for the measurements. Biological phenomena studied ranged from lipolytic alpha toxin production in Bacillus thuringiensis to animal tissue invasion (of human hair by Trichophyton terrestre). Fluxes observed were lower than those recorded by detectors located in the Biostack and in the passive personnel dosimeters worn by the astronauts, suggesting a greater average shielding in the latter two environments.

Taylor, G. R.↗
DOE OSTI2023

Selective thermal evolution of native oxide layer in Nb and Nb$_3$Sn-coated SRF grade Nb: An in-situ angular XPS study

This contribution discusses the results of an in-situ angular XPS study on the thermal evolution of the native oxide layer on Nb3Sn and pure Nb. XPS data were recorded with conventional spectrometers using an AlK(alpha) X-ray source for spectra collected up to 600 C, and an MgK(Alpha) X-rays source for temperatures above 600 C. The effect of the thickness, composition, and thermal stability of that oxide layer is relevant to understanding the functional properties of superconducting radiofrequency (SRF) cavities used in particle accelerators. There is a consensus that oxide plays a role in surface resistance (Rs). The focus of this study is Nb3Sn, which is a promising material that is used in the manufacturing of superconducting radiofrequency (SRF) cavities as well as in quantum sensing, and pure Nb, which was included in the study for comparison. The thermal evolution of the oxide layer in these two materials is found to be quite different, which is ascribed to the influence of the Sn atom on the reactivity of the Nb atom in Nb3Sn films. Nb and Sn atoms in this intermetallic solid have different electronegativity, and the Sn atom can reduce electron density around neighbouring Nb atoms in the solid, thus reducing their reactivity for oxygen. This is shown in the thickness, composition, and thermal stability of the oxide layer formed on Nb3Sn. The XPS spectra were complemented by grazing incident XRD patterns collected using the ESRF synchrotron radiation facility. The results discussed herein shed light on oxide evolution in the Nb3Sn compound and guide its processing for potential applications of the Nb3Sn-based SRF cavities in accelerators and other superconducting devices.

Cano, Arely↗
NASA NTRS1975

Angular distributions of electrons of energy E sub e greater than 0.06 MeV in the Jovian magnetosphere

The results of an angular distribution analysis of the electron intensity data recorded near Jupiter for the period from 26 November to 14 December 1973 are presented. The data were from three directional particle detectors with effective integral electron energy thresholds of 0.06, 0.55, and 5.0 Mev, respectively. It was found that the central core of the magnetosphere, within 12 Jupiter radii, is dominated by pitch angle distributions strongly peaked at alpha = 90 deg, while the region from 12 to 25 Jupiter radii shows bidirectional and approximately equal maxima at alpha = 0 and 180 deg. Bidirectional angular distributions in the magnetodisc out to the radius of the magnetopause strongly suggest quasi-trapping on closed field lines as the predominant situation. Substantial field aligned, unidirectional streaming was detected on only two occasions. No distinctive effects on angular distributions were discerned near the L-shells of satellites.

Sentman, D. D.↗
NASA NTRS1991

Simultaneous Solar Maximum Mission (SMM) and Very Large Array (VLA) observations of solar active regions

Very Large Array observations at 20 cm wavelength can detect the hot coronal plasma previously observed at soft x ray wavelengths. Thermal cyclotron line emission was detected at the apex of coronal loops where the magnetic field strength is relatively constant. Detailed comparison of simultaneous Solar Maximum Mission (SMM) Satellite and VLA data indicate that physical parameters such as electron temperature, electron density, and magnetic field strength can be obtained, but that some coronal loops remain invisible in either spectral domain. The unprecedent spatial resolution of the VLA at 20 cm wavelength showed that the precursor, impulsive, and post-flare components of solar bursts originate in nearby, but separate loops or systems of loops.. In some cases preburst heating and magnetic changes are observed from loops tens of minutes prior to the impulsive phase. Comparisons with soft x ray images and spectra and with hard x ray data specify the magnetic field strength and emission mechanism of flaring coronal loops. At the longer 91 cm wavelength, the VLA detected extensive emission interpreted as a hot 10(exp 5) K interface between cool, dense H alpha filaments and the surrounding hotter, rarefield corona. Observations at 91 cm also provide evidence for time-correlated bursts in active regions on opposite sides of the solar equator; they are attributed to flare triggering by relativistic particles that move along large-scale, otherwise-invisible, magnetic conduits that link active regions in opposite hemispheres of the Sun.

Willson, Robert F.↗
NASA NTRS1988

Reducing adhesion and agglomeration within a cloud of combustible particles

The study of combustible particle clouds inside flame tubes is of fundamental scientific interest as well as a practical concern. Only the suspended concentration is important to the combustion process, so that assurances must be provided that a minimum of particles adheres to the tube wall. This paper demonstrates experimentally the ability to minimize adhesion and agglomeration of acoustically-mixed lycopodium particles within a 5-cm diameter lexan flame tube. The area density of particles (ADP) adhering to the wall of bare lexan tubes was measured at greater than 100 particles/sq mm. The nature of adhesion was found to be clearly electrostatic, with the ADP level aggravated by increased mixing time, vigor, and the concentration of particles. Increases in the conductivity of the air and the tube wall did not affect ADP levels substantially. However, the observed adhesion was reduced to less than 10 p/sq mm when the air was ionized by use of an alpha emitter mounted on the inner walls of the flame tube.

Ross, Howard D.↗
NASA NTRS1992

A reanalysis of Voyager UVS observations of Titan

Data from the Voyager 1 Ultraviolet Spectrometer (UVS) encounter at Titan were reanalyzed to reveal new spatial and spectral information. The observations, data reduction and spectrum analysis are discussed. The following conclusions were drawn: Titan's airglow is driven by a combination of mechanisms including both EUV deposition and magnetospheric particle precipitation; the emissions are distributed throughout a range of altitudes in the atmosphere; enhanced nightside emissions of the Helium 58.4 nm line may be the result of a thermospheric diurnal temperature variation; the CD2 spectrum, with enhanced Ly(alpha) and N-2 64.5 and 67.0 nm emission, implies that some of the high altitude excitation is due to impact dissociative excitation of N2 by electrons with E greater than 150 eV; the CD2 spectrum is consistent with the existence of a high altitude bright spot in the dayside disk with dimensions from 400 to 900 km.

Hall, D. T.↗
NASA NTRS1994

Multispectral observations of chromospheric evaporation in the 1991 November 15 X-class solar flare

We analyze simultaneous H(alpha) images and spectra (from Mees Solar Observatory), and soft and hard X-ray images and spectra (from YOHKOH) during the early phase of an X1.5/3B flare. We investigate the morphological relationship between chromospheric downflows, coronal upflows, and particle precipitation sites, and the energetic relationship between conductive heating, nonthermal particle heating, and the chromospheric response. We find that the observations consistently fit the chromospheric evaporation model. In particular, we demonstrate that the observed upflowing coronal and downflowing chromospheric plasma components originate in the same locations, and we show that our unique set of optical and X-ray observations can clearly distinguish between conductively driven and electron beam driven evaporation.

Wulser, Jean-Pierre↗
DOE OSTI2022

$\mathrm{α}$ decay of the longest-lived Cm isotope: $^{247}_{96}\mathrm{Cm}$

We report alpha decay of 247 Cm was studied fifty years ago using a 1.6 Bq source containing 99.4 % 247 Cm and levels in 243 Pu were deduced. Using the same source, γ-ray spectra were measured with a more efficient Ge detector. A γ-ray spectrum was also measured in coincidence with α particles for a period of two months and has 80 times more counts than the previously published spectrum. Gamma-ray energies were determined with higher precision and four new γ rays were identified. A new high-energy γ ray of 564.5 keV was observed, which is interpreted as a transition between a level at 564.5 keV and the ground state of 243 Pu. The 564.5-keV level has been tentatively assigned to the 7/2 - [743] Nilsson state.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗
NASA NTRS1976

Angular distributions of electrons of energy greater than 0.06 MeV in the Jovian magnetosphere

Measurements of the angular distribution of electron intensity in the Jupiter magnetosphere were made by the University of Iowa experiment on Pioneer 10. The data were from three directional particle detectors with effective integral threshold of 0.06, 0.55 and 0.5 MeV respectively. It was discovered that the central core of the magnetosphere for radial distances less than 12 Jovian radii is dominated by pitch angle distributions strongly peaked at alpha = 90 degrees, while the region of radial distances 12-25 Jovian radii shows bidirectional and approximately equal maxima at alpha = 0 and 180 degrees. Substantial field-aligned streaming was detected on two occasions, each of about 1 hour duration. No distinctive effects on angular distributions were observed near the L shells of satellites.

Sentman, D. D.↗
NASA NTRS1995

Analysis of hydrogen Lyman-alpha observations of the coma of Comet P/Halley near the perihelion

The pioneer Venus Orbiter Ultraviolet Spectrometer measurements of the Lyman-alpha intensity of atomic hydrogen excited by solar resonance scattering in the coma of Comet P/Halley acquired from December 28, 1985, to January 6, 1986, and from January 31, 1986, to March 6, 1986, are simulated with the Monte Carlo Particle Trajectory Model corrected for optical depth effects. Spatially detailed comparisons between data and model show excellent agreement and are used to infer that the highest cometary activity may not be at perihelion, but about 2 1/2 weeks before. An improved set of H2O production rates is presented for the period of time that the spectrometer was observing and is found to be consistent with the rates from other types of measurements. The apparent discrepancy between Stewart (1987) in early March and International Ultraviolet Explorer OH derived rates is resolved. The problem with the conversion of 18-cm OH radio brightness to H2O production rates is also discussed.

Smyth, William H.↗
NASA NTRS1999

The Mars 2001 Athena Precursor Experiment (APEX)

The Athena Precursor Experiment (APEX) is a suite of scientific instruments for the Mars Surveyor Program 2001 (MSP'01) lander. The major elements of the APEX pay load are: (1) Pancam/Mini-TES, a combined stereo color imager and mid-infrared point spectrometer. (2) An Alpha-Proton-X-Ray Spectrometer (APXS) for in-situ elemental analysis. (3) A Mossbauer Spectrometer for in-situ determination of the mineralogy of Fe-bearing rocks and soils. (4) A Magnet Array that can separate magnetic soil particles from non-magnetic ones.

Squyres, S. W.↗
NASA NTRS1990

Voyager radio occultation by the Uranian rings: Structure, dynamics, and particle sizes

Diffraction of Voyager 2's 3.6 and 13 cm wavelength microwaves by the Uranian rings is removed through an inverse Fresnel transform filtering procedure that accommodates the significant eccentricity of the rings. Resulting 50 m resolution profiles at two observation longitudes: (1) reveal remarkably detailed and longitudinally varying structure, (2) provide eccentricity gradient profiles of Rings alpha, beta, and epsilon which bring into question current theoretical models for observed rigid precession, and (3) suggest that two possible unseen satellites may confine some of the very sharp edges observed via resonant interactions.

Gresh, Donna Leigh↗
NASA NTRS1980

Semiempirical models of chromospheric flare regions

Homogeneous plane-parallel semiempirical flare model atmospheres which reproduce observations in lines and continua of H I, Si I, C I, Ca II, and Mg II have a thin transition zone at the top of the enhanced chromosphere, indicating a significant amount of heating from the zone to the temperature minimum level. The minimum temperature is located deeper and is higher than in the quiet-sun and active-region models. The results do not agree with the particle-heated theoretical models, and it is suggested that the models of Brown (1973) and Henoux and Nakagawa (1977, 1978) do not include an essential term for heat conduction in their energy balance equations. It is concluded that substantial Ly-alpha radiative heating occurs in the upper chromosphere resulting from the conductive energy flux in the transition zone where the Ly-alpha line cools the gas.

Machado, M. E.↗
NASA NTRS1983

Recovery of damage in rad-hard MOS devices during and after irradiation by electrons, protons, alphas, and gamma rays

This paper reports on the recovery properties of rad-hard MOS devices during and after irradiation by electrons, protons, alphas, and gamma rays. The results indicated that complex recovery properties controlled the damage sensitivities of the tested parts. The results also indicated that damage sensitivities depended on dose rate, total dose, supply bias, gate bias, transistor type, radiation source, and particle energy. The complex nature of these dependencies make interpretation of LSI device performance in space (exposure to entire electron and proton spectra) difficult, if not impossible, without respective ground tests and analyses. Complete recovery of n-channel shifts was observed, in some cases within hours after irradiation, with equilibrium values of threshold voltages greater than their pre-irradiation values. This effect depended on total dose, radiation source, and gate bias during exposure. In contrast, the p-channel shifts recovered only 20 percent within 30 days after irradiation.

Brucker, G. J.↗
NASA NTRS1993

Comment on 'Comparison of observed and calculated implanted ion distributions outside Comet Halley's bow shock' by T. I. Gombosi, M. Neugebauer, A. D. Johnstone, A. J. Coates, and D. E. Huddleston

It is argued that the formulation used by Gombosi (1991) does not distinguish between two fundamental types of resonant interactions (those between cometary pickup ions and LF electromagnetic waves). Two problems arise from using their overly simplified expressions. The wave polarization of the solar wind Alfven wave is not purely right-handed, and values from alpha sub com and alpha sub sw are not well known, nor are the 'polarizations' of the 'backward'-going waves in either case. Gombosi et al. discuss the antisunward vs the sunward propagating solar wind Alfven wave, and assume a reasonable value of 0.2 for alpha sub sw. However, alpha sub com is totally unknown. It is concluded that a more correct analysis of wave polarization can lead to significant changes in the formulation and results from Gombosi et al. In his reply Gombosi argues that his simplified wave-particle interaction model is consistent with Giotto observations and plasma kinetic simulations and it leads to a reasonable approximation of the implanted ion transport coefficients. The more complicated approach suggested by Tsurutani & Thorne (1993) requires the introduction of several ad hoc parameters and it does not lead to any change of the energy diffusion coefficient.

Tsurutani, Bruce T.↗