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DOE OSTI2021

Assessment of Overmatched Filler (Alloy 617) to Improve Alloy 800H Stress Rupture Factors

Section III, Division 5 of the American Society of Mechanical Engineers (ASME) Boiler and Pressure Vessel Code (BPVC) specifies rules for elevated temperature nuclear reactors. Currently, only six metals are qualified in Section III, Division for construction of Class A metallic pressure boundary components for elevated-temperature service, one of these being Alloy 800H. There are only two permissible filler metals Alloy 800H is qualified to be welded with: Alloy A and Alloy 82. The stress rupture factors for these two filler metals are low near the maximum temperatures and service lives Alloy 800H is qualified for. This may preclude vendors from being able to use Alloy 800H for elevated-temperature nuclear construction. In this work, an overmatched filler metal, Alloy 617 is assessed to determine its potential to offer improved stress rupture factors. Scoping creep-rupture tests of cross-welds with Alloy 800H base metal and Alloy 617 filler metal were conducted. Preliminary results do not indicate that Alloy 617 will offer significantly improved stress rupture factors. Consequently, a matching filler metal, UTP A 2133, is now being investigated.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗

DOE OSTI2022

Constraints on the electron-hole pair creation energy and Fano factor below 150 eV from Compton scattering in a Skipper-CCD

Fully-depleted thick silicon Skipper-charge-coupled devices (Skipper-CCDs) have achieved sub-electron read-out noise and are an important technology to probe neutrino and light dark matter interactions. However, the successful search for rare neutrino or dark-matter events requires the signal and all backgrounds to be fully characterized. In particular, a measurement of the electron-hole pair creation energy below 150 eV and the Fano factor are necessary for characterizing the dark matter and neutrino signals. Moreover, photons from background radiation may Compton scatter in the silicon bulk, producing events that can mimic a dark matter or neutrino signal. We present a measurement of the Compton spectrum using a Skipper-CCD and a $^{241}$Am source. With these data, we measure the electron-hole pair-creation energy to be $\left(3.71 \pm 0.08\right)$ eV at 130 K in the energy range between 99.3 eV and 150 eV. By measuring the widths of the steps at 99.3 eV and 150 eV in the Compton spectrum, we introduce a novel technique to measure the Fano factor, setting an upper limit of 0.31 at 90% C.L. These results prove the potential of Skipper-CCDs to characterize the Compton spectrum and to measure precisely the Fano factor and electron-hole pair creation energy below 150 eV.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

DOE OSTI2022

Impact of Fill Factor on Electro-slag Remelting Operations

Vacuum induction melting (VIM), electro-slag remelting (ESR) and vacuum arc remelting (VAR) are all techniques used to make high quality ingots of complex chemistries. In some cases, all three are used, e.g., alloys for aerospace applications and increasingly those for fossil power applications under extreme conditions. Electroslag remelting operation is a complicated endeavor, dependent upon several factors including operating current, voltage, and geometry. A research scale 440 lb capacity ESR furnace was used to melt low carbon steel (1018 steel) electrodes of different diameters using various melt rates. This was used to assess melt parameters and sidewall and other quality aspects of the product ingots. These observations were compared to results from ESR modeling using MeltFlow-ESR. It was found that fill factor had a significant impact on operating voltage while current had a significant impact on melt rate. Ultimately, a combination of fill factor and power gave the best prediction of melt rate during steady state.

Jablonski, Paul↗

DOE OSTI2023

Neutron Magnetic Form Factor GNM Measurement at High Q2 with CLAS12

The neutron magnetic form factor, GnM , is a fundamental quantity that describes how magnetic properties are distributed within a neutron. This measurement provides insights into the internal structure of the neutron. Furthermore, by measuring GnM alongside three other form factors (GnE , GpE , GpM ), we can test the predictions of Quantum Chromodynamics (QCD), which is the fundamental theory governing the strong force that binds quarks and gluons. Additionally, these measurements play a significant role in constraining Generalized Parton Distributions (GPDs) and contribute to determining the angular momentum of the quarks within nucleons. The GnM was measured using the ratio of quasi-elastic e ? n to e ? p scattering from a deuteron target. The measurement covered a range of four-momentum transfer squared, Q2, from 5 to 12 GeV2, using three different beam energies (10.2, 10.4, and 10.6 GeV). The data is compared with previous measurements and several theoretical models. The preliminary results reveal that Gn M exceeds the predictions of the standard dipole parametrization by 12-20%, based on RG-B pass1 data. It is expected that this deviation from the standard dipole parametrization will diminish as we transition to using RG-B pass2 data, aligning GnM more closely with the dipole form factor. The ongoing RG-B pass2 analysis in CLAS12 holds considerable promise for significant improvement. This enhancement is achievable through collaborative efforts aimed at refining tracking efficiency, momentum correction, calibration, and alignment.

Baashen, Lamya↗

DOE OSTI2017

Integrating human factors issues into critical portable detection usage - 121

There are multiple situations where portable detection systems are used to quantify distributed radionuclides. Such applications include holdup measurements in decommissioning activities, other routine usage and safeguards applications. Human factors dominate equipment deployment and operation and have significant impact upon the resulting data collection and its quality. Equipment use includes simultaneous positioning and targeting the object/region being quantified, observation and reporting of incoming data, often in difficult conditions and often while dressed in protective equipment. We examine the human interface with the equipment and other human factors where balance, perception, situational awareness and personnel health level influence the physical control and positioning of the detector. All these aspects impact both the measurement outcome and the Total Measurement Uncertainty (TMU). In this work, we are developing approaches and solutions to: 1. Initiate an inclusive program to assess and evolve human factors aspects of the interface to provide real time feedback tailored to a specific field operator profile. 2. Develop a measurement system human interface on a 'bench top' detection system to allow for both audio and visual I/O for auditory attention and situational (objective and subjective) awareness across varied end user characteristics. 3. Assess and develop an enhanced health assessment plan to identify and monitor pertinent auditory and balance characteristics for operator(s) interface and physical control of the gamma detector. Use the plan to verify personnel ability for effective deployment. We take a multidisciplinary approach to maximize accuracy, efficiency, and safety for the end-user to optimize project and measurement performance. (authors)

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

DOE OSTI2017

Improved Modelling for the Assembly Discontinuity Factors in the APEC Method

Modern reactor analysis of light water reactor depends largely on a two-step procedure based on the simplified equivalence theory (SET). Consequently, the resulting homogenized two-group cross-section (XS) and associated discontinuity factor (DF) are subject to noticeable, intrinsic uncertainties. In our previous study, we introduced the albedo-corrected parameterized equivalence constants (APEC) method, which is a new leakage correction method applicable to the fuel assembly homogenized two-group XS of the standard two-step procedure. In the APEC method, fuel assembly two-group XS is expressed as a simple polynomial function of assembly-wise current-to-flux ratio (CFR), and it was demonstrated that two-group XSs can be well corrected during a nodal calculation and the nodal accuracy is improved a lot. However, since the conventional assembly discontinuity factor (ADF) is still used in the current APEC method, a small but non-negligible error is unavoidable. Therefore, it is necessary to include improved DF model in the APEC method for a higher accuracy. The functionalized discontinuity factor (FDF) using the definition of CFR was investigated by authors before the development of the APEC method, where it was motivated by the boundary perturbation theory by F. Rahnema. However, in our previous study on FDF, lattice calculations were performed only with one-dimensional two-group diffusion equation and could not be applied to actual core analysis. In this paper, feasibility study of DF functionalization is performed based on a 2-D method of characteristics (MOC) lattice code, DeCART2D.

21 SPECIFIC NUCLEAR REACTORS AND ASSOCIATED PLANTS↗

DOE OSTI2023

Performance and Commissioning of the BigBite Timing Hodoscope for Nucleon Form Factor Measurements at Jefferson Lab

The BigBite Timing Hodoscope detector is the primary subject of this thesis. The Super BigBite Spectrometer is a Jefferson Lab Hall A Collaboration project that has and will continue to measure nucleon electromagnetic form factors. This spectrometer includes the Timing Hodoscope which provides high resolution particle timing data for scattered electrons in the electron arm of BigBite. The Timing Hodoscope utilizes 90, 25 × 25 × 600 mm3 scintillator bars stacked on top of each other to form a single detector plane, and these bars are connected to 180 photo-multiplier tubes via light guides. Particles collide with the scintillating material creating a shower of optical photons and these particle events in the bars are collected to generate signals that are readout by the data acquisition (DAQ) electronics. NINO ASIC amplifier-discriminator cards output signals from the photo-multiplier tubes into analogue and logic signals, which are sent to analogue-to-digital (ADC) and time-to-digital (TDC) converter data acquisition readout modules. This data is then used for analysis of the detector. The focus of this thesis is the construction, commissioning, calibration, and performance of the BigBite Timing Hodoscope before and during the first of five nucleon electromagnetic form factor experiments at Jefferson Lab Hall A. Before the neutron magnetic form factor, G n M, experiment, cosmic ray data was collected during commissioning to confirm proper operation of the Timing Hodoscope electronics by observing the ADC and TDC data. Commissioning studies for charge normalization, gain matching, and other ADC and TDC detector data variables were performed before moving the detector into Hall A. Following installation in Hall A, several calibration studies were implemented to fine-tune the detector in preparation for use in the experiment. The calibration studies included analysis of timing cuts, TDC alignment, the time-walk effect, time difference offsets, and scintillator velocity corrections. Once the Timing Hodoscope was well-calibrated, data-taking during the experiment commenced and the beam-on-target data was used to characterize the Timing Hodoscope performance during the G n M experiment run-time. The performance analysis included studies observing energy deposit, cluster size, rates, accidentals, pile-up, tracking efficiency, position resolution, and time resolution. After application of physics cuts to ensure a data set comprised of particle tracks corresponding to elastic electrons, which is the main data of interest for measurement of G n M, the Timing Hodoscope is shown on average across all kinematic settings to have a >98% tracking efficiency, a position resolution of 4-6 cm in the non-dispersive plane and 1.5-2 cm in the dispersive plane, and a time resolution of 500-750 ps. These performance results are compared to a GEANT4 based performance simulation of the BigBite Timing Hodoscope for reference, showing to what degree the measured performance values match those taken from the simulation.

Marinaro, Ralph↗

DOE OSTI2024

Measurements of the Neutron Magnetic Form Factor and the Two-Photon Exchange Contribution to the Electron-Neutron Elastic Scattering Cross Section

The Super BigBite Spectrometer (SBS) program in Experimental Hall A of Jefferson Lab is a series of high-precision, large ?2 , electromagnetic form factor experiments. GMn and nTPE were the first two experiments of the SBS program and ran from Oc?tober 2021 through February 2022. Both experiments detect neutron-tagged ?(?, ???) and proton-tagged ?(?, ???) quasi-elastic electrons scattering from a deuterium tar?get. The GMn experiment is a measurement of the magnetic form factor of the neutron, ?? ?, via the ratio method over a ?2 range of 3.5 to 13.5 (GeV/c)2 . The nTPE experiment ran in-parallel with the GMn experiment and shared an overlap?ping kinematic point with GMn. nTPE extends the GMn experiment to assess the differences between Rosenbluth Slop and the single-photon exchange approximation. The nTPE experiment had two kinematic settings corresponding to different electron scatting angles at a fixed ?2 of 4.5 (GeV/c)? . Comparisons of nucleon form factor ratio measurements acquired through polarization transfer and LT separation reveal discrepancies as ?2 increases. Two-photon exchange is strongly favored as the reason for this discrepancy. ?? ? has been extracted at two kinematic settings corresponding to ?2 values of 3.0 and 4.5 (GeV/c)2 . Additionally, I will present an assessment of the differences between Rosenbluth Separation and single-photon exchange measure?ments.

Boyd III, John Alan↗

DOE OSTI2024

Geometry-aware training of factorized layers in tensor Tucker format

Reducing parameter redundancies in neural network architectures is crucial for achieving feasible computational and memory requirements during train and inference of large networks. Given its easy implementation and flexibility, one promising approach is layer factorization, which reshapes weight tensors into a matrix format and parameterizes it as the product of two rank-r matrices. However, this family of approaches often requires an initial full-model warm-up phase, prior knowledge of a feasible rank, and it is sensitive to parameter initialization.In this work, we introduce a novel approach to train the factors of a Tucker decomposition of the weight tensors. Our training proposal proves to be optimal in locally approximating the original unfactorized dynamics and stable for the initialization. Furthermore, the rank of each mode is dynamically updated during training.We provide a theoretical analysis of the algorithm, showing convergence, approximation and local descent guarantees. The method's performance is further illustrated through a variety of experiments, showing remarkable training compression rates and comparable or even better performance than the full baseline and alternative layer factorization strategies.

Zangrando, Emanuele [Gran Sasso Science Institute ↗

DOE OSTI2025

Precision Measurement of the Neutron Magnetic Form Factor via the Ratio Method at Jefferson Lab Hall A

Protons and neutrons, collectively known as nucleons, are composed of quarks and gluons. The Sachs electromagnetic form factors encode information about the spatial distributions of charge and magnetization in the nucleon, particularly at low momentum transfer. In particular, the neutron magnetic form factor (GMn) provides crucial information about the distribution of magnetization inside the neutron and helps constrain theoretical models of nucleon structure. Quasi-elastic electron scattering from deuterium was measured up to Q^2=13.5 GeV^2 using the Super BigBite Spectrometer in Hall A at Jefferson Lab. In this work, the neutron magnetic form factor GMn was extracted at Q^2 = 3.0 GeV^2 and Q^2=4.5 GeV^2 using the Ratio Method. These results represent a subset of the full dataset collected in this experiment, which extended to significantly higher Q^2. The extracted GMn values agree with the existing global fit within approximately two standard deviations at Q^2=3.0 and show excellent agreement at Q^2=4.5. The measurements achieved systematic uncertainties of about 2% and statistical uncertainties below 0.5%, among the most precise determinations of GMn at these kinematics. These results demonstrate the robustness of the experimental technique and provide an important validation point for future extractions at higher Q^2, where data remain scarce. In addition, the GRINCH heavy gas Cherenkov detector—a key component of the experimental apparatus—was commissioned and achieved an electron detection efficiency of approximately 97%, supporting reliable particle identification. Together, the analysis presented here advances both our understanding of nucleon structure and the validation of the experimental methods and instrumentation used to access it.

Satnik, Maria [College of William and Mary, Willia↗

DOE OSTI2016

Epidermal growth factor receptor mediated proliferation depends on increased lipid droplet density regulated via a negative regulatory loop with FOXO3/Sirtuin6

The proliferation of colon cancer cells is mediated in part by epidermal growth factor receptor (EGFR) signaling and requires sustained levels of cellular energy to meet its high metabolic needs. Intracellular lipid droplets (LDs) are a source of energy used for various cellular functions and they are elevated in density in human cancer, yet their regulation and function are not well understood. Here, in human colon cancer cells, EGF stimulates increases in LD density, which depends on EGFR expression and activation as well as the individual cellular capacity for lipid synthesis. Increases in LDs are blockaded by inhibition of PI3K/mTOR and PGE2 synthesis, supporting their dependency on select upstream pathways. In colon cancer cells, silencing of the FOXO3 transcription factor leads to down regulation of SIRT6, a negative regulator of lipid synthesis, and consequent increases in the LD coat protein PLIN2, revealing that increases in LDs depend on loss of FOXO3/SIRT6. Moreover, EGF stimulates loss of FOXO3/SIRT6, which is blockaded by the inhibition of upstream pathways as well as lipid synthesis, revealing existence of a negative regulatory loop between LDs and FOXO3/SIRT6. Elevated LDs are utilized by EGF treatment and their depletion through the inhibition of lipid synthesis or silencing of PLIN2 significantly attenuates proliferation. This novel mechanism of proliferative EGFR signaling leading to elevated LD density in colon cancer cells could potentially be therapeutically targeted for the treatment of tumor progression. - Highlights: • In colon cancer cells, EGFR activation leads to increases in LD density. • EGFR signaling includes PI3K/mTOR and PGE2 leading to lipid synthesis. • Increases in LDs are controlled by a negative regulatory loop with FOXO3/SIRT6. • EGFR mediated colon cancer cell proliferation depends on increased LD density.

60 APPLIED LIFE SCIENCES↗

DOE OSTI2018

Keratin 13 gene is epigenetically suppressed during transforming growth factor-β1-induced epithelial-mesenchymal transition in a human keratinocyte cell line

Highlights: • TGF-β1 induced mesenchymal-like morphology in HaCaT cells. • TGF-β1-induced EMT partially controlled the gene expression of EMT markers. • KRT13 was identified as a TGF-β1-responsive epithelial marker in HaCaT cells. • Chromatin state of the KRT13 promoter was epigenetically regulated during EMT. Epithelial-mesenchymal transition (EMT) is a biological event in which epithelial cells lose their polarity and cell–cell adhesions and concomitantly acquire mesenchymal traits, and is thought to play an important role in pathological processes such as wound healing and cancer progression. In this study, we evaluated transforming growth factor (TGF)-β1-treated human keratinocyte HaCaT cells as an in vitro model of EMT. HaCaT cells were changed into an elongated fibroblast-like morphology, which is indicative of EMT in response to TGF-β1. Phalloidin staining demonstrated the formation of actin stress fibers in TGF-β1-treated cells. Quantitative RT-PCR analysis revealed that TGF-β1 increased the mRNA levels of EMT transcription factors (SNAI2, TWIST1, and ZEB1) and mesenchymal markers (CDH2, VIM, and FN1), while it decreased the transcripts of epithelial phenotypic genes (CLDN1, OCLN, KRT5, KRT15, KRT13, and TGM1). Furthermore, we found that KRT13 was drastically suppressed through the reduction of RNA polymerase II occupancy of its promoter, which was accompanied by a decrease in active histone marks (H3K4me3 and H3K27ac) and an increase in a repressive mark (H3K27me3) during EMT. These findings indicate that the TGF-β1-induced EMT program regulates a subset of epithelial and mesenchymal marker genes, and that KRT13 is transcriptionally suppressed through the modulation of the chromatin state at the KRT13 promoter in HaCaT cells.

60 APPLIED LIFE SCIENCES↗

DOE OSTI2026

How threshold effects in spectroscopic factors influence heavy-ion knockout reactions

A two-decade-old puzzle in heavy-ion one-nucleon knockout reactions is the strong correlation between the reduction factor R S = σ exp /σ t h and the Fermi surface asymmetry ΔS. Theoretical cross sections typically rely on spectroscopic factors (SFs) from shell model (SM) calculations, which neglect continuum coupling effects. Here, we employ the Gamow shell model (GSM), which explicitly incorporates continuum coupling, to compute SFs for p-shell nuclei and predict corresponding theoretical cross sections. Systematic calculations demonstrate that using GSM-derived SFs substantially reduces discrepancies between theoretical and experimental results. This improvement is particularly significant for deeply bound nucleon knockout in nuclei near the dripline, where traditional SM-based calculations fall short. As a result, using GSM SFs, the ratio R s exhibits no pronounced dependence on ΔS. Furthermore, both the ratio of GSM SFs to SM SFs and their corresponding reaction cross sections ratios exhibit a strong ΔS dependence. We have also compared GSM SFs and cross sections with those from the no-core shell model calculations, giving a similar pronounced sensitivity to ΔS. Detailed analysis attributes these correlations to threshold effects for SFs in weakly bound systems. Overall, incorporating continuum coupling via GSM enhances the reliability of SF predictions for exotic, weakly bound nuclei and provides key insights toward resolving the enduring puzzle in heavy-ion knockout reactions from a nuclear structure perspective.

Gamow shell model↗

DOE OSTI2021

Tbx18-positive cells-derived myofibroblasts contribute to renal interstitial fibrosis via transforming growth factor-β signaling

It has been demonstrated that the T-box family transcription factor 18 (Tbx18) -positive cells give rise to renal mesenchymal cells and contribute to the development of the urinary system. However, it is unclear whether Tbx18-positive cells are the origin of the myofibroblasts during renal fibrosis. The present study aimed to determine the contribution of Tbx18-positive cells in kidney fibrosis and their underlying mechanism. We show that Tbx18-positive cells contribute to the development of the urinary system, especially renal fibroblasts. Following unilateral ureteral obstruction (UUO), genetic fate tracing results demonstrated that Tbx18-positive cells not only proliferate but also expand and differentiate into fibroblasts and myofibroblasts, indicating that they may act as profibrotic progenitors. Cell culture results suggest that transforming growth factor (TGF)-β promotes Tbx18-positive cells differentiation into myofibroblasts and assist their contribution to kidney fibrosis. Overall, the present study demonstrated that Tbx18-positive cells may act as profibrotic progenitor cells in a pathological condition of UUO-induced injury. Moreover, TGF-β may play a role in differentiation of Tbx18-positive cells into myofibroblasts in kidney fibrosis. These findings may provide a potential target on Tbx18-positive myofibroblast progenitors in the treatment of renal fibrosis.

60 APPLIED LIFE SCIENCES↗

DOE OSTI2023

Addressing Human and Organizational Factors in Nuclear Industry Modernization: A Sociotechnically Based Strategic Framework

The modernization of nuclear power plants will require an advanced concept of operations, involving an integrated set of tightly coupled systems in which all stakeholders act in a coordinated manner. For this modernization effort to be enabled, we developed a human and organizational factors approach based on a broad sociotechnical framework. Starting from core human factors principles, we conducted a literature review of the methods and approaches relevant to the modernization problem. These included not only core disciplines such as cognitive systems engineering, systems theoretic accident modeling and processes, human systems integration, resilience engineering, and macroergonomics but also related topics of safety culture and organizational change. From this literature, we developed a conceptual framework centered around the work system with its four interacting components: people, technology, process, and governance. In an effective work system, these four components are jointly optimized according to three systems criteria: efficiency, effectiveness, and safety. System failure may result from excessive emphasis on any one criterion. The actual work of attaining joint optimization in a given work system can be accomplished by utilizing three high-level functions: knowledge elicitation, knowledge representation, and cross-functional integration. Finally, we illustrated the utility of this approach by applying it to practical problems and case studies.

21 SPECIFIC NUCLEAR REACTORS AND ASSOCIATED PLANTS↗

DOE OSTI2024

Proton’s gluon GPDs at large skewness and gravitational form factors from near threshold heavy quarkonium photoproduction

We study the exclusive near threshold photoproduction of heavy quarkonium in the framework of the generalized parton distribution (GPD) factorization, taking the 𝐽/𝜓 production as an example. Because of the threshold kinematics, the Compton-like amplitudes are related to gluon GPDs at large skewness 𝜉, distinct from the common kinematics in asymptotic high energy where the skewness is typically small. We discuss the nature of large-𝜉 expansion of these amplitudes in terms of the moments of gluon GPDs in the large-𝜉 limit. Based on that, we propose several ways to extract the first few moments of the gluon GPDs from these amplitudes, with the leading ones corresponding to the gluonic gravitational or energy-momentum tensor form factors (GFFs). We apply these methods to analyze the recent near threshold 𝐽/𝜓 production measurements by the 𝐽/𝜓 007 experiment and GlueX Collaboration and find that the 𝜉 scaling of the measured differential cross sections is consistent with the asymptotic behavior. However, the current data are not accurate enough yet for a complete determination of the gluonic GFFs, and therefore we consider some prospects for better extractions in the future.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

DOE OSTI2025

Nonperturbative aspects of the electromagnetic pion form factor at high energies

The structure of hadronic form factors at high energies and their deviations from perturbative quantum chromodynamics provide insight on nonperturbative dynamics. Using an approach that is consistent with dispersion relations, we construct a model that simultaneously accounts for the pion wave function, gluonic exchanges, and quark Reggeization. In particular, we find that quark Reggeization can be investigated at high energies by studying scaling violation of the form factor.

Form factors↗

DOE OSTI2021

Spurious regulatory connections dictate the expression-fitness landscape of translation factors

During steady-state cell growth, individual enzymatic fluxes can be directly inferred from growth rate by mass conservation, but the inverse problem remains unsolved. Perturbing the flux and expression of a single enzyme could have pleiotropic effects that may or may not dominate the impact on cell fitness. Here, we quantitatively dissect the molecular and global responses to varied expression of translation termination factors (peptide release factors, RFs) in the bacterium Bacillus subtilis. While endogenous RF expression maximizes proliferation, deviations in expression lead to unexpected distal regulatory responses that dictate fitness reduction. Molecularly, RF depletion causes expression imbalance at specific operons, which activates master regulators and detrimentally overrides the transcriptome. Through these spurious connections, RF abundances are thus entrenched by focal points within the regulatory network, in one case located at a single stop codon. Such regulatory entrenchment suggests that predictive bottom-up models of expression-fitness landscapes will require near-exhaustive characterization of parts.

59 BASIC BIOLOGICAL SCIENCES↗