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NASA NTRS2020

Experimental Validation of Swarm Array Results Using Mars Cube One (MarCO) A,B Downlink Signals

In this paper, we investigate the feasibility of calibrating an orbiting phaased array composed of CubeSat class spacecraft together with a larger reference (or “chief”) spacecraft, comprising an overall Swarm Array. Considered as an analog to the Deep Space Network (DSN) Uplink Arraying problem [1], a spaceborne Swarm Array that can potentially deliver comparable or even greater operational performance than large monolithic spacecraft (such as increased datarates for telecommunications, or greater baselines for improved spatial resolution), thus increasing future mission capability but with significantly enhanced flexibility, evolvability and robustness.

Vilnrotter, Victor↗
NASA NTRS2020

An Efficient Focal Plane Alignment Methodology with Application to the ASTERIA Nanosatellite Space Telescope

The process of aligning an instrument focal plane toprecursor optics such as a telescope can be a difficult and timeconsuming process. The final alignment must achieve sufficientperformance at multiple locations across the field of view. Inthe case of instruments with infinite conjugates, such as a spacetelescope, there are practical challenges in illuminating the system at multiple off-axis field points simultaneously. Iterationduring the alignment process can cause a lengthy search for localminima within the performance cost function.This paper presents a focal plane alignment methodology thatavoids the above difficulties and obtains the desired level ofoptical performance in an efficient manner. The approach isbased on systematically measuring spot size across a set of fieldpoints and defocus positions, and then using a least squaresfit to determine the optimal location for the focal plane. Thisapproach has the additional benefit of revealing the amount offield curvature present in the instrument. It also provides theanalyst with a direct calculation of the shims needed to place thefocal plane at the computed best-fit location.This approach was successfully applied to the Arcsecond SpaceTelescope Enabling Research In Astrophysics (ASTERIA) payload. ASTERIA is a cubesat mission that was deployed intolow-Earth orbit in November 2017 and operated until December2019. In addition to presenting the theoretical basis for themethodology, this paper will present laboratory measurementsobtained during the payload alignment campaign. This approach is applicable to future space-borne optical instrumentsthat require an efficient methodology for focal plane alignmentwith limited cost or schedule resources.

Smith, Matthew W↗
NASA NTRS2020

An Inter Planetary Network Enabled by SmallSats

This paper describes the implementation of an unparalleled large sensor and communications platform along the solar system that we call the inter planetary network (IPN). The proposed IPN consists of thousands of small spacecraft (e.g. CubeSats) strategically deployed around planetary bodies in the solar system where each spacecraft is furnished with fast communications and sensor systems. The IPN concept is being proposed as an avant-garde science and communications platform that could allow for continuous fast communications and remarkable science returns. The IPN spacecraft, furnished with suitable miniaturized sensors, could form an amazing deep space platform for unique observation of the solar system, stars, galaxies and universe. A key feature of the IPN architecture is the use of swarms of spacecraft as network units. Super-high-speed intra-swarm communications could be achieved via omnidirectional optical links. The swarms act as autonomous network nodes and are capable of forming large synthetic apertures that enable high data rate communications among the IPN nodes. Depending on the sensors they carry, these swarms may also be capable of forming large synthetic sensors by rapidly combining data among the spacecraft. We envision distances between spacecraft forming a swarm to be in the range of 102-104 kilometers, whereas distances of 106-108 kilometers among swarms (IPN nodes) are expected. We provide an example of an initial IPN implementation in the inner solar system where swarms of spacecraft are deployed around the Earth, Moon, Mars, Venus and Mercury. Placing swarms around 3 Lagrange points per planet yield a total of 12 possible IPN network nodes. We present communications link calculations among IPN nodes that allow high data rate communications between Earth and Mars while Mars is in solar conjunction (behind the Sun). A second example of an IPN platforms includes a giant spaceborne radio telescope behind the moon.

Velazco, Jose E↗
NASA NTRS2020

Sample Transponder Implementations for PN DDOR

The design philosophy and recommended standards for PN DDOR transponders are proposed in RFM 20-04. The referenced document illustrates how to pick a chip rate, roll-off factor, and other PN parameters for a PN DDOR transmitter. The document also proposes various recommendations for these parameters. This document expands upon RFM 20-04 and presents two PN DDOR transmitters that were designed and tested at JPL. First the IRIS cubesat radio is presented with an 8 MHz spread spectrum DDOR signal. Next the Univeral Space Transponder (UST) transmitter is presented with a 32 MHz spread spectrum DDOR signal. These transmitter designs show that the proposed recommendations are reasonably implemented with current technology.

Volk, Christopher P↗
NASA NTRS2020

Snow and Water Imaging Spectrometer: Final Instrument Characterization

The Snow and Water Imaging Spectrometer (SWIS) is a science-grade imaging spectrometer and telescope system suitable for CubeSat applications, spanning a 350-1700 nm spectral range with 5.7 nm sampling, a 10 degree field of view and 0.3 mrad spatial resolution. The system operates at F/1.8, providing high throughput for low-reflectivity water surfaces, while avoiding saturation over bright snow or clouds. The SWIS design utilizes heritage from previously demonstrated instruments on airborne platforms, while advancing the state of the art in compact sensors of this kind in terms of size and spectral coverage. Through frequent repeat observations from space at a moderate spatial resolution, SWIS can address key science questions concerning aquatic and terrestrial ecosystem changes, cryosphere warming and melt behavior, cloud and atmospheric science, and potential impacts of climate change and human activities on the environment. We review the optical design and innovations and key technologies developed for this instrument, as well as its measured optical performance. We discuss the radiometric calibration characterization, including detector linearity, flat field correction, and SNR. Finally, we discuss stray light modeling and the development of a focused ghost removal algorithm, which is tested and supported by laboratory results.

Bellardo, John↗
NASA NTRS2020

MEXEC: An Onboard Integrated Planning and Execution Approach for Spacecraft Commanding

The traditional form of spacecraft commanding is with sequences that specify when commands should execute based on a schedule generated on the ground. Some sequences have control logic and event driven responses to increase flexibility, but it is limited. An approach to increase autonomy is to use goal-based planning and commanding. Using this paradigm, intention and behavior is modeled on board the spacecraft. In this paper we describe MEXEC (Multi-mission EXECutive), a multi-mission, task-based, onboard planning and execution software designed specifically to be used as flight software. As a path to infusion for future flight projects, we describe two experiments performed on the ASTERIA CubeSat and testbed that demonstrate that MEXEC can be integrated and used for spacecraft operations and increase robustness and science return compared to the standard sequences that were being used.

Campuzano, Brian↗
NASA NTRS2020

T2SL Focal Planes for Compact Remote Sensing Instruments

In this presentation, we will report our recent efforts in achieving high performance in Antimonides type-II superlattice (T2SL) based infrared photodetectors using the barrier infrared detector (BIRD) architecture. The initial BIRD devices (such as the nBn and the XBn) used either InAs absorber grown on InAs substrate, or lattice-matched InAsSb alloy grown on GaSb substrate, with cutoff wavelengths of ~3.2μm and ~4μm, respectively. While these detectors could operate at much higher temperatures than existing MWIR detectors based on InSb, their spectral responses do not cover the full (3 – 5.5μm) MWIR atmospheric transmission window. The T2SL BIRD devices not only covers the full MWIR atmospheric transmission window, but the full LWIR atmospheric transmission window and beyond. The LWIR detectors based on the BIRD architecture has also demonstrated significant operating temperature advantages over those based on traditional p-n junction designs. Two 6U SmalSat missions CIRAS (Cubesat Infrared Atmospheric Sounder) and HyTI (Hyperspectral Thermal Imager) are based on JPL’s T2SL BIRD focal plane arrays (FPAs). Based on III-V compound semiconductors, the BIRD FPAs offer a breakthrough solution for the realization of low cost (high yield), high-performance FPAs with excellent uniformity and pixel-to-pixel operability.

Ghuman, Parminder↗
NASA NTRS2020

Space Ground Sensorwebs for Volcano Monitoring

Increased space and ground sensing is enabling dramatic new measurements of a wide range of Earth Science and Applied Earth Science phenomena, including: volcanism, flooding, wildfires, weather, and many other phenomena. New Space ventures have produced significantly greater access to data and miniaturization of sensing has enabled cubesats and smallsats to deliver data of outstanding resolution. The advent of the internet of things has produced incredible amounts of relevant terrestrial data as well. Artificial Intelligence offers the potential to automate both data interpretation and resource allocation to best allocate sensing assets. We describe efforts to build and experiment with such “sensorweb” systems and offer some direction for the future sensorweb observation systems.

Zuleta, Ignacio↗
NASA NTRS2021

Validation of Small Satellite Dynamics Simulation Modules using ASTERIA Flight Data

ASTERIA (Arcsecond Space Telescope Enabling Research in Astrophysics) was a CubeSat space telescope that operated in low-Earth orbit, having been deployed from the International Space Station in 2017. The spacecraft has achieved sub-arcsecond pointing stability and millikelvin thermal stability over 20-minute observations. A key enabling feature of the ASTERIA mission is the low level of pointing error that has been demonstrated under ASTERIA’s fine pointing control mode. Prior to launch, the ASTERIA mission performed analysis and simulations to estimate the in-flight pointing performance. This analysis used reaction wheel models provided by JPL’s Small Satellite Dynamics Testbed (SSDT), which had performed Kistler table testing to characterize the jitter caused by the reaction wheels of the Blue Canyon Technologies XACT attitude control unit within ASTERIA. These models were subsequently incorporated into the SSDT simulation. The main source of jitter, the high-frequency attitude disturbance over the camera exposure time, on ASTERIA is the set of rotating reaction wheels that are used for maintaining fine pointing during observations. The reaction wheels impart disturbance forces and torques continuously that cause unwanted motion during imaging. Increased jitter consequently leads to blurring of the image. Therefore, to benefit all SmallSat missions devoted to photometric or spectroscopic astrophysics applications, an on-orbit flight data acquisition and jitter testing campaign was performed in an attempt to help validate the SSDT’s simulation models. This paper describes the process used to validate several simulation models and attempt to characterize the ASTERIA jitter by analyzing the size of the resulting spot size for the target stars as a function of wheel speed, having commanded four wheel speeds for each of three stars of known brightness. After summarizing the on-orbit observation and jitter level measurement process, this paper compares the results obtained from the flight operational environment to the corresponding set of simulation jitter levels. Though the flight data obtained during this experiment was not sufficient to validate the simulation’s jitter model, other models have been validated with flight data, including the magnetic field, orbit propagation, sun position, and long-duration orbit decay models, which may be used by all other projects that use the SSDT’s simulation to increase system capability knowledge.

Mohan, Swati↗
NASA NTRS2021

On-demand Command and Control of ASTERIA with Cloud-based Ground Station Services

ASTERIA (Arcsecond Space Telescope Enabling Research in Astrophysics) was a 6-unit CubeSat technology demonstration mission that deployed from the International Space Station on November 20th, 2017. After successfully completing its 90-day primary mission that demonstrated arcsecond-level line-of-sight pointing and focal plane thermal stability for exoplanet detection, it entered an extended mission performing onboard software demonstrations to mature technology both in space and on the ground. One of the technologies was a completely cloud-based ground system leveraging Amazon Web Services (AWS) Ground Station service.Announced in December 2018 and launched in May 2019, AWS Ground Station is a fully managed ground station service that aims to reduce the overhead associated with developing and maintaining ground system infrastructure throughout the mission lifecycle. AWS Ground Station makes available the suite of features required for any ground system in support of low-Earth orbit (LEO) and medium-Earth Orbit (MEO) satellite operations on-demand and without setting up or maintaining long-term contracts. Charges are incurred on a per-minute basis for antenna usage during scheduled tracks. Support is available for S-band uplink and downlink, along with X-band narrowband and wideband downlink. Missions that use the service may reserve tracks with any licensed AWS Ground Station antennas located across each service region and have direct access to any AWS services in support of mission operations.The cloud-based architecture built around the AWS Ground Station service greatly enhanced ASTERIA mission operations by enabling end-to-end pass automation, on-demand contact scheduling and contingency planning, along with more efficient data downlink through station availability and station-to-station handovers. It incorporated open-source software, particularly NASA's AMMOS Instrument Toolkit (AIT) and Open Mission Control Technologies (OpenMCT), along with the AWS application programming interfaces (API) to the Ground Station, Elastic Compute Cloud (EC2) and Simple Storage Service (S3) services. After showcasing operability in August 2019, the team continued using and improving this novel ground system architecture until the end of mission in December 2019. This paper describes the cloud-based ground system, how it was designed, tested, and evaluated with an in-orbit spacecraft, the operational capabilities that it enabled, along with lessons learned and recommendations for future missions.

Fesq, Lorraine↗
NASA NTRS2021

Demonstrations of System-Level Autonomy for Spacecraft

System-level autonomy refers to autonomously meeting the crosscutting needs of a system through awareness and coordinated control spanning the system's breadth of capabilities. In contrast to function-level autonomy, which focuses on capabilities required to achieve a specific function such as surface navigation or image recognition, system-level autonomy addresses the needs to coordinate and manage activities and resources, and estimate the state, across subsystems. This paper describes demonstrations that were conducted on a spacecraft workstation testbed. The autonomy was provided by system-level planning and execution integrated with system-level estimators of orbit knowledge and spacecraft hardware health. These components are embedded in a system-level framework defining how goals are formed and executed, which elements exist, and how control authority is distributed among components. The planning and execution system at the heart of the framework has the capability to schedule, execute and monitor completion of tasks, as well as plan around unexpected events including new science opportunities and anomalies. The planning and scheduling system is the Multi-mission EXECutive (MEXEC), supported by the system-level health state estimator Model-Based Off-Nominal State Identification and Detection (MONSID), and Autonomous Navigation (AutoNav) algorithms, which determine the orbital system state based on optical observation of other targets. These components are applicable to many kinds of missions on different platforms. These demonstrations were elaborations of earlier experiments conducted on the ASTERIA (Arcsecond Space Telescope Enabling Research In Astrophysics) CubeSat, described in a companion submission [1]. The spacecraft’s extended mission served as an in-flight test platform, during which some individual autonomous capabilities were flown successfully. The autonomy experiments described here were performed on the ASTERIA workstation testbed.

Prather, Maurice↗
NASA NTRSDate not supplied

Goniometric and Polarized Imaging Spectroscopic Lab Measurements ofSpacecraft Materials

To better characterize the spectral response of common spacecraft materials, the following laboratory measurements are presented to support the Space Situational Awareness community in the analysis of remotely sensed observational data. Of interest is classifying material reflective properties using spectral bidirectional reflectance distribution function (BRDF) data and spatially resolved polarized imaging spectroscopy, allowing laboratory data to be applicable to ground-based optical telescope observations. The team acquired a typical CubeSat solar panel and a sample of multi-layer insulation commonly used on spacecraft for initial measurements. The data were collected at the Goniometer of the Rochester Institute of Technology (GRIT) laboratory with a field and laboratory goniometer housing two Analytical Spectral Device (ASD) spectrometers and a Headwall micro-Hyperspec E-Series imaging spectrometer with an adjustable linear polarizer. The goniometer provides spectral reflectance over a broad spectral range from 350-2500 nm at 1 nm spacing with 3 nm spectral resolution in the visible and near infrared and 8 nm in the shortwave infrared. The Headwall imager covers a spectral range from 400-1000 nm with 1.6 nm spectral resolution. We present the results from these initial measurements that show highly reflective regions at various locations in the angular domain for both materials. In addition, the solar cell spectra exhibited strong interference effects typically observed with thin films. Our team is pursuing a variety of typical solar cells to assess variations in product type. The spatially resolved polarization ratio maps show variability across the materials due to surface structure and varying material composition. Based on these results, we outline a plan for simulating spectral radiance light curves of the materials in various orbital configurations as they would be measured from ground- based telescopes for a clear observing sky during twilight. The paper will also present a plan for expanding ours of interest to determine if the results presented are unique to these samples and to categorize the spectral response for different material classes.

Chris H. Lee↗
NASA NTRS2021

On-demand Command and Control of ASTERIA with Cloud-based Ground Station Services

ASTERIA (Arcsecond Space Telescope Enabling Research in Astrophysics) was a 6-unit CubeSat technology demonstration mission that deployed from the International Space Station on November 20th, 2017. After successfully completing its 90-day primary mission that demonstrated arcsecond-level line-of-sight pointing and focal plane thermal stability for exoplanet detection, it entered an extended mission performing onboard software demonstrations to mature technology both in space and on the ground. One of the technologies was a completely cloud-based ground system leveraging Amazon Web Services (AWS) Ground Station service. Announced in December 2018 and launched in May 2019, AWS Ground Station is a fully managed ground station service that aims to reduce the overhead associated with developing and maintaining ground system infrastructure throughout the mission lifecycle. AWS Ground Station makes available the suite of features required for any ground system in support of low-Earth orbit (LEO) and medium-Earth Orbit (MEO) satellite operations on-demand and without setting up or maintaining long-term contracts. Charges are incurred on a per-minute basis for antenna usage during scheduled tracks. Support is available for S-band uplink and downlink, along with X-band narrowband and wideband downlink. Missions that use the service may reserve tracks with any licensed AWS Ground Station antennas located across each service region and have direct access to any AWS services in support of mission operations. The cloud-based architecture built around the AWS Ground Station service greatly enhanced ASTERIA mission operations by enabling end-to-end pass automation, on-demand contact scheduling and contingency planning, along with more efficient data downlink through station availability and station-tostation handovers. It incorporated open-source software, particularly NASA's AMMOS Instrument Toolkit (AIT) and Open Mission Control Technologies (OpenMCT), along with the AWS application programming interfaces (API) to the Ground Station, Elastic Compute Cloud (EC2) and Simple Storage Service (S3) services. After showcasing operability in August 2019, the team continued using and improving this novel ground system architecture until the end of mission in December 2019. This paper describes the cloud-based ground system, how it was designed, tested, and evaluated with an inorbit spacecraft, the operational capabilities that it enabled, along with lessons learned and recommendations for future missions.

Fesq, Lorraine↗
NASA NTRS2021

Equuleus Launch Window Analysis and Mission Design

This paper presents the trajectory design process for EQUULEUS, a 6U CubeSat developed by JAXA and the University of Tokyo that is scheduled to launch as a piggyback of NASA’s Artemis 1. After separation from the upper stage of the Space Launch System, EQUULEUS will maneuver along a low-energy transfer to an Earth–Moon quasi-rectilinear halo orbit in 1-to-4 resonance with the lunar synodic period. As a secondary payload, the trajectory of EQUULEUS needs to be compatible with the requirements of the primary mission, but also robust against disturbances and potential changes in the deployment state. Realistic initial conditions spanning two years of potential launch windows are processed and the solution structure for optimal lunar transfers is analyzed. A host of candidate solutions is presented, compatibly with the fuel and power limitations of EQUULEUS. The global understanding of the solution space is shown to be insightful for the design of robust trajectories for limited control-authority spacecraft.

Kawakatsu, Yasuhiro↗
NASA NTRS2022

Compact Midwave Imaging System: Results from an Airborne Demonstration

The Compact Midwave Imaging System (CMIS) is a wide field of view, multi-angle, multi-spectral push frame imager that relies on the forward motion of the satellite to create a two-dimensional (2D) image swath. An airborne demonstration of CMIS was successfully completed in January-February 2021 on the NASA Langley Research Center Gulfstream III. The primary objective of the four-flight campaign was to demonstrate the capability of this unique instrument to perform stereo observations of clouds and other particulates (e.g. smoke) in the atmosphere. It is shown that the midwave infrared (MWIR) spectral bands of CMIS provide a unique 24/7 capability with high resolution for accurate stereo sensing.The instrument relies on new focal plane array (FPA) technology, which provides excellent sensitivity at much warmer detector temperatures than traditional technologies. This capability enabled a compact, low-cost design that can provide atmospheric motion vectors and cloud heights to support requirements for atmospheric winds in the 2017-2027 Earth Science Decadal Survey. Applications include day/night observations of the planetary boundary layer, severe weather, and wildfires. A comparison with current space-based earth science instruments demonstrates that the SWIR/MWIR multi-spectral capability of CMIS is competitive with larger, more expensive instrumentation. Imagery obtained over a controlled burn and operating nuclear power plant demonstrated the sensitivity of the instrument to temperature variations. The system relies on a mature stereoscopic imaging technique applied to the same scene from two independent platforms to unambiguously retrieve atmospheric motion vectors (AMVs) with accurate height assignment.This capability has been successfully applied to geostationary and low-earth orbit satellites to achieve excellent accuracy. When applied to a ground-point validation case, the accuracy for the CMIS aircraft observations was 20 m and 0.3 m/s for cloud heights and motion vectors, respectively. This result was confirmed by a detailed error analysis with analytical and covariance models. The results for CMIS cases with under flights of Aeolus, CALIPSO and Aqua provided a good validation of expected accuracies. The paper also showed the feasibility of accommodating CMIS on CubeSats to enable multiple instruments to be flown in a leader-follower mode.

stereo winds↗
NASA NTRS2021

T2SL Focal Planes for Compact Remote Sensing Instruments

In this presentation, we will report our recent efforts in achieving high performance in Antimonides type-II superlattice (T2SL) based infrared photodetectors using the barrier infrared detector (BIRD) architecture. The high operating temperature (HOT) BIRD focal plane arrays (FPAs) offer the same high performance, uniformity, operability, manufacturability, and affordability advantages as InSb. However, mid-wavelength infrared (MWIR) HOT-BIRD FPAs can operate at significantly higher temperatures (>150K) than InSb FPAs (typically 80K). Moreover, while InSb has a fixed cutoff wavelength (~5.4 µm), the HOT-BIRD offers a continuous adjustable cutoff wavelength, ranging from ~4 µm to >15 µm, and is therefore also suitable for long wavelength infrared (LWIR) as well. The LWIR detectors based on the BIRD architecture has also demonstrated significant operating temperature advantages over those based on traditional p-n junction designs. Two 6U SmalSat missions CIRAS (Cubesat Infrared Atmospheric Sounder) and HyTI (Hyperspectral Thermal Imager) are based on JPL’s T2SL BIRD focal plane arrays (FPAs). Based on III-V compound semiconductors, the BIRD FPAs offer a breakthrough solution for the realization of low cost (high yield), high-performance FPAs with excellent uniformity and pixel-to-pixel operability. We have also exploring the possibilities of implementing either metasurface resonator cavity or metasurface based flatlens to improve the signal-to-noise ratio of the detectors.

Ghuman, Parminder↗
NASA NTRS2021

High operating temperature T2SL digital focal plane arrays for earth remote sensing instruments

In this presentation, we will report our recent efforts in achieving high performance in Antimonides type-II superlattice (T2SL) based infrared photodetectors using the barrier infrared detector (BIRD) architecture. The high operating temperature (HOT) BIRD focal plane arrays (FPAs) offer the same high performance, uniformity, operability, manufacturability, and affordability advantages as InSb. However, mid-wavelength infrared (MWIR) HOT-BIRD FPAs can operate at significantly higher temperatures (>150K) than InSb FPAs (typically 80K). Moreover, while InSb has a fixed cutoff wavelength (~5.4 µm), the HOT-BIRD offers a continuous adjustable cutoff wavelength, ranging from ~4 µm to >15 µm, and is therefore also suitable for long wavelength infrared (LWIR) as well. The LWIR detectors based on the BIRD architecture has also demonstrated significant operating temperature advantages over those based on traditional p-n junction designs. Two 6U SmalSat missions CIRAS (Cubesat Infrared Atmospheric Sounder) and HyTI (Hyperspectral Thermal Imager) are based on JPL’s T2SL BIRD focal plane arrays (FPAs). Based on III-V compound semiconductors, the BIRD FPAs offer a breakthrough solution for the realization of low cost (high yield), high-performance FPAs with excellent uniformity and pixel-to-pixel operability. We have also exploring the possibilities of integrating either metasurface resonator cavity or metasurface based flatlens with individual pixels to improve the signal-to-noise ratio of the detectors. Furthermore, we will discuss the advantages of the utilization of all digital read out integrated circuits with HOT-BIRDs.

Ghuman, and Parminder↗
NASA NTRSDate not supplied

Investigating Biological Responses to Deep Space Radiation for Missions Beyond Low Earth Orbit (LEO) using Yeast

To enable long-term spaceflight missions and establish habitation on the Moon and Mars, we require a comprehensive understanding of the effects of chronic deep space radiation exposure on humans. BioSentinel is NASA’s first biological CubeSat to venture beyond Low Earth Orbit (LEO). It utilizes Saccharomyces cerevisiae (budding yeast) as a model organism to study biological responses to deep space radiation. Yeast share significant genetic homology with humans, including basic cellular metabolism and DNA repair mechanisms. In addition, unlike human cell cultures, yeast can survive the duration and constraints of a deep space mission. BioSentinel measures biological responses using an optical system and alamarBlue oxidation-reduction (redox) dye. Two strains of yeast are studied - a wild-type and a rad51 mutant strain that is deficient in DNA repair. Changes in metabolism and growth are monitored throughout the nominal 6-month mission. Preliminary tests indicate a significant change in the alamarBlue response to low-dose ionizing radiation (IR). Additionally, rad51 cells have shown an IR dose-dependent decrease in glucose uptake and accumulation of oxidized NADH (NAD+). These biomolecules are involved in reactions responsible for basic cell processes, including growth and development, signaling, and respiration. The current study expanded upon previous data by exposing yeast to deep space-relevant radiation. Glucose and NADH/NAD+ assays were conducted on yeast subjected to varying dosages of high-energy Fe-56 and simulated galactic cosmic rays (GCRs). The resulting data was analyzed using Excel and GraphPad Prism. A particular focus was to identify biomolecules resulting from aerobic respiration, which requires the presence of oxygen, or anaerobic processes. As long-term spaceflight missions draw near, it is increasingly important to characterize biological processes affected by the conditions of deep space. Studying biomolecular damage caused by deep space radiation may enable the development of engineering controls or biomedical therapeutics that mitigate health complications for future astronauts.

Kyra Keenan↗