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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 1,153 records · Page 64
All sourcesNASA NTRSDOE OSTI
DOE OSTI2015

BRIGHTEST X-RAY CLUSTERS OF GALAXIES IN THE CFHTLS WIDE FIELDS: CATALOG AND OPTICAL MASS ESTIMATOR

The Canada-France-Hawaii Telescope Legacy Survey (CFHTLS) presents a unique data set for weak-lensing studies, having high-quality imaging and deep multiband photometry. We have initiated an XMM-CFHTLS project to provide X-ray observations of the brightest X-ray-selected clusters within the wide CFHTLS area. Performance of these observations and the high quality of CFHTLS data allow us to revisit the identification of X-ray sources, introducing automated reproducible algorithms, based on the multicolor red sequence finder. We have also introduced a new optical mass proxy. We provide the calibration of the red sequence observed in the Canada-France-Hawaii filters and compare the results with the traditional single-color red sequence and photo-z. We test the identification algorithm on the subset of highly significant XMM clusters and identify 100% of the sample. We find that the integrated z-band luminosity of the red sequence galaxies correlates well with the X-ray luminosity, with a surprisingly small scatter of 0.20 dex. We further use the multicolor red sequence to reduce spurious detections in the full XMM and ROSAT All-Sky Survey (RASS) data sets, resulting in catalogs of 196 and 32 clusters, respectively. We made spectroscopic follow-up observations of some of these systems with HECTOSPEC and in combination with BOSS DR9 data. We also describe the modifications needed to the source detection algorithm in order to maintain high purity of extended sources in the shallow X-ray data. We also present the scaling relation between X-ray luminosity and velocity dispersion.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2015

Resolve survey photometry and volume-limited calibration of the photometric gas fractions technique

We present custom-processed ultraviolet, optical, and near-infrared photometry for the REsolved Spectroscopy of a Local VolumE (RESOLVE) survey, a volume-limited census of stellar, gas, and dynamical mass within two subvolumes of the nearby universe (RESOLVE-A and RESOLVE-B). RESOLVE is complete down to baryonic mass ∼10{sup 9.1−9.3} M{sub ⊙}, probing the upper end of the dwarf galaxy regime. In contrast to standard pipeline photometry (e.g., SDSS), our photometry uses optimal background subtraction, avoids suppressing color gradients, and employs multiple flux extrapolation routines to estimate systematic errors. With these improvements, we measure brighter magnitudes, larger radii, bluer colors, and a real increase in scatter around the red sequence. Combining stellar mass estimates based on our optimized photometry with the nearly complete H i mass census for RESOLVE-A, we create new z = 0 volume-limited calibrations of the photometric gas fractions (PGF) technique, which predicts gas-to-stellar mass ratios (G/S) from galaxy colors and optional additional parameters. We analyze G/S-color residuals versus potential third parameters, finding that axial ratio is the best independent and physically meaningful third parameter. We define a “modified color” from planar fits to G/S as a function of both color and axial ratio. In the complete galaxy population, upper limits on G/S bias linear and planar fits. We therefore model the entire PGF probability density field, enabling iterative statistical modeling of upper limits and prediction of full G/S probability distributions for individual galaxies. These distributions have two-component structure in the red color regime. Finally, we use the RESOLVE-B 21 cm census to test several PGF calibrations, finding that most systematically under- or overestimate gas masses, but the full probability density method performs well.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2022

Motivation, benefits, and challenges for new photovoltaic material & module developments

Abstract In the last decade and longer, photovoltaic module manufacturers have experienced a rapidly growing market along with a dramatic decrease in module prices. Such cost pressures have resulted in a drive to develop and implement new module designs, which either increase performance and/or lifetime of the modules or decrease the cost to produce them. In this paper, the main motivations and benefits but also challenges for material innovations will be discussed. Many of these innovations include the use of new and novel materials in place of more conventional materials or designs. As a result, modules are being produced and sold without a long-term understanding about the performance and reliability of these new materials. This has led to unexpected new failure mechanisms occurring few years after deployment, such as potential induced degradation or backsheet cracking. None of these failure modes have been detected after the back then common single stress tests. New accelerated test approaches are based on a combination or sequence of multiple stressors that better reflect outdoor conditions. That allows for identification of new degradation modes linked to new module materials or module designs.

Oreski, G. (ORCID:0000000342239047)↗
DOE OSTI2018

Metagenomic insights into diazotrophic communities across Arctic glacier forefields

Microbial nitrogen fixation is crucial for building labile nitrogen stocks and facilitating higher plant colonisation in oligotrophic glacier forefield soils. Here, the diazotrophic bacterial community structure across four Arctic glacier forefields was investigated using metagenomic analysis. In total, 70 soil metagenomes were used for taxonomic interpretation based on 185 nitrogenase (nif) sequences, extracted from assembled contigs. The low number of recovered genes highlights the need for deeper sequencing in some diverse samples, to uncover the complete microbial populations. A key group of forefield diazotrophs, found throughout the forefields, was identified using a nifH phylogeny, associated with nifH Cluster I and III. Sequences related most closely to groups including Alphaproteobacteria, Betaproteobacteria, Cyanobacteria and Firmicutes. Using multiple nif genes in a Last Common Ancestor analysis revealed a diverse range of diazotrophs across the forefields. Key organisms identified across the forefields included Nostoc, Geobacter, Polaromonas and Frankia . Nitrogen fixers that are symbiotic with plants were also identified, through the presence of root associated diazotrophs, which fix nitrogen in return for reduced carbon. Additional nitrogen fixers identified in forefield soils were metabolically diverse, including fermentative and sulphur cycling bacteria, halophiles and anaerobes.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2023

Antibacterial and antioxidant activities of Streptomyces sp. strain FR7 isolated from forest soil

Abstract Actinomycetes produce secondary metabolites with many bioactivities such as antimicrobial, which can be useful as alternatives against resistant bacterial strains. Therefore, the screening of new habitats is likely to provide new strains with high potential. In this work, the antimicrobial capacity was used to select Streptomyces sp. strains isolated from Raf Raf forest (Tunisia). From the strain displaying higher activity, FR7, an ethyl acetate extract was prepared under optimized culturing conditions (10 days at 30°C in ISP2 medium with initial pH 8), showing significant antimicrobial activity against Micrococcus luteus and Staphylococcus aureus (MIC = 5 μg ml−1), and Listeria monocytogenes and Pseudomonas aeruginosa (MIC = 20 μg ml−1). The extract displayed strong DPPH radical scavenging activity (IC50 = 1.3 μg ml−1) and protection of yeast cells from H2O2-induced oxidative stress determined by flow cytometry with dichlorofluorescein diacetate. The crude extract showed the presence of polyketides, with methylsalicylic acid as moiety, a large and diverse group of secondary metabolites with a wide range of bioactivities, including antioxidant and antibacterial. Based on 16S RNA gene sequences, strain FR7 was identified as belonging to genus Streptomyces with high resemblance to S. iakyrus. Streptomyces sp. FR7 has great potential as a source of antibacterial and antioxidant metabolites.

Weslati, Imen↗
DOE OSTI2022

Translation and rotation equivariant normalizing flow (TRENF) for optimal cosmological analysis

ABSTRACT Our Universe is homogeneous and isotropic, and its perturbations obey translation and rotation symmetry. In this work, we develop translation and rotation equivariant normalizing flow (TRENF), a generative normalizing flow (NF) model which explicitly incorporates these symmetries, defining the data likelihood via a sequence of Fourier space-based convolutions and pixel-wise non-linear transforms. TRENF gives direct access to the high dimensional data likelihood p(x|y) as a function of the labels y, such as cosmological parameters. In contrast to traditional analyses based on summary statistics, the NF approach has no loss of information since it preserves the full dimensionality of the data. On Gaussian random fields, the TRENF likelihood agrees well with the analytical expression and saturates the Fisher information content in the labels y. On non-linear cosmological overdensity fields from N-body simulations, TRENF leads to significant improvements in constraining power over the standard power spectrum summary statistic. TRENF is also a generative model of the data, and we show that TRENF samples agree well with the N-body simulations it trained on, and that the inverse mapping of the data agrees well with a Gaussian white noise both visually and on various summary statistics: when this is perfectly achieved the resulting p(x|y) likelihood analysis becomes optimal. Finally, we develop a generalization of this model that can handle effects that break the symmetry of the data, such as the survey mask, which enables likelihood analysis on data without periodic boundaries.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2016

Intrinsic alignments in redMaPPer clusters – I. Central galaxy alignments and angular segregation of satellites

The shapes of cluster central galaxies are not randomly oriented, but rather exhibit coherent alignments with the shapes of their parent clusters as well as with the surrounding large-scale structures. In this work, we aim to identify the galaxy and cluster quantities that most strongly predict the central galaxy alignment phenomenon among a large parameter space with a sample of 8237 clusters and 94 817 members within 0.1 < z < 0.35, based on the red-sequence Matched-filter Probabilistic Percolation cluster catalogue constructed from the Sloan Digital Sky Survey. We first quantify the alignment between the projected central galaxy shapes and the distribution of member satellites, to understand what central galaxy and cluster properties most strongly correlate with these alignments. Next, we investigate the angular segregation of satellites with respect to their central galaxy major axis directions, to identify the satellite properties that most strongly predict their angular segregation. We find that central galaxies are more aligned with their member galaxy distributions in clusters that are more elongated and have higher richness, and for central galaxies with larger physical size, higher luminosity and centring probability, and redder colour. Satellites with redder colour, higher luminosity, located closer to the central galaxy, and with smaller ellipticity show a stronger angular segregation towards their central galaxy major axes. Lastly, we provide physical explanations for some of the identified correlations, and discuss the connection to theories of central galaxy alignments, the impact of primordial alignments with tidal fields, and the importance of anisotropic accretion.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2018

Polynucleobacter meluiroseus sp. nov., a bacterium isolated from a lake located in the mountains of the Mediterranean island of Corsica

Strain AP-Melu-1000-B4 was isolated from a lake located in the mountains of the Mediterranean island of Corsica (France). Phenotypic, chemotaxonomic and genomic traits were investigated. Phylogenetic analyses based on 16S rRNA gene sequencing referred the strain to the cryptic species complex PnecC within the genus Polynucleobacter . The strain encoded genes for biosynthesis of proteorhodopsin and retinal. When pelleted by centrifugation the strain showed an intense rose colouring. Major fatty acids were C 16:1 ω7 c , C 16:0 , C 18:1 ω7 c and summed feature 2 (C 16:1 isoI and C 14:0 - 3OH). The sequence of the 16S rRNA gene contained an indel which was not present in any previously described Polynucleobacter species. Genome sequencing revealed a genome size of 1.89 Mbp and a G+C content of 46.6 mol%. In order to resolve the phylogenetic position of the new strain within subcluster PnecC, its phylogeny was reconstructed from sequences of 319 shared genes. To represent all currently described Polynucleobacter species by whole genome sequences, three type strains were additionally sequenced. Our phylogenetic analysis revealed that strain AP-Melu-100-B4 occupied a basal position compared with previously described PnecC strains. Pairwise determined whole genome average nucleotide identity (gANI) values suggested that strain AP-Melu-1000-B4 represents a new species, for which we propose the name Polynucleobacter meluiroseus sp. nov. with the type strain AP-Melu-1000-B4 T (=DSM 103591 T =CIP 111329 T )

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2023

Sphingobium lignivorans sp. nov., isolated from river sediment downstream of a paper mill

Here, a bacterial isolate, B1D3A T , was isolated from river sediment collected from the Hiwassee River near Calhoun, TN, by enrichment culturing with a model 5–5' lignin dimer, dehydrodivanillate, as its sole carbon source. B1D3A T was also shown to utilize several model lignin-derived monomers and dimers as sole carbon sources in a variety of minimal media. Cells were Gram-stain-negative, aerobic, motile, rod-shaped and formed yellow/cream-coloured colonies on rich agar. Optimal growth occurred at 30°C, pH 7–8, and in the absence of NaCl. The major fatty acids of B1D3A T were C 18:1 ω7c and C 17:1 ω6c. The predominant hydroxy fatty acids were C 14: 0 2-OH and C 15:0 2-OH. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidyldimethylethanolamine and sphingoglycolipid. B1D3A T contained spermidine as the only major polyamine. The major isoprenoid quinone was Q-10 with minor amounts of Q-9 and Q-11. The genomic DNA G+C content of B1D3A T was 65.6mol%. Phylogenetic analyses based on 16S rRNA gene sequences and coding sequences of 49 core, universal genes defined by Clusters of Orthologous Groups gene families indicated that B1D3A T was a member of the genus Sphingobium. B1D3A T was most closely related to Sphingobium sp. SYK-6, with a 100% 16S rRNA gene sequence similarity. B1D3A T showed 78.1–89.9%average nucleotide identity and 19.5–22.2% digital DNA–DNA hybridization identity with other type strains from the genus Sphingobium. On the basis of phenotypic and genotypic properties and phylogenetic inference, strain B1D3A T should be classified as representing a novel species of the genus Sphingobium, for which the name Sphingobium lignivorans sp. nov. is proposed. The type strain is strain B1D3A T (ATCC TSD-279 T =DSM 111877 T ).

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2018

Genome-reconstruction for eukaryotes from complex natural microbial communities

Microbial eukaryotes are integral components of natural microbial communities, and their inclusion is critical for many ecosystem studies, yet the majority of published metagenome analyses ignore eukaryotes. In order to include eukaryotes in environmental studies, we propose a method to recover eukaryotic genomes from complex metagenomic samples. A key step for genome recovery is separation of eukaryotic and prokaryotic fragments. We developed a k-mer-based strategy, EukRep, for eukaryotic sequence identification and applied it to environmental samples to show that it enables genome recovery, genome completeness evaluation, and prediction of metabolic potential. We used this approach to test the effect of addition of organic carbon on a geyser-associated microbial community and detected a substantial change of the community metabolism, with selection against almost all candidate phyla bacteria and archaea and for eukaryotes. Near complete genomes were reconstructed for three fungi placed within the Eurotiomycetes and an arthropod. While carbon fixation and sulfur oxidation were important functions in the geyser community prior to carbon addition, the organic carbon-impacted community showed enrichment for secreted proteases, secreted lipases, cellulose targeting CAZymes, and methanol oxidation. We demonstrate the broader utility of EukRep by reconstructing and evaluating relatively high-quality fungal, protist, and rotifer genomes from complex environmental samples. This approach opens the way for cultivation-independent analyses of whole microbial communities.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2023

An Admittance-Based Protection Scheme for Microgrids

This paper presents a novel directional protection scheme to address the unique challenges associated with protection during unbalanced faults in a distribution system with high penetration of inverter-based resources. The scheme is based on a superimposed negative sequence admittance method. A coordination approach using a unique inverse-time undervoltage element is also presented. An analysis of the scheme, the detection criteria and the coordination method are performed. Also the impact of inverter-control on the scheme is discussed. Results from extensive simulation using MATLAB/Simulink are presented to show a selective and reliable response of the scheme to unbalanced faults

Opoku, Kwasi↗
DOE OSTI2020

Impacts of Ternary-Pumped Storage Hydropower on U.S. Western Interconenction with Extremely High Renewable Penetrations

In recent years, ternary pumped storage hydropower (T-PSH), as one of the advanced PSH technologies, has attracted more attention from the industry. The interest is because of the capability of providing fast power support for the future electric grid with high penetration of renewable energy (RE). This paper focuses on studying the impact of T-PSH on frequency response of the U.S. Western Interconnection under different penetration levels of RE, which are from 20% to 80%. The detailed model of T-PSH is developed and implemented in the Western Interconnection grid model based on the GE Positive Sequence Load Flow (PSLF) platform, which can capture the unique dynamics of hydraulic short-circuit (HSC) mode and fast mode switching of T-PSH. To reveal the dynamic benefit from T-PSH, the frequency response of the Western Interconnection has been compared with and without T-PSH. In addition, a new solution is discussed that uses T-PSH to improve system stability issues caused by high RE penetration.

13 HYDRO ENERGY↗
DOE OSTI2022

Extension of the Generic Multi-Frequency Modelling Method for Type 3 Wind Turbines

Reflecting potential non-linearities of converter-based systems, especially frequency and sequence couplings, is an ongoing challenge for linearized multi-frequency models. Besides, design details are required to develop such models, which either are the intellectual property of manufacturers or require experimental tests. The generic multi-frequency modelling method has been proposed to fill this gap; however, it is only developed for converter-connected systems, e.g., Type 4 Wind Turbines (WT). This paper proposes to extend the application of the generic multi-frequency modelling method for Type 3 WTs. In this way, a theory for patterns of the couplings in Type 3 WTs is proposed. Accordingly, a group of emissions and couplings are Rotor-Speed-Dependent (RSD). The RSD emissions and couplings are particular characteristics of Type 3 WTs, which should be addressed in the generic multi-frequency models. Additionally, the proposed theory is verified by unique-worldwide experimental perturbation tests on a 2 MVA Type 3 WT using a 7 MVA grid emulator. Accordingly, a limited number of RSD couplings and emissions are observed in the test results, mainly in low frequencies (below 1 kHz). Therefore, addressing the RSD couplings is practical and important to extend the generic multi-frequency modelling for Type 3 WTs.

24 POWER TRANSMISSION AND DISTRIBUTION↗
DOE OSTI2024

Testing of a Line Driver With Configurable Pre-Emphasis on Lossy Transmission Lines

Rare-event physics experiments such as the Deep Underground Neutrino Experiment (DUNE) or the next Enriched Xenon Observatory (nEXO) experiment search for rare, low-energy events, detected by sensitive detectors immersed in a cryogenic noble liquid (e.g., liquid argon or xenon). Readout electronics used within such detectors must consume minimal power while operating reliably in cryogenic environments. Furthermore, in the case of nEXO, maximizing the radiopurity of the environment is vital to minimize background noise, thus placing strict limits on the volume of dielectric materials, leading to high-loss data cables spanning distances up to 12 m. Such cables cause high attenuation and intersymbol interference (ISI), resulting in a high bit-error rate (BER). These issues were addressed by developing an integrated line driver with configurable pre-emphasis in a 65-nm CMOS process. The pre-emphasis parameters can be programmed to minimize BER for specific cables and data rates under power constraints. Here, the driver was tested at both room and cryogenic temperatures. In both cases, the output BER was found to be strongly correlated with the pre-emphasis settings. Furthermore, analysis and simulation showed that adapting the pre-emphasis settings based on the incoming bit sequence can further improve performance with minimal changes to the current solution.

47 OTHER INSTRUMENTATION↗
DOE OSTI2015

Regulation of Gene Expression during the Onset of Ligninolytic Oxidation by Phanerochaete chrysosporium on Spruce Wood

Since uncertainty remains about how white rot fungi oxidize and degrade lignin in wood, it would be useful to monitor changes in fungal gene expression during the onset of ligninolysis on a natural substrate. In this paper, we grew Phanerochaete chrysosporium on solid spruce wood and included oxidant-sensing beads bearing the fluorometric dye BODIPY 581/591 in the cultures. Confocal fluorescence microscopy of the beads showed that extracellular oxidation commenced 2 to 3 days after inoculation, coincident with cessation of fungal growth. Whole transcriptome shotgun sequencing (RNA-seq) analyses based on the v.2.2 P. chrysosporium genome identified 356 genes whose transcripts accumulated to relatively high levels at 96 h and were at least four times the levels found at 40 h. Transcripts encoding some lignin peroxidases, manganese peroxidases, and auxiliary enzymes thought to support their activity showed marked apparent upregulation. The data were also consistent with the production of ligninolytic extracellular reactive oxygen species by the action of manganese peroxidase-catalyzed lipid peroxidation, cellobiose dehydrogenase-catalyzed Fe 3+ reduction, and oxidase-catalyzed H 2 O 2 production, but the data do not support a role for iron-chelating glycopeptides. In addition, transcripts encoding a variety of proteins with possible roles in lignin fragment uptake and processing, including 27 likely transporters and 18 cytochrome P450s, became more abundant after the onset of extracellular oxidation. Genes encoding cellulases showed little apparent upregulation and thus may be expressed constitutively. Finally, transcripts corresponding to 165 genes of unknown function accumulated more than 4-fold after oxidation commenced, and some of them may merit investigation as possible contributors to ligninolysis.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2024

The protein structurome of Orthornavirae and its dark matter

Metatranscriptomics is uncovering more and more diverse families of viruses with RNA genomes comprising the viral kingdom Orthornavirae in the realm Riboviria. Thorough protein annotation and comparison are essential to get insights into the functions of viral proteins and virus evolution. In addition to sequence- and hmm profile-based methods, protein structure comparison adds a powerful tool to uncover protein functions and relationships. We constructed an Orthornavirae “structurome” consisting of already annotated as well as unannotated (“dark matter”) proteins and domains encoded in viral genomes. We used protein structure modeling and similarity searches to illuminate the remaining dark matter in hundreds of thousands of orthornavirus genomes. The vast majority of the dark matter domains showed either “generic” folds, such as single α-helices, or no high confidence structure predictions. Nevertheless, a variety of lineage-specific globular domains that were new either to orthornaviruses in general or to particular virus families were identified within the proteomic dark matter of orthornaviruses, including several predicted nucleic acid-binding domains and nucleases. In addition, we identified a case of exaptation of a cellular nucleoside monophosphate kinase as an RNA-binding protein in several virus families. Notwithstanding the continuing discovery of numerous orthornaviruses, it appears that all the protein domains conserved in large groups of viruses have already been identified. The rest of the viral proteome seems to be dominated by poorly structured domains including intrinsically disordered ones that likely mediate specific virus-host interactions.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2019

Recent Advances in Understanding the Role of Genomic and Epigenomic Factors in Noncommunicable Diseases

The NGS based whole genome and exome sequencing endeavors have identified a plethora of mutations in the coding regions of the human genome that are held responsible as the causative factors for various human noncommunicable diseases including cancer. These mutations encompass various nonsynonymous nucleotide substitutions causing missense, nonsense, and frameshift changes in the protein-coding genes. For these types of mutations it is easier to establish a causal link between these mutations and disease phenotypes as they tend to change the amino acids in the proteins resulting in their loss-of-function (of tumor suppressor genes) or gain-of-function (of oncogenes) which might be directly responsible for the disease such as cancer. Apart from these disease-causing mutations, vast majority (more than 80%) of single-nucleotide polymorphisms were distributed throughout the human genome, mostly in the noncoding regions such as introns and intergenic regions. Large body of evidence suggested that variants in the noncoding regions can be considered as genetic predisposition to many noncommunicable diseases including cancer.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2018

Protein Coverage Summarizer

The Protein Coverage Summarizer can be used to determine the percent of the residues in each protein sequence that have been identified, based on a list of identified peptides. A graphical user interface (GUI) is provided to allow the user to select the input files, set the options, and browse the coverage results.

Gibbons, Bryson↗