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At least 1,153 records · Page 64

Shoreline as a controlling factor in commercial shrimp production

An ecological model was developed that relates marsh detritus export and shrimp production, based on the hypothesis that the shoreline is a controlling factor in the production of shrimp through regulation of detritus export from the marsh. LANDSAT data were used to develop measurements of shoreline length and area of marsh having more than 5.0 km shoreline/sq km for the coast of Louisiana, demonstrating the capability of remote sensing to provide important geographic information. These factors were combined with published tidal ranges and salinities to develop a mathematical model that predicted shrimp production for nine geographic units of the Louisiana coast, as indicated by the long term average commercial shrimp yield. The mathematical model relating these parameters and the shrimp production is consistent with an energy flow model describing the interaction of detritus producing marshlands with shrimp nursery grounds and inshore shrimping areas. The analysis supports the basic hypothesis and further raises the possibility of applications to coastal zone management requirements.

Faller, K. H.↗

Development of V/STOL methodology based on a higher order panel method

The development of a computational technique to predict the complex flowfields of V/STOL aircraft was initiated in which a number of modules and a potential flow aerodynamic code were combined in a comprehensive computer program. The modules were developed in a building-block approach to assist the user in preparing the geometric input and to compute parameters needed to simulate certain flow phenomena that cannot be handled directly within a potential flow code. The PAN AIR aerodynamic code, which is higher order panel method, forms the nucleus of this program. PAN AIR's extensive capability for allowing generalized boundary conditions allows the modules to interact with the aerodynamic code through the input and output files, thereby requiring no changes to the basic code and easy replacement of updated modules.

Bhateley, I. C.↗

Foam flotation as a separation process

The basic principles of foam separation techniques are discussed. A review of the research concerning bubble-particle interaction and its role in the kinetics of the flotation process is given. Most of the research in this area deals with the use of theoretical models to predict the effects of bubble and particle sizes, of liquid flow, and of various forces on the aperture and retention of particles by bubbles. A discussion of fluid mechanical aspects of particle flotation is given.

Currin, B. L.↗

The relationship between proton temperature and momentum flux density in the solar wind

The relationship between proton temperature and momentum flux density/unit mass at 1 AU is examined using Helios 1 solar wind data from 1974 to 1980. In high-speed plasma (V greater than 500 km/s) T(p) increases with increasing n(0) V-squared, where n(0) and T(p) are the density and proton temperature at 1 AU and V is the flow speed. In lowspeed plasma (V less than 500 km/s), T(p) does not increase with increasing n(0) V-squared, and perhaps tends to decrease slightly. These basic relationships between T(p) and n(0) V-squared are not significantly affected by stream interactions. A qualitative explanation of these results is offered in the context of a solar wind model that includes deposition of momentum and energy extending well outward into the interplanetary medium.

Lopez, R. E.↗

The plasma physics of shock acceleration

The history and theory of shock acceleration is reviewed, paying particular attention to theories of parallel shocks which include the backreaction of accelerated particles on the shock structure. The work that computer simulations, both plasma and Monte Carlo, are playing in revealing how thermal ions interact with shocks and how particle acceleration appears to be an inevitable and necessary part of the basic plasma physics that governs collisionless shocks is discussed. Some of the outstanding problems that still confront theorists and observers in this field are described.

Jones, Frank C.↗

High-resolution imaging of QSO 2305 + 187 (= 4C 18.68)

Optical imaging of QSO 2305 + 187 with resolution ranging from 0.1 to 1.0 arcsec in continuum and line emission is presented. The data are from FOCAM and the HR Cam of the Canada-France-Hawaii Telescope, and the Wide Field Camera of the Hubble Space Telescope. These are compared with previously published optical and radio images. The QSO host galaxy has the basic properties of an elliptical, but has significant irregularities, and tidal tails which indicate an ongoing gravitational interaction with a close companion.

Hutchings, J. B.↗

The line-emitting gas in active galaxies - A probe of the nuclear engine

This paper reviews some of the basic questions regarding the structure of the engine powering active galactic nuclei (AGN), the nature of the interaction between the AGN and the host galaxy, and the origin and evolution of AGN. The study of the dynamics and physical characteristics of the line-emitting gas in these objects has proven fruitful in addressing many of these issues. Recent advances in optical and infrared detector technology combined with the development of superior ground-based instruments have produced efficient new tools for the study of the line-emitting gas on nuclear and Galactic scales. Programs which take advantage of two of these new techniques, Fabry-Perot imaging spectroscopy and infrared spectroscopy, are described in this paper. The origin of nuclear activity in galaxies is also addressed in a third project which aims at determining the nature of luminous infrared galaxies.

Veilleux, Sylvain↗

Two-dimensional structure of slow shocks

The two-dimensional (2-D) structure of switch-off slow magnetosonic shocks is investigated using an electromagnetic hybrid (fluid electrons, kinetic ions) code. It is shown that the basic physical processes occurring at 1-D slow shocks are also operative in 2-D. Specifically, the interaction between the upstream ions and those streaming away from the shock results in the excitation of Alfven/ions-cyclotron (AIC) waves. Depending on the plasma parameters, these waves may either stay in the upstream or convect back into the shock resulting in a non-steady shock behavior which prevents the formation of a trailing wave train. Despite this similarity, some slow shocks which are steady in 1-D are found to be non-steady in 2-D. Fourier analysis of the waves downstream of non-steady shocks identifies them as AIC, demonstrating that the waves remain on the same branch as they convect from upstream into the downstream region.

Omidi, N.↗

A Real-Time Rover Executive based On Model-Based Reactive Planning

This paper reports on the experimental verification of the ability of IDEA (Intelligent Distributed Execution Architecture) effectively operate at multiple levels of abstraction in an autonomous control system. The basic hypothesis of IDEA is that a large control system can be structured as a collection of interacting control agents, each organized around the same fundamental structure. Two IDEA agents, a system-level agent and a mission-level agent, are designed and implemented to autonomously control the K9 rover in real-time. The system is evaluated in the scenario where the rover must acquire images from a specified set of locations. The IDEA agents are responsible for enabling the rover to achieve its goals while monitoring the execution and safety of the rover and recovering from dangerous states when necessary. Experiments carried out both in simulation and on the physical rover, produced highly promising results.

Bias, M. Bernardine↗

The Institute on Climate and Planets (ICP): A Research Education Program

Giving students a fair start to become productive and responsible contributors in the 21st century workforce and society depends on our ability to help them develop: (1) A global view of the world; (2) Problem-solving and/or reasoning abilities; (3) Basic scientific and technical literacy; and (4) A multi-disciplinary understanding of how humans and nature interact with the earth system. The Institute on Climate and Planets (ICP) in New York City is NASA Goddard Institute for Space Studies' (GISS) response to the national challenge to give students a fair start to become productive in America's workforce and society, GISS is part of the Earth Science Director at NASA Goddard Space Flight Center in Maryland and a component of Columbia University's Earth Institute, a university-wide initiative whose mission is to understand our planet so as to enhance its sustainability. In 1994 Jim Hansen, several of his GISS and Columbia University colleagues and Fitzgerald Bramwell, the former Director of the New York City Alliance for Minority Participation at City University of New York, launched the ICP. ICP contributes to NASA education and minority outreach goals by directly involving underrepresented college, high school and junior high school students and their educators in research. ICP takes advantage of the interest of many civil servants and Columbia University research scientists at GISS to involve students and educators on multi-level research teams working on problems at the core of NASA's Earth Science Enterprise - advancing our understanding of Earth s climate, climate variability, and climate impacts.

Carlson, Barbara↗

Model-based Executive Control through Reactive Planning for Autonomous Rovers

This paper reports on the design and implementation of a real-time executive for a mobile rover that uses a model-based, declarative approach. The control system is based on the Intelligent Distributed Execution Architecture (IDEA), an approach to planning and execution that provides a unified representational and computational framework for an autonomous agent. The basic hypothesis of IDEA is that a large control system can be structured as a collection of interacting agents, each with the same fundamental structure. We show that planning and real-time response are compatible if the executive minimizes the size of the planning problem. We detail the implementation of this approach on an exploration rover (Gromit an RWI ATRV Junior at NASA Ames) presenting different IDEA controllers of the same domain and comparing them with more classical approaches. We demonstrate that the approach is scalable to complex coordination of functional modules needed for autonomous navigation and exploration.

Finzi, Alberto↗

Findings from NASA's 2015-2017 Electric Sail Investigations

Electric Sail (E-Sail) propulsion systems will enable scientific spacecraft to obtain velocities of up to 10 astronomical units per year without expending any on-board propellant. The E-Sail propulsion is created from the interaction of a spacecraft's positively charged multi-kilometer-length conductor/s with protons that are present in the naturally occurring hypersonic solar wind. The protons are deflected via natural electrostatic repulsion forces from the Debye sheath that is formed around a charged wire in space, and this deflection of protons creates thrust or propulsion in the opposite direction. It is envisioned that this E-Sail propulsion system can provide propulsion throughout the solar system and to the heliosphere and beyond. Consistent with the concept of a "sail," no propellant is needed as electrostatic repulsion interactions between the naturally occurring solar wind protons and a positively charged wire creates the propulsion. The basic principle on which the Electric Sail operates is the exchange of momentum between an "electric sail" and solar wind, which continually flows radially away from the sun at speeds ranging from 300 to 700 kilometers per second. The "sail" consists of an array of long, charged wires which extend radially outward 10 to 30 kilometers from a slowly rotating spacecraft. Momentum is transferred from the solar wind to the array through the deflection of the positively charged solar wind protons by a high voltage potential applied to the wires. The thrust generated by an E-Sail is proportional to the area of the sail, which is given by the product of the total length of the wires and the effective wire diameter. The wire is approximately 0.1 millimeters in diameter. However, the effective diameter is determined by the distance the applied electric potential penetrates into space around the wire (on the order of 10 meters at 1 astronomical unit). As a result, the effective area over which protons are repelled is proportional to the size of the region of electric potential, or the plasma sheath region, surround the wires of the array. A large sheath is, therefore, beneficial to the generation of thrust. However, this benefit must be balanced with the additional fact that electron collection is proportional to sheath size. Electrons collected by the wire array must be injected back into the solar wind in order to maintain the potential on the wires - which requires power. The primary power requirement for E-Sail operation is, therefore, also proportional to sheath size.

Wiegmann, Bruce. M.↗

A New Method for Qualitative Multi-scale Analysis of Bacterial Biofilms on Filamentous Fungal Colonies Using Confocal and Electron Microscopy

Bacterial biofilms frequently form on fungal surfaces and can be involved in numerous bacterial-fungal interaction processes, such as metabolic cooperation, competition, or predation. The study of biofilms is important in many biological fields, including environmental science, food production, and medicine. However, few studies have focused on such bacterial biofilms, partially due to the difficulty of investigating them. Most of the methods for qualitative and quantitative biofilm analyses described in the literature are only suitable for biofilms forming on abiotic surfaces or on homogeneous and thin biotic surfaces, such as a monolayer of epithelial cells. While laser scanning confocal microscopy (LSCM) is often used to analyze in situ and in vivo biofilms, this technology becomes very challenging when applied to bacterial biofilms on fungal hyphae, due to the thickness and the three dimensions of the hyphal networks. To overcome this shortcoming, we developed a protocol combining microscopy with a method to limit the accumulation of hyphal layers in fungal colonies. Using this method, we were able to investigate the development of bacterial biofilms on fungal hyphae at multiple scales using both LSCM and scanning electron microscopy (SEM). Furthermore, this report describes the protocol, including microorganism cultures, bacterial biofilm formation conditions, biofilm staining, and LSCM and SEM visualizations.

59 BASIC BIOLOGICAL SCIENCES↗

Microbial Interactions Related to N 2 O Emissions and Temperature Sensitivity from Rice Paddy Fields

The soil microbiome is a driver of nitrous oxide (N 2 O) emissions in terrestrial ecosystems. Identifying the core microbiome of N 2 O emissions and its temperature sensitivity from trillions of soil microorganisms is a great challenge and is essential to improving the predictability of soil-climate feedback related to increasing temperature. Here, the integrated soil microbiome covering archaeal, bacterial, fungal, algal, and microfaunal communities was studied to disengage the potential linkage with its N 2 O emissions and its temperature sensitivity in paddy fields by hunting for core species pairs. The results showed that between-group interactions of core bacterial and archaeal members and the within-group interactions of core bacterial members jointly contributed to the N 2 O emissions and its temperature sensitivity. The contribution of between-group interactions (32 to 33%) was greater than that of within groups (10 to 18%). These results suggested that N 2 O emissions and their fluctuations related to climate warming are affected by the within- and between-group interactions of the soil microbiome. Our results help advance the knowledge on the importance of microbial keystone species and network associations in controlling N 2 O production and their responses to increasing temperature.

59 BASIC BIOLOGICAL SCIENCES↗

Structural and Biochemical Characterization of Human Adenylosuccinate Lyase (ADSL) and the R303C ADSL Deficiency-Associated Mutation

Adenylosuccinate lyase (ADSL) deficiency is a rare autosomal recessive disorder, which causes a defect in purine metabolism resulting in neurological and physiological symptoms. ADSL executes two nonsequential steps in the de novo synthesis of AMP: the conversion of phosphoribosylsuccinyl-aminoimidazole carboxamide (SAICAR) to phosphoribosylaminoimidazole carboxamide, which occurs in the de novo synthesis of IMP, and the conversion of adenylosuccinate to AMP, which occurs in the de novo synthesis of AMP and also in the purine nucleotide cycle, using the same active site. Mutation of ADSL's arginine 303 to a cysteine is known to lead to ADSL deficiency. Interestingly, unlike other mutations leading to ADSL deficiency, the R303C mutation has been suggested to more significantly affect the enzyme's ability to catalyze the conversion of succinyladenosine monophosphate than that of SAICAR to their respective products. To better understand the causation of disease due to the R303C mutation, as well as to gain insights into why the R303C mutation potentially has a disproportional decrease in activity toward its substrates, the wild type (WT) and the R303C mutant of ADSL were investigated enzymatically and thermodynamically. Additionally, the X-ray structures of ADSL in its apo form as well as with the R303C mutation were elucidated, providing insight into ADSL's cooperativity. By utilizing this information, a model for the interaction between ADSL and SAICAR is proposed.

59 BASIC BIOLOGICAL SCIENCES↗

Comprehensive analysis of the human ESCRT-III-MIT domain interactome reveals new cofactors for cytokinetic abscission

The 12 related human ESCRT-III proteins form filaments that constrict membranes and mediate fission, including during cytokinetic abscission. The C-terminal tails of polymerized ESCRT-III subunits also bind proteins that contain Microtubule-Interacting and Trafficking (MIT) domains. MIT domains can interact with ESCRT-III tails in many different ways to create a complex binding code that is used to recruit essential cofactors to sites of ESCRT activity. Here, we have comprehensively and quantitatively mapped the interactions between all known ESCRT-III tails and 19 recombinant human MIT domains. We measured 228 pairwise interactions, quantified 60 positive interactions, and discovered 18 previously unreported interactions. We also report the crystal structure of the SPASTIN MIT domain in complex with the IST1 C-terminal tail. Three MIT enzymes were studied in detail and shown to: (1) localize to cytokinetic midbody membrane bridges through interactions with their specific ESCRT-III binding partners (SPASTIN-IST1, KATNA1-CHMP3, and CAPN7-IST1), (2) function in abscission (SPASTIN, KATNA1, and CAPN7), and (3) function in the ‘NoCut’ abscission checkpoint (SPASTIN and CAPN7). Our studies define the human MIT-ESCRT-III interactome, identify new factors and activities required for cytokinetic abscission and its regulation, and provide a platform for analyzing ESCRT-III and MIT cofactor interactions in all ESCRT-mediated processes.

59 BASIC BIOLOGICAL SCIENCES↗

Novel Insights Into The Mode of Inhibition of Class A SHV-1 Beta-Lactamases Revealed by Boronic Acid Transition State Inhibitors

Boronic acid transition state inhibitors (BATSIs) are potent class A and C {beta}-lactamase inactivators and are of particular interest due to their reversible nature mimicking the transition state. Here, we present structural and kinetic data describing the inhibition of the SHV-1 {beta}-lactamase, a clinically important enzyme found in Klebsiella pneumoniae, by BATSI compounds possessing the R1 side chains of ceftazidime and cefoperazone and designed variants of the latter, compounds 1 and 2. The ceftazidime and cefoperazone BATSI compounds inhibit the SHV-1 {beta}-lactamase with micromolar affinity that is considerably weaker than their inhibition of other {beta}-lactamases. The solved crystal structures of these two BATSIs in complex with SHV-1 reveal a possible reason for SHV-1's relative resistance to inhibition, as the BATSIs adopt a deacylation transition state conformation compared to the usual acylation transition state conformation when complexed to other {beta}-lactamases. Active-site comparison suggests that these conformational differences might be attributed to a subtle shift of residue A237 in SHV-1. The ceftazidime BATSI structure revealed that the carboxyl-dimethyl moiety is positioned in SHV-1's carboxyl binding pocket. In contrast, the cefoperazone BATSI has its R1 group pointing away from the active site such that its phenol moiety moves residue Y105 from the active site via end-on stacking interactions. To work toward improving the affinity of the cefoperazone BATSI, we synthesized two variants in which either one or two extra carbons were added to the phenol linker. Both variants yielded improved affinity against SHV-1, possibly as a consequence of releasing the strain of its interaction with the unusual Y105 conformation.

59 BASIC BIOLOGICAL SCIENCES↗

Monte Carlo study of double exchange interaction in manganese oxide

In this paper we study the magnetoresistance properties attributed by double exchange (DE) interaction in manganese oxide by Monte Carlo simulation. We construct a model based on mixed-valence Mn{sup 3+} and Mn{sup 4+} on the general system of Re{sub 2/3}Ae{sub 1/3}MnO{sub 3} in two dimensional system. The conduction mechanism is based on probability of e{sub g} electrons hopping from Mn{sup 3+} to Mn{sup 4+}. The resistivity dependence on temperature and the external magnetic field are presented and the validity with related experimental results are discussed. We use the resistivity power law to fit our data on metallic region and basic activated behavior on insulator region. On metallic region, we found our result agree well with the quantum theory of DE interaction. From general arguments, we found our simulation agree qualitatively with experimental results.

75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND↗