Genetic Screen for Cell Fitness in High or Low Oxygen Highlights Mitochondrial and Lipid Metabolism
Not Available
59 BASIC BIOLOGICAL SCIENCES↗
SEARCH · Engineering Papers
Results for “metabolic”
Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.
Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.
Functional genetics of human gut commensal Bacteroides thetaiotaomicron reveals metabolic requirements for growth across environments
Not Available
59 BASIC BIOLOGICAL SCIENCES↗
Discovery, characterization, and metabolic engineering of Rieske non-heme iron monooxygenases for guaiacol O-demethylation
Aryl-O-demethylation is a common rate-limiting step in the catabolism of lignin-related compounds, including guaiacol. In this work, we used randomly barcoded transposon insertion sequencing (RB-TnSeq) in the bacterium Novosphingobium aromaticivorans to identify a Rieske-type guaiacol O-demethylase, GdmA. Similarity searches identified GdmA homologs in other bacteria, along with candidate reductase partners, denoted GdmB. GdmAB combinations were biochemically characterized for activity with several lignin-related substrates. Structural and sequence comparisons of vanillate- and guaiacol-specific O-demethylase active sites revealed conserved hallmarks of substrate specificity. GdmAB combinations were also evaluated in Pseudomonas putida KT2440, which does not natively utilize guaiacol. GdmAB from Cupriavidus necator N-1 demonstrated the highest rate of guaiacol turnover in vitro and in engineered P. putida strains and notably higher catalytic efficiency than a cytochrome P450 system (GcoAB) and the vanillate Rieske-type O-demethylase from P. putida (VanAB). The GdmAB O-demethylases described here expand the suite of options for microbial conversion of a model lignin-derived substrate.
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
Metabolic Modifier Screen Reveals Secondary Targets of Protein Kinase Inhibitors within Nucleotide M
Explore the source record for details and available documents.
Abt, Evan R.↗
Tracing metabolic flux through time and space with isotope labeling experiments
Not Available
Allen, Doug K.↗
Transcriptional, metabolic, physiological and developmental responses to nitrogen limitation in switchgrass (Panicum virgatum)
Not Available
Environmental Sciences & Ecology↗
Integrative LC-MS and GC-MS metabolic profiling unveils dynamic changes during barley malting
Not Available
Rani, Heena↗
Fungal endophytes of the invasive grass Eragrostis lehmanniana shift metabolic expression in response to native and invasive grasses
Not provided.
Environmental Sciences & Ecology↗
Expression Analysis of Genes Related to Auxin Metabolism at Different Growth Stages of Pak Choi
Not Available
Hou, Yuxiang↗
Impact of Pelvic Radiation Therapy for Prostate Cancer on Global Metabolic Profiles and Microbiota-Driven Gastrointestinal Late Side Effects: A Longitudinal Observational Study
Radiation therapy to the prostate and pelvic lymph nodes (PLNRT) is part of the curative treatment of high-risk prostate cancer. Yet, the broader influence of radiation therapy on patient physiology is poorly understood. We conducted comprehensive global metabolomic profiling of urine, plasma, and stools sampled from patients undergoing PLNRT for high-risk prostate cancer.
62 RADIOLOGY AND NUCLEAR MEDICINE↗