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Sister Rod Destructive Examinations (FY23) Appendix I: SNF Aerosols Released During Rod Fracture

This report documents work performed under the Spent Fuel and Waste Disposition’s Spent Fuel and Waste Science and Technology program for the US Department of Energy (DOE) Office of Nuclear Energy (NE). This work was performed to fulfill Level 3 Milestone M3SF-23OR010201026, “FY23 M3 draft report on results from testing in FY23,” within work package SF-23OR01020102 and is an updated to the work reported in M2SF-23OR010201024, M2SF-22OR010201047, M2SF-21OR010201032, M2SF-19ORO010201026, and M2SF-19OR010201028. As a part of the DOE NE High Burnup Spent Fuel Data Project, Oak Ridge National Laboratory (ORNL) is performing destructive examinations (DEs) of high burnup (HBU) (>45 GWd/MTU) spent nuclear fuel (SNF) rods from the North Anna Nuclear Power Station operated by Dominion Energy. The SNF rods, called sister rods or sibling rods, are all HBU and include four different kinds of fuel rod cladding: standard Zircaloy-4 (Zirc-4), low-tin Zirc-4, ZIRLO, and M5. The DEs are being conducted to obtain a baseline of the HBU rods’ condition before dry storage and are focused on understanding overall SNF rod strength and durability. Fuel rods and defueled cladding will be tested to derive material properties. Although the data generated can be used for multiple purposes, one primary goal for obtaining the post-irradiation examination data and the associated measured mechanical properties is to support SNF dry storage licensing and relicensing activities by (1) addressing identified knowledge gaps and (2) enhancing the technical basis for post-storage transportation, handling, and subsequent disposition.This report documents the status of the ORNL Phase 1 DE activities related to the collection of SNF aerosol particles released during fuel rod fracture in 4-point bending in Phase 1 of the sister rod test program.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗

A Concept of Operations for Far-Term Surface Trajectory-Based Operations (STBO)

The goal of this far-term STBO (Surface Trajectory-Based Operations) ConOps (Concept of Operations) is to increase the efficiency and predictability of airport surface operations, and reduce the environmental impact, by incorporating a time-based component to surface operations. In the far-term NextGen timeframe, airport surface operations will transition from current-day first-come, first-served operations, to strategically scheduled operations in which pilots are recruited as active participants in meeting the precise time-based goals of NextGen surface operations. The far-term STBO concept includes two-phases. Phase 1 introduces time-based traffic flow constraint points, which divide the taxi route into segments with an assigned Required Time of Arrival (RTA). This Phase 1 approach provides temporal certainty only near the traffic flow constraint points, but not in between. Minimal augmentations to the flight deck are required to support required time of arrival (RTA) management. Phase 2 further increases precision and efficiency by introducing full four-dimensional (4D) trajectories, with an x-y location for all times t. This phase assumes adoption of advanced flight deck equipage enabling higher temporal precision sufficient to support aircraft conformance to 4D trajectories. This allows more precision and less temporal uncertainty at all times along the route.

Surface Trajectory-Based Operations (STBO)↗

Sister Rod Destructive Examinations (FY2021) Appendix F: Cyclic Integrated Reversible-Bending Fatigue Tests

As a part of DOE NE High Burnup Spent Fuel Data Project, Oak Ridge National Laboratory (ORNL) is performing destructive examinations (DEs) of high burnup (HBU) (>45 GWd/MTU) spent nuclear fuel (SNF) rods from the North Anna Nuclear Power Station operated by Dominion Energy. The SNF rods, called sister rods or sibling rods, are all HBU and include four different kinds of fuel rod cladding: standard Zircaloy-4 (Zirc-4), low-tin (LT) Zirc-4, ZIRLO, and M5. This appendix documents the status of the ORNL Phase 1 DE activities related to tests of the sister rods using the Cyclic Integrated Reversible-Bending Fatigue Tester (CIRFT) in Phase 1 of the sister rod test program.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗

Sister Rod Destructive Examinations (FY22) Appendix I: SNF Aerosols Released During Rod Fracture

As a part of the DOE NE High Burnup Spent Fuel Data Project, Oak Ridge National Laboratory (ORNL) is performing destructive examinations (DEs) of high burnup (HBU) (>45 GWd/MTU) spent nuclear fuel (SNF) rods from the North Anna Nuclear Power Station operated by Dominion Energy. The SNF rods, called sister rods or sibling rods, are all HBU and include four different kinds of fuel rod cladding: standard Zircaloy-4 (Zirc-4), low-tin Zirc-4, ZIRLO, and M5. The DEs are being conducted to obtain a baseline of the HBU rods’ condition before dry storage and are focused on understanding overall SNF rod strength and durability. Fuel rods and defueled cladding will be tested to derive material properties. Although the data generated can be used for multiple purposes, one primary goal for obtaining the post-irradiation examination data and the associated measured mechanical properties is to support SNF dry storage licensing and relicensing activities by (1) addressing identified knowledge gaps and (2) enhancing the technical basis for post-storage transportation, handling, and subsequent disposition. This report documents the status of the ORNL Phase 1 DE activities related to the collection of SNF aerosol particles released during fuel rod fracture in 4-point bending in Phase 1 of the sister rod test program.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗

Sister Rod Destructive Examinations (FY22) Appendix F: Cyclic Integrated Reversible-Bending Fatigue Tests

This report documents work performed under the Spent Fuel and Waste Disposition’s Spent Fuel and Waste Science and Technology program for the US Department of Energy (DOE) Office of Nuclear Energy (NE). This work was performed to fulfill Level 3 Milestone M3SF-22OR010201046, “Draft FY2022 ORNL Report on High Burnup Sibling Pin Testing Results,” within work package SF-22OR01020104 and is an update to the work reported in M2SF-22OR010201047, M2SF-21OR010201032, M2SF-19ORO010201026 and M2SF-19OR010201028. As a part of DOE NE High Burnup Spent Fuel Data Project, Oak Ridge National Laboratory (ORNL) is performing destructive examinations (DEs) of high burnup (HBU) (>45 GWd/MTU) spent nuclear fuel (SNF) rods from the North Anna Nuclear Power Station operated by Dominion Energy. The SNF rods, called sister rods or sibling rods, are all HBU and include four different kinds of fuel rod cladding: standard Zircaloy-4 (Zirc-4), low-tin (LT) Zirc-4, ZIRLO, and M5. The DEs are being conducted to obtain a baseline of the HBU rods’ condition before dry storage and are focused on understanding overall SNF rod strength and durability. Composite fuel and defueled cladding will be tested to derive material properties. Although the data generated can be used for multiple purposes, one primary goal for obtaining the post-irradiation examination data and the associated measured mechanical properties is to support SNF dry storage licensing and relicensing activities by (1) addressing identified knowledge gaps and (2) enhancing the technical basis for post-storage transportation, handling, and subsequent disposition. This appendix documents the status of the ORNL Phase 1 DE activities related to tests of the sister rods using the Cyclic Integrated Reversible-Bending Fatigue Tester (CIRFT) in Phase 1 of the sister rod test program.

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Sister Rod Destructive Examinations (FY23) Appendix D: Fission Gas, Fuel Burnup, and Fuel Isotopic Measurements

As a part of DOE NE High Burnup Spent Fuel Data Project, Oak Ridge National Laboratory (ORNL) is performing destructive examinations (DEs) of high burnup (HBU) (>45 GWd/MTU) spent nuclear fuel (SNF) rods from the North Anna Nuclear Power Station operated by Dominion Energy. The SNF rods, called sister rods or sibling rods, are all HBU and include four different kinds of fuel rod cladding: standard Zircaloy-4 (Zirc-4), low-tin (LT) Zirc-4, ZIRLO, and M5. The DEs are being conducted to obtain a baseline of the HBU rod’s condition before dry storage and are focused on understanding overall SNF rod strength and durability. Composite fuel and defueled cladding will be tested to derive material properties. Although the data generated can be used for multiple purposes, one primary goal for obtaining the post-irradiation examination data and the associated measured mechanical properties is to support SNF dry storage licensing and relicensing activities by (1) addressing identified knowledge gaps and (2) enhancing the technical basis for post-storage transportation, handling, and subsequent disposition. This appendix documents the status of the ORNL Phase 1 DE activities related to fission gas sample analysis, fuel burnup analysis, and fuel isotopic analysis of selected sister specimens in Phase 1 of the sister rod test program.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗

Airspace Technology Demonstration 2 (ATD-2): Analysis of APREQ Flights at CLT

5/22/2019 National Aeronautics and Space Administration 1 Airspace Technology Demonstration 2 (ATD-2) Analysis of APREQ Flights at CLT May 22, 2019 5/22/2019 Objective 2 Quantify impact of IADS Phase 1 & 2 capabilities on APREQ flights at CLT with respect to: • Compliance to the Controlled Take Off Time (CTOT) • Benefits for APREQ flights that use IDAC to renegotiate for an earlier CTOT • Benefits of pre-scheduling APREQ flights using the Earliest Off Block Time (EOBT) • Relationship between EOBT compliance and rescheduling CTOT 5/22/2019 CLT APREQ Daily Compliance(Compliance Improvement Since ATD-2 Start) 3 Steady increase of APREQ compliance over the life of the project. Reduced variation in compliance leading to improved predictability. In addition to overall improved compliance into TBM systems, the predictability is increasing 5/22/2019 APREQ Compliance 10K Rolling Window 4 The most substantial APREQ compliance improvements started with Phase 2 capability (AEFS integration, ZTL IDAC, pre-scheduling and scheduler updates). 5/22/2019 IADS Phase 1 & 2 Benefit Mechanisms 5 1. Collaborative surface metering •Reduced engine run time •Reduced fuel consumption and emissions 2. Overhead stream operational integration a.Scheduling controlled flights at the gate •Reduced engine run time •Reduced fuel consumption and emissions b.APREQ renegotiating for an earlier slot •Reduced total delay •Passenger value of time and crew costs •Reduced engine run time •Reduced fuel consumption and emissions Benefits (1) and (2a) achieved through tactical gate holds Benefit (2b) achieved through APREQ renegotiation process described below Step 1: APREQ flight has a release time but is capable of taking off earlier Step 2: FAA TMC uses the IDAC green space / red space to identify and request an earlierslot in the overhead stream Step 3: Aircraft receives earlierrelease time and the difference between the release times is the reduction in delay 5/22/2019 Benefits for APREQ flights using IDAC to renegotiate for earlierCTOT 6 LBS Fuel 270.7 hours of delay saved by electronically renegotiating a better overhead stream time for 2,071 flights. • The benefits described here are associated with better use of existing capacity in the overhead stream, and technology to reduce surface delay. •These benefits are in addition to (distinct from) surface metering savings. 5/22/2019 APREQ Delay For Pre-Scheduled Flights into KATL Have Been Reduced and are More Predictable For the Last Five Months 7 Substantial Improvements in predictability of delay for the last 5 months 5/22/2019 EOBT Compliance / CTOT Reschedulefor Pre-Scheduled Flights into KATL 8 5/22/2019 Wrap-up 9 • Compliance to the CTOT has improved throughout the lifecycle of ATD-2 with biggest improvements following the introduction of Phase 2 capabilities • Rescheduling APREQ flights using IDAC has reduced 270.7 hours of delay at CLT • Predictability of local surface delay for APREQ flights is substantially improved via pre-scheduling with the IADS system • Pre-scheduled flights that reschedule for later times tend to call ready later with respect to EOBT

CLT↗

Alteration of cell cycle progression by Sindbis virus infection

We examined the impact of Sindbis virus (SINV) infection on cell cycle progression in a cancer cell line, HeLa, and a non-cancerous cell line, Vero. Cell cycle analyses showed that SINV infection is able to alter the cell cycle progression in both HeLa and Vero cells, but differently, especially during the early stage of infection. SINV infection affected the expression of several cell cycle regulators (CDK4, CDK6, cyclin E, p21, cyclin A and cyclin B) in HeLa cells and caused HeLa cells to accumulate in S phase during the early stage of infection. Monitoring SINV replication in HeLa and Vero cells expressing cell cycle indicators revealed that SINV which infected HeLa cells during G{sub 1} phase preferred to proliferate during S/G{sub 2} phase, and the average time interval for viral replication was significantly shorter in both HeLa and Vero cells infected during G{sub 1} phase than in cells infected during S/G{sub 2} phase. - Highlights: • SINV infection was able to alter the cell cycle progression of infected cancer cells. • SINV infection can affect the expression of cell cycle regulators. • SINV infection exhibited a preference for the timing of viral replication among the cell cycle phases.

60 APPLIED LIFE SCIENCES↗

Downregulation of miR-210 expression inhibits proliferation, induces apoptosis and enhances radiosensitivity in hypoxic human hepatoma cells in vitro

Hypoxia is a common feature of solid tumors and an important contributor to tumor radioresistance. miR-210 is the most consistently and robustly induced microRNA under hypoxia in different types of tumor cells and normal cells. In the present study, to explore the feasibility of miR-210 as an effective therapeutic target, lentiviral-mediated anti-sense miR-210 gene transfer technique was employed to downregulate miR-210 expression in hypoxic human hepatoma SMMC-7721, HepG2 and HuH7 cells, and phenotypic changes of which were analyzed. Hypoxia led to an increased hypoxia inducible factor-1{alpha} (HIF-1{alpha}) and miR-210 expression and cell arrest in the G{sub 0}/G{sub 1} phase in all cell lines. miR-210 downregulation significantly suppressed cell viability, induced cell arrest in the G{sub 0}/G{sub 1} phase, increased apoptotic rate and enhanced radiosensitivity in hypoxic human hepatoma cells. Moreover, apoptosis-inducing factor, mitochondrion-associated, 3 (AIFM3) was identified as a direct target gene of miR-210. AIFM3 downregulation by siRNA attenuated radiation induced apoptosis in miR-210 downregulated hypoxic human hepatoma cells. Taken together, these data suggest that miR-210 might be a potential therapeutic target and specific inhibition of miR-210 expression in combination with radiotherapy might be expected to exert strong anti-tumor effect on hypoxic human hepatoma cells. -- Highlights: Black-Right-Pointing-Pointer miR-210 downregulation radiosensitized hypoxic hepatoma. Black-Right-Pointing-Pointer AIFM3 was identified as a direct target gene of miR-210. Black-Right-Pointing-Pointer miR-210 might be a therapeutic target to hypoxic hepatoma.

62 RADIOLOGY AND NUCLEAR MEDICINE↗

Sister Rod Destructive Examinations (FY2020) Appendix I: SNF Aerosols Released During Rod Fracture

As a part of the DOE NE High Burnup Spent Fuel Data Project, Oak Ridge National Laboratory (ORNL) is performing destructive examinations (DEs) of high burnup (HBU) (>45 GWd/MTU) spent nuclear fuel (SNF) rods from the North Anna Nuclear Power Station operated by Dominion Energy. The SNF rods, called sister rods or sibling rods, are all HBU and include four different kinds of fuel rod cladding: standard Zircaloy-4 (Zirc-4), low-tin Zirc-4, ZIRLO, and M5. This report documents the status of the ORNL Phase 1 DE activities related to the collection of SNF aerosol particles released during fuel rod fracture in four-point bending in Phase 1 of the sister rod test program.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗

Boeing Crew Exploration Vehicle Environmental Control and Life Support System Architecture Overview

The Boeing Company under the teaming agreement with the Northrop Grumman Systems Corporation and in compliance with the NASA Phase 1 contract, had the responsibilities for the CEV architecture development of the Environmental control and life support (ECLS) system under the NASA Phase 1 contract. The ECLS system was comprised of the various subsystems which provided for a shirt-sleeve habitable environment for crew to live and work in the crew module of the CEV. This architecture met the NASA requirements to ferry cargo and crew to ISS, and Lunar sortie missions, with extensibility to long duration missions to Moon and MARS. This paper provides a summary overview of the CEV ECLS subsystems which was proposed in compliance with the contract activities.

Saiidi, Mo↗

Sister Rod Destructive Examinations (FY23) Appendix C: Rod Internal Pressure, Void Volume, and Gas Transmission Tests

As a part of the DOE NE High Burnup Spent Fuel Data Project, Oak Ridge National Laboratory (ORNL) is performing destructive examinations (DEs) of high burnup (HBU) (>45 GWD/MTU) spent nuclear fuel (SNF) rods from the North Anna Nuclear Power Station operated by Dominion Energy. The SNF rods, called the sister rods or sibling rods, are all HBU and include four different kinds of fuel rod cladding: standard Zircaloy-4 (Zirc-4), low-tin (LT) Zirc-4, ZIRLO, and M5. The DEs are being conducted to obtain a baseline of the HBU rod’s condition before dry storage and are focused on understanding overall SNF rod strength and durability. Both fuel rods and empty cladding will be tested to derive material properties. Although the data generated can be used for multiple purposes, one primary goal for obtaining the postirradiation examination data and the associated measured mechanical properties is to support SNF dry storage licensing and relicensing activities by (1) addressing identified knowledge gaps and (2) enhancing the technical basis for post-storage transportation, handling, and consolidation activities. This appendix documents the status of the ORNL Phase 1 DE activities related to rod internal pressure and void volume measurement techniques, fission gas stack flow measurements applied to selected sister rods, and fission gas release calculations in Phase 1 of the sister rod test program.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗

Large-scale static investigation of circulation-control-wing concepts applied to upper surface-blowing aircraft

The use of a circulation control to deflect turbofan engine thrust beyond 90 deg. has been proven in full-scale static ground tests of the circulation-control-wing/upper-surface-blowing (CCW/USB) concept. This powered high-lift system employs a circular, blown trailing edge to replace the USB mechanical flaps to entrain engine-exhaust flow, and to obtain both a vertical-thrust component and an augmented circulation lift for short takeoff and landing (STOL) applications. Previous tests (Phase 1), done in 1982, of a basic configuration installed on the Quiet Short Haul Research Aircraft confirmed these CCW/USB systems capabilities. A second phase (Phase 2) of full-scale, static, thrust-deflection investigations has reconfirmed the ability to deflect engine thrust from 40 to 102 deg., depending on thrust level. Five new configurations were evaluated and performance improvements noted for those configurations with larger blown span, fences or favorable engine interactions, smaller slot height, and larger radii with less than 180 deg. of CCW surface arc. In general, a 90 deg. circular arc with a smaller slot height provided the best performance, demonstrating that adequate thrust turning can be produced by a trailing-edge shape which may have minimal cruise-performance penalty. Thrust deflections were achieved at considerably lower blowing momentum than was required for the baseline case of Phase 1. Improved performance and versatility were thus confirmed for the CCW/USB system applied to STOL aircraft, where the potential for developing a non-moving-parts pneumatic thrust deflector to rapidly vary horizontal force from thrust to drag, while maintaining constant vertical force, appears quite promising. The conversion from high-lift to lower-drag cruise mode by merely terminating the blowing provides an effective STOL aircraft system.

Shovlin, M. D.↗

NASA UAS Integration into the NAS Project Detect and Avoid Display Evaluations

As part of the Air Force - NASA Bi-Annual Research Council Meeting, slides will be presented on phase 1 Detect and Avoid (DAA) display evaluations. A series of iterative human-in-the-loops (HITL) experiments were conducted with different display configurations to objectively measure pilot performance on maintaining well clear. To date, four simulations and two mini-HITLs have been conducted. Data from these experiments have been incorporated into a revised alerting structure and included in the RTCA SC 228 Phase 1 Minimum Operational Performance Standards (MOPS) proposal. Plans for phase 2 are briefly discussed.

Unmanned Aircraft Systems (UAS)↗

Sister Rod Destructive Examinations (FY22) Appendix C: Rod Internal Pressure, Void Volume, and Gas Transmission Tests

As a part of the DOE NE High Burnup Spent Fuel Data Project, Oak Ridge National Laboratory (ORNL) is performing destructive examinations (DEs) of high burnup (HBU) (>45 GWD/MTU) spent nuclear fuel (SNF) rods from the North Anna Nuclear Power Station operated by Dominion Energy [C-1]. The SNF rods, called the sister rods or sibling rods, are all HBU and include four different kinds of fuel rod cladding: standard Zircaloy-4 (Zirc-4), low-tin (LT) Zirc-4, ZIRLO, and M5. The DEs are being conducted to obtain a baseline of the HBU rod’s condition before dry storage and are focused on understanding overall SNF rod strength and durability. Both fuel rods and empty cladding will be tested to derive material properties. Although the data generated can be used for multiple purposes, one primary goal for obtaining the postirradiation examination data and the associated measured mechanical properties is to support SNF dry storage licensing and relicensing activities by (1) addressing identified knowledge gaps and (2) enhancing the technical basis for post-storage transportation, handling, and consolidation activities. This appendix documents the status of the ORNL Phase 1 DE activities [C-2, C-3] related to rod internal pressure and void volume measurement techniques, fission gas stack flow measurements applied to selected sister rods, and fission gas release calculations in Phase 1 of the sister rod test program.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗

Affordable Flight Demonstration of the GTX Air-Breathing SSTO Vehicle Concept

The rocket based combined cycle (RBCC) powered single-stage-to-orbit (SSTO) reusable launch vehicle has the potential to significantly reduce the total cost per pound for orbital payload missions. To validate overall system performance, a flight demonstration must be performed. This paper presents an overview of the first phase of a flight demonstration program for the GTX SSTO vehicle concept. Phase 1 will validate the propulsion performance of the vehicle configuration over the supersonic and hypersonic airbreathing portions of the trajectory. The focus and goal of Phase 1 is to demonstrate the integration and performance of the propulsion system flowpath with the vehicle aerodynamics over the air-breathing trajectory. This demonstrator vehicle will have dual mode ramjet/scramjets, which include the inlet, combustor, and nozzle with geometrically scaled aerodynamic surface outer mold lines (OML) defining the forebody, boundary layer diverter, wings, and tail. The primary objective of this study is to demonstrate propulsion system performance and operability including the ram to scram transition, as well as to validate vehicle aerodynamics and propulsion airframe integration. To minimize overall risk and development cost the effort will incorporate proven materials, use existing turbomachinery in the propellant delivery systems, launch from an existing unmanned remote launch facility, and use basic vehicle recovery techniques to minimize control and landing requirements. A second phase would demonstrate propulsion performance across all critical portions of a space launch trajectory (lift off through transition to all-rocket) integrated with flight-like vehicle systems.

Krivanek, Thomas M.↗