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At least 1,135 records · Page 63
All sourcesNASA NTRSDOE OSTI
DOE OSTI2014

Effect of the wire width on the intrinsic detection efficiency of superconducting-nanowire single-photon detectors

A thorough spectral study of the intrinsic single-photon detection efficiency in superconducting TaN and NbN nanowires with different widths has been performed. The experiment shows that the cut-off of the intrinsic detection efficiency at near-infrared wavelengths is most likely controlled by the local suppression of the barrier for vortex nucleation around the absorption site. Beyond the cut-off quasi-particle diffusion in combination with spontaneous, thermally activated vortex crossing explains the detection process. For both materials, the reciprocal cut-off wavelength scales linearly with the wire width where the scaling factor agrees with the hot-spot detection model.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗
DOE OSTI2024

Biphasic response of human iPSC-derived neural network activity following exposure to a sarin-surrogate nerve agent

Organophosphorus nerve agents (OPNA) are hazardous environmental exposures to the civilian population and have been historically weaponized as chemical warfare agents (CWA). OPNA exposure can lead to several neurological, sensory, and motor symptoms that can manifest into chronic neurological illnesses later in life. There is still a large need for technological advancement to better understand changes in brain function following OPNA exposure. The human-relevant in vitro multi-electrode array (MEA) system, which combines the MEA technology with human stem cell technology, has the potential to monitor the acute, sub-chronic, and chronic consequences of OPNA exposure on brain activity. However, the application of this system to assess OPNA hazards and risks to human brain function remains to be investigated. In a concentration-response study, we have employed a human-relevant MEA system to monitor and detect changes in the electrical activity of engineered neural networks to increasing concentrations of the sarin surrogate 4-nitrophenyl isopropyl methylphosphonate (NIMP). We report a biphasic response in the spiking (but not bursting) activity of neurons exposed to low (i.e., 0.4 and 4 μM) versus high concentrations (i.e., 40 and 100 μM) of NIMP, which was monitored during the exposure period and up to 6 days post-exposure. Regardless of the NIMP concentration, at a network level, communication or coordination of neuronal activity decreased as early as 60 min and persisted at 24 h of NIMP exposure. Once NIMP was removed, coordinated activity was no different than control (0 μM of NIMP). Interestingly, only in the high concentration of NIMP did coordination of activity at a network level begin to decrease again at 2 days post-exposure and persisted on day 6 post-exposure. Notably, cell viability was not affected during or after NIMP exposure. Also, while the catalytic activity of AChE decreased during NIMP exposure, its activity recovered once NIMP was removed. Gene expression analysis suggests that human iPSC-derived neurons and primary human astrocytes resulted in altered genes related to the cell’s interaction with the extracellular environment, its intracellular calcium signaling pathways, and inflammation, which could have contributed to how neurons communicated at a network level.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2012

The Importance of Establishing and Maintaining Continuity of Knowledge during 21st Century Nuclear Fuel Cycle Activities

During this century, the entire nuclear fuel cycle will expand and become increasingly more global, taxing both the resources and capabilities of the International Atomic Energy Agency (IAEA) to maintain an effective Continuity of Knowledge (CoK) and its ability to provide timely detection of diversion. Uranium that currently is mined and milled in one country will be converted, enriched, and fabricated into fuel for reactors in an expanding set of new countries. This expansion will make it harder to guarantee that regional activities stay regional and that diversion detection is timely unless new and sustainable tools are developed to improve inspector effectiveness. To deal with this emerging reality, the IAEA must increase its use of unattended monitoring and employ new tools and methods that enhance CoK during all phases of the fuel cycle. This approach will help provide useful information to aid in detecting undeclared activities and create opportunities for timely and appropriate responses to events well before they enter phases of greater concern (e.g., enrichment). The systems that maintain CoK of safeguarded assets rely on containment and surveillance (C/S) technologies. The 21st century fuel cycle will require increased use of these technologies and systems, plus greater implementation of unattended systems that can securely collect data when inspectors are not present.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗
DOE OSTI2016

Proteomic Stable Isotope Probing Reveals Biosynthesis Dynamics of Slow Growing Methane Based Microbial Communities

Marine methane seep habitats represent an important control on the global flux of methane between the subsurface and water column reservoirs. Meta-omics studies have begun to outline community-wide metabolic potential, but expression patterns of proteins that enact sulfate-mediated anaerobic methane oxidation in seeps are poorly characterized. Proteomic stable isotope probing (proteomic SIP) offers an additional layer of information for characterizing phylogenetically specific, functionally relevant activity in mixed microbial communities. Here we applied proteomic SIP to 15NH4+ and CH4 amended seep sediment microcosms in an attempt to track the protein synthesis of slow-growing, low-energy microbial systems. Across all samples, 3495 proteins were identified, 21% of which were 15N-labeled. We observed active synthesis (15N enrichment) of all proteins believed to be involved in sulfate reduction and reverse methanogenesis including methylenetetrahydromethanopterin reductase (Mer). The abundance and phylogenetic range of methyl-coenzyme M reductase (Mcr) orthologs produced during incubation experiments suggests that seeps provide sufficient niches for multiple organisms performing analogous metabolisms. Twenty-eight previously unreported post-translational modifications of McrA were measured, indicating dynamic enzymatic machinery and offering a dimension of functional diversity beyond gene-dictated sequence. RNA polymerase associated with putative sulfur-oxidizing Epsilonproteobacteria and aerobic Gammaproteobacteria were more abundant among pre-incubation proteins, suggesting diminished metabolic activity in long-term anoxic, sulfidic experimental incubations. Twenty-six proteins of unknown function were detected in all proteomic experiments and actively expressed in labeled experiments, suggesting that they play important roles in methane seep ecosystems. The addition of stable isotope probing to environmental proteomics experiments provides a mechanism to begin to assess the degree to which diagnostic meatbolic proteins are long-lived or acively synthesized in complex, slow-growing microbial communities. Our work here demonstrates that sediment-hosted microbial assemblages in marine methane seeps are dynamic, heterogeneous systems with broad functional diversity.

59 BASIC BIOLOGICAL SCIENCES↗
NASA NTRS1982

The intercrater plains of Mercury and the Moon: Their nature, origin and role in terrestrial planet evolution. Chronology of surface history of Mercury

Phases in the history of the planet Mercury include: (1) condensation and accretion; (2) heating; (3) planetary expansion during heavy bombardment; (4) tidal spin-down and lineament formation; (5) P5 plains emplacement; (6) P4 plains emplacement; (7) peak planetary volume in P3 period; (8) scarp formation; (9) Caloris Basin formation, late class 3; (10) scarp formation and P2 plains formation; (11) smooth plains formation in and around large basins; (12) late or local tectonic stress; and (13) quiescent class 1 period. Although the cooling and contraction of the lithosphere are complete, the core remains molten as an active dynamo, producing the magnetic fields detected by Mariner 10. Plains produced since core formation (P3 to P-1) should record its magnetic activity. Cratering during the Class 2 and Class 1 periods is probably not enough to distribute ballistic materials and homogenize any color differences.

Leake, M. A.↗
NASA NTRS1996

Validation of Spectral Analysis as a Noninvasive Tool to Assess Autonomic Regulation of Cardiovascular Function

A major focus of our program has been to develop a sensitive noninvasive procedure to quantify early weightlessness-induced changes in cardiovascular function or potential dysfunction. Forty studies of healthy young volunteers (10 men and 10 women, each studied twice) were conducted to determine changes in the sympatho-vagal balance of autonomic control of cardiovascular regulation during graded headward and footward blood volume shifts. Changes in sympatho-vagal balance were classified by changes in the mean levels and spectral content of cardiovascular variables and verified by changes in circulating levels of catecholamines and pancreatic polypeptide. Possible shifts in intra/extravascular fluid were assessed from changes in hematocrit and plasma mass density while changes in the stimulus to regulate plasma volume were determined from Plasma Renin Activity (PRA). Autonomic blockade was used to unmask the relative contribution of sympathetic and parasympathetic efferent influences in response to 10 min each of 0, 20 and 40 mmHg Lower Body Negative Pressure (LBNP) and 15 and 30 mmHg Positive Pressure (LBPP). The combination of muscarinic blockade with graded LBNP and LBPP was used to evoke graded increases and decreases in sympathetic activity without parasympathetic contributions. The combination of beta blockade with graded LBNP and LBPP was used to produce graded increases and decreases in parasympathetic activity without beta sympathetic contributions. Finally, a combination of both beta and muscarinic blockades with LBNP and LBPP was used to determine the contribution from other, primarily alpha adrenergic, sources. Mean values, spectral analyses and time frequency analysis of R-R interval (HR), Arterial Pressure (AP), peripheral blood flow (RF), Stroke Volume (SV) and peripheral resistance (TPR) were performed for all phases of the study. Skin blood Flow (SF) was also measured in other studies and similarly analyzed. Spectra were examined for changes in three frequency regions (low 0.006 - 0.005 Hz (LF), mid 0.05 - 0.15 Hz (W), and high 0.15 - 0.45 Hz (EF)). The primary objective of the study was to indicate which changes in the mean values and/or spectra of cardiovascular variables consistently correlated with changes in sympatho-vagal balance in response to headward and footward fluid shifts. A secondaey objective was to quantify the vascular and extravascular fluid shifts evoked by LBNP and LBPP. The principal hypothesis being tested was that headward fluid shifts would evoke an increase in parasympathetic activity and footward fluid shifts would evoke an increase in sympathetic activity both of which would be detected by spectral analysis and verified by circulating hormones. Hematocrit (HCT), plasma mass density and plasma renin activity increased with muscarinic blockade and with LBNP, a response indicative of a plasma shift to extravascular spaces. Beta blockade alone or after muscarinic blockade had no effect on HCT or plasma mass density. With respect to intravascular fluid volume distribution, LBNP and LBPP produced sufficient upper body vascular fluid shifts to evoke appropriate autonomic regulatory responses.

Knapp, Charles F.↗
DOE OSTI2018

Roles of SDF-1/CXCR4 axis in cartilage endplate stem cells mediated promotion of nucleus pulposus cells proliferation

Highlights: • NPCs proliferation was promoted by co-cultured CESCs and CESCs-CM in vitro. • SDF-1 and CXCR4 were expressed in a complementary pattern in CESCs and NPCs. • CESCs promoted NPCs proliferation partially via SDF-1/CXCR4 axis. • SDF-1/CXCR4 axis mainly activated ERK1/2 pathway rather than Akt pathway in NPCs. • CESCs upregulated TIMP1 expression in NPCs in a SDF-1/CXCR4 axis independent manner. Stem cells transplantation has shown considerable promise in intervertebral disc repair and low-back pain release. Cartilage endplate stem cells (CESCs) also showed potential for nucleus pulposus (NP) regeneration in a rabbit disc degeneration model, the precise mechanism was unclear. Here we investigated the effects of CESCs on NP cells (NPCs) proliferation and the mechanism in vitro. CESCs and NPCs were isolated from surgical specimens of degenerative human lumbar disc. NPCs were co-cultured with CESCs at a 1:1 ratio or cultured in CESCs conditioned medium (CESCs-CM). NPCs proliferation was evaluated by Ki-67 staining, CCK-8 assay and cell cycle analysis. Gene expressions were detected by qRT-PCR and activation of Akt and ERK1/2 was detected by western blot. CXCR4 antagonist AMD3100 was used to block SDF-1/CXCR4 axis. ERK1/2 and Akt inhibitors were used to block Akt and ERK1/2 activation. Results showed that NPCs proliferation was promoted by direct-contact co-culturing with CESCs as well as culturing in CESCs-CM. SDF-1 expression level in CESCs was significantly higher than that in NPCs, while CXCR4 was the opposite. Promotion of NPCs proliferation mediated by CESCs-CM could be partially attenuated by AMD3100. CESCs-CM activated both Akt and ERK1/2 in NPCs, while rhSDF-1 scarcely activated Akt but obviously activated ERK1/2. Akt and ERK1/2 inhibitors could partially inhibited CESCs-CM mediated promotion of NPCs proliferation and showed cumulative effect, while ERK1/2 inhibitor and AMD3100 could significantly abrogate SDF-1 mediated promotion of NPCs proliferation. Our results suggested that CESCs might promote NPCs proliferation in a paracrine pathway, which was partially mediated by SDF-1/CXCR4 axis via ERK1/2 signaling transduction pathway.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2013

Quantitative comparison between PGNAA measurements and MCNP calculations in view of the characterization of radioactive wastes in Germany and France

The Forschungszentrum Juelich GmbH (FZJ), together with the Aachen University Rheinisch-Westfaelische Technische Hochschule (RWTH) and the French Alternative Energies and Atomic Energy Commission (CEA Cadarache) are involved in a cooperation aiming at characterizing toxic and reactive elements in radioactive waste packages by means of Prompt Gamma Neutron Activation Analysis (PGNAA). The French and German waste management agencies have indeed defined acceptability limits concerning these elements in view of their projected geological repositories. A first measurement campaign was performed in the new Prompt Gamma Neutron Activation Analysis (PGNAA) facility called MEDINA, at FZJ, to assess the capture gamma-ray signatures of some elements of interest in large samples up to waste drums with a volume of 200 liter. MEDINA is the acronym for Multi Element Detection based on Instrumental Neutron Activation. This paper presents MCNP calculations of the MEDINA facility and quantitative comparison between measurement and simulation. Passive gamma-ray spectra acquired with a high purity germanium detector and calibration sources are used to qualify the numerical model of the crystal. Active PGNAA spectra of a sodium chloride sample measured with MEDINA then allow for qualifying the global numerical model of the measurement cell. Chlorine indeed constitutes a usual reference with reliable capture gamma-ray production data. The goal is to characterize the entire simulation protocol (geometrical model, nuclear data, and postprocessing tools) which will be used for current measurement interpretation, extrapolation of the performances to other types of waste packages or other applications, as well as for the study of future PGNAA facilities.

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗
DOE OSTI2019

Final Report – Cooperative Research and Development Agreement between National Energy Technology Laboratory and Applied Spectra, Inc.

Groundwater resources can be contaminated by a variety of surface and subsurface sources. For instance, metals can be leached from surface dumps (e.g. landfills, etc.) and reach groundwater supplies by percolation though the soil. Additionally, subsurface activities such as geologic carbon storage (GCS) could result in contamination. In the event of CO2 leakage, freshwater above the injection site can potentially be contaminated with metals either originating from the formation fluids or leached from formation rocks by CO2 acidification. Detection of these contaminating metals is critical for maintaining water safety and providing an early warning of a containment breach. Therefore, a compact, subsurface capable laser induced breakdown spectroscopy (LIBS) based sensor has been developed for groundwater quality monitoring applications. LIBS sensor technology has been advanced from benchtop feasibility studies to a custom fabricated, watertight prototype. The all optical sensor head is built using a low cost passively Q-switched (PQSW) laser, which is fiber coupled to a control unit containing the pump laser and a gated spectrometer. Separating the LIBS excitation laser from the pump and spectrometer allows the expensive and fragile components to remain in a safe, central location while only the sensor head needs to enter the subsurface. Testing of the prototype sensors show Limits of Detection (LODs) comparable to benchtop, actively Q-switched systems and has verified that the fiber coupling and triggering schemes perform as desired.

McIntyre, Dustin↗
DOE OSTI2013

THE DUST TAIL OF ASTEROID (3200) PHAETHON

We report the discovery of a comet-like tail on asteroid (3200) Phaethon when imaged at optical wavelengths near perihelion. In both 2009 and 2012, the tail appears {approx}>350'' (2.5 Multiplication-Sign 10{sup 8} m) in length and extends approximately in the projected anti-solar direction. We interpret the tail as being caused by dust particles accelerated by solar radiation pressure. The sudden appearance and the morphology of the tail indicate that the dust particles are small, with an effective radius {approx}1 {mu}m and a combined mass {approx}3 Multiplication-Sign 10{sup 5} kg. These particles are likely products of thermal fracture and/or desiccation cracking under the very high surface temperatures ({approx}1000 K) experienced by Phaethon at perihelion. The existence of the tail confirms earlier inferences about activity in this body based on the detection of anomalous brightening. Phaethon, the presumed source of the Geminid meteoroids, is still active.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2021

Modeling Data Flows with Network Calculus in Cyber-Physical Systems: Enabling Feature Analysis for Anomaly Detection Applications

The electric grid is becoming increasingly cyber-physical with the addition of smart technologies, new communication interfaces, and automated grid-support functions. Because of this, it is no longer sufficient to only study the physical system dynamics, but the cyber system must also be monitored as well to examine cyber-physical interactions and effects on the overall system. To address this gap for both operational and security needs, cyber-physical situational awareness is needed to monitor the system to detect any faults or malicious activity. Techniques and models to understand the physical system (the power system operation) exist, but methods to study the cyber system are needed, which can assist in understanding how the network traffic and changes to network conditions affect applications such as data analysis, intrusion detection systems (IDS), and anomaly detection. In this paper, we examine and develop models of data flows in communication networks of cyber-physical systems (CPSs) and explore how network calculus can be utilized to develop those models for CPSs, with a focus on anomaly and intrusion detection. This provides a foundation for methods to examine how changes to behavior in the CPS can be modeled and for investigating cyber effects in CPSs in anomaly detection applications.

97 MATHEMATICS AND COMPUTING↗
NASA NTRS2002

Evidence for vestibular regulation of autonomic functions in a mouse genetic model

Physiological responses to changes in the gravitational field and body position, as well as symptoms of patients with anxiety-related disorders, have indicated an interrelationship between vestibular function and stress responses. However, the relative significance of cochlear and vestibular information in autonomic regulation remains unresolved because of the difficulties in distinguishing the relative contributions of other proprioceptive and interoceptive inputs, including vagal and somatic information. To investigate the role of cochlear and vestibular function in central and physiological responses, we have examined the effects of increased gravity in wild-type mice and mice lacking the POU homeodomain transcription factor Brn-3.1 (Brn-3bPou4f3). The only known phenotype of the Brn-3.1(-/-) mouse is related to hearing and balance functions, owing to the failure of cochlear and vestibular hair cells to differentiate properly. Here, we show that normal physiological responses to increased gravity (2G exposure), such as a dramatic drop in body temperature and concomitant circadian adjustment, were completely absent in Brn-3.1(-/-) mice. In line with the lack of autonomic responses, the massive increase in neuronal activity after 2G exposure normally detected in wild-type mice was virtually abolished in Brn-3.1(-/-) mice. Our results suggest that cochlear and vestibular hair cells are the primary regulators of autonomic responses to altered gravity and provide genetic evidence that these cells are sufficient to alter neural activity in regions involved in autonomic and neuroendocrine control.

Non-NASA Center↗
DOE OSTI2013

SOLAR H{alpha} OSCILLATIONS FROM INTENSITY AND DOPPLER OBSERVATIONS

Chromospheric wave activity around flares and filaments has been a research focus for years, and could provide indirect measurements of local conditions that are not otherwise accessible. One interesting observed phenomenon is oscillations in filaments, activated by distant flares and the large-scale waves they produce. Characteristics of these oscillations, such as periods, amplitudes, and lifetimes, can provide unique information about the filament. We measure oscillation properties in flares and filaments from H{alpha} chromospheric data using a new method that provides important spatial and frequency content of the dynamics. We apply the method to two flare events where filaments are observed to oscillate and determine their properties. We find strong oscillatory signal in flaring active regions in the chromosphere over a range of frequencies. Two filaments are found to oscillate without any detectable chromospheric wave acting as an activation mechanism. We find that filaments oscillate with periods of tens of minutes, but variations are significant at small spatial scales along the filamentary region. The results suggest that there is a frequency dependence of the oscillation amplitude, as well as a spatial dependence along single filaments that is more difficult to quantify. It also appears that the strength of the oscillations does not necessarily depend on the strength of the trigger, although there are other possible effects that make this conclusion preliminary. Applications of this technique to other events and different data sets will provide important new insights into the local energy densities and magnetic fields associated with dynamic chromospheric structures.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2018

3D Measurement Simulation and Relative Pointing Error Verification of the Telescope Mount Assembly Subsystem for the Large Synoptic Survey Telescope

An engineering validation of a large optical telescope consists of executing major performing tests at the subsystem level to verify the overall engineering performance of the observatory. Thus, the relative pointing error verification of the telescope mount assembly subsystem is of special interest to guarantee the absolute pointing performance of the large synoptic survey telescope. This paper presents a new verification method for the relative pointing error assessment of the telescope mount assembly, based on laser tracker technology and several fiducial points fixed to the floor. Monte-Carlo-based simulation results show that the presented methodology is fit for purpose, even if floor movement occurs due to temperature variation during the measurement acquisition process. A further research about laser tracker technology integration into the telescope structure may suggest that such laser tracker technology could be permanently installed in the telescope in order to provide an active alignment system that aims to detect and correct possible misalignment between mirrors or to provide the required mirror positioning verification accuracy after maintenance activities. The obtained results show that two on-board laser tracker systems combined with eight measurement targets could result in measurement uncertainties that are better than 1 arcsec, which would provide a reliable built-in metrology tool for large telescopes.

47 OTHER INSTRUMENTATION↗
DOE OSTI2014

Towards the detection of magnetohydrodynamics instabilities in a fusion reactor

Various active control strategies of the Neoclassical tearing modes are being studied in present tokamaks using established detection techniques which exploit the measurements of the fluctuations of the magnetic field and of the electron temperature. The extrapolation of such techniques to the fusion reactor scale is made problematic by the neutron fluence and by the physics conditions related to the high plasma temperature and density which degrade the spatial resolution of such measurements.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗
DOE OSTI2023

Removal of fission product tellurium and iodine from recently irradiated HEU using copper metal

Gamma detection of select fission products or activation products such as 153 Sm and 239 Np in a fission sample is difficult due to gamma interferences from radiotellurium and radioiodine. To aid in the detection of these difficult-to-detect nuclides, the radiotellurium and radioiodine were removed using Cu metal turnings. This method is highly effective at the removal of the interfering radionuclides removing > 98% of the radiotellurium and radioiodine. A mechanistic study was conducted. Determining the deposition mechanism, using a number of techniques including XANES and electrochemistry providing an understanding of the electrochemical path for the Te deposition. Initial work focused on the use of stable Te, further expanding into activated TeO 2 , and a fresh fission sample. Importantly, this method presents a rapid method for the direct detection of select fission products that could have a significant impact on nuclear related fields including nuclear forensics.

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEA↗
DOE OSTI2012

GAMMA-RAY LOUDNESS, SYNCHROTRON PEAK FREQUENCY, AND PARSEC-SCALE PROPERTIES OF BLAZARS DETECTED BY THE FERMI LARGE AREA TELESCOPE

The parsec-scale radio properties of 232 active galactic nuclei, most of which are blazars, detected by the Large Area Telescope (LAT) on board the Fermi Gamma-ray Space Telescope have been observed contemporaneously by the Very Long Baseline Array (VLBA) at 5 GHz. Data from both the first 11 months (1FGL) and the first 2 years (2FGL) of the Fermi mission were used to investigate these sources' {gamma}-ray properties. We use the ratio of the {gamma}-ray-to-radio luminosity as a measure of {gamma}-ray loudness. We investigate the relationship of several radio properties to {gamma}-ray loudness and to the synchrotron peak frequency. There is a tentative correlation between {gamma}-ray loudness and synchrotron peak frequency for BL Lac objects in both 1FGL and 2FGL, and for flat-spectrum radio quasars (FSRQs) in 2FGL. We find that the apparent opening angle tentatively correlates with {gamma}-ray loudness for FSRQs, but only when we use the 2FGL data. We also find that the total VLBA flux density correlates with the synchrotron peak frequency for BL Lac objects and FSRQs. The core brightness temperature also correlates with synchrotron peak frequency, but only for the BL Lac objects. The low-synchrotron-peaked (LSP) BL Lac object sample shows indications of contamination by FSRQs which happen to have undetectable emission lines. There is evidence that the LSP BL Lac objects are more strongly beamed than the rest of the BL Lac object population.

79 ASTRONOMY AND ASTROPHYSICS↗