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At least 1,117 records · Page 62
All sourcesNASA NTRSDOE OSTI
NASA NTRS2016

The NANOGrav Nine-Year Data Set: Limits on the Isotropic Stochastic Gravitational Wave Background

We compute upper limits on the nanohertz-frequency isotropic stochastic gravitational wave background (GWB) using the 9 year data set from the North American Nanohertz Observatory for Gravitational Waves (NANOGrav) collaboration. Well-tested Bayesian techniques are used to set upper limits on the dimensionless strain amplitude (at a frequency of 1 yr(exp -1) for a GWB from supermassive black hole binaries of A(sub gw) less than 1.5 x 10(exp -15). We also parameterize the GWB spectrum with a broken power-law model by placing priors on the strain amplitude derived from simulations of Sesana and McWilliams et al. Using Bayesian model selection we find that the data favor a broken power law to a pure power law with odds ratios of 2.2 and 22 to one for the Sesana and McWilliams prior models, respectively. Using the broken power-law analysis we construct posterior distributions on environmental factors that drive the binary to the GW-driven regime including the stellar mass density for stellar-scattering, mass accretion rate for circumbinary disk interaction, and orbital eccentricity for eccentric binaries, marking the first time that the shape of the GWB spectrum has been used to make astrophysical inferences. Returning to a power-law model, we place stringent limits on the energy density of relic GWs, OMEGA(sub gw) (f) h squared less than 4.2 x 10(exp -10). Our limit on the cosmic string GWB, OMEGA(sub gw) (f) h squared less than 2.2 x 10(exp -10), translates to a conservative limit on the cosmic string tension with G mu less than 3.3 x 10(exp -8), a factor of four better than the joint Planck and high-l‚ cosmic microwave background data from other experiments.

Arzoumanian, Z.↗
DOE OSTI2024

Abstract for CRADA between Capture6 CORP and NETL

The National Energy Technology Laboratory (NETL) and Capture6 CORP (Participant) will collaborate to refine Capture6’s transformative air contactor design for optimal direct air capture (DAC) under an awarded project from the Department of Energy’s Direct Air Capture Pre-Commercialization Technology Prize. Current cooling tower designs require exponentially more resources as the concentration of CO 2 in the gas phase decreases, resulting in a low conversion rate of carbonates, a large physical footprint, and high evaporation of water, which consequently limits the energy and water benefits that a DAC process can create. Capture6’s innovative tower design AURA (Absorption Unit Reactor for Ambient air) seeks to improve the mass transfer characteristics of legacy cooling towers and reduces total energy demand by: (1) introducing laminar and turbulent flow to increase mass transfer, (2) generating a solvent gradient to improve CO 2 absorption, (3) leveraging venturi and packed towers to increase the surface for the mass-transfer process, (4) adding charged/cool plates to reduce overall water loss in the system, and (5) utilizing surfactant additives to increase mass transfer. The collaboration will facilitate the deployment of technologies that reduce the total energy required and carbon footprint of solvent-based carbon dioxide removal (CDR) technologies. The anticipated results support NETL’s goal of fostering rapid development and commercialization pathways for technologies that remove CO 2 from the atmosphere.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗
NASA NTRS1996

Recycling crop residues for use in recirculating hydroponic crop production

As part of bioregenerative life support feasibility testing by NASA, crop residues are being used to resupply elemental nutrients to recirculating hydroponic crop production systems. Methods for recovering nutrients from crop residues have evolved from water soaking (leaching) to rapid aerobic bioreactor processing. Leaching residues recovered the majority of elements but it also recovered significant amounts of soluble organics. The high organic content of leachates was detrimental to plant growth. Aerobic bioreactor processing reduced the organic content ten-fold, which reduced or eliminated phytotoxic effects. Wheat and potato production studies were successful using effluents from reactors having with 8- to 1-day retention times. Aerobic bioreactor effluents supplied at least half of the crops elemental mass needs in these studies. Descriptions of leachate and effluent mineral content, biomass productivity, microbial activity, and nutrient budgets for potato and wheat are presented.

NASA Center KSC↗
DOE OSTI2023

Survival, healing, and swim performance of juvenile migratory sea lamprey ( Petromyzon marinus ) implanted with a new acoustic microtransmitter designed for small eel-like fishes

Background: Little is known about the transformer stage of the parasitic lampreys, a brief but critical period that encompasses juvenile out-migration from rivers to lakes or oceans to begin parasitic feeding. Information about this life stage could have significant conservation implications for both imperiled and invasive lampreys. We investigated tag retention, survival, wound healing, and swim performance of newly transformed sea lamprey (Petromyzon marinus) implanted with a new micro-acoustic transmitter, the eel–lamprey acoustic transmitter (ELAT), in a controlled laboratory environment. Results: The 61-day survival of our tagged subjects was 71%, within the range reported in similar studies of juvenile lampreys. However, survival was significantly lower in the tagged animals (vs control), with no effect statistically attributable to measures of animal length, mass, condition, or population of origin (Great Lakes vs. Atlantic drainage). Mortality in tagged fish was concentrated in the first four days post-surgery, suggesting injury from the surgical process. An unusually long recovery time from anesthesia may have contributed to the increased mortality. In a simple burst swim assay, tagged animals swam significantly slower (– 22.5%) than untagged animals, but were not significantly different in endurance swim tests. A composite wound healing score at day four was a significant predictor of maximum burst swim speed at day 20, and wound condition was related to animal mass, but not length, at the time of tagging. Conclusions: Impairments to survival and swim performance of juvenile sea lamprey implanted with the ELAT transmitter were within currently reported ranges for telemetry studies with small, difficult to observe fishes. Our results could be improved with more refined anesthesia and surgical techniques. The ability to track migratory movements of imperiled and pest populations of parasitic lampreys will improve our ability to estimate vital rates that underlie recruitment to the adult population (growth, survival) and to investigate the environmental factors that regulate the timing and rates of movement, in wild populations.

59 BASIC BIOLOGICAL SCIENCES↗
NASA NTRS1988

The dc power control for a liquid-fed resistojet

A simple breadboard power controller was designed and demonstrated for a new liquid-fed water resistojet. The 1-piece laboratory model thruster has an integrated vaporizer/superheater using a single heating element. Heater temperature was maintained at or near a preset reference value with the closed loop controller providing pulse width modulated (PWM) dc power into the thruster heater. A combined thruster, temperature readout, PWM transfer function was experimentally determined. This transfer function was used to design a proportional plus integral controller that demonstrated zero steady state error, conservative stability margins and adequate transient response to step changes in propellant flow rate, input voltage and temperature reference. Initial turn-on temperature overshoot from room temperature to a 650 C setpoint was 80 C. In addition, EMI was alleviated by reducing heater dI/dt and dV/dt using a simple diode-inductor-capacitor network. Based on limited initial tests, thruster preheat with no propellant flow was necessary to achieve stable system operation during startup. Breadboard power efficiency was 99 percent at 1 kW, and component mass was 0.4 kg excluding the power loss and mass of an input filter required for spacecraft integration.

Gruber, Robert P.↗
NASA NTRS1988

dc power control for a liquid-fed resistojet

A simple breadboard power controller was designed and demonstrated for a new liquid-fed water resistojet. The 1-piece laboratory model thruster has an integrated vaporizer/superheater using a single heating element. Heater temperature was maintained at or near a preset reference value with the closed loop controller providing pulse width modulated (PWM) dc power into the thruster heater. A combined thruster, temperature readout, PWM transfer function was experimentally determined. This transfer function was used to design a proportional plus integral controller that demonstrated zero steady state error, conservative stability margins and adequate transient response to step changes in propellant flow rate, input voltage and temperature reference. Initial turn-on temperature overshoot from room temperature to a 650 C setpoint was 80 C. In addition, EMI was alleviated by reducing heater dI/dt and dV/dt using a simple diode-inductor-capacitor network. Based on limited initial tests, thruster preheat with no propellant flow was necessary to achieve stable system operation during startup. Breadboard power efficiency was 99 percent at 1 kW, and component mass was 0.4 kg excluding the power loss and mass of an input filter required for spacecraft integration.

Gruber, Robert P.↗
DOE OSTI2023

Dose Rate Effects on Degradation of Nuclear Power Plant Electrical Cable Insulation at a Common Dose

The intent of this report is to address an identified knowledge gap in relating accelerated aging of nuclear electrical cables to service aging: dose rate effects (DRE). Here, DRE refer to gamma radiation-induced polymer degradation being a function of dose rate in addition to total absorbed dose. The concern raised is that historical qualification conducted at higher dose rates to simulate service lifetime may underestimate insulation degradation that occurs at lower dose rates in service. In the work described herein, common nuclear cable insulation materials—cross-linked polyethylene (XLPE) and ethylene propylene diene elastomer (EPDM)—were subjected to accelerated aging at ambient temperature (26°C) at different gamma dose rates of 100, 200 and 1800 Gy/h for select exposure durations to achieve constant total doses of 170, 210 and 300 kGy to evaluate DRE. First, the cable insulation material types investigated are described. Then, the accelerated aging experimental process involving gamma irradiation applied to the insulation specimens at room temperature and different dose rates is discussed. Then, the experimental characterization techniques used to perform this work are elucidated. These include elongation at break (EAB), mass change, yellowness index (YI), carbonyl index (CI), density, indenter modulus (IM), and relaxation constant (t). Theory of polymer degradation is discussed, and characterization results and discussion are provided. Finally, concluding remarks are made. The findings from this work and cited prior work reveal that DRE are material dependent, even between similar material categories (e.g., XLPE). In the case of the EPDM studied, degradation of ductility was observed to be greater at higher dose rate for the same total dose, indicating accelerated gamma aging to be more conservative than extended aging. Thus, conclusions regarding the conservatism of historical qualification likely require additional consideration for specific materials and conditions in question. The results of this study support the contention that, due to inherent limitations and uncertainties associated with prediction of cable remaining useful life from accelerated aging experiments, trending of installed cable insulation health status will be more practical and useful for safe and efficient cable aging management repair and replace decisions than lifetime prediction from historical qualification. The combination of material robustness demonstrated by the qualification process and ongoing monitoring of cable health status combine to provide confidence in continued safe use of existing nuclear cables. Additional research into effective and efficient condition monitoring methods for non-destructive evaluation of installed cables is needed to support aging cable management, including material studies to inform interpretation of measured results.

21 SPECIFIC NUCLEAR REACTORS AND ASSOCIATED PLANTS↗
NASA NTRSDate not supplied

Integrated Bosch Process System Models for In-Situ Oxygen and Carbon Production

In-Situ Resource Utilization (ISRU) technology is a vital component to NASA’s mission of a sustainable presence on the Moon and Mars. Local resources can be leveraged to reduce resupply frequency and mass. Elements of the Bosch process, combined with the carbothermal reduction process, can produce oxygen on the lunar surface with minimal consumables. The Bosch process can also produce oxygen on the Martian surface by using the CO 2 -rich environment. Between both systems, adsorption pump, solar thermal energy, carbon formation reactor, and water recovery subsystems are modeled and integrated to create a functional model in MATLAB software. The model is used to simulate performance of the system and reduce mass, power, and volume requirements. This integrated system model provides a tool to scale ISRU technologies for oxygen and carbon production. The MATLAB model is created by developing a system of independent subsystem models that are solved for their quasi-steady state values which can be integrated with respect to time to determine the change in current states. A flexible time stepping method is used to ensure a high level of accuracy during periods of rapid change while still making use of a simple explicit integration method. The flexible time step is calculated for each independent subsystem and the minimum value from those is used as the overall time step. A flexible time step is calculated by dividing a resolution value, or the maximum change per time step, by the variables current rate of change. The maximum value from all points in space is used for subsystem models that contain multiple values. The process is done for every variable that is being monitored in each subsystem and the global minimum is used as that iteration’s timestep. Several assumptions used in the MATLAB model for fluid flow dynamics, such as 1-D gas flow through the sorption pump, are supported by modeling in Ansys Fluent software. The Lunar oxygen production system is outlined in Fig. 1. The carbothermal reduction subsystem uses solar energy to heat a mixture of lunar regolith and carbon powder to produce carbon monoxide. To begin, the carbon monoxide feeds to the modified Bosch subsystem along with hydrogen gas. The reactants then enter the carbon formation reactor where water and carbon powder are produced. Solar thermal energy is used to add energy to the reactor, but waste heat from the carbothermal process is another potential heat source. The water is collected and electrolyzed to produce hydrogen which reenters the Bosch subsystem, and the oxygen is stored for downstream use. The carbon powder is collected and feeds back into the carbothermal subsystem. The Martian oxygen production system uses the full Bosch process and is outlined in Fig 2. A CO 2 adsorption pump thermally cycles to scrub and pressurize CO 2 from the environment. Along with an initial supply of hydrogen, the reactants enter the Reverse Water Gas Shift Reactor (RWGSR) which produces carbon monoxide and water. Carbon monoxide and unreacted hydrogen enter the carbon formation reactor to produce water and carbon powder. The water is collected from both reactors and electrolyzed to reintroduce hydrogen and store oxygen for propellant production or life support. Carbon is removed from the carbon formation reactor and stored. The adsorption pump utilizes rapid cycle temperature swings within a stack of zeolite coated surfaces. The subsystem model solves 1-D quasi-steady conservation laws of the quasi-steady form, shown in Eq. 1, for the gas stream and heat exchange liquid to predict performance parameters such as breakthrough capacity and optimum cycle time. The source term S is used to capture interactions between the fluid flows and the sorbent. A quasi-steady-state scheme is used where no time derivatives appear in the governing equations, except for those in the source terms. This results in an autonomous system, where ∂F/∂x = ƒ(F). The fluxes F are provided at the inlet, and an explicit method is used to solve for the spatial distribution of F. The heat and mass flows to the sorbent are then extracted from the source terms. These flows are numerically integrated to produce a 1-D solution for the system’s state as a function of both time and space. The body of the adsorption pump is separated into two semi-independent models: the heat exchanger fluid flow and gas flow through the zeolite coated surfaces. Both models are solved using the above-described method to find a 1-D solution as a function of space and interact only once a timestep is taken. The interaction point is the sorbent through which all heat transfer between the two models must occur. Sorbent mass adsorption is calculated using the Lagergren model, shown in Eq. 2, where the transfer coefficient, λ D , is found by solving a system of nondimensionalized equations derived by using the heat and mass transfer analogy for transport phenomena. Using Grade 544 Type 13X zeolite as the sorbent material, the equilibrium concentration, θ eq , is calculated using the k-site Langmuir isotherm and fit parameters. Additionally, the enthalpy of adsorption used in the model is computed by interpolation of available data [1]. The subsystem model was validated using the Rapid Cycle Temperature Swing Adsorption (RC-TSA) pump. The solar thermal energy subsystem focuses on a solar concentrator concept with a heat exchanger to heat the reactants before entering the carbon formation reactor. The subsystem model assumes a fixed solar flux and reflector efficiency to calculate the reactant temperature given the incoming temperature, pressure, and exchanger geometry. The receiver is a custom manufactured series of copper blocks with serpentine channels to increase its surface area and the residence time of the reactants to heat up to 550 °C. The subsystem model was validated using a heat exchanger developed at NASA Glenn Research Center (GRC). The solar thermal energy subsystem focuses on a solar concentrator concept with a heat exchanger to heat the reactants before entering the carbon formation reactor. The subsystem model assumes a fixed solar flux and reflector efficiency to calculate the reactant temperature given the incoming temperature, pressure, and exchanger geometry. The receiver is a custom manufactured series of copper blocks with serpentine channels to increase its surface area and the residence time of the reactants to heat up to 550 °C. The subsystem model was validated using a heat exchanger developed at NASA Glenn Research Center (GRC).

In situ Resource Utilization↗
NASA NTRS2013

Resolution of Forces and Strain Measurements from an Acoustic Ground Test

The Conservatism in Typical Vibration Tests was Demonstrated: Vibration test at component level produced conservative force reactions by approximately a factor of 4 (approx.12 dB) as compared to the integrated acoustic test in 2 out of 3 axes. Reaction Forces Estimated at the Base of Equipment Using a Finite Element Based Method were Validated: FEM based estimate of interface forces may be adequate to guide development of vibration test criteria with less conservatism. Element Forces Estimated in Secondary Structure Struts were Validated: Finite element approach provided best estimate of axial strut forces in frequency range below 200 Hz where a rigid lumped mass assumption for the entire electronics box was valid. Models with enough fidelity to represent diminishing apparent mass of equipment are better suited for estimating force reactions across the frequency range. Forward Work: Demonstrate the reduction in conservatism provided by; Current force limited approach and an FEM guided approach. Validate proposed CMS approach to estimate coupled response from uncoupled system characteristics for vibroacoustics.

Smith, Andrew M.↗
NASA NTRS1999

Orbit Determination for Mars Global Surveyor During Mapping

The Mars Global Surveyor (MGS) spacecraft reached a low-altitude circular orbit on February 4, 1999, after the termination of the second phase of aerobraking. The MGS spacecraft carries the Mars Orbiter Laser Altimeter (MOLA) whose primary goal is to derive a global, geodetically referenced 0.2 deg x 0.2 deg topographic grid of Mars with a vertical accuracy of better than 30 meters. During the interim science orbits in the' Hiatus mission phase (October - November 1997), and the Science Phasing Orbits (March - April, 1998, and June - July 1998) 208 passes of altimeter data were collected by the MOLA instrument. On March 1, 1999 the first ten orbits of MOLA altimeter data from the near-circular orbit were successfully returned from MGS by the Deep Space Network (DSN). Data will be collected from MOLA throughout the Mapping phase of the MCS mission, or for at least one Mars year (687 days). Whereas the interim orbits of Hiatus and SPO were highly eccentric, and altimeter data were only collected near periapsis when the spacecraft was below 785 km, the Mapping orbit of MGS is near circular, and altimeter data will be collected continuously at a rate of 10 Hz. The proper analysis of the altimeter data requires that the orbit of the MGS spacecraft be known to an accuracy comparable to that of the quality of the altimeter data. The altimeter has an ultimate precision of 30 cm on mostly flat surfaces, so ideally the orbits of the MGS spacecraft should be known to this level. This is a stringent requirement, and more realistic goals of orbit error for MGS are ten to thirty meters. In this paper we will discuss the force and measurement modelling required to achieve this objective. Issues in force modelling include the proper modelling of the gravity field of Mars, and the modelling of non-conservatives forces, including the development of a 'macro-model', in a similar fashion to TOPEX/POSEIDON and TDRSS. During Cruise and Aerobraking, the high gain antenna (HGA) was stowed on the +X face of the spacecraft. On March 29, 1999 the HGA will be deployed on a meter long boom which will remain Earth-pointing while the instrument panel (including the MOLA instrument) remains pointed at nadir. The tracking data must be corrected for the regular motion of the high gain antenna with respect to the center of mass, and the success of the MGS determination during Mapping will depend on correctly accounting for this offset in the measurement model.

Lemoine, F. G.↗
NASA NTRS1993

Conservative force model performance for TOPEX/Poseidon precision orbit determination

The TOPEX/Poseidon spacecraft was launched on August 10, 1992 to study the Earth's oceans. To achieve maximum benefit from the altimetric data collected, mission requirements dictate that TOPEX/Poseidon's orbit must be computed at an unprecedented level of accuracy. In order to satisfy these requirements, a model which accounts for the satellite's complex geometry, attitude, and surface properties has been developed. This `box-wing' representation treats the spacecraft as the combination of flat plates arranged in the shape of a box and a connecetd solar array. The nonconservative forces acting on each of the eight surfaces are computed independently, yielding vector accelerations which are summed to compute the total aggregate effect on the satellite center-of-mass. Parameters associated with each flat plate were derived from a finite element analysis of the spacecraft. Certain parameters can be inferred from tracking data and have been adjusted to obtain a better representation of the satellite acceleration history. Changes in the nominal mission profile and the presence of an `anomalistic' force have complicated this tuning process. Model performance, parameter sensitivities, and the `anomalistic' force will be discussed.

Marshall, J. Andrew↗
DOE OSTI2021

Astrophysical Explosions Revisited: Collisionless Coupling of Debris to Magnetized Plasma

The coupling between a rapidly expanding cloud of ionized debris and an ambient magnetized plasma is revisited with a hybrid (kinetic ion/fluid electron) simulation code that allows a study over a wide range of plasma parameters. Over a specified range of hypothetical conditions, simple scaling laws in terms of the total debris mass and explosion speed are derived and verified for the maximal size of the debris cloud and the fraction of debris that free-streams from the burst along the magnetic field. Furthermore, the amount of debris that escapes from the burst with minimal coupling to the background magnetic field increases with the debris gyroradius. Test cases with two different debris species—including a heavy minority species with a relatively large gyroradius—highlight how the collisionless coupling of the debris depends on the single particle trajectories as well as the overall conservation of energy and momentum.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2020

A molecular switch regulating transcriptional repression and activation of PPARγ

Nuclear receptor (NR) transcription factors use a conserved activation function-2 (AF-2) helix 12 mechanism for agonist-induced coactivator interaction and NR transcriptional activation. In contrast, ligand-induced corepressor-dependent NR repression appears to occur through structurally diverse mechanisms. We report two crystal structures of peroxisome proliferator-activated receptor gamma (PPARγ) in an inverse agonist/corepressor-bound transcriptionally repressive conformation. Helix 12 is displaced from the solvent-exposed active conformation and occupies the orthosteric ligand-binding pocket enabled by a conformational change that doubles the pocket volume. Paramagnetic relaxation enhancement (PRE) NMR and chemical crosslinking mass spectrometry confirm the repressive helix 12 conformation. PRE NMR also defines the mechanism of action of the corepressor-selective inverse agonist T0070907, and reveals that apo-helix 12 exchanges between transcriptionally active and repressive conformations—supporting a fundamental hypothesis in the NR field that helix 12 exchanges between transcriptionally active and repressive conformations.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2026

Getting Warmer: IceCube Nears Freeze Out

IceCube has recently detected a diffuse population of high-energy neutrinos arising from the Milky Way. We use this high-significance detection to place new limits on dark matter (DM) annihilation to neutrinos with two complementary approaches. The first method uses the background-subtracted Galactic longitude distribution of shower events to place a conservative bound on the DM annihilation cross section that does not rely on any assumed Galactic cosmic ray emission model; the resulting limits on the velocity-averaged annihilation cross section improve upon existing bounds by factors of a few. The second method uses the template-dependent neutrino energy spectra from the Inner Galaxy, inferred under different Galactic cosmic ray emission models. This complementary approach shows that the inferred Galactic neutrino intensities are already sensitive to DM contributions near the thermal-relic benchmark for a range of TeV-scale DM masses, though this comparison is more model-dependent. Our results demonstrate that measurements of diffuse Galactic neutrino emission can be used as a powerful probe of DM annihilation into neutrinos. Future observations with IceCube-Gen2 and KM3NeT will substantially extend this sensitivity, potentially allowing a decisive test of the thermal freeze-out mechanism with Galactic neutrino observations.

Mukhopadhyay, Mainak [Fermilab; Chicago U., KICP; ↗
DOE OSTI2022

Design optimization of an additively manufactured prototype recuperator for supercritical CO 2 power cycles

Supercritical CO 2 (sCO 2 ) power cycles are being developed due to their potential for high efficiency and reduced capital cost. It is necessary that these recuperators operate at high pressures and temperatures, up to 30 MPa and 900 K, with effectiveness values > 95% and pressure drops <1% to achieve high cycle efficiencies. Moreover, it is also necessary to have reasonable cost recuperators to control the capital costs of the sCO 2 power cycles. In this study, a Plate Pin-Fin (PPF) heat exchanger has been proposed as an sCO 2 recuperator. This preliminary recuperator design leverages capabilities enabled by additive manufacturing. Although the PPF design has characteristics similar to those of a plate heat exchanger, small diameter and relatively long fins are used to increase surface area, enhance heat transfer, and provide structural support for the partition plates that separate the fluid streams. Existing correlations for heat transfer and pressure drop were adapted for the PPF heat exchanger. These correlations were implemented in a 1D analytical model and used for the optimization of a 5-kW th high temperature recuperator for an indirect sCO 2 cycle by varying the design parameters to minimize the quantity of material required. A 3D conjugate heat transfer numerical simulations were conducted to validate the heat transfer and pressure loss correlations. A steepest descent method was used to minimize heat exchanger mass for a 5-kW prototype recuperator subject to a maximum specified pressure drop. Additionally, the design analysis indicated that an optimum PPF recuperator would be attained for the minimum allowable pin transverse spacing, minimum pin width, minimum pin height and near maximum cell aspect ratio. Moreover, at a low material requirement of 0.216 kg/kW and a pressure drop, which is almost five times lower than the allowable pressure drop design target, the optimized PPF heat exchanger has the high potential to be an alternative to a printed circuit heat exchanger, which is a conservative design basis for the current state-of-the-art sCO 2 recuperators.

42 ENGINEERING↗
DOE OSTI2021

Structure and Function of REP34 Implicates Carboxypeptidase Activity in Francisella tularensis Host Cell Invasion

Francisella tularensis is the etiological agent of tularemia, or rabbit fever. Although F. tularensis is a recognized biothreat agent with broad and expanding geographical range, its mechanism of infection and environmental persistence remain poorly understood. Previously, we identified seven F. tularensis proteins that induce a rapid encystment phenotype (REP) in the free-living amoeba, Acanthamoeba castellanii. Encystment is essential to the pathogen's long term intracellular survival in the amoeba. Here, we characterize the cellular and molecular function of REP34, a REP protein with a mass of 34 kDa. A REP34 knock-out strain of F. tularensis has a reduced ability to both induce encystment in A. castellanii and invade human macrophages. We determined the crystal structure of REP34 to 2.05-Å resolution and demonstrate robust carboxypeptidase B-like activity for the enzyme. REP34 is a zinc-containing monomeric protein with close structural homology to the metallocarboxypeptidase family of peptidases. REP34 possesses a novel topology and substrate binding pocket that deviates from the canonical funnelin structure of carboxypeptidases, putatively resulting in a catalytic role for a conserved tyrosine and distinct S1' recognition site. Taken together, these results identify REP34 as an active carboxypeptidase, implicate the enzyme as a potential key F. tularensis effector protein, and may help elucidate a mechanistic understanding of F. tularensis infection of phagocytic cells.

59 BASIC BIOLOGICAL SCIENCES↗