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All sourcesNASA NTRSDOE OSTI
DOE OSTI2025

Woody Plant Transformation: Current Status, Challenges, and Future Perspectives

Woody plants, comprising forest and fruit tree species, provide essential ecological and economic benefits to society. Their genetic improvement is challenging due to long generation intervals and high heterozygosity. Genetic transformation, which combines targeted DNA delivery with plant regeneration from transformed cells, offers a powerful alternative to accelerating their domestication and improvement. Agrobacterium tumefaciens, Rhizobium rhizogenes, and particle bombardment have been widely used for DNA delivery into a wide variety of explants, including leaves, stems, hypocotyls, roots, and embryos, with regeneration occurring via direct organogenesis, callus-mediated organogenesis, somatic embryogenesis, or hairy root formation. Despite successes, conventional approaches are hampered by low efficiency, genotype dependency, and a reliance on challenging tissue culture. This review provides a critical analysis of the current landscape in woody plant transformation, moving beyond a simple summary of techniques to evaluate the co-evolution of established platforms with disruptive technologies. Key advances among these include the use of developmental regulators to engineer regeneration, the rise in in planta systems to bypass tissue culture, and the imperative for DNA-free genome editing to meet regulatory and public expectations. By examining species-specific breakthroughs in key genera, including Populus, Malus, Citrus, and Pinus, this review highlights a paradigm shift from empirical optimization towards rational, predictable engineering of woody plants for a sustainable future.

Agrobacterium tumefaciens↗
DOE OSTI2022

DNS of Hydrodynamic Instabilities of Laminar H2/O2/N2 Flames at Elevated Pressure

The atypical combustion properties of hydrogen have long provided an interest for hydrogen-enriched combustion, emphasized in recent years by increasingly stringent emission regulations. Significant work has been focused on the small hydrocarbon(C1-C3)/hydrogen blends, but much less is known about the effect of hydrogen on heavier hydrocarbon combustion, of interest for the aviation and automotive industry. In this work, we perform direct numerical simulations (DNS) of spherically expanding laminar n-dodecane/H2 flames in a constant-volume vessel at elevated pressure and a range of hydrogen seeding levels. A low Mach number model is used to represent the gas and flame dynamics allowing for a temporally varying, spatially homogeneous pressure field, while also enabling numerical evolution of the system numerically at time step sizes governed by the advective CFL limit rather than acoustic processes. We use an adaptive mesh refinement (AMR) approach to tackle the large separation of scales associated with high pressure premixed flame surfaces propagating in a laboratory-scale closed domain. We incorporate detailed chemistry and transport models for the reacting flow and focus the analysis on the effects of the pressure rise on the flame propagation characteristics and morphology as the hydrogen content is increased. The presence of fast diffusive H2 and the pressure rise results in dramatic thinning of the flame and the potential onset of hydrodyanmic and theromdiffusive instabilities. We assess the potential role of these instabilities on the ability to accurately measure laminar burning speed experimentally based on the mean flame surface propagation speed.

high pressure↗
DOE OSTI2024

Stress interference in multilayer additive friction stir deposition of AA6061 aluminum

Due to the multilayer deposition nature of metal additive manufacturing processes, each layer being printed experiences the state of thermokinetic and thermomechanical stress that in turn interfere with the state of thermokinetics and thermomechanical stress of subsequently deposited layers. Especially, this multilayer interference significantly affects the resultant properties of the component fabricated using solid-state additive friction stir deposition due to evolution of asymmetric state of planar stress. Due to the lack of comprehensive and suitable in situ diagnosis technique, the complex interference of inter- and multi-layer stresses during additive friction stir deposition was studied in an integrated approach of numerical simulation of fluidic state and experimental probing of stress influenced ultrasonic elastography. The uni-directional and bi-directional layer deposition configurations adopted during additive friction stir deposition result in the generation of constructive and destructive interference of the interlayer stress and hence, asymmetric and symmetric dynamic elasticity distribution respectively within the subsequent layers. With subsequent deposition of additional layers, the odd and even numbers of deposited layers generate asymmetric and nearly symmetric dynamic elasticity distributions.

Yang, Teng↗
DOE OSTI2020

Physical, numerical, and computational challenges of modeling neutrino transport in core-collapse supernovae

The proposal that core collapse supernovae are neutrino driven is still the subject of active investigation more than 50 years after the seminal paper by Colgate and White. The modern version of this paradigm, which we owe to Wilson, proposes that the supernova shock wave is powered by neutrino heating, mediated by the absorption of electron-flavor neutrinos and antineutrinos emanating from the proto-neutron star surface, or neutrinosphere. Neutrino weak interactions with the stellar core fluid, the theory of which is still evolving, are flavor and energy dependent. The associated neutrino mean free paths extend over many orders of magnitude and are never always small relative to the stellar core radius. Thus, neutrinos are never always fluid like. Instead, a kinetic description of them in terms of distribution functions that determine the number density of neutrinos in the six-dimensional phase space of position, direction, and energy, for both neutrinos and antineutrinos of each flavor, or in terms of angular moments of these neutrino distributions that instead provide neutrino number densities in the four-dimensional phase-space subspace of position and energy, is needed. In turn, the computational challenge is twofold: (i) to map the kinetic equations governing the evolution of these distributions or moments onto discrete representations that are stable, accurate, and, perhaps most important, respect physical laws such as conservation of lepton number and energy and the Fermi–Dirac nature of neutrinos and (ii) to develop efficient, supercomputer-architecture-aware solution methods for the resultant nonlinear algebraic equations. In this review, we present the current state of the art in attempts to meet this challenge.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2022

Oxygen vacancy-rich amorphous FeNi hydroxide nanoclusters as an efficient electrocatalyst for water oxidation

Here, a one-pot strategy is presented to directly synthesize amorphous Fe x Ni y hydroxide nanoclusters (denoted as ANC-Fe x Ni y , <2 nm) with oxygen vacancies induced by ionic liquids. The ANC-Fe x Ni y catalyst presents abundant catalytic sites and high intrinsic conductivity. As such, the optimized ANC-Fe 1 Ni 2 exhibits high activity in oxygen evolution reaction (OER) with a Tafel slope of 39 mV dec –1 and an overpotential of 266 mV at 10 mA cm –2 . Notably, the optimized ANC-Fe 1 Ni 2 shows an extraordinarily large mass activity of 3028 A g FeNi –1 at the overpotential of 300 mV, which is ~24-fold of commercial RuO 2 catalyst. The superior activity of these Fe x Ni y hydroxide nanoclusters is ascribed to (i) the amorphous and distorted structure with abundant oxygen vacancies, and (ii) enhanced active site density by downsizing the ANC-Fe x Ni y clusters. This strategy provides a novel route for enhancing OER electrocatalytic performance and highly encouraging for the future application of amorphous metal hydroxides in catalysis.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
DOE OSTI2026

Advancing Porous Carbons: Understanding the Importance of Surface Chemistry for the Energy–Environment Nexus

This review intends, in a critical way, the comprehensive view of the importance of porous carbons surface chemistry for their applications in an energy− environment nexus. Surface chemistry is presented as a combination of functional heteroatom-containing groups, dopants, and structural defects. First, we briefly address carbon surface chemical environment and the methods of its modification and characterization, indicating their practical limitations. Then, the effects of surface chemistry on separation, catalysis, energy storage, sensing and microwave absorption are introduced. Besides a critical analysis of published findings on these topics, we also include our views on the advancement in the processes which rely on porous carbons surface chemistry, and identify strategic areas and directions that should deserve further attention. We focus on new findings and important original contributions to the field. Since the community of carbon researchers grows following the strategic application of these materials, the role of functional groups, dopants and structural defects in various cutting-edge applications is emphasized, showing the progress in the field and the evolution of findings. A clear determination of the effects of carbon surface is often a challenge since carbons porosity and the locations of specific bonds/sites/ defects in the carbon texture provide nanoconfinement effects.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
DOE OSTI2026

Functionalized Metal–Organic Framework Thin Films for Stable and Efficient Electrochemical Water Oxidation under Near-Neutral Conditions

The development of molecularly modified metal− organic frameworks (MOFs) for electrochemical water oxidation has emerged as a promising strategy for efficient artificial photosynthesis. In this study, a ruthenium-based water oxidation catalyst (WOC) was incorporated into the UiO-67 framework, forming RuM−UiO-67 films grown directly on conductive FTO substrates. These modified films demonstrate efficient water oxidation activity at near-neutral pH (pH 6), operating at a low overpotential of ∼600 mV. The catalyst exhibits a turnover frequency (TOF) of (0.32 ± 0.02) s −1 at 1.5 V versus the normal hydrogen electrode (NHE) in buffered solution (pH 6) for the oxygen evolution reaction. Notably, incorporation into the MOF results in a 12-fold increase in electroactive surface coverage compared to a monolayer of the same catalyst on bare FTO. Faradaic efficiency analysis revealed incomplete conversion to O 2 , and follow-up iodometric analysis confirmed the formation of H 2 O 2 as a competing two-electron oxidation product during electrocatalysis. These results highlight the utility of MOF-based architectures for maximizing the catalyst accessibility and stability under electrochemical water oxidation conditions.

catalysts↗
DOE OSTI2023

Incommensurate charge-stripe correlations in the kagome superconductor CsV 3 Sb 5–x Sn x

The class of AV 3 Sb 5 (A=K, Rb, Cs) kagome metals hosts unconventional charge density wave states seemingly intertwined with their low temperature superconducting phases. The nature of the coupling between these two states and the potential presence of nearby, competing charge instabilities however remain open questions. This phenomenology is strikingly highlighted by the formation of two ‘domes’ in the superconducting transition temperature upon hole-doping CsV 3 Sb 5 . Here we track the evolution of charge correlations upon the suppression of long-range charge density wave order in the first dome and into the second of the hole-doped kagome superconductor CsV 3 Sb 5–x Sn x . Initially, hole-doping drives interlayer charge correlations to become short-ranged with their periodicity diminished along the interlayer direction. Beyond the peak of the first superconducting dome, the parent charge density wave state vanishes and incommensurate, quasi-1D charge correlations are stabilized in its place. These competing, unidirectional charge correlations demonstrate an inherent electronic rotational symmetry breaking in CsV 3 Sb 5 , and reveal a complex landscape of charge correlations within its electronic phase diagram. Our data suggest an inherent 2k ƒ charge instability and competing charge orders in the AV 3 Sb 5 class of kagome superconductors.

36 MATERIALS SCIENCE↗
DOE OSTI2022

Star formation and AGN feedback in the local Universe: Combining LOFAR and MaNGA

The effect of active galactic nuclei (AGN) on their host galaxies – in particular their levels of star formation – remains one of the key outstanding questions of galaxy evolution. Successful cosmological models of galaxy evolution require a fraction of energy released by an AGN to be redistributed into the interstellar medium to reproduce the observed stellar mass and luminosity function and to prevent the formation of over-massive galaxies. Observations have confirmed that the radio-AGN population is energetically capable of heating and redistributing gas at all phases, however, direct evidence of AGN enhancing or quenching star formation remains rare. With modern, deep radio surveys and large integral field spectroscopy (IFS) surveys, we can detect fainter synchrotron emission from AGN jets and accurately probe the star-forming properties of galaxies, respectively. Here, in this paper, we combine data from the LOw Frequency ARray Two-meter Sky Survey (LoTSS) with data from one of the largest optical IFS surveys, Mapping Nearby Galaxies at Apache Point Observatory (MaNGA), to probe the star-forming properties of 307 local (z < 0.15) galaxies that host radio-detected AGN (RDAGN). We compare our results to a robust control sample of non-active galaxies that each match the stellar mass, redshift, visual morphology, and inclination of a RDAGN host. We find that RDAGN and control galaxies have broad star-formation rate (SFR) distributions, typically lie below the SFMS, and have negative stellar light-weighted age gradients. These results indicate that AGN selected based on their current activity are not responsible for suppressing their host galaxies’ star formation. Rather, our results support the maintenance mode role that radio AGN are expected to have in the local Universe.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2021

Reconstructing quintessence

We present an Effective Field Theory based reconstruction of quintessence models of dark energy directly from cosmological data. We show that current cosmological data possess enough constraining power to test several quintessence model properties for redshifts z ϵ [0, 1.5] with no assumptions about the behavior of the scalar field potential. We use measurements of the cosmic microwave background, supernovae distances, and the clustering and lensing of galaxies to constrain the evolution of the dark energy equation of state, Swampland Conjectures, the shape of the scalar field reconstructed potential, and the structure of its phase space. The standard cosmological model still remains favored by data and, within quintessence models, deviations from its expansion history are bounded to be below the 10% level at 95% confidence at any redshift below z = 1.5.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2020

Analytical Investigation of the Role of Lateral Mixing in the Evolution of Nonprecipitating Cumulus. Part I: Developing Clouds

Evolution of nonprecipitating cumulus clouds (Cu) at the developing stage under the influence of lateral entrainment and mixing is studied analytically using a minimalistic analytical model. We present a model of an ascending cloud volume (a model of developing Cu) whose structure is determined by the processes of droplet diffusion growth/evaporation and entrainment mixing in the horizontal direction. Spatial and time changes of liquid water content, the adiabatic fraction, droplet concentration, and the mean volume droplet radius are calculated. It is shown that the existence of a nondiluted core in a growing cumulus cloud significantly depends on the cloud width and vertical velocity. While at the updraft velocity of 2 m s −1 the core of a 400-m-wide cloud becomes diluted at distances of a few hundred meters above cloud base, the core of a cloud of 1000-m width remains nondiluted at distances up to 1500 m above cloud base. The explanation of this result is simple: the increase in cloud width and the decrease in the updraft velocity increase the time during which the cloud is diluted due to mixing. Since lateral mixing synchronously decreases both the cloud water content and droplet concentration, the variation of the mean volume droplet radius is low inside the cloud. The approximate quantitative condition for cloud formation in updraft is derived. It is shown that a cloud can arise when its vertical velocity exceeds a critical value. To produce clouds, narrow turbulent plumes should ascend at higher velocity as compared to wider plumes. High humidity of the environment air is favorable for formation of clouds from plumes. The comparison of the obtained results with previously published observational data indicates a reasonable agreement. The results can be useful for parameterization purposes.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2026

Precision Kinematic Sunyaev--Zel'dovich Measurements Across Halo Mass and Redshift with DESI DR2 and ACT DR6: Part I. Luminous Red Galaxies

We present the most precise measurements of the kinetic Sunyaev-Zel'dovich (kSZ) effect around luminous red galaxies to date, detecting the signal at $18σ$ significance in both harmonic and configuration space. Our analysis cross-correlates 2.4 million spectroscopic LRGs from the Dark Energy Spectroscopic Instrument (DESI) DR2 sample with Data Release 6 (DR6) of the Atacama Cosmology Telescope (ACT). We develop a novel harmonic-space cross-correlation approach using momentum-weighted kSZ templates, yielding nearly uncorrelated bandpowers within a framework consistent with other large-scale structure analyses. By incorporating the LRG halo occupation distribution (HOD) and its uncertainty, we convert measured galaxy gas profiles into halo gas profiles and provide generalized Navarro-Frenk-White (GNFW) fitting profiles, providing empirical targets for tuning feedback efficiency in hydrodynamical simulations and for baryonic modeling in large-scale structure analyses. We find strong evidence that gas profiles do not trace dark matter, providing direct evidence for gas redistribution beyond gravitational collapse. Comparing to hydrodynamical simulations, our measurements favor feedback efficiencies exceeding those in the Battaglia profile, suggesting more efficient gas ejection in group-scale halos than previously predicted. Splitting by redshift, we detect the kSZ signal at SNR $\approx 5$--$10$ in each of four bins and find amplitude evolution consistent with the expected decline in mean halo mass at fixed comoving number density. Splitting by stellar mass, we study the scaling of kSZ amplitude with galaxy properties. Together with BGS and ELG measurements in Paper II, these results span $0.1 \lesssim z \lesssim 1.6$ across three galaxy populations, demonstrating the potential of spectroscopic kSZ to map circumgalactic gas and constrain baryonic feedback.

Qu, F. J. [KIPAC, Menlo Park; Stanford U., HEPL; S↗
DOE OSTI2023

A comparative study of solute trapping in Fe-(33–45 at%) Cu alloys manufactured by laser directed energy deposition

The high cooling rates in laser directed energy deposition (DED-LB) of alloys lead to substantial amounts of solute trapping as solute atoms cannot diffuse away from the solid/liquid interface before it advances. In some concentrated alloys, this results in supersaturated solid phases that form nanoscale hierarchical microstructures when the solute atoms precipitate out during reheating from subsequent laser passes. We choose the Iron-Copper (Fe-Cu) binary alloy as model system as it is chemically homogeneous in the liquid phase and has negligible solid solubility at room temperature. Two alloys with nominal compositions in atomic (at.) %, Fe67Cu33 and Fe55Cu45, were manufactured using DED-LB. Scanning transmission electron microscopy (STEM), energy dispersive spectroscopy (EDS) and wavelength dispersive spectroscopy (WDS) were used to characterize the nanostructures and heterogeneous chemical compositions. A non-equilibrium solute partitioning model was used to compute the supersaturated chemistries of the constituent phases and validated with experimentally measured compositions. The measured phase compositions of the two alloys were very similar, at roughly 12 at.% Cu and 4 at.% Fe in the α(bcc)-Fe and ε(fcc)-Cu phases respectively, despite having different processing parameters and mechanical behavior. This indicates that the total thermal history, that depends on both the processing parameters and laser scan pattern, plays a stronger role on the final microstructure evolution than just the initial quantity of trapped solute. In addition, we find that current non-equilibrium solute partitioning models applied on the continuum scale fall short of predicting accurate quantitative phase compositions in concentrated alloys, although the qualitative trends are captured correctly.

36 MATERIALS SCIENCE↗
DOE OSTI2026

ODIN: Confirmation and 3D Reconstruction of Six Massive Protoclusters at Cosmic Noon

Protoclusters represent sites of accelerated galaxy formation and extreme astrophysical activity characteristic of dense environments. Identifying massive protoclusters and mapping their spatial structures are therefore crucial for understanding how large-scale environment influences galaxy evolution. We combine wide-field Lyα imaging from the One-hundred-deg$^{2}$ DECam Imaging in Narrowbands survey with extensive Dark Energy Spectroscopic Instrument and ancillary spectroscopy across the extended COSMOS and XMM Large Scale Structure (LSS) fields (≈14 deg$^{2}$) to search for massive protoclusters. We confirm six systems at z ≈ 2.4 and ≈ 3.1, including three newly identified structures and three which overlap with previously known structures and/or systems detected using other tracers. We reconstruct their three-dimensional structures, estimate descendant halo masses, and for one structure at z ≈ 3.12, demonstrate that overlapping narrowband filters (NB497 and N501) provide accurate redshift tomography for emission-line galaxies. One protocluster at z ≈ 2.45 overlaps with one of the LATIS tomographic fields, enabling direct comparison between galaxy and H i overdensities traced by Lyα forest absorption. Another at z ≈ 3.12 hosts a massive quiescent galaxy (M$_{*}$ ≈ 1.2 × 10$^{11}$M$_{⊙}$), suggesting that overdense environments may play a role in accelerating galaxy assembly and quenching. Comparing Lyα emission properties across environments, we find that protocluster galaxies exhibit higher median line fluxes and a deficit of faint emitters relative to the field. The effect is strongest when combining 2D and 3D density information, indicating that galaxies in the densest protocluster cores are most affected by environmental processes. This effect is stronger at z ≈ 3.1 than at z ≈ 2.4, suggesting possible redshift evolution.

Ortiz, Ashley [Purdue U.] (ORCID:000900083184304X)↗
DOE OSTI2022

Structure Evolution of Chemically Degraded ZIF-8

Exposure of the metal–organic zeolitic imidazolate framework-8 (ZIF-8) to humid SO x gas cleaves Zn–N bonds, leading to framework disorder and a corresponding decrease in pore accessibility. This work shows that framework disorder exhibits a critical behavior with respect to water concentration in the gas stream. While the quantity of sulfate adsorbed at Zn nodes is at or below the detection threshold for the X-ray pair distribution function technique, precluding direct observation of the adsorbate, powder diffraction reveals clear structural alterations. Here, the onset of linker cleavage correlates to a large microstrain that diminishes as framework disorder increases. Energy-dispersive X-ray spectroscopy shows that the sulfur distribution throughout representative grains is uniform, implying the structure transformation is homogeneously nucleated and not dependent upon gas diffusion kinetics.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
DOE OSTI2023

Noble gas insights into early impact delivery and volcanic outgassing to Earth's atmosphere: A limited role for the continental crust

Earth's atmosphere, crust and mantle have evolved together through continuous geochemical exchange throughout Earth's history. Constraints on the transport of volatile elements and compounds between these reservoirs are crucial for understanding how Earth could have stayed habitable for extended periods of time. In this work, we present a new forward model of He, Ne and Ar in the mantle, crust and atmosphere. We explore concentrations of noble gases at the end of accretion, bulk silicate Earth K/U ratios, crustal growth scenarios, and upper mantle processing rates throughout Earth's history. We search for parameter combinations that simultaneously satisfy observational constraints on present-day mantle 4 He/ 3 He and 40 Ar/ 36 Ar (sensitive to mantle outgassing and continental crust growth, which depletes the mantle of U, Th and K), atmospheric 20 Ne/ 22 Ne (which tracks the mix of outgassed vs. delivered Ne in the atmosphere), and atmospheric 40 Ar/ 36 Ar in the past and today (sensitive to volatile delivery, mantle outgassing and crustal growth). Leveraging this intertwined set of noble gas abundances and isotopic compositions yields new constraints on initial noble gas abundances in the mantle and on the proportions of atmospheric volatiles originating from delivery by impact degassing, mantle outgassing, and degassing of the continental crust. We find that atmospheric Ar isotopic evolution is primarily sensitive to the mantle processing rate history; the atmospheric Ar isotopic record should therefore not be used to reconstruct continental crust growth, but instead provides valuable insights into mantle processing rates. Our model predicts a measurably low 20 Ne/ 22 Ne ratio of ~9.7 in Archean atmospheric samples. Most of atmospheric primordial 36 Ar was directly delivered by chondritic bodies and not transferred to the atmosphere during an intense early episode of mantle outgassing. Nitrogen delivered by impact degassing could account for the present-day atmospheric nitrogen inventory.

58 GEOSCIENCES↗
DOE OSTI2025

Experimental platforms for investigating feature-driven jets for HED mix model validation

High-energy-density (HED) systems, such as inertial confinement fusion (ICF), are susceptible to hydrodynamic instabilities that can significantly affect both experimental results and modeling predictions. Isolated features, such as fill tubes or divots in the capsule, can cause material to jet as a result of the compressive shock exciting the Richtmyer–Meshkov instability, and serve as one of the primary degradation mechanisms in ICF yield. Simulations of feature-driven jets and how they mix require extensive experimental validation, particularly for understanding to what degree the initial size and shape of a feature influence jet dynamics, and how much instability feeds through downstream layers. A better understanding of feature-driven jetting can improve our mix modeling capabilities and increase hydrodynamic simulation accuracy. This manuscript describes a series of experimental platforms fielded by Los Alamos National Laboratory as a part of the Mshock Omega 60 and ModCons Omega EP campaigns to explore feature-driven jetting. These platforms are designed to benchmark jet evolution and growth as a function of initial feature size and shape, investigate jet-layer interactions leading to instability feedthrough, and will be used to characterize jet-jet interactions resulting from clusters of features. In conclusion, preliminary results for both platforms are shown. The ModCons experiments are on-going, and a discussion of future work directions is included.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗