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At least 1,099 records · Page 61

Singleplex, multiplex and pooled sample real-time RT-PCR assays for detection of SARS-CoV-2 in an occupational medicine setting

Abstract For workplaces which cannot operate as telework or remotely, there is a critical need for routine occupational SARS-CoV-2 diagnostic testing. Although diagnostic tests including the CDC 2019-Novel Coronavirus (2019-nCoV) Real-Time RT-PCR Diagnostic Panel (CDC Diagnostic Panel) (EUA200001) were made available early in the pandemic, resource scarcity and high demand for reagents and equipment necessitated priority of symptomatic patients. There is a clearly defined need for flexible testing methodologies and strategies with rapid turnaround of results for (1) symptomatic, (2) asymptomatic with high-risk exposures and (3) asymptomatic populations without preexisting conditions for routine screening to address the needs of an on-site work force. We developed a distinct SARS-CoV-2 diagnostic assay based on the original CDC Diagnostic Panel (EUA200001), yet, with minimum overlap for currently employed reagents to eliminate direct competition for limited resources. As the pandemic progressed with testing loads increasing, we modified the assay to include 5-sample pooling and amplicon target multiplexing. Analytical sensitivity of the pooled and multiplexed assays was rigorously tested with contrived positive samples in realistic patient backgrounds. Assay performance was determined with clinical samples previously assessed with an FDA authorized assay. Throughout the pandemic we successfully tested symptomatic, known contact and travelers within our occupational population with a ~ 24–48-h turnaround time to limit the spread of COVID-19 in the workplace. Our singleplex assay had a detection limit of 31.25 copies per reaction. The three-color multiplexed assay maintained similar sensitivity to the singleplex assay, while tripling the throughput. The pooling assay further increased the throughput to five-fold the singleplex assay, albeit with a subtle loss of sensitivity. We subsequently developed a hybrid ‘multiplex-pooled’ strategy to testing to address the need for both rapid analysis of samples from personnel at high risk of COVID infection and routine screening. Herein, our SARS-CoV-2 assays specifically address the needs of occupational healthcare for both rapid analysis of personnel at high-risk of infection and routine screening that is essential for controlling COVID-19 disease transmission. In addition to SARS-CoV-2 and COVID-19, this work demonstrates successful flexible assays developments and deployments with implications for emerging highly transmissible diseases and future pandemics.

60 APPLIED LIFE SCIENCES↗

Kilauea Magnetotelluric Dataset

In 2002 and 2003 a collaborative effort was undertaken between Lawrence Berkeley National Laboratory, Sandia National Laboratories, the USGS Menlo Park, the USGS Hawaiian Volcano Observatory, and Electromagnetic Instruments Inc. to study the Kilauea volcano in Hawaii using the magnetotelluric (MT) technique. The work was motivated by a desire to improve understanding of the magma reservoirs and conduits within Kilauea and the East and Southwest Rift zones, which has implications for understanding Kilaueas plumbing system. An improved understanding of the rift zones has implications in understanding large-scale landslides that are generated in the Hilina Slump, which produce significant impacts on coastal communities. Up to eight stations operated simultaneously, with multiple remote reference sites, and data were processed using multi-station robust processing techniques. In total, data were acquired at 70 sites over the Southwest and East rift zones. Good to excellent quality data were obtained even in the harshest conditions, such as those encountered on the fresh lava flows of the East Rift Zone (ERZ), where electrical contact resistances are on the order of 100 kOhm. This data supports the continuing efforts to increase geothermal power on the island of Hawaii. Each of the 70 EDI files are the MT impedance tensors for 1 site. There is also a description of the processing of the data and a site map showing the locations of each site.

15 GEOTHERMAL ENERGY↗

Continuously variable voltage-controlled phase shifter

Phase shifter circuit adjusts the phase relationship between a locally generated reference frequency and a received RF signal applied to a phase-coherent detector. It is small enough to be integrated into a receiver subassembly and operates on command from remote control panels.

Johns, C. E.↗

Introductory comments on the USGS geographic applications program

The third phase of remote sensing technologies and potentials applied to the operations of the U.S. Geological Survey is introduced. Remote sensing data with multidisciplinary spatial data from traditional sources is combined with geographic theory and techniques of environmental modeling. These combined imputs are subject to four sequential activities that involve: (1) thermatic mapping of land use and environmental factors; (2) the dynamics of change detection; (3) environmental surveillance to identify sudden changes and general trends; and (4) preparation of statistical model and analytical reports. Geography program functions, products, clients, and goals are presented in graphical form, along with aircraft photo missions, geography test sites, and FY-70.

Gerlach, A. C.↗

Shear strength of lunar soil from Oceanus Procellarum.

Soil from the scoop of Surveyor 3, returned to earth by Apollo 12 astronauts, has been tested in a miniature shear box at five bulk densities, from 0.99 to 1.87 g/cu cm. Cohesion increased with bulk density from .03 to .3 N/sq cm; internal friction angle increased from 13 to 56 deg. Shear stress vs normal stress data fit a logarithmic relationship better than a linear one, at normal stresses of .003 to 3 N/sq cm. Results of these tests, in air, show no systematic differences from those for tests made elsewhere in vacuum and nitrogen. Results agree with those obtained in remotely controlled lunar surface operations with Surveyor 3 and other spacecraft provided that the bulk density was slightly underestimated for the on-surface measurements.

Jaffe, L. D.↗

ASSESS program: Shuttle Spacelab simulation using a Lear jet aircraft (mission no. 2)

The second shuttle Spacelab simulation mission of the ASSESS program was conducted at Ames Research Center by the Airborne Science Office (ASO) using a Lear jet aircraft based at a site remote from normal flight operations. Two experimenters and the copilot were confined to quarters on the site during the mission, departing only to do in-flight research in infrared astronomy. A total of seven flights were made in a period of 4 days. Results show that experimenters with relatively little flight experience can plan and carry out a successful research effort under isolated and physically rigorous conditions, much as would more experienced scientists. Perhaps the margin of success is not as great, but the primary goal of sustained acquisition of significant data over a 5-day period can be achieved.

Reller, J. O., Jr.↗

Nimbus 4 IRIS spectra in the 750-1250 wavelengths/cm atmospheric window region

Present operational schemes for infrared remote sounding measurements of surface temperature use the 899 wavelengths/cm atmospheric window region. Spectra from the Nimbus 4 IRIS in the 750 to 1250 wavelengths/cm region are analyzed. Comparison of the actual surface temperature and the observed brightness temperature at 10 wavelengths/cm resolution shows that the clearest windows were at 936 and 960 wavelengths/cm. Although there is a small amount of CO2 absorption in these regions, this is compensated for by a decrease in water vapor continuum absorption. Atmospheric absorption was 0.5 K less than experienced by the 899 wavelengths/cm window.

Kunde, V. G.↗

A study of application of remote sensing to river forecasting. Volume 1: Executive summary

A project is described whose goal was to define, implement and evaluate a pilot demonstration test to show the practicability of applying remotely sensed data to operational river forecasting in gaged or previously ungaged watersheds. A secondary objective was to provide NASA with documentation describing the computer programs that comprise the streamflow forecasting simulation model used. A computer-based simulation model was adapted to a streamflow forecasting application and implemented in an IBM System/360 Model 44 computer, operating in a dedicated mode, with operator interactive control through a Model 2250 keyboard/graphic CRT terminal. The test site whose hydrologic behavior was simulated is a small basin (365 square kilometers) designated Town Creek near Geraldine, Alabama.

Source record↗

Coast Guard/NOAA/NASA Great Lakes Project Icewarn

The operational feasibility of using remote sensing to provide all weather ice formation for Great Lakes winter navigation is explored. A combination airborne pulsed radar system to measure actual ice thickness, a satellite data link system, and a hand drawn interpretive ice chart proved valuable for extending winter navigation through the icepack.

Brennan, T. D.↗

Analysis and design of a capsule landing system and surface vehicle control system for Mars exploration

Problems related to the design and control of an autonomous rover for the purpose of unmanned exploration of the planets were considered. Building on the basis of prior studies, a four wheeled rover of unusual mobility and maneuverability was further refined and tested under both laboratory and field conditions. A second major effort was made to develop autonomous guidance. Path selection systems capable of dealing with relatively formidable hazard and terrains involving various short range (1.0-3.0 meters), hazard detection systems using a triangulation detection concept were simulated and evaluated. The mechanical/electronic systems required to implement such a scheme were constructed and tested. These systems include: laser transmitter, photodetectors, the necessary data handling/controlling systems and a scanning mast. In addition, a telemetry system to interface the vehicle, the off-board computer and a remote control module for operator intervention were developed. Software for the autonomous control concept was written. All of the systems required for complete autonomous control were shown to be satisfactory except for that portion of the software relating to the handling of interrupt commands.

Gisser, D. G.↗

An underwater work systems package

A modular unit which is adaptable to several existing deep sea submersibles was developed to extend their working abilities and acquire knowledge of components and techniques for working in the deep sea environment. This work systems package is composed of an aluminum pipe structure on which are mounted two six-function grabber arms, a seven function manipulator, tool suit, 1,000/lb. capacity winch, electrohydraulic power supply, electronics housing, lights, and television. The unit is designed to be operated by itself either remotely or with divers, attached to manned submersibles, or mounted on unmanned cable-controlled submersibles.

Estabrook, N. B.↗

Summary of the active microwave users workshop

A coordinated microwave applications development program was initiated to improve the capability to: (1) identify, monitor, and assess the earth's resources; and (2) monitor the earth's environment and predict significant changes. The program consists of the scientific, technical, and programmatic activities required to develop microwave remote sensing into an operational tool for systematic earth observations.

Source record↗

Annual Symposium on Machine Processing of Remotely Sensed Data, 4th, Purdue University, West Lafayette, Ind., June 21-23, 1977, Proceedings

Papers are presented on a variety of techniques for the machine processing of remotely sensed data. Consideration is given to preprocessing methods such as the correction of Landsat data for the effects of haze, sun angle, and reflectance and to the maximum likelihood estimation of signature transformation algorithm. Several applications of machine processing to agriculture are identified. Various types of processing systems are discussed such as ground-data processing/support systems for sensor systems and the transfer of remotely sensed data to operational systems. The application of machine processing to hydrology, geology, and land-use mapping is outlined. Data analysis is considered with reference to several types of classification methods and systems.

Morrison, D. B.↗

Excavation costs for lunar materials

A lunar strip mining system is presented which is capable of excavating and transporting 3 million metric tons of ore per year to a central processing plant on the moon's surface. The mining system would grow from a single front-end loader in the first year, to a fleet of ten haulers in the 30th year. Lunar personnel requirements would consist of a single individual, whose primary function would be to perform maintenance. All of the mining equipment would either operate automatically or by remote control from earth. The projected cost for the lunar mining system is approximately $12 to $37 per ton of ore over the life of the mine, an important part of the overall economics of exploiting lunar resources.

Carrier, W. D., III↗

Remote sensing applications to resource problems in South Dakota

Cooperative projects between RSI and numerous South Dakota agencies have provided a means of incorporating remote sensing techniques into operational programs. Eight projects discussed in detail are: (1) detection of high moisture zones near interstate 90; (2) thermal infrared census of Canada geese in South Dakota; (3) dutch elm disease detection in urban environment; (4) a feasibility study for monitoring effective precipitation in South Dakota using TIROS-N; (5) open and abandoned dump sites in Spink county; (6) the influence of soil reflectance on LANDSAT signatures of crops; (7) A model implementation program for Lake Herman watershed; and (8) the Six-Mile Creek investigation follow-on.

Myers, V. I.↗

Optical Fiber Illumination System for visual flight simulation

An electronically controlled lighting system simulating runway, aircraft carrier, and landing aid lights for flight simulations is described. The various colored lights that would be visible to a pilot by day, at dusk, or at night are duplicated at the distances the lights would normally become visible. Plastic optical fiber illuminators using tungsten halogen lights are distributed behind the model. The tips of the fibers of illuminators simulating runway lights are bevelled in order that they may be seen from long distances and at low angles. Fibers representing taxiway lights are pointed and polished for omni-directional visibility. The electronic intensity controls, which can be operated either manually or remotely, regulate the intensity of the lights to simulate changes in distance. A dichronic mirror, infrared filter system is used to maintain color integrity.

Hollow, R. H.↗

Estimation of dynamic stability parameters from drop model flight tests

The overall remotely piloted drop model operation, descriptions, instrumentation, launch and recovery operations, piloting concept, and parameter identification methods are discussed. Static and dynamic stability derivatives were obtained for an angle attack range from -20 deg to 53 deg. It is indicated that the variations of the estimates with angle of attack are consistent for most of the static derivatives, and the effects of configuration modifications to the model were apparent in the static derivative estimates.

Chambers, J. R.↗

Motorized Cyrogenic Valve

Remotely-controlled cryogenic valve operates over temperature range from room temperature to 2 K. Valve used in helium dilution refrigerators, cryostats and adiabatic-demagnetization refrigerators.

Salerno, L.↗