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At least 1,099 records · Page 61
All sourcesNASA NTRSDOE OSTI
DOE OSTI2024

Packaging “vegetable oils”: Insights into plant lipid droplet proteins

Abstract Plant neutral lipids, also known as “vegetable oils”, are synthesized within the endoplasmic reticulum (ER) membrane and packaged into subcellular compartments called lipid droplets (LDs) for stable storage in the cytoplasm. The biogenesis, modulation, and degradation of cytoplasmic LDs in plant cells are orchestrated by a variety of proteins localized to the ER, LDs, and peroxisomes. Recent studies of these LD-related proteins have greatly advanced our understanding of LDs not only as steady oil depots in seeds but also as dynamic cell organelles involved in numerous physiological processes in different tissues and developmental stages of plants. In the past 2 decades, technology advances in proteomics, transcriptomics, genome sequencing, cellular imaging and protein structural modeling have markedly expanded the inventory of LD-related proteins, provided unprecedented structural and functional insights into the protein machinery modulating LDs in plant cells, and shed new light on the functions of LDs in nonseed plant tissues as well as in unicellular algae. Here, we review critical advances in revealing new LD proteins in various plant tissues, point out structural and mechanistic insights into key proteins in LD biogenesis and dynamic modulation, and discuss future perspectives on bridging our knowledge gaps in plant LD biology.

Cai, Yingqi (ORCID:0000000203575809)↗
DOE OSTI2024

Towards rational control of seed oil composition: dissecting cellular organization and flux control of lipid metabolism

Plant lipids represent a fascinating field of scientific study, in part due to a stark dichotomy in the limited fatty acid (FA) composition of cellular membrane lipids vs the huge diversity of FAs that can accumulate in triacylglycerols (TAGs), the main component of seed storage oils. With few exceptions, the strict chemical, structural, and biophysical roles imposed on membrane lipids since the dawn of life have constrained their FA composition to predominantly lengths of 16–18 carbons and containing 0–3 methylene-interrupted carbon-carbon double bonds in cis-configuration. However, over 450 “unusual” FA structures can be found in seed oils of different plants, and we are just beginning to understand the metabolic mechanisms required to produce and maintain this dichotomy. Here we review the current state of plant lipid research, specifically addressing the knowledge gaps in membrane and storage lipid synthesis from 3 angles: pathway fluxes including newly discovered TAG remodeling, key acyltransferase substrate selectivities, and the possible roles of “metabolons.”

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2025

Complete replacement of Arabidopsis oil-producing enzymes with heterologous diacylglycerol acyltransferases

Acyl-CoA:diacylglycerol acyltransferase 1 (DGAT1) and phospholipid:diacylglycerol acyltransferase 1 (PDAT1) share responsibility for triacylglycerol (TAG) biosynthesis, and their selectivities control TAG fatty acid (FA) compositions. For rational metabolic engineering of seed oils, replacing endogenous TAG biosynthesis with exogenous enzymes containing different substrate FA selectivities is desirable; however, the dgat1-1/pdat1-2 double mutant is pollen lethal. Here, we evaluated the ability of 3 DGAT1s, from phylogenetically diverse plants with distinct TAG assembly processes, to completely replace endogenous TAG biosynthesis in Arabidopsis ( Arabidopsis thaliana ). We transformed dgat1-1 mutant plants with expression constructs for DGAT1 s from Camelina sativa , Physaria fendleri , and castor ( Ricinus communis ). Transgene expression was properly “contextualized” by using a previously determined minimum necessary expression unit containing the promoter/5′ UTR and first intron of native AtDGAT1 ; both of these DNA elements are essential for pollen expression. Next, we crossed homozygous lines with a DGAT1/DGAT1/PDAT1/pdat1-2 parent. C. sativa and P. fendleri DGAT1s restored the FA compositions and transcriptional differences of dgat1-1 to near wild-type and rescued the dgat1-1/pdat1-2 pollen lethality. R. communis DGAT1 was active in dgat1-1 seeds but produced unique oil profiles and alterations in the expression of lipid metabolic genes; it also failed to rescue dgat1-1/pdat1-2 lethality. This study confirms that the promoter and first intron of AtDGAT1 can modulate the expression of foreign DGAT1 genes to fit the correct spatiotemporal profile necessary for completely replacing endogenous TAG biosynthesis. Furthermore, it demonstrates an additional layer of unexpected enzyme incompatibility between oilseed lineages, which may complicate bioengineering approaches that seek to replace essential genes with orthologs.

McGuire, Sean T. [Washington State Univ., Pullman,↗
DOE OSTI2026

Metabolic flux, metabolite, and transcript analysis uncover reprogramming of metabolism toward higher seed oil

Overexpression of WRINKLED1 (WRI1), a master regulator of glycolysis and fatty acid biosynthesis, together with DIACYLGLYCEROL ACYLTRANSFERASE1 (DGAT1), which catalyzes the final step of triacylglycerol assembly, is a promising strategy for enhancing seed oil content. However, how these regulators coordinate system-wide metabolic reprogramming at the levels of gene expression, metabolite pools, and fluxes remains poorly understood. To address this, we performed 13 C-metabolic flux analysis, metabolomics, and transcriptomics on in vitro cultured pennycress (Thlaspi arvense L.) embryos overexpressing the native WRI1 and DGAT1 homologs. Here, in cultured embryos, WRI1/DGAT1 overexpression increased triacylglycerol accumulation by 28% while reducing protein content by 34%, relative to the wild type. Embryos showed ∼20-fold and 50-fold upregulation of WRI1 and DGAT1 along with induction of WRI1 target genes in glycolysis and fatty acid biosynthesis. Genes associated with photosynthesis and Calvin cycle functions were also upregulated, whereas genes encoding ribosomal proteins and seed storage proteins were strongly repressed, consistent with the observed lipid–protein tradeoff. Flux analysis revealed that enhanced triacylglycerol biosynthesis is supported by increased flux through the Rubisco shunt and cytosolic pyruvate kinase, while the oxidative pentose phosphate pathway and malic enzyme contributed little to NADPH or pyruvate supply. Metabolomic profiling revealed extensive perturbations in glycolytic intermediates, tricarboxylic acid cycle metabolites, and amino acids. In plant grown seeds, WRI1/DGAT1 lines also showed a modest but significant increase in total lipid content. Collectively, these findings reveal how WRI1 and DGAT1 reprogram central metabolism to enhance oil accumulation, with relevance to mature seeds.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2016

Quality of goat′s milk cheese as affected by coating with edible chitosan‐essential oil films

The effectiveness of applying chitosan coatings containing rosemary and oregano essential oils to cheeses was analysed. Cheeses were coated by immersing the samples two or three times successively in the different formulations. The ripening indexes, water loss, fungal growth and sensory properties of the coated and noncoated cheeses throughout ripening were evaluated. The coatings both prevented weight loss and improved the microbial safety. The lipolytic and proteolytic activities were reduced in coated cheese, which is in line with the antimicrobial effect of active coatings. Sensory evaluation revealed that the cheeses double‐coated with chitosan–oregano oil were the best evaluated in terms of aroma and flavour.

Cano Embuena, Amalia Isabel↗
DOE OSTI2021

Identification and analysis of stem‐specific promoters from sugarcane and energy cane for oil accumulation in their stems

Abstract Considerable recent progress has been achieved in bioengineering oil accumulation in the vegetative tissues of plants, opening an opportunity for large scale production of biodiesel, jet fuel, lubricants, and high‐value lipid bioproducts. For the highly productive C4 crops, such as sugarcane, energy cane, Miscanthus, and fiber sorghums, the bulk of the biomass is the stem. However, little success has been made in accumulating oil in the stem. Since engineering a trait with a constitutive promoter often results in pleiotropic effects that counter trait improvement, identification of stem parenchyma‐specific promoters is a prerequisite for efficient use of the ample photoassimilates stored in mature stem parenchyma cells. In this study, we first identified two TST genes encoding homologues of tonoplast sugar transporters that were strongly and almost exclusively expressed in the stems of canes via a combination of RNA‐seq atlas analysis, in silico analysis of a sugarcane genome, phylogenetic analysis, and quantitative PCR analysis. They were further confirmed in the pith parenchyma cells of the mature stem by RNA in situ hybridization. When fused with the β‐Glucuronidase (GUS) reporter gene, the promoters of two alleles, TST2b‐1A and TST2b‐1C , from one TST gene demonstrated that they could drive the GUS expression exclusively in the stem in Arabidopsis.

09 BIOMASS FUELS↗
DOE OSTI2019

Interactive effects of dietary fishmeal level and plant essential oils supplementation on European sea bass, Dicentrarchus labrax : Growth performance, nutrient utilization, and immunological response

Abstract The inclusion of phytogenics in fish feed is a promising strategy to compensate for the negative performance effects of replacing fishmeal (FM) with vegetable sources. The present work assessed the interactive effects of different dietary FM levels (22.5 and 10% of formulation) and the supplementation of a commercial blend of anise, citrus, and oregano essential oils (Digestarom PEP M.G.E 150) on European sea bass, Dicentrarchus labrax growth performance, nutrient utilization, gut morphology, antioxidant status, and immunological response over a 60‐day growth trial. Results showed decreased growth and protein efficiency ratio and increased feed conversion ratio with a low dietary FM level. In contrast, supplementation of the phytogenic product demonstrated improved performance and nutrient utilization together with increased protein and energy retention. Supplementation with the plant essential oils fully compensated for the negative intestinal changes observed in sea bass fed a low‐FM diet but showed little improvement in fish immunological response, except for the 30% increase in lysozyme activity observed in fish fed the low FM‐supplemented diet compared to those fed the standard high‐FM diet. Overall, this study supports the use of this phytogenic product in low‐FM diets as a possible tool to decrease feed costs associated with FM without compromising fish performance, nutrient utilization, and health.

Gonçalves, Rui A.↗
DOE OSTI2024

Targeted modulation of pennycress lipid droplet proteins impacts droplet morphology and seed oil content

SUMMARY Lipid droplets (LDs) are unusual organelles that have a phospholipid monolayer surface and a hydrophobic matrix. In oilseeds, this matrix is nearly always composed of triacylglycerols (TGs) for efficient storage of carbon and energy. Various proteins play a role in their assembly, stability and turnover, and even though the major structural oleosin proteins in seed LDs have been known for decades, the factors influencing LD formation and dynamics are still being uncovered mostly in the “model oilseed” Arabidopsis. Here we identified several key LD biogenesis proteins in the seeds of pennycress, a potential biofuel crop, that were correlated previously with seed oil content and characterized here for their participation in LD formation in transient expression assays and stable transgenics. One pennycress protein, the lipid droplet associated protein‐interacting protein (LDIP), was able to functionally complement the Arabidopsis ldip mutant, emphasizing the close conservation of lipid storage among these two Brassicas. Moreover, loss‐of‐function ldip mutants in pennycress exhibited increased seed oil content without compromising plant growth, raising the possibility that LDIP or other LD biogenesis factors may be suitable targets for improving yields in oilseed crops more broadly.

Guzha, Athanas↗
DOE OSTI2026

Effect of Oil Viscosity and Impact of Ionic Liquid Additive on Electrically Induced Pitting in Rolling Contact

Electrically induced bearing damage (EIBD) is a growing concern in electric drivetrains, where electrical discharge currents generate localized pitting that accelerates wear and premature failure. While ionic liquids (ILs) have shown promise as lubricant additives due to their natural physical adsorption, strong capabilities of tribofilm formation, and high molecular tunability, their role in mitigating EIBD is little known. This gap is especially evident because lubricant viscosity and film thickness affect discharge behavior, and additive performance in these regimes has not been well explored. In this study, tribological tests were performed using a ball-on-disk pure rolling-contact system under an applied voltage on polyalphaolefin oils of three viscosities (4, 10, and 150 cSt measured at 100 °C), with and without the addition of a phosphonium-phosphate IL. Surface damage was analyzed using scanning electron microscopy (SEM) and stylus profilometry. Damage quantification was based on pit morphology analysis of SEM images. The results provide new insights into how oil viscosity governs the discharge behavior and how an IL additive could influence the pit formation. While viscosity seems to play a significant role, an IL additive shows potential to reduce EIBD and deserves further study.

electrically induced bearing damage (EIBD)↗
DOE OSTI2013

Radiological issues associated with the recent boom in oil and gas hydraulic fracturing

As the worldwide hydraulic fracturing 'fracking' market continued to grow to an estimated $37 Billion in 2012, the need to understand and manage radiological issues associated with fracking is becoming imperative. Fracking is a technique that injects pressurized fluid into rock layer to propagate fractures that allows natural gas and other petroleum products to be more easily extracted. Radioactivity is associated with fracking in two ways. Radioactive tracers are frequently a component of the injection fluid used to determine the injection profile and locations of fractures. Second, because there are naturally-occurring radioactive materials (NORM) in the media surrounding and containing oil and gas deposits, the process of fracking can dislodge radioactive materials and transport them to the surface in the wastewater and gases. Treatment of the wastewater to remove heavy metals and other contaminates can concentrate the NORM into technologically-enhanced NORM (TENORM). Regulations to classify, transport, and dispose of the TENORM and other radioactive waste can be complicated and cumbersome and vary widely in the international community and even between states/provinces. In many cases, regulations on NORM and TENORM do not even exist. Public scrutiny and regulator pressure will only continue to increase as the world demands on oil and gas continue to rise and greater quantities of TENORM materials are produced. Industry experts, health physicists, regulators, and public communities must work together to understand and manage radiological issues to ensure reasonable and effective regulations protective of the public, environment, and worker safety and health are implemented. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗
DOE OSTI2015

Genomics and Transcriptomics Analyses of the Oil-Accumulating Basidiomycete Yeast Trichosporon oleaginosus : Insights into Substrate Utilization and Alternative Evolutionary Trajectories of Fungal Mating Systems

Microbial fermentation of agro-industrial waste holds great potential for reducing the environmental impact associated with the production of lipids for industrial purposes from plant biomass. However, the chemical complexity of many residues currently prevents efficient conversion into lipids, creating a high demand for strains with the ability to utilize all energy-rich components of agricultural residues. Here, we present results of genome and transcriptome analyses of Trichosporon oleaginosus. This oil-accumulating yeast is able to grow on a wide variety of substrates, including pentoses and N-acetylglucosamine, making it an interesting candidate for biotechnological applications. Transcriptomics shows specific changes in gene expression patterns under lipid-accumulating conditions. Furthermore, gene content and expression analyses indicate that T. oleaginosus is well-adapted for the utilization of chitin-rich biomass. We also focused on the T. oleaginosus mating type, because this species is a member of the Tremellomycetes, a group that has been intensively analyzed as a model for the evolution of sexual development, the best-studied member being Cryptococcus neoformans. The structure of the T. oleaginosus mating-type regions differs significantly from that of other Tremellomycetes and reveals a new evolutionary trajectory paradigm. Comparative analysis shows that recruitment of developmental genes to the ancestral tetrapolar mating-type loci occurred independently in the Trichosporon and Cryptococcus lineages, supporting the hypothesis of a trend toward larger mating-type regions in fungi. IMPORTANCE: Finite fossil fuel resources pose sustainability challenges to society and industry. Microbial oils are a sustainable feedstock for biofuel and chemical production that does not compete with food production. We describe genome and transcriptome analyses of the oleaginous yeast Trichosporon oleaginosus, which can accumulate up to 70% of its dry weight as lipids. In contrast to conventional yeasts, this organism not only shows an absence of diauxic effect while fermenting hexoses and pentoses but also effectively utilizes xylose and N-acetylglucosamine, which are building blocks of lignocellulose and chitin, respectively. Transcriptome analysis revealed metabolic networks that govern conversion of xylose or N-acetylglucosamine as well as lipid accumulation. These data form the basis for a targeted strain optimization strategy. Furthermore, analysis of the mating type of T. oleaginosus supports the hypothesis of a trend toward larger mating-type regions in fungi, similar to the evolution of sex chromosomes in animals and plants.

10 SYNTHETIC FUELS↗
DOE OSTI2025

Unraveling the ecological success of Iodidimonas in a bioreactor treating oil and gas produced water

Iodidimonas sp., a bacterium found in bioreactors treating oil and gas produced water as well as iodide-rich brines, has garnered attention for its unique ability to oxidize iodine. However, little is known about the metabolic capabilities that enable Iodidimonas sp. to thrive in certain unique ecological niches. In this study, we isolated, characterized, and sequenced three strains belonging to the Iodidimonas genus from the sludge of a membrane bioreactor used for produced water treatment. We investigated the genomic features of these isolates and compared them with the four publicly available isolate genomes from this genus, as well as a metagenome-assembled genome from the source bioreactor. Our Iodidimonas isolates had several genes associated with mitigating salinity, heavy metal, and organic compound stress, which likely help these bacteria to survive in produced water. Phenotyping tests revealed that while the isolates could utilize a wide variety of simple carbon substrates, they failed to degrade aliphatic or aromatic hydrocarbons, consistent with the lack of genes associated with common hydrocarbon degradation pathways in their genomes. We hypothesize that these microbes may lead a scavenging lifestyle in the bioreactor and similar iodide-rich brines. IMPORTANCE: Occupying a niche habitat and having few representative isolates, the genus Iodidimonas is a relatively understudied alphaproteobacterial group. Its ability to corrode pipes in iodine production facilities has economic implications, and its ability to generate potentially carcinogenic iodinated organic compounds during treatment of oil and gas produced water may cause environmental and health concerns with the recycling of treated water. Therefore, detailed characterization of the metabolic potential of the Iodidimonas isolates in this study both sheds light on their adaptation to the environmental conditions they inhabit and has environmental and economic significance.

Acharya, Shwetha M↗
DOE OSTI2017

Numerical simulation of diverse thermal in situ upgrading processes for the hydrocarbon production from kerogen in oil shale reservoirs

© 2017, © The Author(s) 2017. We investigate the productivity and product selectivity of diverse thermal in situ upgrading processes in oil shale reservoirs. In situ upgrading processes applying the ideas of Shell In situ Conversion Process, ExxonMobil Electrofrac, and Texas A&M Steamfrac are simulated by using sector models with the assumption of symmetric patterns. In-house fully functional simulator is used, which has been developed for the kerogen pyrolysis and hydrocarbon production. In the simulation cases, sensitivity analyses to the factors having major influence on the productivity and product selectivity are conducted. The effects of the temperature of vertical heaters, the spacing of hydraulic fractures, and the position of horizontal production wells are analyzed in the applied In situ Conversion Process, Electrofrac, and Steamfrac, respectively. In the applied In situ Conversion Process cases, hydrocarbon production increases with the increasing heater temperature. In the applied Electrofrac cases, hydrocarbon production increases with the increasing spacing of hydraulic fractures, even though longer time period for the process is needed. In the applied Steamfrac cases, the case of production well located at the same depth to the injection well shows the least hydrocarbon production. Among the processes, the applied In situ Conversion Process cases show the highest weight percentage of total hydrocarbon components in the produced fluid, and the applied Electrofrac cases follow it. The applied Steamfrac cases show far lower weight percentage of hydrocarbon production than the other methods. In terms of the mass ratio of produced hydrocarbon to decomposed kerogen, the applied Steamfrac cases show the largest value among the processes by aqueous phase sweeping liquid organic phase, but they also show the huge water oil mass ratio by the continuous injection of hot water. All the applied In situ Conversion Process cases and the Electrofrac case with the short spacing of hydraulic fractures show good heating efficiency by decomposing whole kerogen in the system.

Lee, Kyung Jae↗
DOE OSTI2018

Cost of Oil and Biomass Supply Shocks under Different Biofuel Supply Chain Configurations

This analysis estimates the cost of selected oil and biomass supply shocks for producers and consumers in the light-duty vehicle fuel market under various supply chain configurations using a mathematical programing model, BioTrans. The supply chain configurations differ by whether they include selected flexibility levers: multi-feedstock biorefineries; advanced biomass logistics; and the ability to adjust ethanol content of low-ethanol fuel blends, from E10 to E15 or E05. The simulated scenarios explore market responses to supply shocks including substitution between gasoline and ethanol, substitution between different sources of ethanol supply, biorefinery capacity additions or idling, and price adjustments. Welfare effects for the various market participants represented in BioTrans are summarized into a net shock cost measure. As oil accounts for a larger fraction of fuel by volume, its supply shocks are costlier than biomass supply shocks. Corn availability and the high cost of adding biorefinery capacity limit increases in ethanol use during gasoline price spikes. During shocks that imply sudden decreases in the price of gasoline, the renewable fuel standard (RFS) biofuel blending mandate limits the extent to which flexibility can be exercised to reduce ethanol use. The selected flexibility levers are most useful in response to cellulosic biomass supply shocks.

Uria Martinez, Rocio↗
DOE OSTI2019

Application of a convolutional neural network in permeability prediction: A case study in the Jacksonburg-Stringtown oil field, West Virginia, USA

Permeability is a critical parameter for understanding subsurface fluid flow behavior, managing reservoirs, enhancing hydrocarbon recovery, and sequestering carbon dioxide. In general, permeability is measured in the laboratory based on subsurface core samples, calculated from well logs or estimated from well tests. However, laboratory measurements and well tests are expensive, time-consuming, and usually limited to a few core samples or wells in a hydrocarbon field or carbon storage site. Machine-learning techniques are good options for generating a rapid, robust, and cost-effective permeability prediction model because of their strengths to recognize the potential interrelationships between input and output variables. Convolutional neural networks (CNN), as a good pattern recognition algorithm, are widely used in image processing, natural language processing, and speech recognition, but are rarely used with regression problems and even less often in reservoir characterization. We have developed a CNN regression model to estimate the permeability in the Jacksonburg-Stringtown oil field, West Virginia, which is a potential carbon storage site and enhanced oil recovery operations field. We also evaluate the concept of the geologic feature image, which is converted from geophysical well logs. Five variables, including two commonly available conventional well logs (the gamma rays [GRs] and bulk density) and three well-log-derived variables (the slopes of the GR and bulk density curves, and shale content), are used to generate a geologic feature image. The CNN treats the geologic feature image as the input and the permeability as the desired output. In addition, the permeability predicted using traditional backpropagation artificial neural networks, which are optimized by genetic algorithms and particle swarm optimization, is compared with the permeability estimated using our CNN. Our results indicate that the CNN regression model provides more accurate permeability predictions than the traditional neural network.

Geochemistry & Geophysics↗
DOE OSTI2023

Procedure for locating oil and gas wells in the Appalachian Basin

Locating undocumented (or poorly documented) oil and gas wells for environmental assessment is often difficult. Remnant features that confirm the presence of a well (intact casing/wellhead, well bore, etc.) are typically less than a meter in size and often are obscured from direct observation on the ground or from the air (by dense vegetation, for example). To efficiently find such features, it is useful to first systematically compile publicly available digital data at progressively smaller scales prior to embarking on field campaigns. Further, the information presented here describes the procedure developed and used by the U.S. Department of Energy's National Energy Technology Laboratory to locate potential oil and gas well sites for follow-up field verification and characterization. Digital data are first compiled from national and state resources such as well location/production databases, historical topographic maps, historical aerial photographs, and LiDAR data. Although each data set is likely to be incomplete or inaccurate to some extent, combining the data resources using geographic information system technology can generate potential well site targets with a higher degree of confidence, which improves the efficiency of fieldwork activities. This workflow was developed in the Appalachian Basin region, and although certain aspects may be unique, the general process would be applicable to locating undocumented wells in other regions.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2018

Development of an Open Global Oil and Gas Infrastructure Inventory and Geodatabase

This submission contains a technical report describing the development process and visual graphics for the Global Oil and Gas Infrastructure database. Access the GOGI database using the following link: https://edx.netl.doe.gov/dataset/global-oil-gas-features-database There is a web-mapping tool that allows you to visualize and interact with this data also available here: https://edxspatial.arcgis.netl.doe.gov/maps/edxspatial-gogi-index.html Acknowledgement: This work was performed under a CRADA between NETL and EDF, and was funded under the Climate and Clean Air Coalition (CCAC) Oil and Gas Methane Science Studies. The studies are managed by United Nations Environment in collaboration with the Office of the Chief Scientist, Steven Hamburg of the Environmental Defense Fund. Funding was provided by the Environmental Defense Fund, OGCI Companies (Shell, BP, ENI, Petrobras, Repsol, Total, Equinor, CNPC, Saudi Aramco, Exxon, Oxy, Chevron, Pemex) and CCAC.

GOGI↗
DOE OSTI2013

Feasibility Studies on Process Coupling of Transesterification and Methanol Synthesis Using Cellulose and Bio-oil

The Sponsored Programs of WKU is the compliance management for the entire campus. They are responsible for both the fiscal compliance, in addition to the coordination and implementation of pre and post-award documentation. Dr. Wei-Ping Pan is the Principal Investigator of the project, and is responsible for the carrying out of the objectives outlined in this proposal. Dr. Yan Cao, serve as the Co-Principal Investigator, who is responsible for directing the team in carrying out the objectives outlined. This project investigates feasibilities of combining the transesterification bio-diesel production into the traditional methanol production process. In this process, the raw materials for process feedstock will be completely the biomass, include cellulose fractions of biomass and bio-oil fractions of biomass. The process product will be either the bio-diesel or mixed bio-diesel and bio-methanol, which both can be directly fed as the alterative liquid fuel and also has some improvement on fuel handling. Since its capability of the complete utilization of every part of biomass (both cellulose biomass and bio-oils) and capability of the in-site optimization of reaction heat transfer and thermodynamics equilibrium shift and instant separation of process product, the proposed process can better balance several drawbacks of individual process during production of bio-diesel. This is a totally “green” process with almost all aspects of process to embody the characterization of renewable, compatibility and economic.

10 SYNTHETIC FUELS↗