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At least 1,081 records · Page 60

Axenisation of oleaginous microalgal cultures via anoxic photosensitisation

Growing interest in sustainable food and biofuel research has necessitated high quality axenic oleaginous microalgal strains. Unfortunately, most strains available in culture banks contain commensal microbes such as bacteria and the default decontamination method involves antibiotic treatment which has begun to exacerbate the emergence of antibiotic resistance. To overcome this problem, anoxic photosensitisation was investigated as an alternate approach. Four oleaginous microalgal species (Tetradesmus obliquus, Desmodesmus armatus, Chlorella vulgaris and Nannochloropsis limnetica) were incubated in varying concentrations of Rose Bengal (0 μM, 1 μM, 3 μM or 9 μM) either in normal (oxic) or anoxic conditions, for 72 h under light (8.85 ± 0.4 W/m 2 ) in a specially designed heterotrophic growth complex (HGC) medium, followed by 72 h in standard Bold's Basal Medium (BBM). Commonly used antibiotics-based protocol was used as the control method. Post treatment, cell numbers and percentage populations were counted with Flow Cytometry, and viability was tested using standard plating methods using BBM and LB. Additionally, the contaminating microbes in the cultures were profiled using 16Ss rRNA sequencing. Anoxic conditions were able to significantly decrease bacterial content, albeit with an equally detrimental effect on the microalgal population. Although the responses differed between the microalgae, anoxic incubation along with Rose Bengal at 3 μM was able to completely decontaminate N. limnetica and C. vulgaris, while D. armatus and T. obliquus could be decontaminated with an additional streak-plating step. None of the cultures could be decontaminated using antibiotics treatment. These results suggest that axenisation of microalgal cultures was largely due to anoxy, that was synergistically enhanced by Rose Bengal at a concentration of ≥3 μM.

59 BASIC BIOLOGICAL SCIENCES↗

Energy efficiency and biological interactions define the core microbiome of deep oligotrophic groundwater

While oligotrophic deep groundwaters host active microbes attuned to the low-end of the bioenergetics spectrum, the ecological constraints on microbial niches in these ecosystems and their consequences for microbiome convergence are unknown. Here, we provide a genome-resolved, integrated omics analysis comparing archaeal and bacterial communities in disconnected fracture fluids of the Fennoscandian Shield in Europe. Leveraging a dataset that combines metagenomes, single cell genomes, and metatranscriptomes, we show that groundwaters flowing in similar lithologies offer fixed niches that are occupied by a common core microbiome. Functional expression analysis highlights that these deep groundwater ecosystems foster diverse, yet cooperative communities adapted to this setting. We suggest that these communities stimulate cooperation by expression of functions related to ecological traits, such as aggregate or biofilm formation, while alleviating the burden on microorganisms producing compounds or functions that provide a collective benefit by facilitating reciprocal promiscuous metabolic partnerships with other members of the community. We hypothesize that an episodic lifestyle enabled by reversible bacteriostatic functions ensures the subsistence of the oligotrophic deep groundwater microbiome.

54 ENVIRONMENTAL SCIENCES↗

Collective behavior of “flexicles”

In recent years the functionality of synthetic active microparticles has edged even closer to that of their biological counterparts. However, we still lack the understanding needed to recreate at the microscale key features of autonomous behavior exhibited by microorganisms or swarms of macroscopic robots. In this study, we propose a model for a three-dimensional deformable cellular composite particle consisting of self-propelled rod-shaped colloids confined within a flexible vesicle—representing a superstructure we call a “flexicle” that couples particle deformation to the internal dynamics of the internal active components. Using molecular dynamics simulations, we investigate the collective behavior of dense systems composed of many flexicles. We show that individual flexicles exhibit shape changes upon collisions with other flexicles that lead to rearrangements of the internal active rods, which slows flexicle motion. This shape deformability gives rise to a diverse set of motility-induced phase separation phenomena and the spontaneous flow of flexicles reminiscent of the migration of cells in dense tissues. Our findings establish a foundation for designing responsive, cell-like active particles and developing strategies for controlling swarm migration and other autonomous swarm behaviors at cellular and colloidal scales.

Science & Technology - Other Topics↗

The differential rotation of the solar surface

The large-scale flow in the solar convection zone is discussed. The objective is to deduce from observation the principal physical balances in the governing equations. The simplest set of equations that seem potentially realistic are utilized. It is assumed that magnetic fields are negligible, that mixing-length theory gives an accurate representation of the mean structure, and that rigid-body rotation exists at the base. It is deduced that the zonal glow is geostrophic, the meridional flow is controlled by friction, and diffusive heating balances advective cooling due to vertical motion. Next, a detailed calculation of the latitude profile of surface angular velocity is performed, based on approximate equations containing only the principal balances, and agrees well with observation. Finally, it is demonstrated that the amplitude of the flow may be determined by the constraint that the net equatorward angular momentum flux vanish. The conclusions are consistent with the hypothesis that the flow is primarily a single large axisymmetric convection cell in each hemisphere.

Gierasch, P. J.↗

Automated Array Assembly, Phase 2. Low-cost Solar Array Project, Task 4

Work was done to verify the technological readiness of a select process sequence with respect to satisfying the Low Cost Solar Array Project objectives of meeting the designated goals of $.50 per peak watt in 1986 (1975 dollars). The sequence examined consisted of: (1) 3 inches diameter as-sawn Czochralski grown 1:0:0 silicon, (2) texture etching, (3) ion implanting, (4) laser annealing, (5) screen printing of ohmic contacts and (6) sprayed anti-reflective coatings. High volume production projections were made on the selected process sequence. Automated processing and movement of hardware at high rates were conceptualized to satisfy the PROJECT's 500 MW/yr capability. A production plan was formulated with flow diagrams integrating the various processes in the cell fabrication sequence.

Lopez, M.↗

Advanced screening of electrode couples

The chromium (Cr(3+)/Cr(2+)) redox couple (electrolyte and electrode) was investigated to determine its suitability as negative electrode for the iron (Fe(3+)/Fe(2+))-chromium (Cr(3+)/Cr(2+)) redox flow battery. Literature search and laboratory investigation established that the solubility and stability of aqueous acidic solutions of chromium(3) chloride and chromium(2) chloride are sufficient for redox battery application. Four categories of electrode materials were tested; namely, metals and metalloid materials (elements and compounds), alloys, plated materials, and Teflon-bonded materials. In all, the relative performance of 26 candidate electrode materials was evaluated on the basis of slow scan rate linear sweep voltammetry in stirred solution. No single material tested gave both acceptable anodic an acceptable cathodic performance. However, the identification of lead as a good cathodic electrocatalyst and gold as a good anodic electrocatalyst led to the invention of the lead/gold combination electrocatalyst. This type of catalyst can be fabricated in several ways and appears to offer the advantages of each metal without the disadvantages associated with their use as single materials. This lead/gold electrocatalyst was tested by NASA-Lewis Research Center in complete, flowing, redox batteries comprising a stack of several cells. A large improvement in the battery's coulombic and energy efficiency was observed.

Giner, J. D.↗

Stellar convection theory. III - Dynamical coupling of the two convection zones in A-type stars by penetrative motions

The thermal convection occurring over many density scale heights in an A-type star outer envelope, encompassing both the hydrogen and helium convectively unstable zones, is examined by means of anelastic modal equations. The single-mode anelastic equations for such compressible convection display strong overshooting of the motions into adjacent radiative zones, which would preclude diffusive separation of elements in the supposedly quiescent region between the two unstable zones. In addition, the anelastic solutions reveal that the two zones of convective instability are dynamically coupled by the overshooting motions. The two solutions that the nonlinear single-mode equations admit for the same horizontal wavelength are distinguished by the sense of the vertical velocity at the center of the three-dimensional cell. It is suggested that strong horizontal shear flows should be present just below the surface of the star, and that the large-scale motions extending into the stable atmosphere would appear mainly as horizontal flows.

Latour, J.↗

Detection of rotating thunderstorms using satellite imagery

In the case of the Carolina tornadoes, researchers prepared visible and IR GOES imagery covering the period 2000 Z when the storm entered South Carolina from Georgia until it exited North Carolina at 0200 Z into Virginia. The GOES IR imagery clearly demonstrated that this storm was imbedded in a continuously propagating mesolow with a well defined cold dome. The ground damage track paralleled exactly with the cold dome throughout the storm's life across the Carolinas. There were no advanced very high resolution radiometer (AVHRR) data during the period to allow researchers to inspect the cloud top for warm temperature anomalies. The Carolina storm did exhibit rightward deviating outflow which was oriented about 60 degrees to the 300 mb streamlines. The tornadoes of April 27, 1984 were part of a tornado producing cold front which stretched from Oklahoma to Minnesota. As the front moved eastward it touched off numerous tornadoes in eastern Wisconsin. GOES imagery for this data was prepared and it was strikingly clear that all along the North-South oriented squall line, the individual tunderstorms had cirrus plumes which had remarkable right deviation to the upper air flow. Unlike the Carolina long track supercell cell-mesolow system, these storms were isolated individual thunderstorms which touched off at least 16 tornadoes in eastern Wisconsin stretching from the Milwaukee area on the south to Vilas County in the north. The monster tornado of June 8, 1984 which leveled 90 percent of the village of Barneveld, Wisconsin and killed 9 persons is also discussed.

Anderson, C. E.↗

Passivation of Si solar cells by hetero-epitaxial compound semiconductor coatings

A development status evaluation is made for high efficiency Si solar cells, with emphasis on the suppression of the deleterious effects of surface recombination. ZnS(0.9)Se(0.1) and GaP are identified as candidates for the reduction of surface recombination. Attention is given to methods developed for the deposition of heteroepitaxial compounds designed to block minority carrier transport to the Si solar cell surface without interfering with the majority carrier flow.

Vernon, S. M.↗

On the Thermal Model of Transverse Flow of Unidirectional Materials

The thermal model for transverse heat flow of having single filament in a unit cell is extended. In this model, we proposed that two circular filaments in a unit cell of square packing array and obtained the transverse thermal conductivity of an unidirectional material.

Tai, Hsiang↗

Hydrodynamic Modeling of Oxidizer-Rich Staged Combustion Injector Flow

The main objective of this work is to determine the unsteady hydrodynamic characteristics of coaxial swirl atomizers of interest in oxidizer-rich staged combustion (ORSC) liquid rocket engines. To this end, the pseudo-density (homogeneous flow) treatment combined with the Marker-and-Cell (MAC) numerical algorithm has been used to develop an axisymmetric with swirl, two-phase, unsteady model. The numerical model is capable of assessing the time-dependent orifice exit conditions and internal mixing for arbitrary fuel and oxidizer gas injection conditions. Parametric studies have been conducted to determine the effect of geometry, gas properties, and liquid properties on the exit massflow rate and velocity. It has been found that the frequency at which the liquid film oscillates increases as the density ratio and thickness increase, decreases as film thickness and liquid swirl velocity increase, and is unaffected by the mixing length. Additionally, it has been determined that the variation in the massflow rate increases as the liquid swirl velocity and liquid film thickness increase, and decreases as the density ratio, collar thickness, and mixing length increase.

Canino, J. V.↗

Numerical, Analytical, Experimental Study of Fluid Dynamic Forces in Seals: Description of Gas Seal Codes GCYLT and GFACE - Volume 2

The objectives of the program were to develop computational fluid dynamics (CFD) codes and simpler industrial codes for analyzing and designing advanced seals for air-breathing and space propulsion engines. The CFD code SCISEAL is capable of producing full three-dimensional flow field information for a variety of cylindrical configurations. An implicit multidomain capability allow the division of complex flow domains to allow optimum use of computational cells. SCISEAL also has the unique capability to produce cross-coupled stiffness and damping coefficients for rotordynamic computations. The industrial codes consist of a series of separate stand-alone modules designed for expeditious parametric analyses and optimization of a wide variety of cylindrical and face seals. Coupled through a Knowledge-Based System (KBS) that provides a user-friendly Graphical User Interface (GUI), the industrial codes are PC based using an OS/2 operating system. These codes were designed to treat film seals where a clearance exists between the rotating and stationary components. Leakage is inhibited by surface roughness, small but stiff clearance films, and viscous pumping devices. The codes have demonstrated to be a valuable resource for seal development of future air-breathing and space propulsion engines.

Liang, Anita D.↗

Numerical, Analytical, Experimental Study of Fluid Dynamic Forces in Seals: Executive Summary and Description of Knowledge-Based System - Volume 1

The objectives of the program were to develop computational fluid dynamics (CFD) codes and simpler industrial codes for analyzing and designing advanced seals for air-breathing and space propulsion engines. The CFD code SCISEAL is capable of producing full three-dimensional flow field information for a variety of cylindrical configurations. An implicit multidomain capability allows the division of complex flow domains to allow optimum use of computational cells. SCISEAL also has the unique capability to produce cross-coupled stiffness and damping coefficients for rotordynamic computations. The industrial codes consist of a series of separate stand-alone modules designed for expeditious parametric analyses and optimization of a wide variety of cylindrical and face seals. Coupled through a Knowledge-Based System (KBS) that provides a user-friendly Graphical User Interface (GUI), the industrial codes are PC based using an OS/2 operating system. These codes were designed to treat film seals where a clearance exists between the rotating and stationary components. Leakage is inhibited by surface roughness, small but stiff clearance films, and viscous pumping devices. The codes have demonstrated to be a valuable resource for seal development of future air-breathing and space propulsion engines.

Liang, Anita D.↗

Numerical, Analytical, Experimental Study of Fluid Dynamic Forces in Seals: Description of Spiral-Groove Codes SPIRALG and SPIRALI - Volume 3

The objectives of the program were to develop computational fluid dynamics (CFD) codes and simpler industrial codes for analyzing and designing advanced seals for air-breathing and space propulsion engines. The CFD code SCISEAL is capable of producing full three-dimensional flow field information for a variety of cylindrical configurations. An implicit multidomain capability allow the division of complex flow domains to allow optimum use of computational cells. SCISEAL also has the unique capability to produce cross-coupled stiffness and damping coefficients for rotordynamic computations. The industrial codes consist of a series of separate stand-alone modules designed for expeditious parametric analyses and optimization of a wide variety of cylindrical and face seals. Coupled through a Knowledge-Based System (KBS) that provides a user-friendly Graphical User Interface (GUI), the industrial codes are PC based using an OS/2 operating system. These codes were designed to treat film seals where a clearance exists between the rotating and stationary components. Leakage is inhibited by surface roughness, small but stiff clearance films, and viscous pumping devices. The codes have demonstrated to be a valuable resource for seal development of future air-breathing and space propulsion engines.

Liang, Anita D.↗

Numerical, Analytical, Experimental Study of Fluid Dynamic Forces in Seals: Description of Incompressible Fluid Seal Codes ICYL and IFACE - Volume 4

The objectives of the program were to develop computational fluid dynamics (CFD) codes and simpler industrial codes for analyzing and designing advanced seals for air-breathing and space propulsion engines. The CFD code SCISEAL is capable of producing full three-dimensional flow field information for a variety of cylindrical configurations. An implicit multidomain capability allow the division of complex flow domains to allow optimum use of computational cells. SCISEAL also has the unique capability to produce cross-coupled stiffness and damping coefficients for rotordynamic computations. The industrial codes consist of a series of separate stand-alone modules designed for expeditious parametric analyses and optimization of a wide variety of cylindrical and face seals. Coupled through a Knowledge-Based System (KBS) that provides a user-friendly Graphical User Interface (GUI), the industrial codes are PC based using an OS/2 operating system. These codes were designed to treat film seals where a clearance exists between the rotating and stationary components. Leakage is inhibited by surface roughness, small but stiff clearance films, and viscous pumping devices. The codes have demonstrated to be a valuable resource for seal development of future air-breathing and space propulsion engines.

Liang, Anita D.↗

Numerical, Analytical, Experimental Study of Fluid Dynamic Forces in Seals Volume 6: Description of Scientific CFD Code SCISEAL

The objectives of the program were to develop computational fluid dynamics (CFD) codes and simpler industrial codes for analyzing and designing advanced seals for air-breathing and space propulsion engines. The CFD code SCISEAL is capable of producing full three-dimensional flow field information for a variety of cylindrical configurations. An implicit multidomain capability allow the division of complex flow domains to allow optimum use of computational cells. SCISEAL also has the unique capability to produce cross-coupled stiffness and damping coefficients for rotordynamic computations. The industrial codes consist of a series of separate stand-alone modules designed for expeditious parametric analyses and optimization of a wide variety of cylindrical and face seals. Coupled through a Knowledge-Based System (KBS) that provides a user-friendly Graphical User Interface (GUI), the industrial codes are PC based using an OS/2 operating system. These codes were designed to treat film seals where a clearance exists between the rotating and stationary components. Leakage is inhibited by surface roughness, small but stiff clearance films, and viscous pumping devices. The codes have demonstrated to be a valuable resource for seal development of future air-breathing and space propulsion engines.

Athavale, Mahesh↗

Numerical, Analytical, Experimental Study of Fluid Dynamic Forces in Seals: Description of Seal Dynamics Code DYSEAL and Labyrinth Seals Code KTK - Volume 5

The objectives of the program were to develop computational fluid dynamics (CFD) codes and simpler industrial codes for analyzing and designing advanced seals for air-breathing and space propulsion engines. The CFD code SCISEAL is capable of producing full three-dimensional flow field information for a variety of cylindrical configurations. An implicit multidomain capability allow the division of complex flow domains to allow optimum use of computational cells. SCISEAL also has the unique capability to produce cross-coupled stiffness and damping coefficients for rotordynamic computations. The industrial codes consist of a series of separate stand-alone modules designed for expeditious parametric analyses and optimization of a wide variety of cylindrical and face seals. Coupled through a Knowledge-Based System (KBS) that provides a user-friendly Graphical User Interface (GUI), the industrial codes are PC based using an OS/2 operating system. These codes were designed to treat film seals where a clearance exists between the rotating and stationary components. Leakage is inhibited by surface roughness, small but stiff clearance films, and viscous pumping devices. The codes have demonstrated to be a valuable resource for seal development of future air-breathing and space propulsion engines

Shapiro, Wilbur↗