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NASA NTRSDate not supplied

Developing a Genetic Variant Calling Pipeline for Quantifying the Complex Mutagenic Load Accumulated in BioNutrients-1 Production Pack Samples

Microorganisms hold great promise for on demand production of labile nutrients and pharmaceuticals as well recycling and in situ resource utilization. The utilization of microorganisms for such tasks on space missions is hindered by the limited data on how microbes respond to spaceflight. For example, the genetic stability of microorganisms, and the genomic engineered traits added to deliver desired functions, over long-term storage in the spacecraft environment is poorly understood. The BioNutrients-1 (BN-1) mission conducted a 5-year study of desiccated storage in Low Earth Orbit (LEO) to evaluate the suitability of eight synthetic biology chassis organisms for long-duration space missions. We are employing high-depth, whole genome sequencing (WGS) to determine the mutagenic load that accumulated during long-term storage. Mutation analysis pipelines are well established for homogenous culture grown from a single colony, but the mutational landscape of the BN-1 samples present a unique analysis challenge, as every cell in the BN-1 samples had a unique genetic journey of DNA damage and repair. Consequently, sequence variants are expected at low allele frequency within samples. To address this genetic complexity, we apply two distinct computational approaches to identify mutations in pre-existing WGS data collected from populations of Chlamydomonas reinhardtii that were exposed to UV mutagenesis and growth in LEO. For reference genome free mutation detection, we utilized DiscoSNP++, which is a de Bruijn graph approach. For reference genome-based mutation detection we utilize GATK for Microbes, which is a Bayesian probabilistic approach. We will benchmark these approaches against the mutations originally identified using CRISP, a method optimized for pooled samples. Ultimately, quantifying the mutation load imposed by storage or growth on the ISS will help identify chassis organisms with both high levels of genome stability and viability, which are desirable traits for implementation of bioproduction in long-duration missions.

SNP↗

NASA NTRSDate not supplied

Genesis Solar Wind – Capture, Return, Curate and Analyze: Looking Backward Reviewing Sample Collection Hardware

Genesis, as the first U.S. spacecraft to return astromaterial samples since Apollo, not only integrated the mission planning and flight teams, but also the science and sample curation teams during the mission development period. Since Genesis is a sample return mission, the Science Team was essential in certifying the collectors (sample containers for solar atoms). Burnett and the science team defined the purity of collector materials and ability to analyze solar wind composition to the precision required for planetary science. The science team’s choices included a variety of very pure materials which are described, including rationale for selection. The JPL Project Scientist networked with specialty vendors refining specifications and fabricating several of the materials. JPL engineering team designed and fabricated the collector arrays. LANL designed and built an electrostatic concentrator. JSC procured these materials with exacting specifications.

SOLAR WIND↗

NASA NTRS1999

Mars Sample Return Navigation: Rendezvous in Mars Orbit

A mission to retrieve Martian rock and soil samples is under development by an international Mars Sample Return (MSR) design team managed by JPL. The first Martian samples are collected and launched into low Mars orbit in 2004.

Mars sample return Navigation Mars Orbit soil samp↗

NASA NTRS2002

Mars sample return, updated to a groundbreaking approach

A Mars Sample Return (MSR) mission is a goal of the Mars Program. Recently, NASA and JPL have been studying the possibility of a Mars Sample Return some time in the next decade of Mars exploration. In 2001, JPL commissioned four industry teams to make a fresh examination of MSR architectures. Six papers on these studies were presented at last year's conference. As new fiscal realities of a cost-capped Mars Exploration Program unfolded, it was evident that these MSR concepts, which included mobility and subsurface sample acquisition, did not fit reasonably within a balanced program. Therefore, at the request of NASA and the science community, JPL asked the four industry teams plus JPL's Team X to explore ways to reduce the cost of a MSR. A NASA-created MSR Science Steering Group (SSG) established a reduced set of requirements for these new studies that built upon the previous year's work. As a result, a new 'Groundbreaking' approach to MSR was established that is well understood based on the studies and independent cost assessments by Aerospace Corporation and SAIC. The Groundbreaking approach appears to be what a contemporary, balanced Mars Exploration Program can afford, has turned out to be justifiable by the MSR Science Steering Group, and has been endorsed by the Mars science community at large. This paper gives a brief overview of the original 2001 study results and discusses the process leading to the new studies, the studies themselves, and the results.

Mars sample return↗

NASA NTRS2009

Transport Powder and Liquid Samples by Surface Acoustic Waves

Sample transport is an important requirement for In-situ analysis of samples in NASA planetary exploration missions. Tests have shown that powders or liquid drops on a surface can be transported by surface acoustic waves (SAW) that are generated on the surface using interdigital transducers. The phenomena were investigated experimentally and to generate SAWs interdigital electrodes were deposited on wafers of 128 deg rotated Y-cut LiNbO?. Transporting capability of the SAW device was tested using particles of various sizes and drops of various viscosities liquids. Because of different interaction mechanisms with the SAWs, the powders and the liquid drops were observed to move in opposite directions. In the preliminary tests, a speed of 180 mm/s was achieved for powder transportation. The detailed experimental setup and results are presented in this paper. The transporting mechanism can potentially be applied to miniaturize sample analysis system or " lab-on-chip" devices.

Sample handling↗

NASA NTRS2014

The Search for Organic Compounds of Martian Origin in Gale Crater by the Sample Analysis at Mars (SAM) Instrument on Curiosity

One of the key objectives of the Mars Science Laboratory rover and the Sample Analysis at Mars (SAM) instrument suite is to determine the inventory of organic and inorganic volatiles in the atmosphere and surface regolith and rocks to help assess the habitability potential of Gale Crater. The SAM instrument on the Curiosity rover can detect volatile organic compounds thermally evolved from solid samples using a combination of evolved gas analysis (EGA) and gas chromatography mass spectrometry (GCMS) (Mahaffy et al. 2012). The first solid samples analyzed by SAM, a scoop of windblown dust and sand at Rocknest, revealed several chloromethanes and a C4-chlorinated hydrocarbon derived primarily from reactions between a martian oxychlorine phase (e.g. perchlorate) and terrestrial carbon from N-methyl-N-(tertbutyldimethylsilyl)- trifluoroacetamide (MTBSTFA) vapor present in the SAM instrument background (Glavin et al. 2013). After the analyses at Rocknest, Curiosity traveled to Yellowknife Bay and drilled two separate holes in a fluvio-lacustrine sediment (the Sheepbed unit) designated John Klein and Cumberland. Analyses of the drilled materials by both SAM and the CheMin X-Ray Diffraction instrument revealed a mudstone consisting of ~20 wt% smectite clays (Ming et al. 2013; Vaniman et al. 2013), which on Earth are known to aid the concentration and preservation of organic matter. Oxychlorine compounds were also detected in the Sheepbed mudstone during pyrolysis; however, in contrast to Rocknest, much higher levels of chloromethanes were released from the Sheepbed materials, suggesting an additional, possibly martian source of organic carbon (Ming et al. 2013). In addition, elevated abundances of chlorobenzene and a more diverse suite of chlorinated alkanes including dichloropropane and dichlorobutane detected in Cumberland compared to Rocknest suggest that martian or meteoritic organic carbon sources may be preserved in the mudstone (Freissinet et al. 2013). Chloromethane and dichloromethane were also identified after thermal volatilization of the surface soils by the GCMS instruments at the Viking landing sites, although no other chlorinated hydrocarbons were reported (Biemann et al. 1977). Here we focus on the origin of the chlorinated hydrocarbons detected in the Sheepbed mudstone by SAM and the implications for the preservation of organic matter in near-surface materials on Mars.

Mars Curiosity Rover↗

NASA NTRS2014

Miniature Cryogenic Valves for a Titan Lake Sampling System

The Cassini mission has revealed Titan to be one of the most Earthlike worlds in the Solar System complete with many of the same surface features including lakes, river channels, basins, and dunes. But unlike Earth, the materials and fluids on Titan are composed of cryogenic organic compounds with lakes of liquid methane and ethane. One of the potential mission concepts to explore Titan is to land a floating platform on one of the Titan Lakes and determine the local lake chemistry. In order to accomplish this within the expected mass volume and power budgets there is a need to pursue the development for a low power lightweight cryogenic valves which can be used along with vacuum lines to sample lake liquid and to distribute to various instruments aboard the Lander. To meet this need we have initiated the development of low power cryogenic valves and actuators based on a single crystal piezoelectric flextensional stacks produced by TRS Ceramics Inc. Since the origin of such high electromechanical properties of Relaxor-PT single crystals is due to the polarization rotation effect, (i.e., intrinsic contributions), the strain per volt decrease at cryogenic temperatures is much lower than in standard Lead Zirconate Titanate (PZT) ceramics. This makes them promising candidates for cryogenic actuators with regards to the stroke for a given voltage. This paper will present our Titan Lake Sampling and Sample Handling system design and the development of small cryogenic piezoelectric valves developed to meet the system specifications.

Piezoelectric Devices↗

NASA NTRS2016

Synchronized Lunar Pole Impact Plume Sample Return Trajectory Design

The presented trajectory design enables two maneuverable spacecraft launched onto the same trans-lunar injection trajectory to coordinate a steep impact of a lunar pole and subsequent sample return of the ejecta plume to Earth. To demonstrate this concept, the impactor is assumed to use the LCROSS missions trajectory and spacecraft architecture, thus the permanently-shadowed Cabeus crater on the lunar south pole is assumed as the impact site. The sample-return spacecraft is assumed to be a CubeSat that requires a complimentary trajectory design that avoids lunar impact after passing through the ejecta plume to enable sample-return to Earth via atmospheric entry.

impact plume↗

NASA NTRS2017

Designing to Sample the Unknown: Lessons from OSIRIS-REx Project Systems Engineering

On September 8, 2016, the third NASA New Frontiers mission launched on an Atlas V 411. The Origins, Spectral Interpretation, Resource Identification, Security-Regolith Explorer (OSIRIS-REx) will rendezvous with asteroid Bennu in 2018, collect a sample in 2020, and return that sample to Earth in September 2023. The development team has overcome a number of challenges in order to design and build a system that will make contact with an unexplored, airless, low-gravity body. This paper will provide an overview of the mission, then focus in on the system-level challenges and some of the key system-level processes. Some of the lessons here are unique to the type of mission, like discussion of operating at a largely-unknown, low-gravity object. Other lessons, particularly from the build phase, have broad implications. The OSIRIS-REx risk management process was particularly effective in achieving an on-time and under-budget development effort. The systematic requirements management and verification and the system validation also helped identify numerous potential problems. The final assessment of the OSIRIS-REx performance will need to wait until the sample is returned in 2023, but this post-launch assessment will capture some of the key systems-engineering lessons from the development team.

Asteroid↗

NASA NTRS2020

Thermal Protection System Materials for Sample Return Missions

This white paper from the Thermal Protection System (TPS) community to the NRC Decadal Survey Sub-Panels provides an overview of TPS materials needed for future Sample Return (SR) missions. We consider the capability of heritage TPS material used by recent SR missions and identify appropriate materials for future SR missions. A prime conclusion is that the current TPS materials, if properly maintained, offer good low-density solutions for lower velocity (<13.5 km/s) sample return missions without planetary protection back-protection concerns. Furthermore, missions that have a larger capsule, a higher entry speed (>13.5 km/s), or back-protection concerns will leverage recently developed mid-density TPS materials. To maintain NASA’s Sample Return capabilities in the coming decade, we recommend that NASA continue to invest in sustainment of relevant TPS materials, as well as ground-test facilities, predictive entry modeling, and flight instrumentation.

mission design↗

NASA NTRSDate not supplied

Developing Geology Sampling Tools for the Artemis Program

Humans are set to return to the Moon for the first time since 1972 with the National Aeronautics and Space Administration’s (NASA) Artemis Program. One of the primary objectives will be the collection and return of lunar samples. To support this objective, the Extravehicular Activity (EVA) Tools Team at the Johnson Space Center (JSC) has started developing the next generation of lunar geology sampling tools. The EVA Tools Team are experts in the hardware certification process for space hardware and have been working on planetary surface tools for nearly a decade. Funded by the EVA Office at JSC, the Artemis tools project began on October 1, 2019 with an initial set of eight tools. That initial set of tools was brought to a Preliminary Design Review (PDR) on October 30, 2020. An additional 17tools have been proposed to support geology sampling operations, with the development of a subset of that list beginning on October 1, 2020.Additional tools may also be considered in the future should the science requirements drive this need. This abstract describes how this project is defining requirements, what tools are being developed, and the schedule for this work.

Artemis↗

NASA NTRS2023

Mars Sample Return and The Capture, Containment, and Return System New Design and Path To 2027 Launch

The Mars Sample Return (MSR) campaign is one of the most ambitious and complex exoplanet scientific exploration missions currently underway. With the participation of NASA, ESA, and a large number of industry partners, MSR aims to bring back Martian rock and atmosphere samples with the goal of answering key questions about Mars’ geological, climatological, and (potentially) biological evolution. To accomplish this ambitious goal, the campaign relies on three distinct flight elements and a ground element. The Earth Return Orbiter (ERO) mission that would host the Capture, Containment, and Return System (CCRS) is the last element of the trio. The mission would capture the orbiting sample in low Mars orbit (launched into orbit by another mission), contain it and return it to Earth, landing it at the Utah Test and Training Range (UTTR). The CCRS continues its development. Since its early architecture, several changes were adopted by the project in order to improve overall payload efficiency and reduce mass. CCRS is currently on track to achieve the next milestone, Critical Design Review, expected in late 2024. This paper will discuss the CCRS design, how the current architecture contributes to an improved mission baseline, and the next critical steps of the mission towards its launch.

Mars mission↗

NASA NTRSDate not supplied

Ultrapure Water System at JSC for High Purity Cleaning of Flight Hardware, Astromaterial Handling Tools and Solar Wind Samples

Use of Ultrapure Water (UPW) for cleaning of astromaterial sample handling tools and containers was driven by the discontinuation of Freon 113 as a cleaning solvent, due to environmental concerns. Freon 113, recycled using distillation, was very effective at removing organic residues from sample handling hardware. The initial switch to high purity water for astromaterial tool cleaning was modeled after efforts at White Sands Test Facility (WSTF) and current semiconductor UPW production practices in the early 1990s. JSC curation’s first system was comprised of ion exchange polishing cylinders, 55 gallon PVDF-lined storage tank, a few tens of feet of PVDF piping, UV and particle filtration, and this system supported cleaning for lunar, meteorite and Cosmic Dust collectors lab operations. By 2000 a major expansion and upgrade was providing UPW of quality E-1 (ASTM D5127-90) or better at 10 gpm for cleaning of the Genesis solar wind payload for flight, in addition to sample handling tools for other astromaterials. This expansion included a 1000 gallon storage tank and several hundred feet of PVDF piping located in B. 31 and 31N. An extra reverse osmosis process unit was added to incoming water in 2005. Subsequently several major components were replaced, and in anticipation of adding new labs, an enlarged UPW system was designed in 2018 and completed in 2019. This system produces grade E1.1 UPW at 15 gpm in a continuous 1100 ft. loop with 5000 gal. storage tank, supplying 8 laboratories.

Ultrapure water↗

NASA NTRSDate not supplied

Comprehensive Analysis Aeroelastic Stability Predictions for the Mars Sample Recovery Helicopters and Ingenuity

A rotorcraft comprehensive analysis was used to generate aeroelastic stability predictions for both Ingenuity and the Mars Sample Recovery Helicopters. The analysis was performed multiple times for each rotor system with sequential increases to the level of modeling complexity. Initial cases used an independent blade analysis, trim inflow distribution, no airframe degrees of freedom, and a hover condition (a time-invariant problem). The final model used all blades, dynamic inflow, airframe degrees of freedom, and a Floquet theory approach to the flutter analysis. The approach of sequentially increasing modeling complexity was employed to ensure the causes of any observed instabilities could be identified. For each case, stability results were determined across a range of air densities, tip Mach numbers, and rotor collective pitch settings. For the Sample Recovery Helicopter, a forward flight condition analysis with the highest level of modeling complexity was also performed. The aeroelastic stability predictions showed both the Ingenuity and Sample Recovery Helicopter rotor systems to be stable for all analyzed conditions. Parameter excursions of structural properties were performed to explore conditions resulting in aeroelastic instabilities.

Aeroelastic↗

NASA NTRS2024

NASA's Capture, Containment, and Return System: Bringing Mars Samples to Earth

The Mars Sample Return (MSR) campaign is one of the most ambitious and complex planetary science exploration missions ever pursued. With the participation of NASA, ESA, and many industry partners, MSR aims to bring Martian rock and atmosphere samples to Earth with the goal of answering key questions about Mars’ geological, climatological and, potentially, biological evolution. To accomplish this ambitious goal, the MSR campaign relies on three distinct flight elements and a ground element. The Earth Return Orbiter mission that would host the Capture, Containment, and Return System (CCRS) is the last flight element of the trio. The mission would capture the orbiting sample in low Mars orbit (launched into orbit by another mission), contain it, and return it to Earth, landing at the Utah Test and Training Range. Since its early architecture, several changes were adopted by CCRS to improve overall payload efficiency and reduce mass. This paper will discuss the CCRS design, how the current CCRS architecture contributes to an improved mission concept, and the next critical steps of the mission toward its launch.

Mars mission↗

NASA NTRS2024

Enhancing the Protection of Shielded Thermal Protection Systems in Sample Return Spacecraft Against MMOD Impact

Sample return missions seek to collect samples from planets, moons, asteroids, and other planetary bodies and return them to earth for in-depth analysis. Backward planetary protection requirements are often in place for such missions to prevent the introduction of any extra-terrestrial material into the Earth’s biosphere, which could occur if a meteoroid or space debris particle were to damage a critical part of the returning spacecraft. The ability of a shielding system used to mitigate this damage risk is typically characterized by a ballistic limit equation (BLE), which predicts whether or not a protected system or structural element will sustain a critical failure due to a high-speed impact. In this paper, we develop a particle-impact-based BLE for the thermal protection system (TPS) of a sample return spacecraft that is protected by a multi-shock shield. The predictions of the BLE we develop for TPS failure are shown to be consistent with the predictions of hydrocode simulations.

Sample Return Spacecraft↗

NASA NTRSDate not supplied

Aeroheating Testing of the Mars Sample Return Earth Entry System with Surface Roughness

The Mars Sample Return Earth Entry System is a mission concept which would be the first NASA entry vehicle to utilize a woven thermal protection system as well as a 52.5 degree sphere-cone forebody geometry. Due to its novel design, historic experimental data sets were insufficient to validate the models used to characterize the expected aerothermal environment. A wind tunnel test campaign was funded by the Mars Sample Return Earth Entry System project in the NASA Langley 20-Inch Mach 6 Air Tunnel in order to obtain validation data. The December 2023 test entry of this campaign sought to obtain thermographic data to capture the impact of supersonic flow near woven surface roughness elements on convective heating augmentation, which was the largest remaining uncertainty in aerothermal design of the Earth Entry System. Data obtained were in family with prior results obtained on identical woven patterns without supersonic flow present at roughness elements, and it was found that this presence of supersonic flow did not have a noticeable effect on surface convective heating augmentation. Data were directly leveraged to support the NASA Mars Sample Return flight program and this data set is valuable for validating computational solutions on roughness-resolved grids.

Mars Sample Return↗

NASA NTRSDate not supplied

Aeroheating Testing of the Mars Sample Return Earth Entry System with Surface Roughness

Mars Sample Return↗