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NASA NTRS1984

Theoretical Modeling for Numerical Weather Prediction

The goal is to utilize predictability theory and numerical experimentation to identify and understand some of the dynamical processes which must be modeled more realistically if large-scale numerical weather predictions are to be improved. The emphasis is on the use of relatively simple models to exlore the properties of physically comprehensive general circulation models (GCM's). A global linear quasi-geostrophic model and the Goddard Laboratory for Atmospheric Sciences (GLAS) GCM were used to investigate several mechanisms which are responsible for the decay of large-scale forecast skill in mid-latitude numerical weather predictions. Five-day forecasts for an ensemble of cases were made using First GARP Global Experiment data. It was found that forecast skill depends crucially on the specification of the stationary forcing. A lack of stationary forcing leads to spurious westwad propagation of the ultralong waves. Forecasts made with stationary forcings derived from climatological data are superior to those using forcings inferred from observations immediately preceding the forecast period. Interhemispheric forecast differences were analyzed, and the model errors were compared to errors of a simple persistence-damped-to-climatology scheme and to errors of the GLAS GCM.

Somerville, R. C. J.↗
DOE OSTI2017

Effects of a Guide Field on the Larmor Electric Field and Upstream Electron Temperature Anisotropy in Collisionless Asymmetric Magnetic Reconnection

We perform the first study of the properties of the Larmor electric field (LEF) in collisionless asymmetric magnetic reconnection in the presence of an out-of-plane (guide) magnetic field for different sets of representative upstream parameters at Earth’s dayside magnetopause with an ion temperature greater than the electron temperature (the ion-to-electron temperature ratio fixed at 2) using two-dimensional particle-in-cell simulations. We show that the LEF does persist in the presence of a guide field. We study how the LEF thickness and strength change as a function of guide field and the magnetospheric temperature and reconnecting magnetic field strength. We find that the thickness of the LEF structure decreases, while its magnitude increases when a guide field is added to the reconnecting magnetic field. The added guide field makes the Larmor radius smaller, so the scaling with the magnetospheric ion Larmor radius is similar to that reported for the case without a guide field. Note, however, that the physics causing the LEF is not well understood, so future work in other parameter regimes is needed to fully predict the LEF for arbitrary conditions. We also find that a previously reported upstream electron temperature anisotropy arises in the vicinity of the LEF region both with and without a guide field. We argue that the generation of the anisotropy is linked to the existence of the LEF. The LEF can be used in combination with the electron temperature anisotropy as a signature to effectively identify dayside reconnection sites in observations.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2026

The detection of marine microseismic activity with the CUORE tonne-scale cryogenic experiment

Vibrations from experimental setups and the environment are a persistent source of noise for low-temperature calorimeters searching for rare events, including neutrinoless double beta ( 0νββ ) decay or dark matter interactions. Such noise can significantly limit experimental sensitivity to the physics case under investigation. Here, we report the detection of marine microseismic vibrations using mK-scale calorimeters. This study employs a multi-device analysis correlating data from CUORE, the leading experiment in the search for 0νββ decay with mK-scale calorimeters, and the Copernicus Earth Observation program, revealing the seasonal impact of Mediterranean Sea activity on CUORE’s energy thresholds, resolution, and sensitivity over four years. The detection of marine microseisms underscores the need to address faint environmental noise in ultra-sensitive experiments. Understanding how such noise couples to the detector and developing mitigation strategies is essential for next-generation experiments. We demonstrate one such strategy: a noise decorrelation algorithm implemented in CUORE using auxiliary sensors, which reduces vibrational noise and improves detector performance. Enhancing sensitivity to 0νββ decay and to rare events with low-energy signatures requires identifying unresolved noise sources, advancing noise reduction methods, and improving vibration suppression systems, all of which inform the design of next-generation rare event experiments.

experimental nuclear physics↗
DOE OSTI2024

Tracking the topology of neural manifolds across populations

Neural manifolds summarize the intrinsic structure of the information encoded by a population of neurons. Advances in experimental techniques have made simultaneous recordings from multiple brain regions increasingly commonplace, raising the possibility of studying how these manifolds relate across populations. However, when the manifolds are nonlinear and possibly code for multiple unknown variables, it is challenging to extract robust and falsifiable information about their relationships. We introduce a framework, called the method of analogous cycles, for matching topological features of neural manifolds using only observed dissimilarity matrices within and between neural populations. We demonstrate via analysis of simulations and in vivo experimental data that this method can be used to correctly identify multiple shared circular coordinate systems across both stimuli and inferred neural manifolds. Conversely, the method rejects matching features that are not intrinsic to one of the systems. Further, as this method is deterministic and does not rely on dimensionality reduction or optimization methods, it is amenable to direct mathematical investigation and interpretation in terms of the underlying neural activity. We thus propose the method of analogous cycles as a suitable foundation for a theory of cross-population analysis via neural manifolds.

97 MATHEMATICS AND COMPUTING↗
DOE OSTI2026

Effects of 9.5 Years of Whole-Soil Warming on the Fatty Acid and n-Alkanes Composition in Bulk Soil and Density Fractions at Blodgett Experimental Forest, California, USA

Original data of molecular data (fatty acids and n-alkanes) including concentrations and calculated molecular proxies in a whole-soil warming experiment at the Blodgett Forest Research Station after 9.5 years of warming. The study site has a Mediterranean climate with annual average temperature of 12.5 ℃ and annual average precipitation of 1774 mm. The study site is characterized by a mesic Ultic Alfisol formed from granitic parent material, corresponding to a Dystric Cambisol under the World Reference Base for Soil Resources (WRB) classification system. Experimental warming is applied throughout the soil profile to a depth of 1 m using vertically embedded heating cables that raise soil temperature by 4 °C relative to ambient conditions. Soil samples were collected on 1 May 2023, after the experiment had been operating continuously for about 9.5 years since its initiation in January 2014.The data has been processed from raw data and cross-validated by other peers. The dataset includes: - Bulk_Fattyacid_9.5-year_Soil_Warming_Blodgett, California, USA: fatty acid concentrations and proxies including Carbon Preference Index (CPI) and Average Chain Length (ACL) of bulk soil organic carbon; - Fractions_Fattyacid_9.5-year_Soil_Warming_Blodgett, California, USA: fatty acid concentrations and proxies including CPI and ACL of free particulate organic matter (fPOM) and mineral-associated organic matter (MAOM); - Bulk_Alkanes_9.5-year_Soil_Warming_Blodgett, California, USA: n-alkanes concentrations and proxies including CPI and ACL of bulk soil organic carbon; - Fractions_Alkanes_9.5-year_Soil_Warming_Blodgett, California, USA: n-alkanes concentrations and proxies including CPI and ACL of fPOM and MAOM; - n-Alkanes_All_Monomer_Concentration_9.5-year_Soil_Warming_Blodgett, California, USA: concentration of all the n-alkane monomers identified and integrated for bulk soil, fPOM and MAOM; - Fattyacid_All_Monomer_Concentration_9.5-year_Soil_Warming_Blodgett, California, USA: concentration of all the fatty acid monomers including diacids identified and integrated for bulk soil, fPOM, and MAOM. All data are provided in CSV format and can be viewed using Microsoft Excel. We specifically look at fatty acids (FA) and n-alkanes in bulk soil, fPOM and MAOM and calculated molecular proxies such as CPI and ACL to understand the source of oragnic carbon (with ACL) and degree of decomposition (CPI) of each soil fraction. Due to lack of long-chain fatty acids (carbon number ⩾ 20), microorganism-derived organic carbon is characterized by shorter ACL in comparison to plant-derived organic carbon. Fresh SOC is characterized by even-over-odd dominance for fatty acids and odd-over-even dominance for n-alkanes. Therefore, CPI indicates whether soil organic carbon (SOC) represents fresh input (CPI > 10) or is strongly decomposed (close to 1). The research questions should be then, after 9.5-year warming: 1. whether the relative contribution between microorganism-derived and plant-derived SOC in each soil fraction? 2. whether fPOM became more decomposed whereas MAOM remained relatively persistent in each soil fraction across the soil depth?

Carbon↗
NASA NTRS1992

Physical properties of Oxia/Lunae Planum and Arabia-type units in the central equatorial region of Mars

Classification and mapping of surficial units in the central equatorial region of Mars Consortium data identified the relatively dark but 'red' materials that cover Lunae Planum and surround the Meridiani materials of Oxia Palus inertia region of Arabia. Oxia Province consists of the regions dominated by the characteristically dark 'red' Oxia materials, but it includes darker streaks and splotches of relatively 'blue' Meridiani materials and brighter 'red' deposits of dust belonging to Eos Province (the bright 'red' border between Oxia and regional Meridiani deposits to the south) and Xanthe Province (the moderately bright 'red' dust deposits in western Chryse Planitia and its vicinity, including the VL-1 landing site). Two Oxia units were recognized: a darker unit present on Lunae Planum and east of Oxia Palus that have intermediate to high inertias. Oxia Province surrounds the extremely low thermal inertia Arabia Province in the east part of the study area, and occurs as isolated patches within Arabia (often including splotches of Meridiani materials within the Oxia patches). Arabia Province's materials have been widely interpreted as unconsolidated dust deposits which are currently forming at this stage of Mars' precessional climate cycle, although the persistence of stable, moderately strong albedo contracts among Arabia materials has not been addressed in those models. A systematic interpretation of Oxia and Arabia Province materials based on currently available remote sensing data is given.

Strickland, Edwin L., III↗
NASA NTRS2017

Evidence for Surface Water Ice in the Lunar Polar Regions Using Reflectance Measurements from the Lunar Orbiter Laser Altimeter and Temperature Measurements from the Diviner Lunar Radiometer Experiment

We find that the reflectance of the lunar surface within 5 deg of latitude of theSouth Pole increases rapidly with decreasing temperature, near approximately 110K, behavior consistent with the presence of surface water ice. The North polar region does not show this behavior, nor do South polar surfaces at latitudes more than 5 deg from the pole. This South pole reflectance anomaly persists when analysis is limited to surfaces with slopes less than 10 deg to eliminate false detection due to the brightening effect of mass wasting, and also when the very bright south polar crater Shackleton is excluded from the analysis. We also find that south polar regions of permanent shadow that have been reported to be generally brighter at 1064 nm do not show anomalous reflectance when their annual maximum surface temperatures are too high to preserve water ice. This distinction is not observed at the North Pole. The reflectance excursion on surfaces with maximum temperatures below 110K is superimposed on a general trend of increasing reflectance with decreasing maximum temperature that is present throughout the polar regions in the north and south; we attribute this trend to a temperature or illumination-dependent space weathering effect (e.g. Hemingway et al. 2015). We also find a sudden increase in reflectance with decreasing temperature superimposed on the general trend at 200K and possibly at 300K. This may indicate the presence of other volatiles such as sulfur or organics. We identified and mapped surfaces with reflectances so high as to be unlikely to be part of an ice-free population. In this south we find a similar distribution found by Hayne et al. 2015 based on UV properties. In the north a cluster of pixels near that pole may represent a limited frost exposure.

Emily A Fisher↗
DOE OSTI2013

Sulfur mustard induces an endoplasmic reticulum stress response in the mouse ear vesicant model

The endoplasmic reticulum (ER) stress response is a cell survival pathway upregulated when cells are under severe stress. Severely damaged mouse ear skin exposed to the vesicant, sulfur mustard (bis-2-chloroethyl sulfide, SM), resulted in increased expression of ER chaperone proteins that accompany misfolded and incorrectly made proteins targeted for degradation. Time course studies with SM using the mouse ear vesicant model (MEVM) showed progressive histopathologic changes including edema, separation of the epidermis from the dermis, persistent inflammation, upregulation of laminin γ2 (one of the chains of laminin-332, a heterotrimeric skin glycoprotein required for wound repair), and delayed wound healing from 24 h to 168 h post exposure. This was associated with time related increased expression of the cell survival ER stress marker, GRP78/BiP, and the ER stress apoptosis marker, GADD153/CHOP, suggesting simultaneous activation of both cell survival and non-mitochondrial apoptosis pathways. Dual immunofluorescence labeling of a keratinocyte migration promoting protein, laminin γ2 and GRP78/BIP, showed colocalization of the two molecules 72 h post exposure indicating that the laminin γ2 was misfolded after SM exposure and trapped within the ER. Taken together, these data show that ER stress is induced in mouse skin within 24 h of vesicant exposure in a defensive response to promote cell survival; however, it appears that this response is rapidly overwhelmed by the apoptotic pathway as a consequence of severe SM-induced injury. - Highlights: ► We demonstrated ER stress response in the mouse ear vesicant model. ► We described the asymmetrical nature of wound repair in the MEVM. ► We identified the distribution of various ER stress markers in the MEVM.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2019

Blackcomb2: Hardware-Software Co-design for Nonvolatile Memory in Exascale Systems

The impending shift from DRAM and Flash to fast nonvolatile memory (NVM) provides a game-changing opportunity to rethink traditional system architectures; to address their energy inefficiencies; to leverage the new devices for greater performance; and to exploit the new capability of nonvolatility to enhance system resilience, even in the face of larger system scale and degraded reliability of components. Building on the accomplishments of the Blackcomb Project, funded in 2010, we propose to identify, evaluate, and optimize the most promising NVM hardware and software technologies, which are essential to provide the necessary memory capacity, performance, resilience, and energy efficiency in exascale systems. Capacity and energy are the key drivers. Today's pressing application requirements mandate exascale computing; the datasets and concurrency of these applications demand memory capacity that cannot be met by traditional memory technologies. Although charge-based DRAM will endure, experts foresee only modest DRAM capacity gains. And, as moving data between levels of the hierarchy, and storing and accessing data on disk consumes significant energy, a more energy efficient solution is required. We, therefore, posit that exascale systems will need high density, energy-efficient storage technologies, such as nonvolatile memory for access, transformation, and management of exascale data. To address these issues, in this renewal request, we propose continue the work of our vertically- integrated, focussed team, where our co-design is informed by proxies and interactions with DOE co-design centers. In software, we start by assuming that nonvolatility requires new abstractions and new infrastructure, allowing persistent objects, and delivering performance gains for _le systems and robustness through fast checkpointing. In architecture, we evaluate the tradeoffs of performance and energy by employing a scalable and accurate simulation system to analyze realistic proxy applications. Through this co-design process, we rethink the whole memory architecture, from the cell to the array to the device, with ECC, wearout management, and interfaces. Finally, we provide open-source software models of these NVM-based memory systems.

97 MATHEMATICS AND COMPUTING↗
NASA NTRS2010

Combining Modis and Quikscat Data to Delineate Surface and Near-Surface Melt on the Greenland Ice Sheet

Over the last two decades, increasing melt has been measured on the Greenland Ice Sheet, along with mass loss as determined from satellite data, Monitoring the state of the Greenland Ice Sheet becomes critical especially because it is actively losing mass, and the ice sheet has a sea-level rise potential of 7 in. However measurement of the extent of surface melt varies depending on the sensor used, whether it is passive or active microwave or visible or thermal infrared. We have used remote-sensing data products to study surface and near-surface melt characteristics of the Greenland Ice Sheet. We present a blended MODIS-QS melt daily product for 2007 [1]. The products, including Moderate Resolution Imaging Spectroradiometer (MODIS) daily land-surface temperature (LST) and a special daily melt product derived from the QuikSCAT (QS) scatterometer [2,3] show consistency in delineating the melt boundaries on a daily basis in the 2007 melt season [I], though some differences are identified. An assessment of maximum melt area for the 2007 melt shows that the QSCAT product detects a greater amount of melt (862,769 square kilometers) than is detected by the MODIS LST product (766,184 square kilometers). The discrepancy is largely because the QS product can detect both surface and near-surface melt and the QS product captures melt if it occurred anytime during the day while the MODIS product is obtained from a point in time on a given day. However on a daily bases, other factors influence the measurement of melt extent. In this work we employ the digital-elevation model of Bamber et al. [4] along with the National Centers for Environmental Prediction (NCEP) data to study some areas and time periods in detail during the 2007 melt season. We focus on times in which the QS and MODIS LST products do not agree exactly. We use NCEP and elevation data to analyze the atmospheric factors forcing the melt process, to gain an improved understanding of the conditions that lead to melt and melt persistence, and our ability to capture surface melt accurately using MODIS and QS data.

Hall, Dorothy K.↗
NASA NTRS2020

Peter Pan Disks: Long-lived Accretion Disks Around Young M Stars

WISEA J080822.18–644357.3, an M star in the Carina association, exhibits extreme infrared excess and accretion activity at an age greater than the expected accretion disk lifetime. We consider J0808 as the prototypical example of a class of M star accretion disks at ages ≳20 Myr, which we call "Peter Pan" disks, because they apparently refuse to grow up. We present four new Peter Pan disk candidates identified via the Disk Detective citizen science project, coupled with Gaia astrometry. We find that WISEA J044634.16–262756.1 and WISEA J094900.65–713803.1 both exhibit significant infrared excess after accounting for nearby stars within the Two Micron All Sky Survey (2MASS) beams. The J0446 system has >95% likelihood of Columba membership. The J0949 system shows >95% likelihood of Carina membership. We present new Gemini Multi-Object Spectrograph optical spectra of all four objects, showing possible accretion signatures on all four stars. We present ground-based and TESS light curves of J0808 and 2MASS J0501–4337, including a large flare and aperiodic dipping activity on J0808, and strong periodicity on J0501. We find Paβ and Brγ emission indicating ongoing accretion in near-IR spectroscopy of J0808. Using observed characteristics of these systems, we discuss mechanisms that lead to accretion disks at ages ≳20 Myr, and find that these objects most plausibly represent long-lived CO-poor primordial disks, or "hybrid" disks, exhibiting both debris and primordial-disk features. The question remains: why have gas-rich disks persisted so long around these particular stars?

Steven M. Silverberg↗
DOE OSTI2016

The relationship between the plasmapause and outer belt electrons

Here, we quantify the spatial relationship between the plasmapause and outer belt electrons for a 5 day period, 15–20 January 2013, by comparing locations of relativistic electron flux peaks to the plasmapause. A peak-finding algorithm is applied to 1.8–7.7 MeV relativistic electron flux data. A plasmapause gradient finder is applied to wave-derived electron number densities >10 cm –3 . We identify two outer belts. Outer belt 1 is a stable zone of >3 MeV electrons located 1–2 R E inside the plasmapause. Outer belt 2 is a dynamic zone of <3 MeV electrons within 0.5 R E of the moving plasmapause. Electron fluxes earthward of each belt's peak are anticorrelated with cold plasma density. Belt 1 decayed on hiss timescales prior to a disturbance on 17 January and suffered only a modest dropout, perhaps owing to shielding by the plasmasphere. Afterward, the partially depleted belt 1 continued to decay at the initial rate. Belt 2 was emptied out by strong disturbance-time losses but restored within 24 h. For global context we use a plasmapause test particle simulation and derive a new plasmaspheric index F p , the fraction of a circular drift orbit inside the plasmapause. We find that the locally measured plasmapause is (for this event) a good proxy for the globally integrated opportunity for losses in cold plasma. Our analysis of the 15–20 January 2013 time interval confirms that high-energy electron storage rings can persist for weeks or even months if prolonged quiet conditions prevail. This case study must be followed up by more general study (not limited to a 5 day period).

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗
DOE OSTI2022

Tuning between continuous time crystals and many-body scars in long-range XYZ spin chains.

Persistent oscillatory dynamics in nonequilibrium many-body systems is a tantalizing manifestation of ergodicity breakdown that continues to attract much attention. Recent works have focused on two classes of such systems: discrete time crystals and quantum many-body scars (QMBS). While both systems host oscillatory dynamics, its origin is expected to be fundamentally different: discrete time crystal is a phase of matter which spontaneously breaks the Z 2 symmetry of the external periodic drive, while QMBS span a subspace of nonthermalizing eigenstates forming an su(2) algebra representation. Here, we ask a basic question: is there a physical system that allows us to tune between these two dynamical phenomena? In contrast to much previous work, we investigate the possibility of a continuous time crystal (CTC) in undriven, energy-conserving systems exhibiting prethermalization. We introduce a long-range XYZ spin model and show that it encompasses both a CTC phase as well as QMBS. We map out the dynamical phase diagram using numerical simulations based on exact diagonalization and time-dependent variational principle in the thermodynamic limit. We identify a regime where QMBS and CTC order coexist, and we discuss experimental protocols that reveal their similarities as well as key differences.

Bull, Kieran↗
DOE OSTI2021

C-FOG: Life of Coastal Fog

C-FOG is a comprehensive bi-national project dealing with the formation, persistence, and dissipation (life cycle) of fog in coastal areas (coastal fog) controlled by land, marine, and atmospheric processes. Given its inherent complexity, coastal-fog literature has mainly focused on case studies, and there is a continuing need for research that integrates across processes (e.g., air–sea–land interactions, environmental flow, aerosol transport, and chemistry), dynamics (two-phase flow and turbulence), microphysics (nucleation, droplet characterization), and thermodynamics (heat transfer and phase changes) through field observations and modeling. Central to C-FOG was a field campaign in eastern Canada from 1 September to 8 October 2018, covering four land sites in Newfoundland and Nova Scotia and an adjacent coastal strip transected by the Research Vessel Hugh R. Sharp . An array of in situ, path-integrating, and remote sensing instruments gathered data across a swath of space–time scales relevant to fog life cycle. Satellite and reanalysis products, routine meteorological observations, numerical weather prediction model (WRF and COAMPS) outputs, large-eddy simulations, and phenomenological modeling underpin the interpretation of field observations in a multiscale and multiplatform framework that helps identify and remedy numerical model deficiencies. An overview of the C-FOG field campaign and some preliminary analysis/findings are presented in this paper.

58 GEOSCIENCES↗
DOE OSTI2026

Seasonal variation of the surface cross-shelf exchange in the northern South China Sea: a Lagrangian perspective

Previous studies on cross-shelf exchange, predominantly adopted an Eulerian perspective, struggled to identify water sources and pathways. Using a high-resolution regional ocean modeling system (ROMS) and Lagrangian particle tracking, this study systematically investigates the seasonal variation and dynamics of surface cross-shelf exchange in the northern South China Sea (NSCS) from a Lagrangian perspective. Based on daily released 30-day drifter trajectories we identify the key pathways, source regions for surface cross-shelf exchange, revealing pronounced seasonal variability. Results show the surface cross-shelf exchange generally following monsoon-driven Ekman transport. However, local dynamics, especially topographic modulation, can outweigh the expected Ekman-driven transport, producing surface exchange patterns opposite to that predicted from the prevailing winds. Topographic effects vary across different scales. In the coastal waters of western Guangdong during winter, despite downwelling-favorable winds, the modulation of alongshore currents by island topography induces an eastward pressure gradient. This gradient, through geostrophic balance, drives offshore flows opposite to wind-driven Ekman onshore transport. Furthermore, the eastern widened shelf exhibits a distinct seasonal variation of cross-shelf exchange, with strong offshore transport (opposite to the direction of Ekman transport) in winter and exceedingly weak exchange in summer. Analysis of the underlying mechanisms reveals that this winter offshore transport is primarily attributed to geostrophic flows driven by surface pressure gradient that is jointly modulated by the Kuroshio intrusion and local widened shelf topography, and enhanced by cumulative submesoscale processes. In summer, a persistent and strong along-isobath jet acts as a dynamic barrier, effectively suppressing the exchange. These findings highlight the important role of topography in regulating surface material transport, and have important implications for predicting the advection and dispersion of plankton or oil spills over the continental shelf influenced by monsoon.

Hao, Dongliang↗
DOE OSTI2012

Neuregulin-1 is neuroprotective in a rat model of organophosphate-induced delayed neuronal injury

Current medical countermeasures against organophosphate (OP) nerve agents are effective in reducing mortality, but do not sufficiently protect the CNS from delayed brain damage and persistent neurological symptoms. In this study, we examined the efficacy of neuregulin-1 (NRG-1) in protecting against delayed neuronal cell death following acute intoxication with the OP diisopropylflurophosphate (DFP). Adult male Sprague–Dawley rats were pretreated with pyridostigmine (0.1 mg/kg BW, i.m.) and atropine methylnitrate (20 mg/kg BW, i.m.) prior to DFP (9 mg/kg BW, i.p.) intoxication to increase survival and reduce peripheral signs of cholinergic toxicity but not prevent DFP-induced seizures or delayed neuronal injury. Pretreatment with NRG-1 did not protect against seizures in rats exposed to DFP. However, neuronal injury was significantly reduced in most brain regions by pretreatment with NRG-1 isoforms NRG-EGF (3.2 μg/kg BW, i.a) or NRG-GGF2 (48 μg/kg BW, i.a.) as determined by FluroJade-B labeling in multiple brain regions at 24 h post-DFP injection. NRG-1 also blocked apoptosis and oxidative stress-mediated protein damage in the brains of DFP-intoxicated rats. Administration of NRG-1 at 1 h after DFP injection similarly provided significant neuroprotection against delayed neuronal injury. These findings identify NRG-1 as a promising adjuvant therapy to current medical countermeasures for enhancing neuroprotection against acute OP intoxication. -- Highlights: ► NRG-1 blocked DFP induced neuronal injury. ► NRG-1 did not protect against seizures in rats exposed to DFP. ► NRG-1 blocked apoptosis and oxidative stress in the brains of DFP-intoxicated rats. ► Administration of NRG-1 at 1 h after DFP injection prevented delayed neuronal injury.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2017

Cell-type specific role of the RNA-binding protein, NONO, in the DNA double-strand break response in the mouse testes

Here, the tandem RNA recognition motif protein, NONO, was previously identified as a candidate DNA double-strand break (DSB) repair factor in a biochemical screen for proteins with end-joining stimulatory activity. Subsequent work showed that NONO and its binding partner, SFPQ, have many of the properties expected for bona fide repair factors in cell-based assays. Their contribution to the DNA damage response in intact tissue in vivo has not, however, been demonstrated. Here we compare DNA damage sensitivity in the testes of wild-type mice versus mice bearing a null allele of the NONO homologue (Nono gt ). In wild-type mice, NONO protein was present in Sertoli, peritubular myoid, and interstitial cells, with an increase in expression following induction of DNA damage. As expected for the product of an X-linked gene, NONO was not detected in germ cells. The Nono gt/0 mice had at most a mild testis developmental phenotype in the absence of genotoxic stress. However, following irradiation at sublethal, 2–4 Gy doses, Nono gt/0 mice displayed a number of indicators of radiosensitivity as compared to their wild-type counterparts. These included higher levels of persistent DSB repair foci, increased numbers of apoptotic cells in the seminiferous tubules, and partial degeneration of the blood-testis barrier. There was also an almost complete loss of germ cells at later times following irradiation, evidently arising as an indirect effect reflecting loss of stromal support. Results demonstrate a role for NONO protein in protection against direct and indirect biological effects of ionizing radiation in the whole animal.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2023

The influence of hydrothermal synthesis temperature on the electronic structure and luminescence property of Cr-doped ZnGa 2 O 4 nanoparticles

Cr-doped ZnGa 2 O 4 (CZGO) is a near-infrared-emitting material with long-lasting persistent luminescence. This unique property makes it a great candidate in optical imaging and sensing for various biomedical applications. Nanosized CZGO can be synthesized via a hydrothermal approach, but the reaction temperatures reported in existing literature vary from 120°C to 220°C, approaching the highest temperature allowed for a standard autoclave vessel. The reason for choosing a particular hydrothermal synthesis temperature has rarely been discussed, and it is unclear whether the resulting CZGO possess the same optical properties. This work compares CZGO nanoparticles synthesized at temperatures from the lowest reported 120°C and to as high as 220°C. We find that although all the synthesized CZGO are light emitting, they exhibit different luminescence intensities and respond differently to the change of excitation energy. X-ray absorption fine structure (XAFS) analysis is employed to investigate the local chemical environment around Zn, Ga and Cr, respectively. We found synthesis temperature strongly influences the Ga species formed in these particles. The energy transfer mechanism is further elucidated using X-ray excited optical luminescence (XEOL) in combination with element-specific XAFS. Two energy transfer paths are identified, which explains the different excitation energy dependencies of the observed photoluminescence.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗