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At least 109 records · Page 6

Effect of Electrical Stimulation on Beta-Adrenergic Receptor Population and Cyclic AMP Production in Chicken and Rat Skeletal Muscle Cell Cultures

Expression of the beta-adrenergic receptor (PAR) and its coupling to Adenosine 3'5' Cyclic Monophosphate (cAMP) synthesis are important components of the signaling system that controls muscle atrophy and hypertrophy and the goal of this study was to determine if electrical stimulation in a pattern simulating slow muscle contraction would alter the PAR response in primary cultures of avian and mammalian skeletal muscle cells. Specifically chicken skeletal muscle cells and rat skeletal muscle cells that had been grown for 7 d in culture, were subjected to electrical stimulation for an additional 2 d at a pulse frequency of 0.5 pulses/sec and a pulse duration of 200 msec. In chicken skeletal muscle cells, the PAR population was not significantly affected by electrical stimulation; however, the ability, of these cells to synthesize cyclic AMP was reduced by approximately one-half. In contrast, the PAR population in rat muscle cells was increased slightly but not significantly by electrical stimulation, and the ability of these cells to synthesize cyclic AMP was increased by almost twofold. The basal levels of intracellular cyclic AMP in neither rat muscle cells nor chicken muscle cells were affected by electrical stimulation.

Young, Ronald B.↗

Effect of electrical stimulation on beta-adrenergic receptor population and cyclic amp production in chicken and rat skeletal muscle cell cultures

Expression of the beta-adrenergic receptor (betaAR) and its coupling to cyclic AMP (cAMP) synthesis are important components of the signaling system that controls muscle atrophy and hypertrophy, and the goal of this study was to determine if electrical stimulation in a pattern simulating slow muscle contraction would alter the betaAR response in primary cultures of avian and mammalian skeletal muscle cells. Specifically, chicken skeletal muscle cells and rat skeletal muscle cells that had been grown for 7 d in culture were subjected to electrical stimulation for an additional 2 d at a pulse frequency of 0.5 pulses/sec and a pulse duration of 200 msec. In chicken skeletal muscle cells, the betaAR population was not significantly affected by electrical stimulation; however, the ability of these cells to synthesize cyclic AMP was reduced by approximately one-half. In contrast, the betaAR population in rat muscle cells was increased slightly but not significantly by electrical stimulation, and the ability of these cells to synthesize cyclic AMP was increased by almost twofold. The basal levels of intracellular cyclic AMP in neither rat muscle cells nor chicken muscle cells were affected by electrical stimulation.

Muscle, Skeletal/cytology↗

A study of stall deterrent systems for general aviation aircraft

Stall deterrent concepts for general aviation aircraft have been investigated using simulation studies and flight test experiments. It was found that the simulator was suitable for the development of deterrent system concepts, but the simulator was unacceptable for pilot evaluation of system effectiveness under typical stall/spin accident conditions. A Cessna 319 was outfitted with sensors, servoactuators, and analog control logic necessary to investigate a wide range of stall deterrent systems. It was found that an acoustic stall sensor and an error control law were sufficient for stall deterrence. The pitch intervention control system prevented aircraft stall for all aircraft configurations and pilot inputs. The variable up elevator stop concept was found to be effective for slow decelerations to aircraft stall. The stall deterrent systems were evaluated by four professional pilots and three low time pilots.

Bennett, A. G.↗

Single step optimization of manipulator maneuvers with variable structure control

One step ahead optimization has been recently proposed for spacecraft attitude maneuvers as well as for robot manipulator maneuvers. Such a technique yields a discrete time control algorithm implementable as a sequence of state-dependent, quadratic programming problems for acceleration optimization. Its sensitivity to model accuracy, for the required inversion of the system dynamics, is shown in this paper to be alleviated by a fast variable structure control correction, acting between the sampling intervals of the slow one step ahead discrete time acceleration command generation algorithm. The slow and fast looping concept chosen follows that recently proposed for optimal aiming strategies with variable structure control. Accelerations required by the VSC correction are reserved during the slow one step ahead command generation so that the ability to overshoot the sliding surface is guaranteed.

Chen, N.↗

Self-tuning control of attitude and momentum management for the Space Station

This paper presents a hybrid state-space self-tuning design methodology using dual-rate sampling for suboptimal digital adaptive control of attitude and momentum management for the Space Station. This new hybrid adaptive control scheme combines an on-line recursive estimation algorithm for indirectly identifying the parameters of a continuous-time system from the available fast-rate sampled data of the inputs and states and a controller synthesis algorithm for indirectly finding the slow-rate suboptimal digital controller from the designed optimal analog controller. The proposed method enables the development of digitally implementable control algorithms for the robust control of Space Station Freedom with unknown environmental disturbances and slowly time-varying dynamics.

Shieh, L. S.↗

Adaptive Control of a Transport Aircraft Using Differential Thrust

The paper presents an adaptive control technique for a damaged large transport aircraft subject to unknown atmospheric disturbances such as wind gust or turbulence. It is assumed that the damage results in vertical tail loss with no rudder authority, which is replaced with a differential thrust input. The proposed technique uses the adaptive prediction based control design in conjunction with the time scale separation principle, based on the singular perturbation theory. The application of later is necessitated by the fact that the engine response to a throttle command is substantially slow that the angular rate dynamics of the aircraft. It is shown that this control technique guarantees the stability of the closed-loop system and the tracking of a given reference model. The simulation example shows the benefits of the approach.

Stepanyan, Vahram↗

Drive Motor Improved for 8- by 6-Foot Supersonic Wind Tunnel/9- by 15-Foot Low-Speed Wind Tunnel Complex

An operational change made recently in the drive motor system for the 8- by 6-Foot Supersonic Wind Tunnel (8x6 SWT)/9- by 15-Foot Low-Speed Wind Tunnel (9x15 LSWT) complex resulted in dramatic power savings and expanded operating range. The 8x6 SWT/9x15 LSWT complex offers a unique combination of wind tunnel conditions for both high- and low-speed testing. Prior to the work discussed in this article, the 8- by 6-ft test section offered airflows ranging from Mach 0.36 to 2.0. Subsonic testing was done in the 9-ft high, 15-ft wide test area in the return leg of the facility. The air speed in this test section can range from 0 to 175 mph (Mach 0.23). In the past, we varied the air speed by using a combination of the compressor speed and the position of the tunnel flow-control doors. When very slow speeds were required in the 9x15 LSWT, these large tunnel flow control doors might be very nearly full open, bleeding off large quantities of air, even with the drive system operating at its previous minimum speed of about 510 rpm. Power drawn during this mode of operation varied between 15 and 18 MW/hr, but clearly much of this power was not being used to provide air that would be used for testing in the test section. The air exiting these large doors represented wasted power. Early this year, the facility's tunnel drive system was run on one motor instead of three to see if lower drive speeds could be achieved that would, in turn, result in large power savings because unnecessary air would not be blown out of the flow-control doors unnecessarily. In addition, if the drive could be run slower, then slower speeds would also be possible in the 8x6 SWT test section as an added benefit. Results of the first tests performed early last year showed that in fact the drive, when operating on only one motor, actually reached a steady-state speed of only 337 rpm and drew an amazingly small 6 MW/hr of electrical power. During daytime operation of the drive, this meant that it would be possible to save as much as 10 MW/hr, or nearly $600 per hour of operation, for many of the 9x15 LSWT's testing regimes. An added benefit of this power-saving venture was that since the 8x6 SWT and 9x15 LSWT are indeed on a common loop, if the compressor is slowed down to benefit the 9x15 LSWT, then the air moving through the 8x6 SWT is also moving slower than ever before. In fact, testing has proven that the 8x6 SWT can now achieve Mach 0.25, whereas its previous lower limit was Mach 0.36. This added benefit has attracted additional customers

Source record↗

Command and Control Software Development Memory Management

This internship was initially meant to cover the implementation of unit test automation for a NASA ground control project. As is often the case with large development projects, the scope and breadth of the internship changed. Instead, the internship focused on finding and correcting memory leaks and errors as reported by a COTS software product meant to track such issues. Memory leaks come in many different flavors and some of them are more benign than others. On the extreme end a program might be dynamically allocating memory and not correctly deallocating it when it is no longer in use. This is called a direct memory leak and in the worst case can use all the available memory and crash the program. If the leaks are small they may simply slow the program down which, in a safety critical system (a system for which a failure or design error can cause a risk to human life), is still unacceptable. The ground control system is managed in smaller sub-teams, referred to as CSCIs. The CSCI that this internship focused on is responsible for monitoring the health and status of the system. This team's software had several methods/modules that were leaking significant amounts of memory. Since most of the code in this system is safety-critical, correcting memory leaks is a necessity.

Programmin↗

Experiment Document Information System (EDIS) evolution

The EDIS is the second generation of a system designed to produce and control a document containing large amounts of text in combination with tables and graphs of mathematical/scientific data. The first generation system proved the concept, but the slow, unfriendly user interface resulted in an effort to find an off-the-shelf product to improve the interface capability while maintaining the system requirements. The basic design of that first system was combined with the hypertext concepts inherent in HyperCard to generate the most usable EDIS. Currently in the latter stages of design, the EDIS promises to be the first step in the automation of the process required for defining complete packages of Life Sciences experiments for the Shuttle missions.

Moorhead, Jane↗

Coolant Leak from ISS External Active Thermal Control System (EATCS) – An Examination of Most Probable

The Port (P1) and Starboard (S1) External Active Thermal Control Systems (EATCS) are single phase, mechanically pumped ammonia loops that operate independently to cool majority of the hardware and payloads onboard the International Space Station (ISS). A slow ammonia leak was detected using pressure and quantity telemetry five years after the P1 EATCS was activated. The leak gradually accelerated to a rate that locating and isolating the leak became imperative to maintain cooling capability. Partial pressure measurements from the Robotic External Leak Locator (RELL) scan surveys narrowed the search to the supply and return jumpers connecting one of three radiators to the system. Subsequently, the ISS crew performed high definition video surveys during an Extravehicular Activity (EVA), or spacewalk, and ammonia flakes were observed projecting from the jumpers. Thus, the ground teams were confident that the culprit of the ammonia leak were the jumpers. The ammonia leak stopped after ground teams remotely isolated and vented those jumpers and associated radiator. Both jumpers were removed and returned to the ground, and a root cause investigation was conducted. A calibrated leak test determined the bulk of the ammonia leaked through a pair of seals in a Quick Disconnect (QD), or connector, on one end of the return jumper. The return jumper QD was dissected, visually inspected, chemically tested and evaluated. The results indicated the most probable cause of the accelerating ammonia leak was due to defective seals, plating delamination underneath the seals, and on-orbit thermal cycles exacerbating the delamination. Both jumpers were refurbished, relaunched to the ISS, and scheduled to be reinstalled during an EVA in 2022. It appeared the issue was unique, but recently the S1 EATCS is showing signs of an accelerating ammonia leak, and RELL scans narrowed the source to a similar pair of radiator jumpers.

Thermal Control↗

Inherent spiral stability in a fixed wing aircraft by means of a simplified pneumatic wing tip control system

The concept of the lateralizer device is to sense the difference in wing tip static pressures (differential pressures being created by wing tip venturis) produced when any deviation from straight to level flight occurs. In a steady state turn the low slow wing tip experiences less venturi suction than the high fast wing tip. This signal activates the appropriate servos connected to the ailerons to produce a wing leveling restoring moment. If the wing is subjected to rolling velocities due to changes of the local angle of attack at the tip, the downgoing wing tip operates at a higher angle than the upgoing one. If with an increase in the angle of attack the signal is such that it increases the venturi pressure, then the servos are activated to produce the most negative signal to an upgoing aileron which results in a wing leveling restoring moment. In other words, any deviation from straight and level flight in either roll or yaw is exploited and the difference signal from the differential pressures activates the servo to effect or produce the necessary corrective action to null the signal.

Goglia, G. L.↗

Growth of Three Lettuce Cultivars in NASA's HDU PEM During the 2010 DRATS Test

NASA's 2010 Desert Research and Technology Studies (DRATS) of the VEGGIE Food Production System in the Habitat Demonstration Unit (HDU) Pressurized Excursion Module (PEM) was the first operational evaluation of salad crop production technology in a NASA analog test. Rooting media and slow release fertilizers were evaluated for three lettuce cultivars that had shown promise as candidates for a surface based food production system. These tests involved comparing growth, color and quality of the lettuce cultivars grown under VEGGIE LED array (Orbitec, Madison, WI) or Biomass Production System for Education ((BSEe), Orbitec, Madison, WI) compact fluorescent lamps using a gravity feed water delivery system. Mission relevant conditions of CO2, temperature and RH were maintained using controlled environment chambers (EGC, Chagrin Falls, OH). Growth data was obtained for the two red leaf lettuce cultivars, Outredgeous and Firecracker, and the green Bibb lettuce cultivar, Flandria. Growth and quality was evaluated using different concentrations (7.5 g/L and 15 g/L) of commercial slow release fertilizer (Osmocote Plus 15-9-12, Scotts, Maryville, OH) and Nutricote 18-6-8 (Florikan, Sarasota, FL) in either a peat/vermiculite media (sunshine LP5 MiX, Sungro, Bellview, WA) or calcined montmorillonite clay [(arcillite,)Turface Proleague, Profile LLC, Buffalo Grove, IL]. The commercial peat/vermiculite mix generally resulted in larger plants than those grown in arcillite. Increasing the concentration of Osmocote from 7.5 to 15 g/L increased the height, dry mass, and leaf area of lettuce cultivars. In contrast, there was a decrease in growth parameters when concentration of Nutricote was increased from 7.5 to 15 g/L. The best growth was obtained with the 7.5 g/L Nutricote using a commercial peat/vermiculite mixture. This media was used for field testing VEGGIE plant system in the 2010 DRAT test. The VEGGIE nutrient delivery system worked well, was able to be maintained by multiple operators with a minimum of training, and supported excellent lettuce growth for the duration of the 14-day test. The operational DRAT field testing in the HDU identified light quality issues related to morphology and pigment development that will need to be addressed through additional testing. Feedback from the crew, ground support personnel, and human factors leads was uniformly positive on the psychological value of having the crop production system in the pressurized excursion module. Data are being used to design a plant atrium with LED lighting to evaluate salad crop growth during NASA's 2011 DRATS test.

Stutte, Gary W.↗

Coolant Leak from ISS External Active Thermal Control System (EATCS) – An Examination of Most Probable

The Port (P1) and Starboard (S1) External Active Thermal Control Systems (EATCS) are single phase, mechanically pumped ammonia loops that operate independently to cool the majority of the hardware and payloads onboard the International Space Station (ISS). A slow ammonia leak was detected using pressure and quantity telemetry five years after the P1 EATCS was activated. The leak gradually accelerated to a rate that locating and isolating the leak became imperative to maintain cooling capability. Partial pressure measurements from the Robotic External Leak Locator (RELL) scan surveys narrowed the search to the supply and return jumpers connecting one of three radiators to the system. Subsequently, the ISS crew performed high definition video surveys during an Extravehicular Activity (EVA), or spacewalk, and ammonia flakes were observed projecting from the jumpers. Thus, the ground teams were confident that the culprit for the ammonia leak was the jumpers. The ammonia leak stopped after ground teams remotely isolated and vented those jumpers and associated radiator. Both jumpers were removed and returned to the ground, and a root cause investigation was conducted. A calibrated leak test determined the bulk of the ammonia leaked through a pair of seals in a Quick Disconnect (QD), or connector, on one end of the return jumper. The return jumper QD was dissected, visually inspected, chemically tested and evaluated. The results indicated the most probable cause of the accelerating ammonia leak was due to defective seals, plating delamination underneath the seals, and on-orbit thermal cycles exacerbating the delamination. Both jumpers were refurbished, relaunched to the ISS, and scheduled to be reinstalled during an EVA in 2022. It appeared the issue was unique, but recently the S1 EATCS is showing signs of an accelerating ammonia leak, and RELL scans narrowed the source to a similar pair of radiator jumpers.

Thermal Control↗

Flight testing a V/STOL aircraft to identify a full-envelope aerodynamic model

Flight-test techniques are being used to generate a data base for identification of a full-envelope aerodynamic model of a V/STOL fighter aircraft, the YAV-8B Harrier. The flight envelope to be modeled includes hover, transition to conventional flight and back to hover, STOL operation, and normal cruise. Standard V/STOL procedures such as vertical takeoff and landings, and short takeoff and landings are used to gather data in the powered-lift flight regime. Long (3 to 5 min) maneuvers which include a variety of input types are used to obtain large-amplitude control and response excitations. The aircraft is under continuous radar tracking; a laser tracker is used for V/STOL operations near the ground. Tracking data are used with state-estimation techniques to check data consistency and to derive unmeasured variables, for example, angular accelerations. A propulsion model of the YAV-8B's engine and reaction control system is used to isolate aerodynamic forces and moments for model identification. Representative V/STOL flight data are presented. The processing of a typical short takeoff and slow landing maneuver is illustrated.

Mcnally, B. David↗

Touchdown: The Development of Propulsion Controlled Aircraft at NASA Dryden

This monograph relates the important history of the Propulsion Controlled Aircraft project at NASA's Dryden Flight Research Center. Spurred by a number of airplane crashes caused by the loss of hydraulic flight controls, a NASA-industry team lead by Frank W. Burcham and C. Gordon Fullerton developed a way to land an aircraft safely using only engine thrust to control the airplane. In spite of initial skepticism, the team discovered that, by manually manipulating an airplane's thrust, there was adequate control for extended up-and-away flight. However, there was not adequate control precision for safe runway landings because of the small control forces, slow response, and difficulty in damping the airplane phugoid and Dutch roll oscillations. The team therefore conceived, developed, and tested the first computerized Propulsion Controlled Aircraft (PCA) system. The PCA system takes pilot commands, uses feedback from airplane measurements, and computes commands for the thrust of each engine, yielding much more precise control. Pitch rate and velocity feedback damp the phugoid oscillation, while yaw rate feedback damps the Dutch roll motion. The team tested the PCA system in simulators and conducted flight research in F-15 and MD-11 airplanes. Later, they developed less sophisticated variants of PCA called PCA Lite and PCA Ultralite to make the system cheaper and therefore more attractive to industry. This monograph tells the PCA story in a non- technical way with emphasis on the human aspects of the engineering and flic,ht-research effort. It thereby supplements the extensive technical literature on PCA and makes the development of this technology accessible to a wide audience.

Tucker, Tom↗

Traveling-wave maser, closed cycle refrigerator automation

At the present time all traveling-wave maser and closed-cycle refrigerator system (TWM-CCR) and their power supply and helium compressor assemblies are operated manually. Many man-hours are required of highly trained and experienced operators, resulting in high operator cost. Of greater importance are the problems of unpredictable failure and long downtimes. Since field performance is not monitored, system degradation is detected only when it has progressed to the point of catastrophic failure. Without the aid of performance histories, failure diagnosis is arduous and slow. Rational and functional requirements for the TWM-CCR Automation project are the following: (1) to improve reliability and reduce downtime by providing unattended monitor and control, alarm and fault location, failure prediction and self-corrective action; (2) to reduce operator cost by providing automatic tuning and cooldown procedures; and (3) to improve system operations and development by providing the needed engineering data.

Mcneil, J. A.↗

Radar Waveform Pulse Analysis Measurement System for High-Power GaN Amplifiers

This work presents a measurement system to characterize the pulsed response of high-power GaN amplifiers for use in space-based SAR platforms that require very strict amplitude and phase stability. The measurement system is able to record and analyze data on three different time scales: fast, slow, and long, which allows for greater detail of the mechanisms that impact amplitude and phase stability. The system is fully automated through MATLAB, which offers both instrument control capability and in-situ data processing. To validate this system, a high-power GaN HEMT amplifier operated in saturation was characterized. The fast time results show that variations to the amplitude and phase are correlated to DC supply transients, while long time characteristics are correlated to temperature changes.

high-power amplifier (HPA)↗

Clocked Thrust Reversers

An aircraft includes a fuselage including a propulsion system supported within an aft portion. A thrust reverser is mounted proximate to the propulsion system for directing thrust in a direction to slow the aircraft. The thrust reverser directs thrust at an angle relative to a vertical plane to reduce interference on control surfaces and reduce generation of underbody lift.

Suciu, Gabriel L.↗