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At least 109 records · Page 6

Covariance of biophysical data with digital topograpic and land use maps over the FIFE site

This paper discusses the biophysical stratification of the FIFE site, implementation of the stratification utilizing geographic information system methods, and validation of the stratification with respect to field measurements of biomass, Bowen ratio, soil moisture, and the greenness vegetation index (GVI) derived from TM satellite data. Maps of burning and topographic position were significantly associated with variation in GVI, biomass, and Bowen ratio. The stratified design did not significantly alter the estimated site-wide means for surface climate parameters but accounted for between 25 and 45 percent of the sample variance depending on the variable.

Davis, F. W.↗

Adjustment of footprint correction for airborne flux mapping over the FIFE site

A meaningful interpretation of airborne flux estimates over nonuniform terrain must consider local advection from nonhomogeneous source distributions (footprint correction). An empirical procedure for footprint correction of airborne flux data obtained in the First ISLSCP Field Experiment (FIFE) 1989 is presented, based on simple analytical solutions of the diffusion equation which have been adjusted to approximate more realistic solutions. Optimized estimates of surface flux 'maps' derived from airborne observations are then constructed for maximum correlation between flux estimates and independently observed surface characteristics. This paper also includes an analysis of resolution for the given procedure, in terms of amplitude recovery of hypothetical square-wave distributions of surface flux density, and discusses its implications for the interpretation of the optimized estimates of FIFE surface flux maps. Results show that corrected spatial sink distributions are obtained by the current empirical procedure at the cost of considerable reduction in amplitude recovery of small-scale variations in surface flux density. The high spatial correlation observed between corrected flux maps and surface characteristics such as greenness or surface temperature excess is ascribed to the approximately correct positioning of the main flux gradients across the site, which can be expected to have been reproduced with amplitude recovery equal to or greater than 60 percent.

Schuepp, P. H.↗

Mapping Neutralizing and Immunodominant Sites on the SARS-CoV-2 Spike Receptor-Binding Domain by Structure-Guided High-Resolution Serology

We report analysis of the specificity and kinetics of neutralizing antibodies (nAbs) elicited by SARS-CoV-2 infection is crucial for understanding immune protection and identifying targets for vaccine design. In a cohort of 647 SARS-CoV-2-infected subjects, we found that both the magnitude of Ab responses to SARS-CoV-2 spike (S) and nucleoprotein and nAb titers correlate with clinical scores. The receptor-binding domain (RBD) is immunodominant and the target of 90% of the neutralizing activity present in SARS-CoV-2 immune sera. Whereas overall RBD-specific serum IgG titers waned with a half-life of 49 days, nAb titers and avidity increased over time for some individuals, consistent with affinity maturation. We structurally defined an RBD antigenic map and serologically quantified serum Abs specific for distinct RBD epitopes leading to the identification of two major receptor-binding motif antigenic sites. Our results explain the immunodominance of the receptor-binding motif and will guide the design of COVID-19 vaccines and therapeutics.

59 BASIC BIOLOGICAL SCIENCES↗

The uses of ERTS-1 imagery in the analysis of landscape change

The author has identified the following significant results. The analysis of strip mining from ERTS-1 data has resulted in the mapping of landscape changes for the Cumberland Plateau Test Site. Several mapping experiments utilizing ERTS-1 data have been established for the mapping of state-wide land use regions. The first incorporates 12 frames of ERTS-1 imagery for the generalized thematic mapping of forest cover for the state of Tennessee. In another mapping effort, 14 ERTS-1 images have been analyzed for plowed ground signatures to produce a map of agricultural regions for Tennessee, Kentucky, and the northern portions of Mississippi and Alabama. Generalized urban land use categories and transportation networks have been determined from ERTS-1 imagery for the Knoxville Test Site. Finally, through the analysis of ERTS-1 imagery, short-lived phenomena such as the 1973 spring floods on the Mississippi River in western Tennessee, have been detected, monitored, and mapped.

Rehder, J. B.↗

A calcium-dependent protein kinase can inhibit a calmodulin-stimulated Ca2+ pump (ACA2) located in the endoplasmic reticulum of Arabidopsis

The magnitude and duration of a cytosolic Ca(2+) release can potentially be altered by changing the rate of Ca(2+) efflux. In plant cells, Ca(2+) efflux from the cytoplasm is mediated by H(+)/Ca(2+)-antiporters and two types of Ca(2+)-ATPases. ACA2 was recently identified as a calmodulin-regulated Ca(2+)-pump located in the endoplasmic reticulum. Here, we show that phosphorylation of its N-terminal regulatory domain by a Ca(2+)-dependent protein kinase (CDPK isoform CPK1), inhibits both basal activity ( approximately 10%) and calmodulin stimulation ( approximately 75%), as shown by Ca(2+)-transport assays with recombinant enzyme expressed in yeast. A CDPK phosphorylation site was mapped to Ser(45) near a calmodulin binding site, using a fusion protein containing the N-terminal domain as an in vitro substrate for a recombinant CPK1. In a full-length enzyme, an Ala substitution for Ser(45) (S45/A) completely blocked the observed CDPK inhibition of both basal and calmodulin-stimulated activities. An Asp substitution (S45/D) mimicked phosphoinhibition, indicating that a negative charge at this position is sufficient to account for phosphoinhibition. Interestingly, prior binding of calmodulin blocked phosphorylation. This suggests that, once ACA2 binds calmodulin, its activation state becomes resistant to phosphoinhibition. These results support the hypothesis that ACA2 activity is regulated as the balance between the initial kinetics of calmodulin stimulation and CDPK inhibition, providing an example in plants for a potential point of crosstalk between two different Ca(2+)-signaling pathways.

NASA Discipline Plant Biology↗

Inspection and Mapping of Savannah River Site (SRS) Waste Tanks via Unmanned Aircraft System (UAS) – 25351

The CSTF at SRS contain 51 waste tanks with 8 closed waste tanks between FTF and HTF. SRMC is the LW contractor. The LW mission includes removing legacy nuclear waste from these tanks and treating it for final disposition. Once the bulk of the waste has been removed from a tank, it will undergo inspection and sampling to characterize the remaining waste in the tank prior to it being operationally closed. There are multiple points in the tank closure process where an inspection is performed, and there are multiple parts of a tank that get inspected. Waste tanks have a primary containment vessel (referred to as the “Primary”) and a secondary containment vessel (referred to as the “Annulus”) that surrounds the primary. Both of these sections of a tank receive multiple inspections throughout the closure process.

Murphy, Lucas D. [Savannah River Mission Completio↗

Geology of six possible Martian landing sites

Thirteen detailed maps of the first six (of ten) possible sites for a future Lander/Rover/Sample-return mission to Mars are either nearing completion or in review. Basic mosaic of all sites were available at 1:2 million scale. In addition to preparing new base maps, special enhancements were generated for computer mosaics and individual images that delineate the geologic units more clearly. Photoclinometric profiles that allowed measurement of the thicknesses of geologic units, the depth of channels, and the throw on faults were prepared. Crater counts were made for each geologic unit.

Masursky, Harold↗

A Streamlined and Standardized Benthic Habitat Mapping Approach for Marine and Hydrokinetic Site Environmental Assessments

We have combined two seafloor mapping techniques to efficiently map benthic habitat conditions. Multibeam echosounder (MBES) surveys provide detailed bathymetry and backscatter mosaics. Sediment profile and plan view imaging (SPI/PV) rapidly provides dense ground truth data on seabed physical and biological features. A key innovation of this project has been the development of a computer-assisted image processing system that standardizes the SPI data generation process. A unique combination of machine- and deep-learning techniques for grain-size classification, sediment–water interface delineation, and object detection (e.g., infauna and feeding voids) is described. An automated SPI image analysis system provides a standardized and cost-effective approach for image processing. Combining MBES with SPI/PV camera technology provides a first tier approach to rapidly documenting seabed physical and biological conditions in high resolution at MHK sites. When coupled with computer automated data extraction from the imagery, this approach provides a standardized and cost-effective method to establish and monitor benthic habitat conditions over time.

Benthic Habitat Maping, Sediment-Profile Imaging, ↗

Correlation of LANDSAT lineaments with Devonian gas fields in Lawrence County, Ohio

In an effort to locate sources of natural gas in Ohio, the fractures and lineaments in Black Devonian shale were measured by: (1) field mapping of joints, swarms, and fractures; (2) stereophotointerpretation of geomorphic lineaments with precise photoquads; and (3) by interpreting the linear features on LANDSAT images. All results were compiled and graphically represented on 1:250,000 scale maps. The geologic setting of Lawrence County was defined and a field fracture map was generated and plotted as rose patterns at the exposure site. All maps were compared, contrasted, and correlated by superimposing each over the other as a transparency. The LANDSAT lineaments had significant correlation with the limits of oil and gas producing fields. These limits included termination of field production as well as extensions to other fields. The lineaments represent real rock fractures with zones of increased permeability in the near surface bedrock.

Johnson, G. O.↗

Application of remote sensor data to geologic analysis of the Bonanza Test Site Colorado

A geologic map of the Bonanza Test Site is nearing completion. Using published large scale geologic maps from various sources, the geology of the area is being compiled on a base scaled at 1:250,000. Sources of previously published geologic mapping include: (1) USGS Bulletins; (2) professional papers and geologic quadrangle maps; (3) Bureau of Mines reports; (4) Colorado School of Mines quarterlies; and (5) Rocky Mountain Association of Geologist Guidebooks. This compilation will be used to evaluate ERTS, Skylab, and remote sensing underflight data.

Lee, K.↗

UNF-ST&DARDS Enhancements for RCCA Data in As-loaded Dual Purpose Cask Models

This report summarizes the work performed to enable detailed modeling of rod cluster control assembly (RCCAs) in dual purpose canisters (DPCs) in as-loaded configurations using the Used Nuclear Fuel – Storage, Transportation & Disposal Analysis Resource and Data System (UNF-ST&DARDS). The goal of this project was to evaluate the reactivity impact that RCCAs have on k eff of DPCs with pressurized water reactor (PWR) fuel to potentially use the additional margin in future post-closure criticality safety analysis. This preliminary evaluation determined the number of DPCs that currently require compensatory actions prior to emplacement in a repository because of their high reactivity under post-closure criticality scenarios, which could be made acceptable by including the as-loaded RCCAs as specified in the Unified Database (UDB). This report briefly describes the modeling methods currently used within UNF-ST&DARDS and the modifications made to automate inclusion of the as-loaded RCCAs in the DPCs for post-closure criticality calculations for the loss of neutron absorber (NA) scenarios, or NA models. For the Zion site, the loss of basket scenarios, or degraded basket (DB) models, are also included. Discussion is also provided regarding the UDB data compared to available site-specific loading map data for the sites, which are specifically evaluated herein. This report compares the modifications to a similar evaluation for the Zion by site conducted by Walker as an initial validation. After the partial validation, all existing PWR sites with applicable NA models within the UDB were evaluated.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗

Use of Thematic Mapper for water quality assessment

The evaluation of simulated TM data obtained on an ER-2 aircraft at twenty-five predesignated sample sites for mapping water quality factors such as conductivity, pH, suspended solids, turbidity, temperature, and depth, is discussed. Using a multiple regression for the seven TM bands, an equation is developed for the suspended solids. TM bands 1, 2, 3, 4, and 6 are used with logarithm conductivity in a multiple regression. The assessment of regression equations for a high coefficient of determination (R-squared) and statistical significance is considered. Confidence intervals about the mean regression point are calculated in order to assess the robustness of the regressions used for mapping conductivity, turbidity, and suspended solids, and by regressing random subsamples of sites and comparing the resultant range of R-squared, cross validation is conducted.

Horn, E. M.↗

Image Analysis for Facility Siting: a Comparison of Lowand High-altitude Image Interpretability for Land Use/land Cover Mapping

For two test sites in Pennsylvania the interpretability of commercially acquired low-altitude and existing high-altitude aerial photography are documented in terms of time, costs, and accuracy for Anderson Level II land use/land cover mapping. Information extracted from the imagery is to be used in the evaluation process for siting energy facilities. Land use/land cover maps were drawn at 1:24,000 scale using commercially flown color infrared photography obtained from the United States Geological Surveys' EROS Data Center. Detailed accuracy assessment of the maps generated by manual image analysis was accomplished employing a stratified unaligned adequate class representation. Both 'area-weighted' and 'by-class' accuracies were documented and field-verified. A discrepancy map was also drawn to illustrate differences in classifications between the two map scales. Results show that the 1:24,000 scale map set was more accurate (99% to 94% area-weighted) than the 1:62,500 scale set, especially when sampled by class (96% to 66%). The 1:24,000 scale maps were also more time-consuming and costly to produce, due mainly to higher image acquisition costs.

Borella, H. M.↗

Mapping the Temperature-dependent and network site-specific onset of spectral diffusion at the surface of a water cluster cage

We explore the kinetic processes that sustain equilibrium in a microscopic, finite system. This is accomplished by monitoring the spontaneous, time-dependent frequency evolution (the frequency autocorrelation) of a single OH oscillator, embedded in a water cluster held in a temperature-controlled ion trap. The measurements are carried out by applying two-color, IR-IR photodissociation mass spectrometry to the D3O+?(HDO)(D2O)19 isotopologue of the “magic number” protonated water cluster, H+?(H2O)21. The OH group can occupy any one of the five spectroscopically distinct sites in the distorted pentagonal dodecahedron cage structure. The OH frequency is observed to evolve over tens of milliseconds in the temperature range (90-120 K). Starting at 100 K, large “jumps” are observed between two OH frequencies separated by ~300 cm-1 indicating migration of the OH group from the bound OH site at 3350 cm-1 to the free position at 3686 cm-1. Increasing the temperature to 110 K leads to partial interconversion among many sites. All sites are observed to interconvert at 120 K such that the distribution of the unique OH group among them adopts the form one would expect for a canonical ensemble. The spectral dynamics displayed by the clusters thus offer an unprecedented view into the molecular-level processes that drive spectral diffusion in an extended network of water molecules.

Yang, Nan↗