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101 records · Page 6

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NASA NTRS2023

What’s That Supposed to Mean? Capturing Micro-Behaviors in Teams

Future long-duration space exploration (LDSE) crews will require extensive coordination, cooperation, and team functioning as they face a myriad of challenges rooted in both taskwork and teamwork (Bell et al., 2015; Landon et al., 2018). While exposed to extreme conditions, crew members must navigate living and working together in prolonged confinement. Moreover, astronaut teams are becoming increasingly diverse, introducing significant variability in team composition. This increasing diversity, alongside traditional constraints of LDSE, introduces additional challenges into effective team functioning. To date, most methods for capturing team functioning rely on self-report measures. Such measures are prone to several limitations, including but not limited to social desirability bias, halo effect, and leniency effects (Trull & Ebner-Priemer, 2013), which skew data and limit nuanced understandings of phenomena at play. Self-report measures broadly capture team functioning, lending the nature of such methods to identifying underlying “macro”-behaviors (i.e., behaviors that are long-standing and last over time). However, team functioning is far more complex than a series of macro-behaviors, rendering reliance on self-report data deficient for accurate measurement. Recent research demonstrates the potential of alternative methods for capturing team functioning, such as speech and physiological data (Chaffin et al., 2017; Murray & Oertel, 2018). Consequently, these methods are more suitable for capturing micro-behaviors: brief, often unconscious expressions that affect the extent to which an individual feels included by others around them (Paletz et al., 2013). Micro-behaviors can be further classified into microaggressions (i.e., subtle, negative exchanges; Keller & Galgay, 2010) or micro-affirmations (i.e., subtle, positive exchanges; Kyte et al. 2020), both of which influence team functioning. Due to the subtle nature of micro-behaviors, contextual factors have a significant impact when determining if it is aggressive or affirmative. Additionally, several iterations of microbehaviors can have lingering effects on team interactions. For example, the use of “mm-hmm” by a crew member can function as both a micro-affirmation and micro-aggression. Specifically, it can be indication of active listening (i.e., micro-affirmation) or as an expression of annoyance (i.e., aggression) depending on the context in which it occurs. Auditory features (e.g., tone, frequency) can help delineate between the two forms; however, the contextual factors (e.g., previous interactions between team members, crew demographics) add a layer of complexity that render auditory features alone as insufficient to capture micro-behaviors. Consequently, this paper seeks to provide a novel approach in which multi-modal data (i.e., auditory features and contextual features) are used in a random-forest model to better identify distinguishing characteristics between micro-affirmations and micro-aggressions. In turn, detected micro-behaviors are used to predict team performance, thereby demonstrating the value of capturing micro-behaviors as supplemental data to macro-behaviors.

Sydney R. Begerowski↗

NASA NTRS2023

What’s That Supposed to Mean? Capturing Micro-Behaviors in Teams

Future long-duration space exploration (LDSE) crews will require extensive coordination, cooperation, and team functioning as they face a myriad of challenges rooted in both taskwork and teamwork (Bell et al., 2015; Landon et al., 2018). While exposed to extreme conditions, crew members must navigate living and working together in prolonged confinement. Moreover, astronaut teams are becoming increasingly diverse, introducing significant variability in team composition. This increasing diversity, alongside traditional constraints of LDSE, introduces additional challenges into effective team functioning. To date, most methods for capturing team functioning rely on self-report measures. Such measures are prone to several limitations, including but not limited to social desirability bias, halo effect, and leniency effects (Trull & Ebner-Priemer, 2013), which skew data and limit nuanced understandings of phenomena at play. Self-report measures broadly capture team functioning, lending the nature of such methods to identifying underlying “macro”-behaviors (i.e., behaviors that are long-standing and last over time). However, team functioning is far more complex than a series of macro-behaviors, rendering reliance on self-report data deficient for accurate measurement. Recent research demonstrates the potential of alternative methods for capturing team functioning, such as speech and physiological data (Chaffin et al., 2017; Murray & Oertel, 2018). Consequently, these methods are more suitable for capturing micro-behaviors: brief, often unconscious expressions that affect the extent to which an individual feels included by others around them (Paletz et al., 2013). Micro-behaviors can be further classified into micro-aggressions (i.e., subtle, negative exchanges; Keller & Galgay, 2010) or micro-affirmations (i.e., subtle, positive exchanges; Kyte et al. 2020), both of which influence team functioning. Due to the subtle nature of micro-behaviors, contextual factors have a significant impact when determining if it is aggressive or affirmative. Additionally, several iterations of micro-behaviors can have lingering effects on team interactions. For example, the use of “mm-hmm” by a crew member can function as both a micro-affirmation and micro-aggression. Specifically, it can be indication of active listening (i.e., micro-affirmation) or as an expression of annoyance (i.e., aggression) depending on the context in which it occurs. Auditory features (e.g., tone, frequency) can help delineate between the two forms; however, the contextual factors (e.g., previous interactions between team members, crew demographics) add a layer of complexity that render auditory features alone as insufficient to capture micro-behaviors. Consequently, this paper seeks to provide a novel approach in which multi-modal data (i.e., auditory features and contextual features) are used in a random-forest model to better identify distinguishing characteristics between micro-affirmations and micro-aggressions. In turn, detected micro-behaviors are used to predict team performance, thereby demonstrating the value of capturing micro-behaviors as supplemental data to macro-behaviors.

Sydney Begerowski↗

NASA NTRS1996

Characterizing photosynthesis and transpiration of plant communities in controlled environments

CO2 and water vapor fluxes of hydroponically grown wheat and soybean canopies were measured continuously in several environments with an open gas exchange system. Canopy CO2 fluxes reflect the photosynthetic efficiency of a plant community, and provide a record of plant growth and health. There were significant diurnal fluctuations in root and shoot CO2 fluxes, and in shoot water vapor fluxes. Canopy stomatal conductance (Gc) to water vapor was calculated from simultaneous measurements of canopy temperature (Tcan) and transpiration rates (Tr). Tr in the dark was substantial, and there were large diurnal fluctuations in both Gc and Tr. Canopy net Photosynthesis (Pnet), Tr, and Gc increased with increasing net radiation. Gc increased with Tr, suggesting that the stomata of plants in controlled environments (CEs) behave differently from field-grown plants. A transpiration model based on measurements of Gc was developed for CEs. The model accurately predicted Tr from a soybean canopy.

NASA Discipline Life Support Systems↗

NASA NTRS1996

Monitoring the Photosynthetic Apparatus During Space Flight: Interspecific Variation in Chlorophyll Fluorescence Signatures Induced by Different Root Zone Stresses

Chlorophyll fluorescence has been used extensively as a tool to indicate stress to the photosynthetic apparatus in green plants. A rise in fluorescence has been attributed to the blockage of photosystem II photochemistry, and patterns of fluorescence decay (quenching) from dark adapted leaves can be related to specific photochemical and non-photochemical deexcitation pathways of light trapped by the photosynthetic apparatus and thus result in characteristically different fluorescence signatures. Four distantly related plant species, Hypocharis radicata (Asteraceae), Brassica rapa (Brassicaceae), Spinacea oleracea (Chenopodiaceae) and Triticum aestivum (Poaceae), were grown hydroponically for three weeks before the initiation of three different root zone stresses (10 mM Cu, 100 mM NaCl and nitrogen deficient nutrition). After 10 days, characteristic fluorescence signatures for each stress could be noted although the degree varied between species. Fast kinetics analysis showed a reduction in plastoquinone pool size for copper and nitrogen stress for all species but a more species specific result with NaCl stress. Photochemical quenching kinetics varied between species and stress treatments from no quenching in S. oleracea in copper treatments to increased photochemical quenching in NaCl treatments. Non-photochemical quenching kinetics demonstrated a distinct pattern between stresses for all species. Copper treatments characteristically exhibited a shallow, flat non-photochemical quenching profile suggesting a general blockage of electron transport whereas NaCl treatments exhibited a slow rising profile that suggested damage to thylakoid acidification kinetics and nitrogen deficiency exhibited a fast rising and declining profile that suggested an altered state 1-state 2 transition regulated by the phosphorylation of LHCII. These results demonstrate characteristic fluorescence signatures for specific plant stresses that may be applied to different, unrelated plant species. In addition, the potential use of chlorophyll fluorescence for both basic research and diagnostic physiology in space biology is presented.

Bubenheim, David L.↗

NASA NTRS2006

High-frequency ECG

The standard ECG is by convention limited to 0.05-150 Hz, but higher frequencies are also present in the ECG signal. With high-resolution technology, it is possible to record and analyze these higher frequencies. The highest amplitudes of the high-frequency components are found within the QRS complex. In past years, the term "high frequency", "high fidelity", and "wideband electrocardiography" have been used by several investigators to refer to the process of recording ECGs with an extended bandwidth of up to 1000 Hz. Several investigators have tried to analyze HF-QRS with the hope that additional features seen in the QRS complex would provide information enhancing the diagnostic value of the ECG. The development of computerized ECG-recording devices that made it possible to record ECG signals with high resolution in both time and amplitude, as well as better possibilities to store and process the signals digitally, offered new methods for analysis. Different techniques to extract the HF-QRS have been described. Several bandwidths and filter types have been applied for the extraction as well as different signal-averaging techniques for noise reduction. There is no standard method for acquiring and quantifying HF-QRS. The physiological mechanisms underlying HF-QRS are still not fully understood. One theory is that HF-QRS are related to the conduction velocity and the fragmentation of the depolarization wave in the myocardium. In a three-dimensional model of the ventricles with a fractal conduction system it was shown that high numbers of splitting branches are associated with HF-QRS. In this experiment, it was also shown that the changes seen in HF-QRS in patients with myocardial ischemia might be due to the slowing of the conduction velocity in the region of ischemia. This mechanism has been tested by Watanabe et al by infusing sodium channel blockers into the left anterior descending artery in dogs. In their study, 60 unipolar ECGs were recorded from the entire ventricular surface and were signal-averaged and filtered in the 30-250 Hz frequency range. The results showed that the decrease noted in the HF-QRS correlated linearly with the local conduction delay. The results suggest that HF-QRS is a potent indicator of disturbed local conduction. An alternative theory is that HF-QRS reflect the shape of the original electrocardiographic signal. Bennhagen et al showed that root mean square (RMS) voltage values of the depolarization signal correlate poorly with the signal amplitude but highly with the first and second derivatives, i.e. the velocity and the acceleration of the signal. It has also been suggested that the autonomic nervous system affects HF-QRS. For example, sitting up causes significant changes in HF-QRS in some leads compared to the supine position [Douglas et al., 2006]. Unpublished results indicate that familial dysautonomic patients (both vagal and sympathetic degeneration) have very little Reduced Amplitude Zones (RAZ) formation . Athletic individuals, especially elite athletes, who have vagally-mediated changes on the conventional ECG (i.e. early repolarization, bradycardia) have increased RAZ formation. Further electrophysiological studies are needed, however, to better understand the underlying mechanisms of HF-QRS. Several investigators have studied HF-QRS in different cardiac conditions, including acute myocardial ischemia and myocardial infarction (MI). However, in order for clinicians to confidently use HF-QRS as an adjunct to standard ECG, more knowledge about the characteristics of HF-QRS is needed.

Tragardh, Elin↗

NASA NTRS1990

Toward applied behavior analysis of life aloft

This article deals with systems at multiple levels, at least from cell to organization. It also deals with learning, decision making, and other behavior at multiple levels. Technological development of a human behavioral ecosystem appropriate to space environments requires an analytic and synthetic orientation, explicitly experimental in nature, dictated by scientific and pragmatic considerations, and closely approximating procedures of established effectiveness in other areas of natural science. The conceptual basis of such an approach has its roots in environmentalism which has two main features: (1) knowledge comes from experience rather than from innate ideas, divine revelation, or other obscure sources; and (2) action is governed by consequences rather than by instinct, reason, will, beliefs, attitudes or even the currently fashionable cognitions. Without an experimentally derived data base founded upon such a functional analysis of human behavior, the overgenerality of "ecological systems" approaches render them incapable of ensuring the successful establishment of enduring space habitats. Without an experimentally derived function account of individual behavioral variability, a natural science of behavior cannot exist. And without a natural science of behavior, the social sciences will necessarily remain in their current status as disciplines of less than optimal precision or utility. Such a functional analysis of human performance should provide an operational account of behavior change in a manner similar to the way in which Darwin's approach to natural selection accounted for the evolution of phylogenetic lines (i.e., in descriptive, nonteleological terms). Similarly, as Darwin's account has subsequently been shown to be consonant with information obtained at the cellular level, so too should behavior principles ultimately prove to be in accord with an account of ontogenetic adaptation at a biochemical level. It would thus seem obvious that the most productive conceptual and methodological approaches to long-term research investments focused upon human behavior in space environments will require multidisciplinary inputs from such wide-ranging fields as molecular biology, environmental physiology, behavioral biology, architecture, sociology, and political science, among others.

Review↗

NASA NTRS1995

Anti-dopamine beta-hydroxylase immunotoxin-induced sympathectomy in adult rats

Anti-dopamine beta-hydroxylase immunotoxin (DHIT) is an antibody-targeted noradrenergic lesioning tool comprised of a monoclonal antibody against the noradrenergic enzyme, dopamine beta-hydroxylase, conjugated to saporin, a ribosome-inactivating protein. Noradrenergic-neuron specificity and completeness and functionality of sympathectomy were assessed. Adult, male Sprague-Dawley rats were given 28.5, 85.7, 142 or 285 micrograms/kg DHIT i.v. Three days after injection, a 6% to 73% decrease in the neurons was found in the superior cervical ganglia of the animals. No loss of sensory, nodose and dorsal root ganglia, neurons was observed at the highest dose of DHIT. In contrast, the immunotoxin, 192-saporin (142 micrograms/kg), lesioned all three ganglia. To assess the sympathectomy, 2 wk after treatment (285 micrograms/kg), rats were anesthetized with urethane (1 g/kg) and cannulated in the femoral artery and vein. DHIT-treated animals' basal systolic blood pressure and heart rate were significantly lower than controls. Basal plasma norepinephrine levels were 41% lower in DHIT-treated animals than controls. Tyramine-stimulated release of norepinephrine in DHIT-treated rats was 27% of controls. Plasma epinephrine levels of DHIT animals were not reduced. DHIT-treated animals exhibited a 2-fold hypersensitivity to the alpha-adrenergic agonist phenylephrine. We conclude that DHIT selectively delivered saporin to noradrenergic neurons resulting in destruction of these neurons. Anti-dopamine beta-hydroxylase immunotoxin administration produces a rapid, irreversible sympathectomy.

NASA Discipline Regulatory Physiology↗

NASA NTRS2002

Interactions between gravitropism and phototropism in plants

To receive adequate light and nutrients for survival, plants orient stems and stem-like organs toward light and away from the gravity vector and, conversely, orient roots into the soil, away from light toward the direction of gravity. Therefore, both gravity and light can influence the differential growth of plant organs. To add to the complexity of the interactions between gravity and light, each stimulus can enhance or reduce the effectiveness of the other. On earth, the constant presence of gravity makes it difficult to determine whether plant growth and development is influenced by gravity or light alone or the combination of the two stimuli. In the past decade, our understanding of the gravity and light transduction pathways has advanced through the use of mutants in either gravitropic or phototropic responses and the use of innovative techniques that reduce the effects of one stimulus on the other. Thus, both unique and common elements in the transduction pathways of the gravitropic and phototropic responses have been isolated. This article is focused on the interactions between the light- and gravity-transduction pathways and describes methods used to separate the influences of these two environmental stimuli.

NASA Discipline Plant Biology↗

NASA NTRS2001

Effect of spaceflight on isoflavonoid accumulation in etiolated soybean seedlings

In order to explore the potential impact of microgravity on flavonoid biosynthesis, we examined isoflavonoid levels in soybean (Glycine max) tissues generated under both spaceflight and clinorotation conditions. A 6-day Space Shuttle-based microgravity exposure resulted in enhanced accumulation of isoflavone glycosides (daidzin, 6"-O-malonyl-7-O-glucosyl daidzein, genistin, 6"-O-malonyl-7-O-glucosyl genistein) in hypocotyl and root tissues, but reduced levels in cotyledons (relative to 1g controls on Earth). Soybean seedlings grown on a horizontally rotating clinostat for 3, 4 and 5 days exhibited (relative to a vertical clinorotation control) an isoflavonoid accumulation pattern similar to the space-grown tissues. Elevated isoflavonoid levels attributable to the clinorotation treatment were transient, with the greatest increase observed in the three-day-treated tissues and smaller increases in the four- and five-day-treated tissues. Differences between stresses presented by spaceflight and clinorotation and the resulting biochemical adaptations are discussed, as is whether the increase in isoflavonoid concentrations were due to differential rates of development under the "gravity" treatments employed. Results suggest that spaceflight exposure does not impair isoflavonoid accumulation in developing soybean tissues and that isoflavonoids respond positively to microgravity as a biochemical strategy of adaptation.

Flight Experiment↗

NASA NTRS1995

Assessing potential targets of calcium action in light-modulated gravitropism

Light, through the mediation of the pigment phytochrome, modulates the gravitropic response of the shoots and roots of many plants. The transduction of both light and gravity stimuli appears to involve Ca(2+)-regulated steps, one or more of which may represent points of intersection between the two transduction chains. To be confident that Ca2+ plays a critical role in stimulus-response coupling for gravitropism, it will be important to identify specific targets of Ca2+ action whose function can be clearly linked to the regulation of growth. Calcium typically exerts its influence on cell metabolism through binding to and activating key regulatory proteins. The three best characterized of these proteins in plants are the calmodulins, calcium-dependent protein kinases, and annexins. In this review we summarize what is known about the structure and function of these proteins and speculate on how their activation by Ca2+ could influence the differential growth response of gravitropism.

Review, Tutorial↗

NASA NTRS1998

Abnormalities of the QT interval in primary disorders of autonomic failure

BACKGROUND: Experimental evidence shows that activation of the autonomic nervous system influences ventricular repolarization and, therefore, the QT interval on the ECG. To test the hypothesis that the QT interval is abnormal in autonomic dysfunction, we examined ECGs in patients with severe primary autonomic failure and in patients with congenital dopamine beta-hydroxylase (DbetaH) deficiency who are unable to synthesize norepinephrine and epinephrine. SUBJECTS AND METHODS: Maximal QT and rate-corrected QT (QTc) intervals and adjusted QTc dispersion [(maximal QTc - minimum QTc on 12 lead ECG)/square root of the number of leads measured] were determined in blinded fashion from ECGs of 67 patients with primary autonomic failure (36 patients with multiple system atrophy [MSA], and 31 patients with pure autonomic failure [PAF]) and 17 age- and sex-matched healthy controls. ECGs of 5 patients with congenital DbetaH deficiency and 6 age- and sex-matched controls were also analyzed. RESULTS: Patients with MSA and PAF had significantly prolonged maximum QTc intervals (492+/-58 ms(1/2) and 502+/-61 ms(1/2) [mean +/- SD]), respectively, compared with controls (450+/-18 ms(1/2), P < .05 and P < .01, respectively). A similar but not significant trend was observed for QT. QTc dispersion was also increased in MSA (40+/-20 ms(1/2), P < .05 vs controls) and PAF patients (32+/-19 ms(1/2), NS) compared with controls (21+/-5 ms(1/2)). In contrast, patients with congenital DbetaH deficiency did not have significantly different RR, QT, QTc intervals, or QTc dispersion when compared with controls. CONCLUSIONS: Patients with primary autonomic failure who have combined parasympathetic and sympathetic failure have abnormally prolonged QT interval and increased QT dispersion. However, QT interval in patients with congenital DbetaH deficiency was not significantly different from controls. It is possible, therefore, that QT abnormalities in patients with primary autonomic failure are not solely caused by lesions of the sympathetic nervous system, and that the parasympathetic nervous system is likely to have a modulatory role in ventricular repolarization.

Non-NASA Center↗