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At least 109 records · Page 6

Isophotal diameters of galaxies at high redshifts

New ultraviolet observations and archival spectra are presented for the bright disk of the galaxy NGC 4736 to derive the ultraviolet surface brightness profile. This is used to predict the appearance of such a galaxy at high redshift. The profiles in red light of 100 galaxies determined by S. Kent (1984) are used with assumed ultraviolet spectra to predict the isophotal diameters of these galaxies as a function of redshift. The results show that, for ground-based imagery, normal spiral galaxies should be resolvable to the largest redshifts (z roughly 1). For space-based imagery, much smaller but brighter galactic disks characterized by extensive star formation will be those resolved at the highest redshifts (z roughly 2.5).

Weedman, Daniel W.↗

Allegheny Observatory search for planetary systems

The newly developed Multichannel Astrometric Photometer (MAP) and a rebuilt red light 0.76 meter Thaw refractor are used in an astrometric observational program to detect Jupiter-like planets in orbit about nearby stars. The program includes 15 stars and is obtaining approx. 12 good observations per year of each of them, sufficient to assure an annual normal point precision of 0.001 arcsec (1 mas) per object. The observational program will yield the first astrometric information about planetary systems in general. The astrometric technique is most sensitive to nearby planetary systems and to massive planets that have orbits that place them within the regions around a star where the temperature is sufficiently low to permit the existence of water ice grains. Thus it covers a different search space than that of radial velocity techniques. Currently the only astrometric survey program, it is complementary to other detection programs and some target stars have been included to assure overlap with them. The minimum detectable mass varies with the particular target star from objects almost twice as massive as Neptune to bodies almost twice as massive as Jupiter.

Gatewood, George D.↗

Orbit crowding of molecular gas at a bar-spiral arm transition zone in M83

The southwestern bar-spiral arm transition zone in M83 is been studied in CO, H-alpha, H I, red light, and the radio continuum. A massive molecular gas complex in the heart of the transition zone is composed or two principal components which have the morphology and kinematics expected from orbit crowding, where gas on highly elliptical orbits form the bar region converges with gas on more circular orbits from the spiral arm region. Three mechanisms for the origin of the orbit crowding are investigated, and it is proposed that the crowding is due primarily to density wave streaming motions caused by the bar and spiral arms. The inner CO component is partially coincident with a region of highly polarized radio continuum emission which precedes the H-alpha spiral arm by 15-25 arcsec, indicating that it lies on or just downstream from a shock front. This suggests that the bar gas approaching the transition zone is shocked and explains the ridge of dense gas seen upstream from the spiral arm.

Kenney, Jeffrey D. P.↗

Differentiation and Tropisms in Space-Grown Moss

This grant supported a Space Shuttle experiment on the effects of microgravity on moss cells. Moss provides a rich system for gravitational and spaceflight research. The early phase of the moss life cycle consists of chains of cells that only grow only at their tips. In the moss Ceratodon purpureus these filaments (protonemata) grow away from gravity in the dark, in a process called gravitropism. The tipmost cells, the apical cells, contain heavy starch-filled bodies called amyloplasts that probably function in g-sensing and that sediment within the apical cell. The SPM-A (Space Moss aka SPAM) experiment flew in November - December, 1997 on STS-87 as part of the Collaborative US Ukrainian Experiment (CLTE). The experiment was accommodated in hardware purpose-built by NASA KSC and Bionetics and included Petri Dish Fixation Units (PDFU) and BRIC-LEDs. Together, this hardware allowed for the culture of the moss on agar in commercial petri dishes, for unilateral illumination with red light of varying intensity, and for chemical fixation in situ. The key findings of the spaceflight were quite unexpected. Neither the orientation of tip-growth nor the distribution of amyloplasts was random in microgravity.

Sack, Fred D.↗

Response of Grape Leaf Spectra to Phylloxera Infestation

During the 1993 growing season, leaf reflectance and chlorophyll concentrations were monitored with respect to phylloxera (root-louse) infestation in a Napa Valley (California) vineyard. Study plots were established in areas of severely infested, mildly infested, and uninfested sections of the vineyard. A handheld chlorophyll meter, measuring leaf transmittance of near-infrared and red light, confirmed that reduced foliar chlorophyll concentrations were symptomatic of phylloxera stress in the sample vines. Bidirectional reflectance measurements of green and near-infrared light, taken on fresh leaves with a laboratory spectrophotometer, were related to chlorophyll concentration but did not allow discrimination of mildly infested from uninfested vines.

Johnson, Lee F.↗

Estimating the Single-Trial Characteristics of Event-Related Responses: Evaluation of the MCERP Algorithm

Single-trial event-related responses collected during the course of an experiment are typically averaged before analysis resulting in a rather crude picture of event-related brain dynamics. It has been quite clear for some time that these responses exhibit trial-to-trial variability: however, the computational techniques necessary to deal with such responses in noisy conditions have not been available. To this end we have developed the multiple-component, event-related potential model (mcERP), which assumes that the each event-related response consists of a sum of multiple evoked components each described by a stereotypical waveshape. These waveshapes are allowed to vary in amplitude and onset latency from trial to trial, which allows us to capture, to first-order, the trial-dependent variations in event-related brain dynamics. We have constructed many sets of synthetic data designed to simulate intracortical recordings from a 15 channel, linear-array multielectrode implanted acutely in V1 of an awake-behaving macaque undergoing visual stimulation with a red light flash. This synthetic data was used to characterize the performance of the mcERP algorithm. First we quantified the degree to which such trial-to-trial variability aids in the identification of multiple components, and we demonstrate that amplitude variability is a more important factor in component separation than latency variability. Second, we quantified the behavior of the algorithm under two distinct signal-to-noise ratio (SNR) conditions: Gaussian noise independently present in each channel, and highly correlated (1/f distributed), far-field noise presented identically in each channel of the array. The mcERP algorithm was found to be robust to noise accurately identifying all component waveshapes and their associated single-trial characteristics down to SNR levels of -20dB for Gaussian noise and -7dB for 1/f far-field noise. Comparisons of the performance of this algorithm with factor analysis (FA) and independent component analysis (ICA) will be described by Knuth et al. (SFN abstracts, 2002). In addition, the advantages of application of mcERP to real data will be described by Shah et al, (these abstracts, 2002: SFN abstracts, 2002).

Knuth, K. H.↗

Molecular genetic analysis of plant gravitropism

The analysis of mutants is a powerful approach for elucidating the components of complex biological processes. A growing number of mutants have been isolated which affect plant gravitropism and the classes of mutants found thus far provide important information about the gravity response mechanism. The wide variety of mutants isolated, especially in Arabidopsis, indicates that gravitropism is a complex, multi-step process. The existence of mutants altered in either root gravitropism alone, shoot gravitropism alone, or both indicates that the root and shoot gravitropic mechanisms have both separate and common steps. Reduced starch mutants have confirmed the role of amyloplasts in sensing the gravity signal. The hormone auxin is thought to act as the transducing signal between the sites of gravity perception (the starch parenchyma cells surrounding the vascular tissue in shoots and the columella cells of root caps) and asymmetric growth (the epidermal cells of the elongation zone(s) of each organ). To date, all mutants that are resistant to high concentrations of auxin have also been found to exhibit a reduced gravitropic response, thus supporting the role of auxin. Not all gravitropic mutants are auxin-resistant, however, indicating that there are additional steps which do not involve auxin. Studies with mutants of tomato which exhibit either reduced or reversed gravitropic responses further support the role of auxin redistribution in gravitropism and suggest that both red light and cytokinin interact with gravitropism through controlling lateral auxin transport. Plant responses to gravity thus likely involve changes in both auxin transport and sensitivity.

Review↗

Springback and diagravitropism in Merit corn roots

Dark-treated Merit corn (Zea mays L.) roots are diagravitropic and lose curvature upon withdrawal of the gravity stimulus (springback). Springback was not detected in a variety of corn that is orthogravitropic in the dark, nor in Merit roots in which tropistic response was enhanced either with red light or with abscisic acid. A possible interpretation is that springback may be associated with a weak growth response of diagravitropic roots.

Non-NASA Center↗

Gene activity during germination of spores of the fern, Onoclea sensibilis: RNA and protein synthesis and the role of stored mRNA

Pattern of 3H-uridine incorporation into RNA of spores of Onoclea sensibilis imbibed in complete darkness (non-germinating conditions) and induced to germinate in red light was followed by oligo-dT cellulose chromatography, gel electrophoresis coupled with fluorography and autoradiography. In dark-imbibed spores, RNA synthesis was initiated about 24 h after sowing, with most of the label accumulating in the high mol. wt. poly(A) -RNA fraction. There was no incorporation of the label into poly(A) +RNA until 48 h after sowing. In contrast, photo-induced spores began to synthesize all fractions of RNA within 12 h after sowing and by 24 h, incorporation of 3H-uridine into RNA of irradiated spores was nearly 70-fold higher than that into dark-imbibed spores. Protein synthesis, as monitored by 3H-arginine incorporation into the acid-insoluble fraction and by autoradiography, was initiated in spores within 1-2 h after sowing under both conditions. Autoradiographic experiments also showed that onset of protein synthesis in the cytoplasm of the germinating spore is independent of the transport of newly synthesized nuclear RNA. One-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis of 35S-methionine-labelled proteins revealed a good correspondence between proteins synthesized in a cell-free translation system directed by poly(A) +RNA of dormant spores and those synthesized in vivo by dark-imbibed and photo-induced spores. These results indicate that stored mRNAs of O. sensibilis spores are functionally competent and provide templates for the synthesis of proteins during dark-imbibition and germination.

NASA Discipline Number 29-20↗

Phytochrome induces changes in the immunodetectable level of a wall peroxidase that precede growth changes in maize seedlings

The regulatory pigment phytochrome induces rapid and opposite growth changes in different regions of etiolated maize seedlings: it stimulates the elongation rate of coleoptiles and inhibits that of mesocotyls. As measured by a quantitative immunoassay, phytochrome also promotes rapid and opposite changes in the extractable content of a Mr 98,000 anionic isoperoxidase in the cell walls of these same organs: it induces a decrease of this peroxidase in coleoptiles and an increase in mesocotyls. The peroxidase changes precede the growth changes. As measured by video stereomicroscopy or a position transducer, red light (R), which photoactivates phytochrome, stimulates coleoptile elongation with a lag of about 15-20 min and suppresses mesocotyl growth with a lag of 45-50 min. R also induces a 50% reduction in the extractable level of the anionic peroxidase in coleoptile walls in less than 10 min and a 40% increase in the level of this peroxidase in mesocotyl walls within 30 min. Ascorbic acid, an inhibitor of peroxidase activity, blocks the effects of R on mesocotyl section growth. These results are relevant to hypotheses that postulate that certain wall peroxidases can participate in light-induced changes in growth rate by their effects on wall extensibility.

Non-NASA Center↗

Polar distribution of annexin-like proteins during phytochrome-mediated initiation and growth of rhizoids in the ferns Dryopteris and Anemia

Although the calcium requirement of phytochrome-mediated fern spore germination and early rhizoid growth is well established, the calcium-binding proteins that serve as transducers for these responses are not known. Here we report the presence of annexin-like proteins in germinating spores of Dryopteris filix-mas (L.) Schott and Anemia phyllitidis (L.) Sw. and evidence that they may be important participants in early photomorphogenic changes in gametophytes. Immunolocalization and immunoblot assays of these proteins were carried out using polyclonal antibodies raised either against a 35-kDa annexin-like protein from pea or against anchorin CII from chicken. Western-blot analysis showed that crude protein extracts obtained from both species after red-light treatment contained two cross-reactive protein bands with molecular weights around 70 kDa. These proteins were annexin-like in that they bound to a phosphatidylserine affinity column in a calcium-dependent fashion. Using this column, two protein bands around 70 kDa, i.e. 67 and 73 kDa, were partially purified together with proteins at 36 kDa and a doublet at 54 kDa. Proteins of these latter molecular weights are suggested to be members of the annexin family, but no cross-reactivity could be found between these and the two antibodies used in our investigations. Immunodetectable levels of these proteins were observed only after light-mediated induction of spore germination. Imaging of the immuno-localization patterns observed with both antibodies showed that the annexin-like proteins are concentrated at the extreme tips of the rhizoids in D. filix-mas and A. phyllitidis during rhizoid initiation and all stages of elongation. We suggest that these proteins may play a major role in the tip-oriented exocytosis events that are critical for the initiation and growth of fern rhizoids.

NASA Discipline Number 40-50↗

Ethylene plays multiple nonprimary roles in modulating the gravitropic response in tomato

Ethylene is known to interact with auxin in regulating stem growth, and yet evidence for the role of ethylene in tropic responses is contradictory. Our analysis of four mutants of tomato (Lycopersicon esculentum) altered in their response to gravity, auxin, and/or ethylene revealed concentration-dependent modulation of shoot gravitropism by ethylene. Ethylene inhibitors reduce wild-type gravicurvature, and extremely low (0.0005-0.001 microliter L-1) ethylene concentrations can restore the reduced gravitropic response of the auxin-resistant dgt (diageotropica) mutant to wild-type levels. Slightly higher concentrations of ethylene inhibit the gravitropic response of all but the ethylene-insensitive nr (never-ripe) mutant. The gravitropic responses of nr and the constitutive-response mutant epi (epinastic) are slightly and significantly delayed, respectively, but otherwise normal. The reversal of shoot gravicurvature by red light in the lz-2 (lazy-2) mutant is not affected by ethylene. Taken together, these data indicate that, although ethylene does not play a primary role in the gravitropic response of tomato, low levels of ethylene are necessary for a full gravitropic response, and moderate levels of the hormone specifically inhibit gravicurvature in a manner different from ethylene inhibition of overall growth.

Non-NASA Center↗

Alterations in endogenous circadian rhythm of core temperature in senescent Fischer 344 rats

We assessed whether alterations in endogenous circadian rhythm of core temperature (CRT) in aging rats are associated with chronological time or with a biological marker of senescence, i.e., spontaneous rapid body weight loss. CRT was measured in male Fischer 344 (F344) rats beginning at age 689 days and then continuously until death. Young rats were also monitored. The rats were housed under constant dim red light at 24-26 degrees C, and core temperature was recorded every 10 min via biotelemetry. The CRT amplitude of the body weight-stable (presenescent) old rats was significantly less than that of young rats at all analysis periods. At the onset of spontaneous rapid weight loss (senescence), all measures of endogenous CRT differed significantly from those in the presenescent period. The suprachiasmatic nucleus (a circadian pacemaker) of the senescent rats maintained its light responsiveness as determined by an increase in c-fos expression after a brief light exposure. These data demonstrate that some characteristics of the CRT are altered slowly with chronological aging, whereas others occur rapidly with the onset of senescence.

Non-NASA Center↗

The HST/STIS Next Generation Spectral Library

During Cycles 10, 12, and 13, we obtained STIS G230LB, G430L, and G750L spectra of 378 bright stars covering a wide range in abundance, effective temperature, and luminosity. This HST/STIS Next Generation Spectral Library was scheduled to reach its goal of 600 targets by the end of Cycle 13 when STIS came to an untimely end. Even at 2/3 complete, the library significantly improves the sampling of stellar atmosphere parameter space compared to most other spectral libraries by including the near-UV and significant numbers of metal poor and super-solar abundance stars. Numerous calibration challenges have been encountered, some expected, some not; these arise from the use of the E1 aperture location, non-standard wavelength calibration, and, most significantly, the serious contamination of the near-UV spectra by red light. Maximizing the utility of the library depends directly on overcoming or at least minimizing these problems, especially correcting the UV spectra.

Gregg, M. D.↗

Two-Finger EKG Method of Detecting Evasive Responses

A system based on acquisition and processing of electrocardiographic (EKG) signals from two fingers has been proposed as a means of determining whether a person is answering questions evasively. The system -- in effect, a "lie detector" of sorts, would be used to gauge prospective passengers responses to questions at airport security checkpoints. The person to be interrogated would be required to put one finger from each hand onto two metal strips on a counter and would be instructed to relax and wait for the system to indicate that it is ready. Then a security person would ask the passenger a set of standard security questions. EKG signals acquired through contact with the fingers would be processed by an algorithm that would extract a single-value measure of the level of stress in the interrogated person. This measure would be used to determine whether the system should display a green or a red light to signify that the person is or is not, respectively, telling the truth. It has also been conjectured that the system may be useful for communicating with a person who is in a coma and, hence, unable to speak.

Oldham, Daniel↗

Species Specific Bacterial Spore Detection Using Lateral-Flow Immunoassay with DPA-Triggered Tb Luminescence

A method of detecting bacterial spores incorporates (1) A method of lateral-flow immunoassay in combination with (2) A method based on the luminescence of Tb3+ ions to which molecules of dipicolinic acid (DPA) released from the spores have become bound. The present combination of lateral-flow immunoassay and DPA-triggered Tb luminescence was developed as a superior alternative to a prior lateral-flow immunoassay method in which detection involves the visual observation and/or measurement of red light scattered from colloidal gold nanoparticles. The advantage of the present combination method is that it affords both (1) High selectivity for spores of the species of bacteria that one seeks to detect (a characteristic of lateral-flow immunoassay in general) and (2) Detection sensitivity much greater (by virtue of the use of DPA-triggered Tb luminescence instead of gold nanoparticles) than that of the prior lateral-flow immunoassay method

Ponce, Adrian↗

Efficiency of Photosynthesis in a Chl d-Utilizing Cyanobacterium is Comparable to or Higher than that in Chl a-Utilizing Oxygenic Species

The cyanobacterium Acaryochloris marina uses chlorophyll d to carry out oxygenic photosynthesis in environments depleted in visible and enhanced in lower-energy, far-red light. However, the extent to which low photon energies limit the efficiency of oxygenic photochemistry in A. marina is not known. Here, we report the first direct measurements of the energy-storage efficiency of the photosynthetic light reactions in A. marina whole cells,and find it is comparable to or higher than that in typical, chlorophyll a-utilizing oxygenic species. This finding indicates that oxygenic photosynthesis is not fundamentally limited at the photon energies employed by A. marina, and therefore is potentially viable in even longer-wavelength light environments.

Mielke, S. P.↗

Characterization of Firefly, an Imaging Spectrometer Designed for Airborne Measurements of Solar-Induced Fluorescence

FIREFLY (Fluorescence Imaging of REd and Far-red Light Yield) is a compact, fine-resolution imaging spectrometer that was designed and assembled by Headwall Photonics (Fitchburg, MA, USA) in collaboration with NASA scientists for airborne measurements of Solar-Induced Fluorescence (SIF). FIREFLY is integrated into the next generation of NASA Goddard’s Lidar, Hyperspectral and Thermal airborne imager (G-LiHT; www.gliht.nasa.gov), providing a complete system for measuring, interpreting and scaling SIF emissions. Characterization of FIREFLY was performed here to evaluate its performance and suitability for retrieving SIF.

Imaging Spectroscopy↗