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At least 109 records · Page 6

Diverse Profile of Fermentation Byproducts From Thin Stillage

The economy of biorefineries is influenced not only by biofuel production from carbohydrates but also by the production of valuable compounds from largely underutilized industrial residues. Currently, the demand for many chemicals that could be made in a biorefinery, such as succinic acid (SA), medium-chain fatty acids (MCFAs), and lactic acid (LA), is fulfilled using petroleum, palm oil, or pure carbohydrates as raw materials, respectively. Thin stillage (TS), the residual liquid material following distillation of ethanol, is an underutilized coproduct from the starch biofuel industry. This carbon-rich material has the potential for chemical upgrading by microorganisms. Here, we explored the formation of different fermentation products by microbial communities grown on TS using different bioreactor conditions. At the baseline operational condition (6-day retention time, pH 5.5, 35°C), we observed a mixture of MCFAs as the principal fermentation products. Operation of a bioreactor with a 1-day retention time induced an increase in SA production, and a temperature increase to 55°C resulted in the accumulation of lactic and propionic acids. In addition, a reactor operated with a 1-day retention time at 55°C conditions resulted in LA accumulation as the main fermentation product. The prominent members of the microbial community in each reactor were assessed by 16S rRNA gene amplicon sequencing and phylogenetic analysis. Under all operating conditions, members of the Lactobacillaceae family within Firmicutes and the Acetobacteraceae family within Proteobacteria were ubiquitous. Members of the Prevotellaceae family within Bacteroidetes and Lachnospiraceae family within the Clostridiales order of Firmicutes were mostly abundant at 35°C and not abundant in the microbial communities of the TS reactors incubated at 55°C. The ability to adjust bioreactor operating conditions to select for microbial communities with different fermentation product profiles offers new strategies to explore and compare potentially valuable fermentation products from TS and allows industries the flexibility to adapt and switch chemical production based on market prices and demands.

59 BASIC BIOLOGICAL SCIENCES↗

Understanding the Drivers of Mobility during the COVID-19 Pandemic in Florida, USA Using a Machine Learning Approach

As of March 2021, the State of Florida, U.S.A. had accounted for approximately 6.67% of total COVID-19 (SARS-CoV-2 coronavirus disease) cases in the U.S. The main objective of this research is to analyze mobility patterns during a three month period in summer 2020, when COVID-19 case numbers were very high for three Florida counties, Miami-Dade, Broward, and Palm Beach counties. To investigate patterns, as well as drivers, related to changes in mobility across the tri-county region, a random forest regression model was built using sociodemographic, travel, and built environment factors, as well as COVID-19 positive case data. Mobility patterns declined in each county when new COVID-19 infections began to rise, beginning in mid-June 2020. While the mean number of bar and restaurant visits was lower overall due to closures, analysis showed that these visits remained a top factor that impacted mobility for all three counties, even with a rise in cases. Our modeling results suggest that there were mobility pattern differences between counties with respect to factors relating, for example, to race and ethnicity (different population groups factored differently in each county), as well as social distancing or travel-related factors (e.g., staying at home behaviors) over the two time periods prior to and after the spike of COVID-19 cases.

60 APPLIED LIFE SCIENCES↗

Field intercomparison of ice nucleation measurements: the Fifth International Workshop on Ice Nucleation Phase 3 (FIN-03)

Abstract. The third phase of the Fifth International Ice Nucleation Workshop (FIN-03) was conducted at the Storm Peak Laboratory in Steamboat Springs, Colorado, in September 2015 to facilitate the intercomparison of instruments measuring ice-nucleating particles (INPs) in the field. Instruments included two online and four offline measurement systems for INPs, which are a subset of those utilized in the laboratory study that comprised the second phase of FIN (FIN-02). The composition of the total aerosols was characterized using the Particle Analysis by Laser Mass Spectrometry (PALMS) and Wideband Integrated Bioaerosol Sensor (WIBS) instruments, and aerosol size distributions were measured by a laser aerosol spectrometer (LAS). The dominant total particle compositions present during FIN-03 were composed of sulfates, organic compounds, and nitrates, as well as particles derived from biomass burning. Mineral-dust-containing particles were ubiquitous throughout and represented 67 % of supermicron particles. Total WIBS fluorescing particle concentrations for particles with diameters of > 0.5 µm were 0.04 ± 0.02 cm−3 (0.1 cm−3 highest; 0.02 cm−3 lowest), typical of the warm season in this region and representing ≈ 9 % of all particles in this size range as a campaign average. The primary focus of FIN-03 was the measurement of INP concentrations via immersion freezing at temperatures > −33 °C. Additionally, some measurements were made in the deposition nucleation regime at these same temperatures, representing one of the first efforts to include both mechanisms within a field campaign. INP concentrations via immersion freezing agreed within factors ranging from nearly 1 to 5 times on average between matched (time and temperature) measurements, and disagreements only rarely exceeded 1 order of magnitude for sampling times coordinated to within 3 h. Comparisons were restricted to temperatures lower than −15 °C due to the limits of detection related to sample volumes and very low INP concentrations. Outliers of up to 2 orders of magnitude occurred between −25 and −18 °C; a better agreement was seen at higher and lower temperatures. Although the 5–10 factor agreement of INP measurements found in FIN-03 aligned with the results of the FIN-02 laboratory comparison phase, giving confidence in progress of this measurement field, this level of agreement still equates to temperature uncertainties of 3.5 to 5 °C that may not be sufficient for numerical cloud modeling applications that utilize INP information. INP activity in the immersion-freezing mode was generally found to be an order of magnitude or more, making it more efficient than in the deposition regime at 95 %–99 % water relative humidity, although this limited data set should be augmented in future efforts. To contextualize the study results, an assessment was made of the composition of INPs during the late-summer to early-fall period of this study inferred through comparison to existing ice nucleation parameterizations and through measurement of the influence of thermal and organic carbon digestion treatments on immersion-freezing ice nucleation activity. Consistent with other studies in continental regions, biological INPs dominated at temperatures of > −20 °C and sometimes colder, while arable dust-like or other organic-influenced INPs were inferred to dominate below −20 °C.

54 ENVIRONMENTAL SCIENCES↗

Ni Mediated Carboxylation and Oxidation of PE to C12/C14 Fatty Acids: Cooperative Research and Development Final Report, CRADA Number CRD-22-22451

Given its chemical structure, polyethylene (PE) waste may be a viable feedstock for the production of linear fatty alcohols (FALCs), a major surfactant produced either from palm kernel oil, vegetable oils, or via synthetic pathways that are associated with high carbon footprint. The BOTTLE Consortium will collaborate with Procter & Gamble (P&G) to develop FALCs from waste PE feedstocks.

09 BIOMASS FUELS↗

Mercury Exposure is Associated with Urbanization but not Salmonella Status in White Ibis ( Eudocimus albus )

Mercury (Hg) is a ubiquitous contaminant in wetlands that can cause immunosuppression in birds, which may increase susceptibility to colonization with Salmonella spp. Previously, we found that White Ibis (Eudocimus albus), a recently urbanized wading bird, shed Salmonella spp. at a higher prevalence when captured at urban sites, compared with natural sites. In this study, we sought to determine if Hg burdens in ibis are related to Salmonella status or degree of urbanization or both. We analyzed feathers from 94 ibis in Palm Beach County, Florida, USA, along an urbanization gradient (0–68% urbanization) and from individuals with confirmed Salmonella spp. status (shedding or not shedding). We detected Hg in all ibis feathers (0.22–8.47 mg/kg; mean=1.96 mg/kg; SD=1.94). The Hg concentration was not significantly correlated to Salmonella spp. shedding status (Wilcoxon rank sum test, W=1170; P=0.596) but was negatively associated with capture site urbanization level (R 2 =0.327; P=0.026). Our findings may suggest that the immunosuppressive effects of Hg do not affect Salmonella shedding in the ibis or that Hg burdens were too low to affect Salmonella shedding status. Further, ibis that were captured in high urbanization sites appeared to have a lower risk of Hg exposure than ibis that were captured within low urbanization sites.

59 BASIC BIOLOGICAL SCIENCES↗

Therapeutic hand-exercising device with cycling pressure value

Device exercises hands of persons whose fingers are generally straight and need to be flexed inward toward palms of hands. Device is extremely simple in design, which reduces costs, and fits all hand sizes. Patient can instantly free hand from device by pulling flap free from wrist of straps.

Barthlome, D. E.↗

The application of Skylab imagery to analysis of fault tectonics and earthquake hazards in the Peninsular Ranges, southern California

The author has identified the following significant results. Frame 114 of the Salton Sea area was studied in all bands to analyze the appearance of important faults. These faults were also studied in the field as well as from aircraft and in aerial photography. The San Andreas/Banning and the Mission Creek faults can be traced across Coachella Valley even though they are buried by alluvium. The faults form ground water barriers and the near surface ground water on the northeast sides of the faults supports patches of vegetation (mesquite and palms) in an otherwise barren desert. These oases are best seen in band 3 (color IR). Otherwise, faults are best seen in band 4 (aerial color). Of the B and W bands, 5 (red) is best for delineating faults. Bands 1 and 2 are excessively grainy and the resolution is considerably inferior to the other bands.

Merifield, P. M.↗

Metering gun for dispensing precisely measured charges of fluid

A cyclically operable fluid dispenser for use in dispensing precisely measured charges of potable water aboard spacecraft is described. The dispenser is characterized by (1) a sealed housing adapted to be held within a crewman's palm and coupled with a pressurized source of potable water; (2) a dispensing jet projected from the housing and configured to be received within a crewman's lips; (3) an expansible measuring chamber for measuring charges of drinking water received from the source; (4) and a dispenser actuator including a lever extended from the housing to be digitated for initiating operational cycles, whereby precisely measured charges of potable water selectively are delivered for drinking purposes in a weightless environment.

Cook, T. A.↗

Manual actuator

An actuator for an exercising machine employable by a crewman aboard a manned spacecraft is presented. The actuator is characterized by a force delivery arm projected from a rotary imput shaft of an exercising machine and having a force input handle extended orthogonally from its distal end. The handle includes a hand-grip configured to be received within the palm of the crewman's hand and a grid pivotally supported for angular displacement between a first position, wherein the grid is disposed in an overlying juxtaposition with the hand-grip, and a second position, angularly displaced from the first position, for affording access to the hand-grip, and a latching mechanism fixed to the sole of a shoe worn by the crewman for latching the shoe to the grid when the grid is in the first position.

Gause, R. L.↗

Design and fabrication of an end effector

The construction is described of a prototype mechanical hand or 'end effector' for use on a remotely controlled robot, but with possible application as a prosthetic device. An analysis of hand motions is reported, from which it is concluded that the two most important manipulations (apart from grasps) are to be able to pick up a tool and draw it into a nested grip against the palm, and to be able to hold a pistol-grip tool such as an electric drill and pull the trigger. A model was tested and found capable of both these operations.

Crossley, F. R. E.↗

Design for a three-fingered hand

This paper describes the construction of a prototype mechanical hand or 'end effector' for use on a remotely controlled robot, but with possible application as a prosthetic device. An analysis of hand motions is reported, from which it is concluded that the two most important manipulations (apart from grasps) are to be able to pick up a tool and draw it into a nested grip against the palm, and to be able to hold a pistol-grip tool such as an electric drill and pull the trigger. One of our models was tested and found capable of both these operations.

Crossley, F. R. E.↗

Compact ratchet wrench

Round ratchet wrench handle that fits into palm can be inserted into most areas that are inaccessible to wrenches with conventional handles.

Stringer, E. J.↗

Restraining mechanism

A restraining mechanism restraining a pressurized garment so as to limit its ballooning effect is described. A helically wound spring is bonded at its outer periphery to an elongated flat plate which permits the flat plate to bend in a single direction. The flat plate is attached to an inflatable glove to the palm side for restraining the glove from ballooning when inflated.

Hardy, J. C.↗

The effect of nonuniform wind shear on the intensification and reflection of mountain waves

A study is made of the intensification and reflection of mountain waves when the shear of the basic wind profile is nonuniform. A two-layer atmospheric model is treated, and the wind profile in the troposphere is assumed to be parabolic. The Scorer parameter includes the wind profile curvature term, which may not be neglected if the Richardson number Ri is finite. When Ri is finite, the optimal phase difference across the troposphere for maximum surface velocity intensifications is found to be slightly greater than Pi. As Ri increases, the optimal phase difference decreases with Ri and approaches the limiting value Pi. This implies that waves approximately reverse phase between the surface and the tropopause for maximum wave intensifications in most physically realistic atmospheric situations. The concept of Eliassen and Palm concerning the additivity of the vertical wave energy fluxes is expanded (valid at least up to the parabolic wind profile), by which the upward and downward energy transporting modes are identified.

Hyun, J. M.↗

Thermally Insulated Glove With Good Tactility

Thermally insulated glove contains short, closely-spaced elastomeric pins that insulate without impairing flexibility. By confining pins to the inter-joint areas of palm, fingers and back of the hand, joint mobility is retained. Glove thermal-insulation requirements dictate the relationships among pin length, pin diameter, and number of pins per unit surface length.

Balinskas, R.↗

Some Eulerian and Lagrangian diagnostics for a model stratospheric warming

The Eliassen-Palm (EP) cross sections for the model are discussed. EP cross sections conveniently display the principal eddy fluxes of heat, momentum, and quasi-geostrophic potential vorticity on one diagram. The EP diagnostics are used to follow the evolution of the mean flow through into the second stage of the model warming, which includes the reversal of the polar westerlies and which appears to have more in common with observed warmings. The transformed Eulerian-mean equations presented by Andrews and McIntyre (1976, 1978) provide the basis for an investigation in which an especially clear picture of the warming dynamics is obtained. In the second stage of the model warming, the decelerating torque per unit mass is large throughout a deep layer in the middle atmosphere, spanning several scale heights.

Dunkerton, T.↗

Nonslip Wristlet

Simple garmet protects vulnerable area between sleeve end and glove. Mittenlike wristlet is restrained by thumb loop and cuff attachment with either single finger opening or separate openings for each digit. Worn under glove, wristlet provides additional layer of protective material over palm, back of hand, and over web of thumb.

Himel, V.↗