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94 records · Page 6

Elevated acetate concentrations in the rhizosphere of Spartina alterniflora and potential influences on sulfate reduction

Acetate is important in anaerobic metabolism of non-vegetated sediments but its role in salt marsh soils was not investigated thoroughly. Acetate concentrations, oxidation (C-14) and SO4(2-) reduction (S-35) were measured in S. alterniflora soils in NH and MA. Pore water from cores contained greater than 0.1 mM acetate and in some instances greater than 1.0 mM. Non-destructive samples contained less than 0.01 mM. Acetate was associated with roots and concentrations were highest during vegetative growth and varied with changes in plant physiology. Acetate turnover was very low whether whole core or slurry incubations were used. Radiotracers injected directly into soils yielded rates of SO4(2-) reduction and acetate oxidation not significantly different from core incubation techniques. Regardless of incubation method, acetate oxidation did not account for a significant percentage of SO4(2-) reduction. These results differ markedly from data for non-vegetated coastal sediments where acetate levels are low, oxidation rate constants are high and acetate oxidation rates greatly exceed rates of SO4(2-) reduction. The discrepancy between rates of acetate oxidation and SO4(2-) reduction in marsh soils may be due either to the utilization of substrates other than acetate by SO4(2-) reducers or artifacts associated with measurements of organic utilization by rhizosphere bacteria.

Hines, Mark E.↗

Role of Boreal Vegetation in Controlling Ecosystem Processes and Feedbacks to Climate

In the field, dark respiration rates are greatest in cores from more northerly locations. This is due in part to greater amounts of dwarf shrub biomass in the more northerly cores, but also to differences in soil organic matter quality. Laboratory incubations of these soils under common conditions show some evidence for greater pools of available carbon in soils from more northerly tundra sites, although the most northerly site does not fit this pattern for reasons which are unclear at this time. While field measurements of cores transplanted among different vegetation types at the same location (Toolik Lake) show relatively small differences in whole ecosystem carbon flux, laboratory incubation of these same soils shows that there are large differences in soil respiration rates under common conditions. This is presumably due to differences in organic matter quality. Microenvironmental site factors (temperature, soil moisture, degree of anaerobiosis, etc.) may be responsible for evening out these differences in the field. These site factors, which differ with slope, aspect, and drainage within a given location along the latitudinal gradient, appear to exert at least as strong a control over carbon fluxes as do macroclimatic factors among sites across the latitudinal gradient. While our field measurements indicate that, in the short term, warming will tend to increase ecosystem losses Of CO2 via respiration more than they will increase plant gross assimilation, the degree to which different topographically-defined plant communities will respond is likely to vary.

Chapin, F. S., III↗

Reduced naphthylphthalamic acid binding in the tir3 mutant of Arabidopsis is associated with a reduction in polar auxin transport and diverse morphological defects

Polar auxin transport plays a key role in the regulation of plant growth and development. To identify genes involved in this process, we have developed a genetic procedure to screen for mutants of Arabidopsis that are altered in their response to auxin transport inhibitors. We recovered a total of 16 independent mutants that defined seven genes, called TRANSPORT INHIBITOR RESPONSE (TIR) genes. Recessive mutations in one of these genes, TIR3, result in altered responses to transport inhibitors, a reduction in polar auxin transport, and a variety of morphological defects that can be ascribed to changes in indole-3-acetic acid distribution. Most dramatically, tir3 seedlings are strongly deficient in lateral root production, a process that is known to depend on polar auxin transport from the shoot into the root. In addition, tir3 plants display a reduction in apical dominance as well as decreased elongation of siliques, pedicels, roots, and the inflorescence. Biochemical studies indicate that tir3 plants have a reduced number of N-1-naphthylphthalamic (NPA) binding sites, suggesting that the TIR3 gene is required for expression, localization, or stabilization of the NPA binding protein (NBP). Alternatively, the TIR3 gene may encode the NBP. Because the tir3 mutants have a substantial defect in NPA binding, their phenotype provides genetic evidence for a role for the NBP in plant growth and development.

Non-NASA Center↗

Systems and methods for laser diode array having integrated microchannel cooling

The present disclosure relates to a laser diode system. The system may have at least one laser diode emitter having a substrate, at least one laser diode supported on the substrate, and a facet which a laser beam generated by the laser diode is emitted. A cooling subsystem is included which is disposed in contact with the substrate of the laser diode emitter. The cooling subsystem includes a plurality of cooling fins forming a plurality of elongated channels for circulating a cooling fluid therethrough to cool the laser diode emitter. The cooling fluid also flows over the facet of the laser diode emitter.

Kotovsky, Jack↗