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At least 109 records · Page 6

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DOE OSTI2025

ModE Regulates Alternative Nitrogenase Expression in the Methanogen Methanosarcina acetivorans

ABSTRACT All methanogens that can fix nitrogen use molybdenum (Mo) nitrogenase. Some methanogens, including Methanosarcina acetivorans , also contain alternative vanadium‐ and iron‐nitrogenases, encoded by the vnf and anf operons, respectively. These nitrogenases are produced when there is insufficient Mo to support Mo‐nitrogenase activity. The factors that control the expression of the alternative nitrogenases in response to Mo availability are unknown in methanogens. Here we show that ModE is the regulator that represses transcription of the vnf and anf operons in M. acetivorans when cells are grown with Mo. CRISPRi repression of modE results in a significant increase in the transcription of the vnf and anf operons as well as the detection of Fe‐nitrogenase during nitrogen fixation in the presence of Mo. Gel shift assays with recombinant ModE demonstrated that ModE binds a specific sequence motif upstream of the vnf and anf operons, as well as other genes and operons related to nitrogen fixation and Mo transport. However, purified ModE does not contain Mo, and the addition of Mo does not alter the affinity of ModE for DNA, indicating M. acetivorans ModE may not directly bind Mo. This study shows that ModE is the primary Mo‐responsive regulator of alternative nitrogenase expression in M. acetivorans , but other factor(s) are likely involved in directly sensing Mo.

Chanderban, Melissa [Department of Biological Scie↗

DOE OSTI2025

Center for Alternate Synchronization and Timing (CAST) PTP Network Monitoring Report

The Oak Ridge National Laboratory Center for Alternative Synchronization and Timing (CAST) performs research, development, testing, and evaluation of alternative terrestrial-based timing and synchronization infrastructure for the US power grid and other critical infrastructures. Alternative timing options reduce reliance on GPS and enhance the overall resilience of critical infrastructures. CAST infrastructure uses Precision Time Protocol (PTP) as the primary conduit for delivery of synchronization packets. CAST deploys PTP over long terrestrial links to synchronize a multitude of remote boundary clocks and downstream power grid components with the authoritative grand master clocks. Network traffic issues can severely degrade PTP accuracy. This report focuses on examining network traffic anomalies and their effects on PTP operation as well as the potential implications to CAST’s high-precision remote synchronization operations.

24 POWER TRANSMISSION AND DISTRIBUTION↗

NASA NTRS1972

The elastic analysis of the part-circular surface flaw problem by the alternating method.

This paper summarizes and evaluates the work done on the elastic analysis of the surface flaw problem by the application of the alternating method. An attempt is made to describe the alternating method and to present the history of its application to the surface flaw problem and to related problems in fracture mechanics. Stress intensity factors obtained by this method are summarized and compared. Results are also compared to those obtained by investigators using other methods of analysis. An evaluation of the use of the alternating method is presented with the purpose of pointing out the advantages and disadvantages in the application of this technique to the surface flaw problem.

Smith, F. W.↗

NASA NTRS1972

Rotor windage losses for Lundell alternators.

A range of experimental data was generated to permit accurate calculation of the windage power loss for Lundell alternator design. Windage tests were conducted on two concentric rotor-stator configurations in ambient air, producing Reynolds numbers as high as 100,000. Cylindrical rotors 10 and 12 inches in diameter were operated up to 24,000 rpm, and an 8 inch diameter Lundell rotor was operated up to 36,000 rpm. Gap-to-radius ratios of 0.01, 0.02 and 0.04 were tested. Tests were also conducted with axial slots machined on the stator surfaces to simulate alternator winding slots. Using the data obtained, a method was developed to calculate the windage loss for any Lundell alternator given the geometry and cavity conditions.

Gorland, S. H.↗

NASA NTRS1972

Parallel operation of two Brayton-cycle alternators with parasitic speed controllers.

The experimental paralleling characteristics of two 1200-Hz Brayton-cycle alternators are presented. Since the Brayton power conversion system uses electric speed controllers, the paralleling requirements are somewhat different from those for conventional ground-based power systems. Results include the transient effects of synchronizing the two alternators with various phase-angle, voltage, and frequency differences. Based on these results, the effects of synchronizing differences can be defined, and adjustment requirements of the parasitic speed controllers during synchronizing can be established. Data indicate that parasitically loaded alternators are able to parallel over a wide range of synchronizing differences.

Perz, D. A.↗

NASA NTRS1973

Brayton heat exchanger unit development program (alternate design)

A Brayton Heat Exchanger Unit Alternate Design (BHXU-Alternate) consisting of a recuperator, a heat sink heat exchanger, and a gas ducting system, was designed and fabricated. The design was formulated to provide a high performance unit suitable for use in a long-life Brayton-cycle powerplant. Emphasis was on double containment against external leakage and leakage of the organic coolant into the gas stream. A parametric analysis and design study was performed to establish the optimum component configurations to achieve low weight and size and high reliability, while meeting the requirements of high effectiveness and low pressure drop. Layout studies and detailed mechanical and structural design were performed to obtain a flight-type packaging arrangement, including the close-coupled integration of the BHXU-Alternate with the Brayton Rotating Unit (BRU).

Duncan, J. D.↗

NASA NTRS1974

Determination of life for a polyimide-epoxy alternator insulation system

Tests were conducted to predict remaining electrical insulation life of a polyimide epoxy insulated 60 KW, 208 volt homopolar inductor alternator, following completion of 23,130 hours of turbo-alternator endurance tests. The sectioned armature winding of this alternator stator was used as means to evaluate and measure end-life at several aging temperatures for development of an Arrhenius plot. A one-half life rate of 11.3 C was established from these data with a predicted remaining life of 60,000 hours at an armature winding temperature of 248 C and a total life, including endurance test time, of 61,645 hours.

Penn, W. B.↗

NASA NTRS1976

Transient analysis of unbalanced short circuits of the ERDA-NASA 100 kW wind turbine alternator

Unbalanced short-circuit faults on the alternator of the ERDA-NASA Mod-O100-kW experimental wind turbine are studied. For each case, complete solutions for armature, field, and damper-circuit currents; short-circuit torque; and open-phase voltage are derived directly by a mathematical analysis. Formulated results are tabulated. For the Mod-O wind turbine alternator, numerical calculations are given, and results are presented by graphs. Comparisons for significant points among the more important cases are summarized. For these cases the transients are found to be potentially severe. The effect of the alternator neutral-to-ground impedance is evaluated.

Hwang, H. H.↗

NASA NTRS1976

Air transportation energy efficiency - Alternatives and implications

Results from recent studies of air transportation energy efficiency alternatives are discussed, along with some of the implications of these alternatives. The fuel-saving alternatives considered include aircraft operation, aircraft modification, derivative aircraft, and new aircraft. In the near-term, energy efficiency improvements should be possible through small improvements in fuel-saving flight procedures, higher density seating, and higher load factors. Additional small near-term improvements could be obtained through aircraft modifications, such as the relatively inexpensive drag reduction modifications. Derivatives of existing aircraft could meet the requirements for new aircraft and provide energy improvements until advanced technology is available to justify the cost of a completely new design. In order to obtain significant improvements in energy efficiency, new aircraft must truly exploit advanced technology in such areas as aerodynamics, composite structures, active controls, and advanced propulsion.

Williams, L. J.↗

NASA NTRS1978

An alternating direction implicit method for the Control Data STAR-100 vector computer

An implementation of the alternating direction implicit (ADI) method for the Control Data STAR-100 computer is presented and analyzed. Two parallel algorithms, both of which are most efficient when used to solve many independent tridiagonal systems of equations, are discussed relative to their usefulness in an ADI implementation on the STAR-100 computer. It is shown that it may be desirable to alternate between the parallel algorithms as the direction of implicitness is alternated in order to eliminate the data rearrangement which would otherwise be required. The applicability of the two parallel tridiagonal solvers to several other numerical algorithms is also discussed.

Lambiotte, J. J., Jr.↗

NASA NTRS1980

Selection of alternative central-station technologies for the Satellite Power System (SPS) comparative assessment

An important effort is the Satellite Power System (SPS) comparative Assessment is the selection and characterization of alternative technologies to be compared with the SPS concept. The ground rules, criteria, and screening procedure applied in the selection of those alternative technologies are summarized. The final set of central station alternatives selected for comparison with the SPS concept includes: (1) light water reactor with improved fuel utilization, (2) conventional coal combustion with improved environmental controls, (3) open cycle gas turbine with integral low Btu gasifier, (4) terrestrial photovoltaic, (5) liquid metal fast breeder reactor, and (6) magnetic confinement fusion.

Samsa, M.↗

NASA NTRS1980

Alternate symbol inversion for improved symbol synchronization in convolutionally coded systems

Inverting alternate symbols of the encoder output of a convolutionally coded system provides sufficient density of symbol transitions to guarantee adequate symbol synchronizer performance, a guarantee otherwise lacking. Although alternate symbol inversion may increase or decrease the average transition density, depending on the data source model, it produces a maximum number of contiguous symbols without transition for a particular class of convolutional codes, independent of the data source model. Further, this maximum is sufficiently small to guarantee acceptable symbol synchronizer performance for typical applications. Subsequent inversion of alternate detected symbols permits proper decoding.

Simon, M. K.↗

NASA NTRS1981

Compatibility of alternative fuels with advanced automotive gas turbine and stirling engines. A literature survey

The application of alternative fuels in advanced automotive gas turbine and Stirling engines is discussed on the basis of a literature survey. These alternative engines are briefly described, and the aspects that will influence fuel selection are identified. Fuel properties and combustion properties are discussed, with consideration given to advanced materials and components. Alternative fuels from petroleum, coal, oil shale, alcohol, and hydrogen are discussed, and some background is given about the origin and production of these fuels. Fuel requirements for automotive gas turbine and Stirling engines are developed, and the need for certain reseach efforts is discussed. Future research efforts planned at Lewis are described.

Cairelli, J.↗

NASA NTRS1981

Assessment of a satellite power system and six alternative technologies

The satellite power system is assessed in comparison to six alternative technologies. The alternatives are: central-station terrestrial photovoltaic systems, conventional coal-fired power plants, coal-gasification/combined-cycle power plants, light water reactor power plants, liquid-metal fast-breeder reactors, and fusion. The comparison is made regarding issues of cost and performance, health and safety, environmental effects, resources, socio-economic factors, and institutional issues. The criteria for selecting the issues and the alternative technologies are given, and the methodology of the comparison is discussed. Brief descriptions of each of the technologies considered are included.

Wolsko, T.↗

NASA NTRS1982

An Assessment of Alternate Thermal Protection Systems for the Space Shuttle Orbiter: Executive Summary - Volume 1

Alternate thermal protection system (TPS) concepts to the Space Shuttle Orbiter were assessed. Metallic, ablator, and carbon-carbon concepts which are the result of some previous design, manufacturing and testing effort were considered. Emphasis was placed on improved TPS durability, which could potentially reduce life cycle costs and improve Orbiter operational characteristics. Integrated concept/orbiter point designs were generated and analyzed on the basis of Shuttle design environments and criteria. A merit function evaluation methodology based on mission impact, life cycle costs, and risk was developed to compare the candidate concepts and to identify the best alternate. Voids and deficiencies in the technology were identified, along with recommended activities to overcome them. Finally, programmatic plans, including ROM costs and schedules, were developed for all activities required to bring the selected alternate system up to operational readiness.

Hays, D.↗

NASA NTRS1982

Assessment of alternate thermal protection systems for the Space Shuttle Orbiter

Technical aspects of the alternate thermal protection system (TPS) study for the Shuttle Orbiter are reviewed, and a status report on alternate TPS technology developments is presented. Mission impact, life cycle costs and risks, and selected candidate concepts are identified. The best system would consist of mechanically attached metallic and carbon-carbon TPS concepts employing a titanium multiwall prepackaged concept at temperatures below 1000 F, a superalloy honeycomb prepackaged concept at temperatures between 1000-1800 F, and an advanced carbon-carbon multipost standoff concept above 1800 F. Alternative concepts offer significant improvements in durability and are mass competitive with current ceramic tile reusable surface insulation.

Kelly, H. N.↗

NASA NTRS1981

Assessment of alternative power sources for mobile mining machinery

Alternative mobile power sources for mining applications were assessed. A wide variety of heat engines and energy systems was examined as potential alternatives to presently used power systems. The present mobile power systems are electrical trailing cable, electrical battery, and diesel - with diesel being largely limited in the United States to noncoal mines. Each candidate power source was evaluated for the following requirements: (1) ability to achieve the duty cycle; (2) ability to meet Government regulations; (3) availability (production readiness); (4) market availability; and (5) packaging capability. Screening reduced the list of candidates to the following power sources: diesel, stirling, gas turbine, rankine (steam), advanced electric (batteries), mechanical energy storage (flywheel), and use of hydrogen evolved from metal hydrides. This list of candidates is divided into two classes of alternative power sources for mining applications, heat engines and energy storage systems.

Cairelli, J. E.↗

NASA NTRS1989

Alternating-Gradient Photodetector For Far Infrared

Proposed detector of photons of wavelengths in range of 30 to 200 micrometer made of alternating layers of lightly and heavily negatively doped germanium. Formed in sequence by conventional chemical-vapor deposition. Alternating-gradient structure enhances collection of photogenerated charge carriers while suppressing dark current, thus achieving high detectivity. Alternating layers of n+ and n- germanium provides high detectivity in far-infrared spectral region. Also possible to make similar structures with positive doping and with other semiconductors as silicon or gallium arsenide to obtain various spectral response.

Overhauser, Albert W.↗