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At least 109 records · Page 6

Remote sensing of seasonal variation of LAI and fAPAR in a deciduous broadleaf forest

Climate change is affecting the phenology of terrestrial ecosystems. In deciduous forests, phenology in leaf area index (LAI) is the primary driver of seasonal variation in the fraction of absorbed photosynthetically active radiation (fAPAR), which drives photosynthesis. Remote sensing has been widely used to estimate LAI and fAPAR. However, while many studies have examined both empirical and model-based relationships among LAI, fAPAR, and spectral vegetation indices (SVI) from remote sensing, few studies have systematically and empirically examined how relationships among these variables change over the growing season. In this study, we examine how and why seasonal-scale covariation differs among time series of remotely sensed SVIs and both LAI and fAPAR based on current understanding and theory. To do this we use newly available remote sensing data sets in combination with time series of in-situ measurements and a canopy radiative transfer model to analyze how seasonal variation in canopy and environmental conditions affect relationships among remotely sensed SVIs, LAI, and fAPAR at a temperate deciduous forest site in central Massachusetts. Our results show that accounting for seasonal variation in canopy shadowing, which is driven by variation in solar zenith angle, improved remote sensing-based estimates of LAI, fAPAR, and daily total APAR. Specifically, we show that the phenology of SVIs is strongly influenced by seasonal variation in near infrared (NIR) reflectance arising from systematic variation in the canopy shadow fraction that is independent of changes in LAI or fAPAR. Therefore, results of this work provide a refined basis for understanding how remote sensing can be used to monitor and model the phenology of LAI, fAPAR, APAR, and gross primary productivity in temperate deciduous forests.

54 ENVIRONMENTAL SCIENCES↗

Data-driven prediction of geometry- and toolpath sequence-dependent intra-layer process conditions variations in laser powder bed fusion

Geometrical features and toolpath sequence are two important factors that cause process condition variations, such as variations in the meltpool temperature or meltpool size, that might lead to undesired material properties in the laser powder bed fusion (LPBF) process. Due to the high dynamics and complex physics of the LPBF process, it is difficult to predict variations in process conditions with simulations alone. Advances in measurement technology and computational technologies open up new possibilities for smart manufacturing. In this paper, a data-driven method to predict intra-layer variations in the processing conditions that source from the toolpath sequence and part geometry is presented. The approach is demonstrated using two-color on-axis pyrometer measurements. Three demonstration cases are presented in which it is demonstrated (1) how the trained predictive model can be used as a filter to ease the interpretation of process variations and discover patterns related to toolpath and part geometry, and (2) how to generate predictions that can be used for feedforward control, i.e., for adjusting laser power or scanning speed along the toolpath using a meltpool temperature prediction model generated based on on-axis measurements. Results show that the developed prediction model is able to meaningfully predict process variations resulted from toolpath sequence and geometry. Predictions are aligned with the results from the related work of others and for the case of 180° laser path turnarounds in our high-speed X-ray imaging experiments. In conclusion, the potential issues related to the current maturity status of the process and measuring equipment that could in practice affect the performance of the proposed solutions are also discussed.

42 ENGINEERING↗

A species’ response to spatial climatic variation does not predict its response to climate change

The dominant paradigm for assessing ecological responses to climate change assumes that future states of individuals and populations can be predicted by current, species-wide performance variation across spatial climatic gradients. However, if the fates of ecological systems are better predicted by past responses to in situ climatic variation through time, this current analytical paradigm may be severely misleading. Empirically testing whether spatial or temporal climate responses better predict how species respond to climate change has been elusive, largely due to restrictive data requirements. Here, we leverage a newly collected network of ponderosa pine tree-ring time series to test whether statistically inferred responses to spatial versus temporal climatic variation better predict how trees have responded to recent climate change. When compared to observed tree growth responses to climate change since 1980, predictions derived from spatial climatic variation were wrong in both magnitude and direction. This was not the case for predictions derived from climatic variation through time, which were able to replicate observed responses well. Future climate scenarios through the end of the 21st century exacerbated these disparities. These results suggest that the currently dominant paradigm of forecasting the ecological impacts of climate change based on spatial climatic variation may be severely misleading over decadal to centennial timescales.

54 ENVIRONMENTAL SCIENCES↗

Major impacts of widespread structural variation on sorghum

Genetic diversity is critical to crop breeding and improvement, and dissection of the genomic variation underlying agronomic traits can both assist breeding and give insight into basic biological mechanisms. Although recent genome analyses in plants reveal many structural variants (SVs), most current studies of crop genetic variation are dominated by single-nucleotide polymorphisms (SNPs). The extent of the impact of SVs on global trait variation, as well as their utility in genome-wide selection, is not yet understood. In this study, we built an SV data set based on whole-genome resequencing of diverse sorghum lines (n = 363), validated the correlation of photoperiod sensitivity and variety type, and identified SV hotspots underlying the divergent evolution of cellulosic and sweet sorghum. In addition, we showed the complementary contribution of SVs for heritability of traits related to sorghum adaptation. Importantly, inclusion of SV polymorphisms in association studies revealed genotype–phenotype associations not observed with SNPs alone. Three-way genome-wide association studies (GWAS) based on whole-genome SNP, SV, and integrated SNP + SV data sets showed substantial associations between SVs and sorghum traits. The addition of SVs to GWAS substantially increased heritability estimates for some traits, indicating their important contribution to functional allelic variation at the genome level. Our discovery of the widespread impacts of SVs on heritable gene expression variation could render a plausible mechanism for their disproportionate impact on phenotypic variation. This study expands our knowledge of SVs and emphasizes the extensive impacts of SVs on sorghum.

59 BASIC BIOLOGICAL SCIENCES↗

Sensitivity of modelled passive margin stratigraphy to variations in sea level, sediment supply and subsidence

Abstract We produced a 10 Myr synthetic stratigraphic section using a forward stratigraphic model that generates marine deltaic stratigraphy over geological timescales. We recursively fit the model using a Bayesian inversion algorithm to test: (1) if it could be accurately reconstructed; (2) if the parameters used to create it could be recovered; and (3) the sensitivity of the model output to given model parameters and the attendant physical processes. The original synthetic stratigraphic section was produced with cyclical sea‐level variations of 40 and 30 m with 2.4 and 10 Myr periods respectively. Sediment was also supplied cyclically, in 2.4 and 10 Myr cycles with amplitudes of 30 and 80 tons/100 kyr, respectively, varying from a mean of 232 tons/100 kyr. Parameter values were sampled to fit the model using a Markov chain Monte Carlo algorithm, resulting in a ±5 m (1σ) variation between the experimental output and the original. Sea level varied by ±7 m (1σ) within the posterior distribution of parameters. As a result, both the 10 Myr and 2.4 Myr sea‐level cycles could be extracted from the original output. The variation in sediment supply was approximately ±38 tons/100 kyr (1σ) and, as a result, only the larger long‐term supply variations could be accurately recovered in refitting the model. The variation in thermal, flexural and total subsidence across those parameter sets is less than ±10 m (1σ). The original section experienced 150 m of total subsidence at the depocentre. Our results demonstrate the distinct and interpretable imprint of sea level and subsidence on continental margin stratigraphy can be quantified. Moreover, we conclude that sea‐level change produces a defined effect on the geometries of stratigraphic architecture, and that techniques applied for the purpose of delineating sea‐level variation from continental margin strata have a well‐founded conceptual basis.

Schmelz, William J.↗

Contrasting patterns of sequence variation in steelhead populations reflect distinct evolutionary processes

Abstract Multiple evolutionary processes influence genome‐wide allele frequencies and quantifying effects of genetic drift, and multiple forms of selection remain challenging in natural populations. Here, we investigate variation at major effect loci in contrast to patterns of neutral drift across a wide collection of steelhead ( Oncorhynchus mykiss ) populations that have declined in abundance due to anthropogenic impacts. Whole‐genome resequencing of 74 populations of steelhead revealed genome‐wide patterns (~8 million SNPs) consistent with expected neutral population structure. However, allelic variation at major effect loci associated with adult migration timing (chromosome 28: GREB1L / ROCK1 ) and age at maturity (chromosome 25: SIX6 ) reflected how selection has acted on phenotypic variation in contrast with neutral structure. Variation at major effect loci was influenced by evolutionary processes with differing signals between the strongly divergent Coastal and Inland lineages, while allele frequencies within and among populations within the Inland lineage have been driven by local natural selection as well as recent anthropogenic influences. Recent anthropogenic effects appeared to have influenced the frequency of major effect alleles including artificial selection for specific traits in hatchery stocks with subsequent gene flow into natural populations. Selection from environmental factors at various scales has also likely influenced variation for major effect alleles. These results reveal evolutionary mechanisms that influence allele frequencies at major effect loci that are critical for conservation of phenotypic traits and life history variation of this protected species.

Willis, Stuart↗

Engineering quantitative stomatal trait variation and local adaptation potential by cis‐regulatory editing

Summary Cis‐regulatory element editing can generate quantitative trait variation that mitigates extreme phenotypes and harmful pleiotropy associated with coding sequence mutations. Here, we applied a multiplexed CRISPR/Cas9 approach, informed by bioinformatic datasets, to generate genotypic variation in the promoter ofOsSTOMAGEN, a positive regulator of rice stomatal density. Engineered genotypic variation corresponded to broad and continuous variation in stomatal density, ranging from 70% to 120% of wild‐type stomatal density. This panel of stomatal variants was leveraged in physiological assays to establish discrete relationships between stomatal morphological variation and stomatal conductance, carbon assimilation and intrinsic water use efficiency in steady‐state and fluctuating light conditions. Additionally, promoter alleles were subjected to vegetative drought regimes to assay the effects of the edited alleles on developmental response to drought. Notably, the capacity for drought‐responsive stomatal density reprogramming instomagenand two cis‐regulatory edited alleles was reduced. Collectively our data demonstrate that cis‐regulatory element editing can generate near‐isogenic trait variation that can be leveraged for establishing relationships between anatomy and physiology, providing a basis for optimizing traits across diverse environments.

Biotechnology & Applied Microbiology↗

An Experimental Comparison of Cyclic Variations in Diesel–Natural Gas and POMDME–Natural Gas Dual Fuel Combustion

Abstract Cyclic variations in internal combustion engines are caused by various factors, including combustion mixture stratification, in-cylinder flows, local fluctuations in air-fuel ratio, etc. Cyclic variations have a profound impact on engine performance and emissions. In this study, cyclic variations in dual fuel combustion are analyzed, comparing diesel–natural gas (NG) and polyoxymethylene dimethyl ether (POMDME)-NG dual fuel combustion. Cyclic variability was initially quantified using the coefficient of variation of gross indicated mean effective pressure (IMEPg) computed from experimental cylinder pressure data. The cases analyzed in this study had a coefficient of variation (COV) of IMEPg greater than or around 5%, which was the lower limit of onset of instability for this engine. Experiments were performed at two fixed start of injection (SOI) of high-cetane fuel: 310 CAD and 350 CAD. For all experiments, a constant load of 5 bar IMEPg was maintained, and the intake boost pressure and rail pressure were fixed at 1.5 bar and 500 bar, respectively. For each case, 1000 cycles of cylinder pressure data were recorded, filtered, and processed using an in-house heat release analysis code for each cycle. A comparison between individual cycles and the “ensemble averaged cycle” was made for both diesel–NG and POMDME–NG combustion. For the early SOI of 310 CAD, the peak cylinder pressure fluctuations of individual cycle were found to be ± 15 bar for both fuel combinations, compared to the ensemble averaged cycle, and < 1/10th of the cycles had an IMEPg lower than 0.05 bar of the ensemble averaged cycle. However, the peak pressure fluctuations were found to be lower for POMDME–NG (±3 bar) than diesel–NG dual fuel combustion at 350 CAD SOI, indicating lower cyclic variations. The higher reactivity of POMDME helped reduce fluctuations in combustion phasing at the retarded SOI. The presence of cycles of deterioration and cycles of recovery were also observed with diesel–NG combustion for 310 CAD SOI, and the scatter in the IMEPg return map was similar for both fuel combinations. The IMEPg return map for POMDME–NG combustion was less scattered at the 350 CAD SOI.

Engineering↗

Uncertainties Induced by Processing Parameter Variation in Selective Laser Melting of Ti6Al4V Revealed by In-Situ X-ray Imaging

Selective laser melting (SLM) additive manufacturing (AM) exhibits uncertainties, where variations in build quality are present despite utilizing the same optimized processing parameters. In this work, we identify the sources of uncertainty in SLM process by in-situ characterization of SLM dynamics induced by small variations in processing parameters. We show that variations in the laser beam size, laser power, laser scan speed, and powder layer thickness result in significant variations in the depression zone, melt pool, and spatter behavior. On average, a small deviation of only ~5% from the optimized/reference laser processing parameter resulted in a ~10% or greater change in the depression zone and melt pool geometries. For spatter dynamics, small variation (10 μm, 11%) of the laser beam size could lead to over 40% change in the overall volume of the spatter generated. The responses of the SLM dynamics to small variations of processing parameters revealed in this work are useful for understanding the process uncertainties in the SLM process.

36 MATERIALS SCIENCE↗

A study of the variations in the thermosphere related to solar activity.

Densities derived from the drag of five artificial satellites and covering the time interval from 1958 to 1971 have been used to study the variations in the thermosphere arising from changes in solar activity. Exospheric temperatures derived from the densities by means of atmospheric models have been related to the 10.7-cm solar flux, in which the active-area component had been separated from the slow-varying clear-disk component by means of a relation between the flux and sunspot areas. When the day-to-day (27-day) variation is suppressed, the remaining slow temperature variation appears to be linearly related to the clear-disk flux component. If we also relate linearly the day-to-day temperature variations with the day-to-day variations of the flux, we find that the regression coefficient changes from 1 K at sunspot maximum to 2 K at low solar activity; a nearly constant coefficient is obtained by relating the temperature variations to the ratio of the active-area component to the clear-disk component of the flux.

Jacchia, L. G.↗

The correlation of VLF propagation variations with atmospheric planetary-scale waves

Variations in the received daytime phase of long distance, cesium-controlled, VLF transmission were compared to the height variations of the 10-mb isobaric surface during the first three months of 1965 and 1969. The VLF phase values are also compared to height variations of constant electron densities in the E-region and to variations of f-min which have been shown to be well correlated with planetary-scale variations in the stratosphere by Deland and Cavalieri (1973). The VLF phase variations show good correlation with these previous ionospheric measurements and with the 10-mb surfaces. The planetary scale waves in the stratosphere are shown to be travelling on the average eastward in 1965 and westward in 1969. These correlations are interpreted as due to the propagation of travelling planetary scale waves with westward tilted wave fronts. Upward energy transport due to the vertical structure of those waves is also discussed. These correlations provide further evidence for the coupling between the lower ionosphere at about 70 km altitude (the daytime VLF reflection height and the stratosphere, and they demonstrate the importance of planetary wave phenomena to VLF propagation.

Cavalieri, D. J.↗

Turbine vane coolant flow variations and calculated effects on metal temperatures

Seventy-two air-cooled turbine vanes were tested to determine coolant flow variations among the vanes. Calculations were made to estimate the effect of measured coolant flow variations on local vane metal temperatures. The calculations were based on the following assumed operating conditions: turbine inlet temperature, 1700 K (2600 F); turbine inlet pressure, 31 N/sq cm (45 psia); coolant inlet temperature, 811 K (1000 F); and total coolant to gas flow ratio, 0.065. Variations of total coolant flow were not large (about 10 percent from the arithmetic mean) for all 72 vanes, but variations in local coolant flows were large. The local coolant flow variations ranged from 8 to 75 percent, and calculated metal temperature variations ranged from 8 to 60 K (15 to 180 F).

Yeh, F. C.↗

Effects of granular convection in the response of C I 5380 A to solar luminosity variations

The response of the weak solar photospheric neutral carbon line at 5380.3 A to percolations of photospheric granulation and the five-minute solar oscillations is investigated in order to calibrate the sensitivity of the line to temporal variations in solar luminosity. Line strength variations and simultaneous continuum variations in two granular regions were observed at 30 sec intervals using a 25-cm coude spectrograph. The response of line equivalent width to continuum intensity variations is found to be essentially uniform at time scales ranging from 5 min to 2 hr independently of whether the variations arise from granular convection or velocity field oscillations. The extent to which line strength varies with luminosity (sensitivity) is observed to be significantly smaller than estimates based on models of the perturbed Harvard-Smithsonian Reference Atmosphere of Gingerich et al. (1970), and it is proposed that the structure of the solar granulation is a major factor determining local luminosity variations and line sensitivity.

Lindsey, C. A.↗

The influence of solar UV variations on climate

The effects on the terrestrial ozone abundance and temperature (and hence on the earth's climate) of periodic variations in the solar spectrum are investigated. Temporal variations of the solar UV spectrum are modeled in accordance with the measurements of Heath and Thekaekara (1977), and the spectrum at wavelengths greater than 2900 A is altered uniformly by small amounts so that the total luminosity remains constant with time. One-dimensional photochemical-radiative-convective models are used to predict the response of the earth's surface to the solar spectral changes. Results show that the data interpreted by Heath and Thekaekara to indicate that the solar UV flux varies by a factor of 2.5 at 1750 A from solar minimum to solar maximum are inconsistent with the historical records of ozone abundance. It is concluded, however, that if the amplitude of solar UV variations increases with increasing period, and if the spectral characteristics variations are similar to the well established solar UV variations over a solar rotation period, these variations could have a significant impact on the earth's climate and the biosphere.

Borucki, W. J.↗

Jovimagnetic secular variation

Long term variations of a planetary magnetic field are one of the few observables available in the study of planetary interiors and dynamo theory. While variations of the geomagnetic field were accessible to direct measurement for centuries, knowledge of the secular variations of other planetary dynamos is limited. New limits on Jovimagnetic secular variations were found by comparison of a Jovian internal field model obtained from the Voyager 1 magnetic field observations at epoch 1979.2 with the epoch 1974.9 Pioneer 11 O4 model. No significant secular variation of either the magnitude or position of the Jovidipole is found for the years 1974.9 through 1979.2, although a small Earth-like variation cannot be ruled out.

Connerney, J. E. P.↗

Interstellar turbulence, random density variations, and scintillation measurements

The presence of random electron variations suggests that the ionized interstellar medium is turbulent. In the interstellar plasma the presence of power spectra of such variations extending to spatial scales much less than a Coulomb mean free path, Lambda sub c, is required by analyses of measurements of scintillation and angular broadening of pulsar radio signals. The existence of corresponding variations in magnetic field strength could efficiently scatter cosmic rays and thus constrain cosmic-ray propagation. Unfortunately both the origin of the electron density variations and mechanisms by which these variations couple to fluctuations in magnetic field strength are unknown. It is conjectured that the small-scale density variations are generated by the convective distortion of initially large-scale isobaric entropy structures in the turbulent interstellar plasma. An investigation of the spectra of turbulent entropy structures, velocity, and magnetic fields at small spatial scales is made. The modifier small is employed to characterize length scales much less than the dimension, L, containing the bulk of the turbulent energy.

Higdon, J. C.↗

Diurnal variation of outgoing longwave radiation in the tropics

The diurnal harmonic in longwave emission in the tropical belt (30 deg N-30 deg S) is estimated from nine years of NOAA polar-orbiting satellite data. The results are compared successfully with Nimbus-7 ERB scanner data and with GOES-West geosynchronous satellite data. An interesting and consistent diurnal variation in longwave emission is found over the regions of intense oceanic convection, such as the ITCZ and SPCZ regions, with a peak-to-peak variation of 6-8 W/sq m and a maximum in the morning (0600-1200 LST). Histogram analysis indicates that this variation is associated with a diurnal variation in convective cloud (about 400 mb). Over regions of very intense convection, a diurnal variation of very high clouds (above 100 mb), which is out of phase with the variations at lower levels in the atmosphere, reduces the magnitude of the diurnal harmonic in longwave emission. It is interesting that histograms based on data averaged over 8-km and 250-km boxes give the same qualitative information about cloud and emission variability.

Hartmann, D. L.↗

Sub-second variations of high energy ( 300 keV) hard X-ray emission from solar flares

Subsecond variations of hard X-ray emission from solar flares were first observed with a balloon-borne detector. With the launch of the Solar Maximum Mission (SMM), it is now well known that subsecond variations of hard X-ray emission occur quite frequently. Such rapid variations give constraints on the modeling of electron energization. Such rapid variations reported until now, however, were observed at relatively low energies. Fast mode data obtained by the Hard X-ray Burst Spectrometer (HXRBS) has time resolution of approximately 1 ms but has no energy resolution. Therefore, rapid fluctuations observed in the fast-mode HXRBS data are dominated by the low energy hard X-rays. It is of interest to know whether rapid fluctuations are observed in high-energy X-rays. The highest energy band at which subsecond variations were observed is 223 to 1057 keV. Subsecond variations observed with HXRBS at energies greater than 300 keV are reported, and the implications discussed.

Bai, Taeil↗