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At least 109 records · Page 6

Microorganisms, Organic Carbon, and Their Relationship with Oxidant Activity in Hyper-Arid Mars-Like Soils: Implications for Soil Habitability

Soil samples from the hyper-arid region in the Atacama 23 Desert in Southern Peru (La Joya Desert) were analyzed for total and labile organic carbon (TOC & LOC), phospholipid fatty acids analysis (PLFA), quantitative real time polymerase chain reaction (qRT-PCR), 4',6- diamidino-2-phenylindole (DAPI)-fluorescent microscopy, culturable microorganisms, and oxidant activity, in order to understand the relationship between the presence of organic matter and microorganisms in these types of soils. TOC content levels were similar to the labile pool of carbon suggesting the absence of recalcitrant carbon in these soils. The range of LOC was from 2 to 60 micro-g/g of soil. PLFA analysis indicated a maximum of 2.3 x 10(exp 5) cell equivalents/g. Culturing of soil extracts yielded 1.1 x 10(exp 2)-3.7 x 10(exp 3) CFU/g. qRT-PCR showed between 1.0 x 10(exp 2) and 8 x 10(exp 3) cells/g; and DAPI fluorescent staining indicated bacteria counts up to 5 x 104 cells/g. Arid and semiarid samples (controls) showed values between 10(exp 7) and 10(exp 11) cells/g with all of the methods used. Importantly, the concentration of microorganisms in hyper-arid soils did not show any correlation with the organic carbon content; however, there was a significant dependence on the oxidant activity present in these soil samples evaluated as the capacity to decompose sodium formate in 10 hours. We suggest that the analysis of oxidant activity could be a useful indicator of the microbial habitability in hyper-arid soils, obviating the need to measure water activity over time. This approach could be useful in astrobiological studies on other worlds.

Mars-like soils↗

Carbon dioxide, water vapor and methane soil efflux (soil respiration) in a Pinus palustris restoration site in Georgetown, SC

This dataset contains processed data from a combination of survey flux chambers and long-term automated flux chambers. Biweekly soil flux measurements were conducted from June 2023 through December 2025 at a longleaf pine restoration site in Georgetown, SC. Processed, QAQC’d data can be found in the file: 1_DATA_ESS_DOE_HR_RS_HB3_QAQC_Survey_Data_20260223.csv. Raw and working data files (.json, & .81x format) from LI-COR equipment are included for reference and can be accessed using SoilFluxPro software. CSV metadata files describe the raw data and modifications made using SoilFluxPro v5 and Matlab R2024b, as well as formatting and units for processed CSVs. Matlab code is included for reading in the processed CSVs. This research was performed as part of the project: “Improving models of stand and watershed carbon and water fluxes with more accurate representations of soil-plant-water dynamics in southern pine ecosystems”, which examines in part the effects hydraulic redistribution on soil efflux of carbon dioxide, water vapor and methane, as well as soil moisture and temperature in a southern pine ecosystem with sandy soils and high water table.

CARBON DIOXIDE FLUX↗

Post-fire soil respiration in late growing season (2023 and 2024), Kougarok Fire Complex, Seward Peninsula, Alaska

Field soil respiration data collected in 2023 and 2024 from burned and unburned tussock tundra sites in the Kougarok Fire Complex, near Nome, on the Seward Peninsula of Alaska. Specifically, we measured soil properties and late-growing season CO2 fluxes in patches of unique plant functional types (forbs, shrubs, and graminoids) across two years in tundra recovering from repeated wildfires over the decade. The goal was to identify the main drivers of soil respiration in Arctic tundra underlain by discontinuous permafrost that is recovering from two recent, repeated wildfires that differed in fire age and number of times burned, thereby resulting in different levels of vegetation and subsurface property changes (i.e., successional trajectories). There are five files in *.csv format with one data file and four data description files including data, dictionary, methods, terminology, and file-level metadata. The Next-Generation Ecosystem Experiments: Arctic (NGEE Arctic), is a research effort to reduce uncertainty in Earth System Models by developing a predictive understanding of carbon-rich Arctic ecosystems and feedbacks to climate. NGEE Arctic was supported by the Department of Energy's Office of Biological and Environmental Research. The NGEE Arctic Phase 3 project had two field research sites: 1) located within the Arctic polygonal tundra coastal region on the Barrow Environmental Observatory (BEO) and the North Slope near Utqiagvik (Barrow), Alaska and 2) multiple areas on the discontinuous permafrost region of the Seward Peninsula north of Nome, Alaska. Through observations, experiments, and synthesis with existing datasets, NGEE Arctic provided an enhanced knowledge base for multi-scale modeling and contributed to improved process representation at global pan-Arctic scales within the Department of Energy's Earth system Model (the Energy Exascale Earth System Model, or E3SM), and specifically within the E3SM Land Model component (ELM).

Santos, Fernanda [ORNL] (ORCID:0000000191555623)↗

Toward a standardization of cryostructure and cryogenic soil structure terminology for the field description of permafrost‐affected soils

This paper establishes standardized terminology and field documentation protocols for cryostructures and cryogenic soil structures in permafrost‐affected soils and provides brief guidance on descriptions of ground ice morphology and ice volume estimates. We consolidate permafrost terminology from Russian and North American literature, clarify long‐standing ambiguities, and provide explicit guidelines that align with US Department of Agriculture‐Natural Resources Conservation Service soil description standards. Our scheme makes critical distinctions between cryostructure, the distribution of ice within soil, and cryogenic soil structure, the morphological structure of soil resulting from ice formation. The scheme organizes cryostructures into three main categories: non‐segregated ice, visible segregated ice, and ice matrices. We introduce standardized codes and parameters for field descriptions of ice and soil that enable machine‐readable data collection compatible with existing soil information systems. This standardization will significantly enhance the integration of field observations into landscape‐scale assessments of permafrost stability, infrastructure vulnerability, and ecosystem response to permafrost thaw, addressing an urgent need for quantitative data to inform modeling and decision‐making in rapidly changing Arctic and subarctic environments.

Andersen, Megan L. [University of Minnesota, Saint↗

On the uncertainty of estimating photovoltaic soiling using nearby soiling data

The accumulation of dust on the surface of photovoltaic modules can reduce their performance and affect the cost competitiveness of this technology. This phenomenon is known as soiling and can be mitigated through appropriate corrective and/or preventive actions. In order to maximize its effectiveness, it is important to plan the soiling mitigation strategy even before the PV system is operational. This is typically done through a nearest neighbor approach, by estimating soiling using data from the nearest operational photovoltaic system. This work focuses on understanding the uncertainty related to this practice. For this purpose, the semi-variance function is used to study the dissimilarity between the soiling losses of two locations in California depending on their distance. The results show that, when the soiling loss at a nearby system is used to estimate soiling of a site, the uncertainty can be approximated to increase linearly at a rate of 0.08-0.10%/km up to 60 or 80 km. After this distance, the use of a nearest neighbor approach is no longer justified, as it produces an uncertainty as big as the average soiling loss of the sites in the dataset used in this study. In some conditions, uncertainties > 0% are found also for sites located within 25 km, meaning that even close-by systems might soil differently.

14 SOLAR ENERGY↗

Panama Rainforest Changes with Experimental Drought (PaRChED): Initial Effects of Partial Throughfall Exclusion on Soil Dynamics in Lowland Forests Across Variation in Rainfall and Soil Fertility

Changes in rainfall are predicted across tropical regions, with effects on nutrient, water, and carbon cycling. This chapter summarizes results from the first two years of a throughfall exclusion experiment in four lowland Panamanian forests that span a 1,000-mm change in rainfall and variation in soil fertility. Soil respiration (i.e., soil carbon dioxide [CO2 ] flux) declined with throughfall exclusion, with a site*season interaction, and the radiocarbon age of respired carbon was older in exclusion versus control plots. The decline in soil CO2 flux could be related to reduced fine root production and soil microbial biomass. Microbial community composition also changed with throughfall exclusion in infertile soils, and soil nutrients accumulated more in exclusion versus control plots during the dry season. The net effects on soil carbon storage will depend on the relative strengths of these effects over time. Continued research could improve predictions of tropical forest-climate feedbacks with changes in precipitation.

54 ENVIRONMENTAL SCIENCES↗

Characterizing suburban soil and microbial properties along a soil age chronosequence

Abstract Rapid urbanization is drastically altering ecosystem processes in landscapes around the world. In particular, suburban residential neighborhoods comprise novel ecosystems with water and nutrient inputs that differ greatly from the surrounding land area. These impacts generate concern over the sustainability of urban ecosystems, especially whether they will be characterized by net carbon gain or loss over time. To address this knowledge gap, we established a chronosequence of residential yards in Southern California to test how urban soils change after development. We predicted that urbanized soils would experience shifts in physical characteristics and microbial function over time consistent with ecological succession theory, but residential soils would maintain novel moisture and nutrient regimes compared to undeveloped soils, never “recovering” to a pre‐developed state. We compared different vegetation types to quantify impacts of homeowner landscaping choices and characterized yard soils and their microbial communities. We found that yard soils were nutrient‐ and moisture‐enriched compared to an adjacent undeveloped ecosystem, and turfgrass was associated with higher levels of water and nitrogen. Despite high respiration rates, yard soils accumulated carbon and nitrogen over time. We conclude that suburban residential soils comprise dynamic and heterogeneous ecosystems that are highly influenced by landscaping choices and management practices, and warrant closer study at small management‐relevant scales.

Suratt, Andie [Department of Ecology and Evolution↗

Alternating salt and freshwater floods of coastal soils impact soil structure, hydraulic properties, and oxygen dynamics

Coastal soils are increasingly impacted by hydrologic intensification in the form of rising sea level and flooding from storm surges and precipitation. Alternating saltwater (SW) and freshwater (FW) exposure has the potential to disperse colloids, which can lead to disintegration of soil structure, clogging of pore spaces, and reduction in ecologically and biogeochemically important functions like infiltration or gas exchange. To investigate how hydrologic intensification affects soil structure and oxygen dynamics, we conducted a series of laboratory-based flood simulations. Intact soil cores of the A and B horizons (22 total) from the toe slope of an upland coastal forest along the western shore of Chesapeake Bay. We subjected the cores to 24 h of saturation with either FW or alternating brackish SW and FW, with a 24-h draining event in between flood events. Oxygen diffusion into soil during draining was reduced by up to 30% for soils flooded alternating SW-FW compared to soils flooded only with FW. We attributed the reduced oxygen diffusion to clogging of smaller pores by colloids, with colloid redistribution observed as an increase in specific surface area down the core profile of up to 59%. After three SW-FW floods (six floods total), there were significant changes in pore size distribution, significant redistribution of colloids, and the A horizon became sodic. We concluded that a small number of SW flooding events can induce a measurable change in soil physical properties that directly impacts the biogeochemical dynamics.

Rod, Kenton A. [Pacific Northwest National Laborat↗

Intensified Soil Moisture Extremes Decrease Soil Organic Carbon Decomposition: A Mechanistic Modeling Analysis

Earth system models have predicted that there will be more frequent and severe precipitation and drought events in terrestrial ecosystems. Microbially mediated decomposition of soil organic carbon (SOC) tends to increase as soils wet and decrease as soils dry. However, the long-term SOC change under intensified moisture extremes remains poorly known as it depends on the frequency and intensity of soil drying and wetting. In this study, we explored long-term SOC dynamics under scenarios of alternating drying-wetting cycles using the Microbial-ENzyme Decomposition model, a mechanistic microbial model. The model was parameterized with 11 years of observations from a temperate deciduous broadleaf forest site, showing satisfactory model performance in both model calibration (R 2 = 0.67) and validation (R 2 = 0.69) against heterotrophic respiration. We then used the model to simulate the long-term SOC dynamics under five scenarios of alternating drying-wetting cycles with different frequencies and severities over a period of 100 years. Results showed that the changes in active microbial biomass C and the corresponding turnover rates of SOC pools were more sensitive to soil drying than soil wetting. As a result, the cumulative soil carbon emission from microbial respiration decreased by 433.7 g C m -2 after the 100-year simulation in the highest frequency and intensity moisture scenario, but was not significantly affected by the lowest frequency and intensity scenario. This study emphasizes the nonlinear response of SOC decomposition to soil moisture changes, which causes decreased decomposition by microbes under drying that is, not compensated by increased decomposition under wetting conditions.

58 GEOSCIENCES↗

Tendencies of Soil Microbial NO Emissions During HI‐SCALE as Predicted by a Nitrification/Denitrification Scheme

Many atmospheric chemical processes, including the formation of secondary organic aerosol (SOA), are strongly modulated by the reactions of NO and NO 2 (NO x ). Though NO x is controlled by anthropogenic emissions near urban areas, in rural areas soil microbes can be a significant contribution to NO emission globally. The relative rates of emissions of different nitrogen-containing species (e.g., NO, N 2 O, HONO, and N 2 ) are strong functions of soil properties such as temperature, moisture content, pH, and soil carbon and nitrogen pools. However, typical large-scale biogeochemical models either express these emissions simplistically, or not at all. Here we investigate the potential impact of soil NO emissions on atmospheric chemistry and SOA formation over regional and monthly time scales, specifically the 2016 spring and summer Intensive Observational Periods (IOPs) of the Holistic Interactions of Shallow Clouds, Aerosols and Land Ecosystems (HI-SCALE) field campaign. We implement the soil NO nitrification/denitrification parameterization of Rasool et al. (2019) into the Weather Research and Forecasting model coupled with Chemistry (WRF-Chem), supplemented by a 1-km soil moisture analysis. We then simulate both IOPs over the U.S. Great Plains, evaluating against ground stations and flight data. We show that soil NO emissions can account for a large fraction of total NO x and locally increase O 3 concentrations by up to 25%, while alleviating negative biases of gases and aerosols toward observations. Soil moisture and temperature changes between IOP1 and IOP2 lead to overall differences in emissions, but with large regional variability due to heterogeneous surface characteristics.

Atmoshpheric Chemistry↗

Soil viruses: Understudied agents of soil ecology

Over the past couple decades there has been intense exploration of the soil microbiome using modern sequencing and bioinformatics approaches. These studies have revealed a vast diversity of microorganisms across a variety of habitats (Thompson et al., 2017) and how differences in the environment, for example with climate change, influence the soil microbial community composition (Jansson and Hofmockel, 2020). The focus of these studies has primarily been on microbial cellular organisms, including bacteria, archaea and fungi. By contrast, in depth study of soil viruses has been largely neglected, until recently. There are several reasons that soil viruses are less studied, including difficulties with extraction of viruses from soil particles, difficulties with classification of soil viruses based on microscopic imaging, and reliance on the small proportion of microbial hosts that can be cultivated. Now that comprehensive soil metagenomes are available the current trend has been to survey the metagenomes for viral sequences. This approach has revealed that soil viruses are incredibly abundant, highly diverse, and largely uncharacterized (Paez-Espino et al., 2016). This so-called ‘viral dark matter’ presents an incredible research opportunity for the future. Here I will highlight some of these potential research avenues based on what is known to date.

54 ENVIRONMENTAL SCIENCES↗

Relation between L-band soil emittance and soil water content

An experimental relation between soil emittance (E) at L-band and soil surface moisture content (M) is compared with a theoretical one. The latter depends on the soil dielectric constant, which is a function of both soil moisture content and of soil texture. It appears that a difference of 10 percent in the surface clay content causes a change in the estimate of M on the order of 0.02 cu m/cu m. This is based on calculations with a model that simulates the flow of water and energy, in combination with a radiative transfer model. It is concluded that an experimental determination of the E-M relation for each soil type is not required, and that a rough estimate of the soil texture will lead to a sufficiently accurate estimate of soil moisture from a general, theoretical relationship obtained by numerical simulation.

Stroosnijder, L.↗

Spatial and temporal variability of soil temperature, moisture and surface soil properties

The overall objectives of this research were to: (l) Relate in-situ measured soil-water content and temperature profiles to remotely sensed surface soil-water and temperature conditions; to model simultaneous heat and water movement for spatially and temporally changing soil conditions; (2) Determine the spatial and temporal variability of surface soil properties affecting emissivity, reflectance, and material and energy flux across the soil surface. This will include physical, chemical, and mineralogical characteristics of primary soil components and aggregate systems; and (3) Develop surface soil classes of naturally occurring and distributed soil property assemblages and group classes to be tested with respect to water content, emissivity and reflectivity. This document is a report of studies conducted during the period funded by NASA grants. The project was designed to be conducted over a five year period. Since funding was discontinued after three years, some of the research started was not completed. Additional publications are planned whenever funding can be obtained to finalize data analysis for both the arid and humid locations.

Hajek, B. F.↗

Application of Multitemporal Remotely Sensed Soil Moisture for the Estimation of Soil Physical Properties

This paper demonstrates the use of multitemporal soil moisture derived from microwave remote sensing to estimate soil physical properties. The passive microwave ESTAR instrument was employed during June 10-18, 1992, to obtain brightness temperature (TB) and surface soil moisture data in the Little Washita watershed, Oklahoma. Analyses of spatial and temporal variations of TB and soil moisture during the dry-down period revealed a direct relationship between changes in T and soil moisture and soil physical (viz. texture) and hydraulic (viz. saturated hydraulic conductivity, K(sat)) properties. Statistically significant regression relationships were developed for the ratio of percent sand to percent clay (RSC) and K(sat), in terms of change components of TB and surface soil moisture. Validation of results using field measured values and soil texture map indicated that both RSC and K(sat) can be estimated with reasonable accuracy. These findings have potential applications of microwave remote sensing to obtain quick estimates of the spatial distributions of K(sat), over large areas for input parameterization of hydrologic models.

Mattikalli, N. M.↗

Space Weathering of Intermediate-Size Soil Grains in Immature Apollo 17 Soil 71061

Understanding space weathering, which is caused by micrometeorite impacts, implantation of solar wind gases, radiation damage, chemical effects from solar particles and cosmic rays, interactions with the lunar atmosphere, and sputter erosion and deposition, continues to be a primary objective of lunar sample research. Electron beam studies of space weathering have focused on space weathering effects on individual glasses and minerals from the finest size fractions of lunar soils [1] and patinas on lunar rocks [2]. We are beginning a new study of space weathering of intermediate-size individual mineral grains from lunar soils. For this initial work, we chose an immature soil (see below) in order to maximize the probability that some individual grains are relatively unweathered. The likelihood of identifying a range of relatively unweathered grains in a mature soil is low, and we plan to study grains ranging from pristine to highly weathered in order to determine the progression of space weathering. Future studies will include grains from mature soils. We are currently in the process of documenting splash glass, glass pancakes, craters, and accretionary particles (glass and mineral grains) on plagioclase from our chosen soil using high-resolution field emission scanning electron microscopy (FESEM). These studies are being done concurrently with our studies of patinas on larger lunar rocks [e.g., 3]. One of our major goals is to correlate the evidence for space weathering observed in studies of the surfaces of samples with the evidence demonstrated at higher resolution (TEM) using cross-sections of samples. For example, TEM studies verified the existence of vapor deposits on soil grains [1]; we do not yet know if they can be readily distinguished by surfaces studies of samples. A wide range of textures of rims on soil grains is also clear in TEM [1]; might it be possible to correlate them with specific characteristics of weathering features seen in SEM?

Wentworth, S. J.↗

Improving Simulated Soil Moisture Fields Through Assimilation of AMSR-E Soil Moisture Retrievals with an Ensemble Kalman Filter and a Mass Conservation Constraint

Model simulated soil moisture fields are often biased due to errors in input parameters and deficiencies in model physics. Satellite derived soil moisture estimates, if retrieved appropriately, represent the spatial mean of soil moisture in a footprint area, and can be used to reduce model bias (at locations near the surface) through data assimilation techniques. While assimilating the retrievals can reduce model bias, it can also destroy the mass balance enforced by the model governing equation because water is removed from or added to the soil by the assimilation algorithm. In addition, studies have shown that assimilation of surface observations can adversely impact soil moisture estimates in the lower soil layers due to imperfect model physics, even though the bias near the surface is decreased. In this study, an ensemble Kalman filter (EnKF) with a mass conservation updating scheme was developed to assimilate the actual value of Advanced Microwave Scanning Radiometer (AMSR-E) soil moisture retrievals to improve the mean of simulated soil moisture fields by the Noah land surface model. Assimilation results using the conventional and the mass conservation updating scheme in the Little Washita watershed of Oklahoma showed that, while both updating schemes reduced the bias in the shallow root zone, the mass conservation scheme provided better estimates in the deeper profile. The mass conservation scheme also yielded physically consistent estimates of fluxes and maintained the water budget. Impacts of model physics on the assimilation results are discussed.

Li, Bailing↗

Soil Temperature Sensor Data, 2025, Five sites in Knoxville, Tennessee

This dataset contains surface soil temperature measurements from five urban parks in Knoxville, Tennessee: Cumberland Estates Park (CE), Socially Equal Energy Efficient Development (SD), West View Park (WV), Victor Ashe Park (VA), and West Hills Park (WH). The dataset includes 16 CSV files documenting soil temperature measurements recorded by HOBO Pendant MX Water Temperature Data Loggers. Data collection for all sites began on January 1, 2025. The end time for each sensor is provided in the End Time_2025.csv file. Each logger was installed at a depth of 10 inches and positioned approximately 3 to 6 feet from the weather station at each site. This dataset is part of a broader study examining the effects of soil moisture and plant evapotranspiration on ambient temperature and relative humidity across multiple urban parks in Knoxville.

Salvador, Christian [ORNL] (ORCID:0000000283287777↗

Earthworms significantly enhance the temperature sensitivity of soil organic matter decomposition: Insights into future soil carbon budgeting

How and what soil fauna influence the soil organic matter (SOM) decomposition rate (Rs) and its temperature sensitivity (Q 10 ) have been largely ignored, although this is a crucial matter, especially under the scenario of global change. Further, in this study, a novel approach was adopted with a continuous changing-temperature incubation (daytime, from 7 °C to 22 °C; nighttime, from 22 °C to 7 °C) with rapid and continuous measurement, to examine the effect of soil macrofauna (specifically, earthworms) on Rs and Q 10 with three densities (no addition, low density, and high density). According to the results, the earthworms accelerated Rs. Furthermore, Rs with earthworm addition had a symmetrical pattern during daytime and nighttime cycles, which is contrary to traditional soil incubation, with only soil microbe as asymmetrical. More importantly, earthworm addition increased Q 10 markedly, ranging from 48% to 67%. Overall, the findings highlight the pivotal role of earthworms as soil macrofauna that regulating soil carbon release, and their effects should be integrated into process-based ecological models in future.

60 APPLIED LIFE SCIENCES↗