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Results for “Sensitivity and Specificity”

Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 109 records · Page 6
All sourcesNASA NTRSDOE OSTI
DOE OSTI2024

Surface-Enhanced Raman Spectroscopy Combined with Multivariate Analysis for Fingerprinting Clinically Similar Fibromyalgia and Long COVID Syndromes

Fibromyalgia (FM) is a chronic central sensitivity syndrome characterized by augmented pain processing at diffuse body sites and presents as a multimorbid clinical condition. Long COVID (LC) is a heterogenous clinical syndrome that affects 10–20% of individuals following COVID-19 infection. FM and LC share similarities with regard to the pain and other clinical symptoms experienced, thereby posing a challenge for accurate diagnosis. This research explores the feasibility of using surface-enhanced Raman spectroscopy (SERS) combined with soft independent modelling of class analogies (SIMCAs) to develop classification models differentiating LC and FM. Venous blood samples were collected using two supports, dried bloodspot cards (DBS, n = 48 FM and n = 46 LC) and volumetric absorptive micro-sampling tips (VAMS, n = 39 FM and n = 39 LC). A semi-permeable membrane (10 kDa) was used to extract low molecular fraction (LMF) from the blood samples, and Raman spectra were acquired using SERS with gold nanoparticles (AuNPs). Soft independent modelling of class analogy (SIMCA) models developed with spectral data of blood samples collected in VAMS tips showed superior performance with a validation performance of 100% accuracy, sensitivity, and specificity, achieving an excellent classification accuracy of 0.86 area under the curve (AUC). Amide groups, aromatic and acidic amino acids were responsible for the discrimination patterns among FM and LC syndromes, emphasizing the findings from our previous studies. Overall, our results demonstrate the ability of AuNP SERS to identify unique metabolites that can be potentially used as spectral biomarkers to differentiate FM and LC.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2024

Exposed Phosphatidylserine as a Biomarker for Clear Identification of Breast Cancer Brain Metastases in Mouse Models

Brain metastasis is the most common intracranial malignancy in adults. The prognosis is extremely poor, partly because most patients have more than one brain lesion, and the currently available therapies are nonspecific or inaccessible to those occult metastases due to an impermeable blood–tumor barrier (BTB). Phosphatidylserine (PS) is externalized on the surface of viable endothelial cells (ECs) in tumor blood vessels. In this study, we have applied a PS-targeting antibody to assess brain metastases in mouse models. Fluorescence microscopic imaging revealed that extensive PS exposure was found exclusively on vascular ECs of brain metastases. The highly sensitive and specific binding of the PS antibody enables individual metastases, even micrometastases containing an intact BTB, to be clearly delineated. Furthermore, the conjugation of the PS antibody with a fluorescence dye, IRDye 800CW, or a radioisotope, 125I, allowed the clear visualization of individual brain metastases by optical imaging and autoradiography, respectively. In conclusion, we demonstrated a novel strategy for targeting brain metastases based on our finding that abundant PS exposure occurs on blood vessels of brain metastases but not on normal brain, which may be useful for the development of imaging and targeted therapeutics for brain metastases.

Oncology↗
NASA NTRS1965

Dynamic buckling estimates.

Dynamic buckling of imperfection sensitive models, specifically cylindrical shells under axial compression

AXIAL COMPRESSION↗
NASA NTRS1966

Dynamic buckling estimates.

Dynamic buckling of imperfection sensitive models, specifically cylindrical shells under axial compression

AXIAL COMPRESSION↗
NASA NTRS1965

Speculations on smell.

Experimental research into olfactive code, noting psychological and physiological analysis, suggesting specific chemically sensitive molecules role

ELECTROPHYSIOLOGY↗
NASA NTRS1972

Advanced techniques for determining long term compatibility of materials with propellants

The search for advanced measurement techniques for determining long term compatibility of materials with propellants was conducted in several parts. A comprehensive survey of the existing measurement and testing technology for determining material-propellant interactions was performed. Selections were made from those existing techniques which were determined could meet or be made to meet the requirements. Areas of refinement or changes were recommended for improvement of others. Investigations were also performed to determine the feasibility and advantages of developing and using new techniques to achieve significant improvements over existing ones. The most interesting demonstration was that of the new technique, the volatile metal chelate analysis. Rivaling the neutron activation analysis in terms of sensitivity and specificity, the volatile metal chelate technique was fully demonstrated.

Green, R. L.↗
NASA NTRS1972

Urinary Excretion of Antidiuretic Hormone in Man

It is shown that urinary excretion of ADH can be detected readily and quantitated accurately. The ADH excretion in normal subjects is inhibited following the administration of a water load and stimulated following water deprivation. It appears that measurement of ADH excretion in man provides a means of quantitating alterations in neurohypophyseal ADH secretion. By determining not only the basal excretion of ADH but also the response to such physiological influences as water loading and dehydration, it becomes possible to study the dynamics of ADH release. Thus, the ability to extract ADH efficiently from urine combined with a sensitive and specific technique for determination of ADH concentration allows the exploration of regulatory systems for ADH control in the normal state as well as the etiological role of altered ADH secretion in clinical disorders of water balance.

Miller, M.↗
NASA NTRS1974

The determination of ethanol in blood and urine by mass fragmentography

A mass fragmentographic technique for a rapid, specific and sensitive determination of ethanol in blood and urine is described. A Varian gas chromatograph coupled through an all-glass membrane separator to a Finnigan quadripole mass spectrometer and interfaced to a computer system is used for ethanol determination in blood and urine samples. A procedure for plotting calibration curves for ethanol quantitation is also described. Quantitation is achieved by plotting the peak area ratios of undeuterated-to-deuterated ethanol fragment ions against the amount of ethanol added. Representative results obtained by this technique are included.

Pereira, W. E.↗
NASA NTRS1975

Development of a chemiluminescent and bioluminescent system for the detection of bacteria in wastewater effluent

Automated chemiluminescent and bioluminescent sensors were developed for continuous monitoring of microbial levels in wastewater effluent. Development of the chemiluminescent system included optimization of reagent concentrations as well as two new techniques which will allow for increased sensitivity and specificity. The optimal reagent concentrations are 0.0025 M luminol and 0.0125 M sodium perborate in 0.75N sodium hydroxide before addition of sample. The methods developed to increase specificity include (1) extraction of porphyrins from bacteria collected in a filter using 0.1N NaOH - 50 percent Ethanol, and (2) use of the specific reaction rate characteristics for the different luminol catalysts. Since reaction times are different for each catalyst, the reaction can be made specific for bacteria by measuring only the light emission from the particular reaction time zone specific for bacteria. Developments of the bioluminescent firefly luciferase system were in the area of flow system design.

Thomas, R. R.↗
NASA NTRS1976

Geochemistry of biomolecules

A highly sensitive fluorometric technique is developed for the determination of biological and geo-organic compounds in ancient sediments and extraterrestrial samples. This technique is used to establish chemical evidence for fossil pigments in an extraterrestrial sample. Also developed is a highly sensitive and specific fluorometric method for the determination of total primary amine nitrogen in soil samples.

Bonner, J.↗
NASA NTRS1977

Airborne HCl - CO sensing system

A system for measuring air pollutants in-situ using an aircraft was designed, fabricated, and tested. The system is based upon a technique called Gas Filter Correlation (GFC) which provides for high sensitivity and specificity in the presence of interfering species. This particular system was designed for measuring hydrochloric acid and carbon monoxide gases emitted from rocket exhaust effluents.

Bartle, E. R.↗
NASA NTRS1983

Definition of Forces on Turbomachinery Rotors. Task B Report: Dynamic Analysis of Rotors

The rotordynamic characteristics of turbomachinery are known to depend on the forces developed due to relative motion between the rotor and the housing. For example, the critical speed locations generally depend on the bearing stiffnesses, seal dampling influences rotor stability and bearing reaction amplitudes near critical speeds, etc. A systematic examination of the influence of changes in the forces acting on rotors is studied. More specifically, the sensitivity of the rotordynamic characteristics to changes in rotor forces is analyzed. Rotordynamic characteristics of the HPOTP (High Pressure Oxygen Turbopump) and HPFTP (High Pressure Fuel Turbopump) of the SSME (Space Shuttle Main Engine) are investigated. Because of their markedly different rotordynamic characteristics, these units are considered to be representative of a range of possible future liquid rocket engine turbomachinery.

Childs, D. W.↗
NASA NTRS1982

General contamination criteria for optical surfaces

Physical models are developed for establishing criteria to decide on the acceptable contamination level of optical devices in space-borne conditions. Optical systems can be degraded in terms of decreased throughput, i.e., transmissivity or reflectivity, or increases in the total integrated scatter (TIS). Performance losses can be caused by particulate accretion, molecular film accretion, and impact cratering. A quantitative relationship is defined for film thickness and loss of throughput. Formulas are also developed for cases where induced surface defects are larger than the desired viewing wavelengths, or smaller or of the same order of the observed wavelengths. The techniques are used to quantify the degradation of a VUV solar coronagraph, a VUV stellar telescope, and a solar cell due to TIS. Applications are projected for estimating the contamination sensitivity of specific instruments, assessing the contamination hazard from known particulates, or to define clean room standards.

Bremer, J. C.↗
NASA NTRS1986

Correlation of Ultrasonic Polar Backscatter with the Deply Technique for Assessment of Impact Damage in Composite Laminates

Ultrasonic polar backscatter, a quantitative NDE technique introduced by O'Donnell and Miller (1981), was used to characterize impact damages in a 16-ply graphite-epoxy laminate. The results were correlated with the results obtained by the destructive deply technique of Freeman (1984). The size, shape, and orientation of damage correlated well between the polar backscatter technique and the deply technique. There was good quantitative correlation between the areas of damage indicated by the two techniques, suggesting that the polar backscatter technique is sensitive to specific orientations of damage. The polar backscatter technique provides a good qualitative image of the size and shape of the largest zone of damage in each of the principal orientations; a quantitative estimate of the extent of these largest damage zones can then be obtained.

Blodgett, E. D.↗
NASA NTRS1988

Infrared radiometric technique in temperature measurement

One class of commercially available imaging infrared radiometers using cooled detectors is sensitive to radiation over the 3 to 12 micron wavelength band. Spectral filters can tailor instrument sensitivity to specific regions where the target exhibits optimum radiance. The broadband spectral response coupled with real time two-dimensional imaging and emittance/background temperature corrections make the instruments useful for remote measurement of surface temperatures from -20 C to +1500 C. Commonly used radiometric techniques and assumptions are discussed, and performance specifications for a typical modern commercial instrument are presented. The potential usefulness of an imaging infrared radiometer in space laboratories is highlighted through examples of research, nondestructive evaluation, safety, and routine maintenance applications. Future improvements in instrument design and application of the radiometric technique are discussed.

Glazer, S.↗
NASA NTRS1986

Affinity immunoblotting - High resolution isoelectric focusing analysis of antibody clonotype distribution

A sensitive and specific method is proposed for the analysis of specific antibody clonotype changes occurring during an immune response and for comparing multiple sera for antibody clonotype similarities. Polyclonal serum antibodies separated by isoelectric focusing (IEF) were analyzed by an affinity immunoblotting method using antigen-coated nitrocellulose membranes. Antibodies present on the surface of the acrylamide gels following IEF bind the antigen on the nitrocellulose when the coated nitrocellulose is laid over the gels. The technique has been used to analyze Ig clonotypes specific for five protein antigens and two carbohydrate antigens. Optimal antigen concentrations for coating the nitrocellulose membranes were found to range from 10-100 microgram/ml.

Knisley, Keith A.↗
NASA NTRS1990

Measurement of trace stratospheric constituents with a balloon borne laser radar

The objective of this research was to measure the concentration of the stratospheric hydroxyl radical and related chemical species as a function of altitude, season, and time of day. Although hydroxyl plays a very important role in the chemistry controlling stratospheric ozone, little is known about its behavior because it has been a difficult species to measure. The instrument employed in this program was a laser radar, employing the technique of remote laser induced fluorescence. This instrument offers a number of attractive features including extreme specificity and sensitivity, a straightforward relationship between observed quantity and the desired concentration, and immunity to self-contamination.

Heaps, William S.↗