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At least 109 records · Page 6

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DOE OSTI2025

Role of Ribosomal Protein bS1 in Orthogonal mRNA Start Codon Selection

In many bacteria, the location of the mRNA start codon is determined by a short ribosome binding site sequence that base pairs with the 3'-end of 16S rRNA (rRNA) in the 30S subunit. Many groups have changed these short sequences, termed the Shine-Dalgarno (SD) sequence in the mRNA and the anti-Shine-Dalgarno (ASD) sequence in 16S rRNA, to create "orthogonal" ribosomes to enable the synthesis of orthogonal polymers in the presence of the endogenous translation machinery. However, orthogonal ribosomes are prone to SD-independent translation. Ribosomal protein bS1, which binds to the 30S ribosomal subunit, is thought to promote translation initiation by shuttling the mRNA to the ribosome. Thus, a better understanding of how the SD and bS1 contribute to start codon selection could help efforts to improve the orthogonality of ribosomes. Here, we engineered the Escherichia coli ribosome to prevent binding of bS1 to the 30S subunit and separate the activity of bS1 binding to the ribosome from the role of the mRNA SD sequence in start codon selection. We find that ribosomes lacking bS1 are slightly less active than wild-type ribosomes in vitro. Furthermore, orthogonal 30S subunits lacking bS1 do not have an improved orthogonality. Our findings suggest that mRNA features outside the SD sequence and independent of binding of bS1 to the ribosome likely contribute to start codon selection and the lack of orthogonality of present orthogonal ribosomes.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2022

An optimally tuned range-separated hybrid starting point for ab initio GW plus Bethe–Salpeter equation calculations of molecules

The ab initio GW plus Bethe–Salpeter equation (GW-BSE, where G is the one particle Green's function and W is the screened Coulomb interaction) approach has emerged as a leading method for predicting excitations in both solids and molecules with a predictive power contingent upon several factors. Among these factors are the (1) generalized Kohn–Sham eigensystem used to construct the GW self-energy and to solve the BSE and (2) the efficacy and suitability of the Tamm–Dancoff approximation. Here, we present a detailed benchmark study of low-lying singlet excitations from a generalized Kohn–Sham (gKS) starting point based on an optimally tuned range-separated hybrid (OTRSH) functional. We show that the use of this gKS starting point with one-shot G0W0 and G0W0-BSE leads to the lowest mean absolute errors (MAEs) and mean signed errors (MSEs), with respect to high-accuracy reference values, demonstrated in the literature thus far for the ionization potentials of the GW100 benchmark set and for low-lying neutral excitations of Thiel’s set molecules in the gas phase, without the need for self-consistency. The MSEs and MAEs of one-shot G0W0-BSE@OTRSH excitation energies are comparable to or lower than those obtained with other functional starting points after self-consistency. Additionally, we compare these results with linear-response time-dependent density functional theory (TDDFT) calculations and find GW-BSE to be superior to TDDFT when calculations are based on the same exchange-correlation functional. This work demonstrates tuned range-separated hybrids used in combination with GW and GW-BSE can greatly suppress starting point dependence for molecules, leading to accuracy similar to that for higher-order wavefunction-based theories for molecules without the need for costlier iterations to self-consistency.

McKeon, Caroline A. (ORCID:0000000217373503)↗

DOE OSTI2021

Multiple Grid-Forming Inverters in Black-Start: The Challenges

Black-start is a major challenge toward the successful operation of an islanded inverter-based system. In this article, the technical challenges for the black-start of multiple grid-forming inverters are reported. Specifically, this article discusses inverter synchronization, voltage and frequency regulation, and power-sharing between the grid-forming inverters. Experiment results from a multi-inverter test-bed have been provided to support the discussion. Furthermore, a grid-forming inverter controller for black-start operation is developed and presented in this article that addresses the discussed technical challenges. The performance of the developed controller in black-start operation is verified through experiment results.

Sadeque, Fahmid↗

DOE OSTI2016

SU-F-T-414: Mathematical Formulation of Gantry Starting Angle for Right Medial Tangential Arc in Left Intact Partial Breast Irradiation Using Volumetric Modulated Arc Therapy (VMAT)

Purpose: To choose appropriate gantry starting angle for partial left breast irradiation using volumetric modulated arc therapy (VMAT). Methods: A random patient of left breast carcinoma was selected for this study. The slice which was selected for this mathematical formulation was having maximum breast thickness and maximum medial and lateral tangential distance. After this appropriate isocenter was chosen on that CT slice. The distances between various points were measured by the measuring tool in Monaco 5.00.04. Using the various trigonometric equations, a final equation was derived which shows the relationship between Gantry start angle, isocenter Location and tissue thickness. Results: The final equation for gantry start for right medial tangential arc is given asStarting angle = 270°+tan^(−1)(sin(θ)/(x-1/x-2 +cosθ))The above equation was tested for 10 cases and it was found to be appropriate for all the cases. Conclusion: Gantry starting angle for partial arc irradiation depends upon Breast thickness, Distance between Medial and lateral tangent and isocenter location.

60 APPLIED LIFE SCIENCES↗

DOE OSTI2012

The Physics of Tokamak Start-up

Tokamak start-up on present-day devices usually relies on inductively induced voltage from a central solenoid. In some cases inductive startup is assisted with auxiliary power from electron cyclotron radio frequency heating. ITER, the National Spherical Torus eXperiment Upgrade and JT60, now under construction, will make use of the understanding gained from present-day devices to ensure successful start-up. Design of a spherical tokamak (ST) with DT capability for nuclear component testing would require an alternative to a central solenoid because the small central column in an ST has insufficient space to provide shielding for the insulators in the solenoid. Alternative start-up techniques such as induction using outer poloidal field coils, electron Bernstein wave start-up, coaxial helicity injection and point source helicity injection have been used with success, but require demonstration of scaling to higher plasma current.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

NASA NTRS1956

Turbojet-engine Starting and Acceleration

From considerations of safety and reliability in performance of gas-turbine aircraft, it is clear that engine starting and acceleration are of utmost importance. For this reason extensive efforts have been devoted to the investigation of the factors involved in the starting and acceleration of engines. In chapter III it is shown that certain basic combustion requirements must be met before ignition can occur; consequently, the design and operation of an engine must be tailored to provide these basic requirements in the combustion zone of the engine, particularly in the vicinity of the ignition source. It is pointed out in chapter III that ignition by electrical discharges is aided by high pressure, high temperature, low gas velocity and turbulence, gaseous fuel-air mixture, proper mixture strength, and-an optimum spark. duration. The simultaneous achievement of all these requirements in an actual turbojet-engine combustor is obviously impossible, yet any attempt to satisfy as many requirements as possible will result in lower ignition energies, lower-weight ignition systems, and greater reliability. These factors together with size and cost considerations determine the acceptability of the final ignition system. It is further shown in chapter III that the problem of wall quenching affects engine starting. For example, the dimensions of the volume to be burned must be larger than the quenching distance at the lowest pressure and the most adverse fuel-air ratio encountered. This fact affects the design of cross-fire tubes between adjacent combustion chambers in a tubular-combustor turbojet engine. Only two chambers in these engines contain spark plugs; therefore, the flame must propagate through small connecting tubes between the chambers. The quenching studies indicate that if the cross-fire tubes are too narrow the flame will not propagate from one chamber to another. In order to better understand the role of the basic factors in actual engine operation, many investigations have been conducted in single combustors from gas-turbine engines and in full-scale engines in altitude tanks and in flight. The purpose of the present chapter is to discuss the results of such studies and, where possible, to interpret these results qualitatively in terms of the basic requirements reported in chapter III. The discussion parallels the three phases of turbojet engine starting: (1) Ignition of the fuel-air mixture (2) Propagation of flame throughout the combustion zone (3) Acceleration of the engine to operating speed.

Mc Cafferty, R. J.↗

NASA NTRS1982

Comparison of starting values for iterative solutions to a universal Kepler's equation

General starting values for the iterative numerical solution of a universal Kepler's equation for position in a conic orbit at a specified time are investigated. Three starting values based on recent refinements of previously obtained bounds on the solution are derived and tested numerically. Of these, a simple starting value based on a cubic approximation to Kepler's equation provides the most rapid convergence using both first and second order Newton algorithms. The performance of the starting values are compared with similar studies which used the restricted case of elliptical orbits with the initial epoch at periapse.

Bergam, M. J.↗

NASA NTRS1989

Mathematical modeling and analysis of heat pipe start-up from the frozen state

The start-up process of a frozen heat pipe is described and a complete mathematical model for the start-up of the frozen heat pipe is developed based on the existing experimental data, which is simplified and solved numerically. The two-dimensional transient model for the wall and wick is coupled with the one-dimensional transient model for the vapor flow when vaporization and condensation occur at the interface. A parametric study is performed to examine the effect of the boundary specification at the surface of the outer wall on the successful start-up from the frozen state. For successful start-up, the boundary specification at the outer wall surface must melt the working substance in the condenser before dry-out takes place in the evaporator.

Jang, Jong Hoon↗

NASA NTRS1990

Mathematical modeling and analysis of heat pipe start-up from the frozen state

Jang, J. H.↗

NASA NTRS1990

Cool-down and frozen start-up behavior of a grooved water heat pipe

A grooved water heat pipe was tested to study its characteristics during the cool-down and start-up periods. The water heat pipe was cooled down from the ambient temperature to below the freezing temperature of water. During the cool-down, isothermal conditions were maintained at the evaporator and adiabatic sections until the working fluid was frozen. When water was frozen along the entire heat pipe, the heat pipe was rendered inactive. The start-up of the heat pipe from this state was studied under several different operating conditions. The results show the existence of large temperature gradients between the evaporator and the condenser, and the moving of the melting front of the working fluid along the heat pipe. Successful start-up was achieved for some test cases using partial gravity assist. The start-up behavior depended largely on the operating conditions.

Jang, Jong Hoon↗

NASA NTRS1993

Engine management during NTRE start up

The topics are presented in viewgraph form and include the following: total engine system management critical to successful nuclear thermal rocket engine (NTRE) start up; NERVA type engine start windows; reactor power control; heterogeneous reactor cooling; propellant feed system dynamics; integrated NTRE start sequence; moderator cooling loop and efficient NTRE starting; analytical simulation and low risk engine development; accurate simulation through dynamic coupling of physical processes; and integrated NTRE and mission performance.

Bulman, Mel↗

NASA NTRS1992

Investigation of starting transients in the thermally choked ram accelerator

An experimental investigation of the starting transients of the thermally choked ram accelerator is presented in this paper. Construction of a highly instrumented tube section and instrumentation inserts provide high resolution experimental pressure, luminosity, and electromagnetic data of the starting transients. Data obtained prior to and following the entrance diaphragm show detailed development of shock systems in both combustible and inert mixtures. With an evacuated launch tube, starting the diffuser is possible at any Mach number above the Kantrowitz Mach number. The detrimental effects and possible solutions of higher launch tube pressures and excessive obturator leakage (blow-by) are discussed. Ignition of a combustible mixture is demonstrated with both perforated and solid obturators. The relative advantages and disadvantages of each are discussed. Data obtained from these starting experiments enhance the understanding of the ram accelerator, as well as assist in the validation of unsteady, chemically reacting CFD codes.

Burnham, E. A.↗

NASA NTRS2001

Photoluminescence Studies of ZnSe Starting Materials and Vapor Grown Bulk Crystals

Low-temperature photoluminescence (PL) spectra were measured on ZnSe starting materials provided by various vendors and on bulk crystals grown from these starting materials by physical vapor transport (PVT) to study the effects of purification and contamination during PVT process. The impurity levels in one set of starting material/grown crystal were also measured by glow discharge mass spectroscopy (GDMS). The purification effect of pre-growth heat treatments and the PVT process is evidenced from the GDMS results which showed orders of magnitude reduction in the Li and Na concentration and a factor of 3 reduction in the O content after growth. The PL spectra showed that the strong emissions associated with Li (or Na) in one of the starting materials disappeared after growth. The GDMS results also showed increases in the At and Si contents by orders of magnitude after growth. To evaluate the contamination of the crystal during the high temperature growth process, three growth runs were processed using similar growth parameters but different furnace environments. The PL spectra suggest that the At contamination originated from the fused silica ampoule and that the Inconel cartridge might have been the cause of the broad Cu green and Cu red bands observed in one of the grown crystals.

Su, Ching-Hua↗

NASA NTRS2006

Feasibility Study of Jupiter Icy Moons Orbiter Permanent Magnet Alternator Start Sequence

The Jupiter Icy Moons Orbiter (JIMO) mission was a proposed, (recently cancelled) long duration science mission to study three moons of Jupiter: Callisto, Ganymede, and Europa. One design of the JIMO spacecraft used a nuclear heat source in conjunction with a Brayton rotating machine to generate electrical power for the electric thrusters and the spacecraft bus. The basic operation of the closed cycle Brayton system was as follows. The working fluid, a heliumxenon gas mixture, first entered a compressor, then went through a recuperator and hot-side heat exchanger, then expanded across a turbine that drove an alternator, then entered the cold-side of the recuperator and heat exchanger and finally returned to the compressor. The spacecraft was to be launched with the Brayton system off-line and the nuclear reactor shut down. Once the system was started, the helium-xenon gas would be circulated into the heat exchangers as the nuclear reactors were activated. Initially, the alternator unit would operate as a motor so as to drive the turbine and compressor to get the cycle started. This report investigated the feasibility of the start up sequence of a permanent magnet (PM) machine, similar in operation to the alternator unit, without any position or speed feedback sensors ("sensorless") and with a variable load torque. It is found that the permanent magnet machine can start with sensorless control and a load torque of up to 30 percent of the rated value.

Kenny, Barbara H.↗

NASA NTRS1951

Factors Affecting the Starting Characteristics of Gas-Turbine Engines

This report summarizes the effects of fuel volatility and engine design variables on the problem of starting gas-turbine engines at sea-level and altitude conditions. The starting operation for engines with tubular combustors is considered as three steps; namely, (1) ignition of a fuel-air mixture in the combustor, (2) propagation of flame through cross-fire tubes to all combustors, and (3) acceleration of the engine from windmilling or starting speed to the operating speed range. Pertinent data from laboratory researches, single-combustor studies, and full-scale engine investigations are presented on each phase of the starting problem.

Source record↗

DOE OSTI2012

Acceleration of Monte Carlo Criticality Calculations Using Deterministic-Based Starting Sources

A new automatic approach that uses approximate deterministic solutions for providing the starting fission source for Monte Carlo eigenvalue calculations was evaluated in this analysis. By accelerating the Monte Carlo source convergence and decreasing the number of cycles that has to be skipped before the tally's estimation, this approach was found to increase the efficiency of the overall simulation, even with the inclusion of the extra computational time required by the deterministic calculation. This approach was also found to increase the reliability of the Monte Carlo criticality calculations of loosely coupled systems because the use of the better starting source reduces the likelihood of producing an undersampled k eff due to the inadequate source convergence. The efficiency improvement was demonstrated using two of the standard test problems devised by the OECD/NEA Expert Group on Source Convergence in Criticality-Safety Analysis to measure the source convergence in Monte Carlo criticality calculations. For a fixed uncertainty objective, this approach increased the efficiency of the overall simulation by factors between 1.2 and 3 depending on the difficulty of the source convergence in these problems. The reliability improvement was demonstrated in a modified version of the 'k eff of the world' problem that was specifically designed to demonstrate the limitations of the current Monte Carlo power iteration techniques. For this problem, the probability of obtaining a clearly undersampled k eff decreased from 5% with a uniform starting source to zero with a deterministic starting source when batch sizes with more than 15,000 neutron/cycle were used.

42 ENGINEERING↗

DOE OSTI2015

Site selection in Germany - Start of the evaluation of the site selection law - 15561

Since 2011 Germany is pursuing a phase out strategy concerning the use of nuclear power for electricity production. This decision was strongly influenced by the Fukushima event. In 2013 the federal government announced that they also had achieved an agreement with the Federal States in Germany on a law to restart the site selection for a repository for spent fuel and high active heat producing waste from scratch. The consequence of this law is a delay of at least two decades to start operation of a final disposal site and additional costs of at least EUR 2.7 billion. The new law was passed in July 2013. At first a 34-member commission had been installed in April 2014 to evaluate the Site Selection Law and to develop basic principles for site selection, including safety requirements and selection criteria for rock formations. The commission includes representatives from the parliament, academia, civil society organizations, industry, the environmental organizations and trade unions and should forward its recommendations after a delay in starting the evaluation process now in 2016. The first author of this paper is member of the evaluation commission. The present law has to be reviewed within this time span. The site selection then might start after the next federal election in 2017 probably based on a new site selection law. This new site selection law will take into account or even follow the recommendations of the evaluation group. A new repository site should be according to the present law determined till 2031 and for this site the more detailed site investigation will take place followed by a detailed safety analysis, before the erection of the repository can start. Based on the present procedural steps it seems to be rather unlikely to determine a repository site till 2031. As shown in this publication, there will be a delay of at least 20 years compared to the schedule given in the site selection law until a repository site can be determined. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

DOE OSTI2026

Influence of Operating Conditions on Ethanol Passive Prechamber Cold-start Operation

Cold-start operation in spark ignition engines is characterized by many drawbacks such as poor fuel vaporization, substantial wall film formation, and weak ignition which can result in unstable combustion and high concentrations of carbon monoxide (CO), nitrogen oxides (NOx), and unburnt hydrocarbons (UHC) prior to catalyst light-off. These challenges are particularly important for ethanol as the fuel’s high latent heat of vaporization and low vapor pressure can hinder reliable ignition during cold-start. In this context, passive prechamber ignition systems offer a viable pathway to improve ignitability by producing hot turbulent jets that can promote combustion in the main chamber. In this study, the influence of operating conditions on jet formation and combustion characteristics in an ethanol-fueled passive prechamber spark ignition engine is investigated. Two intake pressures (40 kPa and 60 kPa) and two engine speeds (450 and 600 rpm) are studied to represent various stages of the cold-start ramp-up. Results indicate that lower intake pressures lead to delayed and asymmetric turbulent jet formation, and lower peak heat release rates. Lower engine speeds, on the other hand, produced higher peak heat release and faster combustion due to greater residence time in the engine. This greater residence time also increases the likelihood of autoignition in the main chamber, potentially promoting quicker heat release for lower engine speeds. These results provide insight into the role of operating conditions on cold-start ramp-up of passive prechamber engines.

Banagiri, Shrikar [ORNL] (ORCID:0000000239745099)↗