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At least 109 records · Page 6

Mechanical stimulation of skeletal muscle generates lipid-related second messengers by phospholipase activation

Repetitive mechanical stimulation of cultured avian skeletal muscle increases the synthesis of prostaglandins E2 and F2(alpha) which regulate protein turnover rates and muscle cell growth. Mechnical stimulation significantly increases the breakdown rate of (3)H-arachidonic acid labelled phospholipids, releasing free (3)H-arachidonic acid, and the rate-limiting precursor of prostaglandin synthesis. Mechanical stimulation also significantly increases (3)H-arachidonic acid labelled diacylglycerol formation and intracellular levels of inositol phosphates from myo-2-(3)H inositol labelled phospholipids. Phospholipase A2, phosphatidylinositol-specific phospholipase C (PLC), and phospholipase D (PLD) are activated by stretch. The lipase inhibitors bromophenacylbromide and RHC80267 together reduce stretch-induced prostaglandin production by 73-83 percent. The stretch-induced increases in prostaglandin production, (3)H-arachidonic acid labelled phospholipid breakdown, and (3)H-arachidonic acid labelled diacylglycerol formation occur independently of cellular electrical activity (tetrodotoxin insensitive) whereas the formation of inositol phosphates from myo-2-(3)H inositol labelled phospholipids are dependent on cellular electrical activity. These results indicate that mechanical stimulation increases the lipid-related second messengers arachidonic acid, diacylglycerol, and prostaglandins through activation of specific phospholipases such as PLA2 and PLD, but not by activation of phosphatidylinositol-specific PLC.

Vandenburgh, Herman H.↗

New calibrators for the Cepheid period-luminosity relation

IUE spectra of six Cepheids have been used to determine their absolute magnitudes from the spectral types of their binary companions. The stars observed are U Aql, V659 Cen, Y Lac, S Nor, V350 Sgr, and V636 Sco. The absolute magnitude for V659 Cen is more uncertain than for the others because its reddening is poorly determined and the spectral type is hotter than those of the others. In addition, a reddening law with extra absorption in the 2200 A region is necessary, although this has a negligible effect on the absolute magnitude. For the other Cepheids, and also Eta Aql and W Sgr, the standard deviation from the Feast and Walker period-luminosity-color (PLC) relation is 0.37 mag, confirming the previously estimated internal uncertainty. The absolute magnitudes for S Nor from the binary companion and from cluster membership are very similar. The preliminary PLC zero point is less than 2 sigma (+0.21 mag) different from that of Feast and Walker. The same narrowing of the instability strip at low luminosities found by Fernie is seen.

Evans, Nancy R.↗

Effect of insulin-like factors on glucose transport activity in unweighted rat skeletal muscle

The effect of 3 or 6 days of unweighting on glucose transport activity, as assessed by 2-deoxyglucose uptake, in soleus strips stimulated by maximally effective concentrations of insulin, IGF-I, vanadate, or phospholipase C (PLC) is examined. Progressively increased responses to maximally effective doses of insulin or insulin-like growth factor were observed after 3 and 6 days of unweighting compared with weight matched control strips. Enhanced maximal responses to vanadate (6 days only) and PLC (3 and 6 days) were also observed. The data provide support for the existance of postreceptor binding mechanisms for the increased action of insulin on the glucose transport system in unweighted rat skeletal muscle.

Henriksen, Erik J.↗

Refurbishment and Automation of the Thermal/Vacuum Facilities at the Goddard Space Flight Center

The thermal/vacuum facilities located at the Goddard Space Flight Center (GSFC) have supported both manned and unmanned space flight since the 1960s. Of the 11 facilities, currently 10 of the systems are scheduled for refurbishment and/or replacement as part of a 5-year implementation. Expected return on investment includes the reduction in test schedules, improvements in the safety of facility operations, reduction in the complexity of a test and the reduction in personnel support required for a test. Additionally, GSFC will become a global resource renowned for expertise in thermal engineering, mechanical engineering and for the automation of thermal/vacuum facilities and thermal/vacuum tests. Automation of the thermal/vacuum facilities includes the utilization of Programmable Logic Controllers (PLCs) and the use of Supervisory Control and Data Acquisition (SCADA) systems. These components allow the computer control and automation of mechanical components such as valves and pumps. In some cases, the chamber and chamber shroud require complete replacement while others require only mechanical component retrofit or replacement. The project of refurbishment and automation began in 1996 and has resulted in the computer control of one Facility (Facility #225) and the integration of electronically controlled devices and PLCs within several other facilities. Facility 225 has been successfully controlled by PLC and SCADA for over one year. Insignificant anomalies have occurred and were resolved with minimal impact to testing and operations. The amount of work remaining to be performed will occur over the next four to five years. Fiscal year 1998 includes the complete refurbishment of one facility, computer control of the thermal systems in two facilities, implementation of SCADA and PLC systems to support multiple facilities and the implementation of a Database server to allow efficient test management and data analysis.

Donohue, John T.↗

A System for Managing Replenishment of a Nutrient Solution Using an Electrical Conductivity Controller

Control of nutrient solution parameters is very important for the growth and development of plants grown hydroponically. Protocols involving different nutrient solution replenishment times (e.g. one-week, two-week, or two-day replenishment) provide manual periodic control of the nutrient solution's electrical conductivity (EC). Since plants take-up nutrients as they grow, manual control has a drawback in that EC is not held constant between replenishments. In an effort to correct this problem the Center for Food and Environmental Systems for Human Exploration of Space at Tuskegee University has developed a system for managing and controlling levels of EC over a plant's entire growing cycle. A prototype system is being tested on sweetpotato production using the nutrient film technique (NFT), and it is being compared to a system in which sweetpotatoes are grown using NFT with manual control. NASA has played an important role in the development of environmental control systems. They have become a forerunner in growing plants hydroponically with some control systems through the use of networked data acquisition and control using environmental growth chambers. Data acquisition systems which involve the use of real-time, calibration, set points, user panel, and graphical representation programming provide a good method of controlling nutrient solution parameters such as EC and pH [Bledsoe, 19931]. In NASA's Biomass Production Chamber (BPC) at Kennedy Space Center, control is provided by a programmable logic controller (PLC). This is an industrial controller which combines ladder computer logic which has the ability to handle various levels of electrical power. The controller controls temperature, light and other parameters that affect the plant's environment, in the BPC, the Nutrient Delivery System (NIX), a sub-system of the PLC, controls nutrient solution parameters such as EC, pH, and solution levels. When the nutrient EC measurement goes outside a preset range (120-130 mS/m) a set amount of a stock solution of nutrients is automatically added by a metering pump to bring the EC back into operating range [Fortson, 1992]. This paper describes a system developed at Tuskegee University for controlling the EC of a nutrient solution used for growing sweetpotatoes with an EC controller and a computer with LabView data acquisition and instrumentation software. It also describes the preliminary data obtained from the growth of sweetpotatoes using this prototype control system.

Davis, D.↗

NASA Ames Research Center 60 MW Power Supply Modernization

The NASA Ames Research Center 60 MW DC Power Supply was built in 1974 to provide controlled DC power for the Thermophysics Facility Arc Jet Laboratory. The Power Supply has gradually losing reliability due to outdated technology and component life limitation. NASA has decided to upgrade the existing rectifier modules with contemporary high-power electronics and control equipment. NASA plans to complete this project in 2001. This project includes a complete replacement of obsolete thyristor stacks in all six rectifier modules and rectifier bridge control system. High power water-cooled thyristors and freewheeling diodes will be used. The rating of each of the six modules will be 4000 A at 5500 V. The control firing angle signal will be sent from the Facility Control System to six modules via fiberoptic cable. The Power Supply control and monitoring system will include a Master PLC in the Facility building and a Slave PLC in each rectifier module. This system will also monitor each thyristor level in each stack and the auxiliary equipment.

Choy, Yuen Ching↗

Assembly For Moving a Robotic Device Along Selected Axes

An assembly for moving a robotic device along selected axes includes a programmable logic controller (PLC) for controlling movement of the device along selected axes to effect movement of the device to a selected disposition. The PLC includes a plurality of single axis motion control modules, and a central processing unit (CPU) in communication with the motion control modules. A human-machine interface is provided for operator selection of configurations of device movements and is in communication with the CPU. A motor drive is in communication with each of the motion control modules and is operable to effect movement of the device along the selected axes to obtain movement of the device to the selected disposition.

Brentley Craig Nowlin↗

Mechanical stimulation of skeletal muscle generates lipid-related second messengers by phospholipase activation

Repetitive mechanical stimulation of cultured avian skeletal muscle increases the synthesis of prostaglandins (PG) E2 and F2 alpha which regulate protein turnover rates and muscle cell growth. These stretch-induced PG increases are reduced in low extracellular calcium medium and by specific phospholipase inhibitors. Mechanical stimulation increases the breakdown rate of 3H-arachidonic acid labelled phospholipids, releasing free 3H-arachidonic acid, the rate-limiting precursor of PG synthesis. Mechanical stimulation also increases 3H-arachidonic acid labelled diacylglycerol formation and intracellular levels of inositol phosphates from myo-[2-3H]inositol labelled phospholipids. Phospholipase A2 (PLA2), phosphatidylinositol-specific phospholipase C (PLC), and phospholipase D (PLD) are all activated by stretch. The stretch-induced increases in PG production, 3H-arachidonic acid labelled phospholipid breakdown, and 3H-arachidonic acid labelled diacylglycerol formation occur independently of cellular electrical activity (tetrodotoxin insensitive) whereas the formation of inositol phosphates from myo-[2-3H]inositol labelled phospholipids is dependent on cellular electrical activity. These results indicate that mechanical stimulation increases the lipid-related second messengers arachidonic acid, diacylglycerol, and PG through activation of specific phospholipases such as PLA2 and PLD, but not by activation of phosphatidylinositol-specific PLC.

NASA Discipline Number 40-40↗

NASA Glenn's Single-Stage Axial Compressor Facility Upgraded

NASA Glenn Research Center's Single-Stage Axial Compressor Facility was upgraded in fiscal year 2003 to expand and improve its research capabilities for testing high-speed fans and compressors. The old 3000-hp drive motor and gearbox were removed and replaced with a refurbished 7000-hp drive motor and gearbox, with a maximum output speed of 21,240 rpm. The higher horsepower rating permits testing of fans and compressors with higher pressure ratio or higher flow. A new inline torquemeter was installed to provide an alternate measurement of fan and compressor efficiency, along with the standard pressure and temperature measurements. A refurbished compressor bearing housing was also installed with bidirectional rotation capability, so that a variety of existing hardware could be tested. Four new lubrication modules with backup capability were installed for the motor, gearbox, torquemeter, and compressor bearing housing, so that in case the primary pump fails, the backup will prevent damage to the rotating hardware. The combustion air supply line for the facility inlet air system was activated to provide dry air for repeatable inlet conditions. New flow conditioning hardware was installed in the facility inlet plenum tank, which greatly reduced the inlet turbulence. The new inlet can also be easily modified to accommodate 20- or 22-in.-diameter fans and compressors, so a variety of existing hardware from other facilities (such as Glenn's 9- by 15-Foot Low-Speed Wind Tunnel) can be tested in the Single-Stage Axial Compressor Facility. An exhaust line was also installed to provide bleed capability to remove the inlet boundary layer. To improve the operation and control of the facility, a new programmable logic controller (PLC) was installed to upgrade from hardwired relay logic to software logic. The PLC also enabled the usage of human-machine interface software to allow for easier operation of the facility and easier reconfiguration of the facility controls when necessary. Finally, a new health-monitoring system was installed to measure shaft speed, shaft movement, and machine vibration on all of the rotating equipment. The system also provides automatic alarms and shutdown of the equipment when required. An integrated systems test for checkout of the facility was completed on June 17, 2003. Research testing resumed on June 30 with data obtained in support of the Ultra-Efficient Engine Technology Project's Compressor Casing Treatment Project.

Brokopp, Richard A.↗

Hardwired Control Changes For NSTX DC Power Feeds

The National Spherical Torus Experiment (NSTX) has been designed and installed in the existing facilities at Princeton Plasma Physics Laboratory (PPPL). Most of the hardware, plant facilities, auxiliary sub-systems, and power systems originally used for the Tokamak Fusion Test Reactor (TFTR) have been used with suitable modifications to reflect NSTX needs. The original TFTR Hardwired Control System (HCS) with electromechanical relays was used for NSTX DC Power loop control and protection during NSTX operations. As part of the NSTX Upgrade, the HCS is being changed to a PLC-based system with the same control logic. This paper gives a description of the changeover to the new PLC-based system __________________________________________________

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

In-Containment Signal Acquisition and Data Transmission via Power Lines within High Dose Areas of Nuclear Power Plants

Signal acquisition and data transmission for innovative sensor systems and networks inside the containment of nuclear power plants (NPPs) is still a challenge with respect to safety, performance, reliability, availability, and costs. This especially applies to equipment upgrades for existing plants, special measurements, but also for new builds. This paper presents a novel method for efficient and cost-effective sensor signal acquisition and data transmission via power lines, in order to cope with the disadvantages of common system architectures that often suffer from poor signal integrity due to raw data transmissions via long cables, huge efforts and costs for installation, and low flexibility with respect to maintenance and upgrades. A transmitter-receiver architecture is proposed that allows multiplexing of multiple sensor inputs for unidirectional point-to-point transmission by superimposing information signals on existing AC or DC supply lines, but also on active and inactive sensor wires, or spare cables, using power line communication (PLC) technology. Based on commercial off-the-shelf (COTS) electronic parts, a radiation hard transmitter hardware is designed to operate in harsh environment within the containment during full plant operation. The system's modular approach allows application specific trade-offs between redundancy and throughput regarding data transmission, as well as various sensor input front-ends which are compatible with state of the art systems. PLC technology eliminates the need for costly installation of additional cables and wall penetrations, while providing a complementary and diverse communication technology for upgrades of existing systems. At the receiver side in low dose areas, signals are extracted from the power line, demodulated, and de-multiplexed, in order to regain the original sensor signal information and provide it either in analog or digital output format. Successful laboratory qualification tests, field trails, and a demonstrator setup installation within a NPP verify the overall performance of the proposed system. (authors)

22 GENERAL STUDIES OF NUCLEAR REACTORS↗

Flexible Packaging and Real Time Assay Techniques to Divert Low Activity Waste LLW from the UK LLWR - 18011

Historically, all Low Level Waste (LLW) produced in the UK was sent to the Low Level Waste Repository (LLWR) near Drigg in Cumbria in mild steel IP2 containers. However, this single national asset is filling up very quickly and the UK national LLW policy is to divert waste to alternative sites wherever possible. This paper will describe how through a collaboration between Magnox Ltd, NUVIA Limited, Pactec EPS and Augean Plc has allowed for low activity LLW (<200 MBq/Tonne) to be packaged into IP1 flexible bags, assayed within the flexible packages and then consigned to a suitably designed and licensed landfill site. The Magnox site at Harwell in Oxfordshire is in the process of total decommissioning. A project was initiated to explore the viability of utilising the PACTEC LIFTPAC{sup R} IP1 flexible packages which can contain 1 m{sup 3} of waste and assaying the waste using the NUVIA Limited transportable High Resolution Assay Monitor (HiRAM) before sending the waste for disposal at the Augean Plc East Northants Resource Management Facility (ENRMF) in Cambridgeshire. The project led to a fundamental change in the culture of LLW disposal and the paper will explore the commonly held misconception that flexible packaging can only be used for the transportation and disposal of Very Low Level Waste (VLLW). It will also explore how the NUVIA Limited HiRAM system can assay large quantities of these bags in a short time period to a high level of accuracy such that they were able to consign the waste for disposal to ENRMF. This technique and disposal route is open and available to many other waste producers both within the UK and beyond and has proven cost savings to the waste producer. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Implementing a control application on an FPGA Platform - 356

Today, many I and C system suppliers propose FPGA-based I and C platforms. In that context, EDF has set up a project to assess the use of such platforms for safety-related systems and to enhance EDF's ability to manage this technology for the upgrade of existing I and C systems or for new constructions. One aim of the project is to assess the adequacy of these platforms for the development of control functions. This paper presents the development of a case study on a Radiy FPGA-based platform in a Hardware-In-the-Loop configuration, and the comparison with a Dymola Model-In-the-Loop and a Siemens T2000 S7 Hardware-In-the-Loop approach. The case study is a part of the level control of steam generators of French PWR NPP. The control flow diagram uses complex analog elementary blocks coded with fixed point format for the analog values. The use of IP cores of the FPGA supplier was avoided to enable a complete mastership of the VHDL code, to provide evidence of compliance with safety-related requirements, and to allow porting this code to another FPGA platform. The case study has highlighted the specificity of using a FPGA, and has shown that a complex control application in a FPGA-based PLC presents no major differences in precision and response time with a processor-based PLC. (authors)

21 SPECIFIC NUCLEAR REACTORS AND ASSOCIATED PLANTS↗

Development and application of FPGA-based logic controller - 357

The control systems of nuclear power plants contain many components which are becoming obsolete at an increasing rate. Nowadays maintenance for analog-based protection systems may be difficult as analog parts become obsolete or hard to obtain. Various studies have been conducted to address this control system hardware obsolescence issue. Those control systems of nuclear power plants have been replaced with modern digital control systems such as the programmable logic controller (PLC) and the distributed control system (DCS). The central processing unit (CPU)- based platforms such as the PLC and the DCS require an operating system (OS) and application software, and thus they might result in a common-cause failure when a problem occurs in the operating system or application software. The field programmable gate array (FPGA) is highlighted as an alternative to the conventional protection or control systems. This paper presents the development of an FPGA-based logic controller (FLC) and a diverse protection system (DPS) with the FLC platform. Protection functions for the DPS were implemented in FPGAs without any CPU or OS. (authors)

22 GENERAL STUDIES OF NUCLEAR REACTORS↗

Hardware-Based Demonstration of Temperature Control Functions for Reactor Systems

Establishing autonomy in reactor control systems has become essential for the expansion of nuclear technologies. Thermal regulation in particular remains crucial for maintaining stable operation and ensuring the integrity of fuel. To alleviate public skepticism of the safety of nuclear reactors, demonstrating control over this key factor is pivotal. Utilizing electric heat pads to simulate the heat released in a reactor core, thermocouples for temperature monitoring, and an Arduino micro programmable logic controller (PLC) for control, a hardware-based demonstration of a reactor heating system validates the efficacy of reactor control over this key parameter. To improve precision, a proportional-integral-derivative (PID) algorithm was implemented in the heating control loop to ensure meticulous control of reactor functions. In addition, the integration of this physical system with a digital simulator tool such as RELAP5-3D establishes a foundation for a comprehensive testing environment. This allows for a refinement of temperature control under various simulated reactor conditions, bringing another layer of reliability to the operation of the system. By facilitating a physical demonstration of reactor thermal management and control strategies, this project provides a foundation for expanded testing and educational outreach. Ultimately, this system advances the broader goal of demonstrating the safety and viability of autonomous reactor operations, contributing to public trust and future reactor deployment.

22 GENERAL STUDIES OF NUCLEAR REACTORS↗

Project Presentation: Hardware-Based Demonstration of Temperature Control Functions for Reactor Systems

Autonomous thermal regulation in nuclear reactors remains crucial for maintaining stable operation and ensuring the integrity of fuel. To alleviate public skepticism of the safety of nuclear reactors, demonstrating control over this key factor is pivotal. Utilizing electric heat pads to simulate the heat released in a reactor core, thermocouples for temperature monitoring, and an Arduino micro programmable logic controller (PLC) with an embedded proportional-integral-derivative (PID) algorithm for control, a hardware-based demonstration of a reactor heating system will validate the efficacy of reactor control over this key parameter. This report covers internship project presentation as well as relevant experience with nuclear and proposal for project upgrade.

21 SPECIFIC NUCLEAR REACTORS AND ASSOCIATED PLANTS↗

Control System Upgrade for Canal ARC SAW

Upgrading the specific components of the Arc Saw, such as the HMI, the components of the chassis involving the I/O modules, servo motor modules, and PLC modules offers numerous advantages. By enhancing the HMI, the operators will benefit having a user-friendly interface, enabling smoother control and monitoring of the ARC SAW’s functionality. Upgrading the modules will contribute to an improved precision and responsiveness in changing modes of the ARC SAW as well as the adjusting the saw blades position, further enhancing the system's overall performance. These upgrades, while maintaining the same functionality, enable the latest technological advancements without needing significant changes or disruptions to existing operations.

21 SPECIFIC NUCLEAR REACTORS AND ASSOCIATED PLANTS↗

ON THE EFFECTIVENESS OF LLMS IN UNIT TEST GENERATION FOR STRUCTURED TEXT PROGRAMS

The reliability of industrial automation systems heavily depends on the correctness of Programmable Logic Controller (PLC) programs, which are often written in Structured Text (ST). While Large Language Models (LLMs) have shown promise in automating test generation for mainstream programming languages, their effectiveness for the syntactically strict ST language remains underexplored. This thesis presents a systematic empirical evaluation of three state-of-the-art LLMs—GPT-4o, Gemini 2.5 Pro, and Claude Sonnet 4.5—for generating ST unit tests. We examine three prompting strategies: Natural Language (NL), Code Language (CL), and Chain-of-Thought (CoT), across a curated set of 11 ST function blocks. The quality of the generated tests is assessed using Compilation Success Rate (CSR), Statement Coverage (SC), and Branch Coverage (BC). In the zero-shot setting, Claude Sonnet 4.5 achieves the highest CSR, while Gemini 2.5 Pro consistently delivers the best statement and branch coverage, particularly under CL prompts. By incorporating a one-shot CL prompt, all models exhibit substantial improvements—most notably GPT-4o, whose CSR increases from 45.45% to 90.91%, with substantial gains in both SC and BC. To further contextualize these findings, we compare GPT-4o’s one-shot results with PLCAutoTester, a state-ofthe- art ST unit test generation tool, on an additional benchmark dataset. While LLMgenerated tests approach competitive coverage levels, PLCAutoTester maintains significantly higher and more stable coverage across programs. This study provides the first comprehensive benchmark of modern LLMs for ST unit testing, highlighting their strengths, limitations, and improvements through one-shot prompting, and positioning their performance relative to specialized automated testing tools in industrial automation.

42 ENGINEERING↗