Engineering Papers⌕ Search

SEARCH · Engineering Papers

Results for “Mars Science Lab”

Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

93 records · Page 6
All sourcesNASA NTRSDOE OSTI
NASA NTRS2000

Electrostatics of Granular Material (EGM): Space Station Experiment

Aggregates were observed to form very suddenly in a lab-contained dust cloud, transforming (within seconds) an opaque monodispersed cloud into a clear volume containing rapidly-settling, long hair-like aggregates. The implications of such a "phase change" led to a series of experiments progressing from the lab, to KC-135, followed by micro-g flights on USML-1 and USML-2, and now EGM slated for Space Station. We attribute the sudden "collapse" of a cloud to the effect of dipoles. This has significant ramifications for all types of cloud systems, and additionally implicates dipoles in the processes of cohesion and adhesion of granular matter. Notably, there is the inference that like-charged grains need not necessarily repel if they are close enough together: attraction or repulsion depends on intergranular distance (the dipole being more powerful at short range), and the D/M ratio for each grain, where D is the dipole moment and M is the net charge. We discovered that these ideas about dipoles, the likely pervasiveness of them in granular material, the significance of the D/M ratio, and the idea of mixed charges on individual grains resulting from tribological processes --are not universally recognized in electrostatics, granular material studies, and aerosol science, despite some early seminal work in the literature, and despite commercial applications of dipoles in such modern uses as "Krazy Glue", housecleaning dust cloths, and photocopying. The overarching goal of EGM is to empirically prove that (triboelectrically) charged dielectric grains of material have dipole moments that provide an "always attractive" intergranular force as a result of both positive and negative charges residing on the surfaces of individual grains. Microgravity is required for this experiment because sand grains can be suspended as a cloud for protracted periods, the grains are free to rotate to express their electrostatic character, and Coulombic forces are unmasked. Suspended grains will be "interrogated" by applied electrical fields. In one module, grains will be immersed in an inhomogeneous electric field and allowed to be attracted towards or repelled from the central electrode of the module: part of the grain's speed will be a function of its net charge (monopole), part will be a function of the dipole. Observed grain position vs. time will provide a curve that can be deconvolved into the dipole and monopole forces responsible, since both have distinctive radial dependencies. In a second approach, the inhomogeneous field will be alternated at low frequency (e.g., every 5-10 seconds) so that the grains are alternately attracted and repelled from the center of the field. The resulting "zigzag" grain motion will gradually drift inwards, then suddenly change to a unidirectional inward path when a critical radial distance is encountered (a sort of "Coulombic event horizon") at which the dipole strength supersedes the monopole strength --thus proving the presence of a dipole, while also quantifying the D/M ratio. In a second module, an homogeneous electric field eliminates dipole effects (both Coulombic and induced) to provide calibration of the monopole and to more readily evaluate net charge statistical variance. In both modules, the e-fields will be exponentially step-ramped in voltage during the experiment, so that the field "nominalizes" grain speed while spreading the response time --effectively forcing each grain to "wait its turn" to be measured. In addition to rigorously quantifying M, D, and the D/M ratio for many hundreds of grains, the experiment will also observe gross electrometric and RF discharge phenomena associated with grain activity. The parameter space will encompass grain charging levels (via intentional triboelectrification), grain size, cloud density, and material type. Results will prove or disprove the dipole hypothesis. In either case, light will be shed on the role of electrostatic forces in governing granular systems. Knowledge so gained can be applied to natural clouds such as protostellar and protoplanetary dust and debris systems, planetary rings, planetary dust palls and aerosols created by volcanic, impact, aeolian, firestorm, or nuclear winter processes. The data are also directly applicable to adhesion, cohesion, transport, dispersion, and collection of granular materials in industrial, agricultural, pharmaceutical applications, and in fields as diverse as dust contamination of space suits on Mars and crop spraying on Earth.

Marshall, J.↗
NASA NTRSDate not supplied

Does Collection Time Bias the Ecology of Cleanroom Air Samples?

Microbial monitoring of astromaterials collections has taken on increased importance with the return of biologically sensitive samples from the asteroids Ryugu and Bennu and the initiation of the Mars Sample Return Program. Terrestrial bacteria and fungi can alter the mineralogy and organic composition of our collections causing irreversible contamination of pristine samples and increasing the risk of false positives for life detection measurements. NASA has conducted routine microbial monitoring of its existing collections since 20181. Initial monitoring focused on surface samples collected with foam swabs. Although, airborne microbiology is often decoupled from surface microbiology in the built environment2 culture-based air sampling techniques like impactors were not compliant with existing contamination control requirements. Bringing organic rich media, gelatin or liquids into curation cleanrooms presents an unacceptable risk to pristine samples. In 2022 NASA purchased a materials complaint air sampler and began collecting air samples from the cleanrooms in addition to surface samples3. The new instrument uses an electret filter to collect samples that are suitable for cultivating organisms or for direct DNA sequencing. Preliminary DNA sequencing results appeared to indicate that longer sampling times biased the microbial community in favor of hearty, spore-forming bacteria3. We present the results of a study comparing overnight sampling (17 hours) to short (1 hour) sampling of unoccupied curation cleanrooms. The results will help us optimize our monitoring protocols and develop a more detailed inventory of the ecology of astromaterials curation cleanrooms. Methods: We analyzed 72 paired air samples from six different cleanrooms including the meteorite processing lab (ISO 7 equivalent, 16 samples), the lunar lab (ISO 6 equivalent, 10 samples), the stardust lab (ISO 5 equivalent 14 samples), the OSIRIS-REx lab (ISO 5 equivalent, 12 samples), the Hayabusa2 lab (ISO 5 equivalent, 14 samples), and the Genesis lab (ISO 4 equivalent, 6 samples). All the samples were collected with an InnovaPrep Bobcat air sampler operating at a sampling rate of 200 L/min. The sampler operates for 5 minutes out of every 20 minute period. Half of the samples were collected by filtering 3,000L (15 min. of active sampling) of air across an electret filter for one hour. The rest of the samples were collected by filtering approximately 51,000 L air across the filter overnight (~17 hours, 255 min. of active sampling). Cells were eluted from the filter using 6-7 ml of pressurized 0.15% tween 20 in PBS (phosphate buffered saline). This liquid was used to cultivate bacteria according to previously published methods1,4,5 and for DNA extraction and next generation sequencing. DNA was extracted with a Qiagen MagAttract PowerMicrobiome kit6. To identify bacteria and archaea, the 16S rRNA gene was amplified using Earth Microbiome primers for the V4 region 7. The amplified DNA was sequenced on an Illumina MiSeq using a V3 reagent kit. The resulting sequences were processed using DADA2 and QIIME2 as implemented on the EDGE bioinformatics platform8–10. Results: Only two of the 72 samples had no amplifiable DNA. Amplified DNA concentrations ranged from 2.67 – 0.272 ng/µl. The median concentration of amplified DNA for the 1 hour samples was 0.770 ± 0.368 ng/µl. The median concentration of amplified DNA for the overnight samples was 0.877 ± 0.434 ng/µl. On average the overnight samples had slightly more sequences (58,960 vs. 59,456) and ASV’s (amplicon sequence variants) (60 vs 64.5) than the one hour samples, but these differences are not statistically significant. The most abundant ASV in every sample mapped to the genus Cupravidus. ASV’s mapping to the genuses Bacillus, Schlegelella, Thermus, and Staphylococcus were also common. Discussion and Future Work: Alpha diversity statistics like Shannon Entropy and Faith Phylogenetic Diversity are used to describe the diversity of organisms in a single sample. If a longer sampling time was biasing the data, we would expect to see a change in these diversity statistics vs. sample time. However, we did not observe this in our data. The median Shannon entropy was slightly higher for the overnight samples (3.773 vs 3.611) as was the Faith Phylogenetic Diversity (4.042 vs 3.596), but both values were within a standard deviation of each other for the two sampling times (Fig. 1). It is unlikely, that the longer sampling time is introducing bias into our data. We do observe a significant decrease in diversity when comparing the air samples by lab. The Genesis lab (ISO 4 equivalent) has a lower median number of ASV’s (45.5) than the other labs (62). Median values for Shannon Entropy (3.717 vs. 3.430) and Faith Phylogenetic Diversity (3.796 vs. 3.548) are also lower for Genesis, but those values are with one standard deviation of each other for the different sampling times. This is consistent with previous culture-based results suggesting that the environment in cleanrooms tends to select for a core group of organisms capable of surviving under dry, low nutrient, conditions. The presence of the ASV’s mapping to Cupravidus and Thermus in our sequencing blanks and controls suggests that several of the most common organisms in our samples represent contaminants from the reagents used to perform the DNA extractions and sequencing. Further work is needed to identify these contaminants, remove them from our data and recalculate the diversity statistics. This is a systematic error. Therefore, we do not expect removing the sequencing contaminants to change our conclusions. Longer air sample collection times appear to result in slightly higher diversity and do not bias the results towards “hardy” bacteria like spore-formers. Based on these preliminary results we conclude that sampling at least 3,000 liters of air is sufficient to capture the microbial diversity of cleanrooms, and that air samples can also be collected overnight without negatively impacting diversity. These results allow us to be flexible when designing microbial monitoring plans so that they do not interfere with routine lab activity. References: 1. Regberg, A. B. et al. 49th Lunar and Planetary Science Conference (2018). 2. The United States Pharmacopeial Convention. USP General Chapter <1116> (2013). 3. Regberg, A. B., et al. 54th Lunar and Planetary Science Conference (2023). 4. Regberg, A. B. et al. 53rd Lunar and Planetary Science Conference ( 2022). 5. Davis, R. E.,et al. 50th Lunar and Planetary Science Conference (2019). 6. Qiagen. MagAttract® PowerMicrobiome® DNA/RNA EP Kit Handbook. (2018). 7. Walters, W. et al. mSystems 1, (2015). 8. Callahan, B. J. et al. Nat. Methods 13, 581–583 (2016). 9. Hall, M. & Beiko, R. G. Microbiome Analysis: Methods and Protocols113–129 (Springer, 2018). 10. Philipson, C. et al. Bio-Protoc. 7, e2622 (2017).

A. B. Regberg↗
DOE OSTI2026

Amapari Marker Band Metal‐Enrichments: Potential Mechanisms and Implications for Surface and Subsurface Water and Weathering in Gale Crater

NASA's Curiosity rover is exploring a 5 km tall sedimentary mound that is hypothesized to record the transition from a warm and wet (phyllosilicate-rich) to a cold and drier (sulfate-rich) Mars. Evidence of magnesium sulfate-bearing rock has shown that Curiosity has crossed through this phyllosilicate-sulfate transition. Recently, Curiosity arrived at the Amapari Marker Band, a darker, indurated unit that can be traced laterally for tens of kilometers in orbiter images. Here, Curiosity found evidence for a very broad lake, and bedforms interpreted as wave-ripple laminated sedimentary rock that likely was deposited in shallow water in the explored location, before becoming a deeper lake. These rocks are enriched in Fe, Mn, and Zn which has major implications for groundwater paleohydrology in Gale crater. Three formation hypotheses are considered: concretion formation during early diagenetic alteration of shallow lake sediments, laterization or leaching of the sediments, and addition of Fe, Mn, and Zn by a mildly acidic and reducing groundwater interacting with a redox and/or pH front in a stratified lake. The preferred interpretation of the metal enrichments within the Amapari Marker band sedimentary rocks is that they formed in a shallow water environment at a redox and/or pH front within the ripple unit, which drove precipitation and concentration of metals. If the enrichments are due to groundwater alteration, these processes could link subsurface and surface environments. Water and the presence of high amounts of redox sensitive elements and other metals are favorable indicators for habitability.

58 GEOSCIENCES↗