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At least 109 records · Page 6

Evolution of catalytic function

An RNA-based evolution system was constructed in the laboratory and used to develop RNA enzymes with novel catalytic function. By controlling the nature of the catalytic task that the molecules must perform in order to survive, it is possible to direct the evolving population toward the expression of some desired catalytic behavior. More recently, this system has been coupled to an in vitro translation procedure, raising the possibility of evolving protein enzymes in the laboratory to produce novel proteins with desired catalytic properties. The aim of this line of research is to reduce darwinian evolution, the fundamental process of biology, to a laboratory procedure that can be made to operate in the service of organic synthesis.

NASA Program Exobiology↗

Reverberation Chamber Uniformity Validation and Radiated Susceptibility Test Procedures for the NASA High Intensity Radiated Fields Laboratory

The NASA Langley Research Center's High Intensity Radiated Fields Laboratory has developed a capability based on the RTCA/DO-160F Section 20 guidelines for radiated electromagnetic susceptibility testing in reverberation chambers. Phase 1 of the test procedure utilizes mode-tuned stirrer techniques and E-field probe measurements to validate chamber uniformity, determines chamber loading effects, and defines a radiated susceptibility test process. The test procedure is segmented into numbered operations that are largely software controlled. This document is intended as a laboratory test reference and includes diagrams of test setups, equipment lists, as well as test results and analysis. Phase 2 of development is discussed.

Koppen, Sandra V.↗

Development of three-dimensional spatial displays using a geographically based information system

Procedures necessary for the development of a generalized three-dimensional perspective software capability in support of graphic, topographic, and color mapping of Landsat data are reviewed. The NASA Earth Resources Laboratory developed the procedures in order to facilitate the processing and analysis of disparate, geographically oriented base maps from aircraft and satellite sensors. Perspective displays are obtained through a translation of the space-viewed object to a vantage point coordinate system, followed by a rotation through two angles for alignment along the vantage line of sight, and finally a perspective transformation to yield two-dimensional displays with no hidden lines. Matrix equations for the transformations are reviewed, including scaling, and block diagrams are provided of the data and perspective software systems. The classification data plane may be mapped onto a topographic elevation data plane.

Junkin, B. G.↗

Chemical Hygiene Program

The Chemical Management Team is responsible for ensuring compliance with the OSHA Laboratory Standard. The program at Lewis Research Center (LeRC) evolved over many years to include training, developing Standard Operating Procedures (SOPS) for each laboratory process, coordinating with other safety and health organizations and teams at the Center, and issuing an SOP binder. The Chemical Hygiene Policy was first established for the Center. The Chemical Hygiene Plan was established and reviewed by technical, laboratory and management for viability and applicability to the Center. A risk assessment was conducted for each laboratory. The laboratories were prioritized by order of risk, higher risk taking priority. A Chemical Management Team staff member interviewed the lead researcher for each laboratory process to gather the information needed to develop the SOP for the process. A binder containing the Chemical Hygiene Plan, the SOP, a map of the laboratory identifying the personal protective equipment and best egress, and glove guides, as well as other guides for safety and health. Each laboratory process has been captured in the form of an SOP. The chemicals used in the procedure have been identified and the information is used to reduce the number of chemicals in the lab. The Chemical Hygiene Plan binder is used as a training tool for new employees. LeRC is in compliance with the OSHA Standard. The program was designed to comply with the OSHA standard. In the process, we have been able to assess the usage of chemicals in the laboratories, as well as reduce or relocate the chemicals being stored in the laboratory. Our researchers are trained on the hazards of the materials they work with and have a better understanding of the hazards of the process and what is needed to prevent any incident. From the SOP process, we have been able to reduce our chemical inventory, determine and implement better hygiene procedures and equipment in the laboratories, and provide specific training to our employees. As a result of this program, we are adding labeling to the laboratories for emergency responders and initiating a certified chemical user program.

Mayor, Antoinette C.↗

PPPL Engineering Review - Systems Subcommittee Report

Sandia and SLAC staff evaluated Princeton Plasma Physics Laboratory (PPPL) engineering procedures. The report documents, team members, interviewees and documents consulted, lines of inquiry, strengths and best practices, weaknesses and risks, opportunities for improvement, and general comments.

42 ENGINEERING↗

The Relationship Between Perceived Control and Hypothalamic–Pituitary–Adrenal Axis Reactivity to the Trier Social Stress Test in Healthy Young Adults

Psychological factors can modulate the hypothalamic–pituitary–adrenal (HPA) axis activity toward stressors. Animal studies demonstrated that uncontrollability was one critical factor associated with HPA axis stress response, but the results in human studies were inconsistent. The current study adopted a standardized laboratory stress induction procedure, the Trier Social Stress Test (the TSST), as the stressor to regulate the objective controllability level, and young adult participants were asked to rate their subjectively perceived control level toward the stressor and measured their cortisol stress responses ( N =54; 19 females and 35 males) to address this concern. Results showed that participants’ perceived control on the TSST was related to the cortisol stress response. In other words, under the stress of a certain objective controllability level, the lower the subjectively perceived control level, the greater the HPA axis response. This finding suggested that, in addition to objective controllability, subjectively perceived control is a psychological factor that regulates activation of the HPA axis in young adults.

Liu, Qian↗

Short communication: Mechanism and prevention of irreversible trapping of atmospheric He during mineral crushing

A pervasive challenge in noble gas geochemistry is to ensure that analytical techniques do not modify the composition of the noble gases in the samples. Noble gases are present in the atmosphere and are used in a number of manufacturing procedures and by laboratory equipment. Of particular concern is the introduction of atmospheric or laboratory noble gases to samples during preparation before samples are placed in a vacuum chamber for analysis. Recent work has shown the potential for contamination of crushed samples with air-derived He that is not released by placing the samples under vacuum at room temperature. Using pure He gas as a tracer, we show that the act of crushing samples to a fine powder itself can introduce He contamination but that this is easily avoided by crushing under liquid or in an inert atmosphere. Because the He is trapped during crushing, the same concern does not extend to samples that are naturally fine-grained when collected. We also show model results that demonstrate when this effect might significantly impact samples for (U–Th) / He geochronology or 3 He cosmogenic nuclide dating. The degree of He contamination from crushing samples to sizes smaller than the > 63 µm range typically used for geochronology is insignificant for samples with a date of at least 1 Ma and 1 ppm U, and the degree of He contamination from crushing samples to sizes smaller than the 100–500 µm range typically used for cosmogenic nuclide dating is also insignificant for samples with a date of at least 10 ka with typical 3 He production rates.

58 GEOSCIENCES↗

Standard operating procedures, water immersion facility, revision B

General guideline procedures to identify those factors that are common to all spacecraft design laboratory support group emergency procedures and to establish the basic rescue plan are presented. This eliminates needless repetition of the fundamentals from the other, more specific procedures.

Source record↗

A dc model for power switching transistors suitable for computer-aided design and analysis

A model for bipolar junction power switching transistors whose parameters can be readily obtained by the circuit design engineer, and which can be conveniently incorporated into standard computer-based circuit analysis programs is presented. This formulation results from measurements which may be made with standard laboratory equipment. Measurement procedures, as well as a comparison between actual and computed results, are presented.

Wilson, P. M.↗

Measurement of Aircraft Speed and Altitude

Problems involved in measuring speed and altitude with pressure-actuated instruments (altimeter, airspeed indicator, true-airspeed indicator, Machmeter, and vertical-speed indicator) are examined. Equations relating total pressure and static pressure to the five flight quantities are presented, and criteria for the design of total and static pressure tubes are given. Calibrations of typical static pressure installations (fuselage nose, wing tip, vertical fin, and fuselage vent) are presented, various methods for flight calibration of these installations are described, and the calibration of a particular installation by two of the methods is described in detail. Equations are given for estimating the effects of pressure lag and leaks. Test procedures for the laboratory calibration of the five instruments are described, and accuracies of mechanical and electrical instruments are presented. Operational use of the altimeter for terrain clearance and vertical separation of aircraft is discussed, along with flight technical errors and overall altitude errors of aircraft in cruise operations. Altitude-measuring techniques based on a variety of properties of the Earth and the atmosphere are included. Two appendixes present airspeed and altitude tables and sample calculations for determining the various flight parameters from measured total and static pressures.

Gracey, W.↗

Engine isolation for structural-borne interior noise reduction in a general aviation aircraft

Engine vibration isolation for structural-borne interior noise reduction is investigated. A laboratory based test procedure to simulate engine induced structure-borne noise transmission, the testing of a range of candidate isolators for relative performance data, and the development of an analytical model of the transmission phenomena for isolator design evaluation are addressed. The isolator relative performance test data show that the elastomeric isolators do not appear to operate as single degree of freedom systems with respect to noise isolation. Noise isolation beyond 150 Hz levels off and begins to decrease somewhat above 600 Hz. Coupled analytical and empirical models were used to study the structure-borne noise transmission phenomena. Correlation of predicted results with measured data show that (1) the modeling procedures are reasonably accurate for isolator design evaluation, (2) the frequency dependent properties of the isolators must be included in the model if reasonably accurate noise prediction beyond 150 Hz is desired. The experimental and analytical studies were carried out in the frequency range from 10 Hz to 1000 Hz.

Unruh, J. F.↗

Geophysical fluid dynamics

Systematic scaling or dimensional analysis reveals that certain scales of geophysical fluid flows (such as stellar, ocean, and planetary atmosphere circulations) can be accurately modeled in the laboratory using a procedure which differs from conventional engineering modeling. Rather than building a model to obtain numbers for a specific design problem, the relative effects of the significant forces are systematically varied in an attempt to deepen understanding of the effects of these forces. Topics covered include: (1) modeling a large-scale planetary atmospheric flow in a rotating cylindrical annulus; (2) achieving a radial dielectric body force; (3) spherical geophysical fluid dynamics experiments for Spacelab flights; (4) measuring flow and temperature; and (5) the possible effect of rotational or precessional disturbances on the flow in the rotating spherical containers.

Fowlis, W. W.↗

Design and test of aircraft engine isolators for reduced interior noise

Improved engine vibration isolation was proposed to be the most weight and cost efficient retrofit structure-borne noise control measure for single engine general aviation aircraft. A study was carried out the objectives: (1) to develop an engine isolator design specification for reduced interior noise transmission, (2) select/design candidate isolators to meet a 15 dB noise reduction design goal, and (3) carry out a proof of concept evaluation test. Analytical model of the engine, vibration isolators and engine mount structure were coupled to an empirical model of the fuselage for noise transmission evaluation. The model was used to develop engine isolator dynamic properties design specification for reduced noise transmission. Candidate isolators ere chosen from available product literature and retrofit to a test aircraft. A laboratory based test procedure was then developed to simulate engine induced noise transmission in the aircraft for a proof of concept evaluation test. Three candidate isolator configurations were evaluated for reduced structure-borne noise transmission relative to the original equipment isolators.

Unruh, J. F.↗

Investigation of accelerated stress factors and failure/degradation mechanisms in terrestrial solar cells

Results of an ongoing research program into the reliability of terrestrial solar cells are presented. Laboratory accelerated testing procedures are used to identify failure/degradation modes which are then related to basic physical, chemical, and metallurgical phenomena. In the most recent tests, ten different types of production cells, both with and without encapsulation, from eight different manufacturers were subjected to a variety of accelerated tests. Results indicated the presence of a number of hitherto undetected failure mechanisms, including Schottky barrier formation at back contacts and loss of adhesion of grid metallization. The mechanism of Schottky barrier formation is explained by hydrogen, formed by the dissociation of water molecules at the contact surface, diffusing to the metal semiconductor interface. This same mechanism accounts for the surprising increase in sensitivity to accelerated stress conditions that was observed in some cells when encapsulated.

Lathrop, J. W.↗

The use of dynamical constraints in the analysis of mesoscale rawinsonde data

A process for data interpolation which seeks to maintain dynamical consistency is applied to the analysis of mesoscale rawinsonde data obtained from the National Severe Storms Laboratory. The analysis procedure is in two steps: a successive-corrections interpolation to gridpoints, followed by a variational adjustment which uses a set of forecast equations of horizontal momentum as dynamical constraints. The objective analysis is applied in case studies of conditions on May 22, 1966 and June 8, 1966, and it is shown that the adjusted winds and geopotentials have greatly reduced residuals compared to the initial fields. Adjustments to the wind and geopotential fields have root mean square values of 1-2 m/sec and 30-50 sq m/sec per sec, respectively. In addition, the vertical velocity fields computed from the adjusted winds are found to be in closer agreement with actual weather data.

Bloom, S. C.↗

Science in space with the Space Station

The potential of the Space Station as a versatile scientific laboratory is discussed, reviewing plans under consideration by the NASA Task Force on Scientific Uses of the Space Station. The special advantages offered by the Station for expanding the scope of 'space science' beyond astrophysics, geophysics, and terrestrial remote sensing are stressed. Topics examined include the advantages of a manned presence, the scientific value and cost effectiveness of smaller, more quickly performable experiments, improved communications for ground control of Station experiments, the international nature of the Station, the need for more scientist astronauts for the Station crew, Station on-orbit maintenance and repair services for coorbiting platforms, and the need for Shuttle testing of proposed Station laboratory equipment and procedures.

Banks, Peter M.↗

Knowledge-based diagnosis for aerospace systems

The need for automated diagnosis in aerospace systems and the approach of using knowledge-based systems are examined. Research issues in knowledge-based diagnosis which are important for aerospace applications are treated along with a review of recent relevant research developments in Artificial Intelligence. The design and operation of some existing knowledge-based diagnosis systems are described. The systems described and compared include the LES expert system for liquid oxygen loading at NASA Kennedy Space Center, the FAITH diagnosis system developed at the Jet Propulsion Laboratory, the PES procedural expert system developed at SRI International, the CSRL approach developed at Ohio State University, the StarPlan system developed by Ford Aerospace, the IDM integrated diagnostic model, and the DRAPhys diagnostic system developed at NASA Langley Research Center.

Atkinson, David J.↗