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At least 109 records · Page 6

Planar Transformer With Asymmetric Integrated Leakage Inductance Using Horizontal Air Gap

This article presents a novel planar-based transformer winding and core structure with controllable leakage inductance generation for integrated magnetics applications. As a result of limitations in integrated magnetics from the literature, a new approach is proposed utilizing semi-interleaved windings and controllable leakage via a leakage flux core leg featuring a horizontal air gap. Here, the proposed transformer design is analyzed via detailed reluctance modeling to determine closed-form equations for the magnetizing and asymmetrically distributed leakage inductances. Next, a genetic-algorithm-based multiobjective design optimization problem is developed, seeking to minimize the core and winding losses of the proposed transformer subject to a set of parametric and geometric constraints in a dc-ac dual-active-bridge topology for microinverter applications. The optimization was extended to include other integrated magnetic structures from the literature, where it is determined that the proposed transformer is superior from the perspectives of efficiency, footprint area, and parasitic capacitance. Based on the results of the optimization analysis, two designs with theoretical transformer California Energy Commission (CEC) efficiency drops (i.e., CEC efficiency reduction specifically due to the transformer loss mechanisms) of 1.19% and 0.83% were fabricated and evaluated for electrical and thermal performance in the proposed 40 V, 400 W microinverter.

42 ENGINEERING↗

Diverse fates of ancient horizontal gene transfers in extremophilic red algae

Abstract Horizontal genetic transfer (HGT) is a common phenomenon in eukaryotic genomes. However, the mechanisms by which HGT‐derived genes persist and integrate into other pathways remain unclear. This topic is of significant interest because, over time, the stressors that initially favoured the fixation of HGT may diminish or disappear. Despite this, the foreign genes may continue to exist if they become part of a broader stress response or other pathways. The conventional model suggests that the acquisition of HGT equates to adaptation. However, this model may evolve into more complex interactions between gene products, a concept we refer to as the ‘Integrated HGT Model’ (IHM). To explore this concept further, we studied specialized HGT‐derived genes that encode heavy metal detoxification functions. The recruitment of these genes into other pathways could provide clear examples of IHM. In our study, we exposed two anciently diverged species of polyextremophilic red algae from the Galdieria genus to arsenic and mercury stress in laboratory cultures. We then analysed the transcriptome data using differential and coexpression analysis. Our findings revealed that mercury detoxification follows a ‘one gene‐one function’ model, resulting in an indivisible response. In contrast, the ars H gene in the arsenite response pathway demonstrated a complex pattern of duplication, divergence and potential neofunctionalization, consistent with the IHM. Our research sheds light on the fate and integration of ancient HGTs, providing a novel perspective on the ecology of extremophiles.

59 BASIC BIOLOGICAL SCIENCES↗

Domestication in dry-cured meat Penicillium fungi: Convergent specific phenotypes and horizontal gene transfers without strong genetic subdivision

Some fungi have been domesticated for food production, with genetic differentiation between populations from food and wild environments, and food populations often acquiring beneficial traits through horizontal gene transfers (HGTs). Studying their adaptation to human-made substrates is of fundamental and applied importance for understanding adaptation processes and for further strain improvement. We studied here the population structures and phenotypes of two distantly related Penicillium species used for dry-cured meat production, P. nalgiovense, the most common species in the dry-cured meat food industry, and P. salamii, used locally by farms. Both species displayed low genetic diversity, lacking differentiation between strains isolated from dry-cured meat and those from other environments. Nevertheless, the strains collected from dry-cured meat within each species displayed slower proteolysis and lipolysis than their wild conspecifics, and those of P. nalgiovense were whiter. Phenotypically, the non-dry-cured meat strains were more similar to their sister species than to their conspecific dry-cured meat strains, indicating an evolution of specific phenotypes in dry-cured meat strains. A comparison of available Penicillium genomes from various environments revealed HGTs, particularly between P. nalgiovense and P. salamii (representing almost 1.5 Mb of cumulative length). HGTs additionally involved P. biforme, also found in dry-cured meat products. We further detected positive selection based on amino acid changes. Our findings suggest that selection by humans has shaped the P. salamii and P. nalgiovense populations used for dry-cured meat production, which constitutes domestication. Several genetic and phenotypic changes were similar in P. salamii, P. nalgiovense and P. biforme, indicating convergent adaptation to the same human-made environment. Our findings have implications for fundamental knowledge on adaptation and for the food industry: the discovery of different phenotypes and of two mating types paves the way for strain improvement by conventional breeding, to elucidate the genomic bases of beneficial phenotypes and to generate diversity.

60 APPLIED LIFE SCIENCES↗

Wettability Effects on Falling Film Flow and Heat Transfer Over Horizontal Tubes in Jet Flow Mode

The performance of a falling-film heat exchanger is strongly linked to the surface characteristics and the heat transfer processes that take place over the tubes. The primary aim of this numerical study is to characterize the influence of surface wettability on the film flow behavior and its associated surface heat transfer in the jet-flow mode. Volume of fluid (VOF) based simulations are carried out for horizontal tubes with different surface wettabilities. The wettability of the tube surfaces is represented using the Kistler's dynamic contact angle model. Surface wettability effects ranging from superhydrophilic to superhydrophobic are studied by varying the equilibrium contact angle from 2 deg to 175 deg. Two different liquid mass flow rates of 0.06 and 0.18 kg/m-s corresponding to the inline and staggered jet flow modes are studied. Results are presented in terms of the liquid film thickness, the contact areas between the different phases (solid–liquid and liquid–air), and the heat transfer coefficient or Nusselt number. The resistance imposed by the increasing contact angles inhibits the extent of the liquid spreading over the tube surface, and this, in turn, influences the liquid film thickness, and the wetted area of the tube surface. A significant decrement in the heat transfer rate from the tube surfaces was observed as the equilibrium contact angle increased from 2 deg to 175 deg. The local distributions of the Nusselt number over the tube surface are strongly influenced by the flow recirculation in the liquid bulk.

Engineering↗

Estimating UV-B, UV-Erithemic, and UV-A Irradiances From Global Horizontal Irradiance and MERRA-2 Ozone Column Information

The ground ultraviolet (UV) solar radiation is relevant due to its impacts on plastics degradation (mainly UVA) and on human health (UVB and erithemic UV (UVE)). UV ground measurements are not as ubiquitous as the relatively common global horizontal irradiance (GHI) measurements. Three simple models that estimate the UVA, UVB, and UVE components of solar irradiance from GHI and ozone column information are locally adjusted and validated. Five one-minute datasets from three sites in southeastern South America and two in the United States are used for simultaneous solar irradiance and UV data. All sites correspond to temperate mid-latitude regions. Simultaneous atmospheric total ozone column information is obtained from the reanalysis modern-era retrospective analysis for research and applications (MERRA-2) database for each site. Aside from locally adjusted models, average models with a single set of coefficients are also evaluated. For instance, the best average model is able to estimate UVE with a typical uncertainty below 12% and mean biases between +-3%, relative to the average of the measurements. Similar results are reported for the UVB and UVA components. These results, which can be useful in regions with similar climate and geography, provide a simple way to estimate UV irradiance under all-sky conditions with known uncertainty. This is an alternative to global satellite-based UV estimates, which can have high uncertainties at specific locations. Because MERRA-2 information has a global coverage, when coupled with good satellite-based estimates for GHI, UV irradiances can be estimated by this method over a large territory.

environmental UV radiation↗

Horizontal Gene Transfer and CRISPR Targeting Drive Phage-Bacterial Host Interactions and Coevolution in “Pink Berry” Marine Microbial Aggregates

Phages, which are viruses that infect bacteria, are important components of all microbial systems, in which they drive the turnover of organic matter by lysing host cells, facilitate horizontal gene transfer (HGT), and coevolve with their bacterial hosts. Bacteria resist phage infection, which is often costly or lethal, through a diversity of mechanisms.

59 BASIC BIOLOGICAL SCIENCES↗

Assessing horizontal gene transfer in the rhizosphere of Brachypodium distachyon using fabricated ecosystems (EcoFABs)

ABSTRACT Horizontal gene transfer (HGT) is a major process by which genes are transferred between microbes in the rhizosphere. However, examining HGT remains challenging due to the complexity of mimicking conditions within the rhizosphere. Fabricated ecosystems (EcoFABs) have been used to investigate several complex processes in plant-associated environments. Here we show that EcoFABs are efficient tools to examine and measure HGT frequency in the rhizosphere. We provide the first demonstration of gene transfer via a triparental conjugation system in the Brachypodium distachyon rhizosphere in an EcoFAB using Pseudomonas putida KT2440 as both donor and recipient bacterial strain with the donor containing a mobilizable and non-self-transmissible plasmid. We observed that the frequency of plasmid transfer in the rhizosphere is potentially dependent on the plant developmental stage and the composition and amount of root exudates. The frequency of plasmid transfer also increased with higher numbers of donor cells. We demonstrate the transfer of plasmid from P. putida to another B. distachyon root colonizer, Burkholderia sp. OAS925, showing HGT within a rhizosphere microbial community. Environmental stresses also influenced the rate and efficiency of HGT in the rhizosphere between different species and genera. This study provides a robust workflow to evaluate transfer of engineered plasmids in the rhizosphere when such plasmids are potentially introduced in a field or other plant-associated environments. IMPORTANCE We report the use of EcoFABs to investigate the HGT process in a rhizosphere environment. It highlights the potential of EcoFABs in recapitulating the dynamic rhizosphere conditions as well as their versatility in studying plant-microbe interactions. This study also emphasizes the importance of studying the parameters impacting the HGT frequency. Several factors such as plant developmental stages, nutrient conditions, number of donor cells, and environmental stresses influence gene transfer within the rhizosphere microbial community. This study paves the way for future investigations into understanding the fate and movement of engineered plasmids in a field environment.

Priya, Shweta↗

Reconstruction of atmospheric neutrinos in DUNE’s horizontal-drift far-detector module

This paper reports on the capabilities in reconstructing and identifying atmospheric neutrino interactions in one of the Deep Underground Neutrino Experiment’s (DUNE) far detector modules, a liquid argon time projection chamber (LArTPC) with horizontal drift (FD-HD) of ionization electrons. The reconstruction is based upon the workflow developed for DUNE’s long-baseline oscillation analysis, with some necessary machine-learning models’ retraining and the addition of features relevant only to atmospheric neutrinos such as the neutrino direction reconstruction. Where relevant, the impact of the detection of the charged particles of the hadronic system is emphasized, and comparisons are carried out between the case when lepton-only information is considered in the reconstruction (as is the case for many neutrino oscillation experiments), versus when all particles identified in the LArTPC were included. Three neutrino direction reconstruction methods have been developed and studied for the atmospheric analyses: using lepton-only information, using all reconstructed particles, and using only correlations from reconstructed hits. The results indicate that incorporating more than just lepton information significantly improves the resolution of both neutrino direction and energy reconstruction. The angle reconstruction algorithms developed in this work result in no strong dependence on particle direction for reconstruction efficiencies or neutrino flavor identification. This comprehensive review of the reconstruction of atmospheric neutrinos in DUNE’s FD-HD LArTPC is the first step towards developing a first neutrino oscillation sensitivity analysis, which will ready DUNE for its first measurements.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

Effects of Horizontal Resolution, Domain Size, Boundary Conditions, and Surface Heterogeneity on Coarse LES of a Convective Boundary Layer

Atmospheric properties in a convective boundary layer vary over a wide range of spatial scales and are commonly studied using large-eddy simulations (LES) in various configurations. We examine how the boundary layer depth and distribution of variability across scales are affected by LES grid spacing, domain size, inhomogeneity of surface properties, and external forcing. Two different setups of the Weather Research and Forecasting (WRF) model are analyzed. A semi-idealized configuration uses a periodic domain, flat surface, prescribed homogeneous surface heat fluxes, and horizontally uniform profiles of large-scale advective tendencies. A nested LES setup employs a larger domain and realistic initial and boundary conditions, including an interactive land surface model with representative topography and vegetation and soil types. Subdomains of identical size are analyzed for all simulations. Characteristic structure sizes are quantified using the variability scales L 50 and L 95 , defined such that features smaller than that contain 50% and 95% of the total variance, respectively. Progressive increase in L 50 from vertical velocity to temperature and moisture structures is systematically reproduced in all simulation configurations. This dependence of L 50 on the considered variable complicates the development of scale-aware parameterizations for models with grid spacing in the “terra incognita”. In simulations using a larger domain with heterogeneous surface properties, the development of internal mesoscale patterns significantly affects variance distributions inside analyzed subdomains. Sizes of boundary layer structures also strongly depend on the LES grid spacing and, in case of heterogeneous surface and topography, on location of the subdomain inside a larger computational domain.

54 ENVIRONMENTAL SCIENCES↗

Effects of Atmosphere and Ocean Horizontal Model Resolution on Tropical Cyclone and Upper-Ocean Response Forecasts in Four Major Hurricanes

A coupled atmosphere–ocean model is necessary for tropical cyclone (TC) prediction to accurately characterize ocean feedback on atmospheric processes within the TC environment. Here, the ECMWF coupled global model is run at horizontal resolutions from 9 to 1.4 km in the atmosphere, as well as 25 and 8 km in the ocean, to identify how resolution impacts forecast accuracy of four observed major TCs in the Atlantic: Irma, Florence, Teddy, and Ida. Most of the resolutions used here are unprecedented for global models. GOES-16 and synthetic aperture radar (SAR) satellite images and best track data are used for atmospheric validation. Salinity and temperature observations from Air-Launched Autonomous Micro-Observer (ALAMO) floats are used to validate modeled upper-ocean response, including mixed layer deepening, sea surface cooling, and near-inertial waves in the wakes of TCs. Increasing atmospheric resolution leads to more realistic TC structure and stronger winds, significantly improving TC intensity forecasts and modestly improving track errors. Ocean resolution impacts the upper-ocean response but does not influence atmospheric forecasts for the fast-moving TCs considered here. Stronger mixing, sea surface cooling, and near-inertial oscillations are found for both higher atmosphere and ocean resolutions, provided the initial upper-ocean state is the same for the two ocean resolutions. Whether this agrees better with the ALAMO observations also depends on the realism of the initial upper-ocean state in the model, emphasizing the importance of ocean initialization for the accurate upper-ocean response. Overall, the model at all resolutions correctly predicts stronger mixing, surface cooling, and near-inertial oscillation amplitudes to the right of a TC center, as observed by ALAMO floats.

Atmosphere-ocean interaction↗

Granick revisited: Synthesizing evolutionary and ecological evidence for the late origin of bacteriochlorophyll via ghost lineages and horizontal gene transfer

Photosynthesis—both oxygenic and more ancient anoxygenic forms—has fueled the bulk of primary productivity on Earth since it first evolved more than 3.4 billion years ago. However, the early evolutionary history of photosynthesis has been challenging to interpret due to the sparse, scattered distribution of metabolic pathways associated with photosynthesis, long timescales of evolution, and poor sampling of the true environmental diversity of photosynthetic bacteria. Here, we reconsider longstanding hypotheses for the evolutionary history of phototrophy by leveraging recent advances in metagenomic sequencing and phylogenetics to analyze relationships among phototrophic organisms and components of their photosynthesis pathways, including reaction centers and individual proteins and complexes involved in the multi-step synthesis of (bacterio)-chlorophyll pigments. We demonstrate that components of the photosynthetic apparatus have undergone extensive, independent histories of horizontal gene transfer. This suggests an evolutionary mode by which modular components of phototrophy are exchanged between diverse taxa in a piecemeal process that has led to biochemical innovation. We hypothesize that the evolution of extant anoxygenic photosynthetic bacteria has been spurred by ecological competition and restricted niches following the evolution of oxygenic Cyanobacteria and the accumulation of O 2 in the atmosphere, leading to the relatively late evolution of bacteriochlorophyll pigments and the radiation of diverse crown group anoxygenic phototrophs. This hypothesis expands on the classic “Granick hypothesis” for the stepwise evolution of biochemical pathways, synthesizing recent expansion in our understanding of the diversity of phototrophic organisms as well as their evolving ecological context through Earth history.

58 GEOSCIENCES↗

Horizontal Splitter Design For FFA@CEBAF Energy Upgrade: Current Status

Thomas Jefferson National Accelerator Facility (Jefferson Lab) is currently studying the feasibility of an energy upgrade based upon Fixed-Field Alternating Gradient (FFA) permanent magnet technology. The current plan is to replace the highest-energy recirculation arcs with FFA arcs, increasing the total number of beam recirculations, thus the energy. In order to accommodate multiple passes in the FFA arcs, horizontal splitters are being designed to control the beam parameters entering the FFA arcs, as well as the time of flight and R_56. In the current design, six passes will recirculate through the FFA arcs, necessitating the design of six independent beamlines to control the optics and beam dynamics matching into the arcs. These beamlines must fit into the current CEBAF tunnel, while allowing for personnel and equipment access. They must also be flexible enough to accommodate the beam under realistic operational conditions and fluctuations. The constraints on the system are highly restrictive, complicating the design. This document will describe the current state of the design, and indicate the work remaining for a complete conceptual design.

Benesch, Jay↗

First results of AUP Nb3Sn quadrupole horizontal tests

The Large Hadron Collider will soon undergo an upgrade to increase its luminosity by a factor of ~10 [1]. A crucial part of this upgrade will be replacement of the NbTi focusing magnets with Nb3Sn magnets that achieve a ~50% increase in the field strength. This will be the first ever large-scale implementation of Nb3Sn magnets in a particle accelerator. The High-Luminosity LHC Upgrade, HL-LHC is a CERN project with a world-wide collaboration. It is under construction and utilizes Nb3Sn Magnets (named MQXF) as key ingredients to increase tenfold the integrated luminosity delivered to the CMS and ATLAS experiments in the next decade. The HL-LHC AUP is the US effort to contribute approximately 50% of the low-beta focusing magnets and crab cavities for the HL-LHC. This paper will present the program to fabricate the Nb3Sn superconducting magnets. We are reporting the status of the HL-LHC AUP project present the results from horizontal tests of the first fully assembled cryo-assembly.

43 PARTICLE ACCELERATORS↗

Current status of conceptual horizontal splitter design for FFA@CEBAF energy Upgrade

Jefferson Lab’s Continuous Electron Beam Accelerator Facility (CEBAF) is currently investigating the feasibility of upgrading its maximum operating energy using Fixed-Field Alternating-gradient (FFA) recirculating arcs to increase the total number of recirculations of the beam through the pair of LINACs. These FFA arcs will be composed of permanent magnets, with small Panofsky-style multipole correctors. In order to control the beam parameters through these FFA arcs, horizontal splitters must be used. The geometrical and physical constraints, as well as the beam matching requirements are very restrictive, complicating the design. This work will show the current status of the most mature design, which includes matching solutions, as well as options for extraction of the beam.

Accelerator Physics↗

Status of conceptual horizontal splitter design for FFA@CEBAF energy upgrade

Jefferson Lab’s Continuous Electron Beam Accelerator Facility (CEBAF) is currently investigating the feasibility of upgrading its maximum operating energy using Fixed-Field Alternating-gradient (FFA) recirculating arcs to increase the total number of recirculations the beam through the pair of LINACs. These FFA arcs will be composed of permanent magnets, with small Panofsky-style multipole correctors. Horizontal splitters are needed to control the beam parameters through these FFA arcs. The geometrical and physical constraints, as well as the beam matching requirements are very restrictive, complicating the design. This work will show the current status of the most mature design, which includes matching solutions, as well as options for extraction of the beam.

Accelerator Physics↗

Impact of Polymer Rheology on Gel Treatment Performance of Horizontal Wells with Severe Channeling

Summary Gel treatment has been a cost-effective method to control the conformance of a reservoir with severe heterogeneity problems. The water channels in such reservoirs can be classified as open fractures or high permeability porous media with pore-throat network. Many simulation studies have been conducted to discuss gel treatment performance for conformance control. However, nobody considered the polymer rheology difference in open fractures and porous media in simulation. Previous simulation studies also ignored the residual resistance factor as a function of rock permeability rather than a constant parameter. In this study, a conceptual simulation model was established to simulate the linear flow system for the reservoir with horizontal wells considering the two factors mentioned above. The results demonstrate that the gel treatment always provides the better placement results in the open fracture type channel than pore-throat network type channel. Moreover, it is very necessary to consider residual resistance factor as a function of permeability, which is based on the experimental results and can provide much greater plugging efficiency in the higher permeable channels than constant residual resistance factor. Sensitivity analysis studies and multifactor analysis indicate that increasing oil viscosity and permeability ratio has a strong positive influence on conformance control results, which indicate in-situ gel treatment can be better applied in heavy or viscous oil reservoirs with fracture-like channels. Besides, the results also indicate that in reservoirs with severe channeling problem where channel velocity was high, the differences of gelant placement and profile improvement in models with two different types of channels could be enlarged greatly.

Engineering↗

IER-539 CED-1: Preliminary Design of a New Horizontal Split Table

This report presents the preliminary design (CED-1) of IER-539, focused on a new Horizontal Split Table (HST) critical assembly machine. The Department of Energy (DOE) National Nuclear Criticality Safety Program (NCSP) funds critical experiment R&D and its Mission and Vision document identifies a long-standing technical gap of a general purpose HST. HSTs have historically been used for experiments that are impractical to assemble on a vertical lift machine (VLM), usually due to their large physical size or weight. While many HSTs were historically employed in the US (including at LLNL), there are currently no surviving HSTs in the US. While there are two critical experiments facilities remaining in the US, the National Critical Experiments Research Center (NCERC) operated by LANL and the Critical Experiments Facility (CX) operated by SNL, these facilities are not equipped with a general purpose HST. There are a number of experiment designs that require an HST, including needs for Defense Programs and Nuclear Energy. Lack of an HST constraints the national ability to perform large and heavy experiments. Large footprints are needed to successfully create mockup experiments for advanced reactors (mock-up cold critical reactor cores, which is a urgent need for new reactor designs using novel materials and longer cycle lengths), to create solution experiments in lattices, or to test nuclear data of heavy metals. A new HST is a vital piece of criticality experiment infrastructure that would allow for the conduct of integral critical experiments to address needs in the nuclear criticality safety, nuclear data, and other DOE stakeholder communities.

42 ENGINEERING↗

Review and Analysis of Heat Transfer Correlations For Horizontal Pseudocritical CO 2 Heat Exchanger Applications

There is significant interest in the development of supercritical carbon dioxide (sCO 2 ) power cycles because of the potential for smaller and more energy efficient systems than a steam Rankine cycle. Heat exchanger designs typically use empirical correlations, but the applicability of these correlations near the CO 2 critical point is a potential issue. Though numerous correlations have been proposed in the literature, there are some disagreements when it comes to the accuracy. The current work recognizes the role of thermophysical properties, and its impacts on the heat transfer correlations and cycle efficiency. Heat transfer correlations proposed for horizontal flow inside circular pipes were analyzed with the help of numerical simulations. Steady state RANS simulations were performed using SST k-w turbulence model to evaluate the Nusselt number empirical correlations. It was found that the most of correlations (except Yoon) produced a Nusselt number that differed significantly with the one predicted numerically. Some of the correlations were developed for pure forced convection regime and as mentioned in Lin et al. do not account for mixed convection or free convection effects. Based on the limited observation, it appears that Yoon et al. predictions match well with the numerically predicted Nusselt Numbers. However, further analysis is required understand the applicability of various correlations and is contingent on accurate measurements or predictions of wall temperature profiles in the axial and the circumferential directions.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗