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At least 109 records · Page 6

Mauna Loa Aerosol Backscatter Intecomparison Experiment (MABIE)

Measurements of CO2 aerosol backscatter coefficients and other aerosol physicochemical properties were obtained in remote Pacific free tropospheric airmasses at the Mauna Loa Observatory in Hawaii. The experiment used an attenuated total reflection impactor (Johnson et al., 1983), a dispersive infrared spectrophotometer, and a high-resolution optical particle counter. Preliminary results suggest that sulfate compounds dominate background backscatter properties at CO2 wavelengths between 9 and 10 microns.

Bowdle, David A.↗

The high-resolution spectrum of the pulsating, pre-white dwarf star PG 1159-035 (GW VIR)

High-resolution and low-resolution UV spectra and a high-resolution optical spectrum were obtained for PG 1159-035, revealing apparent photospheric absorption features with defined cores from N V 1240 A, N IV 1270 A, O V 1371 A, and C IV 1550 A. The photospheric velocity derived using all of these lines except for C IV is about +35 km/s. Equivalent-width measurements determined for all of the features may provide a tighter constraint on the photospheric temperature in a detailed model atmosphere analysis treating the CNO ions.

Liebert, James↗

Nucleation and growth control in protein crystallization

The five topics summarized in this final report are as follows: (1) a technique for the expedient, semi-automated determination of protein solubilities as a function of temperature and application of this technique to proteins other than lysozyme; (2) a small solution cell with adjustable temperature gradients for the growth of proteins at a predetermined location through temperature programming; (3) a microscopy system with image storage and processing capability for high resolution optical studies of temperature controlled protein growth and etching kinetics; (4) growth experiments with lysozyme in thermosyphon flow ; and (5) a mathematical model for the evolution of evaporation/diffusion induced concentration gradients in the hanging drop protein crystallization technique.

Rosenberger, Franz↗

High resolution lunar radar studies: Preliminary results

High resolution radar data for the lunar surface were acquired over 14 sites in June and November 1990 using the new 10 MHz data taking system at the Arecibo Observatory. The raw data collected for each site covers an area approximately 100 by 400 km and will be processed using delay-Doppler techniques into images of backscatter cross section with three fo four independent looks. All observations transmitted a circularly polarized signal and both senses of circular polarization were received containing the polarized and depolarized component of the backscatter signal. The relative power in these two polarizations provides useful information on properties of the surface, in particular surface roughness. The effort to date focused on the initial data analysis with new software written to perform a full synthetic aperture focusing on the raw radar data. This analysis will involve the use of complementary high resolution optical and topographic data sets to aid interpretation of surface scattering mechanisms.

Stacy, Nicholas J. S.↗

Hydrogen bonding in the benzene-ammonia dimer

High-resolution optical and microwave spectra of the gas-phase benzene-ammonia dimer were obtained, showing that the ammonia molecule resides above the benzene plane and undergoes free, or nearly free, internal rotation. To estimate the binding energy (De) and other global properties of the intermolecular potential, theoretical calculations were performed for the benzene-ammonia dimer, using the Gaussian 92 (Fritsch, 1992) program at the MP2/6-31G** level. The predicted De was found to be at the lowest end of the range commonly accepted for hydrogen bonding and considerably below that of C6H6-H2O, consistent with the gas-phase acidities of ammonia and water. The observed geometry greatly resembles the amino-aromatic interaction found naturally in proteins.

Rodham, David A.↗

Convective flow effects on protein crystal growth

The experimental setup for the in-situ high resolution optical monitoring of protein crystal growth/dissolution morphologies was substantially improved. By augmenting the observation system with a temperature-controlled enclosure, laser illumination for the interferometric microscope, and software for pixel by pixel light intensity recording, a height resolution of about two unit cells for lysozyme can now be obtained. The repartitioning of Na(+) and Cl(-) ions between lysozyme solutions and crystals was studied. Quite unexpectedly, it was found that the longer crystals were in contact with their solution, the lower was their ion content. The development of a model for diffusive-convective transport and resulting distribution of the growth rate on facets was completed. Results obtained for a realistic growth cell geometry show interesting differences between 'growth runs' at 1g and 0g. The kinematic viscosity of lysozyme solutions of various supersaturations and salt concentrations was monitored over time. In contrast to the preliminary finding of other authors, no changes in viscosity were found over four days. The experimental setup for light scattering investigations of aggregation and nucleation in protein solutions was completed, and a computer program for the evaluation of multi-angle light scattering data was acquired.

Rosenberger, Franz↗

Metal-line absorption at Z(sub abs) approximately Z(sub em) from associated galaxies

For a preliminary study of whether C IV absorption at Z(sub abs) approximately Z(sub em) is related to associated galaxy companions, we have collected data from a sample of 10 quasars with 0.15 less than z less than 0.65 for which high-resolution optical and UV spectroscopy is available from the literature, and for which we have deep optical images and limited spectroscopy. We also present new optical spectra for two of our samples. Four of these quasars have associated C IV absorption systems. In thes four fields, there are eight galaxies with M(sub r) less than -19.0 mag within 35 kpc of the quasar (projected distance, assuming they are at the quasar redshift), which may be candidates for the associated C IV absorption. This observed density of galaxies near quasars with associated C IV absorption is significantly greater than that for a control sample of quasars chosen from the literature. This result suggests that galaxies near the quasar line of sight may be linked with associated C IV absorption. None of these quasars show associated Mg II absorption, despite the presence of galaxies very near the line of sight, suggesting a Mg II 'proximity effect,' where ionizing flux from the quasar destroys the Mg(+) from at least the outer parts of the galaxies. Three quasars are located in rich galaxy clusters, but none of these quasars are found to have associated C IV absorption. This suggests that galaxies in rich clusters associated with quasars are less likely to be metal-line absorbers. It is plausible that the extended galaxy halos which may be responsible for the absorptions are stripped from galaxies in these dense environments. While it seems that at Z approximately 0.6 rich clusters do not cause them, associated C IV absorption systems at higher redshift may be explained by associated clusters if there has been evolution in the properties of galaxy halos in dense environments.

Ellingson, E.↗

An Experimental Investigation on the Static Equilibria and Dynamics of Liquid Bridges

A liquid bridge is a volume of liquid held between two or more solid supports. In the case of small disk supports with a sharp edge, the contact line between the bridge and the support disk will be anchored along the edge of the disk. For these cases the solid presents a geometrical singularity and the contact angle is indeterminate within a given range. This dissertation presents research conducted on liquid bridges with anchored contact lines. The three major topics covered are: determining the role of support geometry on static equilibria, liquid bridge dynamical behavior, and forces exerted by a liquid bridge on a support structure. The work was primarily experimental and conducted in a "Plateau tank" that allowed for the simulation of equivalent low-gravity conditions. The main thrust of the experimental work involved the use of a high resolution optical measurement system for imaging the dynamic zone shape, measurement of the static and dynamic contact angles and non-invasive analysis of excited surface modes. The liquid bridge was manipulated by computer controlled linear actuators which allowed precise control over the physical characteristics of the bridge. Experiments have been carried out to locate a bifurcation point along the maximum volume axisymmetric stability margin. Below the critical slenderness the bifurcation from an axisymmetric to a stable nonaxisymmetric configuration is supercritical. However, above this critical slenderness, the bifurcation is subcritical. A series of experiments analyzed the effect on axisymmetric bridge stability by using support disks of different radii, The shape behavior as transition points were approached, as well as the limiting case of a vanishing support radius was investigated. Experiments were performed to determine the resonant frequencies of axisymmetric bridges subject to lateral vibrations. Anomolous results led to a series of experiments to characterize nonlinearities present in the dynamic bridge shape. Finally, an attempt was made to experimentally measure the force exerted by the bridge on the lower support disk. This was done through use of a force balance apparatus. Particular attention was paid to the behavior of the bridge as the minimum volume stability limit was approached.

Resnick, Andrew Howard↗

NGST: Exploring the Fossil Record of Galaxy Formation

During this grant period the investigator has accomplished the following: developed and researched the science case for high resolution optical imaging with NGST (Next Generation Space Telescope); presented to the ASWG (Ad-Hoc Science Working Group) on the use of NGST to image and measure the proper motions of white dwarf stars that could account for the MACHO events toward the LMC (Large Magellanic Cloud); wrote proposals for the Design Reference Mission. Three proposals have been written on the following topics: Measuring the faint end of the white dwarf luminosity function to get an independent measure of the age of the oldest stars, measurement of the stellar luminosity function over the full range of age, abundance, and population type, and deep imaging of Local Group halos to measure the relative ages of the oldest stars in the Milky Way and other galaxies; he has introduced simulations of crowded field stellar photometry on a hypothetical population in the M31 halo. Using a physically correct luminosity function and a surface brightness of 24.5 mag/sq. arcsec, NGST imaging in 10 hours easily measures the turnoff and reaches 3 mags down the main sequence in the halo of M31; and has delivered talks at the NGST workshop in Liege, and at the meeting in Paris entitled "Connecting the Distant Universe with the Local Fossil Record".

Rich, R. Michael↗

Intrinsic Kinetics Fluctuations as Cause of Growth Inhomogeneity in Protein Crystals

Intrinsic kinetics instabilities in the form of growth step bunching during the crystallization of the protein lysozyme from solution were characterized by in situ high-resolution optical interferometry. Compositional variations (striations) in the crystal, which potentially decrease its utility, e.g., for molecular structure studies by diffraction methods, were visualized by polarized light reflection microscopy. A spatiotemporal correlation was established between the sequence of moving step bunches and the striations.

Vekilov, Peter G.↗

Effects of Convective Solute and Impurity Transport in Protein Crystal Growth

High-resolution optical interferometry was used to investigate the effects of forced solution convection on the crystal growth kinetics of the model protein lysozyme. Most experiments were conducted with 99.99% pure protein solutions. To study impurity effects, approx. 1% of lysozyme dimer (covalently bound) was added in some cases. We show that the unsteady kinetics, corresponding to bunching of growth steps, can be characterized by the Fourier components of time traces of the growth rate. Specific Fourier spectra are uniquely determined by the solution conditions (composition, temperature, and flow rate) and the growth layer source activity. We found that the average step velocity and growth rate increase by approx. I0% with increasing flow rate, as a result of the enhanced solute supply to the interface. More importantly, faster convective transport results in lower fluctuation amplitudes. This observation supports our rationale for system-dependent effects of transport on the structural perfection of protein crystals. We also found that solution flow rates greater than 500 microns/s result in stronger fluctuations while the average growth rate is decreased. This can lead to growth cessation at low supersaturations. With the intentionally contaminated solutions, these undesirable phenomena occurred at about half the flow rates required in pure solutions. Thus, we conclude that they are due to enhanced convective supply of impurities that are incorporated into the crystal during growth. Furthermore, we found that the impurity effects are reduced at higher crystal growth rates. Since the exposure time of terraces is inversely proportional to the growth rate, this observation suggests that the increased kinetics instability results from impurity adsorption on the interface. Finally, we provide evidence relating earlier observations of "slow protein crystal growth kinetics" to step bunch formation in response to nonsteady step generation.

Vekilov, Peter G.↗

The Role of Bearing and Scan Mechanism Life Testing in Flight Qualification of the MODIS Instrument

The Moderate Resolution Imaging Spectroradiometer (MODIS) is an instrument aboard the Terra (EOS AM-11) satellite and has been operating successfully since December 1999. MODIS has been viewing the entire Earth's surface and gathering data to better understand the global dynamics and processes occurring on land, in the oceans, and in the lower atmosphere. All observations are made through an extremely high resolution, optically and mechanically precise, scan mirror motor/encoder assembly. The reliable performance of this assembly depends on two duplex bearing pairs lubricated with Pennzane, a synthetic hydrocarbon, formulated with lead napthanate. This paper describes the results of accelerated and operational life tests. It also describes the post-test analyses of the disassembled bearings. Analyses were performed using micro-Raman, micro-FTIR, X-ray Photoelectron Spectroscopy (XPS), Scanning Electron Microscopy (SEM), and Size Exclusion Chromatography (SEC). In general, the three sets of bearings in each of the test stations were in very good condition after accumulating 68, 144, and 209 million revolutions, respectively. Some of the bearings exhibited lubricant degradation, indicated by viscous lubricant deposits on the cage and raceways.

VanDyk, Steven G.↗

The Role of Bearing and Scan Mechanism Life Testing in Flight Qualification of the MODIS Instrument

The Moderate Resolution Imaging Spectroradiometer (MODIS) is an instrument aboard the Terra (EOS AM-1) satellite and has been operating successfully since December 1999. MODIS has been viewing the entire Earth's surface and gathering data to better understand the global dynamics and processes occurring on land, in the oceans, and in the lower atmosphere. All observations are made through an extremely high resolution, optically and mechanically precise, scan mirror motor/encoder assembly. The reliable performance of this assembly depends on two duplex bearing pairs lubricated with Pennzane, a synthetic hydrocarbon, formulated with lead napthanate. This paper describes the results of accelerated and operational life tests. It also describes the post-test analyses of the disassembled bearings. Analyses were performed using micro-Raman, micro-FTIR, X-ray Photoelectron Spectroscopy (XPS), Scanning Electron Microscopy (SEM), and Size Exclusion Chromatography (SEC). In general, the three sets of bearings in each of the test stations were in very good condition after accumulating 68, 144, and 209 million revolutions, respectively. Some of the bearings exhibited lubricant degradation, indicated by viscous lubricant deposits on the cage and raceways.

VanDyk, Steven G.↗

Abundances in the Planetary Nebula IC 5217

High resolution optical wavelength spectroscopic data were secured in the optical wavelengths, 3700A - 10,050A, for the planetary nebula IC 5217 with the Hamilton Echelle Spectrograph at Lick Observatory. These optical spectra have been analyzed along with the near-UV and UV archive data. Diagnostic analyses indicate a nebular physical condition with electron temperature of about 10,700 K (from the [O III] lines) and the density of N(sub epsilon) = 5000/cm. Ionic concentrations have been derived with the representative diagnostics, and with the aid of a photoionization model construction, we derived the elemental abundances. Contrary to the previous studies found in the literature, He and C appear to be depleted compared to the average planetary nebula and to the Sun (and S marginally so), while the remaining elements appear to be close to the average value. IC 5217 may have evolved from an O-rich progenitor and the central star temperature of IC 5217 is likely to be 92,000 K.

Hyung, Siek↗

A simple fiber-optic microprobe for high resolution light measurements: application in marine sediment

A fiber-optic microphobe is described which is inexpensive and simple to build and use. It consists of an 80-micrometers optical fiber which at the end is tapered down to a rounded sensing tip of 20-30-micrometers diameter. The detector is a hybrid photodiode/amplifier. The probe has a sensitivity of 0.01 microEinst m-2 s-1 and a spectral range of 300-1,100 nm. Spectral light gradients were measured in fine-grained San Francisco Bay sediment that had an undisturbed diatom coating on the surface. The photic zone of the mud was only 0.4 mm deep. Measured in situ spectra showed extinction maxima at 430-520, 620-630, 670, and 825-850 nm due to absorption by chlorophyll a, carotenoids, phycocyanin, and bacterio-chlorophyll a. Maximum light penetration in the visible range was found in both the violet and the red < or = 400 and > or = 700 nm.

NASA Center ARC↗

Steps in Solution Growth: Revised Gibbs-Thomson Law, Turbulence and Morphological Stability

Two groups of new phenomena revealed by AFM and high resolution optical interferometry on crystal faces growing from solutions will be discussed. 1. Spacing between strongly polygonized spiral steps with low less than 10(exp -2) kink density on lysozyme and K- biphtalate do not follow the Burton-cabrera-Frank theory. The critical length of the yet immobile first Short step segment adjacent to a pinning defect (dislocation, stacking fault) is many times longer than that following from the step free energy. The low-kink density steps are typical of many growth conditions and materials, including low temperature gas phase epitaxy and MBE. 2. The step bunching pattern on the approx. 1 cm long { 110) KDP face growing from the turbulent solution flow (Re (triple bonds) 10(exp 4), solution flow rate approx. 1 m/s) suggests that the step bunch height does not increase infinitely as the bunch path on the crystal face rises, as is usually observed on large KDP crystals. The mechanism controlling the maximal bunch width and height is based on the drag of the solution depleted by the step bunch down thc solution stream. It includes splitting, coagulation and interlacing of bunches

Chernov, A. A.↗

The mass and speed dependence of meteor air plasma temperatures

The speed and mass dependence of meteor air plasma temperatures is perhaps the most important data needed to understand how small meteoroids chemically change the ambient atmosphere in their path and enrich the ablated meteoric organic matter with oxygen. Such chemistry can play an important role in creating prebiotic compounds. The excitation conditions in various air plasma emissions were measured from high-resolution optical spectra of Leonid storm meteors during NASA's Leonid Multi-Instrument Aircraft Campaign. This was the first time a sufficient number and range of temperature measurements were obtained to search for meteoroid mass and speed dependencies. We found slight increases in temperature with decreasing altitude, but otherwise nearly constant values for meteoroids with speeds between 35 and 72 km/s and masses between 10(-5) g and 1 g. We conclude that faster and more massive meteoroids produce a larger emission volume, but not a higher air plasma temperature. We speculate that the meteoric plasma may be in multiphase equilibrium with the ambient atmosphere, which could mean lower plasma temperatures in a CO(2)-rich early Earth atmosphere.

Temperature↗

Tomography of a Gamma-ray Burst Progenitor and its Host Galaxy

We have obtained near-infrared and high-resolution optical spectroscopy of the bright afterglow of the very intense gamma-ray burst recorded on 2002, October 4 (GRB 021004). Besides of line emission in the near-IR allowing an independent measurement of the systemic redshift (z = 2.3304 plus or minus 0.0005), we find several absorption line groups spanning a range of about 3,000 kilometers per second in velocity relative to the redshift of the host galaxy. The absorption profiles are very complex with both velocity-broadened components extending over several 100 kilometers per second and narrow lines with velocity widths of only approximately 20 kilometers per second. By analogy with QSO absorption line studies, the relative velocities, widths, and degrees of ionization of the lines ("line-locking", "ionization-velocity correlation") show that the progenitor had both an extremely strong radiation field and several distinct mass loss phases (winds). These results are consistent with GRB progenitors being massive stars, such as Luminous Blue Variables (LBVs) or Wolf-Rayet stars, providing a detailed picture of the spatial and velocity structure of the GRB progenitor star at the time of explosion. The host galaxy is a prolific star-forming galaxy with a SFR of approximately 10 solar mass yr(sup -l).

Castro-Tirado, Alberto J.↗