Effects of cadmium and copper treatments and water stress on the thermal emission from peas /Pisum sativum L./ - Controlled environment experiments
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Emphasis was given to environmental factors that alter the flux of carbohydrate from the shoot to the root system to support the absorption of nitrogen and the subsequent interaction between nitrogen uptake and whole plant growth. Only the nitrate form of nitrogen was utilized in order to establish base line responses for evaluation of future studies utilizing both ammonium and nitrate.
A research project in the food production group of the Closed Ecological Life Support System (CELSS) program sought to define optimum conditions for photosynthetic productivity of a higher plant food crop. The effects of radiation and various atmospheric compositions were studied.
Utilizing high-frequency data from a highly instrumented rotor assembly, seeded bearing defect signatures are characterized using both conventional linear approaches, such as power spectral density analysis, and recently developed nonlinear techniques such as bicoherence analysis. Traditional low-frequency (less than 20 kHz) analysis and high-frequency envelope analysis of both accelerometer and acoustic emission data are used to recover characteristic bearing distress information buried deeply in acquired data. The successful coupling of newly developed nonlinear signal analysis with recovered wideband envelope data from accelerometers and acoustic emission sensors is the innovative focus of this research.
The widespread application of electric (often called artificial) light in greenhouses, growing rooms, and plant growth chambers would presuppose that the role of phytochrome would be considered in the selection and use of such lighting systems. Unfortunately this is not usually the case. Part of the problem is that many students, and indeed an unfortunate number of senior scientists, seem to regard phytochrome as a laboratory phenomenon without much application in the real world. They simply have not grasped the concept that phytochrome is functioning through all stages of plant development, wherever plants are grown. It is certainly true, as Meijer (1971) stated, that one cannot compare experimental results obtained under very strict laboratory conditions with plant irradiation in glasshouses and in growth rooms. When Karl Norris developed the first practical portable spectroradiometer about 1962, some of the first measurements were to determine the red/far-red ratios under tree canopies. These measurements showed clearly the predominance of far-red in the understory and suggested that far-red was contributing to the elongation exhibited by many species growing in the shade, and possibly was a factor in the induction of light requirements in seeds. Subsequently we used Catalpa leaves as far-red filters to make light-insensitive lettuce seed light requiring. Much more detailed work has since been done on phytochrome effects in the natural environment, and it is encouraging to note that efforts are bring made to apply phytochrome research to horticulture.
These guidelines for lighting of plants, established by the organizing committee, provided a focus for the discussions at the workshop. The guidelines served as a basis for discussion amongst the workshop attendees and led to a number of recommendations that were recorded by the session chairpersons. The organizing committee indicated they would incorporate the recommendations and suggestions into a revised set of guidelines for additional discussion. It was understood that these guidelines will not be standards and will require upgrading and modifications as lamps and equipment become available and as new insights are obtained on plants' response to light. Revised draft guidelines are included as Tables 1 and 2 that have been developed by the organizing committee following the suggestions obtained at the workshop. The guidelines for growth chambers are in Table 1 and guideines for greenhouses are given in Table 2. These have been distributed to the participants who indicated a willingness to review proposals that were developed. It is hoped that these proposals will lead to the development of guidelines that will have general acceptance by plant scientists.
Volatile organic compound(VOC), ethylene gas, was characterized and quantified by GC/FID. 20-50 ppb levels were detected during the growth stages of radish. SPME could be a good analytical tool for the purpose. Low temperature trapping method using dry ice/diethyl ether and liquid nitrogen bath was recommended for the sampling process for GC/PID and GC/MS analysis.
Sweetpotato is one of several crops recommended by National Aeronautics and Space Administration (NASA) for bioregenerative life support studies. One of the objectives of the Tuskegee University NASA Center is to optimize growth conditions for adaptability of sweetpotatoes for closed bioregenerative systems. The role of nutrient solution management as it impacts yield has been one of the major thrusts in these studies. Nutrient solution management protocol currently used consists of a modified half Hoagland solution that is changed at 14-day intervals. Reservoirs are refilled with deionized water if the volume of the nutrient solution was reduced to 8 liters or less before the time of solution change. There is the need to recycle and replenish nutrient solution during crop growth, rather than discard at 14 day intervals as previously done, in order to reduce waste. Experiments were conducted in an environmental growth room to examine the effects of container size on the growth of several sweetpotato genotypes grown under a nutrient replenishment protocol. Plants were grown from vine cuttings of 15cm length and were planted in 0.15 x 0.15 x 1.2m growth channels using a closed nutrient film technique system. Nutrient was supplied in a modified half strength Hoagland's solution with a 1:2.4 N:K ratio. Nutrient replenishment protocol consisted of daily water replenishment to a constant volume of 30.4 liters in the small containers and 273.6 liters in the large container. Nutrients were replenished as needed when the EC of the nutrient solution fell below 1200 mhos/cm. The experimental design used was a split-plot with the main plot being container size and genotypes as the subplot. Nine sweetpotato genotypes were evaluated. Results showed no effect of nutrient solution container size on storage root yield, foliage fresh and dry mass, leaf area or vine length. However, plants grown using the large nutrient solution container accumulated more storage root dry mass than those with the small containers. Although plants grown with the smaller containers showed greater water uptake, plant nutrient uptake was lower than with the larger container. All genotypes evaluated showed variation in their responses to all parameters measured.
Sweetpotato was grown to harvest maturity within NASA Johnson Space Center's Variable Pressure Growth Chamber (VPGC) to characterize crop performance for potential use in advanced life support systems as a contributor to food production, air revitalization and resource recovery. Stem cuttings of breeding clone "TU-82-155" were grown hydroponically at a density of 17 plants m(sup -2) using a modified pressure-plate growing system (Patent No. 4860-490, Tuskegee University). Lighting was provided by HPS lamps at a photoperiod of 12h light: 12h dark. The photosynthetic photon flux was maintained at 500, 750 and 1000 micro mol m(sup -2) s(sub -1) during days 1-15, 16-28, 29-119, respectively. Canopy temperatures were maintained at 28 C: light: 22 C:dark. During the light period, relative humidity and carbon dioxide were maintained at 70% and 1200 micro liters l(sup -1), respectively. Nutrient solution was manually adjusted 2 to 4 times per week by addition of 10X concentrated modified half-strength Hoagland nutrient salts and NaOH to return the electrical conductivity and pH to 1.2 mS cm(sup -1) and 6.0, respectively. At 17 weeks (119 days) from transplanting, a total of 56.5 kilograms fresh mass of storage roots (84.1% moisture) were harvested from the 11.2 m(sup 2) chamber, resulting in a yield 5.0 kilograms m(sup -2). Harvest index, based on fresh mass, was 38.6%. Rates of net photosynthesis, dark respiration, transpiration, and ethylene production will be reported.
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Ecosystems worldwide are impacted by altered environment conditions resulting from climate, drought, and land use changes. Gaps in the science knowledge base regarding plant community response to these novel and rapid changes limit both science understanding and management of ecosystems. We describe how CE Technologies have enabled the rapid supply of gap-filling science, development of ecosystem simulation models, and remote sensing assessment tools to provide science-informed, adaptive management methods in the impacted aquatic ecosystem of the California Sacramento-San Joaquin River Delta. The Delta is the hub for California's water, supplying Southern California agriculture and urban communities as well as the San Francisco Bay area. The changes in environmental conditions including temperature, light, and water quality and associated expansion of invasive aquatic plants negatively impact water distribution and ecology of the San Francisco Bay/Delta complex. CE technologies define changes in resource use efficiencies, photosynthetic productivity, evapotranspiration, phenology, reproductive strategies, and spectral reflectance modifications in native and invasive species in response to altered conditions. We will discuss how the CE technologies play an enabling role in filling knowledge gaps regarding plant response to altered environments, parameterization and validation of ecosystem models, development of satellite-based, remote sensing tools, and operational management strategies.
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Microgreens have been identified as a new type of pick-and-eat salad crop that can be utilized in space crop production systems. The majority of traditionally grown leafy green crops can be grown as microgreens, in addition to crops such as legumes, sunflower, buckwheat, most herbs, and corn, presenting hundreds of microgreen crop options. Notably, microgreens are nutrient dense, high in beneficial compounds like antioxidants, Vitamins C and K, and exhibit a variety of desirable flavors and textures.The short growth cycles (7-14 days), low water requirements and volume optimization potential make them a viable option for sustainable production of nutritious and flavorful crops in space.The crop production team at Kennedy Space Center is investigating the food safety aspects of microgreens grown under spaceflight relevant conditions for crew consumption. Microbiological analysis and screening for potential foodborne pathogens was performed on over 20 varieties of microgreens that have demonstrated positive horticultural attributes.Additionally, a comparison of microgreens grown hydroponically under ISS environmental conditions and similar varieties from local markets was completed to collect baseline data on the microbial load on microgreens. In aneffort to improve microgreen quality, strategies to reduce the microbial load were tested, including bulk seed sanitization, harvest age, exposure to high blue light, and post-harvest chemical disinfection. The efficacy of a citric acid-based produce wash currently used for ISS grown produce and 1% H2O2were investigated at different exposure times for reduction in bacterial and fungal counts on a variety of microgreens. Limited log reduction was achieved depending on exposure time.Our testing also demonstrated that seed sanitization impacted microbial load on microgreens and systems.
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As astronauts venture farther from Earth, and for longer periods, the space food system will increase in importance. Crop production can supplement a packaged diet to provide additional nutrients and dietary variety for astronauts. Several unique challenges exist for growth of plants in microgravity and on other planetary surfaces. Testing with the Veggie and Advanced Plant Habitat (APH) chambers on the International Space Station is allowing us to understand the impacts of gravity and spaceflight on crop growth, nutritional content, and food acceptability, and the importance of plants to astronauts living and working away from Earth. Ohalo 3 is a new capability being developed that will pull from both Veggie and APH as we transition from research approaches to an operational space crop production system to enable human exploration of the moon and Mars.
As astronauts venture farther from Earth, and for longer periods, the space food system will increase in importance. Crop production can supplement a packaged diet to provide additional nutrients and dietary variety for astronauts. Several unique challenges exist for growth of plants in microgravity and on other planetary surfaces. Testing with the Veggie and Advanced Plant Habitat (APH) chambers on the International Space Station is allowing us to understand the impacts of gravity and spaceflight on crop growth, nutritional content, and food acceptability, and the importance of plants to astronauts living and working away from Earth. Ohalo III is a new capability being developed that will pull from both Veggie and APH as we transition from research approaches to an operational space crop production system to enable human exploration of the moon and Mars.