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At least 1,063 records · Page 59
All sourcesNASA NTRSDOE OSTI
NASA NTRS2017

Integration of SMAP and SMOS L-Band Observations

Soil Moisture Active Passive (SMAP) mission and the ESA Soil Moisture and Ocean Salinity (SMOS) missions provide brightness temperature and soil moisture estimates every 2-3 days. SMAP brightness temperature observations were compared with SMOS observations at 40 Degrees incidence angle. The brightness temperatures from the two missions are not consistent and have a bias of about 2.7K over land with respect to each other. SMAP and SMOS missions use different retrieval algorithms and ancillary datasets which result in further inconsistencies between the soil moisture products. The reprocessed constant-angle SMOS brightness temperatures were used in the SMAP soil moisture retrieval algorithm to develop a consistent multi-satellite product. The integrated product will have an increased global revisit frequency (1 day) and period of record that would be unattainable by either one of the satellites alone. Results from the development and validation of the integrated product will be presented.

SMOS↗
NASA NTRS2016

Benefit of Modeling the Observation Error in a Data Assimilation Framework Using Vegetation Information Obtained From Passive Based Microwave Data

A primary operational goal of the United States Department of Agriculture (USDA) is to improve foreign market access for U.S. agricultural products. A large fraction of this crop condition assessment is based on satellite imagery and ground data analysis. The baseline soil moisture estimates that are currently used for this analysis are based on output from the modified Palmer two-layer soil moisture model, updated to assimilate near-real time observations derived from the Soil Moisture Ocean Salinity (SMOS) satellite. The current data assimilation system is based on a 1-D Ensemble Kalman Filter approach, where the observation error is modeled as a function of vegetation density. This allows for offsetting errors in the soil moisture retrievals. The observation error is currently adjusted using Normalized Difference Vegetation Index (NDVI) climatology. In this paper we explore the possibility of utilizing microwave-based vegetation optical depth instead.

Vegetation↗
NASA NTRS2017

Improving Our Understanding of Antarctic Sea Ice with NASA's Operation IceBridge and the Upcoming ICESat-2 Mission

Antarctic sea ice is a crucial component of the global climate system. Rapid sea ice production regimes around Antarctica feed the lower branch of the Southern Ocean overturning circulation through intense brine rejection and the formation of Antarctic Bottom Water (e.g., Orsi et al. 1999; Jacobs 2004), while the northward transport and subsequent melt of Antarctic sea ice drives the upper branch of the overturning circulation through freshwater input (Abernathy et al. 2016). Wind-driven trends in Antarctic sea ice (Holland Kwok 2012) have likely increased the transport of freshwater away from the Antarctic coastline, significantly altering the salinity distribution of the Southern Ocean (Haumann et al. 2016). Conversely, weaker sea ice production and the lack of shelf water formation over the Amundsen and Bellingshausen shelf seas promote intrusion of warm Circumpolar Deep Water onto the continental shelf and the ocean-driven melting of several ice shelves fringing the West Antarctic Ice Sheet (e.g., Jacobs et al. 2011; Pritchard et al. 2012; Dutrieux et al. 2014). Sea ice conditions around Antarctica are also increasingly considered an important factor impacting local atmospheric conditions and the surface melting of Antarctic ice shelves (e.g., Scambos et al. 2017). Sea ice formation around Antarctica is responsive to the strong regional variability in atmospheric forcing present around Antarctica, driving this bimodal variability in the behavior and properties of the underlying shelf seas (e.g., Petty et al. 2012; Petty et al. 2014).

Arctic↗
NASA NTRS2017

Growth and Survival of Perchlorate-Reducing Bacteria in Media Containing Elevated Perchlorate Concentrations and UV-C Conditions

Introduction: The identification of perchlorate (ClO4(-)) on Mars has led to the possibility that complete redox couples are available for microbial metabolism in contemporary surface environments. Perchlorate-reducing bacteria (PRB) utilize ClO4(-) and chlorate (ClO3(-)) as terminal electron acceptors due to the high reduction potential. Additionally, ClO4(-) salts have been suggested as a possible source of brines on Mars and spectral evidence indicates that the hydration of ClO4(-) salts in the regolith of Martian is linked to the surface recurring slope lineae (RSL). For these reasons PRB may serve as analog organisms for possible life on Mars. However, there is very little information on the viability of PRB in aqueous environments that contain high levels of perchlorate Microorganisms on or near the surface of Mars, such as in the RSL, would potentially be exposed to high-salinity and high ultraviolet radiation environments. Under these extreme conditions, microorganisms must possess mechanisms for maintaining continued high genome fidelity. To assess possible microbial viability in contemporary Mars analog environments we are investigating the tolerance of two PRB strains in aqueous conditions under high UV-C conditions and high ClO4(-) concentrations.

Bywaters, K. F.↗
NASA NTRS2017

Estrous Cyclicity in Mice During Simulated Weightlessness

Hindlimb unloading (HU) is a rodent model system used to simulate weightlessness experienced in space. However, some effects of this approach on rodent physiology are under-studied, specifically the effects on ovarian estrogen production which drives the estrous cycle. To resolve this deficiency, we conducted a ground-based validation study using the HU model, while monitoring estrous cycles in 16-weeks-old female C57BL6 mice. Animals were exposed to HU for 12 days following a 3 day HU cage acclimation period, and estrous cycling was analyzed in HU animals (n=22), normally loaded HU Cage Pair-Fed controls (CPF; n=22), and Vivarium controls fed ad libitum (VIV; n=10). Pair feeding was used to control for potential nutritional deficits on ovarian function. Vaginal cells were sampled daily in all mice via saline lavage. Cells were dried and stained with crystal violet, and the smears evaluated using established vaginal cytology techniques by two individuals blinded to the animal treatment group. Estrous cyclicity was disrupted in nearly all HU and CPF mice, while those maintained in VIV had an average normal cycle length of 4.8+/- 0.5 days, with all stages in the cycle visibly observed. CPF and HU animals arrested in the diestrous phase, which precedes the pre-ovulatory estrogen surge. Additionally, infection-like symptoms characterized by vaginal discharge and swelling arose in several HU animals, which we suspect was due to an inability of these mice to properly groom themselves, and/or due to the change in the gravity vector relative to the vaginal opening, which prevented drainage of the lavage solution. Pair-feeding resulted in similar weight gains of HU and CPF (1.5% vs 3.0%, respectively). The current results indicate that pair-feeding controlled weight gain and that the HU cage alone influenced estrous cyclicity. Thus, longer acclimation needs to be tested to determine if and when normal estrous cycling resumes in non-loaded mice in HU cages prior to HU testing. Future studies might also examine whether modifications to the vaginal lavage procedure might prevent the onset of the infection-like symptoms, and allow estrous cyclicity to be measured in this model system. Research supported by NNX15AB48G to JST.

Hindlimb Unloading↗
NASA NTRS2017

Estrous Cyclicity of Mice During Simulated Weightlessness

Hindlimb unloading (HU) is a rodent model system used to simulate weightlessness experienced in space. However, some effects of this approach on rodent physiology are under-studied, specifically the effects on ovarian estrogen production which drives the estrous cycle. To resolve this deficiency, we conducted a ground-based validation study using the HU model, while monitoring estrous cycles in 16-weeks-old female C57BL6 mice. Animals were exposed to HU for 12 days following a 3 day HU cage acclimation period, and estrous cycling was analyzed in HU animals (n22), normally loaded HU Cage Pair-Fed controls (CPF; n22), and Vivarium controls fed ad libitum (VIV; n10). Pair feeding was used to control for potential nutritional deficits on ovarian function. Vaginal cells were sampled daily in all mice via saline lavage. Cells were dried and stained with crystal violet, and the smears evaluated using established vaginal cytology techniques by two individuals blinded to the animal treatment group. Estrous cyclicity was disrupted in nearly all HU and CPF mice, while those maintained in VIV had an average normal cycle length of 4.8 0.5 days, with all stages in the cycle visibly observed. CPF and HU animals arrested in the diestrous phase, which precedes the pre-ovulatory estrogen surge. Additionally, infection-like symptoms characterized by vaginal discharge and swelling arose in several HU animals, which we suspect was due to an inability of these mice to properly groom themselves, andor due to the change in the gravity vector relative to the vaginal opening, which prevented drainage of the lavage solution. Pair-feeding resulted in similar weight gains of HU and CPF (1.5 vs 3.0, respectively). The current results indicate that pair-feeding controlled weight gain and that the HU cage alone influenced estrous cyclicity. Thus, longer acclimation needs to be tested to determine if and when normal estrous cycling resumes in non-loaded mice in HU cages prior to HU testing. Future studies might also examine whether modifications to the vaginal lavage procedure might prevent the onset of the infection-like symptoms, and allow estrous cyclicity to be measured in this model system.

fertilit↗
NASA NTRS2018

The Ocean Carbon States Database: A Proof-of-Concept Application of Cluster Analysis in the Ocean Carbon Cycle

In this paper, we present a database of the basic regimes of the carbon cycle in the ocean, the 'ocean carbon states', as obtained using a data mining/pattern recognition technique in observation-based as well as model data. The goal of this study is to establish a new data analysis methodology, test it and assess its utility in providing more insights into the regional and temporal variability of the marine carbon cycle. This is important as advanced data mining techniques are becoming widely used in climate and Earth sciences and in particular in studies of the global carbon cycle, where the interaction of physical and biogeochemical drivers confounds our ability to accurately describe, understand, and predict CO2 concentrations and their changes in the major planetary carbon reservoirs. In this proof-of-concept study, we focus on using well-understood data that are based on observations, as well as model results from the NASA Goddard Institute for Space Studies (GISS) climate model. Our analysis shows that ocean carbon states are associated with the subtropical-subpolar gyre during the colder months of the year and the tropics during the warmer season in the North Atlantic basin. Conversely, in the Southern Ocean, the ocean carbon states can be associated with the subtropical and Antarctic convergence zones in the warmer season and the coastal Antarctic divergence zone in the colder season. With respect to model evaluation, we find that the GISS model reproduces the cold and warm season regimes more skillfully in the North Atlantic than in the Southern Ocean and matches the observed seasonality better than the spatial distribution of the regimes. Finally, the ocean carbon states provide useful information in the model error attribution. Model air-sea CO2 flux biases in the North Atlantic stem from wind speed and salinity biases in the subpolar region and nutrient and wind speed biases in the subtropics and tropics. Nutrient biases are shown to be most important in the Southern Ocean flux bias.

carbon cycle↗
NASA NTRS2018

Satellite Sensor Requirements for Monitoring Essential Biodiversity Variables of Coastal Ecosystems

The biodiversity and high productivity of coastal terrestrial and aquatic habitats are the foundation for important benefits to human societies around the world. These globally distributed habitats need frequent and broad systematic assessments, but field surveys only cover a small fraction of these areas. Satellite-based sensors can repeatedly record the visible and near-infrared reflectance spectra that contain the absorption, scattering, and fluorescence signatures of functional phytoplankton groups, colored dissolved matter, and particulate matter near the surface ocean, and of biologically structured habitats (floating and emergent vegetation, benthic habitats like coral, seagrass, and algae). These measures can be incorporated into Essential Biodiversity Variables (EBVs), including the distribution, abundance, and traits of groups of species populations, and used to evaluate habitat fragmentation. However, current and planned satellites are not designed to observe the EBVs that change rapidly with extreme tides, salinity, temperatures, storms, pollution, or physical habitat destruction over scales relevant to human activity. Making these observations requires a new generation of satellite sensors able to sample with these combined characteristics: (1) spatial resolution on the order of 30 to 100-m pixels or smaller; (2) spectral resolution on the order of 5 nm in the visible and 10 nm in the short-wave infrared spectrum (or at least two or more bands at 1,030, 1,240, 1,630, 2,125, and/or 2,260 nm) for atmospheric correction and aquatic and vegetation assessments; (3) radiometric quality with signal to noise ratios (SNR) above 800 (relative to signal levels typical of the open ocean), 14-bit digitization, absolute radiometric calibration less than 2%, relative calibration of 0.2%, polarization sensitivity less than 1%, high radiometric stability and linearity, and operations designed to minimize sunglint; and (4) temporal resolution of hours to days. We refer to these combined specifications as H4 imaging. Enabling H4 imaging is vital for the conservation and management of global biodiversity and ecosystem services, including food provisioning and water security. An agile satellite in a 3-d repeat low-Earth orbit could sample 30-km swath images of several hundred coastal habitats daily. Nine H4 satellites would provide weekly coverage of global coastal zones. Such satellite constellations are now feasible and are used in various applications.

aquatic↗
NASA NTRS2017

Localization of L-Band RFI Sources from SMAP Data

RFI (Radio-Frequency Interference) in the 1400-1427 MHz band degrades the quality of measurements made by satellite missions such as SMAP (Soil Moisture Active/Passive), Aquarius and SMOS (Soil Moisture and Ocean Salinity). A technique is presented here to estimate the location on the ground of RFI sources using SMAP measurements. The results of this technique have been validated against data derived by other means.

Soldo, Yan↗
NASA NTRS2018

Global-scale Evaluation of SMAP, SMOS and ASCAT Soil Moisture Products Using Triple Collocation

Global-scale surface soil moisture products are currently available from multiple remote sensing platforms. Footprint-scale assessments of these products are generally restricted to limited number of densely-instrumented validation sites. However, by taking active and passive soil moisture products together with a third independent soil moisture estimates via land surface modeling, triple collocation (TC) can be applied to estimate the correlation metric of satellite soil moisture products (versus an unknown ground truth) over a quasi-global domain. Here, an assessment of Soil Moisture Active Passive (SMAP), Soil Moisture Ocean Salinity (SMOS) and Advanced SCATterometer (ASCAT) surface soil moisture retrievals via TC is presented. Considering the potential violation of TC error assumptions, the impact of active-passive and satellite-model error cross correlations on the TC-derived inter-comparison results is examined at in situ sites using quadruple collocation analysis. In addition, confidence intervals for the TC-estimated correlation metric are constructed from moving-block bootstrap sampling designed to preserve the temporal persistence of the original (unevenly-sampled) soil moisture time-series. This study is the first to apply TC to obtain a robust global-scale cross-assessment of SMAP, SMOS and ASCAT soil moisture retrieval accuracy in terms of anomaly temporal correlation. Our results confirm the overall advantage of SMAP (with a global average anomaly correlation of 0.76) over SMOS (0.66) and ASCAT (0.63) that has been established in several recent regional, ground-based studies. SMAP is also the best-performing product over the majority of applicable land pixels (52%), although SMOS and ASCAT each shows advantage in distinct geographic regions.

global-scale↗
NASA NTRS2018

Aquarius Final Release Product and Full Range Calibration of L-band Radiometers

Aquarius final product V5.0 has been released. The dataset includes close to four years of global radiometric measurements at L-band. The mission's objective was to monitor sea surface salinity, but other applications of its data over land and the cryosphere have been developed. For this reason, it is important to have accurate calibration over the full range of antenna temperatures from natural targets. It is also needed in order to combine Aquarius measurements with other L-band sensors. Aquarius calibration is strongly focused on the ocean. We present a research product which is part of the final release and aims at producing an accurate calibration from the low end (celestial sky) to the high end (land and ice) of the brightness temperature scale. We calibrate the Aquarius radiometers using measurements over the Sky and oceans and assess the new calibration using measurements over land.

Radiometry↗
NASA NTRS2018

Radio Frequency Interference (RFI) Products on the Aquarius Website

Aquarius has produced maps of salinity by measuring Earth’s natural emissions at L-band. However, measurements made by its instruments are affected by the presence of Radio Frequency Interference (RFI). For this reason, RFI detection algorithms had been implemented, both for the radiometer and the scatterometer, in order to reduce the impact of RFI on science data. In an effort to improve understanding of L-band RFI, the Aquarius mission has generated a new series of products. This contribution presents how these products were produced as well as the information that they contain. These products will be available starting at the end of January 2018 on the Aquarius website.

Radio Frequency Interference↗
NASA NTRS2018

Science Service Status 2019 World Radiocommunication Conference

Science missions such as Earth-exploration and remote sensing thatuse frequencies allocated for Earth Exploration-Satellite Service(EESS) provide valuable information about, for example: (1) Air quality monitoring (2) Soil moisture mapping (3) Sensing sea salinity (4) Weather forecasting and climate models (winds, storms, clouds).Another set of science missions use Space Research Service (SRS)allocations. These space research missions can support: (1) On orbit research that for example can help in the creation of new drugsor the impact of the space environment on satellites (2) Scientific information about the universe – stars, planetary science, solarsystem, and the future evolution of the universe.

Roura, Corali↗
NASA NTRS2018

Integration of SMAP and SMOS Observations

Soil Moisture Active Passive (SMAP) mission and the Soil Moisture and Ocean Salinity (SMOS) missions provide brightness temperature and soil moisture estimates every 2-3 days. SMAP brightness temperature observations were compared with SMOS observations at 40o incidence angle. The brightness temperatures from the two missions are not consistent. SMAP observations show a warmer TB bias (about 1.27 K: V pol and 0.62 K: H pol) as compared to SMOS. SMAP and SMOS missions use different retrieval algorithms and ancillary datasets which result in further inconsistencies between their soil moisture products. The reprocessed constant-angle SMOS brightness temperatures were used in the SMAP soil moisture retrieval algorithm to develop a consistent multi-satellite product. The integrated product has an increased global revisit frequency (1 day) and period of record that is unattainable by either one of the satellites alone. Results from the development and validation of the integrated soil moisture product will be presented.

passive microwave↗
NASA NTRS2018

Assimilation of Satellite Derived Soil Moisture Profiles into a Crop Modeling System for Robust Yield Estimates

Soil Moisture Measurement - Remote Sensing. - Microwave (MW) Remote Sensing: Physically based and quantitative in nature; Based on difference in dielectric constant; Coarse spatial resolution 25-40 km; Shallow SM estimation depth 0-5 cm (approx.); All weather capabilities (e.g. Advanced Microwave Scanning Radiometer - Earth Observing System (AMSR-E), Soil Moisture and Ocean Salinity (SMOS), Soil Moisture Active Passive (SMAP) etc.) - Thermal Infrared (TIR) Remote Sensing: Indirect SM retrieval through energy flux estimations; Relatively better spatial resolution 1-10 km; Root-zone moisture retrieval capability; Can not penetrate through clouds, hence data gaps (e.g. Surface Energy Balance Algorithm for Land (SEBAL), Atmospheric Land Exchange Inverse (ALEXI) etc.)

SERVIR↗
NASA NTRS2017

The Don Edwards San Francisco Bay National Wildlife Refuge: A Natural Laboratory for Federal Agencies and Partners

The Don Edwards San Francisco Bay National Wildlife Refuge encompasses a rich mosaic of habitats and is a fecund and convenient field site for Earth science research, comprised of tidal salt marsh, open ponds, shallow water, mudflat habitat and encompassing a variety of tide, salinity, elevation, slope, and other conditions. Proximity to the NASA Ames Research Center and overlap with the center's UAS (unmanned aircraft systems) test area presents the opportunity to create a unique natural laboratory that can incubate and support a variety of research and benefit the Refuge in monitoring wildlife changes and habitat evolution over time.

Unmanned Airborne Vehicle↗
NASA NTRS2018

Impacts of NASA's Earth Observations on Subseasonal and Seasonal Forecasts

NASA's suite of Earth-observing satellites provides a unique view of many processes on Earth, with relevance on timescales ranging from hours to weeks and even years. NASA's observations span all parts of the Earth system: atmospheric, ocean, land and cryosphere, and include physical, chemical and biological components. This presentation explores use of NASA observations in extended-range prediction, from many days to months, using the Goddard Earth Observing System (GEOS) assimilation and predictive modeling capabilities. The skill of a weather forecast is linked to the fidelity of the initialization process (data assimilation) and the realistic representation of "fast" physical processes in the model. At longer time horizons, the slower "feedback" processes in the Earth System begin to take a more prominent role in the accuracy of the forecast. Simultaneously, forecast-skill attribution transitions away from feature-based metrics that emphasize smaller scales (e.g., representations of fronts and vortices) to metrics that emphasize the statistical distributions of large-scale features (e.g., ENSO diagnostics and teleconnections). This presentation will summarize studies performed using the GEOS-S2S (subseasonal to seasonal) system that explore the impacts of NASA observations on the fidelity of the forecasts. The GEOS-S2S system is configured for the atmosphere-ocean-land-ice model and is initialized using in-situ and space-based observations, including atmospheric aerosols and ozone which are not typically analyzed in such systems. The GEOS-S2S model routinely includes aerosol feedbacks, which systematically impact the realism of the forecasts, providing a first example of how suitable NASA observations impact the performance of the GEOS-S2S system. Studies in which a stratospheric chemistry module is activated in the GEOS-S2S system allow the impacts of ozone radiative feedbacks to be isolated. Space-based observations of sea-surface temperature and altimetry are routinely analyzed for the initialization of the GEOS-S2S system; recent advances allow the use of NASA's sea-surface salinity data, which are shown to impact the long-range skill of the forecasts.

Pawson, Steven↗
NASA NTRS2017

Zoledronate Prevents Simulated Weightlessness-Induced Bone Loss in the Cancellous Compartment While Blunting the Efficacy of a Mechanical Loading Countermeasure

Astronauts using high-force resistance training while weightless show a high-turnover remodeling state within the skeletal system, with resorption and formation biomarkers being elevated. One countermeasure for the skeletal health of astronauts includes an antiresorptive of the bisphosphonate (BP) drug class. We asked, does the combination of an anti-resorptive and high-force exercise during weightlessness have negative effects on bone remodeling and strength? In this study, we developed an integrated model to mimic the mechanical strain of exercise via cyclical loading (CL) in mice treated with the BP Zoledronate (ZOL) combined with hind limb unloading (HU) to simulate weightlessness. We hypothesized that ZOL prevents structural degradation from simulated weightlessness and that CL and ZOL interact to render CL less effective. Thirty-two C57BL/6 mice (male, 16 weeks old, n=8/group) were exposed to 3 weeks of either HU or normal ambulation (NA). Cohorts of mice received one subcutaneous injection of ZOL (45μg/kg), or saline vehicle (VEH), prior to the start of HU. The right tibia was axially compressed in vivo 60x/day to 9N (+1200μstrain on the periosteal surface) and repeated 3x/week during HU. Left tibiae served as a within subject, non-compressed control. Ex vivo μCT was performed on all subjects to determine cancellous and cortical architectural parameters. Static and dynamic histomorphometry were carried out for the left and right tibiae to determine osteoclast- and osteoblast relevant surfaces. Further, micro damage was assessed in select groups by basic-fuchsin staining to test whether CL had an effect. For all assays, a multivariate (2x2x2) ANCOVA model was used to account for body weight changes. Additionally, for the tibiae, we incorporated a random effect for the subject (hence, a mixed model) to account for observations of both left and right tibiae within each subject. P < 0.05 was considered significant. In the cancellous compartment of the proximal tibial metaphysis, we observed a main effect from each independent variable, as determined by structural and histomorphometric assays. Specifically, as expected, ZOL showed an increase in the cancellous bone volume to total volume fraction (BV/TV, +32%) and trabecular number (+18%) compared to the VEH. As expected, ZOL decreased osteoclast surface (OC/BS) by -45% compared to VEH. Surprisingly, ZOL reduced mineralizing surface (MS/BS) and bone formation rate (BFR), indicators of osteoblast activity, by -40% and -54%, respectively, compared to VEH. Altogether, ZOL-treated mice displayed a low turnover state of remodeling in the metaphysis. In the context of skeletal aging, we speculate that ZOL prevented age-related cancellous strut loss during the experiment. As a main effect, as expected, HU decreased BV/TV by - 31% via reductions in both trabecular thickness (-11%) and number (-22%) compared to NA controls. Additionally, HU decreased MS/BS by -38% and bone formation rate (BFR) by -50% compared to NA controls. Altogether, these data are consistent with structural degradation resulting from imbalanced remodeling that favors resorption. As a main effect, CL increased BV/TV by +15% via increased trabecular thickness (+12%) compared to the noncompressed limb. As expected, CL increased MS/BS (+20%) and BFR (+24%), indicating osteoblast mineralization contributed to bone gains. These data show that CL provided an anabolic stimulus to the cancellous tissue. We observed unique interactions in ZOL*CL and HU*CL. First, ZOL prevented CL-induced increases in BV/TV and trabecular number, as compared to VEH. In the context of skeletal aging, these data suggest no added benefit from CL in the ZOL-treated mice. Interestingly, no microdamage was observed in mice that were unloaded and treated with ZOL (independent of CL). Secondly, HU prevented CL-induced increases in BFR, as compared to NA controls. These data suggest that either exercise is less effective or the kinetics of formation are slower during simulated weightlessness. Osteoclast surface was unchanged by either treatment. Thus, in contrast to exercising astronauts, these data do not suggest a high-turnover state in the metaphysis. To assess mechanical properties as a function of HU or ZOL, we tested the left femur in three-point bending ex vivo. As expected, HU decreased stiffness (-30%) compared to NA, and ZOL increased stiffness compared to VEH (+28%). Interestingly, HU increased the post-yield displacement, related to collagenous tensile loading, compared to NA (+20%). ZOL increased yield force (+11%) and ultimate force (+17%), which seems to explain the significant effect of ZOL increasing total energy (work-to-fracture, +15%), while not affecting the post yield displacement. Taken together, ZOL did not have detrimental affect on mechanical properties. Our integrated model simulates the combination of weightlessness, exercise-induced mechanical strain, and anti-resorptive treatment that astronauts experience during space missions. We conclude that Zoledronate was an effective countermeasure against weightlessness-induced bone loss, though zoledronate, as well as weightlessness, rendered exercise-related mechanical loading less effective.

bone↗