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At least 1,063 records · Page 59
All sourcesNASA NTRSDOE OSTI
DOE OSTI2023

Correlative montage parallel array cryo-tomography for in situ structural cell biology

Abstract Imaging large fields of view while preserving high-resolution structural information remains a challenge in low-dose cryo-electron tomography. Here we present robust tools for montage parallel array cryo-tomography (MPACT) tailored for vitrified specimens. The combination of correlative cryo-fluorescence microscopy, focused-ion-beam milling, substrate micropatterning, and MPACT supports studies that contextually define the three-dimensional architecture of cells. To further extend the flexibility of MPACT, tilt series may be processed in their entirety or as individual tiles suitable for sub-tomogram averaging, enabling efficient data processing and analysis.

59 BASIC BIOLOGICAL SCIENCES↗
NASA NTRS2009

Improved Scanners for Microscopic Hyperspectral Imaging

Improved scanners to be incorporated into hyperspectral microscope-based imaging systems have been invented. Heretofore, in microscopic imaging, including spectral imaging, it has been customary to either move the specimen relative to the optical assembly that includes the microscope or else move the entire assembly relative to the specimen. It becomes extremely difficult to control such scanning when submicron translation increments are required, because the high magnification of the microscope enlarges all movements in the specimen image on the focal plane. To overcome this difficulty, in a system based on this invention, no attempt would be made to move either the specimen or the optical assembly. Instead, an objective lens would be moved within the assembly so as to cause translation of the image at the focal plane: the effect would be equivalent to scanning in the focal plane. The upper part of the figure depicts a generic proposed microscope-based hyperspectral imaging system incorporating the invention. The optical assembly of this system would include an objective lens (normally, a microscope objective lens) and a charge-coupled-device (CCD) camera. The objective lens would be mounted on a servomotor-driven translation stage, which would be capable of moving the lens in precisely controlled increments, relative to the camera, parallel to the focal-plane scan axis. The output of the CCD camera would be digitized and fed to a frame grabber in a computer. The computer would store the frame-grabber output for subsequent viewing and/or processing of images. The computer would contain a position-control interface board, through which it would control the servomotor. There are several versions of the invention. An essential feature common to all versions is that the stationary optical subassembly containing the camera would also contain a spatial window, at the focal plane of the objective lens, that would pass only a selected portion of the image. In one version, the window would be a slit, the CCD would contain a one-dimensional array of pixels, and the objective lens would be moved along an axis perpendicular to the slit to spatially scan the image of the specimen in pushbroom fashion. The image built up by scanning in this case would be an ordinary (non-spectral) image. In another version, the optics of which are depicted in the lower part of the figure, the spatial window would be a slit, the CCD would contain a two-dimensional array of pixels, the slit image would be refocused onto the CCD by a relay-lens pair consisting of a collimating and a focusing lens, and a prism-gratingprism optical spectrometer would be placed between the collimating and focusing lenses. Consequently, the image on the CCD would be spatially resolved along the slit axis and spectrally resolved along the axis perpendicular to the slit. As in the first-mentioned version, the objective lens would be moved along an axis perpendicular to the slit to spatially scan the image of the specimen in pushbroom fashion.

Mao, Chengye↗
NASA NTRS2019

The Solar Neutron TRACking (SONTRAC) Instrument for the Detection of Fast Neutrons

The detection of fast neutrons has important applications in several fields including solar, Geospace and planetary physics. Neutrons are challenging to detect and measurements of them typically suffer from high background rates. High-energy neutrons (>50 MeV) pose even more challenges, because the traditional double-scatter technique based on a time-of-flight (ToF) measurement is limited by short flight paths and small detector sizes characteristic of small satellite platforms. It is now possible to perform high-energy neutron measurements inside a large monolithic detector by imaging the recoil proton tracks, thus eliminating the need for a measure of the time-of-flight. The concept is based on a spectrometer assembled from numerous thin hydrogenous scintillating fibers that allow ionization track imaging. Fine grained readout is now possible with arrays of1-mm pitch silicon photomultipliers (SiPMs). The Solar Neutron TRACking (SONTRAC) instrument, equipped with scintillating fibers readout with SiPMs sensors, provides high-resolution, fine grained, imaging of fast (between 20-200 MeV) neutron scatters in a compact, low-power design ideal for small satellite(and aircraft) platforms. We discuss below applications of this technology and performance characteristics of the prototype SONTRAC instrument.

de Nolfo, Georgia A.↗
NASA NTRS1987

The AXAF CCD Imaging Spectrometer Experiment (ACIS)

The ACIS experiment consists of an array of charge coupled device (CCD) chips placed at the focus of the AXAF mirror assembly. The instrument offers a powerful combination of the best qualities of the entire generation of Einstein Observatory detectors. The pixel size is well matched to the resolution of the telescope, while the high quantum efficiency nondispersive spectroscopy capability means that every object imaged will produce a moderate resolution spectrum. In conjunction with the objective gratings the energy resolution increases to 1000 with a loss of efficiency of only 50 percent. While the great capability of ACIS will naturally make it attractive for a wide range of AXAF investigations, ACIS has a particular advantage in fields where it can simultaneously acquire imaged spectra of many objects. These include compact star clusters, star formation regions, the nuclear regions of nearby galaxies, entire galaxies in the Virgo Cluster and the entire Coma Cluster.

Nousek, J. A.↗
NASA NTRS2012

NASA Tech Briefs, July 2012

Topics covered include: Instrument Suite for Vertical Characterization of the Ionosphere-Thermosphere System; Terahertz Radiation Heterodyne Detector Using Two-Dimensional Electron Gas in a GaN Heterostructure; Pattern Recognition Algorithm for High-Sensitivity Odorant Detection in Unknown Environments; Determining Performance Acceptability of Electrochemical Oxygen Sensors; Versatile Controller for Infrared Lamp and Heater Arrays; High-Speed Scanning Interferometer Using CMOS Image Sensor and FPGA Based on Multifrequency Phase-Tracking Detection; Ultra-Low-Power MEMS Selective Gas Sensors; Compact Receiver Front Ends for Submillimeter-Wave Applications; Dynamically Reconfigurable Systolic Array Accelerator; Blocking Losses With a Photon Counter; Motion-Capture-Enabled Software for Gestural Control of 3D Mod; Orbit Software Suite; CoNNeCT Baseband Processor Module Boot Code SoftWare (BCSW); Trajectory Software With Upper Atmosphere Model; ALSSAT Version 6.0; Employing a Grinding Technology to Assess the Microbial Density for Encapsulated Organisms; Demonstration of Minimally Machined Honeycomb Silicon Carbide Mirrors; Polyimide Aerogel Thin Films; Nanoengineered Thermal Materials Based on Carbon Nanotube Array Composites; Composite Laminate With Coefficient of Thermal Expansion Matching D263 Glass; Robust Tensioned Kevlar Suspension Design; Focal Plane Alignment Utilizing Optical CMM; Purifying, Separating, and Concentrating Cells From a Sample Low in Biomass; Virtual Ultrasound Guidance for Inexperienced Operators; Beat-to-Beat Blood Pressure Monitor; Non-Contact Conductivity Measurement for Automated Sample Processing Systems; An MSK Radar Waveform; Telescope Alignment From Sparsely Sampled Wavefront Measurements Over Pupil Subapertures; Method to Remove Particulate Matter from Dusty Gases at Low Pressures; Terahertz Quantum Cascade Laser With Efficient Coupling and Beam Profile; Measurement Via Optical Near-Nulling and Subaperture Stitching; 885-nm Pumped Ceramic Nd:YAG Master Oscillator Power Amplifier Laser System; Airborne Hyperspectral Imaging System; Heat Shield Employing Cured Thermal Protection Material Blocks Bonded in a Large-Cell Honeycomb Matrix; and Asymmetric Supercapacitor for Long-Duration Power Storage.

Source record↗
NASA NTRS1994

NIFTE: The Near Infrared Faint-Object Telescope Experiment

The high sensitivity of large format InSb arrays can be used to obtain deep images of the sky at 3-5 micrometers. In this spectral range cool or highly redshifted objects (e.g. brown dwarfs and protogalaxies) which are not visible at shorter wavelengths may be observed. Sensitivity at these wavelengths in ground-based observations is severly limited by the thermal flux from the telescope and from the earth's atmosphere. The Near Infrared Faint-Object Telescope Experiment (NIFTE), a 50 cm cooled rocket-borne telescope combined with large format, high performance InSb arrays, can reach a limiting flux less than 1 micro-Jy(1-sigma) over a large field-of-view in a single flight. In comparison, the Infrared Space Observatory (ISO) will require days of observation to reach a sensitivity more than one order of magnitude worse over a similar area of the sky. The deep 3-5 micrometer images obtained by the rocket-borne telescope will assist in determining the nature of faint red objects detected by ground-based telescopes at 2 micrometers, and by ISO at wavelengths longer than 5 micrometers.

Bock, James J.↗
NASA NTRS1983

Short wavelength infrared hybrid focal plane arrays

The employment of area focal plane arrays (FPA) has made it possible to obtain second generation infrared imaging systems with high resolution and sensitivity. The Short Wavelength Infrared (SWIR) region (1-2.5 microns) is of importance for imaging objects at high temperature and under conditions of reflected sunlight. The present investigation is concerned with electrooptical characterization results for 32 x 32 SWIR detector arrays and FPAs which are suitable for use in a prototype imaging spectrometer. The employed detector material is Hg(1-x)Cd(x)Te grown by liquid phase epitaxy on a CdTe transparent substrate. Attention is given to details of processing, the design of the detector array, the multiplexer, the fabrication of the hybrid FPA, and aspects of performance.

Vural, K.↗
NASA NTRS1986

Flexible tactile sensing arrays for robotics: Architectural robustness and yield considerations

No tactile sensor array developed to date fills all, nor even most, of the requirements of: (1) many sensor elements to minimize tactile imaging time, (2) close spacing of small sensing elements for fine spatial discrimination, (3) ability to be molded to a convex fingertip surface for taction on concave surfaces, (4) ability to sense both force and temperature in order to fully mimic biological touch, (5) tolerance to overscale forces without damage, and (6) graceful failure in use, i.e., functional survival of most of the array when part of it is damaged, well. Several investigators have recently developed tactile sensing arrays suitable for imaging shapes on hard flat surfaces, but not well suited for taction on curved surfaces. Others have developed single tactile sensors suitable for incorporation into arrays, which will probably prove uneconomical when large numbers of sensors are needed. Still others have developed flexible capacitive arrays suitable for use on curved surfaces but incapable of obtaining temperature or proximity data. A technology is being developed herein suitable to the achievement of all of the above goals, and to incrementally demonstrate useful steps toward those goals.

Barth, Phillip W.↗
DOE OSTI2024

Optimized Viewing Techniques to Minimize Radiation Damage From X-ray Imaging Systems

X-ray inspection of ball grid arrays (BGAs) is typically performed at one or more viewing angles to examine adhesion sites for errors such as voids, joint cracking, or head-in-pillow. During this inspection process, the cir cuit board assembly is subject to ionizing radiation exposure, which can cause trapped charge within oxide layers of semiconductor devices. Some x-ray machines allow for programmable inspection routines, which could be used to optimize radiation exposure to semiconductor components. Using Monte Carlo methods, x-ray inspection of a BGA was simulated to determine a range of acceptable viewing angles. Dose rates to circuit board components were estimated at each inspection angle to determine the view resulting in optimized radiation exposure. Results showed that for each BGA, the maximum unobstructed viewing times without exceeding a 5 Gy dose limit to a single part ranged from 82 to 94 minutes. Using a radiation cost function method, optimized viewing across all components was found. Here, it was observed that for a consistent dose limit applied to silicon-based components, performing inspection with BGAs facing the x-ray source was optimal. A third method was applied, assigning individual dose limits based on empirical data from the NASA Goddard Space Flight Center radiation database. This method showed that optimized viewing maximizes the distance between the radiation source and highly sensitive components. It was also observed that cumulative effects from viewing two BGAs will influence viewing angles, causing the optimal view of one BGA to exist nearly 180° from the other.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗
NASA NTRS1984

Plans for a fast image recording system at ESO

A 256 diode-array will be installed as detector on the ESO OPTRONICS S-3000 measuring machine in order to increase the acquisition rate. A high intensity LED will be used as light source in a pulse mode. The data will be stored on a random access mass storage device as density values for later education. The scanning time for a 30 cm x 30 cm plate with a step size of 10 micron will be less than 10 hours while the dynamic range of the data is expected to be 2.5 density units with an offset of at least 1 unit.

Grosboel, P. J.↗
NASA NTRS1987

Simultaneous SMM flat crystal spectrometer and Very Large Array observations of solar active regions

High-resolution images of the quiescent emission from two solar active regions at 20 cm (VLA) and soft X-ray (SMM FCS) wavelengths are compared. There are regions where the X-ray coronal loops have been completely imaged at 20 cm wavelength. In other regions, the X-ray radiation was detected without detectable 20 cm radiation, and vice versa. The X-ray data were used to infer average electron temperatures of about 3-million K and average electron densities of about 2.5 x 10 to the 9th/cu cm for the X-ray emitting plasma in the two active regions. The thermal bremsstrahlung of the X-ray emitting plasma is optically thin at 20 cm wavelength. The 20 cm brightness temperatures were always less than T(e), which is consistent with optically thin bremsstrahlung. The low T(B) can be explained if a higher, cooler plasma covers the hotter X-ray emitting plasma. Thermal gyroresonance radiation must account for the intense 20 cm radiation near and above sunspots where no X-ray radiation is detected.

Lang, Kenneth R.↗
NASA NTRS1990

Near-infrared imaging of interacting galaxies

A sample of 22 interacting galaxies has been imaged in the J and K band using an array camera with resolution of 0.76 arcsec/pixel, and an initial description of the resulting data is provided. The near-IR images show the location of previously invisible nuclei in some galaxies, and in others show that features once thought to be nuclei may actually be 'hot spots' of star formation activity. Analysis of the spatial distribution of IR colors shows that virtually all the galaxies become redder toward their nuclei. It is concluded that most of this effect is due to an increase in the amount of dust in the nuclei, with smaller contributions from metallicity and population gradients.

Bushouse, Howard A.↗
NASA NTRS1993

Constructing An Image From Nonuniform-Scan Data

Algorithm performs maximum-likelihood construction of image of scene scanned nonuniformly by linear array of photodetectors, responses of which unequal. Developed to postprocess data acquired by scanning telescope aboard Infrared Astronomical Satellite (IRAS), also adaptable to generation of high-resolution imagery from data acquired in other nonuniform-scanning applications. Achievable resolution not limited by finite size of detectors, and increases with density of scans. With sufficiently dense scans, one obtains resolution beyond diffraction limit of imaging optics.

Aumann, Hartmut H.↗
NASA NTRS1991

Monolithic microwave integrated circuits for sensors, radar, and communications systems; Proceedings of the Meeting, Orlando, FL, Apr. 2-4, 1991

Consideration is given to MMICs for airborne phased arrays, monolithic GaAs integrated circuit millimeter wave imaging sensors, accurate design of multiport low-noise MMICs up to 20 GHz, an ultralinear low-noise amplifier technology for space communications, variable-gain MMIC module for space applications, a high-efficiency dual-band power amplifier for radar applications, a high-density circuit approach for low-cost MMIC circuits, coplanar SIMMWIC circuits, recent advances in monolithic phased arrays, and system-level integrated circuit development for phased-array antenna applications. Consideration is also given to performance enhancement in future communications satellites with MMIC technology insertion, application of Ka-band MMIC technology for an Orbiter/ACTS communications experiment, a space-based millimeter wave debris tracking radar, low-noise high-yield octave-band feedback amplifiers to 20 GHz, quasi-optical MESFET VCOs, and a high-dynamic-range mixer using novel balun structure.

Leonard, Regis F.↗
NASA NTRS1988

Measurement of the Landsat Thematic Mapper modulation transfer function using an array of point sources

This paper presents a method for measuring the Thematic Mapper (TM) imaging system point spread function (PSF) using TM imagery or a specially constructed target consisting of a two-dimensional array of approximate point sources of known dimensions and radiometric qualities. The target allows 16 separate point sources to be imaged simultaneously by the TM. The point sources were carefully placed on the ground so that their relative positions were known. Owing to sample-scene phasing, each imaged point source exhibits a different amount of blur in the digital image. The target pixels may then be recombined according to their known relative positions to form a single, sampled, nonaliased imaging system PSF. The modulation transfer function is then obtained as the modulus of the discrete Fourier transform of the PSF.

Rauchmiller, Robert F., Jr.↗
NASA NTRS2009

Scanning Miniature Microscopes without Lenses

The figure schematically depicts some alternative designs of proposed compact, lightweight optoelectronic microscopes that would contain no lenses and would generate magnified video images of specimens. Microscopes of this type were described previously in Miniature Microscope Without Lenses (NPO - 20218), NASA Tech Briefs, Vol. 22, No. 8 (August 1998), page 43 and Reflective Variants of Miniature Microscope Without Lenses (NPO 20610), NASA Tech Briefs, Vol. 26, No. 9 (September 1999), page 6a. To recapitulate: In the design and construction of a microscope of this type, the focusing optics of a conventional microscope are replaced by a combination of a microchannel filter and a charge-coupled-device (CCD) image detector. Elimination of focusing optics reduces the size and weight of the instrument and eliminates the need for the time-consuming focusing operation. The microscopes described in the cited prior articles contained two-dimensional CCDs registered with two-dimensional arrays of microchannels and, as such, were designed to produce full two-dimensional images, without need for scanning. The microscopes of the present proposal would contain one-dimensional (line image) CCDs registered with linear arrays of microchannels. In the operation of such a microscope, one would scan a specimen along a line perpendicular to the array axis (in other words, one would scan in pushbroom fashion). One could then synthesize a full two-dimensional image of the specimen from the line-image data acquired at one-pixel increments of position along the scan. In one of the proposed microscopes, a beam of unpolarized light for illuminating the specimen would enter from the side. This light would be reflected down onto the specimen by a nonpolarizing beam splitter attached to the microchannels at their lower ends. A portion of the light incident on the specimen would be reflected upward, through the beam splitter and along the microchannels, to form an image on the CCD. If the nonpolarizing beam splitter were replaced by a polarizing one, then the specimen would be illuminated by s-polarized light. Upon reflection from the specimen, some of the s-polarized light would become p-polarized. Only the p-polarized light would contribute to the image on the CCD; in other words, the image would contain information on the polarization rotating characteristic of the specimen.

Wang, Yu↗
NASA NTRS1995

Airborne spectrograph for the thermal IR: Broadband Array Spectrograph System

Spectroscopic studies in the 'fingerprint' region of the thermal IR from 3 to 14 microns of celestial dust components and the overall energy distribution of the sources are best served by moderate spectral resolution (R = lambda/Delta lambda approximately 30 to 200), high sensitivity observations. Spectral purity and the reproducibility of the spectral shape are critical as well, when using the spectral shape to assign temperatures to dust grains or to gas clouds based on the wavelength and shape of molecular bands. These sensor attributes are also important to the use of wavelengths and ratios of solid state features to derive compositions of dust grains in celestial sources. The advent of high quality linear arrays of blocked impurity band (BIB) detectors of Si:As permitted the development of a state-of-the-art, patented, cooled prism spectrograph. Developed at The Aerospace Corporation largely with in-house funds, the Broadband Array Spectrograph System (BASS) has been used for a variety of remote sensing applications, but especially for IR astronomical studies on the Kuiper Airborne Observatory and at the NASA Infrared Telescope Facility (IRTF). The attributes of the spectrograph, specifically having the pupil imaged onto the 2 linear 58 element detector arrays so that the effects of guiding errors are minimized, being able to maximally exploit the limited observing time by acquiring all 116 spectral channels simultaneously, and having all spectral channels imaged through the same aperture so that spectral mapping is readily and reliably accomplished, afford the scientist with a unique opportunity to conduct both surveys of examples of many different types of sources as well as in-depth studies of a given class of object by thoroughly sampling the class members. This duality was demonstrated with the BASS through a combination of KAO flights where spectral maps were obtained as part of in-depth studies of specific source regions (such as Orion and W3) and flights where up to 21 legs/flight were used to survey many different sources of different types.

Russell, Ray W.↗
NASA NTRS1992

Compression of color-mapped images

In a standard image coding scenario, pixel-to-pixel correlation nearly always exists in the data, especially if the image is a natural scene. This correlation is what allows predictive coding schemes (e.g., DPCM) to perform efficient compression. In a color-mapped image, the values stored in the pixel array are no longer directly related to the pixel intensity. Two color indices which are numerically adjacent (close) may point to two very different colors. The correlation still exists, but only via the colormap. This fact can be exploited by sorting the color map to reintroduce the structure. The sorting of colormaps is studied and it is shown how the resulting structure can be used in both lossless and lossy compression of images.

Hadenfeldt, A. C.↗