Mitochondrial Fatty Acid Synthase Utilizes Multiple Acyl Carrier Protein Isoforms
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Avalanche photodiode detectors (APDs) at X-ray synchrotrons are typically limited to recording at most one photon per synchrotron pulse. Digitizing the APD amplifier outputs enables signal processing to accurately measure a mean count rate of at least four photons per pulse based on initial synchrotron measurements. Higher rates are readily achievable. This method allows APDs to be utilized for time-resolved measurements at much higher intensities than before.
Due to their potential applications in low-power consumption and/or multistate memory devices, multiferroic materials have attracted a lot of attention in the condensed matter community. As part of the effort to identify new multiferroic compounds, perovskite-based GdCrO 3 was studied in both bulk and thin film samples. A strong enhancement of the capacitance in a field suggested ferroelectric behaviour but significant leakage and no well developed P–E hysteresis loops were observed. Measurements clearly indicate the existence of a polar phase but only below 2 K (likely connected to Gd ordering). Here the determination of the magnetic structure through neutron diffraction collected on an isotopic 160 GdCrO 3 sample at the WISH diffractometer at ISIS is reported. The presence of three successive magnetic phases as a function of temperature (commensurate, spin re-orientation and incommensurate phases once the Gd order), previously only seen by magnetization, is confirmed. Using the most recent guidelines for reporting the determined structures, we highlight the benefits of using such nomenclature for discussing physical properties and consider possible mechanisms and couplings that led this seemingly rather isotropic system to display the complex structures observed.
Human mitochondrial manganese superoxide dismutase (MnSOD) converts superoxide into hydrogen peroxide and molecular oxygen, serving as a key defence against oxidative damage. Despite extensive studies, the full structural characterization of H 2 O 2 -binding sites in MnSOD remains largely unexplored. Previous H 2 O 2 -soaked MnSOD structures have identified two distinct H 2 O 2 -binding sites: one directly ligated to the catalytic manganese (LIG position) and another at the active-site gateway (PEO position) between the second-shell residues Tyr34 and His30. In this study, a kinetically impaired Gln143Asn MnSOD variant is used to trap and explore additional H 2 O 2 -binding sites beyond the second-shell solvent gate. In the wild-type enzyme, Gln143 mediates proton transfers with the manganese-bound solvent (WAT1) to drive redox cycling of the metal, which is necessary for effective superoxide dismutation. Substitution with Asn stalls catalysis because the increased distance from WAT1 disrupts critical proton-coupled electron-transfer (PCET) events, and the redox cycling of the active-site metal is impaired. This, in turn, stalls the electrostatic cycling of positive charge on the enzyme surface and enhances the likelihood of trapping transient H 2 O 2 -bound states in this variant. The results reveal several H 2 O 2 molecules leading up to the active site, in addition to the canonical LIG and PEO positions.
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Next generation solid state lighting enables unlimited control of light and serve much broader functions beyond the basic lighting for illuminance. The emerging lighting for productivity, well-being, healthcare, and growth have revolutionized the scope of lighting. To empower this unlimited controllability, a multi-channel tunable power-electronics driver is essential but has not been satisfactorily addressed so far. Existing solutions are lossy, large, and expensive, due to introduction of additional buck power conversion stages. This paper proposes a time-division multiplexing (TDM) LED driving system which eliminates those buck stages. The proposed design can directly pair with off-the-shelf pulse width modulated (PWM) controllers and since it inherits entire feature sets, it can achieve low loss at standby and at dimmed condition. The simple analog implementation avoids usage of expensive DSPs/MCU. GaN’s high switching frequency capability can push up the multiplexing frequency so a GaN-based flyback was built and proved the concept.
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Scientific workflows have become ubiquitous across scientific fields, and their execution methods and systems continue to be the subject of research and development. Most experimental evaluations of these workflows rely on workflow instances, which can be either real-world or synthetic, to ensure relevance to current application domains or explore hypothetical/future scenarios. The WfCommons project addresses this need by providing data and tools to support such evaluations. In this paper, we present an overview of WfCommons and describe two recent developments. Firstly, we introduce a workflow execution "tracer" for Nextflow, which significantly enhances the set of real-world instances available in WfCommons. Secondly, we describe a workflow instance "translator" that enables the execution of any real-world or synthetic WfCommons workflow instance using Dask. Our contributions aim to provide researchers and practitioners with more comprehensive resources for evaluating scientific workflows.
Before rotating, fossil fuel-based, synchronous generators (SGs) are phased out, in line with renewable generation goals, grid-forming (GFM) inverters are expected to parallel SGs. Primary droop control allows GFM inverters to share power without communication; however, it is necessary to dispatch GFM inverters and/or SGs with the desired output power for better energy management (e.g., one GFM inverter needs to charge the battery due to a low state of charge). Therefore, this paper develops an analytic approach to dispatching GFM inverters and SGs with the desired output power by shifting the droop intercept up/down while maintaining the same frequency operating point for improved transient stability. This concept is demonstrated through a pure hardware setup with two off-the-shelf inverters and one diesel generator under an islanded microgrid, and we provide insight on the real-world implementation of the proposed concept.
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