Engineering Papers⌕ Search

SEARCH · Engineering Papers

Results for “Location”

Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 1,063 records · Page 59
All sourcesNASA NTRSDOE OSTI
DOE OSTI2022

Biofilm formation and genomic features of Listeria monocytogenes strains isolated from meat and dairy industries located in Piedmont (Italy)

Listeria monocytogenes is considered a major challenge for the food industry as it can persist for long periods in food processing plants by forming biofilms. The aims of this study were: i) to assess the biofilm producing ability of 57 Listeria monocytogenes isolates previously subjected to whole-genome sequencing (WGS); ii) to compare the levels of biofilm formation with the presence or absence of biofilm associated genes. To determine the presence or absence of a known set of biofilm associated genes, a comparative genomic analysis was performed on each strain. Among Listeria monocytogenes isolates, 58 %, 38.5 % and 3.5 % exhibited weak, moderate or strong biofilm production, respectively. No difference in biofilm production was observed between food and environmental isolates. The percentage of Listeria monocytogenes strains isolated from meat products (57 %) classified as moderate or strong biofilm producers was higher than the percentage obtained for strains isolated from dairy products (28 %). Here, the presence of the Stress Survival Islet 1, the arsD stress gene and the truncated inlA protein was significantly associated with increased levels of biofilm. Combining biofilm phenotype with molecular and genotyping data may provide the opportunity to better understand the relationship between genes linked to biofilm formation in Listeria monocytogenes.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2021

Location and characterization of heterogeneous phases within Mary Rose wood

Preserving the Mary Rose oak hull for future generations is a major challenge due to the highly heterogeneous nature of waterlogged wooden artifacts, which contain polycrystalline, amorphous, and nanostructured materials that test traditional characterization methods. Effective conservation requires detailed knowledge of the distribution and chemical nature of these species to develop strategies for preventing multiple chemo-mechanical degradation pathways. Here, we apply synchrotron-based computed tomography total scattering methods to the Mary Rose keelson wood that provides valuable position-resolved structural information on multiple embedded species of different length and concentration scales. We identify 5 nm zinc sulfide nanoparticles in the wood, presumably deposits from bacteria operating on the sulfur energy cycle under the anaerobic conditions on the seabed. These are identified as precursors to acid attack on the wood upon removal to an aerobic environment. These insights inform not only next-generation conservation strategies, but also the efficacy and unforeseen issues of previous treatments.

75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND↗