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At least 1,063 records · Page 59
All sourcesNASA NTRSDOE OSTI
DOE OSTI2016

THE BURSTY STAR FORMATION HISTORIES OF LOW-MASS GALAXIES AT 0.4 < z < 1 REVEALED BY STAR FORMATION RATES MEASURED FROM H β AND FUV

We investigate the burstiness of star formation histories (SFHs) of galaxies at 0.4 < z < 1 by using the ratio of star formation rates (SFRs) measured from H β and FUV (1500 Å) (H β -to-FUV ratio). Our sample contains 164 galaxies down to stellar mass ( M {sub *}) of 10{sup 8.5} M {sub ⊙} in the CANDELS GOODS-N region, where Team Keck Redshift Survey Keck/DEIMOS spectroscopy and Hubble Space Telescope /WFC3 F275W images from CANDELS and Hubble Deep UV Legacy Survey are available. When the ratio of H β - and FUV-derived SFRs is measured, dust extinction correction is negligible (except for very dusty galaxies) with the Calzetti attenuation curve. The H β -to-FUV ratio of our sample increases with M {sub *} and SFR. The median ratio is ∼0.7 at M {sub *} ∼ 10{sup 8.5} M {sub ⊙} (or SFR ∼ 0.5 M {sub ⊙} yr{sup −1}) and increases to ∼1 at M {sub *} ∼ 10{sup 10} M {sub ⊙} (or SFR ∼ 10 M {sub ⊙} yr{sup −1}). At M {sub *} < 10{sup 9.5} M {sub ⊙}, our median H β -to-FUV ratio is lower than that of local galaxies at the same M {sub *}, implying a redshift evolution. Bursty SFH on a timescale of a few tens of megayears on galactic scales provides a plausible explanation for our results, and the importance of the burstiness increases as M {sub *} decreases. Due to sample selection effects, our H β -to-FUV ratio may be an upper limit of the true value of a complete sample, which strengthens our conclusions. Other models, e.g., non-universal initial mass function or stochastic star formation on star cluster scales, are unable to plausibly explain our results.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2023

Multi-site evaluation of stratified and balanced sampling of soil organic carbon stocks in agricultural fields

Estimating soil organic carbon (SOC) stocks in agricultural fields is essential for environmental and agronomic research, management, and policy. Stratified sampling is a classic strategy for estimating mean soil properties, and has recently been codified in SOC monitoring protocols. However, for the specific task of estimating the SOC stock of an agricultural field, concrete guidance is needed for which covariates to stratify on and how much stratification can improve estimation efficiency. It is also unknown how stratified sampling of SOC stocks compares to modern alternatives, notably doubly balanced sampling. To address these gaps, we collected high-density (average of 7 samples ha -1 ) and deep (average of 75 cm) measurements of SOC stocks at eight commercial fields under maize-soybean production in two US Midwestern states. We combined these measurements with a Bayesian geostatistical model to evaluate stratified and balanced sampling strategies that use a set of readily-available geographic, topographic, spectroscopic, and soil survey data. We examined the number of samples needed to achieve a given level of SOC stock estimation accuracy. While stratified sampling using these variables enables an average sample size reduction of 17% (95% CI, 11% to 23%) compared to simple random sampling, doubly balanced sampling is consistently more efficient, reducing sample sizes by 32% (95% CI, 25% to 37%). The data most important to these efficiency gains are a remotely-sensed SOC index, SSURGO estimates of SOC stocks, and the topographic wetness index. We conclude that in order to meet the urgent challenge of climate change, SOC stocks in agricultural fields could be more efficiently estimated by taking advantage of this readily-available data, especially with doubly balanced sampling.

54 ENVIRONMENTAL SCIENCES↗
NASA NTRS1988

Volcanic rocks and the geologic history of Mars

A number of questions exist regarding the geology of Mars which can be addressed by the proposed Mars rover-sample return mission. The use of a rover during the proposed mission greatly enhances the ability to investigate multiple aspects of Martian geology and geological history. Attempting to address all of the important questions may dilute the amount of information that can be obtained regarding each question and may result in no satisfactory answers. Prioritization is essential to a successful mission. The task of setting priorities is simplified somewhat when it is considered that answers to some of these questions do not require taking samples, and that for some questions, sample location is not as important as for others. The surface of Mars presents two distinct terrains, both of which have the potential to contain valuable information regarding the composition of Mars.

Salpas, P. A.↗
DOE OSTI2022

Investigating the limits of superconductivity in UTe 2

Abstract Spin-triplet bulk superconductors are a promising route to topological superconductivity, and UTe 2 is a recently discovered contender. The superconducting properties of UTe 2 , however, vary substantially as a function of the synthetic route, and even nonsuperconducting single crystals have been reported. To understand the driving mechanism suppressing superconductivity, we investigate UTe 2 single crystals grown close to the nonsuperconducting boundary (growth temperature ∼710 ∘ C) through a combination of thermodynamic and x-ray diffraction measurements. Specific heat measurements reveal a sharp decrease in the superconducting volume and a concomitant increase in the residual specific heat coefficient close to the nonsuperconducting boundary. Notably, these crystals are inhomogeneous and show an apparent double transition in specific heat measurements, similar to samples grown at much higher temperatures (∼1000 ∘ C). Our single crystal x-ray diffraction measurements reveal that there are two important tuning parameters: uranium vacancies and the atomic displacement along the c axis, which shows a twofold increase in samples with a reduced superconducting volume. Our results highlight the key role of local disorder along the uranium-uranium dimers and suggest that the apparent double superconducting transition is more likely to emerge close to the superconducting limits of UTe 2 .

Weiland, A. (ORCID:0000000171983559)↗
DOE OSTI2020

Speckle correlation as a monitor of X-ray free-electron laser induced crystal lattice deformation

X-ray free-electron lasers (X-FELs) present new opportunities to study ultrafast lattice dynamics in complex materials. While the unprecedented source brilliance enables high fidelity measurement of structural dynamics, it also raises experimental challenges related to the understanding and control of beam-induced irreversible structural changes in samples that can ultimately impact the interpretation of experimental results. This is also important for designing reliable high performance X-ray optical components. In this work, X-FEL beam-induced lattice alterations are investigated by measuring the shot-to-shot evolution of near-Bragg coherent scattering from a single crystalline germanium sample. It is shown that X-ray photon correlation analysis of sequential speckle patterns measurements can be used to monitor the nature and extent of lattice rearrangements. Additionally, abrupt, irreversible changes are observed following intermittent high-fluence monochromatic X-ray pulses, thus revealing the existence of a threshold response to X-FEL pulse intensity.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗
NASA NTRS2012

Mars Sample Return: The Next Step Required to Revolutionize Knowledge of Martian Geological and Climatological History

The capability of scientific instrumentation flown on planetary orbiters and landers has made great advances since the signature Viking mission of the seventies. At some point, however, the science return from orbital remote sensing, and even in situ measurements, becomes incremental, rather than revolutionary. This is primarily caused by the low spatial resolution of such measurements, even for landed instrumentation, the incomplete mineralogical record derived from such measurements, the inability to do the detailed textural, mineralogical and compositional characterization needed to demonstrate equilibrium or reaction paths, and the lack of chronological characterization. For the foreseeable future, flight instruments will suffer from this limitation. In order to make the next revolutionary breakthrough in understanding the early geological and climatological history of Mars, samples must be available for interrogation using the full panoply of laboratory-housed analytical instrumentation. Laboratory studies of samples allow for determination of parageneses of rocks through microscopic identification of mineral assemblages, evaluation of equilibrium through electron microbeam analyses of mineral compositions and structures, determination of formation temperatures through secondary ion or thermal ionization mass spectrometry (SIMS or TIMS) analyses of stable isotope compositions. Such details are poorly constrained by orbital data (e.g. phyllosilicate formation at Mawrth Vallis), and incompletely described by in situ measurements (e.g. genesis of Burns formation sediments at Meridiani Planum). Laboratory studies can determine formation, metamorphism and/or alteration ages of samples through SIMS or TIMS of radiogenic isotope systems; a capability well-beyond flight instrumentation. Ideally, sample return should be from a location first scouted by landers such that fairly mature hypotheses have been formulated that can be tested. However, samples from clastic sediments derived from an extensive region of Mars can provide important, detailed understanding of early martian geological and climatological history. Interrogating clastic "sediments" from the Earth, Moon and asteroids has allowed discovery of new crustal units, identification of now-vanished crust, and determination of the geological history of extensive, remote regions. Returned sample of martian fluvial and/or aeolian sediments, for example from Gale crater, could be "read like a book" in terrestrial laboratories to provide truly revolutionary new insights into early martian geological and climatological evolution.

Mittlefehldt, D. W.↗
DOE OSTI2019

Biomass Recalcitrance in Willow Under Two Biological Conversion Paradigms: Enzymatic Hydrolysis and Anaerobic Digestion

Biomass recalcitrance, the inherent resistance of plants towards deconstruction, negatively affects the viability of biorefineries. This trait is not only dictated by the properties of the biomass but also by the conversion system used and its interactions with specific features of the biomass. Here, biomass recalcitrance to anaerobic digestion (AD) was assessed using a biomethanation potential (BMP) assay. Plant material (n = 94) was selected from a large population of natural Salix viminalis accessions, previously evaluated for biomass recalcitrance using hydrothermal pretreatment-enzymatic hydrolysis. Correlations between yields from the two biological conversion systems were evaluated, as well as the influence of biomass compositional features, analyzed by pyrolysis-molecular beam mass spectrometry (py-MBMS), and other biomass physical properties on conversion performance. BMP values averaged 198.0 Nml CH 4 /g biomass after 94 days, ranging from 28.6 to 245.9. S lignin and carbohydrate-derived spectral features were positively correlated with performance under both systems, whereas G lignin, p-coumaric acid, and ferulic acid-derived ions were negatively correlated with yields and rates. Most spectral features were more strongly correlated with enzymatic hydrolysis yields compared to methane production. For early-stage methane production and rate, recalcitrance factors were similar compared to enzymatic hydrolysis, with weaker correlations observed at later timepoints. The results suggest that although variation in methane potential was considerably lower than enzymatic hydrolysis yields, a reduced recalcitrance under this system will still be of importance to improve early conversion rates. Spectral features of low methane-producing samples indicate the presence of inhibitory substances, warranting further study.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2018

A Comparison of the Diel Cycle of Modeled and Measured Latent Heat Flux During the Warm Season in a Colorado Subalpine Forest

Abstract Precipitation changes the physiological characteristics of an ecosystem. Because land‐surface models are often used to project changes in the hydrological cycle, modeling the effect of precipitation on the latent heat flux λE is an important aspect of land‐surface models. Here we contrast conditionally sampled diel composites of the eddy‐covariance fluxes from the Niwot Ridge Subalpine Forest AmeriFlux tower with the Community Land Model (CLM, version 4.5). With respect to measured λE during the warm season: for the day following above‐average precipitation, λE was enhanced at midday by ≈40 W m −2 (relative to dry conditions), and nocturnal λE increased from ≈10 W m −2 in dry conditions to over 20 W m −2 in wet conditions. With default settings, CLM4.5 did not successfully model these changes. By increasing the amount of time that rainwater was retained by the canopy/needles, CLM was able to match the observed midday increase in λE on a dry day following a wet day. Stable nighttime conditions were problematic for CLM4.5. Nocturnal CLM λE had only a small (≈3 W m −2 ) increase during wet conditions, CLM nocturnal friction velocity u ∗ was smaller than observed u ∗ , and CLM canopy air temperature was 2°C less than those measured at the site. Using observed u ∗ as input to CLM increased λE ; however, this caused CLM λE to be increased during both wet and dry periods. We suggest that sloped topography and the ever‐present drainage flow enhanced nocturnal u ∗ and λE . Such phenomena would not be properly captured by topographically blind land‐surface models, such as CLM.

Burns, Sean P.↗
DOE OSTI2025

Updating Critical Temperature Calculations for Several Secondary High Explosives

In designing experiments where high explosives (HEs) are heated, it is important to have an understanding of where thermal runaway may occur. This determination is often done by using the Frank-Kamenetskii (FK) equation. For several highly-studied HEs, the parameters necessary for FK calculations are typically referenced from decades-old literature, and more recent experimental data have shown that these values require adjustment. For example, some of the legacy parameters dangerously overpredict the critical temperature by tens of degrees relative to more recent observed values. Herein, we first summarize historical results and highlight the importance of insulation effects when estimating critical temperatures for small samples, which is especially relevant to small-scale aging, compatibility, and characterization experiments. Here, we then present updated FK parameters for several common secondary CHNO HEs like RDX, HMX, PETN, and TATB, as well as the first reported values for 2,6-Diamino-3,5-dinitropyrazine-1-oxide (LLM-105). Our updated parameters produce critical-temperature curves that are consistent with both mm-scale differentical scanning calorimetry (DSC) measurements and cm-scale data from the Lawrence Livermore National Laboratory one-dimensional time-to-explosion (ODTX) experiment. In analyzing the critical temperature versus sample size curves derived from the transcendental FK equation, we discovered that these curves are remarkably well described by a simple power-law function with a universal power of 0.142 and a HE-specific scaling factor.

45 MILITARY TECHNOLOGY, WEAPONRY, AND NATIONAL DEF↗
DOE OSTI2024

Two-loop mixed QCD-electroweak amplitudes for Z+jet production at the LHC: bosonic corrections

Abstract We present a calculation of the bosonic contribution to the two-loop mixed QCD-electroweak scattering amplitudes forZ-boson production in association with one hard jet at hadron colliders. We employ a method to calculate amplitudes in the ’t Hooft-Veltman scheme that reduces the amount of spurious non-physical information needed at intermediate stages of the computation, to keep the complexity of the calculation under control. We compute all the relevant Feynman integrals numerically using the Auxiliary Mass Flow method. We evaluate the two-loop scattering amplitudes on a two-dimensional grid in the rapidity and transverse momentum of theZboson, which has been designed to yield a reliable numerical sampling of the boosted-Zregion. This result provides an important building block for improving the theoretical modelling of a key background for monojet searches at the LHC.

Physics↗
DOE OSTI2019

Impact of reduced dimensionality on the correlation length and magnetization dynamics of the spin chain cobaltite Ca 3 Co 2 O 6

We have recently applied the magnetic field and temperature dependence of magnetic entropy change as a probe of the magnetic states of a single crystal of Ca 3 Co 2 O 6 to establish a more comprehensive magnetic phase diagram for this exotic system. The results are consistent with the spin-density wave description of the ground state and indicate the suppression of the modulated state in favor of a ferrimagnetic up-up-down arrangement of the spin chains with the application of a moderate field. We demonstrate, in this study, that the features of the static magnetic phase diagram are preserved when the grain size of Ca 3 Co 2 O 6 is reduced to 440 nm (below the maximum c-axis correlation length of ~550 nm in Ca 3 Co 2 O 6 ), although the polycrystalline materials show a non-saturating behavior, rounded steps, and reduced magnetization when compared to the single crystal. Our new finding of the multiple low-temperature magnetization steps in the 440 nm sample, together with conflicting reports concerning such steps in nanostructured samples, suggests that correlations in the ab plane are more important than correlations along the c-axis in the appearance of the plateaus. The relaxation processes in the system are studied through the decay of remanent magnetization, ac susceptibility, and Argand diagrams. The introduction of grain boundaries in Ca 3 Co 2 O 6 weakens the slow-dynamic state, widens the low-temperature distribution of relaxation times, and lowers activation energies within the Arrhenius-like relaxation regime.

36 MATERIALS SCIENCE↗
DOE OSTI2025

Microbiomes of frozen blood plasma samples reveal potential pathogens in wild birds and rodents

The lack of genomic data on pathogens from wildlife severely limits our ability to track transmission patterns and trace the origins of an outbreak. There are currently millions of wildlife samples in biobanks around the world, including blood samples. Blood has traditionally been viewed as a sterile environment in healthy individuals, but recent evidence suggests that this is not the case, especially for wild animals. Our goal was to determine whether frozen plasma samples can be surveyed using 16S sequencing to provide information about potential hosts for pathogens for a more complete understanding of disease systems. We sequenced blood plasma from wild North American deer mice ( Peromyscus maniculatus ) and American kestrels ( Falco sparverius ) that were cryogenically stored for 7 and 13 years, respectively, and compared two DNA extraction kits. The kestrel samples contained a very high number of reads that could not be identified to phylum compared to the mouse samples. The two kits differed in the phyla and genera that were detected, and the Zymo kit, which is optimized for plasma and serum, produced more high-quality reads for both kestrel and mouse samples. We identified several pathogenic genera, including Mycoplasma, Escherichia-Shigella , and Bartonella . Sequencing blood samples for pathogens could potentially have broad applications for identifying important reservoir hosts for pathogen transmission and provide a reduced set of species on which to follow up.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2019

Chemistry of Electrolyte Reduction on Lithium Silicide

Silicon anodes are promising for next-generation lithium-ion batteries due to high theoretical capacity. However, their performance and lifetime are currently limited by continuous electrolyte reduction and solid-electrolyte interphase (SEI) formation. Thus, SEI studies are important but often complicated due to the rough morphology of samples, buried interfaces, and the presence of binders. Here, we demonstrate the chemical origin of SEI formation by electrolyte reduction on lithium silicide thin films, synthesized by diffusion of pure evaporated lithium into smooth sputtered silicon. These model samples allowed for accurate estimation of irreversible capacity loss due to electrolyte reduction and for precise characterization of the resulting SEI by vibrational and photoelectron spectroscopies. Spectroscopic characterizations showed clear evidence that lithium silicide reduced electrolyte directly upon contact. Negligible first-cycle irreversible capacity loss was observed for the lithium silicide compared to silicon, indicating that the decomposition product of electrolyte on lithium silicide is able to stop further electrolyte reduction to a large extent. Fluoro-ethylene carbonate was shown to significantly affect the chemistry of electrolyte reduction on lithium silicide and subsequent cycling performance. The results of this basic study reveal the chemistry occurring at the interface of the lithium silicide and electrolyte and help in understanding the limited calendar lifetime of Li-ion batteries with Si anodes.

25 ENERGY STORAGE↗
DOE OSTI2025

Superheating gold beyond the predicted entropy catastrophe threshold

In their landmark study, Fecht and Johnson unveiled a phenomenon that they termed the ‘entropy catastrophe’, a critical point where the entropy of superheated crystals equates to that of their liquid counterparts. This point marks the uppermost stability boundary for solids at temperatures typically around three times their melting point. Despite the theoretical prediction of this ultimate stability threshold, its practical exploration has been prevented by numerous intermediate destabilizing events, colloquially known as a hierarchy of catastrophes, which occur at far lower temperatures. Here we experimentally test this limit under ultrafast heating conditions, directly tracking the lattice temperature by using high-resolution inelastic X-ray scattering. Our gold samples are heated to temperatures over 14 times their melting point while retaining their crystalline structure, far surpassing the predicted threshold and suggesting a substantially higher or potentially no limit for superheating. We point to the inability of our samples to expand on these very short timescales as an important difference from previous estimates. These observations provide insights into the dynamics of melting under extreme conditions.

Laser-produced plasmas↗
DOE OSTI2015

Comparative study of microfacies variation in two samples from the Chittenango member, Marcellus shale subgroup, western New York state, USA

This study demonstrates the significant lithologic variations that occur within the two shale samples from the Chittenango member of the Marcellus shale formation from western New York State in terms of mineralogical composition, type of lamination, pyrite occurrences and fossil content using thin section detailed description and field emission Scanning electron microscope (FESEM) with energy dispersive X-Ray Spectrum (EDX). This study is classified samples as laminated clayshale and fossiliferous carbonaceous shale. The most important detrital constituents of these shales are the clay mineral illite and chlorite, quartz, organic matter, carbonate mineral, and pyrite. The laminated clayshale has a lower amount of quartz and carbonate minerals than fossiliferous carbonaceous shale while it has a higher amount of clay minerals (chlorite and illite) and organic matter. FESEM analysis confirms the presence of chlorite and illite. The fossil content in the laminated clayshale is much lower than the fossiliferous carbonaceous shale. This can provide greater insights about variations in the depositional and environmental factors that influenced its deposition. This result can be compiled with the sufficient data to be helpful for designing the horizontal wells and placement of hydraulic fracturing in shale gas exploration and production.

58 GEOSCIENCES↗
DOE OSTI2019

Periodic Surface Morphology Characteristics of Tungsten Under Simultaneous Helium and Deuterium Bombardment at 30 keV

The ITER divertor will feature tungsten monoblocks as the plasma-facing component (PFC) that will be subject to extreme temperature and radiation environments. This paper reports the development of surface morphologies on tungsten under helium bombardment at high temperatures, which has important implications for safety, retention, and PFC erosion. Polycrystalline tungsten samples were implanted in the Dual Advanced Ion Simultaneous Implantation Experiment dual-beam ion implantation experiment at the University of Wisconsin-Madison with He-only and simultaneous He-D implantation at incidence angles of 55 deg, ion energies of 30 keV, and surface temperatures of 900°C to 1100°C. Morphologies resulting from angled incidence conditions differed from those produced under normal incidence bombardment at similar energy and temperature conditions in previous work. A variety of ordered and disordered morphologies dependent on grain orientation were observed for fluences up to 6 × 10 18 He cm –2 . These morphologies displayed dependencies on crystal orientation at low fluences and incident beam directions at higher fluences. Furthermore, these structures appeared, with variation, under both single-species He and mixed He-D implantations.

36 MATERIALS SCIENCE↗
DOE OSTI2015

SU-E-T-653: Quantifying Inter-Fraction Range Uncertainty for Input Into Robust Proton Planning

Purpose: To provide a method for quantifying centre- and tumour site-specific population range uncertainty due to inter-fraction motion for use within robust proton planning and plan analysis. PTV-based and probabilistic/robust plans depend upon accurate knowledge of geometric and range uncertainties. If the uncertainty model is inaccurate, both types of plan, PTV or probabilistic, could produce under-dosing of the target and/or overdosing of OAR. Methods: Daily mega-voltage volumetric imaging data from previously treated radiotherapy patients has been used to investigate inter-fraction changes to water equivalent path-length (WEPL). Daily image-guidance scans were carried out for each patient and corrected for changes in CTV position (using rigid transformations). An effective depth algorithm was used to determine residual range changes, after corrections were applied, by comparing WEPL within the CTV at each fraction for several beam angles. This method has been demonstrated for nine prostate and seven head and neck. Results: Inter-fraction range changes for the head and neck patient sample were Σ=3.39mm, σ=4.72mm and overall mean =−1.82mm; for prostate Σ=5.64mm, σ=5.91mm and overall mean =0.98 mm. The choice of beam angle for head and neck did not affect the inter-fraction range error significantly; however this was not the same for prostate. Greater range changes were seen using a lateral beam compared to an anterior beam for prostate due to relative motion of the prostate and femoral heads. Conclusion: A method has been developed to quantify population range changes due to inter-fraction motion. The results show a non-zero mean for these samples, as well as significant Σ and σ, highlighting the importance of quantifying uncertainties and use of image guidance. Such information can be used in robust optimisation algorithms and for analysing treatment plan robustness. It may also help in establishing beam start conditions at planning and for establishing adaptive planning protocols.

62 RADIOLOGY AND NUCLEAR MEDICINE↗
DOE OSTI2020

Measurement Error and Resolution in Quantitative Stable Isotope Probing: Implications for Experimental Design

Quantitative stable isotope probing (qSIP) estimates isotope tracer incorporation into DNA of individual microbes and can link microbial biodiversity and biogeochemistry in complex communities. As with any quantitative estimation technique, qSIP involves measurement error, and a fuller understanding of error, precision, and statistical power benefits qSIP experimental design and data interpretation. We used several qSIP data sets—from soil and seawater microbiomes—to evaluate how variance in isotope incorporation estimates depends on organism abundance and resolution of the density fractionation scheme. We assessed statistical power for replicated qSIP studies, plus sensitivity and specificity for unreplicated designs. As a taxon’s abundance increases, the variance of its weighted mean density declines. Nine fractions appear to be a reasonable trade-off between cost and precision for most qSIP applications. Increasing the number of density fractions beyond that reduces variance, although the magnitude of this benefit declines with additional fractions. Our analysis suggests that, if a taxon has an isotope enrichment of 10 atom% excess, there is a 60% chance that this will be detected as significantly different from zero (with alpha 0.1). With five replicates, isotope enrichment of 5 atom% could be detected with power (0.6) and alpha (0.1). Finally, we illustrate the importance of internal standards, which can help to calibrate per sample conversions of %GC to mean weighted density. These results should benefit researchers designing future SIP experiments and provide a useful reference for metagenomic SIP applications where both financial and computational limitations constrain experimental scope.

59 BASIC BIOLOGICAL SCIENCES↗