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At least 1,045 records · Page 58

Services provided in support of the planetary quarantine requirements of the National Aeronautics and Space Administration

The project to evaluate thermal sterilization for unmanned landers is reported. A temperature controlled oven with a nitrogen gas supply containing a known concentration of water is discussed. The studies show that bacillus lentus, bacillus brevis, bacillus coagulans, atypical bacillus spp., and actinomycete are isolated heat survivors. The thermal resistance is given for naturally occurring airborne bacterial spores collected on exposed teflon ribbons.

Favero, M. S.↗

Scientific and technical services directed toward the development of planetary quarantine measures for automated spacecraft

Planetary quarantine requirements and parameters are evaluated for their effects upon automated spacecraft flights data describing the heat resistance of naturally occuring microorganisms and sterilization requirements are analyzed and a possible method for assessment of these data is developed. Pertinent data from planetary mission microbial contamination logs are compiled and maintained in the quarantine document system.

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The mammalian response to lunar particulates.

The response of germfree mice to subcutaneous and intraperitoneal injection of aqueous suspensions of lunar fine material (LFM) was evaluated. Both uninjected mice and mice injected with dry heat sterilized LFM were included as controls. After injection, the majority of mice were subjected to serial sacrifice to assess the time course of the tissue response. A smaller group of animals were held for lifespan studies. The observations suggest that LFM is relatively insoluble in tissue and that, while acting as a low grade irritant, it has little tendency to evoke reactive fibrosis.

Holland, J. M.↗

Effect of simulated lunar impact on the survival of bacterial spores.

In order to test the effect of impact on organisms, the survival of bacterial spores after being propelled at high velocity in Pyrex and plastic beads into crushed basalt was measured. The beads were fired into sterilized canisters by both a conventional powder and a light gas gun. Results indicate that at the minimum (2.4 km/sec) lunar capture velocity, the number of colony forming units (CFUs) decreased by five orders of magnitude, and at 5.5 km/sec, statistically a more probable capture velocity, no CFUs were found. The decrease in CFUs observed with increasing velocity indicates that the spores were most probably killed by the impact.

Whitfield, O.↗

Heat-transfer thermal switch

Thermal switch maintains temperature of planetary lander, within definite range, by transferring heat. Switch produces relatively large stroke and force, uses minimum electrical power, is lightweight, is vapor pressure actuated, and withstands sterilization temperatures without damage.

Friedell, M. V.↗

Viking Lander antenna deployment mechanism

One of the mechanisms that is included in the configuration of the Viking Mars Lander is an antenna dish deployment mechanism which utilizes a mechanical escapement device for controlling deployment. The criteria require that the mechanism be capable of deploying the antenna under a wide range of aiding and retarding winds after landing on Mars but must limit the acceleration during deployment and latching of the antenna. Adding to the complexity of the design are the requirements for very accurate alignment after erection and heat sterilization prior to launch. This mechanism has been designed, fabricated and successfully tested to qualification test levels for usage on the Viking mission.

Hopper, K. H.↗

Dynamics of distribution and density of phreatophytes and other arid-land plant communities

The author has identified the following significant results. Six ERTS-1 images of the Tucson area, Arizona were analyzed to detect seasonal flushes of plant growth. Paired MSS-6 and MSS-5 bulk images were analyzed, using a ratioing technique, on the Electronic Satellite Image Analysis Console at Stanford Research Institute. Because of unique phenology, desert areas, covered only briefly by dense growths of ephemeral plants, are readily discerned. Grassland, evergreen forest, and riparian communities are also uniquely defined by their phenologies. Relatively sterile areas with little or no plant growth are easily discerned as are areas with varying degrees of plant productivity. The ratioing procedure detects plant coverage in excess of a threshold lying between 25% and 50%. The method is flexible and other coverage thresholds can be used.

Turner, R. M.↗

Explosives and pyrotechnic propellants for use in long term deep space missions

Explosives and pyrotechnic propellant materials which will withstand heat sterilization cycling at 125 C and ten year deep space aging under 10 to the minus 6th power torr and 66 C have been selected. The selection was accomplished through a detailed literature survey and an analytical evaluation of the physicochemical properties of the materials. The chemical components of the electroexplosive devices used in U.S. missiles and spacecraft were categorized into primary explosives, secondary explosives, and propellant ingredients. Kinetic data on such parameters as thermal decomposition and sublimation were obtained for these materials and used as a basis for the ten year life prediction. From these experimental data and some analytical calculations, a listing of candidate materials for deep space missions was made.

Gorzynski, C. S., Jr.↗

Evaluation of wet tantalum capacitors after exposure to extended periods of ripple current, volume 1

The application of tantalum capacitors in the Viking Lander includes both dc voltage and ripple current electrical stress, high temperature during nonoperating times (sterilization), and high vibration and shock loads. The capacitors must survive these severe environments without any degradation if reliable performance is to be achieved. A test program was established to evaluate both wet-slug tantalum and wet-foil capacitors under conditions accurately duplicating actual Viking applications. Test results of the electrical performance characteristics during extended periods of ripple current, the characteristics of the internal silver migration as a function for extended periods of ripple current, and the existence of any memory characteristics are presented.

Watson, G. W.↗

Environmental microbiology as related to planetary quarantine

The experimental design of a study to evaluate the effect of different cleaning methods and storage conditions on the dry heat resistance of Bacillus subtilis var. niger spores is described and the results for the first evaluation are reported. Specifically, the synergistic effect which occurs when spores are subjected simultaneously to dry heat and gamma radiation so as to be able to specify thermoradiation sterilization cycles was investigated. Attempts were made to understand the underlying mechanism(s) that lead to spore death from this combination of stresses. Data cover: (1) the survival of spores on surfaces at various temperatures in a precisely controlled environmental system, (2) the rate of destruction of these spores at ambient temperature when subjected to gamma radiation, and (3) the rate of destruction of spores when they are subjected to combined gamma radiation and thermal stresses.

Pflug, I. J.↗

Automated microbial metabolism laboratory

The design and rationale of an advanced labeled release experiment based on single addition of soil and multiple sequential additions of media into each of four test chambers are outlined. The feasibility for multiple addition tests was established and various details of the methodology were studied. The four chamber battery of tests include: (1) determination of the effect of various atmospheric gases and selection of that gas which produces an optimum response; (2) determination of the effect of incubation temperature and selection of the optimum temperature for performing Martian biochemical tests; (3) sterile soil is dosed with a battery of C-14 labeled substrates and subjected to experimental temperature range; and (4) determination of the possible inhibitory effects of water on Martian organisms is performed initially by dosing with 0.01 ml and 0.5 ml of medium, respectively. A series of specifically labeled substrates are then added to obtain patterns in metabolic 14CO2 (C-14)O2 evolution.

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The action of ultrasounds on Bezostaia 1 winter wheat grown in sand pots treated with Knop solution

The results of 3 years of research are presented concerning the increase in length and weight of the aerial biomass as compared with the underground biomass, as well as the productivity indexes of Bezostaia 1 winter wheat. The experiment involved two varieties, each of them with nine repetitions. Sterile sifted sand treated several times with a Knop solution during the vegetative period was used as a seed bed. The seeds were kept in water for 2 hours before treatment. Biometric and statistical measurements showed significant responses in the treated varieties.

Albu, N.↗

Cassette bacteria detection system

The design, fabrication, and testing of an automatic bacteria detection system, with a zero-g capability, based on the filter-capable approach, and intended for monitoring the sterility of regenerated water in spacecraft is discussed. The principle of detection is based on measuring the increase in chemiluminescence produced by the action of bacterial porphyrins on a luminol-hydrogen peroxide mixture. Viable organisms are detected by comparing the signal of an incubated water sample with an unincubated control. High signals for the incubated water sample indicate the presence of viable organisms.

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An observation about the relative hardiness of bacterial spores and planetary quarantine

Planetary quarantine objectives are shown to be critically dependent on the deviation in the actual decimal-reduction-time or D values (i.e., the time necessary to reduce the population to one-tenth of its original value) of organisms on spacecraft from the values chosen for spacecraft sterilization that have been selected conservatively relative to defined experimental procedures and bacterial spore stocks. New data indicate that these D values are not conservative when compared with those of naturally occurring organisms. The possible implications of these new data for planetary quarantine are analyzed.

Trauth, C. A., Jr.↗

Development of the Viking Mars lander thermal control subsystem design

Two Viking spacecraft, each consisting of a lander capsule coupled to an orbiter, will be launched toward Mars during the summer of 1975. About a year later, the orbiters will go into orbit around Mars and the landers will descend to the surface for 90-day landed missions. The lander must withstand a wide variety of environmental and operational conditions during all phases of the mission, including prelaunch sterilization. On the surface of Mars, the lander internal temperatures must be controlled under widely varying thermal environments and atmospheric conditions. The lander thermal design is based on the maximum use of passive techniques and is integrated into the overall vehicle design and operation. The solutions to the unusual combinations of design problems and a summary of the results of full scale model testing under simulated mission conditions are presented.

Morey, T. F.↗

Integrated water and waste management system for future spacecraft

Over 200 days of continuous testing have been completed on an integrated waste management-water recovery system developed by General Electric under a jointly funded AEC/NASA/AF Contract. The 4 man system provides urine, feces, and trash collection; water reclamation; storage, heating and dispensing of the water; storage and disposal of the feces and urine residue and all of other nonmetallic waste material by incineration. The heat required for the 1200 deg F purification processes is provided by a single 420-w radioisotope heater. A second 836-w radioisotope heater supplemented by 720 w of electrical heat provides for distillation and water heating. Significant test results are no pre-or-post treatment, greater than 98 per cent potable water recovery, approximately 95 per cent reduction in solids weight and volume, all outflows are sterile with the water having no bacteria or virus, and the radioisotope capsule radiation level is only 7.9 mrem/hr unshielded at 1 m (neutrons and gamma).

Ingelfinger, A. L.↗

Silver ion bactericide system

Description of a preliminary flight prototype system which uses silver ions as the bactericide to preserve sterility of the water used for human consumption and hygiene in the Space Shuttle Orbiter. The performance of silver halide columns for passively dosing fuel cell water with silver ions is evaluated. Tests under simulated Orbiter mission conditions show that silver ion doses of 0.05 ppm are bactericidal for Pseudomonas aeruginosa and Type IIIa, the two bacteria found in Apollo potable water systems. The design of the Advance Prototype Silver Ion Water Bactericide System now under development is discussed.

Jasionowski, W. J.↗