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At least 1,045 records · Page 58

Comparative transduction mechanisms of hair cells in the bullfrog utriculus. II. Sensitivity and response dynamics to hair bundle displacement

1. Hair cells in whole-mount in vitro preparations of the utricular macula of the bullfrog (Rana catesbeiana) were selected according to their macular location and hair bundle morphology. The sensitivity and response dynamics of selected hair cells to natural stimulation were examined by recording their voltage responses to step and sinusoidal hair bundle displacements applied to their longest stereocilia. 2. The voltage responses of 31 hair cells to sinusoidal hair bundle displacements were characterized by their gains and phases, taken with respect to peak hair bundle displacement. The gains of Type B and Type C cells at both 0.5 and 5.0 Hz were markedly lower than those of Type F and Type E cells. Phases, with the exception of Type C cells, lagged hair bundle displacement at 0.5 Hz. Type C cells had phase leads of 25-40 degrees. At 5.0 Hz, response phases in all cells were phase lagged with respect to those at 0.5 Hz. Type C cells had larger gains and smaller phase leads at 5.0 Hz than at 0.5 Hz, suggesting the presence of low-frequency adaptation. 3. Displacement-response curves, derived from the voltage responses to 5.0-Hz sinusoids, were sigmoidal in shape and asymmetrical, with the depolarizing response having a greater magnitude and saturating less abruptly than the hyperpolarizing response. When normalized to their largest displacement the linear ranges of these curves varied from < 0.5 to 1.25 microns and were largest in Type B and smallest in Type F and Type E cells. Sensitivity, defined as the slope of the normalized displacement-response curve, was inversely correlated with linear range. 4. The contribution of geometric factors associated with the hair bundle to linear range and sensitivity were predicted from realistic models of utricular hair bundles created using morphological data obtained from light and electron microscopy. Three factors, including 1) the inverse ratio of the lengths of the kinocilium and longest stereocilia, representing the lever arm between kinociliary and stereociliary displacement; 2) tip link extension/linear displacement, largely a function of stereociliary height and separation; and 3) stereociliary number, an estimate of the number of transduction channels, were considered in this analysis. The first of these factors was quantitatively more important than the latter two factors and their total contribution was largest in Type B and Type C cells. Theoretical models were also used to calculate the relation between rotary and linear displacement.(ABSTRACT TRUNCATED AT 400 WORDS).

Non-NASA Center↗

Important influence of respiration on human R-R interval power spectra is largely ignored

Frequency-domain analyses of R-R intervals are used widely to estimate levels of autonomic neural traffic to the human heart. Because respiration modulates autonomic activity, we determined for nine healthy subjects the influence of breathing frequency and tidal volume on R-R interval power spectra (fast-Fourier transform method). We also surveyed published literature to determine current practices in this burgeoning field of scientific inquiry. Supine subjects breathed at rates of 6, 7.5, 10, 15, 17.1, 20, and 24 breaths/min and with nominal tidal volumes of 1,000 and 1,500 ml. R-R interval power at respiratory and low (0.06-0.14 Hz) frequencies declined significantly as breathing frequency increased. R-R interval power at respiratory frequencies was significantly greater at a tidal volume of 1,500 than 1,000 ml. Neither breathing frequency nor tidal volume influenced average R-R intervals significantly. Our review of studies reporting human R-R interval power spectra showed that 51% of the studies controlled respiratory rate, 11% controlled tidal volume, and 11% controlled both respiratory rate and tidal volume. The major implications of our analyses are that breathing parameters strongly influence low-frequency as well as respiratory frequency R-R interval power spectra and that this influence is largely ignored in published research.

NASA Discipline Cardiopulmonary↗

Hemodynamic and autonomic nervous system responses to mixed meal ingestion in healthy young and old subjects and dysautonomic patients with postprandial hypotension

BACKGROUND. Although postprandial hypotension is a common cause of falls and syncope in elderly persons and in patients with autonomic insufficiency, the pathophysiology of this disorder remains unknown. METHODS AND RESULTS. We examined the hemodynamic, splanchnic blood pool, plasma norepinephrine (NE), and heart rate (HR) power spectra responses to a standardized 400-kcal mixed meal in 11 healthy young (age, 26 +/- 5 years) and nine healthy elderly (age, 80 +/- 5 years) subjects and 10 dysautonomic patients with symptomatic postprandial hypotension (age, 65 +/- 16 years). Cardiac and splanchnic blood pools were determined noninvasively by radionuclide scans, and forearm vascular resistance was determined using venous occlusion plethysmography. In healthy young and old subjects, splanchnic blood volume increased, but supine blood pressure remained unchanged after the meal. In both groups, HR increased and systemic vascular resistance remained stable. Forearm vascular resistance and cardiac index increased after the meal in elderly subjects, whereas these responses were highly variable and of smaller magnitude in the young. Young subjects demonstrated postprandial increases in low-frequency HR spectral power, representing cardiac sympatho-excitation, but plasma NE remained unchanged. In elderly subjects, plasma NE increased after the meal but without changes in the HR power spectrum. Patients with dysautonomia had a large postprandial decline in blood pressure associated with no change in forearm vascular resistance, a fall in systemic vascular resistance, and reduction in left ventricular end diastolic volume index. HR increased in these patients but without changes in plasma NE or the HR power spectrum. CONCLUSIONS. 1) In healthy elderly subjects, the maintenance of blood pressure homeostasis after food ingestion is associated with an increase in HR, forearm vascular resistance, cardiac index, and plasma NE. In both young and old, systemic vascular resistance is maintained. 2) Dysautonomic patients with postprandial hypotension fail to maintain systemic vascular resistance after a meal. This impairment in vascular response to meal ingestion may underlie the development of postprandial hypotension. 3) The measurement of mean HR or plasma NE does not adequately characterize autonomic cardiac control. Power spectral analysis suggests an impairment in the postprandial autonomic modulation of HR in healthy elderly and dysautonomic subjects, possibly predisposing to hypotension when vascular compensation is inadequate.

Non-NASA Center↗

In vivo assessment of forearm bone mass and ulnar bending stiffness in healthy men

The cross-sectional bending stiffness EI of the ulna was measured in vivo by mechanical resistance tissue analysis (MRTA) in 90 men aged 19-89 years. MRTA measures the impedance response of low-frequency vibrations to determine EI, which is a reflection of elastic modulus E and moment of inertia I for the whole ulna. EI was compared to conventional estimates of bone mineral content (BMC), bone width (BW), and BMC/BW, which were all measured by single-photon absorptiometry. Results obtained from the nondominant ulna indicate that BW increases (r = 0.27, p = 0.01) and ulnar BMC/BW decreases (r = -0.31, p < or = 0.005) with age. Neither BMC nor EI declined with age. The single best predictor of EI was BW (r2 = 0.47, p = 0.0001), and further small but significant contributions were made by BMC (r2 = 0.53, p = 0.0001) and grip strength (r2 = 0.55, p = 0.0001). These results suggest that the resistance of older men to forearm fracture is related to age-associated changes in the moment of inertia achieved by redistributing bone mineral farther from the bending axis. We conclude that the in vivo assessment of bone geometry offers important insights to the comprehensive evaluation of bone strength.

NASA Center ARC↗

A new technique for simultaneous monitoring of electrocardiogram and walking cadence

A new technique for simultaneously recording continuous electrocardiographic (ECG) data and walking step rate (cadence) is described. The ECG and gait signals are recorded on 2 channels of an ambulatory Holter monitor. Footfall is detected using ultrathin, force-sensitive foot switches and is frequency modulated. The footfall signal provides an indication of the subject's activity (walking or standing), as well as the instantaneous walking rate. Twenty-three young and elderly subjects were studied to demonstrate the use of this ECG and gait recorder. High-quality gait signals were obtained in all subjects, and the effects of walking on the electrocardiogram were assessed. Initial investigation revealed the following findings: (1) Although walking rates were similar in young and elderly subjects, the elderly had both decreased heart rate (HR) variability (p < 0.005) and increased cadence variability (p < 0.0001). (2) Overall, there was an inverse relation between HR and cadence variability (r = -0.73). Three elderly subjects with no known cardiac disease had HR and cadence variability similar to those of the young, whereas elderly subjects with history of congestive heart failure were among those with the lowest HR variability and the highest cadence variability. (3) Low-frequency (approximately equal to 0.1 Hz) HR oscillations (frequently observed during standing) persisted during walking in all young subjects. (4) In some subjects, both step rate and HR oscillated at the same low frequency (approximately equal to 0.1 Hz) previously identified with autonomic control of the baroreflex.(ABSTRACT TRUNCATED AT 250 WORDS).

NASA Discipline Cardiopulmonary↗

Electrophysiology of the mammillary complex in vitro. II. Medial mammillary neurons

1. The electrophysiological properties of guinea pig medial mammillary body (MMB) neurons were studied using an in vitro slice preparation. 2. The neurons (n = 80) had an average resting potential of -57 +/- 5.5 (SD) mV, an input resistance of 176 +/- 83 M omega, and a spike amplitude of 58 +/- 15.7 mV. Most of the neurons were silent at rest (n = 52), but some fired spontaneous single spikes (n = 16) or spike bursts (n = 14). 3. The main electrophysiological characteristic of MMB neurons was the ability to generate Ca(2+)-dependent regenerative events, which resulted in very robust burst responses. However, this regenerative event was not the same for all neurons, ranging from typical low-threshold Ca2+ spikes (LTSs) to intermediate-threshold plateau potentials (ITPs). 4. The ITPs were distinct from the LTSs in that they lasted > or = 100 ms and were not inactivated at membrane potentials at or positive to -55 mV. 5. Some cells with a prominent ITP and no LTS (n = 36) displayed repetitive, usually rhythmic, bursting (n = 14). This ITP could be powerful enough to maintain rhythmic membrane potential oscillations after pharmacological block of Na+ conductances. 6. A group of 32 MMB neurons displayed complex bursting that was generated by activation of both LTSs and ITPs. This was established on the basis of their distinct time- and voltage-dependent characteristics. In a group of neurons (n = 14), the burst responses were exclusively generated by an LTS; however, a Ca(2+)-dependent plateau potential contributed to the generation of rebound-triggered oscillatory firing. 7. In addition to the Ca(2+)-dependent LTS and/or ITP, MMB neurons always displayed high-threshold Ca2+ spikes after reduction of K+ conductances with tetraethylammonium. 8. MMB neurons display one of the richer varieties of voltage-dependent Ca2+ conductances so far encountered in mammalian CNS. We propose that the very prominent endogenous bursting and oscillatory properties of MB neurons allow this nuclear complex to function as an oscillatory relay for the transmission of low-frequency rhythmic activities throughout the limbic circuit.

NASA Discipline Neuroscience↗

Real-Time Data from the Orbital Acceleration Research Experiment (OARE)

The objective of the Orbital Acceleration Research Experiment (OARE) is to measure, with high accuracy, the low-frequency, low-magnitude acceleration levels onboard the space shuttle. The shuttle experiences acceleration from atmospheric drag, gravity gradient forces, shuttle rotations, crew activities, water/waste dumps, and shuttle attitude thrusters. The OARE instrument has successfully flown on five past shuttle missions and is scheduled for five upcoming microgravity science missions. The data collected by OARE will be utilized by microgravity scientists to better predict and analyze the influence and effects of the shuttle's on-orbit microgravity environment on experiments in materials, combustion, and fluids research.

Source record↗

The Galileo/Mars Observer/Ulysses Coincidence Experiment

From March 21 to April 11, 1993 the Galileo, Mars Observer and Ulysses spacecraft were tracked in a coincidence experiment, searching for low-frequency (~millihertz) gravitational radiation. In the spacecraft Doppler technique, the earth and a distant spacecraft act as separated test masses.

Galileo Ulyses Doppler Technique gravitational wav↗

Alfvenic Solitons in Ultrarelativistic Electron-Position Plasmas

In electron-positron plasmas some of the plasma modes are decoupled due to the equal charge-to-mass ratio of both species. We derive the dispersion law for a low-frequency, generalized X-mode, which exists at all angles of propagation with respect to the static magnetic field.

Electron-positron plasmas Pulsars Nonlinear Modes↗

The Galileo/Mars Observer/Ulysses Coincidence Experiment

From March 21 to April 11, 1993 the Galileo, Mars Observer and Ulysses spacecraft were tracked in a coincidence experiment, searching for low-frequency (milihertz gravitational radiation.

Doppler Tracking Galileo Mars Observer Ulysses↗

Gravitational Wave Search with the Clock Mission (abstract)

Doppler tracking of distant spacecraft is the only method currently available for search for gravitational waves in the low-frequency band. Experiments to date and those planned for the near future all involve.

clocks gravitational waves doppler frequency stand↗

Thermal Stability Analysis for a Heliocentric Gravitational Radiation Detection Mission

The Laser Interferometer Space Antenna (LISA) mission is designed for detailed studies of low-frequency gravitational radiation. The mission is currently a candidate for ESA's post-Horizon 2000 program. Thermal noise affects the measurement in at least two ways. Thermal variation of the length of the optical cavity to which the lasers are stabilized introduces phase variations in the interferometer signal, which have to be corrected for by using data from the two arms separately.

Laser Interferometer Space Antenna LISA gravitatio↗

(abstract) Spacecraft Doppler Tracking with the Deep Space Network in the Search for Gravitational Waves

The Deep Space Network generates accurate radio science data observables for investigators who use radio links between spacecraft and the Earth to examine small changes in the phase and/or amplitude of the signal to study a wide variety of structures and phenomena in space. Several such studies are directed at aspects of the theory of general relativity such as gravitational redshift and gravitational waves. A gravitational wave is a propagating, polarized gravitational field, a ripple in the curvature of space-time. In Einstein's theory of general relativity, the waves are propagating solutions of the Einstein field equations. Their amplitudes are dimensionless strain amplitudes that change the fractional difference in distance between test masses and the rates at which separated clocks keep time. Predicted by all relativistic theories of gravity, they are extremely weak (the ratio of gravitational forces to electrical forces is about 10(sup -40)) and are generated at detectable levels only by astrophysical sources - very massive sources under violent dynamical conditions. The waves have never been detected but searches in the low-frequency band using Doppler tracking of many spacecraft have been conducted and others are being planned. Upper limits have been placed on the gravitational wave strength with the best sensitivities to date are for periodic waves being 7 x 10(sup -15).

gravitational waves theory of general relativity D↗

Interplanetary Scintillation

Interplanetary scintillation (IPS) has been used as a diagnostic of solar wind speed and interplanetary plasma turbulence, allowing inference of speed and electron density power spectrum close to the Sun and out of the ecliptic. In that context, IPS is 'signal' and provides scientifically interesting data. IPS is also of interest because amplitude and phase perturbations imposed on radio waves are 'noise' for telemetry and precision Doppler tracking of deep space probes and for some radio astronomical observations. This paper briefly reviews the connection between scattering observables and the electron density power spectrum. Interplanetary phase scintillation on time scales of 100 to 10 000 seconds is an important noise in mass determinations of small solar system bodies during space-probe fly-bys and in searches for low-frequency gravitational radiation.

waves deep space probes electron density power spe↗

(abstract) System Performance of the Joint Galileo/Mars Observer/Ulysses 1993 Gravitational Wave Experiment

From March 21 to April 11, 1993, the Galileo, Mars Observer, and Ulysses spacecraft were tracked almost continuously in a coincidence experiment to search for low-frequency (~millihertz) gravitational radiation. We report here a first statistical assessment of the noise characteristics of the data, with particular attention to the performance of the radio science instrumentation itself.

gravitational waves radio science noise Doppler ef↗

Airflow sensing system

Disclosed is an airflow sensing system for determining the type of airflow flowing over a flight surface. A hot film sensor is driven by a constant voltage feedback circuit that maintains the voltage across the sensor at a predetermined level. A signal processing circuit receives an output signal of the feedback circuit and determines whether the output signal is indicative of laminar, transitional or turbulent airflow. Transitional airflow is distinguished from turbulent airflow by a signal having significant energy in a low-frequency passband from 50-80 Hz. The signal processing circuit drives a three-color LED display to provide a visual indication of the type of airflow being sensed.

Gelbach, Herman R.↗

Parametric Flutter Analysis of the TCA Configuration and Recommendation for FFM Design and Scaling

The current HSR Aeroelasticity plan to design, build, and test a full span, free flying transonic flutter model in the TDT has many technical obstacles that must be overcome for a successful program. One technical obstacle is the determination of a suitable configuration and point in the sky to use in setting the scaling point for the ASE models program. Determining this configuration and point in the sky requires balancing several conflicting requirements, including model buildability, tunnel test safety, and the ability of the model to represent the flutter mechanisms of interest. As will be discussed in detail in subsequent sections, the current TCA design exhibits several flutter mechanisms of interest. It has been decided that the ASE models program will focus on the low frequency symmetric flutter mechanism, and will make no attempt to investigate high frequency flutter mechanisms. There are several reasons for this choice. First, it is believed that the high frequency flutter mechanisms are similar in nature to classical wing bending/torsion flutter, and therefore there is more confidence that this mechanism can be predicted using current techniques. The low frequency mode, on the other hand, is a highly coupled mechanism involving wing, body, tail, and engine motion which may be very difficult to predict. Second, the high frequency flutter modes result in very small weight penalties (several hundred pounds), while suppression of the low frequency mechanism inside the flight envelope causes thousands of pounds to be added to the structure. In order to successfully test the low frequency flutter mode of interest, a suitable starting configuration and point in the sky must be identified. The configuration and point in the sky must result in a wind tunnel model that (1) represents the low-frequency wing/body/engine/empennage flutter mechanisms that are unique to HSCT configurations, (2) flutters at an acceptably low frequency in the tunnel, (3) flutters at an acceptably low dynamic pressure in the tunnel, (4) allows sufficient weight for model buildability without inordinately high cost, and (5) has significant separation between the target flutter mechanism and other, potentially catastrophic, flutter mechanisms.

Baker, Myles↗

Gravitational Wave Search With the Clock Mission

Doppler tracking of distant spacecraft is the only method currently available to search for gravitational waves in the low-frequency (~0.0001-0.1 Hz) band. Suppose that the Clock Mission spacecraft is transferred into a suitable interplanetary orbit, I discuss here how the on-board frequency standard could be employeed with an all-Ka-band radio system using the very high stability Deep Space Network station DSS 25 being instrumented for Cassini. With this configuration, the Clock Mission could search for gravitational waves at a sensitivity limited by the frequency standards, rather than plasma or tropospheric scintillation effects, whenever the sun-earth-spacecraft angle is greater than 90 degrees.

Clock↗