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DOE OSTI2023

Disturbed, diffuse, or just missing? A global study of the H I content of Hickson compact groups

Hickson compact groups (HCGs) are dense configurations of four to ten galaxies, whose H I morphology appears to follow an evolutionary sequence of three phases, with gas initially confined to galaxies, then significant amounts spread throughout the intra-group medium, and finally with almost no gas remaining in the galaxies themselves. It has also been suggested that several groups may harbour a diffuse H I component that is resolved out by interferometric observations. The H I deficiency of HCGs is expected to increase as the H I morphological phase progresses along the evolutionary sequence. If this is the case, H I deficiency would be a rough proxy for the age and evolutionary state of a HCG. We aim to test this hypothesis for the first time using a large sample of HCGs and to investigate the evidence for diffuse H I in HCGs. We performed a uniform reduction of all publicly available VLA H I observations (38 HCGs) with a purpose-built pipeline that also maximises the reproducibility of this study. The resulting H I data cubes were then analysed with the latest software tools to perform a manual separation of emission features into those belonging to galaxies and those extending into the intra-group medium. We thereby classified the H I morphological phase of each group as well as quantified their H I deficiency compared to galaxies in isolation. We find little evidence that H I deficiency can be used as a proxy for the evolutionary phase of a compact group in either of the first two phases, with the distribution of H I deficiency being consistent in both. However, for the final phase, the distribution clearly shifts to high H I deficiencies, with more than 90% of the expected H I content typically missing. Across all HCGs studied, we identify a few cases where there is strong evidence for a diffuse gas component in the intra-group medium, which might be detectable with improved observations. We also classify a new sub-phase where groups contain a lone H I -bearing galaxy, but are otherwise devoid of gas. The new morphological phase we have identified is likely the result of an evolved, gas-poor group acquiring a new, gas-rich member. The large spread of H I deficiencies in the first two morphological phases suggests that there is a broad range of initial H I content in HCGs, which is perhaps influenced by large-scale environment, and that the timescale for morphological changes is, in general, considerably shorter than the timescale for the destruction or consumption of neutral gas in these systems.

79 ASTRONOMY AND ASTROPHYSICS↗

NASA NTRS1971

High-power microstrip RF switch

A microstrip-type single-pole double-throw (SPDT) switch whose RF and bias portions contain only a metallized alumina substrate and two PIN diodes has been developed. A technique developed to eliminate the dc blocking capacitors needed for biasing the diodes is described. These capacitors are extra components and could lower the reliability significantly. An SPDT switch fabricated on a 5.08 x 5.08 x 0.127-cm (2 x 2 x 0.050-in.) substrate has demonstrated an RF power-handling capability greater than 50 W at S-band. The insertion loss is less than 0.25 db and the input-to-off port isolation is greater than 36 db over a bandwidth larger than 30 MHz. The input voltage standing-wave ratio is lower than 1.07 over the same bandwidth. Theoretical development of the switch characteristics and experimental results, which are in good agreement with theory, are presented.

Choi, S. D.↗

NASA NTRS1993

Boundary-layer receptivity due to distributed surface imperfections of a deterministic or random nature

Acoustic receptivity of a Blasius boundary layer in the presence of distributed, 2D surface irregularities is investigated analytically. It is shown that, out of the entire spatial spectrum of the surface irregularities, only a narrow band of Fourier components can lead to an efficient conversion of the acoustic input at any given frequency to an unstable eigenmode of the boundary-layer flow. Random irregularities which are spatially homogeneous in nature, and also possess a continuous spectrum in the streamwise direction, lead to instability amplitudes that are intermediate to those caused by the periodic and isolated irregularities, respectively, being, in fact, of the same order as the geometrical mean of the amplitudes in the latter two cases. A physical explanation for these asymptotic scalings is given, in addition to providing an analytical expression for the expected value of the instability amplitude for an ensemble of statistically irregular surfaces with random phase distributions.

Choudhari, Meelan↗

NASA NTRS1995

An Integration of the Turbojet and Single-Throat Ramjet

A turbine-engine-based hybrid propulsion system is described. Turbojet engines are integrated with a single-throat ramjet so as to minimize variable geometry and eliminate redundant propulsion components. The result is a simple, lightweight system that is operable from takeoff to high Mach numbers. Non-afterburning turbojets are mounted within the ramjet duct. They exhaust through a converging-diverging (C-D) nozzle into a common ramjet burner section. At low speed the ejector effect of the C-D nozzle aerodynamically isolates the relatively high pressure turbojet exhaust stream from the ramjet duct. As the Mach number increases, and the turbojet pressure ratio diminishes, the system is biased naturally toward ramjet operation. The common ramjet burner is fueled with hydrogen and thermally choked, thus avoiding the weight and complexity of a variable geometry, split-flow exhaust system. The mixed-compression supersonic inlet and subsonic diffuser are also common to both the turbojet and ramjet cycles. As the compressor face total temperature limit is approached, a two-position flap within the inlet is actuated, which closes off the turbojet inlet and provides increased internal contraction for ramjet operation. Similar actuation of the turbojet C-D nozzle flap completes the enclosure of the turbojet. Performance of the hybrid system is compared herein to that of the discrete turbojet and ramjet engines from takeoff to Mach 6. The specific impulse of the hybrid system falls below that of the non-integrated turbojet and ramjet because of ejector and Rayleigh losses. Unlike the discrete turbojet or ramjet however, the hybrid system produces thrust over the entire Mach number range. An alternate mode of operation for takeoff and low speed is also described. In this mode the C-D nozzle flap is deflected to a third position, which closes off the ramjet duct and eliminates the ejector total pressure loss.

Trefny, C. J.↗

NASA NTRS1996

Tilt Rotor Aircraft Aeroacoustics

A fleet of civil tilt rotor transports offers a means of reducing airport congestion and point-to-point travel time. The speed, range, and fuel economy of these aircraft, along with their efficient use of vertiport area, make them good candidates for short-to-medium range civil transport. However, to be successfully integrated into the civilian community, the tilt rotor must be perceived as a quiet, safe, and economical mode of transportation that does not harm the environment. In particular, noise impact has been identified as a possible barrier to the civil tilt rotor. Along with rotor conversion-mode flight, and blade-vortex interaction noise during descent, hover mode is a noise problem for tilt rotor operations. In the present research, tilt rotor hover aeroacoustics have been studied analytically, experimentally, and computationally. Various papers on the subject were published as noted in the list of publications. More recently, experimental measurements were made on a 1/12.5 scale model of the XV-15 in hover and analyses of this data and extrapolations to full scale were also carried out. A dimensional analysis showed that the model was a good aeroacoustic approximation to the full-scale aircraft, and scale factors were derived to extrapolate the model measurements to the full-scale XV-15. The experimental measurements included helium bubble flow visualization, silk tuft flow visualization, 2-component hot wire anemometry, 7-hole pressure probe measurements, vorticity measurements, and outdoor far field acoustic measurements. The hot wire measurements were used to estimate the turbulence statistics of the flow field into the rotors, such as length scales, velocity scales, dissipation, and turbulence intermittency. Several different configurations of the model were tested: (1) standard configurations (single isolated rotor, two rotors without the aircraft, standard tilt rotor configuration); (2) flow control devices (the 'plate', the 'diagonal fences'); (3) basic configuration changes (increasing the rotor/rotor spacing, reducing the rotor plane/wing clearance. operating the rotors out of phase). Also, an approximation to Sikorsky's Variable Diameter Tilt Rotor (VDTR) configuration was tested, and some flow measurements were made on a semi-span configuration of the model. Acoustic predictions were made using LOWSON.M, a Mathematica code Mean aerodynamic models were developed based on hover performance predictions from HOVER.FOR. This hover prediction code used blade element theory for the aerodynamics, and Prandtl's Vortex theory to model the wake, along with empirical formulas for the effects of Reynolds number, Mach number, and stall. Aerodynamic models were developed from 7-hole pressure probe measurements of the mean velocity into the model rotors.

George, Albert R.↗

NASA NTRS2005

Atmospheric Electron-induced X-Ray Spectrometer (AEXS) Instrument Development

Introduction: This paper describes the progress in data acquisition and establishing the observational capability of the AEXS instrument. The AEXS is a miniature instrument[1-4] based on the excitation of characteristic X-Ray Fluorescence (XRF) and luminescence spectra using a focused electron beam which enables nondestructive evaluation of sample surfaces in planetary ambient atmospheres. In situ operation is obtained through the use of a thin electron transmissive membrane to isolate the vacuum of the AEXS source from the outside ambient atmosphere. Thus eliminating the need for a vacuum pumped sample chamber as is common in all laboratory SEM s. The transmitted electrons impinge on the sample exciting XRF spectra from the irradiated spot on in-situ or collected samples with sub-mm to cm-scale spatial resolution at Mars atmospheric pressure. The AEXS system (Fig 1) consists of a high-energy (>10keV) electron gun encapsulated by the isolation membrane, an XRF detection and analyzer system, and a high voltage power supply. The XRF data are analyzed to determine the elemental abundance for the irradiated spots. The approach to demonstrating a proof of concept of the AEXS has been through 1) demonstrating the viability of microfabricated membranes, 2) assembling AEXS setups with increasingly integrated functional components, and 3) simulating the AEXS observational capabilities. The development of the instrument is described in detail in the poster paper[4] at this conference. This paper focuses on describing the progress of the AEXS instrument to acquire XRF data and using commercially available software to analyze the data streams and determine the accuracy, precision and resolution of the analysis compared to the certified elemental abundance.

Urgiles, E.↗

NASA NTRSDate not supplied

Open-source Wireless Sensor Network (Wi-Se Net) for Flexible Deployment

Wireless sensors, especially if battery powered, have a number of advantages over wired sensors for flexible or temporary diagnostic deployment in a field or lab setting. Recent advances in wireless technology and microprocessor boards have produced a variety of inexpensive off-the shelf chips which can communicate wirelessly with simple protocols. This paper describes the design and implementation of a highly customizable wireless sensor network (called WiSe Net) using inexpensive open-source hardware components as wireless nodes. These wireless sensor nodes can transmit data at a rate <250 Hz, can be battery powered, and have a small footprint (2x5 cm). In addition, a preliminary over-the-air programming system was developed to allow for simple wireless configuration when active. The network performance was demonstrated by taking distributed and electrically isolated temperature measurements on a high-voltage lab apparatus. Although this test case is in a laboratory setting, this network architecture could be easily repurposed for various other forms of monitoring

Wireless Sensor Network↗

NASA NTRSDate not supplied

TOWARD A METHOD FOR SCALING HUMAN BODY MODELS IN AN IMU-BASED WORKFLOW

BACKGROUND Scaled biomechanical models can more accurately inform crew health decisions when tailored to the wide range of astronaut sizes. One component to improve scaling of existing models to better represent each unique astronaut’s size is the individual length scaling of limbs. Traditionally, these lengths are determined by motion capture or manual measurement. A new method is herein proposed for length scaling which can be done by measuring linear and angular accelerations at a desired point during isolated motion around a point of rotation, then calculating the distance between the desired point and point of rotation. When an Inertial Measurement Unit (IMU) device is placed at the distal point of a limb, the isolated motion is about that limb’s proximal joint. For example, to measure forearm length, an IMU is placed at the wrist, the point of rotation is at the elbow, and the isolated motion is forearm flexion and extension. These calculated lengths are then used to scale models to each unique astronaut’s size, thereby improving the applicability of the model. This method of scaling limb segments can be used for any limb that has an easily defined proximal joint for the limb to rotate around including hands, arms, legs, feet. Utilizing IMUs for data collection also provides the synergistic ability to record data without a dedicated space in a room with many cameras, therefore reducing the data collection footprint, or record data where optical motion capture is not possible, such as inside a spacesuit. METHODS AND RESULTS To test this method, upper body data collection was performed with 5 Xsens DOT IMUs on a single subject. IMUs consist of an accelerometer, a gyroscope, and a magnetometer which collect linear acceleration, angular velocity, and magnetic fluctuations, respectively. Before any ground-based laboratory collection, the magnetic fluctuations are used to correct the heading of the IMU in space relative to the Earth’s magnetic field. The direct measurement of angular velocity is integrated to calculate angular acceleration. Then the linear acceleration ( a ) and angular acceleration (α) are solved using r = at/α to calculate the radius, which in this case is the distance between the IMU and the point of rotation (i.e., segment length). The IMU must be placed at the most distal point of the limb being measured (i.e., ankle if measuring lower leg length) and the test plan must consist of an isolated motion about that limb’s proximal joint (i.e., knee flexion and extension if measuring lower leg length). The distances (radii) calculated at every time interval were filtered (bandpass filter keeping 5th-90th percentile data) to eliminate outliers and spurious data that occur when the isolated motion was stopped or nearly stopped. The remaining distances were averaged, resulting in the calculated limb length. Scaling factors were then computed by dividing the calculated limb length by the unscaled model’s length. These scale factors are plugged into the Scale Tool in OpenSim [1,2] to apply the scaling to the OpenSim Full Body Rajagopal Model [3,4]. Manual measurements of limb lengths were taken before data collection started and used for comparing against the calculated lengths. The Anthropometric Survey of US Army Personnel (ANSUR II) [5] was also used as a third source of reference for limb length measurements. The following measurements were retrieved from the subject before data collection: 34.5 cm from L1 to C7 (thorax), 25.7 cm from C7 Joint Center (JC) to head vertex (neck and head), 36.3 cm from shoulder JC to elbow JC (humerus), 29.5 cm from elbow JC to wrist JC (forearm), and 16.2 cm from clavicle to acromion (clavicle). Of those five, forearm and humerus lengths were calculated using this proposed method to obtain preliminary results. The forearm length after filtering and averaging was calculated to be 37.6 cm. This is a 28% overestimation from the measured forearm length (29.5 cm). The humerus length after filtering and averaging was calculated to be 47.8 cm. This is a 31% difference from the measured subject length (36.3 cm). Sources of error include imperfect isolated motion (method currently expects that motion should be perfectly circular in a 2D plane, include no rotation of the IMU, and be relatively smooth; a more secure IMU attachment method will help), unrefined filter techniques (removed highest and lowest values with 20% high and low pass filters and no smoothing filters), arbitrary removal of stopped or near stopped data (kept data for only a short range before and after the angular velocity peaking), and a more representative method for removing gravitational acceleration is needed (current method is to zero all accelerations against a baseline taken just before the isolated motion which does not account for the gravitational acceleration changed due to IMU rotation during movement). Addressing these error sources will improve the accuracy of the limb length calculation. Next steps include creating a method for whole-body scaling estimation using individual limb scale factors. Continued pursuit of these techniques is expected to enable acquiring anthropometric information using only IMUs in real-time.

E. K. Marecki↗

NASA NTRSDate not supplied

Open-source Wireless Sensor Network (Wi-Se Net) for Flexible Deployment

Wireless sensors, especially if battery powered, have a number of advantages over wired sensors for flexible or temporary diagnostic deployment in a field or lab setting. Recent advances in wireless technology and microprocessor boards have produced a variety of inexpensive off-the shelf chips which can communicate wirelessly with simple protocols. This paper describes the design and implementation of a highly customizable wireless sensor network (called Wi-Se Net) using inexpensive open-source hardware components as wireless nodes. These wireless sensor nodes can transmit data at a rate <250 Hz, can be battery powered, and have a small footprint (2x5 cm). In addition, a preliminary over-the-air programming system was developed to allow for simple wireless configuration when active. The network performance was demonstrated by taking distributed and electrically isolated temperature measurements on a high-voltage lab apparatus. Although this test case is in a laboratory setting, this network architecture could be easily repurposed for various other forms of monitoring.

Wireless Sensor Network↗

NASA NTRSDate not supplied

Open-source Wireless Sensor Network (Wi-Se Net) for Flexible Deployment

Wireless Sensor Network↗

NASA NTRSDate not supplied

Toward A Method for Scaling Human Body Models in an IMU-Based Workflow

- Scaled biomechanical models can more accurately inform crew health decisions when tailored to the wide range of astronaut sizes. One component to improve scaling of existing models to better represent each unique astronaut’s size is the individual length scaling of limbs. Traditionally, limb lengths are determined by motion capture or manual measurement. - A new method is herein proposed for length scaling which can be done by measuring linear and angular accelerations at a desired point during isolated motion around a point of rotation, then calculating the distance between the desired point and point of rotation. - When an Inertial Measurement Unit (IMU) device is placed at the distal point of a limb, the isolated motion is about that limb’s proximal joint. This method of scaling limb segments can be used for any limb that has an easily defined proximal joint for the limb to rotate around including hands, arms, legs, feet. - Calculated limb lengths are then used to scale models to each unique astronaut’s size, thereby improving the applicability of the model. - This method was investigated as a possible away to obtain scaling information in data collections where IMUs are worn, but optical motion capture may not always be available, such as inside spacesuits or during crew exercise on the International Space Station.

E. K. Marecki↗

DOE OSTI2025

Accurate noncovalent interactions in atomistic systems via quantum Drude oscillators

Accurately modeling polarization and van der Waals (vdW) interactions in atomistic systems typically requires high-level quantum-mechanical methods that are computationally expensive, hence limited in applicability. To address this challenge, efficient yet physically grounded models are needed—ones that not only enable accurate predictions but also provide insight into how noncovalent interactions scale in complex molecular and material systems. This review highlights the quantum Drude oscillator (QDO) model, a physically motivated and computationally efficient framework that captures the essential features of electronic response, including polarization and dispersion forces, across a wide range of chemical and material systems. We discuss how the QDO model quantitatively reproduces the polarization response of many-electron atoms and how key components of noncovalent interactions—exchange-repulsion, polarization, and dispersion—emerge naturally in QDO dimers. Furthermore, the model provides predictive scaling laws that elucidate trends in polarizability and dispersion across the periodic table and in molecular assemblies. By uniting interpretability, accuracy, and efficiency, the QDO model offers a versatile approach for modeling noncovalent interactions in systems ranging from isolated molecules to complex condensed phases and nanostructured materials.

Khabibrakhmanov, Almaz [Univ. of Luxembourg, Luxem↗

DOE OSTI2021

A multi-omics approach to lignocellulolytic enzyme discovery reveals a new ligninase activity from Parascedosporium putredinis NO1

Lignocellulose, the structural component of plant cells, is a major agricultural byproduct and the most abundant terrestrial source of biopolymers on Earth. The complex and insoluble nature of lignocellulose limits its conversion into value-added commodities, and currently, efficient transformation requires expensive pretreatments and high loadings of enzymes. In this work, we report on a fungus from the Parascedosporium genus, isolated from a wheat-straw composting community, that secretes a large and diverse array of carbohydrate-active enzymes (CAZymes) when grown on lignocellulosic substrates. We describe an oxidase activity that cleaves the major β-ether units in lignin, thereby releasing the flavonoid tricin from monocot lignin and enhancing the digestion of lignocellulose by polysaccharidase mixtures. We show that the enzyme, which holds potential for the biorefining industry, is widely distributed among lignocellulose-degrading fungi from the Sordariomycetes phylum.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2025

Impact of Irradiation on Corrosion Performance of Hybrid Organic/Inorganic Coatings on Austenitic Stainless Steel

The effects of gamma radiation on the performance of two corrosion-resistant coatings applied to stainless-steel 304L (SS304L) surfaces are presented. Specifically, the ability of the coatings to mitigate corrosion of SS304L surfaces as a function of the dose received (0–1300 Mrad) and dose rate (176 compared to 1054 rad/s) is evaluated using electrochemical methods, spectroscopy, and microscopy. Coating A, an organic/inorganic hybrid coating consisting of a two-part silica ceramic component and a polymer linker was evaluated in comparison to Coating B, which utilized Coating A as a topcoat for a commercial, off-the-shelf, Zn-rich primer. Post irradiation, Coating A demonstrated some corrosion protection following exposure to low levels of gamma radiation, but coating degradation occurred with an increased exposure dose and resulted in isolated regions of corrosion initiation. For Coating B, greater corrosion resistance was observed compared to Coating A due to the sacrificial nature of the Zn at elevated doses of gamma radiation. No effect of the dose rate (for the single dose examined) was observed for either coating. It is proposed for Coating B that as the polymer coating thermally degrades above 250 °C (bond scission of the polymer occurs), the remaining Zinc layer adhered to the SS304L post-irradiation enables enhanced corrosion resistance as compared to Coating A, which displays solely polymer degradation. The results presented herein establish an understanding of coating behavior with radiation exposure, specifically the relationship between corrosion coating performance and radiation dose, and can inform ageing and lifetime management for various applications.

degradation↗

NASA NTRS1978

A method for calculating strut and splitter plate noise in exit ducts: Theory and verification

Portions of a four-year analytical and experimental investigation relative to noise radiation from engine internal components in turbulent flow are summarized. Spectra measured for such airfoils over a range of chord, thickness ratio, flow velocity, and turbulence level were compared with predictions made by an available rigorous thin-airfoil analytical method. This analysis included the effects of flow compressibility and source noncompactness. Generally good agreement was obtained. This noise calculation method for isolated airfoils in turbulent flow was combined with a method for calculating transmission of sound through a subsonic exit duct and with an empirical far-field directivity shape. These three elements were checked separately and were individually shown to give close agreement with data. This combination provides a method for predicting engine internally generated aft-radiated noise from radial struts and stators, and annular splitter rings. Calculated sound power spectra, directivity, and acoustic pressure spectra were compared with the best available data. These data were for noise caused by a fan exit duct annular splitter ring, larger-chord stator blades, and turbine exit struts.

Fink, M. R.↗

NASA NTRS1995

Summary Report of Mission Acceleration Measurements for STS-65, Launched 8 July 1994

The second flight of the International Microgravity Laboratory (IML-2) payload on board the STS-65 mission was supported by three accelerometer instruments: The Orbital Acceleration Research Experiment (OARE) located close to the orbiter center of mass; the Quasi-Steady Acceleration Measurement experiment, and the Space Acceleration Measurement System (SAMS), both in the Spacelab module. A fourth accelerometer, the Microgravity Measuring Device recorded data in the middeck in support of exercise isolation tests.Data collected by OARE and SAMS during IML-2 are displayed in this report. The OARE data represent the microgravity environment below 1 Hz. The SAMS data represent the environment in the 0.01 Hz to 100 Hz range. Variations in the environment caused by unique activities are presented. Specific events addressed are: crew activity, crew exercise, experiment component mixing activities, experiment centrifuge operations, refrigerator/freezer operations and circulation pump operations. The analyses included in this report complement analyses presented in other mission summary reports.

Rogers, Melissa J. B.↗

NASA NTRS1998

Relationship of Topside Ionospheric Ion Outflows to Auroral Forms and Precipitation, Plasma Waves, and Convection Observed by Polar

The POLAR satellite often observes upflowing ionospheric ions (UFIs) in and near the aurora] oval on southern perigee (approx. 5000 km altitude) passes. We present the UFI features observed by the thermal ion dynamics experiment (TIDE) and the toroidal imaging mass angle spectrograph (TIMAS) in the dusk-dawn sector under two different geomagnetic activity conditions in order to elicit their relationships with auroral forms, wave emissions, and convection pattern from additional POLAR instruments. During the active interval, the ultraviolet imager (UVI) observed a bright discrete aurora on the duskside after the substorm onset and then observed a small isolated aurora form and diffuse auroras on the dawnside during the recovery phase. The UFIs showed clear conic distributions when the plasma wave instrument (PWI) detected strong broadband wave emissions below approx. 10 kHz, while no significant auroral activities were observed by UVI. At higher latitudes, the low-energy UFI conics gradually changed to the polar wind component with decreasing intensity of the broadband emissions. V-shaped auroral kilometric radiation (AKR) signatures observed above -200 kHz by PWI coincided with the region where the discrete aurora and the UFI beams were detected. The latitude of these features was lower than that of the UFI conics. During the observations of the UFI beams and conics, the lower-frequency fluctuations observed by the electric field instrument were also enhanced, and the convection directions exhibited large fluctuations. It is evident that large electrostatic potential drops produced the precipitating electrons and discrete auroras, the UFI beams, and the AKR, which is also supported by the energetic plasma data from HYDRA. Since the intense broadband emissions were also observed with the UFIs, the ionospheric ions could be energized transversely before or during the parallel acceleration due to the potential drops.

Hirahara, M.↗

NASA NTRS2010

Isolation of Resistance-Bearing Microorganisms

To better exploit the principles of gas transport and mass transport during the processes of cell seeding of 3D scaffolds and in vitro culture of 3D tissue engineered constructs, the oscillatory cell culture bioreactor provides a flow of cell suspensions and culture media directly through a porous 3D scaffold (during cell seeding) and a 3D construct (during subsequent cultivation) within a highly gas-permeable closed-loop tube. This design is simple, modular, and flexible, and its component parts are easy to assemble and operate, and are inexpensive. Chamber volume can be very low, but can be easily scaled up. This innovation is well suited to work with different biological specimens, particularly with cells having high oxygen requirements and/or shear sensitivity, and different scaffold structures and dimensions. The closed-loop changer is highly gas permeable to allow efficient gas exchange during the cell seeding/culturing process. A porous scaffold, which may be seeded with cells, is fixed by means of a scaffold holder to the chamber wall with scaffold/construct orientation with respect to the chamber determined by the geometry of the scaffold holder. A fluid, with/without biological specimens, is added to the chamber such that all, or most, of the air is displaced (i.e., with or without an enclosed air bubble). Motion is applied to the chamber within a controlled environment (e.g., oscillatory motion within a humidified 37 C incubator). Movement of the chamber induces relative motion of the scaffold/construct with respect to the fluid. In case the fluid is a cell suspension, cells will come into contact with the scaffold and eventually adhere to it. Alternatively, cells can be seeded on scaffolds by gel entrapment prior to bioreactor cultivation. Subsequently, the oscillatory cell culture bioreactor will provide efficient gas exchange (i.e., of oxygen and carbon dioxide, as required for viability of metabolically active cells) and controlled levels of fluid dynamic shear (i.e., as required for viability of shear-sensitive cells) to the developing engineered tissue construct. This bioreactor was recently utilized to show independent and interactive effects of a growth factor (IGF-I) and slow bidirectional perfusion on the survival, differentiation, and contractile performance of 3D tissue engineering cardiac constructs. The main application of this system is within the tissue engineering industry. The ideal final application is within the automated mass production of tissue- engineered constructs. Target industries could be both life sciences companies as well as bioreactor device producing companies.

Venkateswaran, Kasthuri, J.↗