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At least 1,045 records · Page 58

Snow and Water Imaging Spectrometer: Final Instrument Characterization

The Snow and Water Imaging Spectrometer (SWIS) is a science-grade imaging spectrometer and telescope system suitable for CubeSat applications, spanning a 350-1700 nm spectral range with 5.7 nm sampling, a 10 degree field of view and 0.3 mrad spatial resolution. The system operates at F/1.8, providing high throughput for low-reflectivity water surfaces, while avoiding saturation over bright snow or clouds. The SWIS design utilizes heritage from previously demonstrated instruments on airborne platforms, while advancing the state of the art in compact sensors of this kind in terms of size and spectral coverage. Through frequent repeat observations from space at a moderate spatial resolution, SWIS can address key science questions concerning aquatic and terrestrial ecosystem changes, cryosphere warming and melt behavior, cloud and atmospheric science, and potential impacts of climate change and human activities on the environment. We review the optical design and innovations and key technologies developed for this instrument, as well as its measured optical performance. We discuss the radiometric calibration characterization, including detector linearity, flat field correction, and SNR. Finally, we discuss stray light modeling and the development of a focused ghost removal algorithm, which is tested and supported by laboratory results.

Bellardo, John↗

A Pipeline for Assessing the Quality of Rna-Seq Datasets in GeneLab

Transcriptome profiling by RNA sequencing (RNA-seq) is a powerful approach to identify gene expression changes in organisms exposed to unique environments such as spaceflight. One of the challenges of evaluating RNA-seq data both within and across different space-relevant studies is the ability to control for technical differences, including the use of different library preparation kits, sequencing platforms, RNA yield, and person-to-person variation. To help address this issue, the National Institute of Standards and Technology (NIST, nist.gov) initiated a consortium, at the request of industry and academia, to develop a set of controls for gene expression measurements. The result was a set of 92 unlabeled, polyadenylated transcripts that range from 250 – 2,000 nucleotides in length to mimic natural eukaryotic mRNAs. These External RNA Controls Consortium (ERCC) genes can be used in any RNA-seq experiment, by adding known concentrations of the ERCC genes to samples after RNA extraction, to offer a standard measurement for data comparison. At NASA GeneLab, we employ these controls as part of our standard operating procedures for every in-house RNA-seq study to assess the limit of detection, dynamic range, and power of differential expression analysis both within and across experiments. Here we will discuss the use, benefits, and limitations of ERCC genes and other types of controls, such as universal RNA references, to generate quality control information for RNA-seq studies conducted at GeneLab.

GeneLab↗

Optimizing a Small RNAseq Analysis Pipeline for NASA GeneLab Using Open-Source Tools and Libraries

Small RNA sequencing (small RNAseq) is a powerful tool for studying the regulation of gene expression in various organisms. Small RNAseq has been leveraged in space biology research to study how expression of small RNAs, e.g. micro RNAs (miRNAs), small interfering RNAs (siRNAs), and piwi-interacting RNAs (piRNAs), change upon exposure to the space environment. NASA GeneLab currently hosts small RNAseq raw data derived from space-relevant experiments on the Open Science Data Repository (OSDR). To maximize the accessibility of these data to the scientific community, in addition to hosting raw data, which is only interpretable by bioinformaticians, GeneLab plans to process all small RNAseq datasets and make those processed data available to the scientific community via the OSDR. In this study, we present the development of the GeneLab standardized pipeline for processing small RNAseq datasets. Using human, plant, and synthetic small RNAseq datasets, we interrogate various open-source software and publicly available databases to evaluate their accuracy and reproducibility in each step of the pipeline. For quality control and adapter detection and trimming, we evaluated TrimGalore!, FASTX, SeqKit, and DNApi methods to optimize alignment to reference genomes. We compared BWA, Bowtie, and Bowtie2 to determine the optimal alignment tool. For each alignment tool we also assessed various reference databases, including Ensembl reference genomes and different types of small RNA reference databases, including genome, hairpin, and miRNA references from the miRbase and MirGeneDB databases. To quantify the aligned data, we compared SAMtools, HTSeq, and RSEM for counting alignment events from each alignment tool used. Finally, we evaluated various tools, including DESeq2 and EdgeR, for data normalization and subsequent differential expression analysis. We will present the results from our comparative analyses for each pipeline step and propose a consensus pipeline for processing small RNAseq data derived from various organisms exposed to the space environment.

SmallRNAseq, NASA GeneLab, quality control, adapte↗

Earth-Independent Medical Operations (EIMO) Concept of Operations

In contrast to the current crew health paradigm for low-Earth orbit and Lunar missions, which depends on real-time communication with Mission Control, deep-space exploration missions will require a significant shift in medical operations. This shift is driven by the constraints of operating at a considerable distance from Earth, such as resource limitations—lack of resupply, restricted mass, power, volume, and data—as well as communication delays and the inability to evacuate back to Earth during emergencies. To move toward a more self-reliant medical model, a strategy is needed to gradually increase space-based crew autonomy and reduce risks to mission success in the challenging environment of deep space. This transformative change, known as "Earth-Independent Medical Operations" (EIMO), explores the gradual transfer of medical care and decision-making from Earth-based support to space-based systems. The goal of this transition is to enhance astronaut health and performance while minimizing mission risks. EIMO requires the development of a medical system that integrates seamlessly with mission planning, vehicle and spacesuit design, and data architecture. This integration is crucial for building a robust medical infrastructure that not only safeguards astronaut well-being but also ensures overall mission success. The Human Research Program (HRP) Exploration Medical Capability (ExMC) Element has revised the EIMO model-based Concept of Operations (ConOps) which outlines an initial vision for EIMO. The ConOps, which is built on the stakeholders’ need, system goals, and objectives (NGOs), presents an array of in-mission scenarios that span a wide range of medical conditions demonstrating the system’s capabilities from basic to complex events. Developed by a multidisciplinary team of systems engineers, scientists, and clinicians within ExMC, the ConOps revision includes two new scenarios(Barotrauma and Self-Medical Management and Behavioral Health and Chronic Medical Care), and implementation of findings from EIMO technical interchange meetings that focused on data and training. The envisioned EIMO Medical System (MS) operates as a system of systems, gathering data from various sources such as reference databases, real-time wearable sensors, point-of-care diagnostics, and environmental controls. The MS also incorporates advanced training tools to support autonomous medical care, assisting the Crew Medical Officer (CMO) during medical events where Ground Support is either unavailable or communication-delayed beyond practicality. Furthermore, MS functions and capabilities were decomposed from the scenarios to establish foundational requirements for EIMO and traced to the NASA Spaceflight Human-System Standard(NASA-STD-3001, Volumes 1 and 2). These traces were performed to gain insights on the alignment of EIMO requirements with the NASA standard. This work serves as an initial recommendation to increase crew autonomy gradually and safely for Mars missions and future deep-space exploration.

medical system↗

Sensitivity of Space Launch System Buffet Forcing Functions to Buffet Mitigation Options

Time-varying buffet forcing functions arise from unsteady aerodynamic pressures and are one of many load environments, which contribute to the overall loading condition of a launch vehicle during ascent through the atmosphere. The buffet environment is typically highest at transonic conditions and can excite the vehicle dynamic modes of vibration. The vehicle response to these buffet forcing functions may cause high structural bending moments and vibratory environments, which can exceed the capabilities of the structure, or of vehicle components such as payloads and avionics. Vehicle configurations, protuberances, payload fairings, and large changes in stage diameter can trigger undesirable buffet environments. The Space Launch System (SLS) multi-body configuration and its structural dynamic characteristics presented challenges to the load cycle design process with respect to buffet-induced loads and responses. An initial wind-tunnel test of a 3-percent scale SLS rigid buffet model was conducted in 2012 and revealed high buffet environments behind the booster forward attachment protuberance, which contributed to reduced vehicle structural margins. Six buffet mitigation options were explored to alleviate the high buffet environments including modified booster nose cones and fences/strakes on the booster and core. These studies led to a second buffet test program that was conducted in 2014 to assess the ability of the buffet mitigation options to reduce buffet environments on the vehicle. This paper will present comparisons of buffet forcing functions from each of the buffet mitigation options tested, with a focus on sectional forcing function rms levels within regions of the vehicle prone to high buffet environments.

Piatak, David J.↗

LASSIE: Legged Autonomous Surface Science In Analogue Environments

Roving planetary surface exploration missions operate using a pre-programmed plan that can limit the capability to effectively detect unexpected changes in terrain properties, adjust locomotion or sampling strategies, and autonomously identify scientifically valuable observations and adjust exploration strategies. In particular, the inability to measure and react to unexpected changes in regolith properties can negatively impact mission operations. The Mars Exploration Rover Spirit and InSight lander both experienced challenges related to understanding the geotechnical properties of regolith. The advancements in legged robotic platforms hold potential to address these challenges through greater sensitivity to changing surface properties. The Legged Autonomous Surface Science In Analogue Environments (LASSIE) project explores how legged roving platforms can use the leg motors to measure geotechnical properties of crusted and icy surface regolith and utilize those measurements to autonomously update the science operations plans. These tests will be performed in martian and lunar analog environments and also within lab settings.

K. R. Fisher↗

A Database of Interplanetary and Interstellar Dust Detected by the Wind Spacecraft

It was recently discovered that the WAVES instrument on the Wind spacecraft has been detecting, in situ, interplanetary and interstellar dust of approximately 1 micron radius for the past 22 years. These data have the potential to enable advances in the study of cosmic dust and dust-plasma coupling within the heliosphere due to several unique properties: the Wind dust database spans two full solar cycles; it contains over 107,000 dust detections; it contains information about dust grain direction of motion; it contains data exclusively from the space environment within 350 Earth radii of Earth; and it overlaps by 12 years with the Ulysses dust database. Further, changes to the WAVES antenna response and the plasma environment traversed by Wind over the lifetime of the Wind mission create an opportunity for these data to inform investigations of the physics governing the coupling of dust impacts on spacecraft surfaces to electric field antennas. A Wind dust database has been created to make the Wind dust data easily accessible to the heliophysics community and other researchers. This work describes the motivation, methodology, contents, and accessibility of the Wind dust database.

interplaneary dust↗

Simulation and Optimization Methods for Assessing the Impact of Aviation Operations on the Environment

There is increased awareness of anthropogenic factors affecting climate change and urgency to slow the negative impact. Greenhouse gases, oxides of Nitrogen and contrails resulting from aviation affect the climate in different and uncertain ways. This paper develops a flexible simulation and optimization software architecture to study the trade-offs involved in reducing emissions. The software environment is used to conduct analysis of two approaches for avoiding contrails using the concepts of contrail frequency index and optimal avoidance trajectories.

Sridhar, Banavar↗

Evaluation of space environmental effects on metals and optical thin films on EOIM-3

Metals and optical thin films exposed to the space environment on the Third Flight of the Evaluation of Oxygen Interactions with Materials (EOIM-3) payload, onboard Space Shuttle mission STS-46 were evaluated. The materials effects described in this paper include the effects of space exposure on various pure metals, optical thin films, and optical thin film metals. The changes induced by exposure to the space environment in the material properties were evaluated using bidirectional reflectance distribution function (BRDF), specular reflectance (250 nm to 2500 nm), ESCA, VUV reflectance (120 nm to 200 nm), ellipsometry, FTIR and optical properties. Using these analysis techniques gold optically thin film metal mirrors with nickel undercoats were observed to darken due to nickel diffusion through the gold to the surface. Also, thin film nickel mirrors formed nickel oxide due to exposure to both the atmosphere and space.

Vaughn, Jason A.↗

Water Based Phase Change Material Heat Exchanger Development

In a cyclical heat load environment such as low Lunar orbit, a spacecraft’s radiators are not sized to reject the full heat load requirement. Traditionally, a supplemental heat rejection device (SHReD) such as an evaporator or sublimator is used to act as a “topper” to meet the additional heat rejection demands. Utilizing a Phase Change Material (PCM) heat exchanger (HX) as a SHReD provides an attractive alternative to evaporators and sublimators as PCM HXs do not use a consumable, thereby leading to reduced launch mass and volume requirements. Studies conducted in this paper investigate utilizing water’s high latent heat of formation as a PCM, as opposed to traditional waxes, and corresponding complications surrounding freezing water in an enclosed volume. Work highlighted in this study is primarily visual and includes understanding ice formation, freeze front propagation, and the solidification process of water/ice. Various test coupons were constructed of copper to emulate the interstitial pin configuration (to aid in conduction) of the proposed water PCM HX design. Construction of a prototypic HX was also completed in which a flexible bladder material and interstitial pin configurations were tested. Additionally, a microgravity flight was conducted where three copper test articles were frozen continuously during microgravity and 2-g periods and individual water droplets were frozen during microgravity.

Hansen, Scott W.↗

Continued Water-Based Phase Change Material Heat Exchanger Development

In a cyclical heat load environment such as low Lunar orbit, a spacecraft's radiators are not sized to reject the full heat load requirement. Traditionally, a supplemental heat rejection device (SHReD) such as an evaporator or sublimator is used to act as a "topper" to meet the additional heat rejection demands. Utilizing a Phase Change Material (PCM) heat exchanger (HX) as a SHReD provides an attractive alternative to evaporators and sublimators as PCM HXs do not use a consumable, thereby leading to reduced launch mass and volume requirements. In continued pursuit of water PCM HX development two full‐scale, Orion sized water‐based PCM HX's were constructed by Mezzo Technologies. These HX's were designed by applying prior research and experimentation to the full scale design. Design options considered included bladder restraint and clamping mechanisms, bladder manufacturing, tube patterns, fill/drain methods, manifold dimensions, weight optimization, and midplate designs. Design and construction of these HX's led to successful testing of both PCM HX's.

Hansen, Scott↗

Continued Water-Based Phase Change Material Heat Exchanger Development

In a cyclical heat load environment such as low Lunar orbit, a spacecraft's radiators are not sized to meet the full heat rejection demands. Traditionally, a supplemental heat rejection device (SHReD) such as an evaporator or sublimator is used to act as a "topper" to meet the additional heat rejection demands. Utilizing a Phase Change Material (PCM) heat exchanger (HX) as a SHReD provides an attractive alternative to evaporators and sublimators as PCM HX's do not use a consumable, thereby leading to reduced launch mass and volume requirements. In continued pursuit of water PCM HX development two full-scale, Orion sized water-based PCM HX's were constructed by Mezzo Technologies. These HX's were designed by applying prior research on freeze front propagation to a full-scale design. Design options considered included bladder restraint and clamping mechanisms, bladder manufacturing, tube patterns, fill/drain methods, manifold dimensions, weight optimization, and midplate designs. Two units, Units A and B, were constructed and differed only in their midplate design. Both units failed multiple times during testing. This report highlights learning outcomes from these tests and are applied to a final sub-scale PCM HX which is slated to be tested on the ISS in early 2017.

Hansen, Scott W.↗

Going beyond reliability to robustness and resilience in space systems

The words reliability, robustness, and resilience, are often used interchangeably to describe tough and dependable systems but the distinctions between them suggest how to design more serviceable space systems. Reliability is simply the quality of consistently performing well. A system that dependably meets its design requirements in the specified environments is reliable. The designers may not consider themselves responsible for failures under unanticipated conditions. Robustness is the capability of performing without failure under a wide range of conditions, which can go beyond the expected range to include possible off-nominal conditions. Resilience is the ability to recover from or adapt to damaging events, such as failures, accidents, external disruptions, and repurposing. Such changes are usually unanticipated. They often invalidate the usual operating assumptions and cause system failure. Reliability, robustness, and resilience describe dependable performance under increasingly difficult conditions, first the specified environment, then a wider possible environment, and finally unanticipated damaging events. These three are increasingly desirable and increasingly difficult to achieve. Engineering for resilience would design systems that can ignore or repair failures, survive accidents, and recover from disruptions. Increasing the resilience of space systems, the ability to perform after unanticipated events, would greatly increase space crew safety. Improving reliability and robustness can be done by dealing with known sources of problems, but improving resilience requires implementing a general approach to reducing the impact of unknown future events. Two contrasting approaches are reducing system complexity and adding supervisory control. The need for resilience has been claimed for decades but little has been accomplished. Systems designers assume that they understand requirements, technologies, designs, architectures, integration, testing, operations, and environments. The potential problems of changes, failures, accidents, unknown environments, and unknown unknowns are ignored. Systems designers are typically overconfident and ignore the need for robustness and resilience.

Harry W Jones↗

An Agent-Based Interface to Terrestrial Ecological Forecasting

The latest generation of NASA Earth Observing System (EOS) satellites has brought a new dimension to continuous monitoring of the living part of the Earth System, the biosphere. EOS data can now provide weekly global measures of vegetation productivity and ocean chlorophyll, and many related biophysical factors such as land cover changes or snowmelt rates. However, the highest economic value would come from forecasting impending conditions of the biosphere, to allow decision makers to mitigate dangers or exploit positive trends. NASA's strategic plan for the Earth Science Enterprise i d e n a s ecological forecasting as a focus for research. Ecological forecasting predicts the effects of changes in the physical, chemical and biological environment on ecosystem activity. Possible applications of such a system include predicting shortfalls or bumper crops of agricultural production, populations of threatened or invasive species or wildfire danger in time to allow improves preparation and logistical efficiency. Petabytes of remote sensing data are now available to help measure, understand and forecast changes in the Earth system, but using these data effectively can be surprisingly hard. The volume and variety of data files and formats are daunting. Simple data management activities, such as locating and transferring files, changing file formats, gridding point data, and scaling and reprojecting gridded data, can consume far more personnel time and resources than the actual data analysis. Some scientists commit to a particular data source or resolution just because using anything different would be more effort that it's worth. Better tools can help, but most of the tools developed to date are little more than shell scripts; they lack the flexibility to meet the diverse needs of users and are difficult to extend to handle changes in available data sources.

Golden, Keith↗

In Situ Fiber-Optic Reflectance Monitor

In situ fiber-optic reflectance monitor serves as simple means of monitoring changes in reflectance of specimen exposed to simulated outerspace or other environments in vacuum chamber. Eliminates need to remove specimen from vacuum chamber, eliminating optical changes and bleaching such removal causes in coatings.

Linton, Roger C.↗

Assessment of Urbanization on the Integrated Land-Ocean-Atmosphere Environment in Coastal Metropolis in Preparation for HyspIRI

The need for comprehensive studies of the relationships between past and projected changes of regional climate and human activity in comple x urban environments has been well established. The HyspIRI preparato ry airborne activities in California, associated science and applicat ions research, and eventually HyspIRI itself provide an unprecedented opportunity for development and implementation of an integrated data and modeling analysis system focused on coastal urban environments. We will utilize HyspIRI preparatory data collections in developing ne w remote sensing-based tools for investigating the integrated urban e nvironment, emphasizing weather, climate, and energy demands in compl ex coastal cities.

Sequera, Pedro↗

Statistical Analysis of Large Simulated Yield Datasets for Studying Climate Effects

Many studies have been carried out during the last decade to study the effect of climate change on crop yields and other key crop characteristics. In these studies, one or several crop models were used to simulate crop growth and development for different climate scenarios that correspond to different projections of atmospheric CO2 concentration, temperature, and rainfall changes (Semenov et al., 1996; Tubiello and Ewert, 2002; White et al., 2011). The Agricultural Model Intercomparison and Improvement Project (AgMIP; Rosenzweig et al., 2013) builds on these studies with the goal of using an ensemble of multiple crop models in order to assess effects of climate change scenarios for several crops in contrasting environments. These studies generate large datasets, including thousands of simulated crop yield data. They include series of yield values obtained by combining several crop models with different climate scenarios that are defined by several climatic variables (temperature, CO2, rainfall, etc.). Such datasets potentially provide useful information on the possible effects of different climate change scenarios on crop yields. However, it is sometimes difficult to analyze these datasets and to summarize them in a useful way due to their structural complexity; simulated yield data can differ among contrasting climate scenarios, sites, and crop models. Another issue is that it is not straightforward to extrapolate the results obtained for the scenarios to alternative climate change scenarios not initially included in the simulation protocols. Additional dynamic crop model simulations for new climate change scenarios are an option but this approach is costly, especially when a large number of crop models are used to generate the simulated data, as in AgMIP. Statistical models have been used to analyze responses of measured yield data to climate variables in past studies (Lobell et al., 2011), but the use of a statistical model to analyze yields simulated by complex process-based crop models is a rather new idea. We demonstrate herewith that statistical methods can play an important role in analyzing simulated yield data sets obtained from the ensembles of process-based crop models. Formal statistical analysis is helpful to estimate the effects of different climatic variables on yield, and to describe the between-model variability of these effects.

climate↗

Exposure to Stressful Environments: Strategy of Adaptive Responses

Any new natural environment may generate a number of stresses (such as hypoxia, water lack, and heat exposure), each of which can produce strains in more than a single organ system. Every strain may in turn stimulate the body to adapt in multiple ways. Nevertheless, a general strategy of the various adaptive responses emerges when the challenges are divided into three groups. The first category includes conditions that affect the supply of essential molecules, while the second is made up by those stresses that prevent the body from regulating properly the output of waste products, such as CO2 and heat. In both classes, there is a small number of responses, similar in principle, regardless of the specific situation. The third unit is created by environments that disrupt body transport systems. Problems may arise when there is a conflict between two stresses requiring conflicting adaptive changes. An alternative to adaptation, creation of micro-environment, is often favored by the animal.

Farhi, Leon E.↗