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At least 1,027 records · Page 57

Genome collection processing for “Conserved upper thermal limits and small safety margins in soil copiotrophic bacteria”

We extracted the genomic DNA of 400 randomly selected isolates using a Quick-DNA Microprep Kit (Zymo Research D3020) according to the manufacturer’s protocol. We then submitted the extracted gDNA samples for short-read Illumina sequencing (200 Mbp) at SeqCoast Genomics (Portsmouth, NH, USA). After preprocessing the sequences using Trimmommatic (Bolger et al. 2014), we assembled the genomes using SPADES (Bankevich et al. 2012) and checked the quality of each assembly using QUAST (Gurevich et al. 2013). We processed the genome assemblies using a KBase (v1.4.0) pipeline (Allen et al. 2017; Arkin et al. 2018). Briefly, we used DRAM (v0.1.2) with default settings to annotate the genome assemblies. We then evaluated genome quality and possible contamination levels using CheckM (v1.0.18) (Parks et al. 2015) and retained genomes with completeness above 98% and contamination below 5% (n = 354), following the authors' guidelines. We then obtained taxonomic assignments for all remaining isolates using the Genome Taxonomy Database tool GTDB-Tk (v2.3.2, database version r214) (Chaumeil et al. 2019). We constructed a phylogenetic tree using the tool SpeciesTree (v2.2.0). We then trimmed the tree (using Trim SpeciesTree to GenomeSet- v1.4.0), retaining only tips within our collection with measured thermal performance.

59 BASIC BIOLOGICAL SCIENCES↗

Impact and extinction signatures in complete Cretaceous-Tertiary (K-T) boundary sections

The Zumaya, Caravaca and Agost sections in Spain, the El Kef section in Tunisia and the Negev (Nahal Avdat) sections in Israel are among the most continuous, expanded and complete K-T boundary sections. The distribution patterns of the planktic faunas were quantitatively analyzed in closely spaced samples across the K-T boundary in these sections, in conjuction with the geochemistry, stable isotopes, mineralogy and magnetostratigraphy. Three hundred foraminiferal specimens were randomly selected and determined. Reliable estimates for the foraminiferal productivity changes across the K-T boundary and for the 1 to 2 Ma interval preceding the K-T boundary were made from the numbers of individuals/gram of sediment corrected for the sedimentation rates (calculated from magnetic reversals and lithology). No gradual or stepwise extinction is seen below the K-T boundary nor any productivity decrease. Stable isotope analyses show a warming just after deposition of the ejecta layer, not cooling as predicted by nuclear winter scenarios, although the duration of such cooling may be too short to be observed even in these complete sections. Low REE values and cpx spherules with quench textures idential to quench-textures in diagenetically altered spherules, strongly indicate an oceanic site of (one of) the impactor(s).

Smit, J.↗

Search for partial systemic Lyman edges in nearby quasars

IUE archival spectra of nearby quasars with redshifts in the range 0.4-2.3 were searched for changes in the continuum at the systemic Lyman edge positions. Thirty-nine percent of the sample do not show any discontinuities. Twenty-nine percent of the sample show complete Lyman edges at redshifts less than the quasar redshift. These objects have associated narrow absorption lines; hence the intervening gas responsible for the absorption is extrinsic to the quasar and its environment. Twenty-two percent of the sample show Lyman edges at the quasar redshift with associated narrow absorption lines. In these objects the gas is associated with the AGN or its host galaxy. Ten percent of the sample have Lyman edges with no known associated absorption lines. If Lyman edge discontinuities are due to optically thick, geometrically thin accretion disks, which are randomly oriented to the line of sight, these results indicate that the Lyman edge discontinuity, either in emission or absorption, has to be not more than 15 percent in most quasars.

Koratkar, A. P.↗

A Closer Look at Non-Random Patterns Within Chemistry Space for a Smaller, Earlier Amino Acid Alphabet

Recent findings, in vitro and in silico, are strengthening the idea of a simpler, earlier stage of genetically encoded proteins which used amino acids produced by prebiotic chemistry. These findings motivate a re-examination of prior work which has identified unusual properties of the set of twenty amino acids found within the full genetic code, while leaving it unclear whether similar patterns also characterize the subset of prebiotically plausible amino acids. We have suggested previously that this ambiguity may result from the low number of amino acids recognized by the definition of prebiotic plausibility used for the analysis. Here, we test this hypothesis using significantly updated data for organic material detected within meteorites, which contain several coded and non-coded amino acids absent from prior studies. In addition to confirming the well-established idea that “late” arriving amino acids expanded the chemistry space encoded by genetic material, we find that a prebiotically plausible subset of coded amino acids generally emulates the patterns found in the full set of 20, namely an exceptionally broad and even distribution of volumes and an exceptionally even distribution of hydrophobicities (quantified as logP) over a narrow range. However, the strength of this pattern varies depending on both the size and composition the library used to create a background (null model) for a random alphabet, and the precise definition of exactly which amino acids were present in a simpler, earlier code. Findings support the idea that a small sample size of amino acids caused previous ambiguous results, and further improvements in meteorite analysis, and/or prebiotic simulations will further clarify the nature and extent of unusual properties. We discuss the case of sulfur-containing amino acids as a specific and clear example and conclude by reviewing the potential impact of better understanding the chemical “logic” of a smaller forerunner to the standard amino acid alphabet.

Amino acids↗

Fault Graph (fg)

This software package offers a novel fault graph modeling language and a simulation tool for executing models specified in the language. The modeling language has three primary features that distinguish it from similar reliability analysis tools. These are (1) a random variable modeling several distinct outcomes of a single fault; (2) chains of faults in which one fault triggers another; and (3) time to fail sampled from probability distributions including positive normal, exponential, Weibull with a minimum, or immediate.

Nutaro, James↗

Scheduling strategies for the ESPRESSO follow-up of TESS targets

ABSTRACT Radial-velocity follow-up of stars harbouring transiting planets detected by TESS is expected to require very large amounts of expensive telescope time in the next few years. Therefore, scheduling strategies should be implemented to maximize the amount of information gathered about the target planetary systems. We consider myopic and non-myopic versions of a novel uniform-in-phase scheduler, as well as a random scheduler, and compare these scheduling strategies with respect to the bias, accuracy and precision achieved in recovering the mass and orbital parameters of transiting and non-transiting planets. This comparison is carried out based on realistic simulations of radial-velocity follow-up with ESPRESSO of a sample of 50 TESS target stars, with simulated planetary systems containing at least one transiting planet with a radius below 4R⊕. Radial-velocity data sets were generated under reasonable assumptions about their noise component, including that resulting from stellar activity, and analysed using a fully Bayesian methodology. We find the random scheduler leads to a more biased, less accurate, and less precise, estimation of the mass of the transiting exoplanets. No significant differences are found between the results of the myopic and non-myopic implementations of the uniform-in-phase scheduler. With only about 22 radial velocity measurements per data set, our novel uniform-in-phase scheduler enables an unbiased (at the level of 1 per cent) measurement of the masses of the transiting planets, while keeping the average relative accuracy and precision around 16 per cent and 23 per cent, respectively. The number of non-transiting planets detected is similar for all the scheduling strategies considered, as well as the bias, accuracy and precision with which their masses and orbital parameters are recovered.

Cabona, L.↗

Modeling intrinsic galaxy alignment in the MICE simulation

The intrinsic alignment (IA) of galaxies is potentially a major limitation in deriving cosmological constraints from weak lensing surveys. In order to investigate this effect, we assign intrinsic shapes and orientations to galaxies in the light-cone output of the MICE simulation, spanning ~ 5000 deg 2 and reaching redshift Z = 1.4. This assignment is based on a semianalytic IA model that uses photometric properties of galaxies as well as the spin and shape of their host halos. Advancing on previous work, we include more realistic distributions of galaxy shapes and a luminosity-dependent galaxy-halo alignment. The IA model parameters are calibrated against COSMOS and BOSS LOWZ observations. The null detection of IA in observations of blue galaxies is accounted for by setting random orientations for these objects. We compare the two-point alignment statistics measured in the simulation against predictions from the analytical IA models NLA and TATT over a wide range of scales, redshifts, and luminosities for red and blue galaxies separately. We find that both models fit the measurements well at scales above 8h -1 Mpc, while TATT outperforms NLA at smaller scales. The IA parameters derived from our fits are in broad agreement with various observational constraints from red galaxies. Finally, we build a realistic source sample, mimicking DES Year 3 observations and use it to predict the IA contamination to the observed shear statistics. We find this prediction to be within the measurement uncertainty, which might be a consequence of the random alignment of blue galaxies in the simulation.

79 ASTRONOMY AND ASTROPHYSICS↗

Modeling freight mode choice using machine learning classifiers: a comparative study using Commodity Flow Survey (CFS) data

This study explores the usefulness of machine learning classifiers for modeling freight mode choice. We investigate eight commonly used machine learning classifiers, namely Naïve Bayes, Support Vector Machine, Artificial Neural Network, K-Nearest Neighbors, Classification and Regression Tree, Random Forest, Boosting and Bagging, along with the classical Multinomial Logit model. US 2012 Commodity Flow Survey data are used as the primary data source; we augment it with spatial attributes from secondary data sources. The performance of the classifiers is compared based on prediction accuracy results. The current research also examines the role of sample size and training-testing data split ratios on the predictive ability of the various approaches. In addition, the importance of variables is estimated to determine how the variables influence freight mode choice. The results show that the tree-based ensemble classifiers perform the best. Specifically, Random Forest produces the most accurate predictions, closely followed by Boosting and Bagging. With regard to variable importance, shipment characteristics, such as shipment distance, industry classification of the shipper and shipment size, are the most significant factors for freight mode choice decisions.

42 ENGINEERING↗

Optimal Eco-Driving Control of Connected and Autonomous Vehicles Through Signalized Intersections

This article focuses on the speed planning problem for connected and automated vehicles (CAVs) communicating to traffic lights. The uncertainty of traffic signal timing for signalized intersections on the road is considered. The eco-driving problem is formulated as a data-driven chance-constrained robust optimization problem. Effective red-light duration (ERD) is defined as a random variable, and describes the feasible passing time through the signalized intersections. Usually, the true probability distribution for ERD is unknown. Consequently, a data-driven approach is adopted to formulate chance constraints based on empirical sample data. This incorporates robustness into the eco-driving control problem with respect to uncertain signal timing. Dynamic programming (DP) is employed to solve the optimization problem. The simulation results demonstrate that the proposed method can generate optimal speed reference trajectories with 40% less vehicle fuel consumption, while maintaining the arrival time at a similar level compared to a modified intelligent driver model (IDM). The proposed control approach significantly improves the controller's robustness in the face of uncertain signal timing, without requiring to know the distribution of the random variable a priori.

33 ADVANCED PROPULSION SYSTEMS↗

Integrating Experiments and Well Logs to Predict Caney Shale Static Mechanical Properties During Production with Supervised Machine Learning

Caney shale is one of the emerging oil reservoirs in Oklahoma. Understanding the impact of effective stress on its mechanical properties is critical for predicting hydraulic fracture geometry and overall hydrocarbon production. The objective of our study is to evaluate the impact of effective stress on the dynamic Young’s modulus using ultrasonic velocity measurements for Caney shale samples. A triaxial cell was utilized to measure ultrasonic (P-wave and S-wave) velocities for ten downhole Caney shale samples under various effective stresses to indirectly assess the impact of pore pressure change. The dynamic Young’s moduli estimated from these measurements were integrated with available conventional well logs (excluding sonic logs) and triaxial test results from Benge et al. (2021) to predict the static Young’s modulus using Random Forest (RF) and Extreme Gradient Boosting (XGBoost) models. The results showed that the estimated dynamic Young’s moduli from ultrasonic measurements were higher than the corresponding static Young’s modulus of cores from the same vertical well at similar depths. With increasing effective stress, the dynamic Young’s modulus increased for all samples. The estimated dynamic-to-static correction factor tended to be higher in zones of high neutron porosity (PHIN) and low density compared to other zones. Finally, SHapley Additive exPlanations (SHAP) for RF and XGBoost models identified depth, gamma ray (GR), and PHIN as key features for predicting the static Young’s modulus. This study enhances our understanding of the dynamic and static Young’s moduli for the Caney shale interval, as a function of effective stress and conventional well logs. The findings from this study can improve predictions of production throughout the well's lifespan by offering insights into the mechanical property degradation resulting from pore pressure depletion.

Kholy, Sherif M.↗

Emission-line Galaxies from the Hubble Space Telescope Probing Evolution and Reionization Spectroscopically (Pears) Grism Survey. II. The Complete Sample

We present a full analysis of the Probing Evolution And Reionization Spectroscopically (PEARS) slitess grism spectroscopic data obtained with the Advanced Camera for Surveys on board Hubble Space Telescope. PEARS covers fields within both the Great Observatories Origins Deep Survey (GOODS) North and South fields, making it ideal as a random survey of galaxies, as well as the availability of a wide variety of ancillary observations complemented by the spectroscopic results. Using the PEARS data, we are able to identify star-forming galaxies (SFGs) within the redshift volume 0 < z < 1.5. Star-forming regions in the PEARS survey are pinpointed independently of the host galaxy. This method allows us to detect the presence of multiple emission-line regions (ELRs) within a single galaxy. We identified a total of 1162 Hα, [O III], and/or [O II] emission lines in the PEARS sample of 906 galaxies to a limiting flux of ~10 -18(exp) erg s -1(exp) cm -2(exp). The ELRs have also been compared to the properties of the host galaxy, including morphology, luminosity, and mass. From this analysis, we find three key results: (1) the computed line luminosities show evidence of a flattening in the luminosity function with increasing redshift; (2) the star-forming systems show evidence of complex morphologies with star formation occurring predominantly within one effective (half-light) radius. However, the morphologies show no correlation with host stellar mass. (3) Also, the number density of SFGs with M * ≥ 10 9(exp) M ☉ decreases by an order of magnitude at z ≤ 0.5 relative to the number at 0.5 < z < 0.9, supporting the argument of galaxy downsizing.

Hubble↗

Ensemble Federated Machine Learning‐Based Cybersecurity Situational Awareness in Microgrid Network

Cyber-physical microgrids are vulnerable to stealthy cybersecurity threats that disguise their actions through the exploitation of system knowledge. Such actions can severely impacts microgrids deployed in defense bases, slowing the response time of military forces during national emergencies. Several machine-learning algorithms have been proposed to detect intrusions in the grid networks; however, these traditional machine-learning algorithms lack data privacy and are subject to several adversarial machine-learning threats. This paper proposes a novel federated machine learning (FML)-based three-model framework to detect and identify stealthy data-integrity attacks while ensuring data privacy in microgrid networks. The proposed architecture uses a variational mode decomposition technique to extract derived features from incoming measurement and control datasets. The extraction of these derived features allows FML models to learn minute variations in data patterns that allow them to perform significantly better than the models trained with generic datasets consisting of raw features. Our experimental results show the efficient performance of the proposed methodology against different types of data integrity attacks while considering primary and secondary controllers in microgrids. Further, the applied FML-integrated random forest ensemble algorithm outperforms the existing generic FML algorithms during noisy and noise-free datasets with prediction latencies of only 91–134 µs per sample within the 0.1 s sampling interval and requires communication bandwidth of around ∼8.25 KB/s at the control center and ∼2.7 KB/s per edge client for communication.

24 POWER TRANSMISSION AND DISTRIBUTION↗

Comparison of venous and pooled capillary hemoglobin levels for the detection of anemia among adolescent girls

Introduction: Blood source is a known preanalytical factor affecting hemoglobin (Hb) concentrations, and there is evidence that capillary and venous blood may yield disparate Hb levels and anemia prevalence. However, data from adolescents are scarce. Objective: To compare Hb and anemia prevalence measured by venous and individual pooled capillary blood among a sample of girls aged 10–19 years from 232 schools in four regions of Ghana in 2022. Methods: Among girls who had venous blood draws, a random subsample was selected for capillary blood. Hb was measured using HemoCue® Hb-301. We used Lin’s concordance correlation coefficient (CCC) to quantify the strength of the bivariate relationship between venous and capillary Hb and a paired t-test for difference in means. We used McNemar’s test for discordance in anemia cases by blood source and weighted Kappa to quantify agreement by anemia severity. A multivariate generalized estimating equation was used to quantify adjusted population anemia prevalence and assess the association between blood source and predicted anemia risk. Results: We found strong concordance between Hb measures (CCC = 0.86). The difference between mean venous Hb (12.8 g/dL, ± 1.1) and capillary Hb (12.9 g/dL, ± 1.2) was not significant (p = 0.26). Crude anemia prevalence by venous and capillary blood was 20.6% and 19.5%, respectively. Adjusted population anemia prevalence was 23.5% for venous blood and 22.5% for capillary ( = 0.45). Blood source was not associated with predicted anemia risk (risk ratio: 0.99, 95% CI: 0.96, 1.02). Discordance in anemia cases by blood source was not significant (McNemar p = 0.46). Weighted Kappa demonstrated moderate agreement by severity (k =0.67). Among those with anemia by either blood source (n = 111), 59% were identified by both sources. Conclusion: In Ghanaian adolescent girls, there was no difference in mean Hb, anemia prevalence, or predicted anemia risk by blood source. However, only 59% of girls with anemia by either blood source were identified as having anemia by both sources. These findings suggest that pooled capillary blood may be useful for estimating Hb and anemia at the population level, but that caution is needed when interpreting individual-level data.

59 BASIC BIOLOGICAL SCIENCES↗

Interannual variability and predictability of 500 mb geopotential heights over the Northern Hemisphere

The interannual variability of monthly mean 500 mb heights in a 15-year sample of observed data is compared to the variance expected from sampling errors associated with high-frequency fluctuations using the analysis of variance approach. The monthly mean 'signal' stands out significantly from the 'noise' over a substantial fraction of the Northern Hemisphere during the winter. The expected spectrum of variance at very low frequencies is assumed to be white at frequencies lower than per (30 days) rather than the per (96 days) cutoff used by Madden. This difference is justified by observing that the effective time between independent samples T(0) is relatively insensitive to changes in the maximum lag over which the local autocorrelation is integrated to calculate T(0). Further non-randomness in the variance of 500 mb heights is evidenced by the correlation between monthly mean height and contemporaneous daily variability.

Shukla, J.↗

Longwave radiation parameterization for UCLA/GLAS GCM

This document describes the parameterization of longwave radiation in the UCLA/GLAS general circulation model. Transmittances have been computed from the work of Arking and Chou for water vapor and carbon dioxide and ozone absorptances are computed using a formula due to Rodgers. Cloudiness has been introduced into the code in a manner in which fractional cover and random or maximal overlap can be accommodated. The entire code has been written in a form that is amenable to vectorization on CYBER and CRAY computers. Sample clear sky computations for five standard profiles using the 15- and 9-level versions of the model have been included.

HARSHVARDHAN↗

Large N-body simulations of a universe dominated by cold dark matter

It is shown that pure gravitational evolution from an initial random density field with the standard cold dark matter (CDM) power spectrum does occasionally lead to filaments, walls, and voids remarkably like those seen in observed samples. A problem with the flat CDM model is the degree of streaming of galaxies relative to their velocity dispersion. A biased open CDM model seems to be more compatible with the observational data though it requires a higher amplitude of microwave background fluctuations.

Park, Changbom↗

Radar modeling of a boreal forest

Microwave modeling, ground truth, and SAR data are used to investigate the characteristics of forest stands. A mixed coniferous forest stand has been modeled at P, L, and C bands. Extensive measurements of ground truth and canopy geometry parameters were performed in a 200-m-square hemlock-dominated forest plot. About 10 percent of the trees were sampled to determine a distribution of diameter at breast height (DBH). Hemlock trees in the forest are modeled by characterizing tree trunks, branches, and needles as randomly oriented lossy dielectric cylinders whose area and orientation distributions are prescribed. The distorted Born approximation is used to compute the backscatter at P, L, and C bands. The theoretical results are found to be lower than the calibrated ground-truth data. The experiment and model results agree quite closely, however, when the ratios of VV to HH and HV to HH are compared.

Chauhan, Narinder S.↗

Environmental test program for superconducting materials and devices

A systematic approach to obtaining real time, superconducting YBa2Cu30(7-x) materials is presented. The work was carried out under the overall direction of Clemson University with tasks being performed at both Clemson and Westinghouse (Aiken, SC). Clemson prepared the tapecast superconducting 123 material and fabricated in into substrate-supported, environmentally-protected conducting links. Following this, all of the elements were individually tested for resistance vs. temperature and Tc; and then a portion of them were kept at Clemson for further testing while a randomly selected group was delivered to Westinghouse for specialized testing and evaluation in their low temperature/high vacuum and radiation facilities. In addition, a number of control samples (12 ea.) were put on the shelf at Clemson for further reference at the end of the testing period. The specific tests conducted at Clemson and Westinghouse/SRC are presented with a summary of the results.

Haertling, Gene↗