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At least 1,027 records · Page 57

Discrimination of Single Base Pair Differences Among Individual DNA Molecules Using a Nanopore

The protein toxin alpha-hemolysin form nanometer scale channels across lipid membranes. Our lab uses a single channel in an artificial lipid bilayer in a patch clamp device to capture and examine individual DNA molecules. This nanopore detector used with a support vector machine (SVM) can analyze DNA hairpin molecules on the millisecond time scale. We distinguish duplex stem length, base pair mismatches, loop length, and single base pair differences. The residual current fluxes also reveal structural molecular dynamics elements. DNA end-fraying (terminal base pair dissociation) can be observed as near full blockades, or spikes, in current. This technique can be used to investigate other biological processes dependent on DNA end-fraying, such as the processing of HIV DNA by HIV integrase.

Vercoutere, Wenonah↗

The identification of selected vegetation types in Arizona through the photointerpretation of intermediate scale aerial photography

The author has identified the following significant results. Nine photography interpretation tests were performed with a total of 19 different interpreters. Three tests were conducted with black and white intermediate scale photography and six tests with color infrared intermediate scale photography. The black and white test results show that the interpretation of vegetation mapped at the association level of classification is reliable for all the classes used at 61%. The color infrared tests indicate that the association level of mapping is unsatisfactory for vegetation interpretation of classes 1 and 6. Students' t-test indicated that intermediate scale black and white photography is significantly better than this particular color infrared photography for the interpretation of southeastern Arizona vegetation mapped at the association level.

Ross, G. F.↗

Response to Comment on "Does the Earth Have an Adaptive Infrared IRIS?"

Harrison's (2001) Comment on the Methodology in Lindzen et al (2001) has prompted re-examination of several aspects of study. Probably the most significant disagreement in our conclusions is due to our different approaches to minimizing the influence of long-time-scale variations in the variables A and T on the results. Given the strength of the annual cycle and the 20-month period covered by the data, we believe that removing monthly means is a better approach to minimizing the long-time-scale behavior of the data than removal of the linear trend, which might actually add spurious long- time- scale variability into the modified data. We have also indicated how our statistical methods of establishing statistical significance differ. More definitive conclusions may only possible after more data have been analyzed, but we feel that our results are robust enough to encourage further study of this phenomenon.

Bell, Thomas L.↗

Renewed Mapping of the Nepthys Mons Quadrangle (V-54), Venus

After a long hiatus due to competing tasks with the PI, mapping of Venus' Nepthys Mons Quadrangle (V-54, 300-330degE, 25-50degS) has been resumed, with planned submission late in 2008 or early 2009. Major goals are to determine the style of volcanism and tectonism over time, the evolution of shield volcanoes, the evolution of coronae, the characteristics of plains volcanism, and what these observations tell us about the general geologic history of Venus. This abstract largely repeats earlier progress reports, with some updates to show GEMS that the PI intends to complete this task in the near future. Methods: Geologic units and structures have been mapped onto hardcopy FMAPs and then transferred to the 1:5 million-scale map base (Figure 1). Pseudostereo anaglyphs have proved an indispensable tool and have resulted in a virtual complete revision of previously mapped areas [1,2]. At FMAP scale, structural trends and inferred ages are broken out using different symbols and colors. These are in the process of being transferred to a 1:5 million-scale structure map separate from the geologic map. The geologic units, structures, impact craters, coronae, and volcanoes are being arranged in time-stratigraphic sequences as the mapping progresses.

Bridges, Nathan T.↗

Reliable and Efficient Electrochemical Recovery of O 2 from Metabolic CO 2 at the International Space Station (ISS)

Maximum O 2 recovery from metabolic carbon dioxide (CO 2 ) is desired for future long-duration missions beyond Low Earth Orbit (LEO). The O 2 recovery for the Environmental Control and Life Support System (ECLSS) at the International Space Station (ISS), presently limited to 50% (Sabatier), must be highly reliable and efficient and recover a minimum of 75% oxygen (O 2 ) from metabolic CO 2 . An alternative technology development effort currently underway at NASA Marshall Space Flight Center (MSFC) via a Microfluidic Electrochemical Reactor (MFECR) approach has the potential to increase O 2 recovery significantly and reduce the complexity of the ECLSS O 2 recovery at the ISS as it would replace three pieces, the CO 2 Reduction Assembly (CRA) (Sabatier reactor), the Oxygen Generation Assembly (OGA), and the Plasma Pyrolysis Assembly (PPA). The MFECR's electrochemical process generates ethylene (C 2 H 4 ) and carbon moxide (CO) instead of methane (CH 4 ) (Sabatier) as a byproduct, eliminating the need for further dehydrogenation through the PPA. As in the OGA, the MFECR's electrochemical process generates O 2 and hydrogen (H 2 ) from the water electrolysis process. MSFC and the University of Texas in Arlington (UTA) have jointly designed and fabricated an MFECR's single cell that operates at ambient conditions and utilizes a proprietary catalysis highly selective on reducing CO 2 to C 2 H 4 and CO at the cathode. This MFECR's single cell consists of gas diffusion layers at the cathode and anode for respective intake of CO 2 and output of O 2 from the catalytic layer. This approach is expected to substantially improve the ISS ECLSS sustainability and reduce power and weight requirements as the MFECR would replace three units currently installed in the ISS. In this paper, the authors discuss the outcome of preliminary tests, the current development, and the evaluation efforts on different alternatives for the cathode and the anode configurations, the setup of the MFECR at an engineering development unit (EDU) scale, and the O 2 recovery performance, and evaluation efforts on different alternatives on not only the configuration and setup of the MFECR at an Engineering Design Unit (EDU) scale but also the selection of component materials.

Jesus A Dominguez↗

Approaches for averaging surface parameters and fluxes over heterogeneous terrain

Successful prediction of possible climate change depends on realistic parameterization of land surface processes in climate models. Such parameterizations must take appropriate account of the heterogeneities that are found in most earth surfaces. In this study, different averaging strategies for aggregating patch-scale heterogeneities to scales that are appropriate for mesoscale and climate model grids have been explored. A simple model for estimating area-average 'effective' surface flux parameters is evaluated. The model satisfies the energy balance equation and leads to a set of relationships between local and effective parameters in the governing equations for the surface energy balance. One outcome is that the resulting effective surface temperature is not a simple area-weighted average of component temperatures, but is a function of a specific combination of different resistances of the individual surface elements. A set of heterogeneous surfaces has been simulated to study the effective fluxes obtained using the described model. A comparison with results obtained by other investigators using different averaging methods is also performed.

Chehbouni, A.↗

Large-Scale Coronal Heating from "Cool" Activity in the Solar Magnetic Network

In Fe XII images from SOHO/EIT, the quiet solar corona shows structure on scales ranging from sub-supergranular (i.e., bright points and coronal network) to multi-supergranular (large-scale corona). In Falconer et al 1998 (Ap.J., 501, 386) we suppressed the large-scale background and found that the network-scale features are predominantly rooted in the magnetic network lanes at the boundaries of the supergranules. Taken together, the coronal network emission and bright point emission are only about 5% of the entire quiet solar coronal Fe XII emission. Here we investigate the relationship between the large-scale corona and the network as seen in three different EIT filters (He II, Fe IX-X, and Fe XII). Using the median-brightness contour, we divide the large-scale Fe XII corona into dim and bright halves, and find that the bright-half/dim half brightness ratio is about 1.5. We also find that the bright half relative to the dim half has 10 times greater total bright point Fe XII emission, 3 times greater Fe XII network emission, 2 times greater Fe IX-X network emission, 1.3 times greater He II network emission, and has 1.5 times more magnetic flux. Also, the cooler network (He II) radiates an order of magnitude more energy than the hotter coronal network (Fe IX-X, and Fe XII). From these results we infer that: 1) The heating of the network and the heating of the large-scale corona each increase roughly linearly with the underlying magnetic flux. 2) The production of network coronal bright points and heating of the coronal network each increase nonlinearly with the magnetic flux. 3) The heating of the large-scale corona is driven by widespread cooler network activity rather than by the exceptional network activity that produces the network coronal bright points and the coronal network. 4) The large-scale corona is heated by a nonthermal process since the driver of its heating is cooler than it is. This work was funded by the Solar Physics Branch of NASA's office of Space Science through the SR&T Program and the SEC Guest Investigator Program.

Falconer, D. A.↗

Fractal analysis of heart rate dynamics as a predictor of mortality in patients with depressed left ventricular function after acute myocardial infarction. TRACE Investigators. TRAndolapril Cardiac Evaluation

A number of new methods have been recently developed to quantify complex heart rate (HR) dynamics based on nonlinear and fractal analysis, but their value in risk stratification has not been evaluated. This study was designed to determine whether selected new dynamic analysis methods of HR variability predict mortality in patients with depressed left ventricular (LV) function after acute myocardial infarction (AMI). Traditional time- and frequency-domain HR variability indexes along with short-term fractal-like correlation properties of RR intervals (exponent alpha) and power-law scaling (exponent beta) were studied in 159 patients with depressed LV function (ejection fraction <35%) after an AMI. By the end of 4-year follow-up, 72 patients (45%) had died and 87 (55%) were still alive. Short-term scaling exponent alpha (1.07 +/- 0.26 vs 0.90 +/- 0.26, p <0.001) and power-law slope beta (-1.35 +/- 0.23 vs -1.44 +/- 0.25, p <0.05) differed between survivors and those who died, but none of the traditional HR variability measures differed between these groups. Among all analyzed variables, reduced scaling exponent alpha (<0.85) was the best univariable predictor of mortality (relative risk 3.17, 95% confidence interval 1.96 to 5.15, p <0.0001), with positive and negative predictive accuracies of 65% and 86%, respectively. In the multivariable Cox proportional hazards analysis, mortality was independently predicted by the reduced exponent alpha (p <0.001) after adjustment for several clinical variables and LV function. A short-term fractal-like scaling exponent was the most powerful HR variability index in predicting mortality in patients with depressed LV function. Reduction in fractal correlation properties implies more random short-term HR dynamics in patients with increased risk of death after AMI.

Non-NASA Center↗

Viscous Three-Dimensional Simulation of Flow in an Axial Low Pressure Compressor at Engine Icing Operating Points

Viscous three-dimensional simulations of the Honeywell ALF502R-5 low pressure compressor (sometimes called a booster) using the NASA Glenn code GlennHT have been carried out. A total of ten simulations were produced. Five operating points are investigated, with each point run with two different wall thermal conditions. These operating points are at, or near, points where engine icing has been determined to be likely. In the future, the results of this study will be used for further analysis such as predicting collection efficiency of ice particles and ice growth rates at various locations in the compressor. A mixing plane boundary condition is used between each blade row, resulting in convergence to steady state within each blade row. The k-omega turbulence model of Wilcox, combined with viscous grid spacing near the wall on the order of one, is used to resolve the turbulent boundary layers. For each of the operating points, heat transfer coefficients are generated on the blades and walls. The heat transfer coefficients are produced by running the operating point with two different wall thermal conditions and then solving simultaneously for the heat transfer coefficient and adiabatic wall temperature at each point. Average Nusselt numbers are calculated for the most relevant surfaces. The values are seen to scale with Reynolds number to approximately a power of 0.7. Additionally, images of surface distribution of Nusselt number are presented. Qualitative comparison between the five operating points show that there is relatively little change in the character of the distribution. The dominant observed effect is that of an overall scaling, which is expected due to Reynolds number differences. One interesting aspect about the Nusselt number distribution is observed on the casing (outer diameter) downstream of the exit guide vanes (EGVs). The Nusselt number is relatively high between the pairs of EGVs, with two lower troughs downstream of each EGV trailing edge. This is of particular interest since rather complex ice shapes have been observed in that region.

compressor↗

Seafloor Control on Sea Ice

The seafloor has a profound role in Arctic sea ice formation and seasonal evolution. Ocean bathymetry controls the distribution and mixing of warm and cold waters, which may originate from different sources, thereby dictating the pattern of sea ice on the ocean surface. Sea ice dynamics, forced by surface winds, are also guided by seafloor features in preferential directions. Here, satellite mapping of sea ice together with buoy measurements are used to reveal the bathymetric control on sea ice growth and dynamics. Bathymetric effects on sea ice formation are clearly observed in the conformation between sea ice patterns and bathymetric characteristics in the peripheral seas. Beyond local features, bathymetric control appears over extensive ice-prone regions across the Arctic Ocean. The large-scale conformation between bathymetry and patterns of different synoptic sea ice classes, including seasonal and perennial sea ice, is identified. An implication of the bathymetric influence is that the maximum extent of the total sea ice cover is relatively stable, as observed by scatterometer data in the decade of the 2000s, while the minimum ice extent has decreased drastically. Because of the geologic control, the sea ice cover can expand only as far as it reaches the seashore, the continental shelf break, or other pronounced bathymetric features in the peripheral seas. Since the seafloor does not change significantly for decades or centuries, sea ice patterns can be recurrent around certain bathymetric features, which, once identified, may help improve short-term forecast and seasonal outlook of the sea ice cover. Moreover, the seafloor can indirectly influence cloud cover by its control on sea ice distribution, which differentially modulates the latent heat flux through ice covered and open water areas.

Nghiem, S. V.↗

Dynamic localization and second-order subgrid-scale models in large eddy simulations of channel flow

The objective here is to test the Dynamic Localization (DL) model in a wall-bounded channel flow for numerical stability and accuracy of results. Algebraic stress models suggest that the model for the residual subgrid-scale (SGS) Reynolds stress and scalar flux should generally have terms comprising most of the unique products of the resolved strain (S) and rotation (R) tensors with S and the resolved scalar gradient. The standard dynamic SGS model uses a simple (Smagorinsky) base model for the residual Reynolds stress, which is made proportional to S, and down-gradient base models for residual scalar fluxes; these correspond to the lowest, 'first-order' terms in algebraic stress models. Temporal scaling terms in these base models are formed from the magnitude of the resolved strain rate. While this is appropriate for simple shear flows, it may not be appropriate for more complicated flows (relevant to geophysical and astrophysical problems) that include any combination of shear, rotation, buoyancy, etc. On the other hand, the coefficient in the dynamic SGS model readily adjusts itself to different flow conditions and may adequately take account of these effects without the need for more complicated base models. Cabot (1993) has begun to test the dynamic SGS model in buoyant flows (Rayleigh-Benard and internally heated convection) with and without buoyancy terms explicitly included in the scaling terms of the base model; no great differences were found in large eddy simulation (LES) results for the different base model scalings. The second objective in this work is to test base models with additional, 'second-order' terms (e.g., S(sup 2) and RS for the residual Reynolds stress). These terms have been found to improve large-scale flow predictions by kappa-epsilon models in the presence of rotation and shear. Second-order base models will be tested here in the LES of channel flow with and without solid-body rotation and compared with results from the standard first-order base models to determine if there are significant differences or improvements in results that would warrant the added complexity of the second-order base models.

Cabot, William H.↗

Atmospheric planetary wave response to external forcing

The tools of observational analysis, complex general circulation modeling, and simpler modeling approaches were combined in order to attack problems on the largest spatial scales of the earth's atmosphere. Two different models were developed and applied. The first is a two level, global spectral model which was designed primarily to test the effects of north-south sea surface temperature anomaly (SSTA) gradients between the equatorial and midlatitude north Pacific. The model is nonlinear, contains both radiation and a moisture budget with associated precipitation and surface evaporation, and utilizes a linear balance dynamical framework. Supporting observational analysis of atmospheric planetary waves is briefly summarized. More extensive general circulation models have also been used to consider the problem of the atmosphere's response, especially in the horizontal propagation of planetary scale waves, to SSTA.

Stevens, D. E.↗

Sporadic narrowband radio emissions from Uranus

Among several different types of radio emissions discovered at Uranus during the Voyager 2 encounter in January 1986 is a very sporadic, bursty signal which consists of very narrow bands lying in the frequency range from about 3 to 10 kHz. The bursty emission was virtually undetectable from the dayside portion of the Voyager 2 trajectory, but was observed out to beyond 300 R(U) during the outbound trajectory through the predawn sector. While the narrowband tones making up this emission are reminiscent of escaping continuum radiation observed near earth, Jupiter, and Saturn, the Uranian signals show large amplitude variations on time scales of 1 s, suggesting a very different type of generation mechanism.

Kurth, W. S.↗

Radio studies of large scale structures of the Sun's corona and transient activity

The use of meter-decimeter wavelength imaging observations for four different kinds of studies of solar coronal activity is discussed. (1) Large scale structure of the upper corona; daily imaging observations permit comparison of radio images with white light images from space and ground observation, generation of synoptic charts similar to white light coronagraph synoptic charts, and comparison of radio brightness enhancements and deficiencies with bright coronal streamers and coronal holes. (2) Relative positions of type III burst sources and coronal streamers as observed by Solwind experiment on the P-78-1 satellite and by the HAO C/P experiment aboard the SMM; infer the paths of type III emitting electrons in dense coronal streamers, and from multifrequency observations derive electron density distributions above active regions near the limb. (3) Non-flare associated type II/type IV bursts associated with coronal streamer disruption events; such type IV sources have a rather slow velocity (approx = to or less than 100 km/s) CMEs. (4) Meter-decimeter microbursts; these are short duration (2-10 sec) weak-type III-like bursts, produced at the fundamental plasma frequency by plasma radiation processes which have important differences from the standard mechanisms used to explain the strong type III bursts.

Kundu, Mukul R.↗

Atmospheric flow over two-dimensional bluff surface obstructions

The phenomenon of atmospheric flow over a two-dimensional surface obstruction, such as a building (modeled as a rectangular block, a fence or a forward-facing step), is analyzed by three methods: (1) an inviscid free streamline approach, (2) a turbulent boundary layer approach using an eddy viscosity turbulence model and a horizontal pressure gradient determined by the inviscid model, and (3) an approach using the full Navier-Stokes equations with three turbulence models; i.e., an eddy viscosity model, a turbulence kinetic-energy model and a two-equation model with an additional transport equation for the turbulence length scale. A comparison of the performance of the different turbulence models is given, indicating that only the two-equation model adequately accounts for the convective character of turbulence. Turbulence flow property predictions obtained from the turbulence kinetic-energy model with prescribed length scale are only insignificantly better than those obtained from the eddy viscosity model. A parametric study includes the effects of the variation of the characteristics parameters of the assumed logarithmic approach velocity profile. For the case of the forward-facing step, it is shown that in the downstream flow region an increase of the surface roughness gives rise to higher turbulence levels in the shear layer originating from the step corner.

Bitte, J.↗

The Effects of Reynolds Number on the Application of NACA 16 Series Airfoil Characteristics to Propeller Design

An analysis has been made of airfoil data taken on several NACA 16-series propeller airfoils from tests of 5-inch-chord models in the Langley 24 inch high-speed tunnel and l2-inch-chord models in the Langley 8 foot high-speed tunnel, This analysis has shown that the combined effects of Reynolds number changes and variations in airfoil characteristics resulting from differences in models and tunnels are such that when 5 inch-chord and l2-inch-chord data are applied to full-scale propeller design at or near the design condition, differences of less than 1 percent in efficiency will be involved.

Cleary, Harold E.↗

Noise of the Harrier in vertical landing and takeoff

The noise of the Harrier AV8C aircraft in vertical takeoff and landing was measured 100 feet to the side of the aircraft where jet noise dominates. The noise levels were quite high - up to 125 dB overall sound level at 100 feet. The increased noise due to jet impingement on the ground is presented as a function of jet height to diameter ratio. The impingement noise with the aircraft close to the ground was 14 to 17 dB greater than noise from a free jet. Results are compared with small-scale jet impingement data acquired elsewhere. The agreement between small-scale and full-scale noise increase in ground effect is fairly good except with the jet close to the ground. It is proposed that differences in the jet Reynolds numbers and the resultant character of the jets may be partially responsible for the disparity in the full-scale and small-scale jet impingement noise. The difference between single-jet impingement and multiple-jet impingement may also have been responsible for the small-scale and full-scale disagreement.

Soderman, Paul T.↗

A dynamic subgrid-scale eddy viscosity model

One major drawback of the eddy viscosity subgrid-scale stress models used in large-eddy simulations is their inability to represent correctly with a single universal constant different turbulent field in rotating or sheared flows, near solid walls, or in transitional regimes. In the present work, a new eddy viscosity model is presented which alleviates many of these drawbacks. The model coefficient is computed dynamically as the calculation progresses rather than input a priori. The model is based on an algebraic identity (Germano 1990) between the subgrid-scale stresses at two different filtered levels and the resolved turbulent stresses. The subgrid-scale stresses obtained using the proposed model vanish in laminar flow and at a solid boundary, and have the correct asymptotic behavior in the near-wall region of a turbulent boundary layer. The results of large-eddy simulations of transitional and turbulent channel flow that use the proposed model are in good agreement with the direct simulation data.

Germano, Massimo↗