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At least 1,027 records · Page 57
All sourcesNASA NTRSDOE OSTI
NASA NTRS2002

Tetracycline rapidly reaches all the constituent cells of uropathogenic Escherichia coli biofilms

We have developed a method for visualizing Escherichia coli cells that are exposed to tetracycline in a biofilm, based on a previous report that liposomes containing the E. coli TetR(B) protein fluoresce when exposed to this antibiotic. By our method, cells devoid of TetR(B) also exhibited tetracycline-dependent fluorescence. At 50 microg of tetracycline ml(-1), planktonic cells of a uropathogenic E. coli (UPEC) strain developed maximal fluorescence after 7.5 to 10 min of exposure. A similar behavior was exhibited by cells in a 24- or 48-h UPEC biofilm, as examined by confocal laser microscopy, regardless of whether they lined empty spaces or occupied densely packed regions. Further, a comparison of phase-contrast and fluorescent images of corresponding biofilm zones showed that all the cells fluoresced. Thus, all the biofilm cells were exposed to tetracycline and there were no pockets within the biofilm where the antibiotic failed to reach. It also appeared unlikely that niches of reduced exposure to the antibiotic existed within the biofilms.

Non-NASA Center↗
NASA NTRS1996

Structural relations between collagen and mineral in bone as determined by high voltage electron microscopic tomography

Aspects of the ultrastructural interaction between collagen and mineral crystals in embryonic chick bone have been examined by the novel technique of high voltage electron microscopic tomography to obtain three-dimensional information concerning extracellular calcification in this tissue. Newly mineralizing osteoid along periosteal surfaces of mid-diaphyseal regions from normal chick tibiae was embedded, cut into 0.25 microns thick sections, and documented at 1.0 MV in the Albany AEI-EM7 high voltage electron microscope. The areas of the tissue studied contained electron dense mineral crystals associated with collagen fibrils, some marked by crystals disposed along their cylindrically shaped lengths. Tomographic reconstructions of one site with two mineralizing fibrils were computed from a 5 degrees tilt series of micrographs over a +/- 60 degrees range. Reconstructions showed that the mineral crystals were platelets of irregular shape. Their sizes were variable, measured here up to 80 x 30 x 8 nm in length, width, and thickness, respectively. The longest crystal dimension, corresponding to the c-axis crystallographically, was generally parallel to the collagen fibril long axis. Individual crystals were oriented parallel to one another in each fibril examined. They were also parallel in the neighboring but apparently spatially separate fibrils. Crystals were periodically (approximately 67 nm repeat distance) arranged along the fibrils and their location appeared to correspond to collagen hole and overlap zones defined by geometrical imaging techniques. The crystals appeared to be continuously distributed along a fibril, their size and number increasing in a tapered fashion from a relatively narrow tip containing smaller and infrequent crystals to wider regions having more densely packed and larger crystals. Defined for the first time by direct visual 3D imaging, these data describe the size, shape, location, orientation, and development of early crystals in normal bone collagen. The results suggest that platelet-shaped crystals are arranged in channels or grooves which are formed by collagen hole zones in register and that crystal sizes may exceed the dimensions of hole zones. Such data agree with those from mineral-matrix interaction in normally calcifying avian tendon obtained by similar high voltage tomographic means, but in addition they indicate a possible gradual and continuous deposition of crystals in collagen of bone unlike tendon and imply that individual collagen fibrils in local regions of osteoid are organized such that they all may be aligned in a coherent manner.

NASA Discipline Number 40-40↗
NASA NTRSDate not supplied

Neuro-Vestibular Examination During and Following Spaceflight (Vestibular Health)

BACKGROUND Adaptation to microgravity during spaceflight causes neurological disturbances that are either directly or indirectly mediated by the vestibular system. These disturbances could include space motion sickness, spatial disorientation, and cognitive impairment, as well as changes in head-eye coordination, vestibulo-ocular reflexes, and control of posture and locomotion. Otolith-mediated reflex gains appear to adapt rapidly during spaceflight and after landing. However, animal studies have shown that structural modifications of the vestibular sensory apparatus develop during long-duration spaceflight. To date, no studies have characterized the severity of vestibular syndromes experienced by astronauts as a function of the duration of spaceflight or whether the effects are caused by changes at the peripheral end organs, midbrain, cerebellum, or vestibular cortex. OBJECTIVES We will investigate temporal vestibular changes in crewmembers of short, 6-month, and one-year missions to identify trends in adaptation of vestibular health and performance in orbit and after landing. We will also determine whether the vestibular organs and/or the central vestibular system undergo structural changes during long-duration exposure to microgravity, which could cause vestibular disorders when transitioning to a different gravitational environment. METHODS Recordings of eye, head, and body movements, as well as subjective reports of perception of motion, will be used to determine the presence of abnormal eye movements, dysmetria, motion sickness symptoms, and illusions of motion during head or body movements. This includes characterization of temporal trends in central compensation for vestibular (otolith) asymmetry. Pre-flight data will be collected 90 days before the flight. In-flight tests will be performed early in the mission and once every one or two months thereafter. Post-flight examinations will be performed on the following days after return (R) from the mission: R+0, R+4, R+9, and R+30. Ground-based control tests will be performed on healthy volunteers in the laboratory to estimate mean normative responses. CONTROL RESULTS Thirty-two healthy (non-astronaut) control subjects performed the same ground test procedures as planned for crewmembers. In addition to establishing a normative database, these data were used to calculate vestibular asymmetries from perceptual reports during unilateral centrifugation, oculomotor responses during visual-alignment tasks, vestibulo-ocular reflex gain during head-impulse tests, and body rotation during stepping tests. A significant correlation was observed between asymmetries of subjective visual vertical and verbal report during unilateral centrifugation. Another significant correlation was observed between the asymmetries of ocular alignment, vestibulo-ocular reflex gain, and body rotation. These findings in a healthy cohort may help us better understand changes in vestibular asymmetries in crewmembers during and following spaceflight. RELEVANCE If the observed symptoms in crewmembers are more deleterious after the year-long missions than those documented after 6-month missions, then relevant countermeasures will be required to maintain the health and operational performance of astronauts during longer missions. Depending on the etiology of the vestibular syndrome revealed by these tests, countermeasures will be proposed based on vestibular rehabilitation therapies currently used in patients with vestibular disorders, such as habituation, gaze stabilization, and/or balance training exercises. ACKNOWLEDGEMENT This work is supported by the NASA’s Human Research Program Human Health Countermeasures Element.

T R Macaulay↗
DOE OSTI2023

Domain Adaptive Graph Neural Networks for Constraining Cosmological Parameters Across Multiple Data Sets

Deep learning models have been shown to outperform methods that rely on summary statistics, like the power spectrum, in extracting information from complex cosmological data sets. However, due to differences in the subgrid physics implementation and numerical approximations across different simulation suites, models trained on data from one cosmological simulation show a drop in performance when tested on another. Similarly, models trained on any of the simulations would also likely experience a drop in performance when applied to observational data. Training on data from two different suites of the CAMELS hydrodynamic cosmological simulations, we examine the generalization capabilities of Domain Adaptive Graph Neural Networks (DA-GNNs). By utilizing GNNs, we capitalize on their capacity to capture structured scale-free cosmological information from galaxy distributions. Moreover, by including unsupervised domain adaptation via Maximum Mean Discrepancy (MMD), we enable our models to extract domain-invariant features. We demonstrate that DA-GNN achieves higher accuracy and robustness on cross-dataset tasks (up to $28\%$ better relative error and up to almost an order of magnitude better $\chi^2$). Using data visualizations, we show the effects of domain adaptation on proper latent space data alignment. This shows that DA-GNNs are a promising method for extracting domain-independent cosmological information, a vital step toward robust deep learning for real cosmic survey data.

79 ASTRONOMY AND ASTROPHYSICS↗
NASA NTRS1990

Blue ellipticals in compact groups

By studying galaxies in compact groups, the authors examine the hypothesis that mergers of spiral galaxies make elliptical galaxies. The authors combine dynamical models of the merger-rich compact group environment with stellar evolution models and predict that roughly 15 percent of compact group ellipticals should be 0.15 mag bluer in B - R color than normal ellipticals. The published colors of these galaxies suggest the existence of this predicted blue population, but a normal distribution with large random errors can not be ruled out based on these data alone. However, the authors have new ultraviolet blue visual data which confirm the blue color of the two ellipticals with blue B - R colors for which they have their own colors. This confirmation of a population of blue ellipticals indicates that interactions are occurring in compact groups, but a blue color in one index alone does not require that these ellipticals are recent products of the merger of two spirals. The authors demonstrate how optical spectroscopy in the blue may distinguish between a true spiral + spiral merger and the swallowing of a gas-rich system by an already formed elliptical. The authors also show that the sum of the luminosity of the galaxies in each group is consistent with the hypothesis that the final stage in the evolution of compact group is an elliptical galaxy.

Zepf, Stephen E.↗
NASA NTRS1994

Implementation of and measurement with the LIPA technique in a subsonic jet

LIPA (Laser Induced Photochemical Anemometry) was used to measure velocity, vorticity, Reynolds stress, and turbulent intensity distributions in a subsonic jet. The jet region of interest was the area close to the jet-orifice. The LIPA-technique is a nonintrusive quantitative flow visualization technique, consisting of tracking a phosphorescing grid of fluid particles, which is impressed by laser-beams directed into the flow. The phosphorescence of biacetyl gas was used to enable tracking of the impressed light grid. In order to perform measurements in a jet, LIPA was developed and implemented for the specific flow requirements. Nitrogen was used as the carrier gas to avoid quenching of the phosphorescent radiation of the tracer gas biacetyl by ambient oxygen. The use of sulfur dioxide to sensitize phosphorescent emission of biacetyl was examined. Preliminary data was used in a discussion of the potential of the LIPA technique.

Falco, R. E.↗
NASA NTRS2007

Effects of Artificial Gravity and Bed Rest on Spatial Orientation and Balance Control

While the vestibular system should be well-adapted to bed rest (a condition it experiences approximately 8/24 hrs each day), questions remain regarding the degree to which repeated exposures to the unusual gravito-inertial force environment of a short-radius centrifuge might affect central processing of vestibular information used in spatial orientation and balance control. Should these functions be impaired by intermittent AG, its feasibility as a counter-measure would be diminished. We, therefore, examined the effects of AG on spatial orientation and balance control in 15 male volunteers before and after 21 days of 6 HDT bed rest (BR). Eight of the subjects were treated with daily 1hr AG exposures (2.5g at the feet; 1.0g at the heart) aboard a short radius (3m) centrifuge, while the other seven served as controls (C). Spatial orientation was assessed by measures of ocular counter-rolling (OCR; rotation of the eye about the line of sight, an otolith-mediated reflex) and subjective visual vertical (SVV; perception of the spatial upright). Both OCR and SVV measurements were made with the subject upright, lying on their left sides, and lying on their right sides. OCR was measured from binocular eye orientation recordings made while the subjects fixated for 10s on a point target directly in front of the face at a distance of 1 m. SVV was assessed by asking subjects (in the dark) to adjust to upright (using a handheld controller) the orientation of a luminous bar randomly perturbed (15) to either side of the vertical meridian. Balance control performance was assessed using a computerized dynamic posturography (CDP) protocol similar to that currently required for all returning crew members. During each session, the subjects completed a combination of trials of sensory organization test (SOT) 2 (eyes closed, fixed platform) and SOT 5 (eyes closed, sway-referenced platform) with and without static and dynamic pitch plane head movements (plus or minus 20 deg., dynamic paced by an audible tone at 0.33Hz). OCR and CDP performance were unaffected by BR and BR+AG; post-BR measures were unchanged from baseline for both AG and C groups. Similarly, BR did not affect SVV in the C group. However, BR+AG disrupted one measure of spatial orientation: SVV error was significantly increased on R+0 and R+1 following BR in the AG group. These results suggest a transient untoward effect on central vestibular processing may accompany repeated exposure to intermittent AG, a potential side-effect that should be studied more closely in future studies.

Paloski, William H.↗
NASA NTRS2016

Crew Exploration Vehicle (CEV) (Orion) Occupant Protection

The purpose of this study was to determine the similarity between the response of the THUMS model and the Hybrid III Anthropometric Test Device (ATD) given existing Wright-Patterson (WP) sled tests. There were four tests selected for this comparison with frontal, spinal, rear, and lateral loading. The THUMS was placed in a sled configuration that replicated the WP configuration and the recorded seat acceleration for each test was applied to model seat. Once the modeling simulations were complete, they were compared to the WP results using two methods. The first was a visual inspection of the sled test videos compared to the THUMS d3plot files. This comparison resulted in an assessment of the overall kinematics of the two results. The other comparison was a comparison of the plotted data recorded for both tests. The metrics selected for comparison were seat acceleration, belt forces, head acceleration and chest acceleration. These metrics were recorded in all WP tests and were outputs of the THUMS model. Once the comparison of the THUMS to the WP tests was complete, the THUMS model output was also examined for possible injuries in these scenarios. These outputs included metrics for injury risk to the head, neck, thorax, lumbar spine and lower extremities. The metrics to evaluate head response were peak head acceleration, HIC15, and HIC36. For the neck, N (sub ij) was calculated. The thorax response was evaluated with peak chest acceleration, the Combined Thoracic Index (CTI), sternal deflection, chest deflection, and chest acceleration- 3 ms clip. The lumbar spine response was evaluated with lumbar spine force. Finally the lower extremity response was evaluated by femur and tibia force. The results of the simulation comparisons indicate the THUMS model had a similar response to the Hybrid III dummy given the same input. The primary difference seen between the two was a more flexible response of the THUMS compared to the Hybrid III. This flexibility was most pronounced in the neck flexion, shoulder deflection and chest deflection. Due to the flexibility of the THUMS, the resulting head and chest accelerations tended to lag the Hybrid III acceleration trace and have a lower peak value. The results of the injury metric comparison identified possible injury trends between simulations. Risk of head injury was highest for the lateral simulations. The risk of chest injury was highest for the rear impact. However, neck injury risk was approximately the same for all simulations. The injury metric value for lumbar spine force was highest for the spinal impact. The leg forces were highest for the rear and lateral impacts. The results of this comparison indicate the THUMS model performs in a similar manner as the Hybrid III ATD. The differences in the responses of model and the ATD are primarily due to the flexibility of the THUMS. This flexibility of the THUMS would be a more human like response. Based on the similarity between the two models, the THUMS should be used in further testing to assess risk of injury to the occupant.

Currie-Gregg, Nancy J.↗
DOE OSTI2022

Star-forming S0 Galaxies in SDSS-MaNGA: fading spirals or rejuvenated S0s?

ABSTRACT We investigate the origin of rare star formation in an otherwise red-and-dead population of S0 galaxies, using spatially resolved spectroscopy. Our sample consists of 120 low redshift (z < 0.1) star-forming S0 (SF-S0) galaxies from the SDSS-IV MaNGA DR15. We have selected this sample after a visual inspection of deep images from the DESI Legacy Imaging Surveys DR9 and the Subaru/HSC-SSP survey PDR3 to remove contamination from spiral galaxies. We also construct two control samples of star-forming spirals (SF-Sps) and quenched S0s (Q-S0s) to explore their evolutionary link with the star-forming S0s. To study star formation at resolved scales, we use dust-corrected H α luminosity and stellar density (Σ⋆) maps to construct radial profiles of star formation rate (SFR) surface density (ΣSFR) and specific SFR (sSFR). Examining these radial profiles, we find that star formation in SF-S0s is centrally dominated as opposed to disc-dominated star formation in spirals. We also compared various global (size–mass relation, bulge-to-total luminosity ratio) and local (central stellar velocity dispersion) properties of SF-S0s to those of the control sample galaxies. We find that SF-S0s are structurally similar to the quenched S0s and are different from star-forming spirals. We infer that SF-S0s are unlikely to be fading spirals. Inspecting stellar and gas velocity maps, we find that more than $50{{\ \rm per\ cent}}$ of the SF-S0 sample shows signs of recent galaxy interactions such as kinematic misalignment, counter-rotation, and unsettled kinematics. Based on these results, we conclude that in our sample of SF-S0s, star formation has been rejuvenated, with minor mergers likely to be a major driver.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2020

75 Years of Drift: The changing meaning of phasor [History]

The origin of what electrical engineers call a phasor diagram is explored, and traced to a treatise by William Thomson (later Lord Kelvin) and Peter Tait. The diagram was mechanical in origin, and gave a geometrical way to visualize and calculate the interactions of sinusoidal motions. Originally, the words radius vector were used in association with the diagram. Later authors used vector. As work on electromagnetic fields expanded, the word phasor was introduced to replace it. That change came about as a result of a deliberate effort (in 1944) to have a clear and unambiguous term of art. Since then, the meaning of the word has changed, and the geometrical interpretation has fallen out of favor. Unfortunately, clarity and unambiguity have suffered as a result. Newer meanings are examined. The measurement of phase angle in the electric power system is described. An Appendix on the work of Steinmetz shows that he did not favor the phasor representation.

Vector, Phasor, Phasor Measurement Unit↗
NASA NTRS1989

Modeling and sensory feedback control for space manipulators

The positioning control problem of the endtip of space manipulators whose base are uncontrolled is examined. In such a case, the conventional control method for industrial robots based on a local feedback at each joint is not applicable, because a solution of the joint displacements that satisfies a given position and orientation of the endtip is not decided uniquely. A sensory feedback control scheme for space manipulators based on an artificial potential defined in a task-oriented coordinates is proposed. Using this scheme, the controller can easily determine the input torque of each joint from the data of an external sensor such as a visual device. Since the external sensor is mounted on the unfixed base, the manipulator must track the moving image of the target in sensor coordinates. Moreover the dynamics of the base and the manipulator are interactive. However, the endtip is proven to asymptotically approach the stationary target in an inertial coordinate frame by the Liapunov's method. Finally results of computer simulation for a 6-link space manipulator model show the effectiveness of the proposed scheme.

Masutani, Yasuhiro↗
NASA NTRS2004

Magnetosphere-Ionosphere Coupling in the Auroral Zone

The visual light display at high latitudes referred to as the aurora fascinates casual observers and researchers alike. The natural question is what causes the aurora? We know that energized electrons streaming along the Earth's ambient magnetic field and colliding with atmospheric particles produce aurora. We do not know for certain, however, how these electrons are accelerated to high energies primarily in the field-aligned direction toward the Earth, or what the drivers of this acceleration are. As such, the goal of this Guest Investigator research project was to examine the physical processes that can cause field-aligned acceleration of plasma particles in the auroral region.

Schriver, David↗
NASA NTRS2016

Preliminary Results From a Heavily Instrumented Engine Ice Crystal Icing Test in a Ground Based Altitude Test Facility

Preliminary results from the Heavily Instrumented ALF503R-5 Engine test conducted in the NASA Glenn Research Center Propulsion Systems Laboratory will be discussed. The effects of ice crystal icing on a full scale engine is examined and documented. This model engine, serial number LF01, was used during the inaugural icing test in the PSL facility. The reduction of thrust (rollback) events experienced by this engine in flight were replicated in the facility. Limited instrumentation was used to detect icing. Metal temperature on the exit guide vanes and outer shroud and the load measurement were the only indicators of ice formation. The current study features a similar engine, serial number LF11, which is instrumented to characterize the cloud entering the engine, detect characterize ice accretion, and visualize the ice accretion in the region of interest.

enigine icing↗
NASA NTRS1993

Three-dimensional Structure of Straight and Curved Plane Wakes

Although the plane wake is marked by the formation of strong spanwise vortices, the initially two-dimensional Karman-like vortices soon develop a three-dimensional structure in the form of secondary streamwise vortices. So far, this streamwise vortex structure has been studied mostly through flow visualization and at relatively low Reynolds numbers. The primary objective of the present program was to investigate the origin and evolution of the three-dimensional structure of straight and curved plane wakes at relatively high Reynolds numbers (Re(sub b) = 28,000) through detailed measurements of the mean and turbulent properties at several streamwise locations. The experiments were conducted in three phases. In the first phase, the development of a straight plane wake was investigated. In the second phase, the effects of imposed streamwise curvature on the wake development were examined. The streamwise curvature was of constant radius and very mild in terms of the curvature ratio (b/(square root of R) is less than 2 percent). In both the first and second phases, the role of initial conditions was examined in wakes generated from both untripped (laminar) and tripped (turbulent) initial boundany layers. In the third phase, the effects of injecting streamwise vorticity and the effects of increased Reynolds number on the tripped wake structure and development were investigated.

Weygandt, James H.↗
NASA NTRS2015

Fluid Shifts

INTRODUCTION: Mechanisms responsible for the ocular structural and functional changes that characterize the visual impairment and intracranial pressure (ICP) syndrome (VIIP) are unclear, but hypothesized to be secondary to the cephalad fluid shift experienced in spaceflight. This study will relate the fluid distribution and compartmentalization associated with long-duration spaceflight with VIIP symptoms. We also seek to determine whether the magnitude of fluid shifts during spaceflight, as well as the VIIP-related effects of those shifts, can be predicted preflight with acute hemodynamic manipulations, and also if lower body negative pressure (LBNP) can reverse the VIIP effects. METHODS: Physiologic variables will be examined pre-, in- and post-flight in 10 International Space Station crewmembers including: fluid compartmentalization (D2O and NaBr dilution); interstitial tissue thickness (ultrasound); vascular dimensions and dynamics (ultrasound and MRI (including cerebrospinal fluid pulsatility)); ocular measures (optical coherence tomography, intraocular pressure, ultrasound); and ICP measures (tympanic membrane displacement, otoacoustic emissions). Pre- and post-flight measures will be assessed while upright, supine and during 15 deg head-down tilt (HDT). In-flight measures will occur early and late during 6 or 12 month missions. LBNP will be evaluated as a countermeasure during HDT and during spaceflight. RESULTS: The first two crewmembers are in the preflight testing phase. Preliminary results characterize the acute fluid shifts experienced from upright, to supine and HDT postures (increased stroke volume, jugular dimensions and measures of ICP) which are reversed with 25 millimeters Hg LBNP. DISCUSSION: Initial results indicate that acute cephalad fluid shifts may be related to VIIP symptoms, but also may be reversible by LBNP. The effect of a chronic fluid shift has yet to be evaluated. Learning Objectives: Current spaceflight VIIP research is described, including novel hardware and countermeasures.

Stenger, M. B.↗
DOE OSTI2020

Statistical properties of homogeneous and isotropic turbulence in He II measured via particle tracking velocimetry

Despite being a quantum two-fluid system, superfluid helium-4 (He II) is observed to behave similarly to classical fluids when a flow is generated by mechanical forcing. This similarity has brought up the feasibility of utilizing He II for high Reynolds number classical turbulence research, considering the small kinematic viscosity of He II. However, it has been suggested that the nonclassical dissipation mechanism in He II at small scales may alter its turbulent statistics and intermittency. In this work, we report our study of a nearly homogeneous and isotropic turbulence (HIT) generated by a towed grid in He II. We measure the velocity field using particle tracking velocimetry with solidified deuterium particles as the tracers. By correlating the velocities measured simultaneously on different particle trajectories or at different times along the same particle trajectory, we are able to conduct both Eulerian and Lagrangian flow analyses. Spatial velocity structure functions obtained through the Eulerian analysis show scaling behaviors in the inertial subrange similar to that for classical HIT but with enhanced intermittency. The Lagrangian analysis allows us to examine the flow statistics down to below the dissipation length scale. Interestingly, abnormal deviations from the classical scaling behaviors are observed in this regime. Lastly, we discuss how these deviations may relate to the motion of quantized vortices in the superfluid component in He II.

75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND↗
DOE OSTI2026

Advanced Research on Integrated Energy Systems Cyber Range

As digital technologies expand to meet the needs of a more autonomous, interconnected, and advanced power system, new cybersecurity complexities and vulnerabilities arise. The ARIES Cyber Range enables the energy sector to evaluate these evolutions and validate cybersecurity solutions without impacting live systems. Combining power grid-scale hardware with emulation and simulation approaches, the ARIES Cyber Range can faithfully replicate modern energy systems - from grid physics to communication networks, and everything in between - with real-world fidelity. At NLR, researchers and partners are answering complex power system cybersecurity questions, examining emerging threats to the electric sector, and de risking new security technologies, all at a mission-relevant speed that keeps pace with rapidly evolving systems and hazards.

29 ENERGY PLANNING, POLICY, AND ECONOMY↗
DOE OSTI2026

Ten questions concerning low-cost indoor air quality sensors: Perspectives from research and practice

Low-cost indoor air quality (IAQ) sensors are increasingly being used in homes and commercial and public buildings, driven by growing concerns about the impact of air on health, cognitive performance, and occupant wellbeing. These sensors offer a potentially transformative opportunity to increase spatial and temporal coverage of IAQ monitoring at a fraction of the cost of conventional reference instruments. However, their widespread use raises questions around accuracy, calibration, placement, data handling and interpretation, and integration into existing standards and workflows. This paper presents ten critical questions concerning the use of low-cost IAQ sensors in buildings, drawing on the latest empirical research, field deployments, and emerging practice. It discusses potential frameworks for deployment and evaluation, examines current sensor capabilities for measuring common pollutants, identifies methodological gaps in validation and uncertainty quantification, and outlines the extent to which existing IAQ standards can accommodate sensor-based evidence. The paper also explores how monitoring needs and deployment models vary by building type, the potential of real-time IAQ data to support building operations, and the ethical and legal implications of widespread sensor use. While significant challenges remain in ensuring data quality and building stakeholder trust, new applications are emerging through open data initiatives and advances in analytics and visualization. As the technology, science, and standards co-evolve, low-cost IAQ sensors are poised to become integral to routine building operation, building science, and environmental health research.

Parkinson, Thomas↗