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Entry, Descent, and Landing technological barriers and crewed MARSvehicle performance analysis

Mars has been explored historically only by robotic crafts, but a crewed mission encompasses several new engineering challenges – high ballistic coefficient entry, hypersonic decelerators, guided entry for reaching intended destinations within acceptable margins for error in the landing ellipse, and payload mass are all critical factors for evaluation. A comprehensive EDL parametric analysis has been conducted in support of a high mass landing architecture by evaluating three types of vehicles −70° Sphere Cone, Ellipsled and SpaceX hybrid architecture called Red Dragon as potential candidate options for crewed entry vehicles. Aerocapture at the Martian orbit of about 400 km and subsequent Entry-from-orbit scenarios were investigated at velocities of 6.75 km/s and 4 km/s respectively. A study on aerocapture corridor over a range of entry velocities (6–9 km/s) suggests that a hypersonic L/D of 0.3 is sufficient for a Martian aerocapture. Parametric studies conducted by varying aeroshell diameters from 10 m to 15 m for several entry masses up to 150 mt are summarized and results reveal that vehicles with entry masses in the range of about 40–80 mt are capable of delivering cargo with a mass on the order of 5–20 mt. For vehicles with an entry mass of 20 mt to 80 mt, probabilistic Monte Carlo analysis of 5000 cases for each vehicle were run to determine the final landing ellipse and to quantify the statistical uncertainties associated with the trajectory and attitude conditions during atmospheric entry. Strategies and current technological challenges for a human rated Entry, Descent, and Landing to the Martian surface are presented in this study.

Prabhakar Subrahmanyam↗

Back to Basics: First Principles of HRA

Human Reliability Analysis (HRA) continues to suffer from a myriad of technical gaps in its foundations. Core issues have been widely discussed but remain largely unaddressed, although the path taken to this point was paved with good intentions. Now, while this characterization may seem dramatic it is not intended to throw doubt onto HRA as a discipline or to diminish the value it has on increasing safety and resilience in nuclear power. Rather, it is intended to communicate that HRA has some serious challenges and is desperately in need of a critical evaluation. To quote Dougherty [1], “the misrepresentations that will undoubtedly arise may serve to provoke the necessary creativity.” What bears noting is that in the last thirty years since Alan Swain declared that “no adequate HRA had ever been done,” there has been little effort to correct the issues he highlighted. Human factors practice is still poorly implemented, many complex systems are fundamentally incompatible with reliable human performance, and there remains poor foundational support for many of the assumptions within HRA. We propose three categories of issues in HRA, philosophical, theoretical, and empirical and potential solutions to these issues.

22 GENERAL STUDIES OF NUCLEAR REACTORS↗

The Use 0f AVIRIS Imagery To Assess Clay Mineralogy And Debris-Flow Potential In Cataract Canyon, Utah: A Preliminary Report

Worldwide debris flows destroy property and take human lives every year (Costa, 1984). As a result of extensive property damage and loss of life there is a pressing need to go beyond just describing the nature and extent of debris flows as they occur. Most of the research into debris-flow initiation has centered on rainfall, slope angle, and existing debris-flow deposits (Costa and Wieczorek, 1987). The factor of source lithology has been recently addressed by studies in the sedimentary terranes of Grand Canyon (Webb et al., 1996; Griffiths et al., 1996) and on the Colorado Plateau as a whole.3 On the Colorado Plateau shales dominated by kaolinite and illite clays are significantly more likely to be recent producers of debris-flows than are shales in which smectite clays dominate.3 Establishing the location of shales and colluvial deposits containing kaolinite and illite clays in sedimentary terranes on the Colorado Plateau is essential to predicting where debris flows are likely to occur. AVIRIS imagery can be used to distinguish between types of clay minerals (Chabrillat et al., 2001), providing the basis for surface-materials maps. The ultimate product of this study will be a model that can be used to estimate the debris-flow hazard in Cataract Canyon, Utah. This model will be based on GIS overlay analysis of debris-flow initiation factor maps, including surface-materials maps derived from AVIRIS data.

Rudd, Lawrence↗

An approach to market analysis for lighter than air transportation of freight

An approach is presented to marketing analysis for lighter than air vehicles in a commercial freight market. After a discussion of key characteristics of supply and demand factors, a three-phase approach to marketing analysis is described. The existing transportation systems are quantitatively defined and possible roles for lighter than air vehicles within this framework are postulated. The marketing analysis views the situation from the perspective of both the shipper and the carrier. A demand for freight service is assumed and the resulting supply characteristics are determined. Then, these supply characteristics are used to establish the demand for competing modes. The process is then iterated to arrive at the market solution.

Roberts, P. O.↗

The Ultimate Factor of Safety for Aircraft and Spacecraft Its History, Applications and Misconceptions

The ultimate factor of safety (FOSULT) concept used in aircraft and spacecraft has evolved over many decades. Currently an FOSULT 1.5 is the FAR‐mandated value for aircraft while an FOSULT of 1.4 has been used in various spacecraft. This paper was motivated by the desire to concisely explain the origins, proper interpretation and application of the ultimate factor of safety concept, since the authors have seen throughout their careers many misconceptions and incorrect applications of this concept. The history of the ultimate factor of safety concept is briefly summarized, the proper application of the factor of safety in aircraft design, structural analysis and operations is covered in detail, examples of limit load exceedance in aircraft and spacecraft are discussed, the evolution of the 1.4 FOSULT for spacecraft is described and some misconceptions regarding the ultimate factor of safety concept are addressed. It is hoped that this paper can be a summary resource for engineers to understand the origin, purpose and proper application of the ultimate factor of safety.

Zipay, John J.↗

Near-Efficient and Non-Asymptotic Multiway Inference

We establish non-asymptotic efficiency guarantees for tensor decomposition–based inference in count data models. Under a Poisson framework, we consider two related goals: (i) parametric inference , the estimation of the full distributional parameter tensor, and (ii) multiway analysis , the recovery of its canonical polyadic (CP) decomposition factors. Our main result shows that in the rank-one setting, a rank-constrained maximum-likelihood estimator achieves multiway analysis with variance matching the Cramér–Rao Lower Bound (CRLB) up to absolute constants and logarithmic factors. This provides a general framework for studying “near-efficient” multiway estimators in finite-sample settings. For higher ranks, we illustrate that our multiway estimator may not attain the CRLB; nevertheless, CP-based parametric inference remains nearly minimax optimal, with error bounds that improve on prior work by offering more favorable dependence on the CP rank. Numerical experiments corroborate near-efficiency in the rank-one case and highlight the efficiency gap in higher-rank scenarios.

97 MATHEMATICS AND COMPUTING↗

A Study on Effect of Tuning Capacitor in Dynamic Radioisotope Power Systems

This study presents the effect of tuning capacitors on the power factor and power output of a gas spring-based free-piston Stirling convertor—Advanced Stirling convertor—when the convertor runs on a forced-oscillation type controller. More specifically, this paper studies how off-nominal tuning capacitance values affects the Stirling convertor in terms of the piston stability and electrical power output, when the convertor is operated by forced-oscillation type controller, such as an alternating current bus controller, and operating conditions of the convertor are unchanged. It has been widely known that tuning capacitors are important circuit components to stabilize the piston amplitude of the free-piston Stirling convertors and correct the power factor for high electrical power output. Recent modeling analysis conducted by NASA Glenn Research Center, however, suggests that while the stability of the convertor is affected by the tuning capacitor value, the power factor and the electrical power output are not affected by the tuning capacitor value, as long as the convertor is controlled by a forced-oscillation controller and the convertor operating conditions, such as the hot-end/cold-end temperatures, piston amplitude, and operating frequency, are unchanged. To verify the modeling analysis results, a test setup is devised where the convertor is controlled by a forced-oscillation controller and the piston amplitude, hot-end temperature, and charge pressure are controlled to 4mm, 760˚C, and 485psig, respectively, while the tuning capacitor value is changed from its nominal value, 840μF to 1040μF and 1240μF. Furthermore, the vibration test results of the same convertor with the three tuning capacitor values are also presented in this paper to show the effect of the tuning capacitor value on the convertor stability. The test results suggest that when the convertor is controlled by a forced-oscillation, the tuning capacitor value determines the convertor stability but not the power factor nor electrical power output. Therefore, the tuning capacitance value in forced-oscillation controllers should be determined based on the convertor stability, system mass, and volume.

Free-Piston Stirling Convertor Controller↗

Power Operations of the Mars Exploration Rovers

The rovers of the National Aeronautics and Space Administration’s (NASA) Mars Exploration Rovers (MER) project, Spirit (MER-A) and Opportunity (MER-B), safely landed on the surface of Mars three weeks apart during January 2004. Spirit and Opportunity were built and operated by the Jet Propulsion Laboratory (JPL), which is managed by the California Institute of Technology (Caltech) for NASA. Spirit landed at Gusev Crater, 14.8 degrees south of the equator, and operated continuously on the surface of Mars from January 4, 2004 until last contact from Spirit on March 22, 2010. Opportunity landed at Meridiani Planum, a location 2.5 degrees south of the Martian equator. Opportunity operated continuously on the Martian surface from January 25, 2004 until the last received transmission from Opportunity on June 10, 2018. The goal of the MER project was to determine if Mars ever had a habitable environment, in particular, if it ever had water. During their missions, both Spirit and Opportunity found evidence that liquid water once flowed on the surface of Mars. Both Spirit and Opportunity used a 1.33 m2 triple-junction solar array as their power sources. Based on observations of the original Mars rover, the Sojourner rover of the Mars Pathfinder mission that landed on Mars on July 4, 1997, the expectation was that the Martian dust would rapidly accumulate on the solar arrays of Spirit and Opportunity, and that the rovers would not have enough energy to continue operations after 90 Martian days (sols). Instead, due in part to lower dust accumulation rates than expected and numerous dust cleaning events, the Spirit and Opportunity rovers continued to operate on the Martian surface for over 2000 sols (MER-A) and 5000 sols (MER-B), respectively. During this time, the rovers experienced multiple Martian winters and several dust storms. Because the sources of solar array energy loss were known, the solar array energy output offered a method to scientifically estimate the loading and aeolian removal of dust from the solar arrays each sol. The MER Power operations team called this value that they calculated the solar array Dust Factor (DF). Dust Factor was defined as the fraction of sunlight that penetrates the accumulated dust on the surface of the solar array. A Dust Factor of 1.0 would indicate that the solar array was perfectly clean. A Dust Factor of 0.6 would indicate that only 60% of the available sunlight was able to penetrate the accumulated dust on the solar arrays. The MER Power subsystem operations team used the Multi-Mission Power Analysis Tool (MMPAT) to perform these Dust Factor calculations. The MMPAT software tool modeled the behavior of the solar arrays and the batteries as they interacted with the spacecraft power loads over the mission timeline. MMPAT also had knowledge (through telemetry and user inputs) of telemetered Power subsystem voltages and currents, atmospheric opacity (Tau), rover surface location, rover attitude, the planetary tilt and distance of Mars from the sun based on day of year, the instantaneous elevation of the sun based on time of day, temperatures (internal and external), terrain masking, and shadowing (due to the camera mast and antennas). Once all of the known sources of array energy loss are accounted, the remaining difference between the expected array energy and the actual array energy determines the solar array Dust Factor. The assumptions made while determining the Dust Factor are 1) that the single measured atmospheric opacity value (Tau) is constant over the course of the entire sol, 2) there is no measurable solar cell degradation, 3) there are no shorted strings and therefore, 4) all unexpected solar array energy losses are due to accumulated dust on the solar array. Although it cannot provide an absolute measure of dust loading, the determination of solar array Dust Factor provides a useful way of tracking dust accumulation and aeolian dust removal trends on Martian spacecraft. Any spacecraft on the Martian surface is vulnerable to dust, especially solar-powered spacecraft. The Spirit and Opportunity rovers were operational on the Martian surface for much longer than expected due in part to aeolian removal of dust from their solar arrays. The first few dust removal events were a pleasant surprise to the MER operations teams; however, over time a pattern began to arise. In over three Mars Years on the surface, the Power operations team tracked the solar array Dust Factor at Gusev Crater (location of MER-A) and observed that there were several significant dust removal and deposition events in Mars Year (MY) 27, even in the absence of a large dust storm. In MY 28 at Gusev Crater, the large atmospheric opacity (Tau) increase lagged significant dust removal events. Overall at Gusev Crater, there was a pattern of steady dust accumulation on the solar arrays, with a small number of significant dust cleaning events. At Meridiani Planum, where Opportunity rover operated for over seven Mars Years (late MY 26 to mid MY 34), a clear and consistent pattern of dust movement emerged. Meridiani Planum had a predictable, seasonally dependent pattern of gradual and continuous dust accumulation and removal. In summary, this paper explains the reasons for the development of the solar array Dust Factor and how it was used in mission operations. In particular, this paper describes the MMPAT software package, how it models array energy, including the important assumptions, model inputs and sources of error. And finally, this paper will show how the calculated solar array Dust Factor was used at Gusev Crater and Meridiani Planum to predict dust accumulation rates and weather patterns, and the importance of this generated data set for current and future solar- powered missions to Mars, such as the InSight lander and the planned Mars Sample Return rover.

Chin, Keith B.↗

Investigations on the Thermal Stability and Kinetics of Biolubricants Synthesized from Different Types of Vegetable Oils

Petroleum-based lubricants raise environmental concerns due to their non-biodegradability and toxicity, whereas biobased lubricants underperform owing to low thermal stability. This study examined and compared three vegetable oils, along with their chemically modified versions, to better understand their suitability as biolubricants. High oleic soybean oil (HOSOY), regular soybean oil (RSOY), and waste cooking oil (WCO) were subjected to chemical modification, where isopropyl groups were attached to the fatty acid chains of the oils to produce branched oils, i.e., b-HOSOY, b-RSOY, and b-WCO. The detailed kinetic study of each regular and modified sample was investigated using thermogravimetric analysis. The kinetic parameters, such as the activation energies, reaction rate, and pre-exponential factor, were generated via Friedman methods. The differential thermal gravimetric (DTG) analysis showed low volatilization at the onset temperature in each modified oil as compared with the unmodified samples under an oxidative environment. Furthermore, the comparative kinetic studies demonstrated the enhanced thermoxidative stability of the modified products relative to their unaltered counterparts. Among the tested oils, the b-RSOY showed an average activation energy of 325 kJ/mol, followed by the b-WCO: 300 kJ/mol and the b-HOSOY: 251 kJ/mol, indicating the most stable modified product under an oxidative environment. For all the samples, the pre-exponential factors were in good agreement with the activation energies, which validates that finding the pre-exponential components is crucial to the kinetic analysis.

Sarker, Majher I. (ORCID:0000000299509274)↗

Stress analysis of cracks

Elastic stress analyses of cracked bodies represented by stress intensity factor method - fracture mechanics

STRESS ANALYSIS↗

LAI, EVI, NDVI, and kNDVI in 23 pantropical forests affected by 21 cyclones

Statement of purpose: Cyclones alter the function and composition of tropical forests, making effects of intensifying cyclones on carbon-rich forests a critical topic of study. Here, we quantified cyclone-induced damage and recovery of 21 cyclone disturbances affecting 23 pantropical forest sites between 1988-2017 utilizing leaf area index (LAI), enhanced vegetation index (EVI), normalized difference vegetation index (NDVI), and transformed NDVI (kNDVI) values from Google Earth Engine. Field observations collected in a meta-analysis (Bomfim et al., 2022, in review) were used to ground-truth and test effects of soil resource availability and disturbance factors on damage and recovery. This meta-analysis also served as the basis to begin vegetation index extraction, utilizing unique site and date combinations, from tropical forests effect by cyclone disturbances. We began collecting NDVI (5km resolution) from the NOAA Climate Data Record (CDR) of AVHRR Normalized Difference Vegetation Index (NDVI), Version 5 data product (Vermote, 2019) for all case studies included, 42. Next, we began extracting Landsat data from Landsat 4, 5, and 8, courtesy of the U.S. Geological Survey, in search of higher resolution data. We selected a 3 by 3 Landsat pixel area, leading to a 90m resolution data extraction. The specific imagery used includes Landsat 4 USGS Landsat 4 TM Collection 1 Tier 1 TOA (top of atmosphere) Reflectance, Landsat 5 USGS Landsat 5 TM (thematic mapper) Collection 1 Tier 1 TOA Reflectance, and Landsat 8 USGS Landsat 8 Collection 1 Tier 1 TOA Reflectance. Within Google Earth Engine, we selected the date and location (latitude and longitude), calculated NDVI, kNDVI, and EVI utilizing Landsat bands (see metadata_NGEE-tropics_cyclones), and extracted post- and pre-cyclone values for each case study to calculate cyclone-induced change in the vegetative index. Due to limited spatial resolution of Landsat remote sensing data, MODIS products were investigated next. First, the MOD13Q1.006 Terra Vegetation Indices 16-Day Global 250m product was used to extract 250m EVI and NDVI (Didan, 2015) and then the MCD15A3H.006 MODIS Leaf Area Index/FPAR 4-Day Global 500m product product was used to extract LAI 500m (Myneni et al., 2015). Pre- and post-cyclone values, change in the vegetative index, and standard deviation for all values are included in the main csv (see case_study_data.csv) for all vegetative indices collected, including LAI 500m, EVI 250m, NDVI 250m, NDVI 90m, kNDVI 90m, EVI 90m, and NDVI 5km. Lastly, recovery values were calculated utilizing a standardization method (see metadata_NGEE-tropics_cyclones) and recovery values for MODIS (see MODIS_recovery.csv) and Landsat (Landsat_recovery.csv) data are included.

54 ENVIRONMENTAL SCIENCES↗

Telemetry Monitoring and Display Using LabVIEW

The Measurement Technology Center of the Instrumentation Section configures automated data acquisition systems to meet the diverse needs of JPL's experimental research community. These systems are based on personal computers or workstations (Apple, IBM/Compatible, Hewlett-Packard, and Sun Microsystems) and often include integrated data analysis, visualization and experiment control functions in addition to data acquisition capabilities. These integrated systems may include sensors, signal conditioning, data acquisition interface cards, software, and a user interface. Graphical programming is used to simplify configuration of such systems. Employment of a graphical programming language is the most important factor in enabling the implementation of data acquisition, analysis, display and visualization systems at low cost. Other important factors are the use of commercial software packages and off-the-shelf data acquisition hardware where possible. Understanding the experimenter's needs is also critical. An interactive approach to user interface construction and training of operators is also important. One application was created as a result of a competative effort between a graphical programming language team and a text-based C language programming team to verify the advantages of using a graphical programming language approach. With approximately eight weeks of funding over a period of three months, the text-based programming team accomplished about 10% of the basic requirements, while the Macintosh/LabVIEW team accomplished about 150%, having gone beyond the original requirements to simulate a telemetry stream and provide utility programs. This application verified that using graphical programming can significantly reduce software development time. As a result of this initial effort, additional follow-on work was awarded to the graphical programming team.

graphical user interface LabVIEW graphical program↗

Diffuse continuum gamma rays from the Galaxy observed by COMPTEL

The diffuse Galactic continuum gamma-ray emission has been studied using the full Sky Survey from COMPTEL on the Compton Observatory CGRO. The diffuse emission appears to be visible in the whole 0.75-30 MeV range covered by the instrument, although a considerable contribution from unresolved point sources cannot be excluded. A correlation analysis using HI and CO surveys of the Galaxy is used to derive the Galactic emissivity spectrum, and this is consistent with a smooth continuation to the spectrum at higher energies derived by a similar analysis of COS-B data. The apparent conversion factor from integrated CO temperature to molecular hydrogen column density can also be determined from the correlation analysis. The value obtained is consistent with results from COS-B and other non-gamma-ray methods. Calculations of the emissivity spectrum from bremsstrahlung from a cosmic-ray electron spectrum based on propagation models are compared with the observations.

Strong, A. W.↗

Real-Time, Adaptive Radiological Anomaly Detection and Isotope Identification Using Non-Negative Matrix Factorization

Spectroscopic anomaly detection and isotope identification algorithms are integral components in nuclear nonproliferation applications such as search operations. The task is especially challenging in the case of mobile detector systems because the observed gamma-ray background changes more than for a static detector system, and a pretrained background model can easily find itself out of domain. The result is that algorithms may exceed their intended false alarm rate or sacrifice detection sensitivity to maintain the desired false alarm rate. Non-negative matrix factorization (NMF) is a powerful tool for spectral anomaly detection and identification, but, like many similar algorithms that rely on data-driven background models, in its conventional implementation, it is unable to update in real time to account for environmental changes that affect the background spectroscopic signature. Here, we have developed a novel NMF-based algorithm that periodically updates its background model to accommodate changing environmental conditions. The adaptive NMF algorithm involves fewer assumptions about its environment, making it more generalizable than existing NMF-based methods while maintaining or exceeding detection performance on simulated and real-world datasets.

Anomaly detection↗

Assessment of a remote sensing-based model for predicting malaria transmission risk in villages of Chiapas, Mexico

A blind test of two remote sensing-based models for predicting adult populations of Anopheles albimanus in villages, an indicator of malaria transmission risk, was conducted in southern Chiapas, Mexico. One model was developed using a discriminant analysis approach, while the other was based on regression analysis. The models were developed in 1992 for an area around Tapachula, Chiapas, using Landsat Thematic Mapper (TM) satellite data and geographic information system functions. Using two remotely sensed landscape elements, the discriminant model was able to successfully distinguish between villages with high and low An. albimanus abundance with an overall accuracy of 90%. To test the predictive capability of the models, multitemporal TM data were used to generate a landscape map of the Huixtla area, northwest of Tapachula, where the models were used to predict risk for 40 villages. The resulting predictions were not disclosed until the end of the test. Independently, An. albimanus abundance data were collected in the 40 randomly selected villages for which the predictions had been made. These data were subsequently used to assess the models' accuracies. The discriminant model accurately predicted 79% of the high-abundance villages and 50% of the low-abundance villages, for an overall accuracy of 70%. The regression model correctly identified seven of the 10 villages with the highest mosquito abundance. This test demonstrated that remote sensing-based models generated for one area can be used successfully in another, comparable area.

Insect Vectors/growth & development↗

Improved Upper Limit on Degree-scale CMB B -mode Polarization Power from the 670 Square-degree POLARBEAR Survey

We report an improved measurement of the degree-scale cosmic microwave background B-mode angular-power spectrum over 670 deg 2 sky area at 150 GHz with Polarbear. In the original analysis of the data, errors in the angle measurement of the continuously rotating half-wave plate, a polarization modulator, caused significant data loss. By introducing an angle-correction algorithm, the data volume is increased by a factor of 1.8. We report a new analysis using the larger data set. We find the measured B-mode spectrum is consistent with the ΛCDM model with Galactic dust foregrounds. We estimate the contamination of the foreground by cross-correlating our data and Planck 143, 217, and 353 GHz measurements, where its spectrum is modeled as a power law in angular scale and a modified blackbody in frequency. We place an upper limit on the tensor-to-scalar ratio r < 0.33 at 95% confidence level after marginalizing over the foreground parameters.

79 ASTRONOMY AND ASTROPHYSICS↗

Large Contribution of Meteorological Factors to Inter-Decadal Changes in Regional Aerosol Optical Depth

Aerosol optical depth (AOD) has become a crucial metric for assessing global climate change. Although global and regional AOD trends have been studied extensively, it remains unclear what factors are driving the inter-decadal variations in regional AOD and how to quantify the relative contribution of each dominant factor. This study used a long-term (1980–2016) aerosol dataset from the Modern-Era Retrospective Analysis for Research and Applications, version 2 (MERRA-2) reanalysis, along with two satellite-based AOD datasets (MODIS/Terra and MISR) from 2001 to 2016, to investigate the long-term trends in global and regional aerosol loading. Statistical models based on emission factors and meteorological parameters were developed to identify the main factors driving the inter-decadal changes of regional AOD and to quantify their contribution. Evaluation of the MERRA-2 AOD with the ground-based measurements of AERONET indicated significant spatial agreement on the global scale (r= 0.85, root-mean-square error = 0.12, mean fractional error = 38.7 %, fractional gross error = 9.86 % and index of agreement = 0.94). However, when AOD observations from the China Aerosol Remote Sensing Network (CARSNET) were employed for independent verification, the results showed that MERRA-2 AODs generally underestimated CARSNET AODs in China (relative mean bias = 0.72 and fractional gross error =−34.3 %). In general, MERRA-2 was able to quantitatively reproduce the annual and seasonal AOD trends on both regional and global scales, as observed by MODIS/Terra, although some differences were found when compared to MISR. Over the 37-year period in this study, significant decreasing trends were observed over Europe and the eastern United States. In contrast, eastern China and southern Asia showed AOD increases, but the increasing trend of the former reversed sharply in the most recent decade. The statistical analyses suggested that the meteorological parameters explained a larger proportion of the AOD variability (20.4 %–72.8 %) over almost all regions of interest (ROIs) during 1980–2014 when compared with emission factors (0 %–56 %). Further analysis also showed that SO2 was the dominant emission factor, explaining 12.7 %–32.6 % of the variation in AOD over anthropogenic-aerosol-dominant regions, while black carbon or organic carbon was the leading factor over the biomass-burning-dominant (BBD) regions, contributing 24.0 %–27.7 % of the variation. Additionally, wind speed was found to be the leading meteorological parameter, explaining 11.8 %–30.3 % of the variance over the mineral-dust-dominant regions, while ambient humidity (including soil moisture and relative humidity) was the top meteorological parameter over the BBD regions, accounting for 11.7 %–35.5 % of the variation. The results of this study indicate that the variation in meteorological parameters is a key factor in determining the inter-decadal change in regional AOD.

Huizheng Che↗

Genome-wide profiling of histone (H3) lysine 4 (K4) tri-methylation (me3) under drought, heat, and combined stresses in switchgrass

Background: Switchgrass (Panicum virgatum L.) is a warm-season perennial (C4) grass identified as an important biofuel crop in the United States. It is well adapted to the marginal environment where heat and moisture stresses predominantly affect crop growth. However, the underlying molecular mechanisms associated with heat and drought stress tolerance still need to be fully understood in switchgrass. The methylation of H3K4 is often associated with transcriptional activation of genes, including stress-responsive. Therefore, this study aimed to analyze genome-wide histone H3K4-tri-methylation in switchgrass under heat, drought, and combined stress. Results: In total, ~ 1.3 million H3K4me3 peaks were identified in this study using SICER. Among them, 7,342; 6,510; and 8,536 peaks responded under drought (DT), drought and heat (DTHT), and heat (HT) stresses, respectively. Most DT and DTHT peaks spanned 0 to + 2000 bases from the transcription start site [TSS]. By comparing differentially marked peaks with RNA-Seq data, we identified peaks associated with genes: 155 DT-responsive peaks with 118 DT-responsive genes, 121 DTHT-responsive peaks with 110 DTHT-responsive genes, and 175 HT-responsive peaks with 136 HT-responsive genes. We have identified various transcription factors involved in DT, DTHT, and HT stresses. Gene Ontology analysis using the AgriGO revealed that most genes belonged to biological processes. Most annotated peaks belonged to metabolite interconversion, RNA metabolism, transporter, protein modifying, defense/immunity, membrane traffic protein, transmembrane signal receptor, and transcriptional regulator protein families. Further, we identified significant peaks associated with TFs, hormones, signaling, fatty acid and carbohydrate metabolism, and secondary metabolites. qRT-PCR analysis revealed the relative expressions of six abiotic stress-responsive genes (transketolase, chromatin remodeling factor-CDH3, fatty-acid desaturase A, transmembrane protein 14C, beta-amylase 1, and integrase-type DNA binding protein genes) that were significantly (P < 0.05) marked during drought, heat, and combined stresses by comparing stress-induced against un-stressed and input controls. Conclusion: Our study provides a comprehensive and reproducible epigenomic analysis of drought, heat, and combined stress responses in switchgrass. Significant enrichment of H3K4me3 peaks downstream of the TSS of protein-coding genes was observed. In addition, the cost-effective experimental design, modified ChIP-Seq approach, and analyses presented here can serve as a prototype for other non-model plant species for conducting stress studies.

59 BASIC BIOLOGICAL SCIENCES↗