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At least 1,027 records · Page 57

Comparative proteomic analysis of Desulfotomaculum reducens MI-1: Insights into the metabolic versatility of a gram-positive sulfate- and metal-reducing bacterium

In this study, the proteomes of the metabolically versatile and poorly characterized Gram-positive bacterium Desulfotomaculum reducens MI-1 were compared across four cultivation conditions including sulfate reduction, soluble Fe(III) reduction, insoluble Fe(III) reduction, and pyruvate fermentation. Collectively across conditions, we observed at high confidence ~38% of genome-encoded proteins. Here, we focus on proteins that display significant differential abundance on conditions tested. To the best of our knowledge, this is the first full-proteome study focused on a Gram-positive organism cultivated either on sulfate or metal-reducing conditions. Several proteins with uncharacterized function encoded within heterodisulfide reductase ( hdr )-containing loci were upregulated on either sulfate (Dred_0633-4, Dred_0689-90, and Dred_1325-30) or Fe(III)-citrate-reducing conditions (Dred_0432-3 and Dred_1778-84). Two of these hdr -containing loci display homology to recently described flavin-based electron bifurcation (FBEB) pathways (Dred_1325-30 and Dred_1778-84). Additionally, we propose that a cluster of proteins, which is homologous to a described FBEB lactate dehydrogenase (LDH) complex, is performing lactate oxidation in D. reducens (Dred_0367-9). Analysis of the putative sulfate reduction machinery in D. reducens revealed that most of these proteins are constitutively expressed across cultivation conditions tested. In addition, peptides from the single multiheme c-type cytochrome (MHC) in the genome were exclusively observed on the insoluble Fe(III) condition, suggesting that this MHC may play a role in reduction of insoluble metals.

59 BASIC BIOLOGICAL SCIENCES↗

Comparative Virulence and Genomic Analysis of Streptococcus suis Isolates

Streptococcus suis is a zoonotic bacterial swine pathogen causing substantial economic and health burdens to the pork industry. Mechanisms used by S. suis to colonize and cause disease remain unknown and vaccines and/or intervention strategies currently do not exist. Studies addressing virulence mechanisms used by S. suis have been complicated because different isolates can cause a spectrum of disease outcomes ranging from lethal systemic disease to asymptomatic carriage. The objectives of this study were to evaluate the virulence capacity of nine United States S. suis isolates following intranasal challenge in swine and then perform comparative genomic analyses to identify genomic attributes associated with swine-virulent phenotypes. No correlation was found between the capacity to cause disease in swine and the functional characteristics of genome size, serotype, sequence type (ST), or in vitro virulence-associated phenotypes. A search for orthologs found in highly virulent isolates and not found in non-virulent isolates revealed numerous predicted protein coding sequences specific to each category. While none of these predicted protein coding sequences have been previously characterized as potential virulence factors, this analysis does provide a reliable one-to-one assignment of specific genes of interest that could prove useful in future allelic replacement and/or functional genomic studies. Collectively, this report provides a framework for future allelic replacement and/or functional genomic studies investigating genetic characteristics underlying the spectrum of disease outcomes caused by S. suis isolates.

59 BASIC BIOLOGICAL SCIENCES↗

Assessing the Level of Inter-Sectoral Policy Integration for Governance in the Water–Energy Nexus: A Comparative Study of Los Angeles and Beijing

As concerns around water scarcity and energy security increase, so too has interest in the connections between these resources, through a concept called the water–energy nexus. Efforts to improve the integration of water and energy management and to understand their cross-sector relevance are growing. In particular, this paper develops a better empirical understanding on the extent to which governance settings hinder and/or enable policy coherence between the water and energy sectors through a comparative analysis of two case studies, namely, Los Angeles County, California, the United States, and the city of Beijing, China. This paper examines the extent to which the institutional context enables policy coordination within (vertically) and between (horizontally) the water and energy sectors in Beijing and Los Angeles. To do so, we propose a framework for analyzing policy integration for the water energy nexus based on environmental policy integration (EPI). The results highlight the multiple and flexible approaches of EPI in nexus governance, not least with regards to horizontal and vertical policy integration, but also in terms of explicit (i.e., intended) and implicit (i.e., unintended) coordination. The level of nexus-focused policy integration is highly dependent on the motivation at the local context and the criteria to evaluate policy success in each sector.

29 ENERGY PLANNING, POLICY, AND ECONOMY↗

A COMPARATIVE STUDY OF KNOTS OF STAR FORMATION IN INTERACTING VERSUS SPIRAL GALAXIES

Interacting galaxies are known to have higher global rates of star formation on average than normal galaxies, relative to their stellar masses. Using UV and IR photometry combined with new and published Hα images, we have compared the star formation rates (SFRs) of ∼700 star forming complexes in 46 nearby interacting galaxy pairs with those of regions in 39 normal spiral galaxies. The interacting galaxies have proportionally more regions with high SFRs than the spirals. The most extreme regions in the interacting systems lie at the intersections of spiral/tidal structures, where gas is expected to pile up and trigger star formation. Published Hubble Space Telescope images show unusually large and luminous star clusters in the highest luminosity regions. The SFRs of the clumps correlate with measures of the dust attenuation, consistent with the idea that regions with more interstellar gas have more star formation. For the clumps with the highest SFRs, the apparent dust attenuation is consistent with the Calzetti starburst dust attenuation law. This suggests that the high luminosity regions are dominated by a central group of young stars surrounded by a shell of clumpy interstellar gas. In contrast, the lower luminosity clumps are bright in the UV relative to Hα, suggesting either a high differential attenuation between the ionized gas and the stars, or a post-starburst population bright in the UV but faded in Hα. The fraction of the global light of the galaxies in the clumps is higher on average for the interacting galaxies than for the spirals. Thus either star formation in interacting galaxies is “clumpier” on average, or the star forming regions in interacting galaxies are more luminous, dustier, or younger on average.

79 ASTRONOMY AND ASTROPHYSICS↗

COMPARING [C ii], H i, AND CO DYNAMICS OF NEARBY GALAXIES

The H i and CO components of the interstellar medium (ISM) are usually used to derive the dynamical mass M{sub dyn} of nearby galaxies. Both components become too faint to be used as a tracer in observations of high-redshift galaxies. In those cases, the 158 μm line of atomic carbon ([C ii]) may be the only way to derive M{sub dyn}. As the distribution and kinematics of the ISM tracer affects the determination of M{sub dyn}, it is important to quantify the relative distributions of H i, CO, and [C ii]. H i and CO are well-characterized observationally, however, for [C ii] only very few measurements exist. Here we compare observations of CO, H i, and [C ii] emission of a sample of nearby galaxies, drawn from the HERACLES, THINGS, and KINGFISH surveys. We find that within R {sub 25}, the average [C ii] exponential radial profile is slightly shallower than that of the CO, but much steeper than the H i distribution. This is also reflected in the integrated spectrum (“global profile”), where the [C ii] spectrum looks more like that of the CO than that of the H i. For one galaxy, a spectrally resolved comparison of integrated spectra was possible; other comparisons were limited by the intrinsic line-widths of the galaxies and the coarse velocity resolution of the [C ii] data. Using high-spectral-resolution SOFIA [C ii] data of a number of star forming regions in two nearby galaxies, we find that their [C ii] linewidths agree better with those of the CO than the H i. As the radial extent of a given ISM tracer is a key input in deriving M{sub dyn} from spatially unresolved data, we conclude that the relevant length-scale to use in determining M{sub dyn} based on [C ii] data, is that of the well-characterized CO distribution. This length scale is similar to that of the optical disk.

79 ASTRONOMY AND ASTROPHYSICS↗

HST emission line galaxies at z ∼ 2: Comparing physical properties of Lyman alpha and optical emission line selected galaxies

We compare the physical and morphological properties of z ∼ 2 Lyα emitting galaxies (LAEs) identified in the HETDEX Pilot Survey and narrow band studies with those of z ∼ 2 optical emission line selected galaxies (oELGs) identified via HST WFC3 infrared grism spectroscopy. Both sets of galaxies extend over the same range in stellar mass (7.5<logM/M{sub ⊙}<10.5), size (0.5 < R < 3.0 kpc), and star formation rate (∼1<SFR<100 M{sub ⊙} yr{sup −1}). Remarkably, a comparison of the most commonly used physical and morphological parameters—stellar mass, half-light radius, UV slope, SFR, ellipticity, nearest neighbor distance, star formation surface density, specific SFR, [O iii] luminosity, and [O iii] equivalent width—reveals no statistically significant differences between the populations. This suggests that the processes and conditions which regulate the escape of Lyα from a z ∼ 2 star-forming galaxy do not depend on these quantities. In particular, the lack of dependence on the UV slope suggests that Lyα emission is not being significantly modulated by diffuse dust in the interstellar medium. We develop a simple model of Lyα emission that connects LAEs to all high-redshift star-forming galaxies where the escape of Lyα depends on the sightline through the galaxy. Using this model, we find that mean solid angle for Lyα escape is Ω{sub Lyα}=2.4±0.8 steradians; this value is consistent with those calculated from other studies.

79 ASTRONOMY AND ASTROPHYSICS↗

CHROMOSPHERIC HEATING BY ACOUSTIC WAVES COMPARED TO RADIATIVE COOLING

Acoustic and magnetoacoustic waves are among the possible candidate mechanisms that heat the upper layers of the solar atmosphere. A weak chromospheric plage near the large solar pore NOAA 11005 was observed on 2008 October 15, in the Fe i 617.3 nm and Ca ii 853.2 nm lines of the Interferometric Bidimemsional Spectrometer attached to the Dunn Solar Telescope. In analyzing the Ca ii observations (with spatial and temporal resolutions of 0.″4 and 52 s) the energy deposited by acoustic waves is compared to that released by radiative losses. The deposited acoustic flux is estimated from the power spectra of Doppler oscillations measured in the Ca ii line core. The radiative losses are calculated using a grid of seven one-dimensional hydrostatic semi-empirical model atmospheres. The comparison shows that the spatial correlation of the maps of radiative losses and acoustic flux is 72%. In a quiet chromosphere, the contribution of acoustic energy flux to radiative losses is small, only about 15%. In active areas with a photospheric magnetic-field strength between 300 and 1300 G and an inclination of 20°–60°, the contribution increases from 23% (chromospheric network) to 54% (a plage). However, these values have to be considered as lower limits and it might be possible that the acoustic energy flux is the main contributor to the heating of bright chromospheric network and plages.

79 ASTRONOMY AND ASTROPHYSICS↗

Young stars and ionized nebulae in M83: comparing chemical abundances at high metallicity

We present spectra of 14 A-type supergiants in the metal-rich spiral galaxy M83. We derive stellar parameters and metallicities and measure a spectroscopic distance modulus μ=28.47±0.10 (4.9 ± 0.2 Mpc), in agreement with other methods. We use the stellar characteristic metallicity of M83 and other systems to discuss a version of the galaxy mass–metallicity relation that is independent of the analysis of nebular emission lines and the associated systematic uncertainties. We reproduce the radial metallicity gradient of M83, which flattens at large radii, with a chemical evolution model, constraining gas inflow and outflow processes. We carry out a comparative analysis of the metallicities we derive from the stellar spectra and published H ii region line fluxes, utilizing both the direct, T{sub e}-based method and different strong-line abundance diagnostics. The direct abundances are in relatively good agreement with the stellar metallicities, once we apply a modest correction to the nebular oxygen abundance due to depletion onto dust. Popular empirically calibrated strong-line diagnostics tend to provide nebular abundances that underestimate the stellar metallicities above the solar value by ∼0.2 dex. This result could be related to difficulties in selecting calibration samples at high metallicity. The O3N2 method calibrated by Pettini and Pagel gives the best agreement with our stellar metallicities. We confirm that metal recombination lines yield nebular abundances that agree with the stellar abundances for high-metallicity systems, but find evidence that in more metal-poor environments they tend to underestimate the stellar metallicities by a significant amount, opposite to the behavior of the direct method.

79 ASTRONOMY AND ASTROPHYSICS↗

The grism lens-amplified survey from space (glass). VI. Comparing the mass and light in MACS J0416.1-2403 using frontier field imaging and glass spectroscopy

We present a model using both strong and weak gravitational lensing of the galaxy cluster MACS J0416.1-2403, constrained using spectroscopy from the Grism Lens-Amplified Survey from Space (GLASS) and Hubble Frontier Fields (HFF) imaging data. We search for emission lines in known multiply imaged sources in the GLASS spectra, obtaining secure spectroscopic redshifts of 30 multiple images belonging to 15 distinct source galaxies. The GLASS spectra provide the first spectroscopic measurements for five of the source galaxies. The weak lensing signal is acquired from 884 galaxies in the F606W HFF image. By combining the weak lensing constraints with 15 multiple image systems with spectroscopic redshifts and nine multiple image systems with photometric redshifts, we reconstruct the gravitational potential of the cluster on an adaptive grid. The resulting map of total mass density is compared with a map of stellar mass density obtained from the deep Spitzer Frontier Fields imaging data to study the relative distribution of stellar and total mass in the cluster. We find that the projected stellar mass to total mass ratio, f {sub ⋆}, varies considerably with the stellar surface mass density. The mean projected stellar mass to total mass ratio is 〈f{sub ⋆}〉=0.009±0.003 (stat.), but with a systematic error as large as 0.004–0.005, dominated by the choice of the initial mass function. We find agreement with several recent measurements of f {sub ⋆} in massive cluster environments. The lensing maps of convergence, shear, and magnification are made available to the broader community in the standard HFF format.

79 ASTRONOMY AND ASTROPHYSICS↗

Shield: comparing gas and star formation in low-mass galaxies

We analyze the relationships between atomic, neutral hydrogen (H i) and star formation (SF) in the 12 low-mass SHIELD galaxies. We compare high spectral (∼0.82 km s{sup −1} ch{sup −1}) and spatial resolution (physical resolutions of 160–640 pc) H i imaging from the VLA with Hα and far-ultraviolet imaging. We quantify the degree of co-spatiality between star-forming regions and regions of high H i column densities. We calculate the global star formation efficiencies (SFE; Σ{sub SFR} / Σ{sub HI}) and examine the relationships among the SFE and H i mass, H i column density, and star formation rate (SFR). The systems are consuming their cold neutral gas on timescales of order a few gigayears. While we derive an index for the Kennicutt–Schmidt relation of N ≈ 0.68 ± 0.04 for the SHIELD sample as a whole, the values of N vary considerably from system to system. By supplementing SHIELD results with those from other surveys, we find that H i mass and UV-based SFR are strongly correlated over five orders of magnitude. Identification of patterns within the SHIELD sample allows us to bin the galaxies into three general categories: (1) mainly co-spatial H i and SF regions, found in systems with the highest peak H i column densities and highest total H i masses; (2) moderately correlated H i and SF regions, found in systems with moderate H i column densities; and (3) obvious offsets between H i and SF peaks, found in systems with the lowest total H i masses. SF in these galaxies is dominated by stochasticity and random fluctuations in their ISM.

79 ASTRONOMY AND ASTROPHYSICS↗

COMPARING CORONAL AND HELIOSPHERIC MAGNETIC FIELDS OVER SEVERAL SOLAR CYCLES

Here we use the PFSS model and photospheric data from Wilcox Solar Observatory, SOHO /MDI, SDO/HMI, and SOLIS to compare the coronal field with heliospheric magnetic field measured at 1 au, compiled in the NASA/NSSDC OMNI 2 data set. We calculate their mutual polarity match and the power of the radial decay, p , of the radial field using different source surface distances and different number of harmonic multipoles. We find the average polarity match of 82% for the declining phase, 78%–79% for maxima, 76%–78% for the ascending phase, and 74%–76% for minima. On an average, the source surface of 3.25 R{sub S} gives the best polarity match. We also find strong evidence for solar cycle variation of the optimal source surface distance, with highest values (3.3 R{sub S}) during solar minima and lowest values (2.6 R{sub S}–2.7 R{sub S}) during the other three solar cycle phases. Raising the number of harmonic terms beyond 2 rarely improves the polarity match, showing that the structure of the HMF at 1 au is most of the time rather simple. All four data sets yield fairly similar polarity matches. Thus, polarity comparison is not affected by photospheric field scaling, unlike comparisons of the field intensity.

79 ASTRONOMY AND ASTROPHYSICS↗

Indication of the Hanle Effect by Comparing the Scattering Polarization Observed by CLASP in the Ly α and Si iii 120.65 nm Lines

The Chromospheric Lyman-Alpha Spectro-Polarimeter is a sounding rocket experiment that has provided the first successful measurement of the linear polarization produced by scattering processes in the hydrogen Ly α line (121.57 nm) radiation of the solar disk. In this paper, we report that the Si iii line at 120.65 nm also shows scattering polarization and we compare the scattering polarization signals observed in the Ly α and Si iii lines in order to search for observational signatures of the Hanle effect. We focus on four selected bright structures and investigate how the U / I spatial variations vary between the Ly α wing, the Ly α core, and the Si iii line as a function of the total unsigned photospheric magnetic flux estimated from Solar Dynamics Observatory /Helioseismic and Magnetic Imager observations. In an internetwork region, the Ly α core shows an antisymmetric spatial variation across the selected bright structure, but it does not show it in other more magnetized regions. In the Si iii line, the spatial variation of U / I deviates from the above-mentioned antisymmetric shape as the total unsigned photospheric magnetic flux increases. A plausible explanation of this difference is the operation of the Hanle effect. We argue that diagnostic techniques based on the scattering polarization observed simultaneously in two spectral lines with very different sensitivities to the Hanle effect, like Ly α and Si iii, are of great potential interest for exploring the magnetism of the upper solar chromosphere and transition region.

79 ASTRONOMY AND ASTROPHYSICS↗

A Comparative Observational Study of YSO Classification in Four Small Star-forming H ii Regions

We have developed a new young stellar object (YSO) identification and classification technique using mid-infrared Wide-field Infrared Survey Explorer (WISE) data. We compare this new technique with previous WISE YSO detection and classification methods that used either infrared colors or spectral energy distribution slopes. In this study, we also use the new technique to detect and examine the YSO population associated with four small H ii regions: KR 7, KR 81, KR 120, and KR 140. The relatively simple structure of these regions allows us to effectively use both spatial and temporal constraints to identify YSOs that are potential products of triggered star formation. We are also able to identify regions of active star formation around these H ii regions that are clearly not influenced by the H ii region expansion, and thus demonstrate that star formation is on-going on megayear timescales in some of these molecular clouds.

79 ASTRONOMY AND ASTROPHYSICS↗

The FluxCompensator: Making Radiative Transfer Models of Hydrodynamical Simulations Directly Comparable to Real Observations

When modeling astronomical objects throughout the universe, it is important to correctly treat the limitations of the data, for instance finite resolution and sensitivity. In order to simulate these effects, and to make radiative transfer models directly comparable to real observations, we have developed an open-source Python package called the FluxCompensator that enables the post-processing of the output of 3D Monte Carlo radiative transfer codes, such as Hyperion. With the FluxCompensator, realistic synthetic observations can be generated by modeling the effects of convolution with arbitrary point-spread functions, transmission curves, finite pixel resolution, noise, and reddening. Pipelines can be applied to compute synthetic observations that simulate observatories, such as the Spitzer Space Telescope or the Herschel Space Observatory . Additionally, this tool can read in existing observations (e.g., FITS format) and use the same settings for the synthetic observations. In this paper, we describe the package as well as present examples of such synthetic observations.

79 ASTRONOMY AND ASTROPHYSICS↗

Near-infrared diode laser based spectroscopic detection of ammonia: a comparative study of photoacoustic and direct optical absorption methods

A photoacoustic spectroscopic (PAS) and a direct optical absorption spectroscopic (OAS) gas sensor, both using continuous-wave room-temperature diode lasers operating at 1531.8 nm, were compared on the basis of ammonia detection. Excellent linear correlation between the detector signals of the two systems was found. Although the physical properties and the mode of operation of both sensors were significantly different, their performances were found to be remarkably similar, with a sub-ppm level minimum detectable concentration of ammonia and a fast response time in the range of a few minutes.

NASA Discipline Life Sciences Technologies↗