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All sourcesNASA NTRSDOE OSTI
NASA NTRS1996

Use of bed rest and head-down tilt to simulate spaceflight-induce immune system changes

Bed rest, both with and without head-down tilt, has been extensively used as an earth-bound analog to study physiologic effects mimicking those occurring in weightlessness during spaceflight. We have been able to show in six subjects that 4 weeks of head-down tilt bed rest induces a significant decrease in interleukin-2 secretion by PHA-stimulated T lymphocytes. Another study, lasting 113 days, with two subjects showed a decreased interleukin-2 receptor expression in PHA-stimulated peripheral blood mononuclear cells but a decreased interleukin-2 production in one subject only. Under the same conditions, interleukin-1 production was largely increased in both subjects. Several other immune parameters were also analyzed. Increased interleukin-1 production could contribute to bone mineral loss encountered during bed rest and decreased interleukin-2 secretion could play a role in the appearance of infectious diseases often observed during bed red.

NASA Discipline Neuroscience↗
DOE OSTI2025

Author Correction: Genome-guided isolation of the hyperthermophilic aerobe Fervidibacter sacchari reveals conserved polysaccharide metabolism in the Armatimonadota

Correction to: Nature Communicationshttps://doi.org/10.1038/s41467-024-53784-3, published online 4 November 2024 In the version of this article initially published, Table 1 did not include the properties of the taxa being proposed or refer directly to another location in the main manuscript describing the properties. As such, the original manuscript did not comply with Rule 27 (2)(c) of the ICNP. Also, Table 1 listed the order Fervidibacterales as the nomenclatural type for the class Fervidibacteria, which violates latest emended version of Rule 15 stating that the nomenclatural type for a class must be a genus. Below we provide a modification of Table 1 containing protologues with these errors corrected. We have also changed the order of the taxa in the table to meet the most common ordering. (Table presented.) Taxon names proposed under the ICNP Proposed taxon Etymology Description Genus Fervidibacter Fer.vi.di.bac’ter. L. masc. adj. fervidus, hot, steaming; N.L. masc. n. bacter, a rod; N.L. masc. n. Fervidibacter, a hot rod Thermophilic or hyperthermophilic inhabitants of freshwater thermal environments. All members are likely polysaccharide-degrading chemoheterotrophs with numerous carbohydrate-active enzymes encoded in their genomes. Aerobic, with high-affinity and/or low-affinity terminal oxidases present in the genomes. The oxidative pentose phosphate pathway and the tricarboxylic acid cycle are complete in genomes belonging to the genus. Gram-stain-negative and diderm cell envelope structure. Ovoid- to rod-shaped morphology. Spores are not formed. The genus is a distinct phylogenetic lineage in the family Fervidibacteraceae, the order Fervidibacterales, and the class Fervidibacteria in the phylum Armatimonadota. The type species is Fervidibacter sacchariT. Species Fervidibacter sacchari sac’cha.ri. N.L. gen. n. sacchari, of sugar Hyperthermophilic, microaerophilic, facultatively anaerobic, and grows chemoheterotrophically on monosaccharides and polysaccharides. Cells are ovoid- to rod-shaped, Gram-stain negative, and are 0.9–1.3 µm in width and 1.6–3.6 µm in length. Grows between 65 and 87.5 °C and an optimum temperature of 80 °C, and a pH range of 6.5–8.6 with an optimum pH of 7.5. Grows at an optimum O2 concentration of 5–10%. Grows on D-arabinose, D-galactose, D-glucose, D-rhamnose, D-ribose, D-xylose, chondroitin sulfate, colloidal chitin, galactan, gellan gum, guar gum, karaya gum, locust bean gum, xantham gum, xyloglucan, β-glucan, glycogen, starch, AFEX-pretreated corn stover, miscanthus, sugarcane bagasse, acetate and casamino acids. Grows weakly on xyloglucan under fermentation conditions. The major fatty acids (>10%) are C16:0, C18:0 and/or cyclo-C17:0, and iso-C16:0. The major respiratory quinones (>10%) are MK-8 and MK-9. The isolate and genomes of the species have been recovered from geothermal springs in the Great Basin, Nevada, USA. GC content of genomes range between 51–52%. Subunits for both the high-affinity and low-affinity terminal oxidases are encoded in the genomes. Genomes also encode a Group 3d [NiFe] hydrogenase, which produces hydrogen as an electron sink for NAD+ regeneration. The type strain PD1T (= JCM 39283T = DSM 113467T) was isolated from Great Boiling Spring in Nevada, USA. Family Fervidibacteraceae Fer.vi.di.bac.te.ra’ce.ae. N.L. masc. n. Fervidibacter type genus of the family; L. suff. -aceae ending to denote a family; N.L. fem. pl. n. Fervidibacteraceae the family of the genus Fervidibacter Thermophilic or hyperthermophilic inhabitants of freshwater thermal environments. All members are likely polysaccharide-degrading chemoheterotrophs with numerous carbohydrate-active enzymes encoded in their genomes. Aerobic, with high-affinity and/or low-affinity terminal oxidases present in the genomes. The oxidative pentose phosphate pathway and the tricarboxylic acid cycle are complete in genomes belonging to the family. The family is a distinct phylogenetic lineage in the order Fervidibacterales and the class Fervidibacteria in the phylum Armatimonadota. The type genus is Fervidibacter. Order Fervidibacterales Fer.vi.di.bac.te.ra’les. N.L. masc. n. Fervidibacter type genus of the order; L. suff. -ales ending to denote an order; N.L. fem. pl. n. Fervidibacterales the order of the genus Fervidibacter Thermophilic or hyperthermophilic inhabitants of freshwater thermal environments. All members are likely polysaccharide-degrading chemoheterotrophs with numerous carbohydrate-active enzymes encoded in their genomes. Aerobic or strictly anaerobic. Phylogenomic placement of this lineage within the Fervidibacteria and relative evolutionary divergence supports delineation of this lineage as an order within the class Fervidibacteria and phylum Armatimonadota. The type genus is Fervidibacter. Class Fervidibacteria Fer.vi.di.bac.te’ri.a. N.L. masc. n. Fervidibacter type genus of the type order of the class; L. suff. -ia ending to denote a class; N.L. neut. pl. n. Fervidibacteria the class of the order Fervidibacterales Thermophilic or hyperthermophilic inhabitants of freshwater thermal environments. All members are likely polysaccharide-degrading chemoheterotrophs with numerous carbohydrate-active enzymes encoded in their genomes. Aerobic or strictly anaerobic. Phylogenomic placement of this lineage within the Armatimonadota and relative evolutionary divergence supports delineation of this lineage as a class within the Armatimonadota. The type genus is Fervidibacter. The error has not been corrected in the PDF or HTML versions of the Article.

Nou, Nancy O↗
NASA NTRS2010

Detailed Structure of the Tropical Upper Troposphere and Lower Stratosphere as Revealed by Balloon Sonde Observations of Water Vapor, Ozone, Temperature, and Winds During the NASA TCSP and TC4 Campaigns

We report on balloon sonde measurements of water vapor and ozone using the cryogenic frost point hygrometer and electrochemical concentration cell ozonesondes made at Alajuela, Costa Rica (10.0 N, 84.2 W) during two NASA airborne campaigns: the Tropical Convective Systems and Processes (TCSP) mission in July 2005 and the Tropical Composition, Clouds, and Climate Coupling Experiment (TC4), July - August 2007. In both campaigns we found an upper troposphere that was frequently supersaturated but no evidence that deep convection had reached the tropopause. The balloon sondes were complemented by campaigns of 4 times daily high-resolution radiosondes from mid-June through mid-August in both years. The radiosonde data reveal vertically propagating equatorial waves that caused a large increase in the variability of temperature in the tropical tropopause layer (TTL). These waves episodically produced cold point tropopauses (CPTs) above 18 km, yet in neither campaign was saturation observed above approx 380 K or 17 km. The averages of the water vapor minima below this level were 5.2 ppmv in TCSP and 4.8 ppmv in TC4, and the individual profile minima all lay at or above approx 360 K. The average minima in this 360 C380 K layer provide a better estimate of the effective stratospheric entry value than the average mixing ratio at the CPT. We refer to this upper portion of the TTL as the tropopause saturation layer and consider it to be the locus of the final dehydration of nascent stratospheric air. As such, it is the local equivalent to the tape head of the water vapor tape recorder.

Selkirk, Henry B.↗
DOE OSTI2022

Metastability and Degradation of CdTe Solar Cells Investigated by nm-Scale Electrical Potential Imaging

We report on investigations of reversible metastability and irreversible degradation of MZO/CdSeTe/CdTe devices from the perspective of electric field across the device using Kelvin probe force microscopy (KPFM). The device showed reversible transitions between the light-soak state (LSS) with the best device efficiency and the dark-soak state (DSS) with an inferior efficiency. However, it showed an irreversible degradation state (DgS) driven by long-hour light soaking at an elevated temperature. The nm-scale KPFM electric field imaging on cross-sections of the devices revealed different anomalous electric field profiles. The electric field at the LSS exhibits a main peak inside the CdSeTe layer but not at the MZO/CdSeTe heterointerface, demonstrating that working junction of the device is a buried homojunction (BHJ). At the DSS, a second electric field peak was observed at the MZO/CdSeTe interface with a similar strength to the main BHJ, which probably caused the decrease in fill factor at the DSS. At the DgS, the electric field peak at the MZO/CdSeTe interface increased significantly and a third electric field was measured at the back contact of the device. Device modeling using COMSOL and in alignment with both the electric field and device current-voltage curves suggest that a slightly low n-doped CdSeTe in the region near the MZO/CdSeTe interface caused the BHJ, and that either a loss of MZO doping or increase of the conduction band offset spike due to long-term stress caused the fill factor-dominated degradation and the increased electric field near the MZO/CdSeTe interface at the DgS. The former, MZO doping decrease, is more plausible by referring the related literature.

BHJ↗
NASA NTRS2013

Solar Array Structures for 300 kW-Class Spacecraft

State-of-the-art solar arrays for spacecraft provide on the order of 20 kW of electrical power, and they usually consist of 3J solar cells bonded to hinged rigid panels about 1 inch in thickness. This structural construction allows specific mass and packaging volumes of up to approximately 70 W/kg and 15 kW/m3 to be achieved. Significant advances in solar array structures are required for future very-high-power spacecraft (300+ kW), such as those proposed for pre-positioning heavy cargo on or near the Moon, Mars, or asteroids using solar electric propulsion. These applications will require considerable increases in both W/kg and kW/m3, and will undoubtedly require the use of flexible-substrate designs. This presentation summarizes work sponsored by NASA's Game Changing Development Program since Oct. 2011 to address the challenge of developing 300+ kW solar arrays. The work is primarily being done at NASA Langley, NASA Glenn, and two contractor teams (ATK and DSS), with technical collaboration from AFRL/Kirtland. The near-tem objective of the project is design, analysis, and testing of 30-50 kW solar array designs that are extensible to the far-term objective of 300+ kW. The work is currently focused on three designs: the MegaFlex concept by ATK, the Mega-ROSA concept by DSS, and an in-house 300-kW Government Reference Array concept. Each of these designs will be described in the presentation. Results obtained to date by the team, as well as future work plans, for the design, analysis, and testing of these large solar array structures will be summarized.

Pappa, Richard↗
DOE OSTI2020

A High Precision Measurement of the Proton Charge Radius at JLab

The elastic electron-proton (e-p) scattering and the spectroscopy of hydrogen atoms are the two traditional methods to determine the proton charge radius (r p ). In 2010, a new method using muonic hydrogen (µH) 1 spectroscopy reported a r p result that was nearly ten times more precise but significantly smaller than the values from the compilation of all previous r p measurements. This discrepancy is often referred to as the "proton charge radius puzzle". In order to investigate the puzzle, the PRad experiment (E12-11-106) was first proposed in 2011 and performed in 2016 in Hall B at the Thomas Jefferson National Accelerator Facility, with both 1.1 and 2.2 GeV electron beams. The experiment measured the e-p elastic scattering cross sections in an unprecedented low values of momentum transfer squared region (Q 2 = 2.1× 10 -4 -0.06 (GeV/c) 2 ), with a sub-percent precision. The PRad experiment utilized a calorimetric method that was magnetic-spectrometer-free. Its detector setup included a large acceptance and high resolution calorimeter (HyCal), and two large-area, high spatial-resolution Gas Electron Multiplier (GEM) detectors. To have a better control over the systematic uncertainties, the absolute e-p elastic scattering cross section was normalized to that of the well-known Møller scattering process, which was measured simultaneously during the experiment. For each beam energy, all data with different Q 2 were collected simultaneously with the same detector setup, therefore sharing the same integrated luminosity. The windowless H 2 gas-flow target utilized in the experiment largely removed a typical background source, the target cell windows. The proton charge radius was determined as r p = 0.831±0.007 stat. ±0.012 p fm, which is smaller than the average r p from previous e-p elastic scattering experiments, but in agreement with the µH spectroscopic results within the experimental uncertainties.

Source record↗
DOE OSTI2022

High-pressure Cr 3+ R-line luminescence of zoisite and kyanite: a probe of octahedral site distortion

Here, the Cr 3+ luminescence spectra of zoisite and kyanite, two geologically important minerals, were studied up to 40 and 20 GPa, respectively, in various pressure media. Cr 3+ substitutes into the octahedral aluminum sites in both minerals and the R-line luminescence is a particularly sensitive site-specific probe of the octahedral Al site. Unlike many previous studies where Cr 3+ luminescence was utilized, both these minerals have multiple highly distorted octahedral sites resulting in very large splitting of their R-lines, ~ 300 cm -1 in zoisite and ~ 360 cm -1 in kyanite (for reference, ruby is 29 cm -1 ). For zoisite, the R-line splitting increases as pressure increases and more than triples from its ambient value by 40 GPa, while the R-line splitting in kyanite from the M1 and M2 sites does not change when compressed in a Ne pressure medium up to 20 GPa. We do not observe evidence of any phase transitions in either zoisite or kyanite across the pressure range of these new luminescence measurements. Furthermore, we present some high-pressure luminescence results where kyanite was known to be bridged between the diamond anvils and show how these spectra illustrate the different effect of uniaxial relative to hydrostatic stress on luminescence spectra.

36 MATERIALS SCIENCE↗
DOE OSTI2026

Harnessing Heterologous Bacterial Two-Component Systems as Biosensors to Address Challenges in Fermentation Scale-Up

Scaling up bacterial fermentation from bench to industrial scale often results in unpredictable performance losses, possibly in part due to changes in microenvironmental conditions such as pH. To investigate this, we developed a suite of pH-sensitive biosensors from bacterial two-component systems (TCSs) that provide a dynamic, fluorescent readout in response to extracellular pH changes. TCSs consist of a periplasmic sensor histidine kinase (HK) that, in response to an extracellular stimulus, autophosphorylates intracellularly and subsequently transfers the phosphate to a cognate response regulator (RR) that modulates transcription of target genes. We utilized three pH-responsive TCSs (referred to here as CVJ1, CVJ30, and CVJ79) and linked their output to GFP. This was achieved by placing the RR promoter upstream of GFP or by constructing a chimeric RR composed of the native receiver domain and the DNA-binding domain of another well-characterized RR with a defined promoter. All components - HK, RR (native or chimeric), and GFP under its corresponding promoter - were cloned into a broad-host-range plasmid. Sensors were validated in Escherichia coli and Pseudomonas putida, including the muconic acid-producing strain P. putida TL207. All three biosensors successfully reported pH, with fluorescence (normalized to optical density) correlating strongly with media pH. Among the native sensors, CVJ79 showed the most robust performance while CVJ1 also performed best in its native form; CVJ30 exhibited improved functionality as a chimera, suggesting that modular RR design can enhance compatibility in some heterologous hosts. Further, CVJ79 was activated by alkaline conditions, while CVJ30 responded to acidic environments. Notably, CVJ1 was induced by high pH in wild-type E. coli and P. putida, but low pH in TL207. The observed differences in sensor activation between strains - particularly the divergent response of CVJ1 - suggest that host-specific regulatory pathways may influence how cells perceive and adapt to pH stress. Moving forward, these biosensors can be used to guide the rational design of more robust strains, optimize process conditions in real time, and inform strategies to minimize physiological heterogeneity during scale-up. Integrating these tools into high-throughput screening and bioreactors will be a key step toward improving predictability and performance in industrial bioprocesses.

09 BIOMASS FUELS↗
DOE OSTI2025

High-Spectral Resolution Lidar (HSRL) Instrument Handbook

High-spectral-resolution lidar (HSRL) systems provide vertical profiles of optical depth, backscatter cross-section, depolarization, and backscatter phase function. All HSRL measurements are calibrated by reference to molecular scattering, which is measured at each point in the lidar profile. Like the Raman lidar (RL), but unlike simple backscatter lidars such as the micropulse lidar (MPL), this enables the HSRL to measure backscatter cross-sections and optical depths without prior assumptions about the scattering properties of the atmosphere. The depolarization observations allow robust discrimination between ice and water clouds. Rigorous error estimates can be computed for all measurements. A very narrow angular field of view reduces multiple scattering contributions. The small field of view, coupled with a narrow optical bandwidth, nearly eliminates noise due to scattered sunlight. The laser transmitter is a diode-pumped, frequency-doubled Nd:YAG laser. Narrow-band, single-frequency operation is provided by injection seeding with a single-frequency, cw-diode-pumped diode laser. The main laser cavity is maintained in resonance with the seed laser by adjusting the cavity length to minimize the time between the Q-switch trigger and the emission of the laser pulse. The emission wavelength is tuned via temperature control of the seed laser crystal and is locked to line #1109 of the iodine absorption spectra. Locking is accomplished by minimizing the transmission through a 2-cm-long iodine absorption cell. Use of a high-repetition-rate laser and expansion of the transmitted beam through a 400-mm telescope reduces the transmitted energy density to eye-safe levels. It is possible to look directly into the output beam without hazard. The receiver and transmitter use the same afocal telescope, simplifying the maintenance of stable alignment of the transmitter and receiver although the angular FOV is only 100 μrad. The small FOV and the 4-kHz repetition rate also limit the near-field signal strength, making it possible to record continuous profiles that start at an altitude of ~100 m and extend to 30 km using photon counting detectors. The small FOV also suppresses multiple scattering contributions.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2024

Non-linear MHD modelling of transients in tokamaks: a review of recent advances with the JOREK code

Transient magneto-hydrodynamic (MHD) events like edge localized modes (ELMs) or disruptions are a concern for magnetic confinement fusion power plants. Research with the MHD code JOREK towards understanding control of such instabilities is reviewed here in a concise way to provide a complete overview, while we refer to the original publications for details. Experimental validation for unmitigated vertical displacement events progressed. The mechanism of vertical force mitigation by impurity injection was identified. Two-way eddy current coupling to CARIDDI was completed. Shattered pellet injection was simulated in JET, KSTAR, ASDEX Upgrade (AUG) and ITER. Benign runaway electron beam termination in JET and ITER was studied. Coupling of kinetic REs to the MHD is ongoing and a virtual RE synchrotron radiation diagnostic was developed. Regarding pedestal physics, regimes devoid of large ELMs in AUG were simulated and predictive JT60-SA simulations are ongoing. For ELM suppression by resonant magnetic perturbations (RMPs), AUG, ITER and EAST simulations were performed. A free boundary RMP model was validated against experiments. Evidence for penetrated magnetic islands at the pedestal top based on AUG experiments and simulations was found. Simulations of the naturally ELM-free quiescent H-mode in AUG and HL-3 show external kink mode formation prevents pedestal build-up towards an ELM within windows of the edge safety factor. With kinetic neutral particles, high field side high density formation in ITER was simulated and with kinetic impurities, tungsten transport in AUG RMP plasmas was studied. To capture turbulent transport, electro-static full-f particle in cell models for ion temperature gradient and trapped electron modes were established and benchmarked. Application to RMP plasmas shows enhanced turbulence in comparison to unperturbed states. Energetic particle interactions with MHD were studied. Flux pumping that prevents the safety factor on axis from dropping below unity was simulated. First non-linear stellarator applications include current relaxation in $l$ = 2 stellarators, while verification for advanced stellarators progresses.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗
NASA NTRSDate not supplied

NASA's Habitation Development Status: Current Concepts and ISRU Opportunities

Introduction: The National Aeronautics and Space Administration (NASA) is embarking on a bold journey to return humankind to the Moon and onward to Mars with innovative commercial, international, and academic partnerships. Under the Artemis series of missions, NASA seeks to establish sustained human exploration of deep space through an objectives-based approach. This approach drives the identification of needed system functionality and the current and future capabilities which will eventually allow humanity to sustainably live beyond Earth. Providing evolvable and scalable habitation is a cornerstone function that calls for the collection and integration of current, developmental, and future technologies that can meet near-term exploration needs while growing into long-term sustained presence. NASA is advancing in-space habitation through its Next Space Technologies for Exploration Partnerships (NextSTEP) model while designing lunar, Mars transit, and Mars surface habitat government reference concepts for Artemis missions. These efforts have unveiled possible near-term opportunities for the in-space resource utilization community if human habitation is considered a future customer of space resources. NextSTEP Habitation Development: NASA is closely working with commercial partners under its NextSTEP Appendix A model to advance habitation systems in the arena of inflatable and composite habitation structures. Such efforts promise efficiencies in volumetric packaging and overall spacecraft mass respectively. Recent testing by commercial partners have helped to quantify possible failure mechanisms for inflatable structures while advancing their technology towards eventual flight certification. The advancement of such Class II habitation structures, in which the habitat is only fully deployed once in-space or on a planetary surface, is critical to providing increased habitable volume for long-duration missions with no additional mass penalties. The progression of such technology is infused into NASA’s government reference concepts for notional deep space habitation concepts. Current Government Reference Concepts: To best inform the formulation of future collaborative solicitations, NASA employs the practice of internally developing reference concepts for future exploration elements. These concepts aid in identifying the functions and capabilities needed to complete NASA missions as well as feasible solutions within the timeframe needed. Government reference concepts for a lunar Surface Habitat, Mars Transit Habitat, and Mars Sur-face Habitat are continuously being developed and updated to better guide the Agency’s overall exploration architecture. Lunar Surface Habitat. As NASA returns to the Moon, it is evaluating possible lunar surface habitation concepts. The Surface Habitat (SH) reference concept entails a hybrid metallic-inflatable structure capable of initially housing two crew for surface stays of up to 30 days in duration. While initial missions may span ~7 days in duration, consideration is being given to expanding SH’s capability to support a crew of four for up to 60 days over its 15-year design life. Functionally, the SH serves as a ‘hub’ for all Artemis crewed surface operations, providing internal volume for maintenance, medical, logistics, science utilization, and extravehicular activity (EVA) support in addition to core habitation functionality such as environmental control and life support (ECLS) and power generation and distribution among many others. Additionally, NASA has entered a study agreement with the Italian Space Agency (Agenzia Spaziale Italiana – ASI) to investigation a possible Multi-Purpose Habitat (MPH) as an additional or augmenting habit-able element for the lunar surface. Mars Transit Habitat. NASA’s current architectural concept for initial human missions to Mars entails the utilization of a transit habitat (TH) to transport a four-person crew to and from Mars orbit, departing from and returning to a lunar near-rectilinear halo orbit (NRHO), over the course of a ~1,200-day mission. While holding a similar 15-year design lifetime, TH will also support a series of analog mission activities in NRHO to gradually test the systems and interaction with lunar surface elements, some of which may be adapted for Mars surface exploration. Holding similar functional capabilities as SH, TH is sized to support much longer durations in space and greater logistical independence. Mars Surface Habitat. NASA is still exploring the concept of operations for initial crewed missions to Mars. As such, the Mars Surface Habitat (MSH) concept is still in its infancy as options for mobile, pressurized habitation and stationary habitats are being explored. It is expected MSH will leverage heavily from the lunar SH and possible lunar pressurized rover, however the very different Martian environment will likely necessitate modifications. ISRU Opportunities: Despite the advancements under NASA’s NextSTEP habitation work and continually optimized reference concepts, NASA is facing near-term mass and power challenges that may create opportunities to the ISRU community by providing yet another possible customer for space resources. While NASA desires to use regenerative ECLS systems (ECLSS) for all habitation concepts, their operation comes with initial mass penalties and maintenance overheads when compared to simpler open-loop architectures. Because of this, NASA is currently pro-posing an open-loop, consumables-based architecture for its surface habitats to achieve initial launch and delivery lander mass targets while scarring for the in-corporation of regenerative ECLSS to meet longer mission durations and sustained presence. With such an architecture, oxygen and potable water are needed consumables, which initial ISRU systems may be able to provide in a pilot capacity. Although the TH is expecting to utilize regenerative ECLSS, advancements in lunar surface-based ISRU and possible re-supply of spacecraft in lunar or Mars orbit could significantly reduce the logistical need for ECLSS related spares on TH and possibly allow a similar open-loop and consumables-based architecture. In addition to ECLSS mass concerns, the SH is facing challenges with energy storage to support operations over periods of darkness exceeding 100 hours. Both battery and fuel cell-based power architectures are being traded, opening options for external power generation and energy storage. One possibility is ISRU-produced H2 and O2 feeding external primary fuel cell systems which could supplement habitation power generation while reducing initial mass and volume until much more powerful fission power systems might be deployed. Conclusion: Significant advancements are being made in evolvable habitation concepts that span from near-term technologies, such as inflatable structures under NextSTEP, to potentially revolutionary capabilities like the lunar surface construction as funded through the Moon-to-Mars Planetary Autonomous Construction Technologies (MMPACT) project. As NASA investigates both heritage capabilities and rap-idly advancing, disruptive technologies, there are likely many near-term opportunities for initial ISRU capabilities to significantly aid human habitation and increase self-sufficiency beyond Earth.

habitation↗
DOE OSTI2026

Facilitating Data Collection of Maintenance Events to Populate the Hydrogen Component Reliability Database (HyCReD)

The Hydrogen Component Reliability Database (HyCReD) is a collaborative project between the National Renewable Energy Laboratory, the University of Maryland, and hydrogen stakeholders to improve safety and reliability for hydrogen facilities by implementing component reliability data taxonomies that support hydrogen infrastructure failure rate analysis. The project aims to quantify failure rates of hydrogen components through high-quality data collection and analysis on root causes and maintenance needed. HyCReD provides a common database for cataloging hydrogen component failures which exists for reliability research in many other mature industries [2]. The database fills a gap for the hydrogen community by providing a scientifically rigorous approach to quantitative risk assessment (QRA), prognostic health management (PHM), and reliability-centered maintenance (RCM) analysis. High level results will be aggregated and anonymized to protect company sensitive information; detailed results will be used to help address issues of hydrogen components. These advanced analytics will support accelerated deployment of hydrogen infrastructure by enabling better: design and safety of projects (safety codes and standards development), infrastructure reliability and cost (component failure rates, maintenance protocols), and component R&D needs (robust supply chain). A key to a successful HyCReD implementation is facilitating the ease of reporting and data quality in the database that can be used for analysis. Maintenance data was a previously identified gap in initial efforts to populate and validate the database taxonomies [3]. Collection of maintenance data will be instrumental in identifying failure modes and rates, identifying incipient component failures or reduced performance, cataloging best practices for maintenance routines and methods for prognostic health management, and quantifying the risk and effect of different failure modes. Several key priorities are identified for streamlined data collection to achieve quality and detailed failure data: Applicability, Ease of Use, Accessibility, and Information Security. The HyCReD team has now begun deployment of the database to several companies and groups that have signed non-disclosure agreements to facilitate the data collection of failures in industry hydrogen refueling station infrastructure. This paper will provide an update into the process of HyCReD deployment including the development of a coding guide for facility personnel to reference and ensure data quality and consistency from one station to another as well as implementation of contextually dependent data fields of system taxonomy and formatted entries to provide ease of use. The goal is to communicate the lessons learned from the roll-out to technicians and engineers in the field, and the addition of need for high level of security to protect all stakeholders.

29 ENERGY PLANNING, POLICY, AND ECONOMY↗
DOE OSTI2024

Magneto-Stokes flow in a shallow free-surface annulus

In this study, we analyse ‘magneto-Stokes’ flow, a fundamental magnetohydrodynamic (MHD) flow that shares the cylindrical-annular geometry of the Taylor–Couette cell but uses applied electromagnetic forces to circulate a free-surface layer of electrolyte at low Reynolds numbers. The first complete, analytical solution for time-dependent magneto-Stokes flow is presented and validated with coupled laboratory and numerical experiments. Three regimes are distinguished (shallow-layer, transitional and deep-layer flow regimes), and their influence on the efficiency of microscale mixing is clarified. The solution in the shallow-layer limit belongs to a newly identified class of MHD potential flows, and thus induces mixing without the aid of axial vorticity. We show that these shallow-layer magneto-Stokes flows can still augment mixing in distinct Taylor dispersion and advection-dominated mixing regimes. The existence of enhanced mixing across all three distinguished flow regimes is predicted by asymptotic scaling laws and supported by three-dimensional numerical simulations. Mixing enhancement is initiated with the least electromagnetic forcing in channels with order-unity depth-to-gap-width ratios. If the strength of the electromagnetic forcing is not a constraint, then shallow-layer flows can still yield the shortest mixing times in the advection-dominated limit. Our robust description of momentum evolution and mixing of passive tracers makes the annular magneto-Stokes system fit for use as an MHD reference flow.

58 GEOSCIENCES↗
NASA NTRS2000

Bioelectrical impedance analysis. What does it measure?

Bioelectrical impedance analysis (BIA) has been proposed for measuring fat-free mass, total body water, percent fat, body cell mass, intracellular water, and extracellular water: a veritable laboratory in a box. Although it is unlikely that BIA is quite this versatile, correlations have been demonstrated between BIA and all of these body compartments. At the same time, it is known that all of the compartments are correlated among themselves. Because of this, it is difficult to determine whether BIA is specific for any or all of these compartments. To investigate this question, we induced acute changes in total body water and its compartments over a 3-h period. Using this approach, we demonstrated that multifrequency BIA, using the Cole-Cole model to calculate the zero frequency and infinite frequency resistance, measures extracellular and intracellular water.

NASA Discipline Regulatory Physiology↗
DOE OSTI2025

Determination of a suitable molar absorption coefficient (ε) for lignin analysis of fibrous plants using the CASA method

In this study, the benchmarking and applicability of the CASA method for the analysis of fibrous plants, such as flax, hemp, and jute, is evaluated. Lignin is a phenolic biopolymer present in plant cell walls and is composed of three primary monomeric units, designated G, S, and H. Various factors, such as the genetic variability, influence the relative proportions of these units in plant samples. Recently, the cysteine-assisted sulfuric acid (CASA) method has been introduced as a rapid method for the quantification of lignin in wood samples. The aim of this study is to establish a suitable molar absorption coefficient (ε, L·g –1 ·cm –1 ) to adapt the CASA method for use with annual plant fibers. This investigation was motivated by the technical advantages of the CASA method, including higher throughput, lower reaction temperatures, and ecological benefits due to the absence of carcinogenic, mutagenic, or reprotoxic (CMR) substances and the need for minimal sample quantities. In this method, lignin solubilization is facilitated by cysteine, which is an amino acid that enhances the reaction kinetics, using a one-hour incubation period. However, as with any spectrophotometric technique, the CASA method depends on a molar absorption coefficient (ε) that varies according to the ratio of the aromatic units within the polymer. To evaluate the suitability of CASA for quantifying lignin in economically significant plant fibers, we investigate the impact of the unit ratios on the accuracy of ε. The results are compared with those of two widely recognized lignin analytical methods: the Klason method, which is a gravimetric reference method, and the acetyl bromide soluble lignin method, which is the most commonly used spectrophotometric approach. The final ε obtained in this study reveals a relative difference in lignin content ranging from –8 % to +9 % based on a comparison between the CASA and Klason methods across different industrial hemp varieties. As a result, using known G:S ratios in annual fibrous plants, ε can be estimated from our results.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2022

Holographic Plasma Lenses

We report a hologram fully encodes a three-dimensional light field by imprinting the interference between the field and a reference beam in a recording medium. Here we show that two collinear pump lasers with different foci overlapped in a gas jet produce a holographic plasma lens capable of focusing or collimating a probe laser at intensities several orders-of-magnitude higher than the limits of a nonionized optic. We outline the theory of these diffractive plasma lenses and present simulations for two plasma mechanisms that allow their construction: spatially varying ionization and ponderomotively driven ion-density fluctuations. Damage-resistant plasma optics are necessary for manipulating high-intensity light, and divergence control of high-intensity pulses—provided by holographic plasma lenses—will be a critical component of high-power plasma-based lasers.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗