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At least 1,009 records · Page 56

Researching Seeds: Films, Sanitation Methods, Microbiological Growth, Viability, and Selection for New Crops

A major factor in long-term human exploration of the solar system is crop growth in microgravity. Space crops can provide fresh, nutritious food to supplement diets for astronauts. Important factors impacting space plant growth and consumption are water delivery to root zone in microgravity, sanitation methods for microbiological safety, plant responses to light quality/spectrum, and identifying optimal edible plants suitable for growth on the International Space Station (ISS). Astronauts growing their own food on the ISS provides necessary data for crop production for long duration deep space missions. The seed film project can be used in Advanced Plant Habitat and Veggies that are currently being utilized on the ISS.

Plant Biology↗

Increasing the Statistical Rigor of Cross-Species Differential Expression Analysis

Microgravity inflicts substantial, but undercharacterized, pressure on organisms that induces metabolic responses such as increased microbial virulence and antibiotic resistance, altered organ weights in developing rats, and loss of bone tissue in astronauts. Numerous studies have analyzed the effects of microgravity on specific organisms, tissues, or test conditions, but these projects are necessarily limited by the small sample size of space research. Increasing the sample size of spaceflight studies is non-trivial; however, pooling data from numerous studies can greatly increase the statistical rigor of comparative analyses. The GeneLab houses datasets from 73 spaceflight studies that performed transcription profiling assays. These data encompass a diverse array of organisms ranging from Escherichia coli to Mus musculus to Homo sapiens and comprise studies analyzing ionizing radiation, mammalian pregnancy, etc. Collectively, the GeneLab database contains a large quantity of transcription assays and RNA sequence data analyzing Differential Gene Expression (DGE) between microand normogravity. Xspecies, a cross-species analysis method for DGE developed by Kristiansson, et al. in 2012, identifies homologous genes between species that are universally up- or downregulated in response to test conditions. Previous work by an intern at GeneLab applied Xspecies to 19 datasets containing seven different species and identified 14 homologous groups differentially expressed under spaceflight conditions including several heat shock proteins and cytoskeletal components. Unfortunately, these results may be biased by the disproportionate number of studies on Arabidopsis thaliana (5) and Mus musculus (6) and the results are not normalized by evolutionary distances. Here, we present modifications to the Xspecies algorithm that permits incorporation of multi-omic data and normalizes data for effect size, directionality, and evolutionary distances. We then apply this algorithm to all currently available GeneLab studies

Xspecies↗

Plans and Recent Developments for Fluid Physics Experiments Aboard the ISS

From the very first days of human spaceflight, NASA has been conducting experiments in space to understand the effect of weightlessness on physical and chemically reacting systems. NASA Glenn Research Center (GRC) in Cleveland, Ohio has been at the forefront of this research looking at both fundamental studies in microgravity as well as experiments targeted at reducing the risks to long duration human missions to the moon, Mars, and beyond. In the current International Space Station (ISS) era, we now have an orbiting laboratory that provides the highly desired condition of long-duration microgravity. This allows continuous and interactive research similar to Earth-based laboratories. Because of these capabilities, the ISS is an indispensable laboratory for low gravity research. NASA GRC has been actively involved in developing and operating facilities and experiments on the ISS since the beginning of a permanent human presence on November 2, 2000. As the lead Center for Fluid Physics, NASA GRC is developing and testing the Pack Bed Reactor Experiment (PBRE), Zero Boil Off (ZBOT) Two Phase Flow Separator Experiment (TPFSE), Multiphase Flow Heat Transfer (MFHT) Experiment and the Electro-HydroDynamic (EHD) experiment. An overview each experiment, including its objectives, concept and status will be presented. In addition, data will be made available after a nominal period to NASAs Physical Science Informatics PSI database to the scientific community to enable additional analyses of results.

Two phase flow↗

Acquisition of Long-Duration, Low-Gravity Slosh Data Utilizing Existing ISS Equipment (SPHERES) for Calibration of CFD Models of Coupled Fluid-Vehicle Behavior

Accurate prediction of coupled fluid slosh and launch vehicle or spacecraft dynamics (e.g., nutation/precessional movement about various axes, attitude changes, ect.) requires Computational Fluid Dynamics (CFD) models calibrated with low-gravity, long duration slosh data. Recently completed investigations of reduced gravity slosh behavior have demonstrated the limitations of utilizing parabolic flights on specialized aircraft with respect to the specific objectives of the experiments. Although valuable data was collected, the benefits of longer duration low-gravity environments were clearly established. The proposed research provides the first data set from long duration tests in zero gravity that can be directly used to benchmark CFD models, including the interaction between the sloshing fluid and the tank/vehicle dynamics. To explore the coupling of liquid slosh with the motion of an unconstrained tank in microgravity, NASA's Kennedy Space Center, Launch Services Program has teamed up with the Florida Institute of Technology (FIT), Massachusetts Institute of Technology (MIT) and the Office of the Chief Technologist (OCT) to perform a series of slosh dynamics experiments on the International Space Station using the SPHERES platform. The Synchronized Position Hold Engage Reorient Experimental Satellites (SPHERES) testbed provides a unique, free-floating instrumented platform on ISS that can be utilized in a manner that would solve many of the limitations of the current knowledge related to propellant slosh dynamics on launch vehicle and spacecraft fuel tanks. The six degree of freedom (6-DOF) motion of the SPHERES free-flyer is controlled by an array of cold-flow C02 thrusters, supplied from a built-in liquid C02 tank. These SPHERES can independently navigate and re-orient themselves within the ISS. The intent of this project is to design an externally mounted tank to be driven inside the ISS by a set of two SPHERES devices (Figure 1 ). The tank geometry simulates a launch vehicle upper stage propellant tank and the maneuvers replicate those of real vehicles. The design includes inertial sensors, data acquisition, image capture and data storage interfaces to the SPHERES VERTIGO computer system on board the flight article assembly. The design also includes mechanical and electronic interfaces to the existing SPHERES hardware, which include self-contained packages that can operate in conjunction with the existing SPHERES electronics.

Schallhorn, Paul↗

Acquisition of Long-Duration, Low-Gravity Slosh Data Utilizing Existing ISS Equipment (SPHERES) for Calibration of CFD Models of Coupled Fluid-Vehicle Behavior

Accurate prediction of coupled fluid slosh and launch vehicle or spacecraft dynamics (e.g., nutation/precessional movement about various axes, attitude changes, ect.) requires Computational Fluid Dynamics (CFD) models calibrated with low-gravity, long duration slosh data. Recently completed investigations of reduced gravity slosh behavior have demonstrated the limitations of utilizing parabolic flights on specialized aircraft with respect to the specific objectives of the experiments. Although valuable data was collected, the benefits of longer duration low-gravity environments were clearly established. The proposed research provides the first data set from long duration tests in zero gravity that can be directly used to benchmark CFD models, including the interaction between the sloshing fluid and the tank/vehicle dynamics. To explore the coupling of liquid slosh with the motion of an unconstrained tank in microgravity, NASA's Kennedy Space Center, Launch Services Program has teamed up with the Florida Institute of Technology (FIT), Massachusetts Institute of Technology (MIT) and the NASA Game Changing Development Program (GCD) to perform a series of slosh dynamics experiments on the International Space Station using the SPHERES platform. The Synchronized Position Hold Engage Reorient Experimental Satellites (SPHERES) testbed provides a unique, free-floating instrumented platform on ISS that can be utilized in a manner that would solve many of the limitations of the current knowledge related to propellant slosh dynamics on launch vehicle and spacecraft fuel tanks. The six degree of freedom (6-DOF) motion of the SPHERES free-flyer is controlled by an array of cold-flow C02 thrusters, supplied from a built-in liquid C02 tank. These SPHERES can independently navigate and re-orient themselves within the ISS. The intent of this project is to design an externally mounted tank to be driven inside the ISS by a set of two SPHERES devices (Figure 1). The tank geometry simulates a launch vehicle upper stage propellant tank and the maneuvers replicate those of real vehicles. The design includes inertial sensors, data acquisition, image capture and data storage interfaces to the SPHERES VERTIGO computer system on board the flight article assembly. The design also includes mechanical and electronic interfaces to the existing SPHERES hardware, which include self-contained packages that can operate in conjunction with the existing SPHERES electronics

Schallhorn, Paul↗

Laboratory and theoretical models of planetary-scale instabilities and waves

The continuous low-g environment of the orbiting space shuttle provided a setting for conducting geophysical fluid model experiments with a completely consistent representation of sphericity and the resultant radial gravity found on astrogeophysical objects. This is possible because in zero gravity an experiment can be constructed that has its own radial buoyancy forces. The dielectric forces in a liquid, which are linearly dependent on fluid temperature, give rise to an effectively radial buoyancy force when a radial electrostatic field is applied. The Geophysical Fluid Flow Cell (GFFC) experiment is an implementation of this idea in which fluid is contained between two rotating hemispheres that are differentially heated and stressed with a large ac voltage. The GFFC flew on Spacelab 3 in May 1985. Data in the form of global Schlieren images of convective patterns were obtained for a large variety of configurations. These included situations of rapid rotation (large Taylor numbers), low rotation, large and small thermal forcing, and situations with applied meridional temperature gradients. The analysis and interpretation of the GFFC-85 data are being conducted. Improvements were developed to the GFFC instrument that will allow for real-time (TV) display of convection data and for near-real-time interactive experiments. These experiments, on the transition to global turbulence, the breakdown of rapidly rotating convective planforms and other phenomena, are scheduled to be carried out on the International Microgravity Laboratory (IML-1) aboard the shuttle in June 1990.

Hart, John E.↗

Aerodynamic, unsteady, kinetic and heat loss effects on the dynamics and structure of weakly-burning flames

The first objective of the program is to introduce the meritorious counterflow methodology in microgravity in order to quantify the steady and unsteady characteristics of weakly-burning premixed and diffusion flames for a wide variety of conditions including elevated pressures. Subsequently, through detailed modeling and comparisons with the experimental data, to provide physical insight into the elementary mechanisms controlling the flame response. The configuration offers good control over the parameters of interest and can be modelled closely. The knowledge which will be gained from the counterflow flames will be subsequently used to analyze near-limit phenomena related to other configurations by conducting detailed numerical simulations including multidimensional ones. Among the problems to be analyzed are the downward and upward propagation of near-limit flames in tubes and phenomena observed in spherical and cylindrical geometries.

Egolfopoulos, Fokion N.↗

Flame Spread Across Liquids

The principal goal of our recent research on flame spread across liquid pools is the detailed identification of the mechanisms that control the rate and nature of flame spread when the liquid pool is initially at an isothermal bulk temperature that is below the fuel's flash point temperature. In our project, we specialize the subject to highlight the roles of buoyancy-related processes regarding the mechanisms of flame spread, an area of research cited recently by Linan and Williams as one that needs further attention and which microgravity (micro-g) experiments could help to resolve. Toward resolving the effects of buoyancy on this flame spread problem, comparisons - between 1-g and micro-g experimental observations, and between model predictions and experimental data at each of these gravitational levels - are extensively utilized. The present experimental and computational foundation is presented to support identification of the mechanisms that control flame spread in the pulsating flame spread regime for which long-duration, micro-g flame spread experiments have been conducted aboard a sounding rocket.

Ross, Howard D.↗

Third and Final Shuttle Mission of the Isothermal Dendritic Growth Experiment Conducted: Highest Supercooling Ever Recorded Achieved

Dendrites describe the treelike crystal morphology commonly assumed in metals and alloys that freeze from supercooled or supersaturated melts. There remains a high level of engineering interest in dendritic solidification because the size, shape, and orientation of the dendrites determine the final microstructure of a material. It is the microstructure that then determines the physical properties of cast or welded products. Although it is well known that dendritic growth is controlled by the transport of latent heat from the moving solid-liquid interface, an accurate and predictive model has not yet been developed. The effects of gravity-induced convection on the transfer of heat from the interface have prevented adequate testing, under terrestrial conditions, of solidification models. The Isothermal Dendritic Growth Experiment (IDGE) constituted a series of three microgravity experiments flown aboard the Space Shuttle Columbia. The apparatus was used to grow and record dendrite solidification in the absence of gravity-induced convective heat transfer, thereby producing a wealth of benchmark-quality data for testing solidification models and theories.

Glicksman, Martin E.↗

Image-Capture Devices Extend Medicine's Reach

Johnson Space Center, Henry Ford Hospital in Detroit, and Houston-based Wyle Laboratories collaborated on NASA's Advanced Diagnostic Ultrasound in Microgravity (ADUM) experiment, which developed revolutionary medical ultrasound diagnostic techniques for long-distance use. Mediphan, a Canadian company with U.S. operations in Springfield, New Jersey drew on NASA expertise to create frame-grabber and data archiving technology that enables ultrasound users with minimal training to send diagnostic-quality ultrasound images and video to medical professionals via the Internet in near real time allowing patients as varied as professional athletes, Olympians, and mountain climbers to receive medical attention as soon as it is needed.

Source record↗

A Novel Method for Characterizing Spacesuit Mobility through Metabolic Cost

Spacesuit mobility has historically been defined and characterized by a combination of range of motion and joint torque of the individual anatomical joints when performing isolated motions meant to drive that joint only in a given orthogonal plane. While this has been the standard approach for several decades, there are numerous shortcomings that suit designers and engineers would like to see rectified. First, the lack of a standardized method for collecting both range of motion and joint torque translates to many different test setups, procedures and methods of data analysis. Second, all of these previously used methods for data collection lack some degree of repeatability, even within the same test setup and the same conductor; in addition, attempts at higher fidelity data collection techniques require high overhead and cost with minimal improvement. Lastly, isolated motions in standard anatomical planes are not representative of real‐world tasks that a crewmember would be performing during an EVA, be it microgravity or surface exploration based. To address these shortcomings, options are being explored within the Space Suit and Crew Survival Systems Branch to ascertain the feasibility of an alternative approach to defining mobility - one that is more repeatable, lower overhead, and more tied to functional EVA tasks. This paper serves to document the first attempt at such an alternative option - one that looks at the metabolic energy‐cost of a spacesuit. In other words, can we objectively compare the mobility of a spacesuit by evaluating the metabolic cost of that suit to the wearer while performing a battery of functional EVA tasks?

McFarland, Shane↗

Analysis of the Quality of Parabolic Flight

Parabolic flight allows researchers to conduct several micro-gravity experiments, each with up to 20 seconds of micro-gravity, in the course of a single day. However, the quality of the flight environment can vary greatly over the course of a single parabola, thus affecting the experimental results. Researchers therefore require knowledge of the actual flight environment as a function of time. The NASA Flight Opportunities program (FO) has reviewed the acceleration data for over 400 parabolas and investigated the level of micro-gravity quality. It was discovered that a typical parabola can be segmented into multiple phases with different qualities and durations. The knowledge of the microgravity characteristics within the parabola will prove useful when planning an experiment.

Lambot, Thomas↗

Flight Readiness of Mochii S: Portable Spectroscopic Scanning Electron Microscope Facility on the International Space Station (ISS)

The ISS (International Space Station) currently lacks the capability to image and chemically analyze nano-to-micron scale particles from numerous engineering systems. To identify these particles, we must wait for a re-entry vehicle to return them from low earth orbit for ground-based SEM (Scanning Electron Microscope) / EDS (Energy Dispersive X-Ray Spectroscopy) analysis. This may take months, potentially delaying the affected system. Having an EDS-equipped SEM (Mochii S) aboard the ISS will accelerate response time thereby enhancing crew and vehicle safety by rapid and accurate identification of microscopic threats, especially in time-critical situations.The Mochii S payload will be stationed in the Japanese Experiment Module (JEM) powered by 120 VAC (Volts Alternating Current) inverter and connected to station Ethernet and WiFi (Fig. 1). To date the Mochii S payload has undergone testing for command and data handling, power quality, flight vibration, and radiation testing at Johnson Space Center (JSC). Mochii's high-RPM (Revolutions Per Minute) rotating vacuum pumps and high voltage systems have been reviewed to meet safety standards by JSC (Johnson Space Center) Engineering. Topology of the system in the JEM module has been baselined by ISS Safety and JAXA (Japan Space Exploration Agency). Digital controls to and from ISS over Joint Station LAN (Local Area Network) uplink have been simulated and the latencies and data rates have been found to be sufficient for successful operation of the payload from ground.Transporting sensitive electron optical instruments aboard a rocket that sustains 7G acceleration for 8 minutes and then operating it the unique microgravity (micro-g) environment is no trivial matter. To meet strict safety requirements and increase robustness for mission success, over 500 unique verifications must be completed before the payload is certified for spaceflight. Two of which will be discussed in detail are: vibroacoustic testing and magnetic susceptibility shielding and validation.

Mochii↗

Experiment K-6-22. Growth hormone regulation, synthesis and secretion in microgravity. Part 1: Somatotroph physiology. Part 2: Immunohistochemical analysis of hypothalamic hormones. Part 3: Plasma analysis

The objectives of the 1887 mission were: (1) to determine if the results of the SL-3 pituitary gland experiment (1) were repeatable; and (2) to determine what effect a longer mission would have on the rat pituitary gland growth hormone (GH) system. In the 1887 experiment two issues were considered especially important. First, it was recognized that cells prepared from individual rat pituitary glands should be considered separately so that the data from the 5 glands could be analyzed in a statistically meaningful way. Second, results of the SL-3 flight involving the hollow fiber implant and HPLC GH-variant experiments suggested that the biological activity of the hormone had been negatively affected by flight. The results of the 1887 experiment documented the wisdom of addressing both issues in the protocol. Thus, the reduction in secretory capacity of flight cells during subsequent extended cell culture on Earth was documented statistically, and thereby established the validity of the SL-3 result. The results of both flight experiments thus support the contention that there is a secretory lesion in pituitary GH cells of flight animals. The primary objective of both missions was a clear definition of the effect of spaceflight on the GH cell system. There can no longer be any reasonable doubt that this system is affected in microgravity. One explanation for the reason(s) underlying the better known effects of spaceflight on organisms, viz. changes in bone, muscle and immune systems may very well rest with such changes in bGH. In spite of the fact that rats in the Cosmos 1887 flight were on Earth for two days after flight, the data show that the GH system had still not recovered from the effects of flight. Many questions remain. One of the more important concerns the GRF responsiveness of somatotrophs after flight. This will be tested in an upcoming experiment.

Grindeland, R.↗

Research Activities at NASA: “The Hera Astronaut Analog Mission, Astrobee, and the Advanced Composite Solar Sail System”

During this seminar, I will talk about three of the projects I’ve been involved with at NASA in the last few years: - The Human Exploration Research Analog (HERA) is a ground-based astronaut analog mission run at NASA’s JSC in Houston to study and evaluate impacts on the crew due to isolation, remoteness, and confined habitation. NASA scientists use the collected data to develop and verify countermeasures to reduce or mitigate psychological and physiological effects for future Deep Space missions. This simulation was a 45-day trip to Mars’s moon Phobos and back with the goal of performing geological operations with complete communications delays in effect. - Astrobee is a new class of free-flying robots that operates in the interior of the International Space Station (ISS). In addition to being a research platform for microgravity free-flying robotics, Astrobee improves the efficiency of ISS operations by providing flight and payload controllers with a mobile camera and a sensor platform. - NASA is developing new deployable structures and material technologies for solar sail propulsion systems destined for future low-cost deep space missions. NASA’s Advanced Composite Solar Sail System (ACS3) uses composite materials in its novel, lightweight booms that deploy from a Cubesat. Data obtained from ACS3 will guide the design of future larger-scale composite solar sail systems that could be used for several deep space exploration missions

International Space Station↗

Shear Thinning Near the Critical Point of Xenon

We measured shear thinning, a viscosity decrease ordinarily associated with complex liquids, near the critical point of xenon. The data span a wide range of reduced shear rate: 10(exp -3) < gamma-dot tau < 700, where gamma-dot tau is the shear rate scaled by the relaxation time tau of critical fluctuations. The measurements had a temperature resolution of 0.01 mK and were conducted in microgravity aboard the Space Shuttle Columbia to avoid the density stratification caused by Earth's gravity. The viscometer measured the drag on a delicate nickel screen as it oscillated in the xenon at amplitudes 3 mu,m < chi (sub 0) >430 mu, and frequencies 1 Hz < omega/2 pi < 5 Hz. To separate shear thinning from other nonlinearities, we computed the ratio of the viscous force on the screen at gamma-dot tau to the force at gamma-dot tau approximates 0: C(sub gamma) is identical with F(chi(sub 0), omega tau, gamma-dot tau )/F)(chi(sub 0, omega tau, 0). At low frequencies, (omega tau)(exp 2) < gamma-dot tau, C(sub gamma) depends only on gamma-dot tau, as predicted by dynamic critical scaling. At high frequencies, (omega tau)(exp 2) > gamma-dot tau, C(sub gamma) depends also on both x(sub 0) and omega. The data were compared with numerical calculations based on the Carreau-Yasuda relation for complex fluids: eta(gamma-dot)/eta(0)=[1+A(sub gamma)|gamma-dot tau|](exp - chi(sub eta)/3+chi(sub eta)), where chi(sub eta) =0.069 is the critical exponent for viscosity and mode-coupling theory predicts A(sub gamma) =0.121. For xenon we find A(sub gamma) =0.137 +/- 0.029, in agreement with the mode coupling value. Remarkably, the xenon data close to the critical temperature T(sub c) were independent of the cooling rate (both above and below T(sub c) and these data were symmetric about T(sub c) to within a temperature scale factor. The scale factors for the magnitude of the oscillator s response differed from those for the oscillator's phase; this suggests that the surface tension of the two-phase domains affected the drag on the screen below T(sub c).

Zimmerli, Gregory A.↗

Prediction of gas-liquid two-phase flow regime in microgravity

An attempt is made to predict gas-liquid two-phase flow regime in a pipe in a microgravity environment through scaling analysis based on dominant physical mechanisms. Simple inlet geometry is adopted in the analysis to see the effect of inlet configuration on flow regime transitions. Comparison of the prediction with the existing experimental data shows good agreement, though more work is required to better define some physical parameters. The analysis clarifies much of the physics involved in this problem and can be applied to other configurations.

Lee, Jinho↗

The Transient Dendritic Solidification Experiment (TDSE)

The study of steady-state dendritic growth has both validated many element of transport phenomena in dendritic growth, and yielded many new insights. Further development in simulation and modeling are needed, as is further understanding of the role of selection or scaling in dendritic growth. The TDSE contributes to the further study of dendritic phenomena by carefully measuring and modeling transient effects on dendritic growth. The major challenge encountered in measuring and analyzing the transient behavior of isothermal dendrites is defining precisely the initial conditions from which or to which the dendrite evolves. Our proposed pressure-mediated TDSE microgravity experiment, obviates this difficulty, because the transient occurs between two well-characterized steady-states, rather than between an ill-defined initial state and the final steady state. The major results expected are unique data on transient behavior that will extend the scientific bounds from the now well-understood thermal effects, and provide insight into interfacial dynamics where open questions remain.

Koss, M. B.↗