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At least 1,009 records · Page 56

Strengthening laboratory surveillance of viral pathogens: Experiences and lessons learned building next-generation sequencing capacity in Ghana

Objective—To demonstrate the feasibility of applying next-generation sequencing (NGS) in medium-resource reference laboratories in Africa to enhance global disease surveillance. Methods—A training program was developed to support implementation of NGS at Noguchi Memorial Institute for Medical Research (NMIMR), University of Ghana. The program was divided into two training stages, first at the Centers for Disease Control and Prevention (CDC) in Atlanta, GA, followed by on-site training at NMIMR for a larger cohort of scientists. Results—Self-assessment scores for topics covered during the NGS training program were higher post-training relative to pre-training. During the NGS Training II session at NMIMR, six enterovirus isolates from acute flaccid paralysis cases in Ghana were successfully sequenced by trainees, including two echovirus 6, two echovirus 11 and one echovirus 13. Another genome was an uncommon type (EV-B84), which has not been reported in Africa since its initial discovery from a Côte d’Ivoire specimen in 2003. Conclusions—The success at NMIMR provides an example of how to approach transferring of NGS methods to international laboratories. There is great opportunity for collaboration between institutes that have genomics expertise to ensure effectiveness and long-term success of global NGS capacity building programs.

60 APPLIED LIFE SCIENCES↗

Two deeply conserved non-coding sequences control PLETHORA1/2 expression and coordinate embryo and root development

Conserved non-coding sequences (CNSs) are integral elements of transcriptional regulation. Transcriptional tuning of PLETHORA (PLT) genes that encode master regulators of plant development is vital for embryogenesis and meristematic function. However, how the expression of PLT genes is modulated through CNSs remains unclear. Through motif-based mining of upstream sequences in 120 angiosperm genomes, we identified 21 conserved and lineage-specific CNSs, two of which are unusually long, similar, and colinear within eudicots. Using Arabidopsis thaliana, we demonstrate that these two deeply conserved elements, which we named BOX1 and BOX2, control PLT1 and PLT2 expression. CRISPR mutants within these elements specifically reduced PLT expression levels, and reporter lines revealed that deletion of either or both BOXes altered and/or abrogated the PLT2 expression pattern in the root tip, affecting the ability to rescue the plt1 plt2 double mutant. We further show that the influence of these elements on expression patterns is already exerted during embryogenesis and functional in the context of the early embryo. Finally, we reveal the existence of a BOX-mediated autoregulatory feedback loop that, in large part, explains CNS influence on expression patterns. We thus uncover a transcriptional mechanism by which genes encoding master regulators of embryo and root meristem development are regulated.

PLETHORA↗

One-Pot Self-Assembly of Sequence-Controlled Mesoporous Heterostructures via Structure-Directing Agents

Multimaterial heterostructures have led to characteristics surpassing the individual components. Nature controls the architecture and placement of multiple materials through biomineralization of nanoparticles (NPs); however, synthetic heterostructure formation remains limited and generally departs from the elegance of self-assembly. Here, in this study, a class of block polymer structure-directing agents (SDAs) are developed containing repeat units capable of persistent (covalent) NP interactions that enable the direct fabrication of nanoscale porous heterostructures, where a single material is localized at the pore surface as a continuous layer. This SDA binding motif (design rule 1) enables sequence-controlled heterostructures, where the composition profile and interfaces correspond to the synthetic addition order. This approach is generalized with 5 material sequences using an SDA with only persistent SDA-NP interactions (“P-NP 1 –NP 2 ”; NP i = TiO 2 , Nb 2 O 5 , ZrO 2 ). Expanding these polymer SDA design guidelines, it is shown that the combination of both persistent and dynamic (noncovalent) SDA-NP interactions (“PD-NP 1 –NP 2 ”) improves the production of uniform interconnected porosity (design rule 2). The resulting competitive binding between two segments of the SDA (P- vs D-) requires additional time for the first NP type (NP 1 ) to reach and covalently attach to the SDA (design rule 3). The combination of these three design rules enables the direct self-assembly of heterostructures that localize a single material at the pore surface while preserving continuous porosity.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Modeling Sequences of Earthquakes and Aseismic Slip (SEAS) in Elasto-Plastic Fault Zones With a Hybrid Finite Element Spectral Boundary Integral Scheme

We present a coupled finite element spectral boundary integral framework for modeling sequences of earthquakes and aseismic slip on a 2-D planar rate-and-state fault with off-fault visco-plastic response in the plane strain approximation. The model resolves both slow aseismic deformation and inertia effects during rapid slip. As an application, we perform two sets of simulations with different choices of cohesion to explore the co-evolution of fault slip, bulk plasticity and local stress fields. The first set implements a relatively large value of the cohesion parameter, which results in limiting inelastic strain accumulation to dynamic rupture phases. The second set implements a smaller cohesion, allowing for plastic strain to accumulate in both seismic and aseismic phases. For the first model, our results indicate that the extent and distribution of plastic strain depend on the angle of maximum compressive principal stress. At larger angles, inelastic strain accumulates on the extensional side of a dynamically propagating rupture. At smaller angles, the extent of plasticity is limited to the compressional side of the domain. At smaller cohesion values, off-fault plasticity may occur during aseismic slip, which alters the nucleation characteristics and earthquake sequence pattern. Furthermore, our results at lower cohesion values indicate that plastic strain accumulation may occur in both the extensional and compressional sides of the off-fault bulk even at higher angles of maximum compression. In conclusion, this produces damage patterns that deviate from the traditional off-fault fan-like distribution observed in dynamic rupture simulations and emphasizes the significance of long-term deformation in interpreting observations.

58 GEOSCIENCES↗

Computational evaluation of exome sequence data using human and model organism phenotypes improves diagnostic efficiency

Purpose: Medical diagnosis and molecular or biochemical confirmation typically rely on the knowledge of the clinician. Although this is very difficult in extremely rare diseases, we hypothesized that the recording of patient phenotypes in Human Phenotype Ontology (HPO) terms and computationally ranking putative disease-associated sequence variants improves diagnosis, particularly for patients with atypical clinical profiles. Methods: Using simulated exomes and the National Institutes of Health Undiagnosed Diseases Program (UDP) patient cohort and associated exome sequence, we tested our hypothesis using Exomiser. Exomiser ranks candidate variants based on patient phenotype similarity to (i) known disease–gene phenotypes, (ii) model organism phenotypes of candidate orthologs, and (iii) phenotypes of protein– protein association neighbors. Results: Benchmarking showed Exomiser ranked the causal variant as the top hit in 97% of known disease–gene associations and ranked the correct seeded variant in up to 87% when detectable disease–gene associations were unavailable. Using UDP data, Exomiser ranked the causative variant(s) within the top 10 variants for 11 previously diagnosed variants and achieved a diagnosis for 4 of 23 cases undiagnosed by clinical evaluation. Conclusion: Structured phenotyping of patients and computational analysis are effective adjuncts for diagnosing patients with genetic disorders.

semantic comparison↗

Unified rational protein engineering with sequence-based deep representation learning

Rational protein engineering requires a holistic understanding of protein function. Here, we apply deep learning to unlabeled amino-acid sequences to distill the fundamental features of a protein into a statistical representation that is semantically rich and structurally, evolutionarily and biophysically grounded. We show that the simplest models built on top of this unified representation (UniRep) are broadly applicable and generalize to unseen regions of sequence space. Our data-driven approach predicts the stability of natural and de novo designed proteins, and the quantitative function of molecularly diverse mutants, competitively with the state-of-the-art methods. UniRep further enables two orders of magnitude efficiency improvement in a protein engineering task. Lastly, UniRep is a versatile summary of fundamental protein features that can be applied across protein engineering informatics.

59 BASIC BIOLOGICAL SCIENCES↗

Influence of pH and sequence in peptide aggregation via molecular simulation

We employ a recently developed coarse-grained model for peptides and proteins where the effect of pH is automatically included. We explore the effect of pH in the aggregation process of the amyloidogenic peptide KTVIIE and two related sequences, using three different pH environments. Simulations using large systems (24 peptides chains per box) allow us to describe the formation of realistic peptide aggregates. We evaluate the thermodynamic and kinetic implications of changes in sequence and pH upon peptide aggregation, and we discuss how a minimalistic coarse-grained model can account for these details.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Sequence heterogeneity accelerates protein search for targets on DNA

The process of protein search for specific binding sites on DNA is fundamentally important since it marks the beginning of all major biological processes. We present a theoretical investigation that probes the role of DNA sequence symmetry, heterogeneity, and chemical composition in the protein search dynamics. Using a discrete-state stochastic approach with a first-passage events analysis, which takes into account the most relevant physical-chemical processes, a full analytical description of the search dynamics is obtained. It is found that, contrary to existing views, the protein search is generally faster on DNA with more heterogeneous sequences. In addition, the search dynamics might be affected by the chemical composition near the target site. The physical origins of these phenomena are discussed. Our results suggest that biological processes might be effectively regulated by modifying chemical composition, symmetry, and heterogeneity of a genome.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Emerging protein sequencing technologies: proteomics without mass spectrometry?

Liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been a leading method for proteomics for 30 years. Advantages provided by LC-MS/MS are offset by significant disadvantages, including cost. Recently, several non-mass spectrometric methods have emerged, but little information is available about their capacity to analyze the complex mixtures routine for mass spectrometry. Areas Covered: We review recent non-mass-spectrometric methods for sequencing proteins and peptides, including those using nanopores, sequencing by degradation, reverse translation, and short-epitope mapping, with comments on bioinformatics challenges, fundamental limitations, and areas where new technologies will be more or less competitive with LC-MS/MS. In addition to conventional literature searches, instrument vendor websites, patents, webinars, and preprints were also consulted to give a more up-to-date picture. Expert Opinion: Many new technologies are promising. However, demonstrations that they outperform mass spectrometry in terms of peptides and proteins identified have not yet been published, and astute observers note important disadvantages, especially relating to the dynamic range of single-molecule measurements of complex mixtures. Still, even if the performance of emerging methods proves inferior to LC-MS/MS, their low cost could create a different kind of revolution: a dramatic increase in the number of biology laboratories engaging in new forms of proteomics research.

59 BASIC BIOLOGICAL SCIENCES↗

ACCRETION RATES ON PRE-MAIN-SEQUENCE STARS IN THE YOUNG OPEN CLUSTER NGC 6530

It is well accepted that during the star formation process, material from a protoplanetary disk is accreted onto the central object during the first {approx}1-5 Myr. Different authors have published measurements of accretion rates for young low- and intermediate-mass stars in several nearby star-forming regions (SFRs). Due to its somewhat larger distance, the SFR M8 (the Lagoon Nebula) has not been studied to the same extent, despite its abundant population of young stellar objects. We have obtained optical band low-resolution spectra of a sample of pre-main-sequence stars in the open cluster NGC 6530 located in the aforementioned nebulae using the Gemini Multi Object Spectrograph at Gemini-South in multi-object mode. Spectra cover the H{sub {alpha}} emission line used to measure the accretion rate, following the method presented by Natta et al. The observed spectral characteristics are fully consistent with pre-main-sequence stars, showing lithium absorption lines, which are very common in young stellar objects, as well as prominent and broad H{sub {alpha}} emission lines, indicating a T Tauri evolutionary stage. This work presents the first determinations of mass accretion rates of young stellar objects in the open cluster NGC 6530, confirming that they are classical T Tauri stars going through the accretion phase. These observations contribute to a better understanding of the stellar content and evolutionary phase of the very active Lagoon Nebula SFR.

79 ASTRONOMY AND ASTROPHYSICS↗

PHOTOMETRIC DETERMINATION OF THE MASS ACCRETION RATES OF PRE-MAIN-SEQUENCE STARS. II. NGC 346 IN THE SMALL MAGELLANIC CLOUD

We have studied the properties of the stellar populations in the field of the NGC 346 cluster in the Small Magellanic Cloud, using a novel self-consistent method that allows us to reliably identify pre-main-sequence (PMS) objects actively undergoing mass accretion, regardless of their age. The method does not require spectroscopy and combines broadband V and I photometry with narrowband H{alpha} imaging to identify all stars with excess H{alpha} emission and derive the accretion luminosity L{sub acc} and mass accretion rate M-dot{sub acc} for all of them. The application of this method to existing Hubble Space Telescope (HST)/Advanced Camera for Surveys photometry of the NGC 346 field has allowed us to identify and study 680 bona fide PMS stars with masses from {approx}0.4 M{sub sun} to {approx}4 M{sub sun} and ages in the range from {approx}1 Myr to {approx}30 Myr. Previous investigations of this region, based on the same data, had identified young ({approx}3 Myr old) candidate PMS stars on the basis of their broadband colors. In this study, we show that there are at least two, almost equally numerous, young populations with distinct ages of, respectively, {approx}1 and {approx}20 Myr. We provide accurate physical parameters for all of them. We take advantage of the unprecedented size of our PMS sample and of its spread in mass and age to study the evolution of the mass accretion rate as a function of stellar parameters. We find that, regardless of stellar mass, the mass accretion rate decreases with roughly the square root of the age, or about three times slower than predicted by current models of viscous disk evolution, and that more massive stars systematically have a higher mass accretion rate in proportion to their mass. A multivariate linear regression fit reveals that log M-dot{sub acc}{approx_equal}-0.6 log t + log m + c, where t is the age of the star, m is its mass, and c is a quantity that is higher at lower metallicity. This result is consistent with measurements of the mass accretion rate in the 30 Dor region and in the Milky Way and suggests that the longer duration for mass accretion could be related to lower metallicity. The high-mass accretion rates that we find suggest that a considerable amount of mass is accreted during the PMS phase, of order {approx}0.2 M{sub sun} or possibly {approx}20% of the final mass for stars with mass m < 1 M{sub sun} if their disks are eroded by 20 Myr, i.e., before they reach the main sequence. Therefore, PMS evolutionary models that do not account for this effect will systematically underestimate the true age when compared with the observations.

79 ASTRONOMY AND ASTROPHYSICS↗

PSR J1903+0327: A UNIQUE MILLISECOND PULSAR WITH A MAIN-SEQUENCE COMPANION STAR

PSR J1903+0327 is a millisecond pulsar with a mass of 1.67 M{sub Sun} in a highly eccentric orbit (e = 0.44) around a main-sequence star. This unique system cannot be reconciled with current observations where millisecond pulsars are generally seen to orbit white dwarfs in almost exactly circular orbits. Current theoretical models of binary and stellar formation and evolution cannot explain the high eccentricity of this system either. In this work, we present three new epochs of optical spectroscopy for the companion to PSR J1903+0327, obtained to confirm the association of the main-sequence star with the pulsar. These three new epochs, together with the two previous ones, firmly establish the high eccentricity of the companion's orbit as predicted by pulsar timing. Using all five epochs of optical data, we have provided an independent estimate of the mass ratio, R = 1.56 {+-} 0.15, as well as the systemic radial velocity of the binary, {gamma} = 42.1 {+-} 2.5 km s{sup -1}. We constrain the spectral type of the pulsar companion to lie between F5 V and G0 V (a slightly earlier type than suggested previously) by measuring the equivalent widths of two of the three Ca-triplet lines (8498 A, 8542 A) and the Paschen line at 8598 A (P14); we also broadly constrain the metallicity of the companion. Additionally, we have placed a somewhat better limit on the rotational velocity of the pulsar companion of v{sub rot}sin i {<=} 66 km s{sup -1}, which is still not sufficient to allow a test of general relativity using this system.

79 ASTRONOMY AND ASTROPHYSICS↗

THE CLUSTERED NATURE OF STAR FORMATION. PRE-MAIN-SEQUENCE CLUSTERS IN THE STAR-FORMING REGION NGC 602/N90 IN THE SMALL MAGELLANIC CLOUD

Located at the tip of the wing of the Small Magellanic Cloud (SMC), the star-forming region NGC 602/N90 is characterized by the H II nebular ring N90 and the young cluster of pre-main-sequence (PMS) and early-type main-sequence stars NGC 602, located in the central area of the ring. We present a thorough cluster analysis of the stellar sample identified with Hubble Space Telescope/Advanced Camera for Surveys in the region. We show that apart from the central cluster low-mass PMS stars are congregated in 13 additional small, compact sub-clusters at the periphery of NGC 602, identified in terms of their higher stellar density with respect to the average background density derived from star counts. We find that the spatial distribution of the PMS stars is bimodal, with an unusually large fraction ({approx}60%) of the total population being clustered, while the remaining is diffusely distributed in the intercluster area, covering the whole central part of the region. From the corresponding color-magnitude diagrams we disentangle an age difference of {approx}2.5 Myr between NGC 602 and the compact sub-clusters, which appear younger, on the basis of comparison of the brighter PMS stars with evolutionary models, which we accurately calculated for the metal abundance of the SMC. The diffuse PMS population appears to host stars as old as those in NGC 602. Almost all detected PMS sub-clusters appear to be centrally concentrated. When the complete PMS stellar sample, including both clustered and diffused stars, is considered in our cluster analysis, it appears as a single centrally concentrated stellar agglomeration, covering the whole central area of the region. Considering also the hot massive stars of the system, we find evidence that this agglomeration is hierarchically structured. Based on our findings, we propose a scenario according to which the region NGC 602/N90 experiences an active clustered star formation for the last {approx}5 Myr. The central cluster NGC 602 was formed first and rapidly started dissolving into its immediate ambient environment, possibly ejecting also massive stars found away from its center. Star formation continued in sub-clusters of a larger stellar agglomeration, introducing an age spread of the order of 2.5 Myr among the PMS populations.

79 ASTRONOMY AND ASTROPHYSICS↗

EXTENDED MAGNETOSPHERES IN PRE-MAIN-SEQUENCE EVOLUTION: FROM T TAURI STARS TO THE BROWN DWARF LIMIT

Low-mass pre-main-sequence stars, i.e., T Tauri stars (TTSs), strongly radiate at high energies, from X-rays to the ultraviolet (UV). This excess radiation with respect to main-sequence cool stars (MSCSs) is associated with the accretion process, i.e., it is produced in the extended magnetospheres, in the accretion shocks on the stellar surface, and in the outflows. Although evidence of accretion shocks and outflow contribution to the high-energy excess have been recently addressed, there is not an updated revision of the magnetospheric contribution. This article addresses this issue. The UV observations of the TTSs in the well-known Taurus region have been analyzed together with the XMM-Newton observations compiled in the XEST survey. For the first time the high sensitivity of the Hubble Space Telescope UV instrumentation has allowed measurement of the UV line fluxes of TTSs to M8 type. UV- and X-ray-normalized fluxes have been determined to study the extent and properties of the TTS magnetospheres as a class. They have been compared with the atmospheres of the MSCSs. The main results from this analysis are (1) the normalized fluxes of all the tracers are correlated; this correlation is independent of the broad mass range and the hardness of the X-ray radiation field; (2) the TTS correlations are different than the MSCS correlations; (3) there is a very significant excess emission in O I in the TTSs compared with MSCSs that seems to be caused by recombination radiation from the disk atmosphere after photoionization by extreme UV radiation; the Fe II/Mg II recombination continuum has also been detected in several TTSs and most prominently in AA Tau; and (4) the normalized flux of the UV tracers anticorrelates with the strength of the X-ray flux, i.e., the stronger the X-ray surface flux is, the weaker the observed UV flux. This last behavior is counterintuitive within the framework of stellar dynamo theory and suggests that UV emission can be produced in the extended and dense stellar magnetosphere directly driven by local collisional processes. The brown dwarf 2MASS J12073346-3332539 has been found to follow the same flux-flux relations of the TTSs. Thus, TTS-normalized flux scaling laws seem to be extendable to the brown dwarf limit and can be used for identification/diagnosis purposes. We report the discovery of an inverse correlation between the C IV-normalized flux and the magnetospheric radius derived for stars with known magnetic fields. The normalized C IV flux is found to be {proportional_to}exp (- {alpha}r{sub mag}), with {alpha} = 0.5-0.7.

79 ASTRONOMY AND ASTROPHYSICS↗

CAN WE PREDICT THE GLOBAL MAGNETIC TOPOLOGY OF A PRE-MAIN-SEQUENCE STAR FROM ITS POSITION IN THE HERTZSPRUNG-RUSSELL DIAGRAM?

Zeeman-Doppler imaging studies have shown that the magnetic fields of T Tauri stars can be significantly more complex than a simple dipole and can vary markedly between sources. We collect and summarize the magnetic field topology information obtained to date and present Hertzsprung-Russell (H-R) diagrams for the stars in the sample. Intriguingly, the large-scale field topology of a given pre-main-sequence (PMS) star is strongly dependent upon the stellar internal structure, with the strength of the dipole component of its multipolar magnetic field decaying rapidly with the development of a radiative core. Using the observational data as a basis, we argue that the general characteristics of the global magnetic field of a PMS star can be determined from its position in the H-R diagram. Moving from hotter and more luminous to cooler and less luminous stars across the PMS of the H-R diagram, we present evidence for four distinct magnetic topology regimes. Stars with large radiative cores, empirically estimated to be those with a core mass in excess of {approx}40% of the stellar mass, host highly complex and dominantly non-axisymmetric magnetic fields, while those with smaller radiative cores host axisymmetric fields with field modes of higher order than the dipole dominant (typically, but not always, the octupole). Fully convective stars above {approx}> 0.5 M{sub Sun} appear to host dominantly axisymmetric fields with strong (kilo-Gauss) dipole components. Based on similarities between the magnetic properties of PMS stars and main-sequence M-dwarfs with similar internal structures, we speculate that a bistable dynamo process operates for lower mass stars ({approx}< 0.5 M{sub Sun} at an age of a few Myr) and that they will be found to host a variety of magnetic field topologies. If the magnetic topology trends across the H-R diagram are confirmed, they may provide a new method of constraining PMS stellar evolution models.

79 ASTRONOMY AND ASTROPHYSICS↗

STELLAR DIAMETERS AND TEMPERATURES. II. MAIN-SEQUENCE K- AND M-STARS

We present interferometric angular diameter measurements of 21 low-mass, K- and M-dwarfs made with the CHARA Array. This sample is enhanced by adding a collection of radius measurements published in the literature to form a total data set of 33 K-M-dwarfs with diameters measured to better than 5%. We use these data in combination with the Hipparcos parallax and new measurements of the star's bolometric flux to compute absolute luminosities, linear radii, and effective temperatures for the stars. We develop empirical relations for {approx}K0 to M4 main-sequence stars that link the stellar temperature, radius, and luminosity to the observed (B - V), (V - R), (V - I), (V - J), (V - H), and (V - K) broadband color index and stellar metallicity [Fe/H]. These relations are valid for metallicities ranging from [Fe/H] = -0.5 to +0.1 dex and are accurate to {approx}2%, {approx}5%, and {approx}4% for temperature, radius, and luminosity, respectively. Our results show that it is necessary to use metallicity-dependent transformations in order to properly convert colors into stellar temperatures, radii, and luminosities. Alternatively, we find no sensitivity to metallicity on relations we construct to the global properties of a star omitting color information, e.g., temperature-radius and temperature-luminosity. Thus, we are able to empirically quantify to what order the star's observed color index is impacted by the stellar iron abundance. In addition to the empirical relations, we also provide a representative look-up table via stellar spectral classifications using this collection of data. Robust examinations of single star temperatures and radii compared to evolutionary model predictions on the luminosity-temperature and luminosity-radius planes reveal that models overestimate the temperatures of stars with surface temperatures <5000 K by {approx}3%, and underestimate the radii of stars with radii <0.7 R{sub Sun} by {approx}5%. These conclusions additionally suggest that the models over account for the effects that the stellar metallicity may have on the astrophysical properties of an object. By comparing the interferometrically measured radii for the single star population to those of eclipsing binaries, we find that for a given mass, single and binary star radii are indistinguishable. However, we also find that for a given radius, the literature temperatures for binary stars are systematically lower compared to our interferometrically derived temperatures of single stars by {approx}200 to 300 K. The nature of this offset is dependent on the validation of binary star temperatures, where bringing all measurements to a uniform and correctly calibrated temperature scale is needed to identify any influence stellar activity may have on the physical properties of a star. Lastly, we present an empirically determined H-R diagram using fundamental properties presented here in combination with those in Boyajian et al. for a total of 74 nearby, main-sequence, A- to M-type stars, and define regions of habitability for the potential existence of sub-stellar mass companions in each system.

79 ASTRONOMY AND ASTROPHYSICS↗

MASS LOSS IN PRE-MAIN-SEQUENCE STARS VIA CORONAL MASS EJECTIONS AND IMPLICATIONS FOR ANGULAR MOMENTUM LOSS

We develop an empirical model to estimate mass-loss rates via coronal mass ejections (CMEs) for solar-type pre-main-sequence (PMS) stars. Our method estimates the CME mass-loss rate from the observed energies of PMS X-ray flares, using our empirically determined relationship between solar X-ray flare energy and CME mass: log (M {sub CME}[g]) = 0.63 Multiplication-Sign log (E {sub flare}[erg]) - 2.57. Using masses determined for the largest flaring magnetic structures observed on PMS stars, we suggest that this solar-calibrated relationship may hold over 10 orders of magnitude in flare energy and 7 orders of magnitude in CME mass. The total CME mass-loss rate we calculate for typical solar-type PMS stars is in the range 10{sup -12}-10{sup -9} M {sub Sun} yr{sup -1}. We then use these CME mass-loss rate estimates to infer the attendant angular momentum loss leading up to the main sequence. Assuming that the CME outflow rate for a typical {approx}1 M {sub Sun} T Tauri star is <10{sup -10} M {sub Sun} yr{sup -1}, the resulting spin-down torque is too small during the first {approx}1 Myr to counteract the stellar spin-up due to contraction and accretion. However, if the CME mass-loss rate is {approx}> 10{sup -10} M {sub Sun} yr{sup -1}, as permitted by our calculations, then the CME spin-down torque may influence the stellar spin evolution after an age of a few Myr.

79 ASTRONOMY AND ASTROPHYSICS↗

PHIBSS: MOLECULAR GAS CONTENT AND SCALING RELATIONS IN z {approx} 1-3 MASSIVE, MAIN-SEQUENCE STAR-FORMING GALAXIES

We present PHIBSS, the IRAM Plateau de Bure high-z blue sequence CO 3-2 survey of the molecular gas properties in massive, main-sequence star-forming galaxies (SFGs) near the cosmic star formation peak. PHIBSS provides 52 CO detections in two redshift slices at z {approx} 1.2 and 2.2, with log(M{sub *}(M{sub Sun })) {>=} 10.4 and log(SFR(M{sub Sun }/yr)) {>=} 1.5. Including a correction for the incomplete coverage of the M{sub *} -SFR plane, and adopting a ''Galactic'' value for the CO-H{sub 2} conversion factor, we infer average gas fractions of {approx}0.33 at z {approx} 1.2 and {approx}0.47 at z {approx} 2.2. Gas fractions drop with stellar mass, in agreement with cosmological simulations including strong star formation feedback. Most of the z {approx} 1-3 SFGs are rotationally supported turbulent disks. The sizes of CO and UV/optical emission are comparable. The molecular-gas-star-formation relation for the z = 1-3 SFGs is near-linear, with a {approx}0.7 Gyr gas depletion timescale; changes in depletion time are only a secondary effect. Since this timescale is much less than the Hubble time in all SFGs between z {approx} 0 and 2, fresh gas must be supplied with a fairly high duty cycle over several billion years. At given z and M{sub *}, gas fractions correlate strongly with the specific star formation rate (sSFR). The variation of sSFR between z {approx} 0 and 3 is mainly controlled by the fraction of baryonic mass that resides in cold gas.

79 ASTRONOMY AND ASTROPHYSICS↗